CN111374681A

CN111374681A - Detection device and detection method for animal anhedonia behaviors

Info

Publication number: CN111374681A
Application number: CN202010334534.3A
Authority: CN
Inventors: 周其冈; 许培金; 姜志欣; 夏天; 杨君言; 齐一泽; 霍子安; 谢紫嫣; 徐楚
Original assignee: Nanjing Medical University
Current assignee: Nanjing University; Nanjing Medical University
Priority date: 2020-04-24
Filing date: 2020-04-24
Publication date: 2020-07-07

Abstract

The application provides a detection device and a detection method for animal anhedonia missing behaviors, and relates to the technical field of animal behavior detection, wherein the detection device comprises a box body, a toy body, a camera shooting acquisition element and a master control controller; the box body is formed by enclosing a bottom plate and a plurality of side plates, the internal space of the box body forms a moving area of an animal to be detected, at least one toy body is arranged in the box body, a camera shooting acquisition element is further arranged above the internal space of the box body, a plurality of touch contact points are arranged on the surface of the toy body, and each touch contact point is respectively connected with a terminal circuit in the toy body; the master control controller is respectively connected with the camera shooting acquisition element and a terminal circuit inside each toy body, the toy bodies are connected with the box body in a plug-in mode, and the interface is unique, so that electrode potential is in contact with each other in a seamless mode. The detection device is simple in structure, convenient to operate when the anhedonia degree of the animal to be detected is evaluated, and then the detection efficiency of the depression behavior of anhedonia of the animal is improved.

Description

Detection device and detection method for animal anhedonia behaviors

Technical Field

The application relates to the technical field of animal behavior detection, in particular to a device and a method for detecting animal anhedonia behaviors.

Background

Depression is a mental disorder with persistent depressed mood as the main symptom. The anhedonia refers to the phenomenon that people cannot feel pleasure or experience capacity for pleasure is reduced, the phenomenon is one of main symptoms of depression patients, and the method for screening the depressed mice by utilizing the natural attribute indexes of the mice in a laboratory has great significance for clinical treatment of depression.

Sweet sugar water is preferred to be the natural attribute of the mouse, so the sugar water preference test can reflect the depressed core symptom of anhedonia, but the sugar water preference test needs more materials, the device structure is complex, the testing time is longer, the operation is complex, and the detection procedure is complicated.

Disclosure of Invention

The application provides a detection device and a detection method for animal anhedonia behaviors, the detection device is simple in structure and convenient to operate, and the detection efficiency of animal anhedonia depression behaviors is improved.

In a first aspect, an embodiment of the present application provides a device for detecting anhedonia behavior of an animal, where the device includes: the device comprises a box body, a toy body, a camera shooting acquisition element and a master control controller;

the box body is formed by enclosing a bottom plate and a plurality of side plates, wherein the inner space of the box body forms a moving area of the detected animal;

at least one toy body is arranged in the box body, and a camera shooting and collecting element is further arranged above the inner space of the box body;

the surface of the toy body is provided with a plurality of touch contact points, and each touch contact point is respectively connected with a terminal circuit in the toy body;

the main control controller is respectively connected with the camera shooting acquisition element and the terminal circuit in each toy body.

Preferably, a detachable cleaning plate is arranged at the inner bottom of the box body;

mounting holes corresponding to the toy bodies one to one are formed in the detachable cleaning plate, assembling holes corresponding to the mounting holes are formed in a box body bottom plate, and a circuit board is arranged on the box body bottom plate; each toy body penetrates through each mounting hole, is inserted into the corresponding assembly hole and is connected with the circuit board;

the circuit board is connected with the main control controller.

Preferably, a spring needle electrode male seat is arranged on the circuit board, a spring needle electrode female seat is arranged at the bottom of the toy body, a first magnet and a second magnet are respectively arranged at the corresponding positions of the bottom plate of the box body and the bottom of the toy body, and the spring needle electrode male seat is connected with the spring needle electrode female seat when the first magnet is coupled with the second magnet.

Preferably, the detection device further comprises a lighting lamp and a light adjusting switch, the lighting lamp is arranged above the inner space of the box body, and the lighting lamp and the light adjusting switch are respectively connected with the master control controller.

Preferably, the exterior of the toy body comprises a top toy area, a middle light sensing area and a lower touch sensing area;

wherein, top toy district includes the round bar, the round bar is used for making up arbitrary toy.

Preferably, the terminal circuit inside the toy body comprises a sensing touch circuit, a control light circuit and a touch data transmission circuit;

the perception touch circuit is connected with the touch data transmission circuit, and the touch data transmission circuit and the control light circuit are respectively connected with the main control controller;

the touch contact point is arranged on the lower touch sensing area of the toy body.

