CN111388522B - Plant essence for preventing, treating and repairing scar hyperplasia and preparation method thereof - Google Patents

Plant essence for preventing, treating and repairing scar hyperplasia and preparation method thereof Download PDF

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CN111388522B
CN111388522B CN202010235022.1A CN202010235022A CN111388522B CN 111388522 B CN111388522 B CN 111388522B CN 202010235022 A CN202010235022 A CN 202010235022A CN 111388522 B CN111388522 B CN 111388522B
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CN111388522A (en
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孟令超
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Chenfeng Natural Herbal Beijing Technology Co ltd
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Abstract

The invention discloses a plant essence for preventing and repairing scar hyperplasia and a preparation method thereof, wherein the plant essence comprises the following raw materials in percentage by weight: 0.5-10% of pseudo-ginseng extract powder, 0.5-10% of licorice extract powder, 0.5-10% of sophora flower bud extract powder and the balance of solvent, wherein the total weight percentage of the raw materials is 100%. The invention can effectively activate immune cell population of a wound part, inhibit macrophage polarization, inhibit expression of inflammatory factors and trend factors, inhibit proliferation and differentiation of fibroblasts, reduce ECM (extracellular matrix) deposition, promote regeneration of capillary vessels around pathological changes, improve microcirculation, prevent and repair scar hyperplasia from inside to outside, and has very high safety.

Description

Plant essence for preventing, treating and repairing scar hyperplasia and preparation method thereof
Technical Field
The invention relates to the field of biological medicines, in particular to a plant essence for preventing and repairing scar hyperplasia and a preparation method thereof.
Background
Scars are one of the most common complications after wound healing and are always a difficult point and a hot point for research in the field of surgical repair. Not only does excessive scar proliferation affect the cosmetic appearance of the patient, but the resulting spasms can lead to varying degrees of dysfunction, placing a heavy economic and psychological burden on the later treatment of such patients.
Scar formation is actually only a stage in skin trauma, and therefore understanding the process of skin healing is a critical loop for scar repair. From a pathological point of view, healing of skin wounds generally falls into four stages: hemostasis, inflammatory responses, cell proliferation/matrix precipitation and skin tissue remodeling, these phases being mediated by a cascade of immune cells and signaling molecules. In the process of skin wound healing, fibroblasts, keratinocytes and epithelial cells are accumulated at the site of injury, and scar formation begins in the third phase of wound healing. In this stage, fibroblasts differentiate into myofibroblasts, which secrete and synthesize new ECM (extracellular matrix). The new ECM is remodeled to form scar tissue. Meanwhile, cytokines secreted by leukocytes play a direct role not only in the initial aggregation of fibroblasts, but also play a key role in mediating the development of scar formation in general inflammatory responses. At present, the commonly used treatment techniques in the aspect of scar treatment include surgical operations such as skin grafting, laser treatment, skin dilator treatment, excision and suture, grinding, treatment by using simple hormone drugs such as dexamethasone, freezing treatment and the like. The technologies relieve the symptoms of patients to different degrees, but the non-drug treatment in the methods has the defects of higher cost, more pain of patients, long operation treatment time and the like, and the diseases are easy to relapse after recovery. The treatment with the steroid hormone medicines such as dexamethasone may cause harm to the body and even cause sterility, osteoporosis and the like.
The existing products for preventing and repairing various scars have certain limitations. The invention provides a plant essence for preventing and repairing scar hyperplasia and a preparation method thereof. The product combines the traditional Chinese herbal medicine and western pharmacology, selects the traditional Chinese medicines of pseudo-ginseng, liquorice and sophora flower which have the functions of removing blood stasis, promoting tissue regeneration, resisting inflammation, sterilizing, activating immunity, whitening and lightening spots, and enriches dammarane type tetracyclic pentaplenin (ginsenoside Rb) by an extraction and purification technology1、Rg1Rd, notoginsenoside R1Etc.), glycyrrhizin and flavone, rutin and plant polysaccharide, etc., can effectively activate immune cell population of wound parts, inhibit macrophage polarization, inhibit expression of inflammatory factors and trend factors, inhibit proliferation and differentiation of fibroblasts, reduce ECM (extracellular matrix) deposition, promote regeneration of capillary vessels around pathological changes, improve microcirculation, prevent and repair scar hyperplasia from inside to outside, and has high safety.
