CN111378005A

CN111378005A - Adjustable protein purifying device

Info

Publication number: CN111378005A
Application number: CN202010260782.8A
Authority: CN
Inventors: 雍金贵; 李森; 张伦
Original assignee: Anhui Global Gene Technology Co ltd
Current assignee: Anhui Global Gene Technology Co ltd
Priority date: 2020-04-03
Filing date: 2020-04-03
Publication date: 2020-07-07
Anticipated expiration: 2040-04-03
Also published as: CN111378005B

Abstract

The invention discloses an adjustable protein purification device, which is characterized in that a pressure plate is arranged at one end of a filtering membrane, the pressure plate is arranged at an acute included angle with the vertical direction under a non-working state, when the protein filtration and separation process needs to be adjusted, a driving cylinder drives a movable rod to move upwards, the movable rod pushes the pressure plate to move upwards through a first base, the pressure plate further moves upwards to be attached to a guide plate, the filtering membrane is pressed by the pressure plate, a conduit is separated from a storage box through the attachment of the pressure plate, so that the protein is not filtered any more, the protein can be fully centrifugally separated in the centrifugal box as required, the centrifugal separation degree of the protein in the centrifugal box can be adjusted through the driving of the driving cylinder, and the protein purification degree entering the storage box through the conduit can be adjusted as required, thereby greatly improving the efficiency of protein purification.

Description

Adjustable protein purifying device

Technical Field

The invention relates to the field of protein purification devices, in particular to an adjustable protein purification device, and belongs to the technical field of application of adjustable protein purification devices.

Background

Protein purification uses the inherent similarity and difference between different proteins, uses the similarity between various proteins to remove contamination of non-protein substances, and uses the difference between proteins to purify a target protein from other proteins. The size, shape, charge, hydrophobicity, solubility and biological activity of each protein can vary, and the protein can be extracted from a mixture such as E.coli lysate to obtain recombinant protein. The purpose of separation by centrifugal rotation during protein purification is one of the common modes, and when the centrifugal rotation is carried out in the operation of the conventional protein purification, the filtering process in the centrifugal process can not be adjusted, so that when the filtration with different purities is required, the conventional protein purification can not be achieved by centrifugal rotation, and the use requirement can not be met.

Disclosure of Invention

The invention aims to provide an adjustable protein purification device, which aims to solve the technical problems that when the conventional protein purification operation is performed in centrifugal rotation, the filtration process in the centrifugal process cannot be adjusted, so that when filtration with different purities is required, the conventional protein purification cannot be achieved through centrifugal rotation, and the use requirement cannot be met.

The purpose of the invention can be realized by the following technical scheme:

a tunable protein purification device, comprising: the centrifugal box comprises a box body, a storage box, a centrifugal box, a supporting rod, a driving motor, a supporting plate, a driving shaft, a bearing plate, a rotating plate, a guide plate, a filtering membrane, a pressing plate, a movable rod and a driving cylinder, wherein the storage box is fixedly arranged at the upper end of the box body, the storage box is of a barrel-shaped structure, the rotating plate is horizontally arranged in the storage box, the centrifugal box is fixedly arranged at the top end of the rotating plate through a bolt, the bottom end of the rotating plate is fixedly connected with the driving shaft through a bolt, the bottom end of the driving shaft penetrates through the storage box and extends downwards, the bottom end of the storage box is horizontally provided with a supporting plate, the bottom end of the driving shaft is rotatably connected with a guide sleeve through a bearing, the guide sleeve is fixedly connected with a bearing plate through a bolt, the driving shaft is rotatably connected with the supporting plate through a guide sleeve, the bearing plate is fixedly connected with the supporting plate through a bolt, the bottom end of the supporting plate is fixedly provided with a driving motor through a bolt, and the;

