CN111359305B - Preparation method and device of hyaluronic acid with moisturizing effect for skin care - Google Patents

Abstract

The embodiment of the invention discloses a preparation device of hyaluronic acid with moisturizing effect for skin care, which comprises a filter cover, wherein an inclined ring is arranged on the inner wall of the bottom of the filter cover, a material guide platform with an isosceles trapezoid-shaped cross section is arranged at the central position of the bottom of the filter cover, a filter plate is arranged at one end, away from the filter cover, of the material guide platform, a filter material mechanism is arranged on the outer wall of the bottom of the filter cover, a standing separation mechanism connected with the filter cover is arranged below the filter material mechanism, the device can enable a solution to be pushed by gas when passing through a discharge hole through a gas guide cavity and then pass through a filter element at a higher speed, and then the solution is atomized by a filter material atomizer after primary filtration of the filter element, so that the solution can flow faster and then is filtered again by the filter element, and even if the filter element fails, and the filter element does not need to be frequently replaced due to the existence of the filter bag, so that the difficulty of operation is reduced.

Description

Preparation method and device of hyaluronic acid with moisturizing effect for skin care

Technical Field

The embodiment of the invention relates to the field of preparation of hyaluronic acid, in particular to a preparation method and a device of hyaluronic acid with a moisturizing effect for skin care.

Background

Hyaluronic acid has biochemical drugs with high clinical value, and is widely applied to various ophthalmic surgeries, such as crystal implantation, corneal transplantation, anti-glaucoma surgeries and the like. It can also be used for treating arthritis and promoting wound healing. The cockscomb skin-protecting cosmetic has unique skin-protecting effect, can keep skin moist, smooth, fine, tender and elastic, has the effects of preventing wrinkle, resisting wrinkle, beautifying, protecting health and recovering skin physiological functions, is mostly prepared by taking the cockscomb as a raw material during preparation, and is subjected to a series of operations such as drying, filtering and the like.

Present cockscomb raw materials are ground and soaked the back, and its liquid viscosity that obtains is great, and the speed through the filtration pore is slower when filtering its filtration efficiency is slower, and in case meet a large amount of raw materials and need carry out filtering operation, just need filter with the help of a plurality of equipment or a plurality of filter cores (like active carbon etc.), and because of filterable raw materials is more, need the frequent change filter core of user just can continue to filter purification operation, and its operation is comparatively loaded down with trivial details complicated.

Disclosure of Invention

Therefore, the embodiment of the invention provides a preparation method and a device of hyaluronic acid with moisturizing effect for skin care, so as to solve the problems that in the prior art, due to high viscosity of liquid, the speed of passing through a filter hole during filtering is low, namely the filtering efficiency is low, once a large amount of raw materials need to be filtered, multiple devices or multiple filter elements (such as activated carbon and the like) are needed for filtering, and due to more raw materials to be filtered, a user needs to frequently replace the filter elements to continue filtering and purifying operations, so that the operation is complicated.

In order to achieve the above object, an embodiment of the present invention provides the following:

the utility model provides a preparation facilities of hyaluronic acid that possesses effect of moisturizing for skin nursing, includes the filter mantle, install the slope ring at the bottom inner wall of filter mantle, and the central point department of filter mantle bottom installs the guide platform of transversal isosceles trapezoid structure, the filter plate is installed to the one end that the filter mantle was kept away from to the guide platform, the filter material mechanism is installed to the bottom outer wall of filter mantle, be equipped with the separating mechanism that stews who is connected with the filter mantle below the filter material mechanism, offer the relief hole that is used for arranging the material and gets into in the filter material mechanism at the lower surface of filter mantle with the marginal position department of guide platform, and be provided with the air guide chamber that communicates with the relief hole and be used for leading-in gas at the filter mantle lateral wall;

filter material mechanism includes tip and relief hole sealing connection's a filter core cavity install the transversal filter material atomizer who personally submits the font structure in the filter core cavity a filter material atomizer installs the filter core in the filter material atomizer, the one end that filter mantle was kept away from to the filter core cavity a filter bag's outside cover is equipped with the second filter bag of being connected with a filter core cavity section of thick bamboo.