Preferably, an extension contact is further arranged on the middle light sensing area of the toy body, and the extension contact is connected with the lower touch sensing area of the toy body.

Preferably, the detection device further comprises a support, the support is arranged at the top of the box body, and the support is provided with two mounting positions which are respectively used for mounting the camera shooting collecting element and the illuminating lamp.

Preferably, the main control controller is provided with a functional touch operation interface, a power switch and a dimming panel.

In a second aspect, an embodiment of the present application provides a method for detecting anhedonia behaviors of animals, which uses the apparatus for detecting anhedonia behaviors of animals described in the first aspect to perform detection.

The embodiment of the application provides a device and a method for detecting animal anhedonia behaviors, wherein the device comprises a box body, a toy body, a camera shooting acquisition element and a master control controller; the box body is formed by enclosing a bottom plate and a plurality of side plates, wherein the inner space of the box body forms a moving area of an animal to be detected, at least one toy body is arranged in the box body, a camera shooting acquisition element is further arranged above the inner space of the box body, a plurality of touch contact points are arranged on the surface of the toy body, and each touch contact point is respectively connected with a terminal circuit in the toy body; the master control controller is respectively connected with the camera shooting acquisition element and the terminal circuit in each toy body. The detection device provided by the application is simple in structure, convenient to operate when the anhedonia degree of the animal to be detected is evaluated through the detection device, and then the detection efficiency of the depression behavior of anhedonia of the animal is improved.

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

Fig. 1 is a schematic structural diagram illustrating a device for detecting anhedonia behavior of an animal provided by an embodiment of the present application;

FIG. 2 is a schematic diagram illustrating a structure of a removable cleaning plate provided in an embodiment of the present application;

FIG. 3 is a schematic structural diagram of a bottom plate of a box provided by an embodiment of the present application;

FIG. 4 is a schematic diagram illustrating the connection of a toy body with a base of a case provided in an embodiment of the present application;

FIG. 5 shows a power and communications cabling schematic for a toy body as provided by an embodiment of the present application;

FIG. 6 is a schematic diagram illustrating a structure of a toy body provided by an embodiment of the present application;

FIG. 7 illustrates a bottom schematic view of a toy body provided by an embodiment of the present application;

FIG. 8 is a schematic diagram illustrating an internal structure of a toy body provided by an embodiment of the present application;

fig. 9 is a schematic structural diagram of another detection device for animal anhedonia behavior provided by the embodiment of the present application;

FIG. 10 shows the results of an animal depressive behavior experiment conducted in Experimental example 1 of the examples of the present application;

fig. 11 shows the results of an animal depression behavior experiment performed in experimental example 2 of the present application example;

fig. 12 shows the results of an animal depression behavior experiment conducted in test example 3 of the present application example;

fig. 13 shows the results of an animal depression behavior experiment performed in test example 4 of the example of the present application.

Reference numerals: 10-a detection device; 11-a box body; 111-removable cleaning plates; 112-mounting holes; 113-carrying handle; 114-a box floor; 115-assembly holes; 12-a toy body; 121-round bar; 122-middle light sensing zone; 123-lower touch sensing zone; 124-fixed column; 125-pogo pin electrode female seat; 126-a second magnet; 1211-LED lamp; 1212-power, communication connection; 1213-sense lead tab; 1214-lead and metal wall contact points; 1215-a strut; 13-a camera capture element; 14-a master controller; 15-bracket.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.

In the description of the embodiments of the present application, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention are usually placed in when used, and are only used for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements indicated must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or pendant, but rather may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the embodiments of the present application, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Exploring novelty and environment is an innate attribute of mice, depressed mice have reduced interest and behavior in exploring novelty, which can reflect the depressed mood of the mice. Toys with different colors and different shapes are used as media for stimulating the interest of the mouse, and meanwhile, the method is also a mode for judging whether the interest of the mouse is lost, so that the depression behavior of the mouse with anhedonia can be effectively detected, and a more accurate detection device and a detection method for the anhedonia behavior of the animal are provided for the depression behavior of the detected mouse.

In a first aspect, the present application provides a device for detecting anhedonia behavior of an animal, as shown in fig. 1, a detection device 10 includes: the device comprises a box body 11, a toy body 12, a camera shooting and collecting element 13 and a master control controller 14;

the box body 11 is formed by enclosing a bottom plate and a plurality of side plates, wherein the inner space of the box body 11 forms a moving area of a detected animal, at least one toy body 12 is arranged in the box body 11, a camera shooting acquisition element 13 is further arranged above the inner space of the box body 11, a plurality of touch contact points are arranged on the surface of the toy body 12, each touch contact point is respectively connected with a terminal circuit inside the toy body 12, and the master control controller 14 is respectively connected with the camera shooting acquisition element 13 and the terminal circuit inside each toy body 12.

