CN111373898A - Method for germinating wheat stripe rust fungus primary host seed into seedling - Google Patents
Method for germinating wheat stripe rust fungus primary host seed into seedling Download PDFInfo
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- CN111373898A CN111373898A CN202010192776.3A CN202010192776A CN111373898A CN 111373898 A CN111373898 A CN 111373898A CN 202010192776 A CN202010192776 A CN 202010192776A CN 111373898 A CN111373898 A CN 111373898A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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Abstract
The invention discloses a method for germinating wheat stripe rust fungus primary host seeds into seedlings, which is characterized by comprising the following steps: the method comprises the following steps: collecting and storing: collecting mature seeds and storing the seeds at low indoor temperature; seed pretreatment: washing pulp remained on the neutron surface by using clean water, and cleaning the pulp by using clean water after disinfection treatment; pretreatment of the container: putting W-shaped folded filter paper or gauze at the bottom of the container; seed transferring: spreading the seeds in a container, pouring water for seed soaking treatment, and covering a container cover; germination: placing the container in an incubator for germination at 16-20 ℃, pouring off excessive water in the container at intervals of 24h, and continuing culturing until neutrons germinate; seedling formation: and (5) transferring the germinated seeds in the step (5) to a culture pot, and culturing seedlings in a growth chamber. The invention establishes the method suitable for the puccinia striiformis to transfer the main host seeds to germinate and grow seedlings, simplifies the seed germination treatment process, accelerates the seed germination and seedling raising speed, and improves the seed germination efficiency and the research efficiency.
Description
Technical Field
The invention relates to the field of seed germination, in particular to a method for germinating wheat stripe rust by transferring a main host seed to a seedling.
Background
Wheat stripe rust caused by stripe rust is one of the most important wheat diseases in the world, the wheat yield is seriously influenced, and the production safety of wheat is greatly threatened. In recent years, researches at home and abroad find that the berberis thunbergii and the mahonia are the primary hosts of the puccinia striiformis. By identifying the transgenic hosts of the yellow rust of wheat, namely the Berberis thunbergii and the Mahonia fortunei, the high population genetic diversity and the toxicity variation mechanism of the yellow rust of wheat can be further disclosed, and the method has very important theoretical and practical significance for perfecting the control strategy of the yellow rust of wheat.
The resources of the berberis and the Chinese mahonia are extremely rich in China, but at present, only the laboratory where the applicant is located carries out artificial inoculation and identification on the domestic few varieties of the berberis and the Chinese mahonia for the alternate hosts of the wheat stripe rust. Before identification, the germination and seedling raising of the primary host berberis thunbergii and Chinese mahonia seeds are the most important links for determining the identification process.
As for the existing method for germinating the seeds of the berberis thunbergii and the Chinese mahonia, the method relates to chemical agent treatment in the early stage, the germination procedure is complicated, the time consumption is long, the overall germination efficiency is low, certain environmental pollution can be caused, and the method is not beneficial to the development of identification work. The invention provides a simple and effective system for sprouting the seeds of the berberis thunbergii and the Chinese mahonia, ensures the seeds of the berberis thunbergii and the Chinese mahonia to sprout on the basis of simplified treatment, and has the advantages of simple operation, time saving and high efficiency.
Disclosure of Invention
The purpose of the invention is as follows: aiming at the defects of the prior art, the invention provides a simple and effective method for germinating the seeds of the berberis thunbergii and the Chinese mahonia stem into seedlings. The method can not only improve the seed germination rate, but also accelerate the seed germination and seedling raising speed.
The technical scheme is as follows: the invention relates to a method for germinating wheat stripe rust fungus primary host seeds into seedlings, which is characterized by comprising the following steps: the method comprises the following steps:
a method for germinating wheat stripe rust fungus primary host seeds comprises the following steps:
(1) collecting and storing: collecting mature seeds and storing the seeds at low indoor temperature;
(2) seed pretreatment: washing pulp remained on the neutron surface by using clean water, and cleaning the pulp by using clean water after disinfection treatment;
(3) pretreatment of the container: putting W-shaped folded filter paper or gauze at the bottom of the container;
(4) seed transferring: flatly paving the seeds in the step (2) in the container in the step (3), pouring water for seed soaking treatment, and covering a container cover;
(5) germination: placing the container in the step (4) in an incubator for germination at 16-20 ℃, pouring off excessive water in the container at intervals of 24h, and continuing culturing until neutrons germinate;
(6) seedling formation: and (4) transferring the germinated seeds in the step (5) into a culture pot, culturing in a growth chamber, and culturing into seedlings after 3-4 true leaves grow.
