CN111366624A - Triglyceride test paper with temperature label, preparation process and use method - Google Patents
Triglyceride test paper with temperature label, preparation process and use method Download PDFInfo
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- CN111366624A CN111366624A CN202010252208.8A CN202010252208A CN111366624A CN 111366624 A CN111366624 A CN 111366624A CN 202010252208 A CN202010252208 A CN 202010252208A CN 111366624 A CN111366624 A CN 111366624A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3274—Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
Abstract
The invention provides triglyceride test paper with a temperature label, a preparation process and a use method, and belongs to the technical field of electrochemistry. The electrochemical triglyceride test paper with the temperature label is formed by sequentially attaching a triglyceride test paper substrate layer with the temperature label, an electrochemical metal electrode layer, a covering layer and an upper interlayer from bottom to top, wherein the electrochemical electrode layer consists of an electrochemical working electrode, an electrochemical counter electrode, a full blood trigger electrode, a switch-on electrode and a metal electrode of a temperature sensor, and the electrochemical working electrode, the electrochemical counter electrode, the full blood trigger electrode, the switch-on electrode and the temperature sensor are sequentially designed according to the sequence from near to far of the top end distance of a blood sucking end. The invention realizes an electrochemical triglyceride test paper technology of an integrated temperature sensor for the first time, so that different types of test paper achieve the effect of automatic temperature compensation by directly measuring the temperature near a reaction area.
Description
Technical Field
The invention belongs to the technical field of electrochemistry, and particularly relates to triglyceride test paper with a temperature label and a using method thereof.
Background
The lipoprotein lipase and triglyceride in blood are subjected to electrochemical reaction, charge transfer generated in the reaction process forms current, the detected current is analyzed by a triglyceride tester, the temperature is measured by using a temperature sensor in the triglyceride tester, and the triglyceride concentration in the blood can be measured by combining a temperature compensation curve and a standard calibration concentration curve library. However, if the mobile medical digital terminal is used for a medical mobile phone or a medical pad, the medical terminal itself will generate heat, and the internal temperature sensor cannot measure the temperature of the test paper reaction area or the reaction environment, and in such a scenario, an independent temperature compensation electronic card needs to be inserted into the medical terminal in advance to tell the machine to measure the temperature of the environment, which greatly increases the measurement cost.
In the prior art, the current values are tested by using quality control liquids with the same triglyceride concentration under the conditions of different environmental temperatures, and the current values tested under the same triglyceride concentration are found to have large differences, and the obtained experimental data are as follows:
TABLE 1 data of triglyceride concentration of 3.12mmol/L at 10 deg.C, 14 deg.C, 17 deg.C, 23 deg.C, and 32 deg.C
From the above experimental data, it can be seen that the temperature has a great influence on the current value, and the temperature to be tested is the ambient temperature, and is not the temperature of the reaction region, and the instrument itself generates heat, which causes the temperature of the reaction region and the ambient temperature not to be the same, and causes a great deviation in the final test result even after temperature compensation.
Meanwhile, in such a scenario, a separate temperature compensation electronic card needs to be inserted into the medical terminal in advance to tell the machine to measure the ambient temperature, which greatly increases the measurement cost.
However, the electrode for measuring temperature in the prior art has very high requirements on materials, and the temperature electrode and the temperature sensor are not manufactured into a relevant measuring instrument by adopting a printing process.
In summary, the prior art has a problem that, when a medical terminal is used as a triglyceride monitoring terminal, a common triglyceride test paper cannot accurately measure the triglyceride concentration of a blood sample, and the temperature sensor inside the medical terminal cannot accurately measure the temperature due to the self-heating problem of the medical terminal.
Disclosure of Invention
In order to overcome the problems in the background art, the invention provides the triglyceride test paper with the temperature label, the preparation process and the use method thereof, the aim of automatic temperature compensation is realized by measuring the temperature of the reaction area of the test paper through a sensor, and the measurement result is more accurate.