In the embodiment of the application, the box body 11 is formed by surrounding a bottom plate and four side plates, five surfaces of the box body are closed, the upper part of the box body is opened, and the inner space of the box body 11 forms the moving area of the detected animal so as to accommodate the detected animal and allow the detected animal to move.

At least one toy body 12 is arranged in the box body 11, 16 toy bodies 12 are uniformly arranged in the box body 11 in order to improve the probability that a detected animal touches the toy bodies 12, each toy body 12 is cylindrical, the fixing mode of the toy bodies 12 is a direct insertion type, and the player bodies 12 can be directly inserted and pulled out when being replaced every time.

The camera pickup element 13 is a camera, and the camera in the embodiment of the present application includes 500-.

A detachable cleaning plate 111 is arranged at the inner bottom of the box body 11, and mounting holes 112 corresponding to the toy bodies 12 one by one are formed in the detachable cleaning plate 111, as shown in fig. 2; the box body bottom plate 114 is provided with an assembly hole 115 corresponding to each mounting hole 112, and the box body bottom plate 114 is provided with a circuit board, as shown in fig. 3; each toy body 12 passes through each mounting hole 112, is inserted into the corresponding assembly hole 115 and is connected with the circuit board; the circuit board is connected to a master controller 14 as shown in fig. 4.

Specifically, can dismantle and be equipped with the mounting hole 112 with the toy body 12 one-to-one on the cleaning plate 111, be equipped with on the side of can dismantling cleaning plate 111 and carry handle 113, make things convenient for the experimenter to dismantle cleaning plate 111 from the inside dismantlement of box 11, and then be convenient for can dismantle cleaning plate 111 and wash, make the experiment go on fast.

The circuit board on the box bottom board 114 is connected with 16 toy bodies 12, wherein the number of the toy bodies 12 is adjustable, and the wires among the toy bodies 12 are all connected in parallel and connected with the master controller 14 through a bus, as shown in fig. 5.

In the embodiment of the present application, as a preferred embodiment, a pogo pin electrode male socket (not shown in the drawings) is provided on the circuit board, a pogo pin electrode female socket 125 is provided at the bottom of the toy body 12, a first magnet (not shown in the drawings) and a second magnet 126 (shown in fig. 6 and 7) are provided at positions corresponding to the bottom plate 114 of the case body and the bottom of the toy body 12, respectively, and the pogo pin electrode male socket and the pogo pin electrode female socket 125 are connected when the first magnet is coupled with the second magnet 126.

In the embodiment of the present application, when the case 11 is assembled with the toy body 12, the toy body 12 needs to be aligned with the bottom plate 114 of the case and put on the bottom plate lightly. Because the bottom of the toy body 12 and the box body bottom plate 114 are respectively provided with the first magnet and the second magnet 126 with the diameters of 8mm, the spring pin electrode female seat 125 is arranged at the bottom of the toy body 12, and the spring pin electrode male seat is arranged on the circuit board of the box body bottom plate 114, after the upper part and the lower part are aligned, the electrodes are tightly combined together due to the strong attraction of the magnetic steel of the first magnet and the second magnet 126.

Furthermore, the bottom of the toy body 12 is provided with fixing columns which are distributed at intervals around the bottom of the toy body for one circle, and a spring needle electrode female seat is arranged at the center of the bottom of the toy body 12, wherein in order to ensure that the problem of wrong insertion does not exist when the toy body 12 is inserted into the box bottom plate 114, the fixing columns are arranged in a regular shape, but the gap between every two adjacent fixing columns is irregular, or the fixing columns are arranged in an irregular shape, but the gap between every two adjacent fixing columns is regular, so that when the toy body 12 is inserted into the box bottom plate 114, the fixing columns play a certain guiding role, and the problem that the circuit at the bottom of the toy body is damaged due to wrong insertion of the toy body is effectively. Specifically, as shown in fig. 7, 3 fixing posts 124 are arranged at the bottom of the toy body 12, an included angle formed between two ends of each fixing post 124 and the center of the bottom of the cylindrical toy body is 60 °, and meanwhile, an included angle formed between a gap interval between two adjacent fixing posts 124 and the center of the bottom of the cylindrical toy body is respectively 30 °, 60 ° and 90 °; similarly, the shape of the assembly hole 115 on the box bottom plate 114 is matched with the fixing column 124 at the bottom of the toy body 12, so that when the toy body 12 is inserted into the box bottom plate 114, only the fixing column 124 at the bottom of the toy body 12 needs to be inserted into the assembly hole 115 on the box bottom plate 114, the position and the direction of an interface are unique, and the problem that the cylindrical toy body is mistakenly inserted to cause circuit damage can be avoided, wherein the first magnet on the bottom of the toy body 12 is coupled with the second magnet 126 on the box bottom plate 114, and the attraction is tight, so that the spring pin electrode male seat of the circuit board on the box bottom plate 114 is fully contacted with the spring pin electrode female seat at the bottom of the toy body 12, and further, all the leads are conducted.