the bottom end of the centrifugal box is provided with a plurality of guide pipes at equal intervals along the circumferential direction, the guide pipes are all communicated with the centrifugal box, the guide pipes are of an L-shaped pipe-shaped structure, one end of each guide pipe is integrally connected with a fixed plate, one end of the fixed plate is fixedly provided with a guide plate, the lower end of the guide plate is provided with a filtering hole, the size of the filtering hole is matched with the aperture of each guide pipe, the guide plate is fixedly connected with the guide pipes through the filtering holes, one end of the guide plate is fixedly provided with a pin shaft through a mounting hole, one end of the pin shaft is abutted against the guide pipes, one end of the guide plate is symmetrically provided with a sliding groove, a filtering membrane is movably arranged in the sliding groove and is movably connected with the guide plate through the sliding groove, the filtering membrane is always abutted against the guide plate, one end of the filtering membrane is provided with a pressing plate, one end of the pressing, the bottom movable mounting of movable rod drives actuating cylinder, and the bottom that drives actuating cylinder has the second base through bolt fixed mounting, and the second base passes through bolt and loading board fixed connection.

As a further scheme of the invention, the rotating plate is of a disc-shaped structure, a plurality of clamping grooves are formed in the rotating plate at equal intervals along the circumferential direction, clamping plates are clamped in the clamping grooves and are of an L-shaped structure, and the clamping plates are clamped and fixed with the rotating plate through the clamping grooves.

As a further scheme of the invention, one end of the clamping plate is provided with a mounting hole, a material taking groove is fixedly clamped in the clamping plate, and the material taking groove is of a hollow cylindrical structure.

As a further scheme of the invention, a plurality of clamping seats are symmetrically arranged at the bottom end of the supporting plate, the clamping seats are of a plate-shaped structure with a circular groove at the bottom end, the clamping seats are fixedly connected with the supporting plate, damping blocks are clamped and fixed at the bottom ends of the clamping seats through the grooves, and a baffle is arranged at one end of each clamping seat and fixedly connected with the supporting plate.

As a further scheme of the invention, a supporting rod is fixedly arranged at the bottom end of the damping block, the supporting rod is fixedly connected with the inner wall of the bottom end of the box body through a bolt, a buffer spring is arranged at the top end of the damping block, and the bottom end of the buffer spring is welded and fixed with the damping block.

As a further scheme of the invention, the box body is a square box body with a hollow interior, the top end of the box body is fixedly provided with a hinge through a bolt, one end of the hinge is fixedly connected with the cover plate through a bolt, the box body is movably connected with the cover plate through the hinge, and the bottom end of the box body is provided with a plurality of heat dissipation holes at equal intervals for accelerating heat dissipation.

As a further scheme of the invention, a movable groove is formed in one end of the box body, a push rod is movably mounted in the movable groove, a positioning rod is fixedly mounted at the top end of the push rod through a mounting hole, the positioning rod is movably connected with a mounting seat through a shaft hole, the mounting seat is fixedly connected with a cover plate through a bolt, a movable air cylinder is movably mounted at the bottom end of the push rod, and the bottom end of the movable air cylinder is fixedly connected with the inner wall of the bottom end of the box body through a bolt.

The working method of the protein purification device specifically comprises the following steps:

the method comprises the following steps that firstly, a centrifugal box is fixedly installed at the top end of a rotating plate through a bolt, the bottom end of the rotating plate is fixedly connected with a driving shaft through a bolt, a plurality of guide pipes are arranged at equal intervals at the bottom end of the centrifugal box along the circumferential direction, the guide pipes are communicated with the centrifugal box, a fixing plate is integrally connected to one ends of the guide pipes, a guide plate is fixedly installed at one end of the fixing plate, a filter hole is formed in the lower end of the guide plate, the size of the filter hole is matched with the aperture of each guide pipe, the guide plate is fixedly connected with the guide pipes through the filter holes in a clamping mode, and a filter membrane is movably connected with the guide;

a second step of fixedly mounting a first base on one end of the pressing plate through a bolt, movably mounting a positioning shaft on one end of the first base through a shaft hole, movably connecting the positioning shaft with a movable rod through a mounting hole, movably mounting a driving cylinder at the bottom end of the movable rod, fixedly mounting a second base at the bottom end of the driving cylinder through a bolt, and fixedly connecting the second base with the bearing plate through a bolt;