The invention adopts the following technical scheme:
the preparation process of the triglyceride test paper with the temperature label is characterized by comprising the following steps of:
Plating a 20-50nm gold layer on the PET by using magnetron sputtering equipment, and controlling the sputtering pressure to be 0.5 Pa;
step 2, the prepared medicine is dripped into the corresponding area
After evaporation is finished, a layer of SU-8 photoresist is coated in a rotating mode, a mask is used for photoetching SU-8, the irradiated part can be kept and is not washed away by SU-8 boiling liquid, and a layout of a temperature measuring electrode is kept;
step 3, after baking, attaching a covering layer, adding lipoprotein lipase in a reflecting area, attaching the covering layer and an interlayer;
lipoprotein lipase reacts with triglycerides in the blood as follows:
H2O2+Fe2+——→H2O+Fe3+。
the electrochemical triglyceride test paper with the temperature label is formed by sequentially attaching a triglyceride test paper substrate layer with the temperature label, an electrochemical metal electrode layer, a covering layer and an upper interlayer from bottom to top, wherein the electrochemical electrode layer consists of an electrochemical working electrode, an electrochemical counter electrode, a starting-up connection electrode, a full blood trigger electrode, a temperature measuring electrode and a metal electrode of a temperature sensor. The cover layer is composed of a septum and a reaction region, and the lipoprotein lipase is disposed in the reaction region of the cover layer.
According to the distance from the top end of the blood sucking end, an electrochemical working electrode, an electrochemical counter electrode, a temperature sensor, a full blood trigger electrode, a starting connection electrode and a temperature measurement electrode are sequentially arranged from near to far, the electrochemical working electrode is independently arranged on the left side of the starting connection electrode, the electrochemical working electrode and the electrochemical counter electrode are used for measuring characteristic current, the starting connection electrode is connected with the full blood trigger electrode, the starting connection electrode and the full blood trigger electrode are positioned between the electrochemical working electrode and the electrochemical counter electrode, the starting connection electrode is positioned on the left side of the full blood trigger electrode, the right side of the electrochemical counter electrode is provided with the temperature measurement electrode, the electrochemical counter electrode is connected with the temperature measurement electrode through the temperature sensor, and the temperature measurement electrode is used for measuring the resistance value of the temperature sensor, so that the temperature of a reaction area is.
The further technical scheme is that an electrochemical working electrode, an electrochemical counter electrode, a full blood trigger electrode, a starting switch-on electrode and a temperature sensor on the electrochemical electrode layer are all metal electrodes.
The further technical scheme is that the triglyceride test paper substrate layer with the temperature label is made of any material of polyethylene terephthalate, polyvinyl chloride and polypropylene.
The further technical proposal is that the upper end of the upper cover plate of the upper interlayer is provided with air holes.
The use method of the triglyceride test paper with the temperature label comprises the following steps:
the test paper carries out electrochemical reaction with blood through lipoprotein lipase fixed on an electrochemical working electrode, characteristic current is collected by the electrode, and therefore the concentration of triglyceride is measured. The temperature measured by the temperature sensor can be determined by measuring the resistance of the temperature sensor, the temperature estimation of the reaction area is determined, a preset temperature compensation curve is called from the corresponding medical mobile phone, so that the temperature compensation is carried out on the measured electrochemical current, and the concentration of triglyceride in blood is analyzed and measured by using a measured concentration standard curve library.
The invention has the beneficial effects that:
the invention realizes an electrochemical triglyceride test paper with a temperature label suitable for a medical mobile phone or a medical pad for the first time, and the temperature sensor is additionally arranged between the electrochemical electrode pair and the rightmost electrode, so that the triglyceride test paper has the capability of measuring the temperature near a reaction area, and the temperature measured by the triglyceride test paper is fed back to a medical terminal, thereby carrying out accurate temperature compensation and ensuring that the triglyceride measurement result is accurate.
Drawings
FIG. 1 is a schematic structural diagram of an electrochemical triglyceride test strip with a temperature tag according to an embodiment of the present invention;
FIG. 2 is a schematic view of a substrate layer of a triglyceride test strip with a temperature tag;
FIG. 3 is a schematic view of an electrochemical metal electrode layer;
FIG. 4 is a schematic view of a cover layer;
FIG. 5 is a schematic representation of a lipoprotein lipase layer;
FIG. 6 is a schematic view of an upper spacer layer;
FIG. 7 is a flow chart of the printed temperature electrode fabrication process of the present invention.
In the context of figure 1 of the drawings,
1. a triglyceride test paper substrate layer with a temperature label;
2. an electrochemical metal electrode layer;
2-1, an electrochemical working electrode;
2-2, an electrochemical counter electrode;
2-3, starting up to switch on the electrode;
2-4, triggering electrodes when full blood exists;
2-5, temperature measuring electrodes;
2-6, a temperature sensor;
3. a cover layer is arranged on the outer surface of the substrate,
3-1, a septum layer;
3-2, a reaction zone;
4. lipoprotein lipase;
5. an upper interlayer;
5-1, an upper cover plate;
5-2 and pores.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described below clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, the temperature-labeled triglyceride test strip provided by the embodiment of the present invention includes: the test paper comprises a triglyceride test paper substrate layer 1 with a temperature label, an electrochemical metal electrode layer 2, a covering layer 3 and an upper interlayer 5.