The single toy body 12 and the box body bottom plate 114 are in a magnetic type, so that the toy body 12 and the box body bottom plate 114 can be conveniently and quickly detached after an experiment is finished, and the connection between the toy body 12 and the box body bottom plate 114 adopts a modern spring pin terminal, namely, a male head and a female head are spring contacts.

In the embodiment of the present application, as a preferred embodiment, the detecting device 10 further includes an illuminating lamp (not shown) and a light adjusting switch (not shown), the illuminating lamp is disposed above the inner space of the box 11, and the illuminating lamp and the light adjusting switch are respectively connected to the main controller 14.

As shown in FIG. 6, the exterior of the toy body 12 includes a top toy area, a middle light sensing area 122 and a lower touch sensing area 123; wherein, top toy district includes pole 121, and pole 121 is used for making up arbitrary toy, and specifically, pole 121 can be dismantled, and metal can be chooseed for use to pole 121's material.

Toys with different colors and shapes can be replaced in the top toy area, the toy is sleeved on the round rod 121, and only the toy needs to be pulled out of the round rod 121 during replacement, and the replacement toy is inserted into the round rod 121; the fixing mode of the toy body 12 adopts a direct insertion mode, the bottom of the toy body 12 is provided with a fixing column 124, and the fixing column 124 of the toy body is inserted into the assembly hole 115 of the box body bottom plate 114 and can be directly inserted and pulled out when replaced each time. The middle light induction area 122 is a colorful luminous area with variable colors, can emit colorful colors, is made of semitransparent ground glass or other materials, is covered with a wire mesh on the outer layer of the surface of the toy body 12, and is in full contact with the lower touch induction area 123, so that the induction touch detection area of the mouse is increased, and the induction area is enlarged; the touch induction zone at the lower part adopts a capacitance sensing mode, is an electrophysiological induction zone touched by the mouse and is made of a metal material on the surface.

Further, the toys can be stacked on the rod 121, and the shapes can be diversified. In the embodiment of the application, the top toy area comprises two toys with different shapes, the toy shapes can comprise a sphere, a square, a cone, an irregular shape and the like, the colors of the toys are random, and the search desire of the mouse can be further increased by the aid of a richer attraction form. Specifically, all the toy bodies 12 are regular and uniform in shape, are made of organic glass and stainless steel, can be stably connected with the box body bottom plate 114, are suitable for the mouse in height, and avoid the problem that the experimental result is influenced by the fact that the mouse climbs the top end of the toy body 12 to stay, and the number of touch times is reduced in the effective time; meanwhile, the toy bodies 12 are different in color and have stickers of different shapes, a certain attraction is provided for the mouse, the exploration desire of a normal mouse is strong, and the frequency of touching the toy bodies 12 is large, so that the anhedonia behavior of the mouse can be tested by the frequency of touching the toy bodies 12 by the detected mouse. In the embodiment of the present application, the length, width and height of the internal activity space of the box body 11 are respectively: 610, 350mm, 16 built-in cylindrical toy bodies 12 are tightly combined with the box body bottom plate 114 and are convenient to disassemble, the distance between the toy bodies 12 is fixed to be 150mm, the toy bodies are uniformly distributed and are proper in distance, the number and the distance of the toy bodies 12 can be properly increased and decreased according to the length of a detected mouse, the touch of other body parts of the detected mouse on the toy bodies 12 is reduced, relevant interference factors are eliminated, and experimental errors are reduced.

In the embodiment of the present invention, the middle light-sensing region 122 is made of translucent frosted glass, but not limited thereto, and the inner LED 1211 can emit a plurality of colors. Further, the embodiments of the present application can attract the attention of the mouse by the colored LED lights emitted from the variously shaped toys in the top toy area and the middle light sensing area 122.

The terminal circuit inside the toy body 12 comprises a sensing touch circuit, a control light circuit and a touch data transmission circuit;

the perception touch circuit is connected with the touch data transmission circuit, and the touch data transmission circuit and the control light circuit are respectively connected with the main control controller 14;

wherein, the touch contact point is arranged on the lower touch sensing area 123 of the toy body 12.