thirdly, putting the protein to be purified into the centrifugal box, starting the driving motor, driving the driving shaft to rotate by the driving motor, driving the rotating plate to rotate by the driving shaft, driving the centrifugal box to rotate by the rotating plate, transmitting the driving force to the centrifugal box by the rotating plate, and centrifugally separating the protein in the centrifugal box by the centrifugal box through centrifugal force generated by rotation;

and fourthly, when the process of protein filtration separation needs to be adjusted, starting the driving cylinder, driving the driving cylinder to drive the movable rod to move upwards, enabling the movable rod to push the pressing plate to move upwards through the first base, further enabling the pressing plate to move upwards to be attached to the guide plate, enabling the pressing plate to press the filtering membrane, and enabling the conduit to be separated from the storage box through the attachment of the pressing plate.

The invention has the beneficial effects that:

1. the pressing plate is arranged at one end of the filtering membrane, so that the pressing plate forms an acute included angle with the vertical direction in a non-working state, the first base is fixedly arranged at one end of the pressing plate through a bolt, the positioning shaft is movably arranged at one end of the first base through a shaft hole and is movably connected with the movable rod through a mounting hole, the driving cylinder is movably arranged at the bottom end of the movable rod, the second base is fixedly arranged at the bottom end of the driving cylinder through a bolt, and further when the process of filtering and separating the protein needs to be adjusted, the driving cylinder drives the movable rod to move upwards, so that the movable rod pushes the pressing plate to move upwards through the first base, and further the pressing plate moves upwards to be attached to the guide plate, so that the filtering membrane is pressed by the pressing plate, the guide pipe is separated from the storage box through the attachment of the pressing plate, the protein is not subjected to filtering operation any more, and then make the drive through driving actuating cylinder, can adjust the centrifugal separation degree of albumen in the centrifugation box to the adjustment gets into the protein purification degree that filter membrane got into in the case through the pipe and can adjust as required, thereby has improved the efficiency of protein purification greatly.

2. The storage box is internally and horizontally provided with a rotating plate, the top end of the rotating plate is fixedly provided with a centrifugal box through a bolt, the bottom end of the rotating plate is fixedly connected with a driving shaft through a bolt, the bottom end of the driving shaft penetrates through the storage box and extends downwards, the bottom end of the storage box is horizontally provided with a supporting plate, the bottom end of the driving shaft is rotatably connected with a guide sleeve through a bearing, the guide sleeve is fixedly connected with a bearing plate through a bolt, the driving shaft is rotatably connected with the supporting plate through a guide sleeve, the bearing plate is fixedly connected with the supporting plate through a bolt, the bottom end of the supporting plate is fixedly provided with a driving motor through a bolt, the driving motor is rotatably connected with the driving shaft, when the driving motor is started, the driving motor drives the driving shaft to rotate, so that the driving shaft drives the rotating plate to rotate, the efficiency of protein separation is improved.

Drawings

In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.

Fig. 1 is an overall installation schematic of the present invention.

Fig. 2 is a schematic view of the internal installation of the present invention.

Fig. 3 is a schematic view of the installation of the driving motor of the present invention.

Fig. 4 is a schematic view of the installation of the centrifuge cartridge of the present invention.

Fig. 5 is a schematic view of the installation of the catheter of the present invention.

Fig. 6 is a schematic view of the installation of the leader board of the present invention.