As shown in fig. 2 and 4, the triglyceride test paper with the temperature label is formed by sequentially attaching a substrate layer 1 with the temperature label test paper, an electrochemical metal electrode layer 2, a middle spacer layer 3, a lipoprotein lipase layer 4 and an upper spacer layer 5 from bottom to top. The cover layer 3 is composed of a septum and a reaction region 3-2, and a lipoprotein lipase 4 is disposed in the reaction region 3-2 of the cover layer 3.
As shown in FIG. 3, the electrochemical metal electrode layer 2 comprises an electrochemical working electrode 2-1, an electrochemical counter electrode 2-2, a power-on electrode 2-3, a full blood trigger electrode 2-4, a temperature measuring electrode 2-5 and a temperature sensor 2-6, and the electrochemical metal electrode layer 2 is a metal electrode.
Preferably, the triglyceride test paper substrate layer 1 with the temperature label is made of any material of polyethylene terephthalate, polyvinyl chloride and polypropylene.
Preferably, the electrochemical metal electrode layer 2 consists of an electrochemical working electrode 2-1, an electrochemical counter electrode 2-2, a power-on switch-on electrode 2-3, a full blood trigger electrode 2-4, a temperature measuring electrode 2-5 and a temperature sensor 2-6, and all are metal electrodes.
As shown in fig. 5 and 6, the electrochemical working electrode 2-1, the electrochemical counter electrode 2-2, the temperature sensor 2-6, the full blood trigger electrode 2-4, the power-on switch-on electrode 2-3 and the temperature measuring electrode 2-5 are arranged in sequence from near to far according to the distance from the top end of the blood sucking end, and the temperature sensor 2-6 is a thermosensitive metal wire.
The electrochemical working electrode 2-1 and the electrochemical counter electrode 2-2 are used for measuring characteristic current and are independently arranged on the left side of the start-up connection electrode 2-3, the start-up connection electrode 2-3 is connected with the full blood trigger electrode 2-4, the start-up connection electrode 2-3 can act when the test paper is inserted in the start-up device, and the indicator for indicating blood drop measurement is prompted on the instrument; the 2-4 full blood trigger electrode has the function of prompting that the blood adding amount is insufficient, and if the test is accurate, the dropped blood amount is enough, the insufficient full blood trigger electrode 2-4 can be triggered to prompt that the blood adding amount is insufficient, and the starting-up switch-on electrode 2-3 and the full blood trigger electrode 2-4 are positioned between the electrochemical working electrode 2-1 and the electrochemical counter electrode 2-2, and the starting-up switch-on electrode 2-3 is positioned at the left side of the full blood trigger electrode 2-4, the right side of the electrochemical counter electrode 2-2 is provided with a temperature measuring electrode 2-5, the electrochemical counter electrode 2-2 is connected with the temperature measuring electrode 2-5 through the temperature sensor 2-6, and the temperature measuring electrode 2-5 is used for measuring the resistance value of the temperature sensor 2-6, so that the temperature of the reaction area 3-2 is obtained.
Preferably, the upper isolation layer 5 consists of an upper cover plate 5-1 and air holes 5-2, and the upper end of the upper cover plate 5-1 is provided with the air holes 5-2.
As shown in fig. 7, the preparation process of the triglyceride test paper with the temperature label of the invention comprises the following steps:
Plating a 20-50nm gold layer on the PET by using magnetron sputtering equipment, and controlling the sputtering pressure to be 0.5 Pa;
step 2, the prepared medicine is dripped into the corresponding area
After evaporation is finished, a layer of SU-8 photoresist is coated in a rotating mode, a mask is used for photoetching SU-8, the irradiated part can be kept and is not washed away by SU-8 boiling liquid, and a layout of a temperature measuring electrode is kept;
and 3, attaching a middle partition hydrophilic membrane (a covering layer and an upper partition layer) after baking, and adding lipoprotein lipase into the reflecting area.
And 4, cutting into small strips and packaging into a barrel.