Specifically, data of the sensing touch circuit, the control light circuit and the touch data transmission circuit are collected and then transmitted to the master control controller 14, and data processing is performed by the master control controller 14, wherein a terminal circuit inside the toy body 12 reports data to the master control controller 14 through a CAN bus, and CAN bus communication CAN guarantee real-time data reporting, so that complex to simple wiring is achieved, and wiring is simplified.

In addition, the connection mode between the toy body 12 and the master control 14 can also adopt wireless connection, so that the wiring can be saved. The specific implementation mode is as follows: a power supply is arranged in the toy body and used for supplying power to an internal circuit of the toy body 12, and a signal receiving device is arranged at the same time, wherein the signal receiving device can be a Bluetooth module or an infrared module, so that data transmission between the toy body 12 and the master control controller 14 can be realized only by connecting the master control controller 14 with the signal receiving device in the toy body 12.

As shown in fig. 8, an LED 1211 is disposed in the middle light sensing area 122 of the toy body 12, the LED 1211 is supported in the middle light sensing area 122 of the toy body 12 through a support 1215, a power supply and a communication connector 1212 are disposed in the toy body 12, the power supply and the communication connector 1212 disposed inside the toy body 12 are connected to the power supply and the communication connector 1212 disposed at the bottom of the case 11, and a sensing lead connector 1213 disposed inside the toy body 12 is connected to a lead wire and a metal wall contact point 1214 disposed outside the toy body 12, so that the touch sensing area 123 disposed at the lower portion of the toy body 12 can sense the touch of the mouse, and transmit the number of the touches to the main controller 14.

In the embodiment of the present application, as a preferred embodiment, an extension contact is further disposed on the middle light sensing area 122 of the toy body 12, and the extension contact is connected to the lower touch sensing area 123 of the toy body 12.

In the embodiment of the application, the extension contact is arranged on the middle light sensing area 122 of the toy body 12, and the extension contact is connected with the lower touch sensing area 123 of the toy body 12, so that the sensing touch area of the detected animal can be enlarged, and the touch sensing area is enlarged. Because the surface of the lower touch sensing area 123 is made of metal, the arrangement mode of the extension contacts is to cover the metal wire mesh on the outer layer of the surface of the middle light sensing area 122 of the toy body 12.

As shown in fig. 1, the detecting device 10 further includes a bracket 15, the bracket 15 is disposed on the top of the box 11, and two mounting positions are disposed on the bracket 15 and are respectively used for mounting the camera shooting collecting element 13 and the illuminating lamp.

Specifically, the illumination lamp is installed on the top of the box 11 of the detection device 10 to assist the illumination of the internal environment of the box 11, and the brightness of the illumination lamp is adjusted by the dimming panel on the main control 14. The camera is installed at the top of box 11 of detection device 10, overlooks the active area of detection device 10, and main control controller 14 is connected to the camera, and the video recording is started or stopped by main control controller 14 control camera, and the video is preserved on main control controller 14's storage card, and experiment operator and user can derive the video file through the USB flash disk. This detection device 10 can be upgraded for the high in the clouds storage experiment data, can pass through the high in the clouds in real time and filter interference data through the camera during the experiment.

In the embodiment of the present application, as a preferred embodiment, the main controller 14 is provided with a functional touch operation interface, a power switch and a dimming panel.

In this embodiment, the main controller 14 is also referred to as a host, is a control center of the detection device 10, and is configured with a 7-inch capacitive touch screen, and is configured to receive real-time monitoring data of the 16 toy bodies 12 and video data of the camera acquisition element 13, and control display colors of the LED lamps 1211 inside the toy bodies 12.

The dimming panel includes switches for controlling the LED lamps 1211, and may also adjust the display color, light intensity, conversion mode, etc. of the LED lamps 1211.

In the embodiment of the present application, the master controller 14 includes a screen (7 inch capacitive screen, 1024 × 600(mm)), a processor (4-core platform-level rail smart processor), a RAM (2G), an eMMC FLASH (32G), a CAN bus, a USB Camera, an operating system (andriod7.1), and a power supply (8-40V), wherein the external size is 210 × 150 × 60 (mm).

Specifically, the functional touch operation interface of the main control controller 14 is a full chinese interface, and is automatically started when powered on, and the functions mainly include: setting experiment time till the experiment is automatically finished; the LED lamp color conversion mode inside the toy body comprises random conversion, sequential color conversion, conversion interval time and brightness adjustment; modifying the login password; managing storage space, checking total amount and allowance, and emptying data; detecting an online toy body; monitoring and displaying real-time touch data in real time; counting and analyzing the touch indexes; querying and viewing historical data; exporting historical data, reports and videos; and controlling the start and stop of the experiment.