Disclosure of Invention
The invention aims to provide a plant essence for preventing and repairing scar hyperplasia and a preparation method thereof, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the plant essence for preventing and repairing scar hyperplasia comprises the following raw materials in percentage by weight: 0.5-10% of pseudo-ginseng extract powder, 0.5-10% of licorice extract powder, 0.5-10% of sophora flower bud extract powder and the balance of solvent, wherein the sum of the weight percentages of the raw materials and the solvent is 100%.
In some embodiments, the botanical essence comprises the following raw materials in weight percent: 2-10% of pseudo-ginseng extract powder, 2-10% of licorice extract powder, 2-10% of sophora flower bud extract powder and the balance of solvent, wherein the sum of the weight percentages of the raw materials and the solvent is 100%.
In some embodiments, the solvent is a mixture of 60% to 90% by weight of glycerol and deionized water.
In some embodiments, the solvent is a mixture of 60% to 90% by weight of 1, 3 propanediol and deionized water.
In some embodiments, the solvent is a mixture of 60 to 90 weight percent 1, 3 butanediol and deionized water.
In some embodiments, the panax notoginseng extract powder is prepared by the following method:
1) grinding Notoginseng radix into powder with a pulverizer, and sieving with a 20 mesh sieve to obtain raw material powder for use;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain the pseudo-ginseng extract powder.
In some embodiments, the ginseng extract powder is prepared by:
1) grinding ginseng roots into powder by using a grinder, and sieving the powder by using a 20-mesh sieve to obtain raw material powder for later use;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain the ginseng extract powder.
In some embodiments, the licorice extract powder is prepared by the following method:
1) grinding Glycyrrhrizae radix into powder with a grinder, and sieving with 20 mesh sieve to obtain raw material powder;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain licorice extract powder.
In some embodiments, the sophora flower bud extract powder is prepared by the following method:
1) pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain sophora flower bud extract powder.
The invention also provides a preparation method of the plant essence, which is characterized by comprising the following steps:
1) grinding Notoginseng radix into powder with a pulverizer, and sieving with a 20 mesh sieve to obtain raw material powder for use; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing the two filtrates, concentrating until the solid content is 10-20%, and performing spray drying to obtain radix Notoginseng extract powder for later use;
2) grinding ginseng roots into powder by using a grinder, and sieving the powder by using a 20-mesh sieve to obtain raw material powder for later use; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain ginseng extract powder for later use;
3) grinding Glycyrrhrizae radix into powder with a grinder, and sieving with 20 mesh sieve to obtain raw material powder; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain licorice extract powder for later use;
4) pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; and combining and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain sophora flower bud extract powder for later use.
In some embodiments, the plant essence of the present invention is prepared by the following method: mixing 0.5-10% of pseudo-ginseng extract powder, 0.5-10% of licorice extract powder and 0.5-10% of sophora flower bud extract powder. And then adding the rest solvent into the mixed extract powder, heating to 45-80 ℃, refluxing, stirring until the mixture is completely dissolved, adding 5-20% of activated carbon for decolorization, filtering, cooling the filtrate to room temperature, standing for 1-3 days, and filtering again to obtain a finished product.
Compared with the prior art, the invention has the characteristics and advantages that:
the invention is a plant formula product, and the combined formula is not reported in other related scientific researches, and has uniqueness and originality. The product combines the traditional Chinese herbal medicine and western pharmacology, selects the traditional Chinese medicines of notoginseng, ginseng, liquorice and sophora flower bud with the functions of removing blood stasis, promoting tissue regeneration, resisting inflammation, sterilizing, activating immunity, whitening and lightening spots, and enriches the effective active ingredient dammarane type tetracyclic pentapenoid saponins (ginsenoside Rb) by an extraction and purification technology1、Rg1Rd, notoginsenoside R1Etc.), glycyrrhizin and flavone, rutin and plant polysaccharide, etc., can effectively activate immune cell population of wound parts, inhibit macrophage polarization, inhibit expression of inflammatory factors and trend factors, inhibit proliferation and differentiation of fibroblasts, reduce ECM (extracellular matrix) deposition, simultaneously promote regeneration of capillary vessels around pathological changes, improve microcirculation, prevent and cure and repair scar hyperplasia from inside to outside, and have very high safety.
Drawings
FIG. 1: the action mechanism diagram of the plant essence for preventing, treating and repairing scar hyperplasia is provided.