The principle of this reaction is as follows:
H2O2+Fe2+——→H2O+Fe3+
the application method of the triglyceride test paper with the temperature label comprises the following steps:
firstly, a blood sucking port of the test paper is close to fresh fingertip blood, the blood is sucked into the reaction area 3-2 by the test paper and is filled in the reaction area 3-2, and the test paper is subjected to electrochemical reaction with the blood through lipoprotein lipase fixed on an electrochemical working electrode 2-1.
And secondly, measuring the triglyceride concentration by using a triglyceride detector (medical terminal detector) through the characteristic current collected by the electrochemical working electrode 2-1 and utilizing a standard corresponding curve function of the concentration and the current.
And thirdly, measuring the temperature of the reaction area 3-2 for temperature compensation calibration due to the electrochemical enzyme method. The temperature of the reaction zone 3-2 is measured by a temperature sensor 2-6 located closer to the zone.
The measured temperature of the temperature sensor 2-6 can be determined by measuring the resistance of the temperature sensor 2-6, so that the temperature estimation of the reaction area 3-2 is determined, a preset temperature compensation curve is called from a corresponding medical mobile phone, so that the temperature compensation is performed on the measured electrochemical current, and the concentration of the electrochemical triglyceride in the blood is analyzed and measured by using a measured concentration standard curve library.
The temperature measuring electrodes 2-5 and the temperature sensors 2-6 start to work to measure the temperature of the reaction area after the blood is dropped and the test is started, and the temperature compensation operation is automatically carried out according to the measured temperature. The function of the air hole 5-2 is to discharge air through the air hole 5-2 when the blood enters the reaction region 3-2 and is filled.
The application of the principles of the present invention will be further described with reference to the accompanying drawings in which:
temperature labeled triglyceride test paper substrate layer 1: is made of any one of polyethylene terephthalate (PET), polyvinyl chloride (PVC) and polypropylene (PP).
Electrochemical electrode layer 2: for example, the electrochemical working electrode 2-1, the electrochemical counter electrode 2-2, the power-on electrode 2-3 and the full blood trigger electrode 2-4 can all adopt noble metals. The temperature sensors 2-6 are made of temperature-sensitive resistance materials. The temperature sensors 2-6 are manufactured by adopting a magnetron sputtering coating process of temperature-sensitive resistance materials. Preferably, the temperature-sensitive resistance material of the temperature sensor is platinum, copper, iron, nickel, etc.
The electrochemical working electrode 2-1 and the electrochemical counter electrode 2-2 measure triglyceride in blood in the reaction area 3-2, temperature compensation correction is carried out through temperature results measured by the temperature sensor 2-6, and finally triglyceride concentration measurement values are displayed on corresponding medical mobile phones or medical pads and other medical terminals.
The full blood trigger electrode 2-4 is positioned at the lowest end of the reaction area 3-2, when blood enters from the blood sucking port, the full blood trigger electrode 2-4 test paper at the bottom of the reaction area 3-2 is required to send a measurement countdown to a corresponding medical terminal, the medical terminal starts a countdown test, and the design of the full blood trigger electrode 2-4 can avoid the inaccuracy of a measurement value caused by the fact that the whole reaction area is not filled with the blood; one of the electrodes 2-3 is switched on when the test paper is inserted into the corresponding medical terminal, the electrodes 2-3 are connected with two ends of an interruption circuit in the medical terminal, so that the test paper circuit and the circuit of the medical terminal form a complete loop, the medical terminal is automatically switched on, the test paper is drawn out after measurement is completed, and the medical terminal is automatically switched off.
The impedance of the temperature sensors 2-6 in the reflecting area is determined by the temperature, and different impedances are corresponding to different reaction temperatures; lipoprotein lipase 4 provides the enzyme for the electrochemical reaction to take place.
An upper cover 5-1 for covering the reaction area 3-2, the front end of the upper cover 5-1 having an air hole 5-2 for discharging air when blood enters the reaction area 3-2.
The upper cover plate 5 is positioned at the upper end of the middle partition layer 3-1 and is used for protecting the reaction area 3-2 from being interfered by the external environment.