Preferably, the sterilization treatment in the step (2) is 0.5% (m/v) potassium permanganate (KMnO)4) Treating the solution for 20-30 min.
Preferably, the container used in step (3) is made of a sterilized material.
Preferably, in the step (3), the bottom of the container is a stack of filter paper or gauze.
Preferably, the seeds in step (4) are spread on the concave area of the W-shaped filter paper or gauze.
Preferably, the seed soaking treatment in the step (4) is to add purified water to the container, wherein the purified water is not higher than the seed.
Preferably, the temperature of the culture box in the step (5) is required to be 16 ℃.
Preferably, the water used in the step is pure water.
Preferably, in the step (6), the bottom of the culture pot is filled with a seedling raising substrate with the relative humidity of 50% -60%
Advantageous effects
Compared with the prior art, the invention has the following remarkable advantages: the method only needs simple treatment, and the seeds of the berberis thunbergii and the Chinese mahonia can germinate and grow into seedlings within about 9 days, and the germination rate reaches up to 100 percent. On the basis of ensuring the germination rate, the method has the characteristics of simplicity, convenience and high efficiency, avoids the problem of environmental pollution caused by the traditional chemical agent treatment, and provides guarantee for accelerating the indoor artificial identification of the rust stripe wheat bacterium primary host.
Drawings
FIG. 1 shows the Berberis seeds that have germinated in the culture dish under the conditions of the present method.
FIG. 2 shows the germinated Mahonia seeds in the petri dish under the conditions of the present method.
FIG. 3 shows the growth of Berberis seedlings in the culture pot.
FIG. 4 shows the growth of seedlings of Mahonia in culture pots.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following further describes the technical solution of the present invention with reference to the drawings and the embodiments.
Example 1: berberis seeds germinate into seedlings
The embodiment of the invention provides a method for germinating wheat stripe rust host-transformed seed into seedlings, which is characterized by comprising the following steps: the method comprises the following steps:
(1) collecting and storing: collecting the mature fresh Berberis seeds in the current year and storing at a low temperature of 4 ℃ in an indoor refrigerator;
(2) seed pretreatment: washing pulp remained on neutron surface with clean water, and placing seed in 0.5% (m/v) potassium permanganate (KMnO)4) Sterilizing in the solution for 20-30min, and washing with purified water for 2-3 times;
(3) pretreatment of the container: 2-3 pieces of sterilized circular filter paper folded into a W shape are placed at the bottom of the disposable culture dish;
(4) seed transferring: flatly paving the seeds in the step (2) in the culture dish in the step (3), pouring purified water which submerges the seeds into the culture dish for seed soaking treatment, and covering the culture dish;
(5) germination: placing the culture dish in the step (4) in an incubator for germination at 16 ℃, pouring excessive water in the container at an interval of 24 hours in the germination process, and continuing culturing until neutrons germinate;
(6) seedling formation: and (3) after a seedling raising substrate with the humidity of 50% -60% is placed at the bottom of the culture pot, the germinated seeds in the step (5) are transferred to the culture pot and are cultured in a growth chamber, and when 3-4 true leaves grow, the seedlings are cultured.
By adopting the method of the embodiment, as shown in figure 1, fresh berberis seeds are successfully germinated; as shown in FIG. 2, the germinated Berberis seeds were cultivated into seedlings. Wherein the germination rate of the Berberis seeds is 100%, the germination time lag is about 9 days, and the germination lasts for 5 days.