As a preferable aspect of the present invention, the diameter of the filter hole of the first filter bag is smaller than the diameter of the filter hole of the second filter bag, and the length of the first filter bag is smaller than the length of the second filter bag.

In a preferred embodiment of the present invention, the discharge holes include a first row of holes connected to an edge of the material guide table, an inclined hole hermetically connected to the hollow cylinder of the filter element is provided below the first row of holes, and an air guide block having a C-shaped cross section and corresponding to the discharge holes is provided on an inner wall of the inclined hole.

As a preferable scheme of the invention, the filter plate is composed of a main filter plate connected with the inner wall of the filter cover, a plurality of main filter holes are arranged on the surface of the main filter plate, and a filter boss is arranged at the position of the surface of the main filter plate corresponding to the main filter holes.

As a preferable scheme of the invention, the standing separation mechanism comprises a standing cover connected with the bottom of the filter cover, a flow guide ring is installed on the inner wall of the bottom of the standing cover, a separation funnel is installed on the outer wall of the bottom of the standing cover, a flow guide hole is formed in the position, corresponding to the separation funnel, of the surface of the standing cover, a flow rate regulator is connected to the inner wall of the separation funnel, and an auxiliary liquid taking device is installed on the side wall of the separation funnel.

As a preferable scheme of the invention, the separating funnel consists of a main funnel and a separating funnel, wherein one end of the main funnel is hermetically connected with the bottom of the standing cover, the separating funnel is arranged at the other end of the main funnel, and a liquid flowing hole communicated with the main funnel is formed in the separating funnel.

As a preferable scheme of the invention, the flow rate regulator comprises a plurality of arc-shaped covers arranged at the bottoms of the standing covers, a rotating column is connected in each arc-shaped cover, rotating columns connected with the inner wall of the main funnel are respectively arranged at two ends of each rotating column, a flow-out hole is arranged on each rotating column in a penetrating manner, a first groove is arranged at one end of each flow-out hole, a second groove is arranged at the other end of each flow-out hole, an adjusting cover with a V-shaped cross section is connected to the inner wall of each second groove, and a fine hole column extending into the first groove is connected to the surface of one side, close to the flow-out hole, of each adjusting cover.

As a preferable scheme of the invention, the auxiliary liquid taking device comprises an auxiliary cover mounted on the side wall of the separating funnel and a threaded column penetrating the auxiliary cover, two liquid guide blocks with right-angled trapezoid cross sections are symmetrically mounted on the inner wall of the auxiliary cover, a liquid blocking block connected with the auxiliary cover and used for guiding liquid in a liquid flow hole into the auxiliary cover is arranged between the two liquid guide blocks, the cross section of the liquid blocking block is in an L-shaped structure, the threaded column is positioned right above the liquid guide block, and one end of the threaded column close to the adjusting cover is connected with the side wall of the liquid blocking block.

A preparation method of hyaluronic acid with moisturizing effect for skin care comprises the following steps:

s100, adding the cockscomb fragments into acetone, and soaking overnight until the cockscombs are dehydrated and hardened. Then placing the cockscomb powder in an enamel tray for drying, crushing the cockscomb powder into powder by a crusher after drying, pouring the cockscomb powder into an enamel tank, adding 6-7 times of distilled water, stirring uniformly, and soaking for more than 24 hours to fully swell the cockscomb powder;

s200, pouring the swollen cockscomb powder into a filter cover, and introducing gas to fully filter the solution under the action of high pressure;

s300, introducing the filtered filtrate into a standing cover, adding 10% solid sodium chloride, then adding chloroform with the same volume, stirring for 3 hours, separating an upper aqueous phase and a lower aqueous phase through a separating funnel, adding 95% ethanol with 2 times of volume of the separated aqueous phases, uniformly stirring, standing overnight, precipitating hyaluronic acid, filtering out precipitates, and drying to obtain a crude product.

In a preferred embodiment of the present invention, the solution is fully filtered under the action of high pressure in step S200, and the specific steps are as follows:

s201, introducing the filtrate into the liquid discharge hole, simultaneously introducing high-pressure gas to enable the filtrate to pass through the filter element at a high speed, and then atomizing the filtrate primarily filtered by the filter element by using an atomizer, so that the filtrate is filtered again by the filter bag, and then the filtrate without impurities can be obtained.