FIG. 2: a bar graph reflecting the inhibitory effect of example 2 on TNF- α, IL-6 and IL-8, inflammatory factors produced by LPS-induced RAW264.7 cells.
FIG. 3: histogram reflecting the inhibitory effect of example 2 on scar area generation in rabbit ear model.
FIG. 4: histogram reflecting the inhibitory effect of example 2 on collagen I concentration in scar tissue in rabbit ear model.
FIG. 5: histogram reflecting the inhibitory effect of example 2 on collagen III concentration in scar tissue in rabbit ear model.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Example 1
The plant essence for preventing and repairing scar hyperplasia comprises the following raw materials in percentage by weight: 2% of pseudo-ginseng extract powder, 2% of licorice extract powder, 2% of sophora flower bud extract powder, 15% of glycerol and 77% of deionized water, wherein the total weight percentage of the raw materials is 100%.
A preparation method of plant essence for preventing and repairing scar hyperplasia comprises the following steps:
(1) grinding Notoginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Notoginseng radix extract powder.
(2) Pulverizing Ginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Ginseng radix extract powder.
(3) Pulverizing Glycyrrhrizae radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Glycyrrhrizae radix extract powder.
(4) Pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until the solid content is 15%, and spray drying to obtain flos Sophorae Immaturus extract powder for use.
(5) Mixing 2% of the panax notoginseng extract powder, 2% of the ginseng extract powder, 2% of the liquorice extract powder and 2% of the sophora flower bud extract powder according to the total mass percentage of the raw materials.
(6) And then adding 15% of glycerol and 77% of deionized water into the mixed extract powder, heating to 60 ℃, refluxing, stirring until the mixture is completely dissolved, adding 10% of activated carbon for decolorization, filtering, cooling the filtrate to room temperature, standing for 2 days, and filtering again to obtain a finished product.
Example 2
The plant essence for preventing and repairing scar hyperplasia comprises the following raw materials in percentage by weight: 5% of pseudo-ginseng extract powder, 5% of licorice extract powder, 5% of sophora flower bud extract powder, 10% of 1, 2-propylene glycol and 70% of deionized water, wherein the sum of the weight percentages of the raw materials is 100%.
A preparation method of plant essence for preventing and repairing scar hyperplasia comprises the following steps:
(1) grinding Notoginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Notoginseng radix extract powder.
(2) Pulverizing Ginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Ginseng radix extract powder.
(3) Pulverizing Glycyrrhrizae radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Glycyrrhrizae radix extract powder.
(4) Pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until the solid content is 15%, and spray drying to obtain flos Sophorae Immaturus extract powder for use.
(5) Mixing 5% of the panax notoginseng extract powder, 5% of the ginseng extract powder, 5% of the liquorice extract powder and 5% of the sophora flower bud extract powder according to the total mass percentage of the raw materials.
(6) Then adding 10% of 1, 2-propylene glycol and 70% of deionized water into the mixed extract powder, heating to 50 ℃ for refluxing, stirring until the mixture is completely dissolved, adding 15% of activated carbon for decoloring, filtering, cooling the filtrate to room temperature, standing for 3 days, and filtering again to obtain the finished product.
Example 3
The plant essence for preventing and repairing scar hyperplasia comprises the following raw materials in percentage by weight: 10% of pseudo-ginseng extract powder, 10% of liquorice extract powder, 10% of sophora flower bud extract powder, 18% of 1, 3-butanediol and 42% of deionized water, wherein the sum of the weight percentages of the raw materials is 100%.
A preparation method of plant essence for preventing and repairing scar hyperplasia comprises the following steps:
(1) grinding Notoginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder for use. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Notoginseng radix extract powder.
(2) Pulverizing Ginseng radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Ginseng radix extract powder.
(3) Pulverizing Glycyrrhrizae radix into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until solid content is 15%, and spray drying to obtain Glycyrrhrizae radix extract powder.
(4) Pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with 20 mesh sieve to obtain raw material powder. Then, the raw material powder was added to 6 times of deionized water, stirred and extracted at 90 ℃ for 4 hours, and filtered to obtain a primary filtrate. And adding deionized water with the weight being 4 times that of the primary extraction slag, stirring and extracting for 2 hours at the temperature of 90 ℃, and filtering to obtain secondary filtrate. Mixing the two filtrates, concentrating until the solid content is 15%, and spray drying to obtain flos Sophorae Immaturus extract powder for use.