In the figure: 1. a box body; 2. a cover plate; 3. a hinge; 4. a movable groove; 5. a mounting seat; 6. a push rod; 7. a storage box; 8. a centrifuge cartridge; 9. heat dissipation holes; 10. a support bar; 11. a damping block; 12. a buffer spring; 13. a card holder; 14. a drive motor; 15. a support plate; 16. a guide sleeve; 17. a drive shaft; 18. a material taking groove; 19. a carrier plate; 20. a baffle plate; 21. a rotating plate; 22. clamping a plate; 23. a conduit; 24. a fixing plate; 25. leading the plate; 26. a filtration membrane; 27. a pin shaft; 28. a chute; 29. pressing a plate; 30. a first base; 31. positioning the shaft; 32. a movable rod; 33. a driving cylinder; 34. a second base; 35. a clamping groove.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, an apparatus for the tunable purification of proteins, comprising: the box body 1, the storage box 7, the centrifugal box 8, the support rod 10, the driving motor 14, the support plate 15, the driving shaft 17, the bearing plate 19, the rotating plate 21, the guide plate 25, the filtering membrane 26, the pressing plate 29, the movable rod 32 and the driving cylinder 33, wherein the storage box 7 is fixedly installed at the upper end of the box body 1, the storage box 7 is of a barrel-shaped structure, the rotating plate 21 is horizontally arranged in the storage box 7, the centrifugal box 8 is fixedly installed at the top end of the rotating plate 21 through a bolt, the bottom end of the rotating plate 21 is fixedly connected with the driving shaft 17 through a bolt, the bottom end of the driving shaft 17 penetrates through the storage box 7 to extend downwards, the support plate 15 is horizontally arranged at the bottom end of the storage box 7, the bottom end of the driving shaft 17 is rotatably connected with the guide sleeve 16 through a bearing, the guide sleeve 16 is fixedly connected with the bearing plate 19, there is driving motor 14 bottom of backup pad 15 through bolt fixed mounting, driving motor 14 rotates with drive shaft 17 to be connected, and then when starting driving motor 14, driving motor 14 drives drive shaft 17 and rotates, make drive shaft 17 drive rotor plate 21 and rotate, make rotor plate 21 drive centrifugal box 8 and rotate, thereby will drive power transmission to centrifugal box 8 through rotor plate 21, make centrifugal box 8 carry out centrifugal separation through rotatory production centrifugal force to the albumen in the box, the efficiency of albumen separation has been improved.

The bottom of centrifugal box 8 is provided with a plurality of pipes 23 along the circumferencial direction equidistant, a plurality of pipes 23 all communicate with centrifugal box 8, a plurality of pipes 23 are L venturi tube column structure, the one end body coupling of a plurality of pipes 23 has fixed plate 24, the one end fixed mounting of fixed plate 24 has guide plate 25, the lower extreme of guide plate 25 is provided with the filtration hole, the size of filtering the hole and the aperture looks adaptation of pipe 23, it is fixed with pipe 23 joint through filtering the hole to make guide plate 25, the one end of guide plate 25 passes through mounting hole fixed mounting has round pin axle 27, the one end of round pin axle 27 offsets with pipe 23, make round pin axle 27 for the fixed holding power that provides between pipe 23 and the guide plate 25, the one end symmetry of guide plate 25 is provided with spout 28, movable mounting has filtration membrane 26 in the spout 28, filtration membrane 26 passes through spout 28 and guide plate 25 swing joint, and filtration membrane 26 offsets with guide.

One end of the filtering membrane 26 is provided with a pressing plate 29, the pressing plate 29 is arranged at an acute included angle with the vertical direction under a non-working state, one end of the pressing plate 29 is fixedly provided with a first base 30 through a bolt, one end of the first base 30 is movably provided with a positioning shaft 31 through a shaft hole, the positioning shaft 31 is movably connected with a movable rod 32 through a mounting hole, the bottom end of the movable rod 32 is movably provided with a driving cylinder 33, the bottom end of the driving cylinder 33 is fixedly provided with a second base 34 through a bolt, the second base 34 is fixedly connected with the bearing plate 19 through a bolt, when the process of filtering and separating protein needs to be adjusted, the driving cylinder 33 is started, the driving cylinder 33 drives the movable rod 32 to move upwards, so that the movable rod 32 pushes the pressing plate 29 to move upwards through the first base 30, and further, the pressing plate 29 moves upwards to be attached to the guide plate 25, make pipe 23 cut off with the case 7 through the laminating of clamp plate 29 to make albumen no longer carry out filtering operation, thereby make albumen can be as required abundant centrifugal separation in centrifugal box 8, and then make the drive through driving actuating cylinder 33, can adjust the centrifugal separation degree of albumen in centrifugal box 8, thereby the adjustment gets into the albumen purification degree that filtration membrane 26 got into in the case 7 through pipe 23 and can adjust as required, thereby protein purification's efficiency has been improved greatly.