The embodiment of the invention has the following advantages:

the equipment enables the solution to be pushed by gas when passing through the discharge hole through the gas guide cavity and the two filter bags, so that the solution can pass through the filter element at a higher speed, then is atomized by the filter material atomizer after being primarily filtered by the filter element, so that the solution can flow faster, and then is filtered again by the filter bags, so that the filtering efficiency is higher, and even if the filter element fails, the filter element does not need to be frequently replaced due to the existence of the filter bags, thereby reducing the difficulty of operation;

and this equipment accessible cover that stews realizes the purpose of separating upper and lower floor solution, and the accessible rotates the separation rate that the post is used for controlling solution simultaneously and avoids the velocity of flow very fast, thereby leads to the upper and lower floor to flow the condition that the mixing appears together easily, and still can make the solution of different layers flow from different flow passages through keeping off the liquid piece, and the condition that can not appear mixing once more (because of solution thickness is great in the flow passage extremely easily attached solution).

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.

FIG. 1 is a schematic overall structure diagram of an embodiment of the present invention;

FIG. 2 is a schematic top cross-sectional view of an auxiliary shield according to an embodiment of the present invention;

FIG. 3 is a schematic cross-sectional front view of a rotary column according to an embodiment of the present invention;

FIG. 4 is a side view of a rotating column of an embodiment of the present invention.

In the figure:

1-a filter mantle; 2-an inclined ring; 3-a material guiding platform; 4-filter plate; 5, a filter material mechanism; 6-standing and separating mechanism; 7-a discharge hole; 8-a gas conducting cavity;

401-main filter plate; 402-main filtration pore; 403-a filtration boss;

501-hollow cylinder of filter element; 502-filter media atomizer; 503-a filter element; 504-a first filter bag; 505-a second filter bag;

601-a standing cover; 602-a flow guide ring; 603-a separating funnel; 604-guiding holes; 605-flow rate regulator; 606-auxiliary liquid taking device; 607-a main funnel; 608-separating funnel; 609-liquid flow holes; 610-arc cover; 611-rotating column; 612-spin columns; 613-outflow holes; 614-first groove; 615-a second groove; 616-adjustment cover; 617-fine pore column; 618-auxiliary cover; 619-threaded post; 620-liquid guide block; 621-liquid blocking block;

701-a first row of holes; 702-an inclined hole; 703-gas guiding block.

Detailed Description

The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

As shown in FIG. 1, the present invention provides a device for preparing hyaluronic acid with moisturizing effect for skin care, which can pass through the air guide cavity 8 and two filter bags, so that the solution can be pushed by air when passing through the discharge hole 7, so as to pass through the filter element 503 at a higher speed, then is primarily filtered by the filter element 503 and then is atomized by the filter material atomizer, then the atomized filtrate is filtered by a filter bag to ensure that the purity of the finished product is higher, and simultaneously the aim of separating the upper layer solution from the lower layer solution can be realized by the standing cover 601, meanwhile, the separation speed of the solution can be controlled by the rotating column 611 to avoid the situation that the solution flows together to cause mixing of the upper layer and the lower layer due to the fact that the flow speed of the solution is high, and the solution in different layers can flow out from different flow passages through the liquid blocking block 621, so that the solution cannot be mixed again (the solution is easy to adhere to the flow passages due to the high solution consistency).

In this example, crude hyaluronic acid can be directly produced by the production apparatus, and then the operation of refining the crude hyaluronic acid is the same as the conventional synthesis operation (the apparatus is the same as the conventional production method of hyaluronic acid, but the efficiency of production is higher by the apparatus).

As shown in fig. 1, including filter mantle 1 the bottom inner wall of filter mantle 1 installs tilt ring 2, and the concrete structure of this tilt ring 2 can refer to fig. 1, and the thickness of this tilt ring 2 sets up with linear degressive trend from supreme down, so can make the condition that the solution can all be discharged through relief hole 7, and the central point department of filter mantle 1 bottom installs the transversal guide platform 3 that personally submits isosceles trapezoid structure, and this guide platform 3 cooperates with tilt ring 2 and can make the solution that gets into in filter mantle 1 always be located between guide platform 3 and tilt ring 2 to all get into in the relief hole 7, the filter plate 4 is installed to the one end that filter mantle 1 was kept away from to guide platform 3, filter material mechanism 5 is installed to the bottom outer wall of filter mantle 1 the below of filter material mechanism 5 is equipped with the separator 6 that stews that is connected with filter mantle 1.

