CN111366409A

CN111366409A - Sampling smear method of brush and application thereof

Info

Publication number: CN111366409A
Application number: CN201911117266.3A
Authority: CN
Inventors: 浦洪杰
Original assignee: Pu Zhenxuan; Pu Zhenyue
Current assignee: Pu Zhenxuan; Pu Zhenyue
Priority date: 2019-11-15
Filing date: 2019-11-15
Publication date: 2020-07-03
Also published as: CN111929107A

Abstract

A sampling smear method of a brush and application thereof, in particular to a sampling smear method of a clinical test sputum specimen of a brush and application thereof, which are used for solving the problems of poor effect, troublesome use, low smear quality and the like of the sputum specimen using a bamboo stick with a stubble cutting function. The pen brush with the puncture sampling smear performance is adopted to perform the puncture sampling smear on the sputum specimen, so that the solid matters in the specimen can be effectively separated, the sampling quality is improved, and the effect of improving the detection rate of pathogens is also achieved when the pen brush is used for the sputum specimen of the tuberculosis patient.

Description

Sampling smear method of brush and application thereof

Technical Field

A sampling smear method for body fluid inspection of a brush and application thereof, in particular to a sampling smear method for clinical inspection of sputum specimens of a brush and application thereof.

Background

The sputum specimen testing method mainly comprises the following steps of preparing a hard sputum specimen by using a bamboo stick, a cotton swab, an inoculating loop, a brush, a dropper, a pipette and the like, wherein the hard sputum specimen testing method mainly comprises the steps of preparing a hard sputum specimen by using a bamboo stick, a soft sputum specimen, a hard sputum specimen and the like, and the hard sputum specimen is prepared by using a brush, the hard sputum specimen is prepared by using the conventional brush, the method is not applicable to the method is a traditional technical scheme that the hard sputum specimen is prepared by using the hard sputum specimen, the hard sputum specimen is prepared by using the conventional method, the conventional method is the conventional method, the method is not applicable to the method is the method.

Disclosure of Invention

In order to solve the problems of poor sampling effect, troublesome use, low smear quality and the like of the conventional sputum specimen sampling smear using a bamboo stick for cutting off the stubble, a sampling smear method of a clinical examination sputum specimen by a brush and application thereof are provided.

The invention solves the technical problem and provides a sampling smear method of a clinical sputum specimen by a brush, which is characterized by a puncture sampling smear method.

The invention solves the technical problem and provides a sampling smear method of a brush for clinically examining a sputum specimen of a tuberculosis patient, which is characterized by a puncture sampling smear method.

The invention solves the technical problem and provides the application of a sampling smear of a clinical examination sputum specimen of a brush, which is characterized by being used for the sampling smear of the sputum specimen of a tuberculosis patient.

① brush bristles penetrate through a sputum specimen for a single time or multiple times, and are used for embedding solid matters into gaps of the bristles, fixing the solid matters and simultaneously squeezing saliva and other low-viscosity liquids adsorbed by the bristles, ② brush bristles impact the bottom of a container and are used for deforming the bristles to twist and tear the solid matters to primarily separate the solid matters, ③ brush withdrawing is used for pulling back the brush and tearing, clamping and adhering the solid matters to complete separation and sampling, and redundant specimens can be rubbed on the wall of the container after sampling.

Because different from ordinary pathogen, the number of tubercle bacillus in the sputum specimen is often few and mostly distributed in the solid matter of sputum specimen, tuberculosis patient's sputum specimen only through the puncture sample smear method, just can effectively separate the solid matter in the sputum specimen, gets better pathogen relevance ratio, and if dip in with the brush hair and adsorb the sample then can only adsorb liquid specimens such as saliva, can not effectively separate the sample to the solid matter in the sample, can lead to the relevance ratio greatly reduced of pathogen, is wrong sample mode. For the above reasons: on one hand, the pen-brush puncture sampling smear method for clinical examination of tuberculosis patient sputum specimens has the advantages that the convenient and effective separation sampling is realized, and the effect of improving the pathogen detection rate can be achieved; on the other hand, the sampling smear use of the clinical examination tuberculosis patient sputum specimen by the pen-brush is necessarily the sampling smear use for adopting the puncture sampling smear method because no other sampling smear method can be used for the use except the puncture sampling smear method and the erroneous dip-adsorption sampling smear method.