The rotor plate 21 is discoid structure, and the rotor plate 21 is provided with a plurality of draw-in grooves 35 along the equidistance of circumferencial direction, and the joint has cardboard 22 in a plurality of draw-in grooves 35, and cardboard 22 is L shape structure, and cardboard 22 passes through draw-in groove 35 and rotor plate 21 joint is fixed.

The one end of cardboard 22 is provided with the mounting hole, and the joint is fixed with the silo 18 in the cardboard 22, and the silo 18 is inside hollow cylindric structure, and the silo 18 is conveniently got from storage box 7.

The bottom symmetry of backup pad 15 is provided with a plurality of cassettes 13, and a plurality of cassettes 13 are provided with the platelike structure of circular recess for the bottom, a plurality of cassettes 13 all with backup pad 15 fixed connection, and a plurality of cassettes 13's bottom all is fixed with damping piece 11 through the recess joint, and a plurality of cassettes 13's one end all is provided with baffle 20, baffle 20 and backup pad 15 fixed connection.

The bottom fixed mounting of damping piece 11 has bracing piece 10, and bracing piece 10 passes through the bottom inner wall fixed connection of bolt and box 1, and buffer spring 12 is installed on the top of damping piece 11, and buffer spring 12's bottom and damping piece 11 welded fastening for buffer spring 12 can provide the buffering for backup pad 15.

Box 1 is inside hollow square box, and there is hinge 3 on the top of box 1 through bolt fixed mounting, and bolt and 2 fixed connection of apron are passed through to the one end of hinge 3, and box 1 passes through

hinge

3 and 2 swing joint of apron, and the equidistant louvre 9 that is used for with higher speed heat dissipation that is provided with in bottom of box 1.

One end of box 1 is provided with movable groove 4, movable mounting has push rod 6 in the movable groove 4, there is the locating lever on the top of push rod 6 through mounting hole fixed mounting, the locating lever passes through shaft hole and 5 swing joint of mount pad, mount pad 5 passes through bolt and apron 2 fixed connection, the bottom movable mounting of push rod 6 has the activity cylinder, the bottom of activity cylinder passes through bolt and box 1's bottom inner wall fixed connection, and then when the activity cylinder starts, the activity cylinder promotes push rod 6 and reciprocates, make push rod 6 drive mount pad 5 and remove, so that mount pad 5 promotes apron 2 and uses hinge 3 to rotate as the center, thereby make and to open or airtight between apron 2 and the box 1, with the convenience is sealed or open box 1 in purification process.

firstly, fixedly installing a centrifugal box 8 on the top end of a rotating plate 21 through bolts, fixedly connecting the bottom end of the rotating plate 21 with a driving shaft 17 through bolts, arranging a plurality of guide pipes 23 at equal intervals along the circumferential direction on the bottom end of the centrifugal box 8, enabling the guide pipes 23 to be communicated with the centrifugal box 8, integrally connecting a fixing plate 24 on one ends of the guide pipes 23, fixedly installing a guide plate 25 on one end of the fixing plate 24, arranging a filter hole at the lower end of the guide plate 25, enabling the size of the filter hole to be matched with the aperture of the guide pipe 23, clamping and fixing the guide plate 25 with the guide pipe 23 through the filter hole, movably connecting a filter membrane 26 with the guide plate 25 through a sliding groove 28, and enabling the filter membrane 26 to be always abutted against the guide;