The detection device 10 adopts 220V alternating current power supply, the total power is less than 50W, a three-pin plug is adopted to be fully grounded, a power switch power supply is used inside the detection device to perform direct current conversion, stable voltage and current values are ensured to be output, and alternating current power supply control and circuit protection can be directly improved through an air switch. The main control controller 14 is installed on the side or above the detection device 10, and the main board USB is led out to the device case 11 through a connection line.

Preferably, the box body 11, the toy body 12 and the like are made of non-reflective materials, so that the condition that the video pictures of the camera are influenced due to the fact that light irradiates the box body 11 and the toy body 12 to reflect light in a large area can be avoided; in addition, in order to better distinguish the detected animal from the box body 11 or the toy body 12, the box body 11 does not adopt black or white, because most of animals are black or white in appearance, in the embodiment of the application, the detected animal is a mouse, and further, the box body 11 adopts a 6mm blue single-side frosted acrylic plate or a 6mm double-side frosted gray PVC plate, so that the box body is suitable for both a white mouse and a black mouse to perform experiments.

The above description is only a preferred embodiment of the present application, and it will be apparent to those skilled in the art that various modifications and variations can be made in the present application, such as modifications of the size of the apparatus, modifications of the material and the shape of the box body, modifications of the material and the shape of the toy and the spacing thereof, modifications of the detection indexes of the apparatus, and the like. Any modification, equivalent replacement, improvement and the like made within the principle of the embodiments of the present application shall be included in the protection scope of the embodiments of the present application. As shown in FIG. 9, in order to facilitate the operation of the experimenter when operating the host, the host operating interface is inclined at an angle of 60-70 degrees with the ground, so that the experimenter can operate by bending over, and the problems that the operation is inconvenient because the original design needs to be squat are solved.

The detection device of animal's emotional disappearance action that this application embodiment provided adopts automatic controlling part real-time detection mouse whether touch to play the body, gathers and analysis data in real time through Artificial Intelligence (AI) technique, and the experimenter can set up the experiment parameter on the intelligent control screen, can gather data when beginning the experiment, also can record a video in real time, and the device simple structure can effectively assess the depressed core symptom (the anhedonia condition) of mouse. The detection device of this application is irrelevant with the size and the physical stamina difference of mouse, can get rid of the influence of these factors, and the anhedonia degree of more direct measurement mouse. The detection device has the advantages of simple structure, less influence by external environment, the mobility of the detected mouse, the appetite of the detected mouse and the like, better firmness, low cost and capability of being manufactured into different sizes according to actual conditions. Simple structure, convenient operation when assessing the anhedonia degree of waiting to detect the animal through this detection device, and then improved the detection efficiency of animal anhedonia's depressive behavior.

The detected animals can be divided into three groups, namely normal detected animals, detected animals with depression symptoms and detected animals after being given with antidepressant, the detected animals with depression symptoms have less touch frequency on a toy body due to lack of interest, and the frequency of touching the toy body by the detected animals is increased because the lack of interest symptoms are improved after being given with antidepressant (positive medicine).

The detection method comprises the following steps: in the first stage, a plurality of detected animals are respectively placed at the central positions of the detection devices, and the times of touching the toy by the detected animals in the movable area within a fixed time period are recorded; in the second stage, after an antidepressant is provided for a plurality of detected animals for 1 to 2 hours, each detected animal is placed at the center position of the detection device, and the times of touching the toy by the detected animals in the movable area in a fixed time period are recorded.

In the embodiment of the application, the toy body can emit colored LED light, and toys with different shapes above the toy body can be changed to attract the attention of a mouse; the fixed time period is six minutes, and the number of times the toy is touched by the nose or forelimb in the six regions of the animal to be tested is recorded, and if the drug is needed, the test is carried out 1 to 2 hours after the drug is applied. Besides, the animals to be tested in the same test group can be placed in the same position on the test device, and the position is not limited to the central position of the test device.

Test example 1: and (3) detecting the behavior of the anti-depression drug in the mouse after acute administration.

The depression behavior of a male C57 mouse is detected by an acute intraperitoneal injection mode by using a device for detecting animal anhedonia behaviors.