(5) Mixing 10% of the panax notoginseng extract powder, 10% of the ginseng extract powder, 10% of the liquorice extract powder and 10% of the sophora flower bud extract powder according to the total mass percentage of the raw materials.
(6) Then adding 15% of 1, 3-butanediol and 45% of deionized water into the mixed extract powder, heating to 60 ℃, refluxing, stirring until the mixture is completely dissolved, adding 20% of activated carbon for decolorization, filtering, cooling the filtrate to room temperature, standing for 3 days, and filtering again to obtain the finished product.
The product of the invention is rich in dammarane type tetracyclic pentadecanoid saponins (ginsenoside Rb)1、Rg1Rd, notoginsenoside R1Etc.), glycyrrhizin and flavone, rutin and plant polysaccharide, etc., can effectively activate immune cell population of wound parts, inhibit macrophage polarization, inhibit expression of inflammatory factors and trend factors, inhibit proliferation and differentiation of fibroblasts, reduce ECM (extracellular matrix) deposition, promote regeneration of capillary vessels around pathological changes, improve microcirculation, prevent and cure scar hyperplasia from inside to outside, etc. The possible mechanism of action is shown in figure 1. In order to verify the effect of the plant essence of the invention in preventing and repairing scar hyperplasia, the following tests are carried out:
anti-inflammatory Activity assay
A large number of scientific research reports prove that the generation of acute and chronic inflammation, wound healing and scar formation have direct and indirect connection, inflammatory factors can mediate the proliferation and differentiation of fibroblasts, and promote the secretion and deposition of ECM, so that the inflammatory factors are key targets for causing scar hyperplasia. Therefore, the inhibition and the reduction of inflammatory reaction are important links for preventing and repairing scar hyperplasia. Among them, TNF-alpha, IL-6 and IL-8 are key inflammatory expression factors.
This assay used LPS (Lipopolysaccharide, using a concentration of 1. mu.g/ml) to induce an inflammatory response in RAW264.7 (mouse mononuclear macrophages), treated with the product of example 2 of the present invention (using a concentration of 500ug/ml), and finally evaluated for the expression of the inflammatory factors TNF-alpha, IL-6, IL-8 using ELISA (Enzyme linked immunosorbent assay). The test results are shown in FIG. 2.
From the test results, when RAW264.7 is induced by LPS, the expressions of TNF-alpha, IL-6 and IL-8 are remarkably increased compared with a blank Control group (Control), which indicates that inflammation is induced by LPS, and when the product is treated by the product of the invention in the concentration of 500 mug/ml, the expressions of TNF-alpha, IL-6 and IL-8 are greatly reduced, which indicates that the product of the invention has remarkable inhibition effect on the expression of inflammatory factors induced by LPS.
Second, animal body test for inhibiting scar hyperplasia (rabbit ear model)
1. Influence on scar area
The test uses 10 healthy New Zealand rabbits (2.0-2.2 kg body weight) which are divided equally into two groups: a circular puncture wound (D ═ 1CM) was made in the ear belly of both rabbits in the blank group (5) and treatment group (5) to create a scar model. Starting on day 14 of the wound, the treatment group treated the wound daily with a 2% by weight sterile aqueous solution containing example 2 for 10 days, and the blank group was treated with an equal amount of 1% physiological saline for 10 days. Scar area differences were measured at day 30. The results are shown in FIG. 3 as the mean standard deviation.
From the test results, the average scar area of the treatment group treated by the example 2 is reduced by 38% compared with that of the blank group, which shows that the product of the invention has the function of inhibiting the generation of scars and can obviously reduce the hyperplasia of scars.
2. Influence on collagen synthesis
In the wound healing process, the production of collagen can be said to be a twolip sword, which is essential for the healing process on the one hand; on the other hand, overproduction and excessive deposition of collagen can lead to excessive scar proliferation. While the production of collagen and ECM is mainly mediated by the hyperproliferation and secretion of myofibroblasts. Numerous reports on scar-related scientific research have also demonstrated that: by inhibiting proliferation and differentiation of fibroblasts, excessive production of collagen and ECM can be inhibited, thereby effectively inhibiting proliferation of scar tissue. In the test, scar tissues in a healthy group, a control group and a treatment group treated in the embodiment 2 of the rabbit ear model are sampled, and the content of collagen I and collagen III is measured to evaluate the influence of the product on the collagen, so that the inhibition effect of the product on scar hyperplasia is further verified.