secondly, fixedly installing a first base 30 at one end of a pressure plate 29 through a bolt, movably installing a positioning shaft 31 at one end of the first base 30 through a shaft hole, movably connecting the positioning shaft 31 with a movable rod 32 through an installation hole, movably installing a driving cylinder 33 at the bottom end of the movable rod 32, fixedly installing a second base 34 at the bottom end of the driving cylinder 33 through a bolt, and fixedly connecting the second base 34 with a bearing plate 19 through a bolt;

thirdly, putting the protein to be purified into the centrifugal box 8, starting the driving motor 14, driving the driving shaft 17 to rotate by the driving motor 14, enabling the driving shaft 17 to drive the rotating plate 21 to rotate, enabling the rotating plate 21 to drive the centrifugal box 8 to rotate, transmitting the driving force to the centrifugal box 8 through the rotating plate 21, and enabling the centrifugal box 8 to generate centrifugal force through rotation to carry out centrifugal separation on the protein in the box;

fourthly, when the protein filtering and separating process needs to be adjusted, the driving cylinder 33 is started, the driving cylinder 33 drives the movable rod 32 to move upwards, the movable rod 32 pushes the pressing plate 29 to move upwards through the first base 30, and then the pressing plate 29 moves upwards to be attached to the guide plate 25, so that the pressing plate 29 presses the filtering membrane 26, and the guide pipe 23 is separated from the storage box 7 through the attachment of the pressing plate 29.

When the device is used, the pressing plate 29 is arranged at one end of the filtering membrane 26, so that the pressing plate 29 forms an acute included angle with the vertical direction in a non-working state, the first base 30 is fixedly arranged at one end of the pressing plate 29 through a bolt, the positioning shaft 31 is movably arranged at one end of the first base 30 through a shaft hole, the positioning shaft 31 is movably connected with the movable rod 32 through a mounting hole, the driving cylinder 33 is movably arranged at the bottom end of the movable rod 32, the second base 34 is fixedly arranged at the bottom end of the driving cylinder 33 through a bolt, and when the process of filtering and separating protein needs to be adjusted, the driving cylinder 33 drives the movable rod 32 to move upwards, so that the movable rod 32 pushes the pressing plate 29 to move upwards through the first base 30, and further the pressing plate 29 moves upwards to be attached to the guide plate 25, so that the filtering membrane 26 is pressed by the pressing plate 29, and the guide pipe 23 is separated from, therefore, the protein is not filtered any more, so that the protein can be fully centrifugally separated in the centrifugal box 8 as required, the centrifugal separation degree of the protein in the centrifugal box 8 can be adjusted by driving the driving cylinder 33, and the protein purification degree of the protein entering the filtering membrane 26 through the conduit 23 into the storage box 7 can be adjusted as required, so that the protein purification efficiency is greatly improved; the storage box 7 is internally and horizontally provided with a rotating plate 21, the top end of the rotating plate 21 is fixedly provided with a centrifugal box 8 through a bolt, the bottom end of the rotating plate 21 is fixedly connected with a driving shaft 17 through a bolt, the bottom end of the driving shaft 17 penetrates through the storage box 7 and extends downwards, the bottom end of the storage box 7 is horizontally provided with a supporting plate 15, the bottom end of the driving shaft 17 is rotatably connected with a guide sleeve 16 through a bearing, the guide sleeve 16 is fixedly connected with a bearing plate 19 through a bolt, the driving shaft 17 is rotatably connected with the supporting plate 15 through the guide sleeve 16, the bearing plate 19 is fixedly connected with the supporting plate 15 through a bolt, the bottom end of the supporting plate 15 is fixedly provided with a driving motor 14 through a bolt, the driving motor 14 is rotatably connected with the driving shaft 17, and when the driving motor 14 is started, the driving shaft 14, therefore, the driving force is transmitted to the centrifugal box 8 through the rotating plate 21, so that the centrifugal box 8 generates centrifugal force through rotation to centrifugally separate protein in the box, and the protein separation efficiency is improved.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. An adjustable protein purification device, comprising: the centrifugal box comprises a box body (1), a storage box (7), a centrifugal box (8), a support rod (10), a driving motor (14), a support plate (15), a driving shaft (17), a bearing plate (19), a rotating plate (21), a guide plate (25), a filtering membrane (26), a pressing plate (29), a movable rod (32) and a driving cylinder (33), wherein the storage box (7) is fixedly arranged at the upper end of the box body (1), the storage box (7) is of a barrel-shaped structure, the rotating plate (21) is horizontally arranged in the storage box (7), the centrifugal box (8) is fixedly arranged at the top end of the rotating plate (21) through a bolt, the bottom end of the rotating plate (21) is fixedly connected with the driving shaft (17) through a bolt, the bottom end of the driving shaft (17) penetrates through the storage box (7) to extend downwards, the support plate (15) is horizontally arranged at the bottom end of the storage box, the guide sleeve (16) is fixedly connected with the bearing plate (19) through a bolt, the driving shaft (17) is rotatably connected with the supporting plate (15) through the guide sleeve (16), the bearing plate (19) is fixedly connected with the supporting plate (15) through a bolt, the bottom end of the supporting plate (15) is fixedly provided with a driving motor (14) through a bolt, and the driving motor (14) is rotatably connected with the driving shaft (17);