When the device is used, a swollen cockscomb powder solution can be directly poured into a filter cover 1, then the solution is primarily filtered by a filter plate 4 and flows between a material guide table 3 and an inclined ring 2, then the solution enters a filter material mechanism 5, then the solution is filtered by the filter material mechanism 5 and drops into a standing separation mechanism 6, then 10% of solid sodium chloride is added into the solution in the standing separation mechanism 6, then chloroform with the same volume is added, after stirring for 3 hours, upper and lower water phases are separated by the standing separation mechanism 6, then 95% ethanol with 2 times of volume is added into the separated water phases, the mixture is uniformly stirred, standing overnight is carried out, hyaluronic acid is precipitated, then precipitates are filtered, and a crude product is obtained after drying.

The lower surface of filter mantle 1 with the border position department of guide platform 3 offers the relief hole 7 that is used for arranging the material and gets into in the filter material mechanism 5, just filter mantle 1 lateral wall is provided with and communicates with relief hole 7 and be used for leading-in gaseous air guide chamber 8, and this relief hole 7 is used for carrying out filtering operation in the leading-in filter material mechanism 5 of material that will dissolve, and air guide chamber 8 is used for leading-in relief hole 7 with external high-pressure gas, makes the filtration efficiency of solution higher, and this air guide chamber 8's concrete structure is as shown in fig. 1.

As shown in fig. 1, filter material mechanism 5 includes a filter core hollow cylinder 501 of tip and relief hole 7 sealing connection install the filter material atomizer 502 of transversal personally submitting the font structure in the filter core hollow cylinder 501 filter material atomizer 503 is installed in the filter material atomizer 502, first filter bag 504 is installed to the one end that filter mantle 1 was kept away from to filter core hollow cylinder 501 the outside cover of first filter bag 504 is equipped with the second filter bag 505 of being connected with filter core hollow cylinder 501, the filtration pore diameter of first filter bag 504 is less than the filtration pore diameter of second filter bag 505, just the length of first filter bag 504 is less than the length of second filter bag 505, so set up two filter bags in order to make filterable efficiency higher, and common cloth filter bag on the filter bag selectable market.

When this filter material mechanism 5 filters, in case gaseous promotion solution gets into the hollow section of thick bamboo 501 back of filter core, can be filtered by filter core 503 earlier, it is very fast to get into the solution speed in the hollow section of thick bamboo 501 of filter core simultaneously, even liquid viscosity is great, its filtration efficiency also can not be too slow, later the solution after being filtered by filter core 503 can be atomized by filter material atomizer 502, this filter material atomizer 502's atomizing hole as long as satisfy can not blockked up by the debris in the solution can, later by the liquid after the atomizing can be filtered by first filter bag 504, then filtered by second filter bag 505, later by fully filterable filtrating can assemble in the separating mechanism 6 that stews.

As shown in fig. 1, the discharge hole 7 includes a first discharge hole 701 connected to an edge of the material guide table 3, an inclined hole 702 hermetically connected to the hollow cylinder 501 of the filter element is provided below the first discharge hole 701, and an air guide block 703 having a C-shaped cross section and corresponding to the discharge hole 7 is attached to an inner wall of the inclined hole 702.

The specific process that the gas pushes the solution to flow is that once the solution is between the material guide table 3 and the inclined ring 2, the solution enters the inclined hole 702 through the first row of holes 701, the inclined hole 702 inclines towards the direction of the hollow cylinder 501 of the filter element, at the moment, once the external high-pressure gas enters the inclined hole 702 through the gas guide cavity 8, the external high-pressure gas directly impacts the gas guide block 703, the cross section of the gas guide block 703 is of a C-shaped structure, so that the solution impacting on the gas guide block 703 can be driven by the gas to move along the inner arc surface of the gas guide block 703, directly enters the hollow cylinder 501 of the filter element, and the speed of the solution entering the hollow cylinder 501 of the filter element is higher, so that the aim of controlling the flow rate of the solution is achieved, and.