In the two groups of rabbit ear models, hypertrophic scar tissue of one rabbit ear in each group is sampled respectively at 7 th day and 30 th day after treatment, and any healthy part tissue is sampled simultaneously, namely, the rabbit ears are divided into a healthy group, a blank group and a treatment group at each time point. After the sample is processed, the processed sample is subjected to content measurement of collagen I and collagen III by ELISA (Enzyme linked immunosorbent assay), and the test results are shown in fig. 4 and fig. 5.
From the test results, the contents of collagen I and collagen III in the scar tissue of the treatment group treated by the product of the invention are greatly reduced compared with the blank group, and the concentration of the scar tissue is closer to that of the healthy group. This shows that the test product can effectively inhibit the generation of collagen, thereby inhibiting the excessive proliferation of scars.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. The plant essence for preventing and repairing scar hyperplasia is characterized by comprising the following raw materials in percentage by weight: 2-10% of pseudo-ginseng extract powder, 2-10% of licorice extract powder, 2-10% of sophora flower bud extract powder and the balance of solvent, wherein the sum of the weight percentages of the raw materials and the solvent is 100%.
2. The plant essence according to claim 1, wherein the plant essence comprises the following raw materials in percentage by weight: 5% of pseudo-ginseng extract powder, 5% of licorice extract powder, 5% of sophora flower bud extract powder and the balance of solvent, wherein the sum of the weight percentages of the raw materials and the solvent is 100%.
3. The plant essence according to claim 1, wherein the solvent is a mixture of 60-90 wt% of glycerin and deionized water.
4. The plant essence according to claim 1, wherein the solvent is a mixture of 60-90 wt% of 1, 3-propanediol and deionized water.
5. The plant essence according to claim 1, wherein the solvent is a mixture of 60 to 90 weight percent of 1, 3-butanediol and deionized water.
6. The plant essence according to claim 1, wherein the notoginseng extract powder is prepared by the following method:
1) grinding Notoginseng radix into powder with a pulverizer, and sieving with a 20 mesh sieve to obtain raw material powder for use;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain the pseudo-ginseng extract powder.
7. The plant essence of claim 1, wherein the ginseng extract powder is prepared by the following method:
1) grinding ginseng roots into powder by using a grinder, and sieving the powder by using a 20-mesh sieve to obtain raw material powder for later use;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain the ginseng extract powder.
8. The plant essence of claim 1, wherein the licorice extract powder is prepared by the following method:
1) grinding Glycyrrhrizae radix into powder with a grinder, and sieving with 20 mesh sieve to obtain raw material powder;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain licorice extract powder.
9. The plant essence of claim 1, wherein the sophora flower bud extract powder is prepared by the following method:
1) pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder;
2) adding raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate and primary extraction slag;
3) adding deionized water with the weight being 3-6 times that of the primary extracted material slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate and secondary extracted material slag;
4) and (3) combining the primary filtrate and the secondary filtrate, concentrating until the solid content is 10-20%, and performing spray drying to obtain sophora flower bud extract powder.
10. The preparation method of the plant essence for preventing and repairing scar hyperplasia according to any one of claims 1-4, which comprises the following steps:
1) grinding Notoginseng radix into powder with a pulverizer, and sieving with a 20 mesh sieve to obtain raw material powder for use; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing the two filtrates, concentrating until the solid content is 10-20%, and performing spray drying to obtain radix Notoginseng extract powder for later use;
2) grinding ginseng roots into powder by using a grinder, and sieving the powder by using a 20-mesh sieve to obtain raw material powder for later use; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain ginseng extract powder for later use;
3) grinding Glycyrrhrizae radix into powder with a grinder, and sieving with 20 mesh sieve to obtain raw material powder; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; mixing and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain licorice extract powder for later use;
4) pulverizing flos Sophorae Immaturus into powder with a pulverizer, and sieving with a 20-mesh sieve to obtain raw material powder; then adding the raw material powder into deionized water with the weight 5-8 times of that of the raw material powder, stirring and extracting for 4-6 hours at the temperature of 60-90 ℃, and filtering to obtain primary filtrate; adding deionized water with the weight being 3-6 times that of the primary extraction slag, stirring and extracting for 2-4 hours at the temperature of 60-90 ℃, and filtering to obtain secondary filtrate; and combining and concentrating the two filtrates until the solid content is 10-20%, and performing spray drying to obtain sophora flower bud extract powder for later use.
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