the bottom end of the centrifugal box (8) is provided with a plurality of guide pipes (23) at equal intervals along the circumferential direction, the guide pipes (23) are all communicated with the centrifugal box (8), the guide pipes (23) are of an L-shaped tubular structure, one ends of the guide pipes (23) are integrally connected with a fixing plate (24), one end of the fixing plate (24) is fixedly provided with a guide plate (25), the lower end of the guide plate (25) is provided with a filtering hole, the size of the filtering hole is matched with the aperture of the guide pipe (23), so that the guide plate (25) is fixedly clamped and connected with the guide pipes (23) through the filtering hole, one end of the guide plate (25) is fixedly provided with a pin shaft (27) through a mounting hole, one end of the pin shaft (27) is abutted against the guide pipes (23), one end of the guide plate (25) is symmetrically provided with sliding grooves (28), a filtering membrane (26) is movably mounted in the sliding grooves (28), and the, and filtration membrane (26) offsets with draw board (25) all the time, the one end of filtration membrane (26) is provided with clamp plate (29), there is first base (30) one end of clamp plate (29) through bolt fixed mounting, there is location axle (31) one end of first base (30) through shaft hole movable mounting, location axle (31) pass through mounting hole and movable rod (32) swing joint, the bottom movable mounting of movable rod (32) drives actuating cylinder (33), there is second base (34) bottom of driving actuating cylinder (33) through bolt fixed mounting, second base (34) pass through bolt and loading board (19) fixed connection.

2. The adjustable protein purifying device as claimed in claim 1, wherein the rotating plate (21) has a disc-shaped structure, the rotating plate (21) has a plurality of slots (35) disposed at equal intervals along a circumferential direction, the plurality of slots (35) are connected with the clamping plate (22) in a snap-fit manner, the clamping plate (22) has an L-shaped structure, and the clamping plate (22) is fixed to the rotating plate (21) in a snap-fit manner through the slots (35).

3. The adjustable protein purifying device according to claim 1, wherein a mounting hole is formed at one end of the clamping plate (22), the material taking groove (18) is fixed in the clamping plate (22) in a clamping manner, and the material taking groove (18) has a hollow cylindrical structure.

4. The adjustable protein purifying device according to claim 1, wherein a plurality of clamping seats (13) are symmetrically arranged at the bottom end of the supporting plate (15), the plurality of clamping seats (13) are plate-shaped structures with circular grooves at the bottom ends, the plurality of clamping seats (13) are fixedly connected with the supporting plate (15), the damping blocks (11) are fixedly clamped at the bottom ends of the plurality of clamping seats (13) through the grooves, the baffle plate (20) is arranged at one end of each of the plurality of clamping seats (13), and the baffle plate (20) is fixedly connected with the supporting plate (15).