Practical embodiments of the invention
Experimental equipment: triglyceride detector module, quality control liquid with different concentration gradients, and liquid transfer gun
Meanwhile, a part of test paper is randomly extracted from the traditional triglyceride test paper and the triglyceride test paper of the invention respectively, 5 triglycerides with gradient are selected as the concentration of the quality control liquid, 5-10 test paper are tested for each concentration, the temperature of the tested external environment is 23 ℃, the average value of the concentration values of the measured triglycerides is taken as the measured value of the concentration of the quality control, and the test results of the two are compared, wherein the test results are shown in the following table:
TABLE 1 results of the test on the triglyceride test paper of the present invention (unit: mmol/L)
TABLE 2 test results of conventional triglyceride test paper (unit: mmol/L)
Finally, the comparison of the test results of the two shows that the test result of the automatic temperature compensation of the temperature of the triglyceride test reaction area is more accurate than that of the traditional triglyceride test paper for detecting the environmental temperature, and the deviation of the test result with the biochemical value of LDL is small.
The temperature has a great influence on the test result, the traditional test paper is the test environment temperature and is not the temperature of the reaction area, if the temperature of the instrument is counted again, the temperature of the reaction area is different from the environment temperature, the final test result has larger deviation even through temperature compensation, and the measurement mode needs an independent temperature compensation electronic card to be inserted into a medical terminal in advance to tell the machine to measure the temperature of the environment, so the measurement cost is greatly increased.
The test paper of the invention adds a temperature sensor between the electrochemical electrode pair and the rightmost electrode, so that the triglyceride test paper has the capability of measuring the temperature near the reaction area, and the temperature measured by the triglyceride test paper is fed back to the medical terminal, thereby carrying out accurate temperature compensation and ensuring that the triglyceride measurement result is more accurate.
The temperature sensor is used for measuring the temperature of the test paper chemical reaction area, but not the ambient temperature, because the original medical terminal is used for measuring the ambient temperature, the difference between the temperature of the reaction area and the ambient temperature is large due to the fact that the instrument generates heat and the heat released by chemical reaction possibly causes inaccurate temperature compensation, the temperature of the reaction area is measured in real time, the temperature compensation is accurate, the measurement result is accurate, the temperature sensor is required to be located at the position, and the temperature of the reaction area cannot be measured at other places.
Because the traditional unable automatic measurement reaction zone temperature or ambient temperature to need to warm independently mend the electronic card and insert and can only tell the instrument current ambient temperature at the terminal, the shortcoming is exactly that the measurement is troublesome, in addition can't test reaction zone temperature and can have the condition of measuring not too accurately, and this problem has just been solved to present test paper, can the automatic measurement reaction zone temperature, and the testing process is simple and convenient, and the result is also more accurate.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. The preparation process of the triglyceride test paper with the temperature label is characterized by comprising the following steps of:
step 1, printing an electrochemical metal electrode on a triglyceride test paper substrate layer with a temperature label
Plating a 20-50nm gold layer on the PET by using magnetron sputtering equipment, and controlling the sputtering pressure to be 0.5 Pa;
step 2, the prepared medicine is dripped into the corresponding area
After evaporation is finished, a layer of SU-8 photoresist is coated in a rotating mode, a mask is used for photoetching SU-8, the irradiated part can be kept and is not washed away by SU-8 boiling liquid, and a layout of a temperature measuring electrode is kept;
step 3, after baking, attaching a covering layer, adding lipoprotein lipase in a reflecting area, attaching the covering layer and an interlayer;
step 4, cutting into small strips and packaging into a cylinder;
lipoprotein lipase reacts with triglycerides in the blood as follows:
H2O2+Fe2+→H2O+Fe3+。
2. the triglyceride test paper with the temperature label is characterized by being prepared by adopting a preparation process of electrochemical triglyceride test paper with the temperature label, wherein the electrochemical triglyceride test paper with the temperature label is formed by sequentially attaching a triglyceride test paper substrate layer with the temperature label, an electrochemical metal electrode layer, a covering layer and an upper interlayer from bottom to top, and the electrochemical electrode layer consists of an electrochemical working electrode, an electrochemical counter electrode, a startup connection electrode, a full blood trigger electrode, a temperature measurement electrode and a metal electrode of a temperature sensor; the covering layer consists of a septum and a reaction area, and the lipoprotein lipase is arranged in the reaction area of the covering layer;
according to the distance from the top end of the blood sucking end, an electrochemical working electrode, an electrochemical counter electrode, a temperature sensor, a full blood trigger electrode, a starting connection electrode and a temperature measuring electrode are sequentially arranged from near to far, the electrochemical working electrode is independently arranged on the left side of the starting connection electrode, the electrochemical working electrode and the electrochemical counter electrode are used for measuring characteristic current, the starting connection electrode is connected with the full blood trigger electrode, the starting connection electrode and the full blood trigger electrode are located between the electrochemical working electrode and the electrochemical counter electrode, the starting connection electrode is located on the left side of the full blood trigger electrode, the right side of the electrochemical counter electrode is provided with the temperature measuring electrode, the electrochemical counter electrode is connected with the temperature measuring electrode through the temperature sensor, and the temperature measuring electrode is used for measuring the resistance value of the temperature sensor.