The puncturing sampling smear performance of the brush is that ① brush bristles can puncture or pierce a sputum specimen when sampling, and rebound after withdrawing or colliding the bottom of a container and withdrawing and the like cancel external force, so that the effects of twisting, adhering, wrapping, tearing and the like of solid matters in the sputum specimen can be realized, and the capability of separating and sampling is completed, ② brush bristles can coat and transfer the specimen to various carriers such as a slide, a culture medium, a test tube inner wall and the like, and the performance of the brush is generally possessed.

The brush mainly comprises a rod body 1 and brush bristles 2 at the end part, the connecting mode of the rod body and the brush bristles can be various modes such as implantation, bonding, fusion and the like, the length of the brush bristles does not include the length of the part implanted into the rod body when the length of the rod body is extruded, and the brush bristles can be as follows: plastics such as PP, PA, PBT, PET, etc.; metals and alloys such as steel wire, stainless steel wire; non-metals such as glass fibers, carbon fibers, and the like; composite materials such as resin impregnated fiberglass and the like. The length of the bristles may be 3 to 20mm and the diameter may be 0.05 to 0.4mm, the diameter may be 0.05 to 0.13mm when the bristles are made of a metal material, and the diameter may be 0.13 to 0.4mm when the bristles are made of a plastic material. Thus, the pen brush with the puncture sampling smear performance can be prepared.

The brush puncture sampling smear method is used for sputum specimens with higher viscosity, brush hair screened by a brush is compared with burrs of bamboo stick stubble openings, the consistency of the thickness, the length and the strength of the brush hair is good, and the brush hair can be made into the best performance state, compared with the existing sampling method for picking specimens by bamboo stick stubble breaking: has the advantages of good separation and sampling effect on solid matters and convenient use; when the method is used for detecting the tubercle bacillus in the sputum specimen, the nonuniformly distributed tubercle bacillus in the sputum specimen is mostly distributed in the solid matters of the sputum specimen, and the method can better separate the solid matters in the sputum specimen, so that the sputum specimen of a tuberculosis patient can be detected with higher detection rate. The brush puncture sampling smear method overcomes the prejudice that the brush hair can only adhere or dip to absorb the sample, and avoids the defects that the sputum sample detection only utilizes the dipping absorption of the brush hair to only absorb high-fluidity liquid and can not effectively separate and sample solid matters in the sample. In addition, compared with the bamboo stick for breaking stubble, the brush has the following characteristics: the quality of the products is completely consistent, the complex process of stubble breaking treatment is avoided, the coating effect is good, the specimen is not mildewed and polluted, and the like.

Drawings

Reference numeral 1 is a rod body, 2 is bristles. Fig. 1 is a schematic structural diagram of a brush.

Detailed Description

In the first embodiment, the end of a plastic rod body 1 with an implanted pore is implanted with bristles 2 by a numerical control bristle implanting machine, the bristles 2 are 0.13mm PBT brush wires or 0.05mm stainless steel wires, and the bristles 2 are trimmed into a brush with the length of 3mm by a fly-milling machine or a bristle grinding machine to form a brush. The brush is used for sampling and smearing sputum specimens of tuberculosis patients by the following sampling and smearing method, wherein solid matters in the specimens are penetrated by bristles of the brush once or for multiple times, and then the sampled solid matters are coated on carriers such as glass slides, culture media, test tubes and the like.