As shown in fig. 1, the filter plate 4 is composed of a main filter plate 401 connected to the inner wall of the filter mantle 1, a plurality of main filter holes 402 are formed in the surface of the main filter plate 401, and a filter boss 403 is installed on the surface of the main filter plate 401 and at a position corresponding to the main filter holes 402, so that after a solution is poured into the filter mantle 1, the solution is filtered by the filter boss 403 on the main filter plate 401, and the solution drops between the material guide table 3 and the inclined ring 2, and the filter residue is blocked by the filter boss 403 without blocking the first row of holes 701.

In this embodiment, except for the device directly used for filtration, such as a filter bag, all other components are made of a glass transparent material, so that a user can observe layering and perform discharge operation conveniently.

As shown in fig. 1, the standing separation mechanism 6 includes a standing cover 601 connected to the bottom of the filter cover 1, a membrane bag is disposed on a side wall of the standing cover 601, so that the charged gas can be discharged normally, a flow guide ring 602 is installed on an inner wall of the bottom of the standing cover 601, a separation funnel 603 is installed on an outer wall of the bottom of the standing cover 601, a flow guide hole 604 is opened on a position of the surface of the standing cover 601 corresponding to the separation funnel 603, a flow rate regulator 605 is connected to an inner wall of the separation funnel 603, and an auxiliary liquid taking device 606 is installed on a side wall of the separation funnel 603.

When the filtered solution entering the standing separation mechanism 6 is directly gathered at the bottom of the standing cover 601 (because of the existence of the guide ring 602, the filtered solution is not distributed at the bottom of the whole standing cover 601 but is gathered together by the guide ring 602), then the filtered filtrate is introduced into the standing cover 601 and 10% solid sodium chloride is added, then chloroform with the same volume is added, after 3 hours of stirring, upper and lower aqueous phases are separated by the separation funnel 603 (the flow rate of separation can be controlled by the flow rate regulator 605 during separation, the situation that the upper and lower layers flow together to cause mixing is avoided, and the solutions in different layers flow out from different flow channels through the liquid baffle 621 without mixing again (because the solution is very easy to adhere to the flow channel with larger consistency) is avoided), 95% ethanol with 2 times of volume is added into the separated aqueous phases, and the stirring is uniform, standing overnight to precipitate hyaluronic acid, filtering to remove precipitate, and drying to obtain crude product.

The separating funnel 603 is composed of a main funnel 607 with one end hermetically connected with the bottom of the standing cover 601 and a separating funnel 608 arranged at the other end of the main funnel 607, a liquid flow hole 609 communicated with the main funnel 607 is arranged in the separating funnel 608, the separating funnel 603 is arranged in such a way that the liquid can be normally separated during liquid separation, and the liquid flow hole 609 has a small aperture and cannot flow at a fast flow rate.

As shown in fig. 1, 3 and 4, the flow rate regulator 605 includes a plurality of arc-shaped covers 610 installed at the bottom of the stationary cover 601, a rotating column 611 is connected in the arc-shaped covers 610, rotating columns 612 connected to the inner wall of the main funnel 607 are installed at both ends of the rotating column 611, an outflow hole 613 is penetratingly provided on the rotating column 611, a first groove 614 is provided at one end of the outflow hole 613, a second groove 615 is provided at the other end of the outflow hole 613, a regulating cover 616 having a V-shaped cross section is connected to the inner wall of the second groove 615, and a fine hole column 617 extending into the first groove 614 is connected to one side surface of the regulating cover 616 near the outflow hole 613.

The detailed structure of the fine hole column 617 is shown in fig. 3, and the size of the adjusting cap 616 is smaller than the size of the second groove 615, and the sidewall of the adjusting cap 616 is installed with a connection column connected to the inner wall of the second groove 615, so that the adjusting cap 616 cannot move easily, and then the solution can flow normally through the outflow hole 613 and then flow out through the second groove 615, where the flow rate is the largest and the flow rate is the smallest when the solution flows out through the adjusting cap 616.