3. The triglyceride test paper with the temperature label of claim 2, wherein the electrochemical working electrode, the electrochemical counter electrode, the full blood trigger electrode, the power-on electrode and the temperature sensor on the electrochemical electrode layer are all metal electrodes.
4. The temperature-labeled triglyceride test paper according to claim 2, wherein the temperature-labeled triglyceride test paper substrate layer is made of any one of polyethylene terephthalate, polyvinyl chloride and polypropylene.
5. The triglyceride test strip with the temperature label of claim 2, wherein the upper end of the upper cover sheet of the upper partition layer is provided with air holes.
6. The method for using the triglyceride test paper with the temperature label is characterized in that,
step 1, enabling a blood sucking port of the test paper to be close to fresh fingertip blood, sucking the blood into a reaction area by the test paper and filling the reaction area with the blood, carrying out electrochemical reaction on the test paper and the blood through lipoprotein lipase fixed on an electrochemical working electrode, and forming current through charge transfer generated in the reaction process;
when blood enters the blood sucking end from the top and reaches the full blood triggering electrode at the bottom, the test paper sends out a measurement countdown to the corresponding medical terminal;
step 2, a triglyceride detector is used for collecting characteristic current through a counter electrode, and the concentration of triglyceride is measured by combining a standard concentration-current calibration curve;
when the test paper is inserted into the corresponding medical terminal, the test paper is started up to be connected with two ends of an interruption circuit in the medical terminal, so that the test paper circuit and the circuit of the medical terminal form a complete loop;
step 3, measuring the temperature of the reaction area by using a temperature sensor, measuring the reaction current and simultaneously detecting the resistance of the temperature sensor, determining the temperature measured by the temperature sensor through the resistance of the temperature sensor, and determining the temperature estimation of the area;
the impedance of the temperature sensor in the reaction area is determined by the temperature, and the impedance corresponds to different impedances according to different reaction temperatures;
step 4, calling a preset temperature compensation curve from the corresponding medical terminal so as to perform temperature compensation on the measured electrochemical current;
and 5, analyzing and measuring the concentration of the triglyceride in the blood by using the standard curve library of the measured concentration.
7. The method of using a triglyceride test strip with a temperature marker according to claim 6, wherein in step 1, the medical terminal starts a countdown test, and the full blood trigger electrode is designed to avoid inaccurate measurement values caused by the fact that the whole reaction area is not filled with blood.
8. The method for using the triglyceride test paper with the temperature label in the claim 6, wherein in the step 2, the medical terminal is automatically powered on, and the medical terminal is automatically powered off by drawing out the test paper after the measurement is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010252208.8A CN111366624A (en) | 2020-04-01 | 2020-04-01 | Triglyceride test paper with temperature label, preparation process and use method |
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| CN202010252208.8A CN111366624A (en) | 2020-04-01 | 2020-04-01 | Triglyceride test paper with temperature label, preparation process and use method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116606837A (en) * | 2023-07-17 | 2023-08-18 | 南京晶捷生物科技有限公司 | Complex enzyme liquid for electrochemical detection of triglyceride, detection test paper and sensor |
| WO2023186389A1 (en) * | 2022-03-28 | 2023-10-05 | Senslab Gesellschaft Zur Entwicklung Und Herstellung Bioelektrochemischer Sensoren Mbh | Method and sensor for determining a plasma-related analyte concentration in whole blood |
-
2020
- 2020-04-01 CN CN202010252208.8A patent/CN111366624A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023186389A1 (en) * | 2022-03-28 | 2023-10-05 | Senslab Gesellschaft Zur Entwicklung Und Herstellung Bioelektrochemischer Sensoren Mbh | Method and sensor for determining a plasma-related analyte concentration in whole blood |
| CN116606837A (en) * | 2023-07-17 | 2023-08-18 | 南京晶捷生物科技有限公司 | Complex enzyme liquid for electrochemical detection of triglyceride, detection test paper and sensor |
| CN116606837B (en) * | 2023-07-17 | 2023-10-20 | 南京晶捷生物科技有限公司 | Complex enzyme liquid for electrochemical detection of triglyceride, detection test paper and sensor |
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