In the second embodiment, the brush hair 2 is implanted into the end part of the rod body 1 with the implanted hole by using a numerical control hair implanting machine, the brush hair 2 is a PP brush wire with the thickness of 0.18mm, the brush hair 2 is repaired into a brush with the length of 10mm by using a hair flying machine, the step of sampling and smearing the sputum specimen by using the brush is that ① brush hair penetrates the sputum specimen, ② brush hair impacts the bottom of a container, ③ withdraws the brush, the steps from ① to ③ are repeated at multiple points to finish sampling, the sample is coated on a glass slide to finish smearing after redundant specimens are skimmed from the container wall, and the smear is subjected to drying and fixing, acid-resistant dyeing or fluorescent dyeing and the like and then is subjected to microscopic examination or other detection.

In the third embodiment, the brush hair 2 is implanted into the end part of the rod body 1 with the implanted hole at the end part by using a numerical control hair planting machine, the brush hair 2 is PA brush wire with the thickness of 0.4mm or brass wire with the thickness of 0.13mm, the brush hair 2 is trimmed into a length of 20mm by using a hair flying machine or a hair grinding machine to be made into a brush, the step of sampling and smearing the sputum specimen by using the brush hair of ① brush to pierce the sputum specimen, the step of withdrawing the brush ②, the steps of repeating the steps of ① to ② for multiple points to finish sampling, and after sampling, the excessive specimen is removed from the container wall, the sample is coated on specimen glass slides, culture media, test tubes and other carrying objects to finish smearing.

Claims

1. A sampling smear method for clinical test of sputum specimen by pen brush features that a sampling smear method is used for puncture.

2. The method of claim 1, wherein the sputum specimen is a sputum specimen from a tuberculosis patient.

3. The method of claim 1 or 2 wherein the piercing of the sampling smear is a single or multiple sampling process comprising the steps of ① penetration of the brush bristles into the sputum specimen, ② impact of the brush bristles against the bottom of the container, ③ withdrawal of the brush, or ① penetration of the brush bristles into the sputum specimen, ② withdrawal of the brush, and application of the specimen to the carrier after the sampling process is complete.

4. The utility model relates to a sampling smear application of a clinical test sputum specimen of a brush, which is characterized in that the utility model is used for the sampling smear application of the sputum specimen of tuberculosis patients.

5. The method according to claim 1 or 2, wherein the brush is mainly composed of a shaft 1 and bristles 2, and the bristles 2 have a length of 3 to 20mm and a diameter of 0.05 to 0.4 mm.

6. The method according to claim 3, wherein the brush is mainly composed of a shaft 1 and bristles 2, and the bristles 2 have a length of 3 to 20mm and a diameter of 0.05 to 0.4 mm.

7. The use according to claim 4, wherein the brush is mainly composed of a shaft 1 and bristles 2, the bristles 2 having a length of 3 to 20mm and a diameter of 0.05 to 0.4 mm.

8. The method according to claim 1 or 2, wherein the brush is mainly composed of a shaft 1 and bristles 2, the bristles 2 are made of a metal material and have a length of 3 to 20mm and a diameter of 0.05 to 0.13 mm; or the material of the brush hair 2 is plastic material, the length is 3-20 mm and the diameter is 0.13-0.4 mm.

9. The method according to claim 3, wherein the brush is mainly composed of a shaft 1 and bristles 2, the bristles 2 are made of a metal material and have a length of 3 to 20mm and a diameter of 0.05 to 0.13 mm; or the material of the brush hair 2 is plastic material, the length is 3-20 mm and the diameter is 0.13-0.4 mm.

10. The use according to claim 4, wherein the brush is mainly composed of a shaft 1 and bristles 2, the bristles 2 are made of a metal material and have a length of 3 to 20mm and a diameter of 0.05 to 0.13 mm; or the material of the brush hair 2 is plastic material, the length is 3-20 mm and the diameter is 0.13-0.4 mm.