When the liquid in the stationary cover 601 is separated, the flow rate regulator 605 controls the flow rate, and the specific operation is that once the user needs to discharge the solution at the bottom of the arc-shaped cover 610 at a larger flow rate, only the rotating column 612 is rotated to move the rotating column 611 with it, then the rotating column 611 is rotated clockwise to align the first notch 614 with the diversion hole 604, then the solution flows into the outflow hole 613 directly through the first notch 614 until flowing out of the second notch 615, then when the flow needs to flow at a smaller speed, only the rotating column 612 is rotated in the opposite direction to rotate the rotating column 611 in the counterclockwise direction until the regulating cover 616 is aligned with the diversion hole 604, then the solution flows into the fine-hole column 617 directly through the regulating cover 616, and finally flows out of the end of the fine-hole column 617, and the flow speed is smaller.

In this embodiment, in order to block the solution in the diversion hole 604 by the rotating column 611, the rubber sleeve is directly sleeved on the sidewall of the rotating column 611, so that the solution can flow normally, and a stop table can be disposed on the sidewall of the rubber sleeve, so that the rotating column 611 cannot move easily when rotating to a proper position.

As shown in fig. 1 and 2, the auxiliary liquid taking device 606 includes an auxiliary cover 618 mounted on a side wall of the separating funnel 608, and a threaded post 619 disposed on the auxiliary cover 618 in a penetrating manner, two liquid guide blocks 620 having right-angle trapezoidal cross sections are symmetrically mounted on an inner wall of the auxiliary cover 618, a liquid blocking block 621 connected to the auxiliary cover 618 and used for guiding liquid in the liquid flow hole 609 into the auxiliary cover 618 is disposed between the two liquid guide blocks 620, the cross section of the liquid blocking block 621 has an L-shaped structure, the threaded post 619 is located right above the liquid guide block 620, and one end of the threaded post 619 close to the adjusting cover 616 is connected to a side wall of the liquid blocking block 621.

When the solution flows, because the solution is very easy to adhere to the flow passages (the flow holes 609) with a large solution consistency, once all the solution flows out of one flow passage, the mixture is very easy to mix, so the solutions of different layers can flow out of different flow passages through the auxiliary liquid taking device 606, and the situation of mixing again can not occur, when the solution flows into the flow holes 609, once the solution flows into the flow holes 609, a user can rotate the threaded column 619 to push the liquid blocking block 621 to slide into the flow holes 609 until the liquid blocking block 621 is fully attached to the inner wall of the flow holes 609 (for better attachment, a sealing embedded groove for accommodating the liquid blocking block 621 can be arranged on the inner wall of the flow holes 609) (the size of the flow holes 609 and the liquid blocking block 621 is as shown in figure 2, the solution can directly enter the auxiliary cover 618 only if the size is so arranged), and then the solution can flow out from the gap between the liquid blocking block 621 and the liquid guiding block 620 through the guidance of the liquid blocking block 621, until the solution flows into the storage bottle, after all the solution in the first layer flows out, the threaded column 619 can be rotated in the opposite direction, so that the liquid blocking block 621 slides to the position between the two liquid guiding blocks 620, and the solution in the second layer can only flow away through the liquid flowing hole 609 but cannot flow away from the liquid guiding blocks 620.

A preparation method of hyaluronic acid with moisturizing effect for skin care comprises the following steps:

s100, adding the cockscomb fragments into acetone, and soaking overnight until the cockscombs are dehydrated and hardened. Then placing the cockscomb powder in an enamel tray for drying, crushing the cockscomb powder into powder by a crusher after drying, pouring the cockscomb powder into an enamel tank, adding 6-7 times of distilled water, stirring uniformly, and soaking for more than 24 hours to fully swell the cockscomb powder;

s200, pouring the swollen cockscomb powder into a filter cover, and introducing gas to fully filter the solution under the action of high pressure;

s300, introducing the filtered filtrate into a standing cover, adding 10% solid sodium chloride, then adding chloroform with the same volume, stirring for 3 hours, separating an upper aqueous phase and a lower aqueous phase through a separating funnel, adding 95% ethanol with 2 times of volume of the separated aqueous phases, uniformly stirring, standing overnight, precipitating hyaluronic acid, filtering out precipitates, and drying to obtain a crude product.