The experimental animals were divided into three groups: control group, ketamine group (i.p., 10mg/kg) and fluoxetine group (i.p., 10 mg/kg). The detection device for the animal anhedonia behaviors is adopted to detect the animal depression behaviors. As shown in fig. 10, the ordinate represents the number of times the mice touched the toy body, the abscissa represents the group, which is divided into the control group, the ketamine group and the fluoxetine group, and one dot in the figure represents the number of times one mouse touched the toy body, the number of times the mice touched the toy body is significantly increased (P < 0.001) compared with the control group, and the number of times the mice touched the toy body is also significantly increased (P < 0.05) compared with the fluoxetine group. The ethological results in the graph a in fig. 10 show that after acute intraperitoneal injection of two antidepressants, namely ketamine and fluoxetine, the excitability of the mice is increased, and the frequency of playing the toy body by touching is obviously increased, namely the exploration desire and the pleasure of the toy body are enhanced. The detection device for the animal anhedonia behaviors can well reflect the depression behaviors of mice, and is also suitable for long-term chronic administration of ketamine (intraperitoneal injection, 10mg/kg) and fluoxetine (intraperitoneal injection, 10 mg/kg). The same dose was given daily for one month and the same test method was used to conduct the behavioural test on three groups of mice after the corresponding groups had been injected with saline, ketamine and fluoxetine using the animal's anorgasmia behaviour test device. The results of the b-plot in fig. 10 show that after chronic administration for a long period of time, mice in the ketamine group had a significant increase in the number of mice touched with the body (P < 0.001), and mice in the fluoxetine group had a similar significant increase in the number of mice touched with the body (P < 0.001). The detection results of the graph a in fig. 10 and the graph b in fig. 10 show again that the detection device for animal anhedonia behaviors is suitable for detecting the anhedonia difference generated by mice after long-term and acute antidepressant administration, and can be used as a sensitive behavioral detection device for the development of antidepressant treatment drugs.

Test example 2: and (3) detecting the behavior of the anti-depression drug in the mouse after chronic administration.

A male C57BL/6Ntac mouse is modeled by adopting a classical depression model, namely a chronic mild unpredictable stress depression model (CUMS), which is widely used at present, and depression behaviors of the male C57BL/6Ntac mouse are detected by utilizing a device for detecting animal anhedonia behaviors. The experimental animals were divided into three groups, namely: control group, fluoxetine (i.p., 10mg/kg) + CUMS group and CUMS control group. As shown in fig. 11, the ordinate represents the number of times the mice touched the toy body, the abscissa represents the group, and the group is divided into a control group, a fluoxetine + CUMS group, and a CUMS control group, and one dot in the figure represents the number of times one mouse touched the toy body, and administration was continued for 31 days in the sixth to ninth weeks of molding while the control group mice were injected with saline intraperitoneally. Then, on the 31 st day of administration, the three groups of experimental groups are subjected to animal depression behavior detection by using the animal anhedonia behavior detection device. The experimental results in panel a of FIG. 11 show that the number of times of mice in fluoxetine (i.p., 10mg/kg) + CUMS group touched with the toy body was significantly increased (P < 0.0001) compared to the CUMS control group; the number of mice in fluoxetine (i.p., 10mg/kg) + CUMS group touched the body was significantly increased (P < 0.0001) compared to the control group in panel b of fig. 11. Further shows that the animal anhedonia behavior detection device is suitable for detecting anhedonia difference generated by a depression model, namely a chronic mild unpredictable stress depression model (CUMS) mouse, and an antidepressant, and can be used as a sensitive behavioural detection device for research and development of antidepressant treatment drugs.

Test example 3: and detecting the depression behavior of the TERT gene knockout mouse.

TERT (telomerase catalytic subunit) gene knockout mice have obvious depression symptoms, and animal depression behavior detection is carried out on the TERT gene knockout mice by using an animal anhedonia behavior detection device.

The experimental animals were divided into two groups, namely: as shown in FIG. 12, the ordinate of the control group and the TERT gene knockout mouse represents the frequency of touching the mouse body, the abscissa of the control group and the TERT gene knockout mouse represents the group, the control group and the TERT gene knockout mouse are divided into a control group and a TERT gene knockout group, one circular dot in the figure represents the frequency of touching the mouse body, and the detection device for the animal anhedonia behavior is adopted to detect the animal depression behavior. The experimental results show that the number of times of touching the toy body of the TERT gene knockout mouse is remarkably reduced (P is less than 0.05) compared with the control group. The experiment of figure 12 shows that the detection device for animal anhedonia behavior is suitable for detecting anhedonia behavior of depressed mice with gene knockout, and can be used as a sensitive ethology detection device for research and development of antidepressant drugs.

Test example 4: the sensitivity of the detection device for detecting the anhedonia behaviors of the animals in the detection of the anxiety behaviors of the mice.

The depression behavior of a male C57 mouse is detected by using a device for detecting the animal anhedonia behavior in an acute intraperitoneal injection mode.