5. The adjustable protein purifying device as claimed in claim 1, wherein a support rod (10) is fixedly mounted at the bottom end of the damping block (11), the support rod (10) is fixedly connected with the inner wall of the bottom end of the box body (1) through a bolt, a buffer spring (12) is mounted at the top end of the damping block (11), and the bottom end of the buffer spring (12) is welded and fixed with the damping block (11).

6. The adjustable protein purifying device as claimed in claim 1, wherein the box body (1) is a square box body with a hollow interior, the top end of the box body (1) is fixedly provided with a hinge (3) by a bolt, one end of the hinge (3) is fixedly connected with the cover plate (2) by a bolt, the box body (1) is movably connected with the cover plate (2) by the hinge (3), and the bottom end of the box body (1) is provided with a plurality of heat dissipation holes (9) at equal intervals for accelerating heat dissipation.

7. The adjustable protein purifying device according to claim 1, wherein a movable groove (4) is formed in one end of the box body (1), a push rod (6) is movably mounted in the movable groove (4), a positioning rod is fixedly mounted at the top end of the push rod (6) through a mounting hole and is movably connected with the mounting seat (5) through a shaft hole, the mounting seat (5) is fixedly connected with the cover plate (2) through a bolt, a movable cylinder is movably mounted at the bottom end of the push rod (6), and the bottom end of the movable cylinder is fixedly connected with the inner wall of the bottom end of the box body (1) through a bolt.

8. The adjustable protein purification device according to claim 1, wherein the working method of the protein purification device comprises the following steps:

firstly, a centrifugal box (8) is fixedly installed at the top end of a rotating plate (21) through a bolt, the bottom end of the rotating plate (21) is fixedly connected with a driving shaft (17) through a bolt, a plurality of guide pipes (23) are arranged at equal intervals along the circumferential direction at the bottom end of the centrifugal box (8), the guide pipes (23) are communicated with the centrifugal box (8), a fixing plate (24) is integrally connected with one ends of the guide pipes (23), a guide plate (25) is fixedly installed at one end of the fixing plate (24), a filtering hole is formed in the lower end of the guide plate (25), the size of the filtering hole is matched with the aperture of the guide pipe (23), the guide plate (25) is clamped and fixed with the guide pipe (23) through the filtering hole, and the filtering membrane (26) is movably connected with the guide plate (25) through a sliding groove (28), so that the filtering membrane (26) is always abutted against the guide plate;

secondly, fixedly mounting a first base (30) at one end of a pressing plate (29) through a bolt, movably mounting a positioning shaft (31) at one end of the first base (30) through a shaft hole, movably connecting the positioning shaft (31) with a movable rod (32) through a mounting hole, movably mounting a driving cylinder (33) at the bottom end of the movable rod (32), fixedly mounting a second base (34) at the bottom end of the driving cylinder (33) through a bolt, and fixedly connecting the second base (34) with a bearing plate (19) through a bolt;

thirdly, putting the protein to be purified into the centrifugal box (8), starting the driving motor (14), driving the driving shaft (17) to rotate by the driving motor (14), driving the rotating plate (21) to rotate by the driving shaft (17), driving the centrifugal box (8) to rotate by the rotating plate (21), and transmitting the driving force to the centrifugal box (8) by the rotating plate (21) so that the centrifugal box (8) generates centrifugal force through rotation to centrifugally separate the protein in the box;

fourthly, when the protein filtering and separating process needs to be adjusted, the driving cylinder (33) is started, the driving cylinder (33) drives the movable rod (32) to move upwards, the movable rod (32) pushes the pressing plate (29) to move upwards through the first base (30), and then the pressing plate (29) moves upwards to be attached to the guide plate (25), so that the pressing plate (29) presses the filtering membrane (26), and the guide pipe (23) is separated from the storage box (7) through the attachment of the pressing plate (29).