After the crude product is made, it can be further refined to give the finished product: dissolving the crude product in a 4-time volume of 0.1mol/L sodium chloride solution, adjusting the pH value to 4.5-5 by using dilute hydrochloric acid, adding equal volume of chloroform, standing for layering, and sucking out an upper aqueous phase. The aqueous phase was adjusted to pH 7.5 with 8% sodium hydroxide solution, pronase was added and incubated at 37 ℃ for 24 h. Extracting with equal volume of chloroform for 2 times, mixing the upper water phase and the lower liquid phase, and recovering chloroform. Adding 1% cetylpyridinium chloride solution with the same volume into the water phase, stirring uniformly, standing for precipitation, filtering, and collecting the precipitate. Dissolving the precipitate with 2-3 times of 0.4mol/L sodium chloride, filtering, adding 3 times of 95% ethanol into the filtrate, standing for precipitation, removing the upper ethanol layer, repeatedly precipitating the lower layer with 95% ethanol for 2 times, suspending and filtering the lower precipitate, drying, washing with acetone for 2 times, and vacuum drying to obtain the final product.

In step S200, the solution is fully filtered under the action of high pressure, and the specific steps are as follows:

s201, introducing the filtrate into the liquid discharge hole, simultaneously introducing high-pressure gas to enable the filtrate to pass through the filter element at a high speed, and then atomizing the filtrate primarily filtered by the filter element by using an atomizer, so that the filtrate is filtered again by the filter bag, and then the filtrate without impurities can be obtained.

Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (9)

1. A device for preparing hyaluronic acid with moisturizing effect for skin care is characterized by comprising a filter cover (1), an inclined ring (2) is arranged on the inner wall of the bottom of the filter cover (1), a material guide platform (3) with an isosceles trapezoid-shaped cross section is arranged at the central position of the bottom of the filter cover (1), a filter plate (4) is arranged at one end of the material guide platform (3) far away from the filter cover (1), a filter material mechanism (5) is arranged on the outer wall of the bottom of the filter cover (1), a standing separation mechanism (6) connected with the filter cover (1) is arranged below the filter material mechanism (5), a discharge hole (7) for discharging materials to enter the filtering material mechanism (5) is arranged at the edge position of the lower surface of the filtering cover (1) and the material guiding platform (3), a gas guide cavity (8) which is communicated with the discharge hole (7) and is used for guiding gas is arranged on the side wall of the filter cover (1);

the filter material mechanism (5) comprises a filter element hollow cylinder (501) with the end part hermetically connected with the discharge hole (7), a filter material atomizer (502) with the cross section in a character-shaped structure is installed in the filter element hollow cylinder (501), a filter element (503) is installed in the filter material atomizer (502), a first filter bag (504) is installed at one end, far away from the filter cover (1), of the filter element hollow cylinder (501), and a second filter bag (505) connected with the filter element hollow cylinder (501) is sleeved on the outer side of the first filter bag (504);

the bin outlet (7) by first round of hole (701) of being connected with the edge of guide platform (3) the below of first round of hole (701) is equipped with down hole (702) with hollow section of thick bamboo (501) sealing connection in the filter core the air guide piece (703) that correspond and transversal personally submit C type structure with bin outlet (7) are installed to the inner wall of down hole (702).

2. The device for preparing hyaluronic acid with moisturizing effect for skin care according to claim 1, wherein the filter pore diameter of the first filter bag (504) is smaller than that of the second filter bag (505), and the length of the first filter bag (504) is smaller than that of the second filter bag (505).

3. The device for preparing hyaluronic acid with moisturizing effect for skin care according to claim 1, wherein the filter plate (4) is composed of a main filter plate (401) connected with the inner wall of the filter cover (1), a plurality of main filter holes (402) are formed in the surface of the main filter plate (401), and a filter boss (403) is installed on the surface of the main filter plate (401) at a position corresponding to the main filter holes (402).