The experimental animals were divided into two groups: in a control group, a diazepam group (intraperitoneal injection, 10mg/kg), the animal depression behavior is detected by adopting the detection device for the animal anhedonia behavior. As shown in FIG. 13, the ordinate represents the number of times the mouse touched the toy body, the abscissa represents the group, and the group is divided into the control group and the diazepam group, and one dot in the figure represents the number of times one mouse touched the toy body, and the anxiolytic dose (0.5mg/kg) of diazepam was used in the present example. The experimental results in fig. 13 show that the mice in the diazepam group had more stable frequency of touching the toy bodies, less intra-group variation, and no statistical difference (P > 0.05) from the control group, compared to the control group. The test example excludes the possibility that the number of times of touching the toy body is increased due to anxiety and dysphoria of the mouse, and further shows the sensitivity and the specificity of the detection device for the anhedonia behavior of the animal for detecting the depression behavior of the mouse.

According to the analysis of the test examples 1 to 4, the detection device for the animal anhedonia behaviors provided by the embodiment of the application can better detect the depression behaviors of the mouse, and further, the detection method for the animal anhedonia behaviors provided by the application can effectively identify the difference of the acute desire to explore toys among antidepressants, normal mice and various depressed mice, has higher clinical application value in principle, can effectively solve the problem that the behaviors of anxiety and depression of the mouse cannot be distinguished in a strange environment ingestion test, saves the test time and space, can obtain the behavioural data of one mouse only in six minutes, and is convenient for an experimenter to operate.

In addition, sweet sugar water is also the natural attribute of mice, so the sugar water preference test can reflect the depressed core symptom of anhedonia, but the sugar water preference test has the following defects: (ii) affected by taste in mice; the testing time is long, and at least 3 days are needed; the sensitivity is low, and the improvement of the mouse emotion cannot be reflected under physiological conditions; the detection procedure is complicated; the detection cost is relatively high. In order to overcome the defects, the device for detecting the animal anhedonia behaviors, which is provided by the embodiment of the application, has a simple structure and is convenient to operate, is irrelevant to the body type and physical energy difference of a mouse, can eliminate the influence of the factors, and can more directly measure the anhedonia degree of the mouse; the influence of external environment, the self activity of the detected mouse, the appetite of the detected mouse and the like is small, the ethological data of the mouse can be obtained in six minutes, the consumed time is short, interference factors can be eliminated, the depression behavior of the mouse with anhedonia can be effectively detected, and a more accurate detection device and a detection method are provided for the depression behavior of the mouse.

Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present application, and are used for illustrating the technical solutions of the present application, but not limiting the same, and the scope of the present application is not limited thereto, and although the present application is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope disclosed in the present application; such modifications, changes or substitutions do not depart from the spirit and scope of the exemplary embodiments of the present application, and are intended to be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. A detection apparatus for animal anorgasmia behavior, the detection apparatus comprising: the device comprises a box body, a toy body, a camera shooting acquisition element and a master control controller;

2. The detection device according to claim 1, wherein a detachable cleaning plate is arranged at the inner bottom of the box body;

the circuit board is connected with the main control controller.

3. The detection device as claimed in claim 2, wherein a pogo pin electrode male socket is provided on the circuit board, a pogo pin electrode female socket is provided on the bottom of the toy body, a first magnet and a second magnet are provided at corresponding positions on the bottom plate of the case body and the bottom of the toy body, respectively, and the pogo pin electrode male socket and the pogo pin electrode female socket are connected when the first magnet and the second magnet are coupled.

4. The detecting device according to claim 1, further comprising a lighting lamp and a light adjusting switch, wherein the lighting lamp is disposed above the inner space of the box body, and the lighting lamp and the light adjusting switch are respectively connected to the main control controller.

5. The detection apparatus as claimed in claim 4, wherein the exterior of the toy body comprises a top toy zone, a middle light sensing zone and a lower touch sensing zone;

6. The detection device according to claim 5, wherein the internal terminal circuit of the toy body comprises a sensing touch circuit, a control light circuit and a touch data transmission circuit;

7. The detection device according to claim 5, wherein an extension contact is further arranged on the middle light sensing area of the toy body, and the extension contact is connected with the lower touch sensing area of the toy body.

8. The detection device according to claim 4, further comprising a support, wherein the support is disposed on the top of the box body, and two mounting positions are disposed on the support and are respectively used for mounting the camera shooting and collecting element and the illuminating lamp.

9. The detection device according to claim 1, wherein the main controller is provided with a functional touch operation interface, a power switch and a dimming panel.

10. A method for detecting anhedonia behaviors of animals, characterized in that the method is performed by using the device for detecting anhedonia behaviors of animals according to any one of claims 1 to 9.