Example 2: chinese mahonia seed germination
The embodiment of the invention provides a method for germinating wheat stripe rust host-transformed seed into seedlings, which is characterized by comprising the following steps: the method comprises the following steps:
(1) collecting and storing: collecting the seeds of the fresh Chinese mahonia matured in the current year and storing the seeds in an indoor refrigerator at a low temperature of 4 ℃;
(2) seed pretreatment: washing pulp remained on neutron surface with clean water, and placing seed in 0.5% (m/v) potassium permanganate (KMnO)4) Sterilizing in the solution for 20-30min, and washing with purified water for 2-3 times;
(3) pretreatment of the container: 2-3 pieces of sterilized circular filter paper folded into a W shape are placed at the bottom of the disposable culture dish;
(4) seed transferring: flatly paving the seeds in the step (2) in a concave area of W-shaped filter paper or gauze in the culture dish in the step (3), pouring purified water with the height of the seeds being over for soaking, and covering a culture dish cover;
(5) germination: placing the culture dish in the step (4) in an incubator for germination at 16 ℃, pouring excessive water in the container at an interval of 24 hours in the germination process, and continuing culturing until neutrons germinate;
(6) seedling formation: and (3) after a seedling raising substrate with the humidity of 50% -60% is placed at the bottom of the culture pot, the germinated seeds in the step (5) are transferred to the culture pot and are cultured in a growth chamber, and when 3-4 true leaves grow, seedlings are cultured.
With the method of this example, as shown in FIG. 3, fresh Mahonia seeds were successfully germinated; as shown in FIG. 4, the germinated Mahonia seeds were grown into seedlings. The germination rate of the Chinese mahonia seeds is 95%, the germination time lag is about 9 days, and the germination lasts for 5 days.
According to the invention, the filter paper is folded into a W-shaped structure, so that the seed germination speed is increased, and the germination rate is very high and can reach 100%.
Claims (9)
1. A method for germinating wheat stripe rust fungus primary host seeds is characterized in that: the method comprises the following steps:
(1) collecting and storing: collecting mature seeds and storing the seeds at low indoor temperature;
(2) seed pretreatment: washing pulp remained on the neutron surface by using clean water, and cleaning the pulp by using clean water after disinfection treatment;
(3) pretreatment of the container: putting W-shaped folded filter paper or gauze at the bottom of the container;
(4) seed transferring: flatly paving the seeds in the step (2) in the container in the step (3), pouring water for seed soaking treatment, and covering a container cover;
(5) germination: placing the container in the step (4) in an incubator for germination at 16-20 ℃, pouring off excessive water in the container at intervals of 24h, and continuing culturing until neutrons germinate;
(6) seedling formation: and (4) transferring the germinated seeds in the step (5) into a culture pot, culturing in a growth chamber, and culturing into seedlings after 3-4 true leaves grow.
2. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: the sterilization treatment in the step (2) is 0.5 percent (m/v) of potassium permanganate (KMnO)4) Treating the solution for 20-30 min.
3. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: the container used in the step (3) adopts a sterilized material.
4. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: and (4) stacking filter paper or gauze at the bottom of the container in the step (3).
5. The method for germination of the stripe rust wheat primary host seed into seedlings according to claim 1, which comprises the following steps: and (4) flatly paving the seeds in the concave area of the W-shaped filter paper or gauze.
6. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: the seed soaking treatment in the step (4) is to add purified water which is over the height of the seeds into the container.
7. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: the temperature of the incubator in the step (5) is required to be 16 ℃.
8. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: the water used in the step is purified water.
9. The method for germination of puccinia striiformis f.sp.tritici into seedlings as claimed in claim 1, wherein: and (4) in the step (6), a seedling culture substrate with the relative humidity of 50% -60% is arranged at the bottom in the culture pot.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117384943A (en) * | 2023-12-07 | 2024-01-12 | 西北农林科技大学深圳研究院 | Wheat pyruvate kinase TaPKc and application thereof |
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2020
- 2020-03-18 CN CN202010192776.3A patent/CN111373898A/en active Pending
Patent Citations (4)
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| CN101455158A (en) * | 2008-01-24 | 2009-06-17 | 孙超 | Sexual multiplication method of Mahonia duclouxiana Gagnep.seeds |
| CN205681809U (en) * | 2016-06-16 | 2016-11-16 | 中国农业科学院油料作物研究所 | A kind of high flux seed germination box of band cistern |
| CN106577151A (en) * | 2017-01-03 | 2017-04-26 | 黄振忠 | Chinese mahonia nutrition cup seedling-raising method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117384943A (en) * | 2023-12-07 | 2024-01-12 | 西北农林科技大学深圳研究院 | Wheat pyruvate kinase TaPKc and application thereof |
| CN117384943B (en) * | 2023-12-07 | 2024-02-27 | 西北农林科技大学深圳研究院 | Wheat pyruvate kinase TaPKc and application thereof |
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Application publication date: 20200707 |