4. The device for preparing hyaluronic acid with moisturizing effect for skin care according to claim 1, wherein the standing and separating mechanism (6) comprises a standing cover (601) connected with the bottom of the filter cover (1), a flow guide ring (602) is mounted on the inner wall of the bottom of the standing cover (601), a separating funnel (603) is mounted on the outer wall of the bottom of the standing cover (601), a flow guide hole (604) is formed in the position, corresponding to the separating funnel (603), on the surface of the standing cover (601), a flow rate regulator (605) is connected to the inner wall of the separating funnel (603), and an auxiliary liquid taking device (606) is mounted on the side wall of the separating funnel (603).

5. The device for preparing hyaluronic acid with moisturizing effect for skin care according to claim 4, wherein the separating funnel (603) comprises a main funnel (607) with one end hermetically connected with the bottom of the standing cover (601) and a separating funnel (608) installed at the other end of the main funnel (607), and a liquid flowing hole (609) communicated with the main funnel (607) is formed in the separating funnel (608).

6. The device for preparing hyaluronic acid with moisturizing effect according to claim 4, characterized in that the flow rate regulator (605) comprises a plurality of arc-shaped covers (610) arranged at the bottom of the standing cover (601), a rotating column (611) is connected in the arc-shaped cover (610), two ends of the rotating column (611) are respectively provided with a rotating column (612) connected with the inner wall of the main funnel (607), an outflow hole (613) is arranged on the rotating column (611) in a penetrating way, a first groove (614) is arranged at one end of the outflow hole (613), and the other end of the outflow hole (613) is provided with a second groove (615), the inner wall of the second groove (615) is connected with an adjusting cover (616) with a V-shaped cross section, a fine hole column (617) extending into the first groove (614) is connected to one side surface of the adjusting cover (616) close to the outflow hole (613).

7. The device for preparing hyaluronic acid with moisturizing effect according to claim 4, characterized in that the auxiliary liquid taking device (606) comprises an auxiliary cover (618) arranged on the side wall of the separating funnel (608) and a threaded column (619) arranged on the auxiliary cover (618) in a penetrating way, two liquid guide blocks (620) with right-angle trapezoid cross sections are symmetrically arranged on the inner wall of the auxiliary cover (618), a liquid blocking block (621) which is connected with the auxiliary cover (618) and is used for guiding the liquid in the liquid flowing hole (609) into the auxiliary cover (618) is arranged between the two liquid guiding blocks (620), the cross section of the liquid blocking block (621) is of an L-shaped structure, the threaded column (619) is positioned right above the liquid guide block (620), and one end of the threaded column (619) close to the adjusting cover (616) is connected with the side wall of the liquid blocking block (621).

8. A method for preparing hyaluronic acid with moisturizing effect for skin care, which comprises the following steps of using the device for preparing hyaluronic acid with moisturizing effect for skin care according to any one of claims 1 to 7:

s100, adding the cockscomb fragments into acetone, soaking overnight until the cockscombs are dehydrated and hardened, then placing the cockscombs into an enamel tray for drying, crushing the cockscombs into powder by a crusher after drying, pouring the cockscomb powder into an enamel tank, adding 6-7 times of distilled water, stirring uniformly, and soaking for more than 24 hours to fully swell the cockscomb powder;

s200, pouring the swollen cockscomb powder into a filter cover, and introducing gas to fully filter the solution under the action of high pressure;

s300, introducing the filtered filtrate into a standing cover, adding 10% solid sodium chloride, then adding chloroform with the same volume, stirring for 3 hours, separating an upper aqueous phase and a lower aqueous phase through a separating funnel, adding 95% ethanol with 2 times of volume of the separated aqueous phases, uniformly stirring, standing overnight, precipitating hyaluronic acid, filtering out precipitates, and drying to obtain a crude product.

9. The method for preparing hyaluronic acid with moisturizing effect for skin care according to claim 8, wherein the solution is fully filtered under high pressure in step S200, and the method comprises the following specific steps:

s201, introducing the filtrate into the liquid discharge hole, simultaneously introducing high-pressure gas to enable the filtrate to pass through the filter element at a high speed, and then atomizing the filtrate primarily filtered by the filter element by using an atomizer, so that the filtrate is filtered again by the filter bag, and then the filtrate without impurities can be obtained.

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