CN111358918A - Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof - Google Patents
Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof Download PDFInfo
- Publication number
- CN111358918A CN111358918A CN202010401169.3A CN202010401169A CN111358918A CN 111358918 A CN111358918 A CN 111358918A CN 202010401169 A CN202010401169 A CN 202010401169A CN 111358918 A CN111358918 A CN 111358918A
- Authority
- CN
- China
- Prior art keywords
- curcuma
- mixing
- traditional chinese
- chinese medicine
- oral liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of aquatic products, and particularly relates to a traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome, which comprises the following components in parts by mass: 30-40 parts of extract concentrated solution obtained by mixing and extracting radix curcumae and curcuma, 10-20 parts of acanthopanax extract concentrated solution, 0.3-1 part of volatile oil obtained by mixing and extracting radix curcumae and curcuma, 2.5-3 parts of cosurfactant and 10-25 parts of surfactant; the preparation method comprises the following steps: mixing radix Curcumae and Curcuma rhizome, and extracting to obtain volatile oil and concentrated extractive solution; extracting radix Acanthopanacis Senticosi to obtain concentrated radix Acanthopanacis Senticosi extract; 3) mixing the concentrated extractive solutions obtained in step 1) and step 2), mixing with volatile oil extracted from radix Curcumae and Curcuma rhizome, cosurfactant, and surfactant, stirring thoroughly, and mixing well. The traditional Chinese medicine oral liquid not only reduces the labor cost of clinical use, but also improves the absorption and utilization of the medicine in aquatic animals.
Description
Technical Field
The invention belongs to the technical field of aquatic products, and particularly relates to a traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and a preparation method thereof.
Background
Currently, high-density and intensive aquaculture is the main mode of aquaculture in China. However, in order to save cost and obtain higher economic benefits, the aquaculture industry often adopts irregular methods and measures, such as not clearing the aquaculture water environment for a long time, blindly improving stocking density, neglecting the nutrition balance and safety and sanitation of feed, randomly applying medicines, increasing the application frequency and the like. Therefore, the internal and external environments of the cultured fishes are unbalanced, so that the health of the fishes is influenced, and the lives of the fishes are threatened. In recent years, various syndromes frequently occur in various cultured animals in aquaculture, including a series of nonpathogenic diseases such as liver and gall syndrome, stress syndrome, metabolic disorder syndrome and drug-induced syndrome of fish, shrimp, crab, soft-shelled turtle, tortoise and the like. Since most of these diseases are chronic diseases caused by cumulative injuries, early symptoms are not obvious and are easily overlooked. With the progress of the disease, symptoms such as anorexia, slow growth speed, body surface discoloration, crisp fins of scales, exophthalmos and visceral edema, discoloration, decay, congestion, bleeding and the like appear, and the pathological analysis of the pathological changes of liver and gall is obviously related to the metabolism disorder or the obstacle of the organism substances.
The health level of fish depends on the normal operation of various organs of the body, wherein the functions of liver and gallbladder are closely related to the metabolism of the fish body. The pathological researches on fish stress diseases reported in China in recent years, such as stress hemorrhagic disease, hepatobiliary syndrome, albinism, melanosis, exophthalmia and the like, show that the diseases are not related to liver and kidney damage, so that the development of a Chinese herbal medicine preparation for regulating blood, detoxifying, protecting liver and benefiting gallbladder, promoting metabolism and improving the health level of fish bodies is necessary for improving the anti-stress capability and disease resistance of fish and promoting the healthy culture of the fish.
The main points are that ① is inconvenient in practical application, the stirring and the mixing are needed, the labor force is wasted, ② the medicine can not be fully absorbed and utilized in the body due to the short digestive tract of the fish, the medicine is wasted, and the effect of the medicine can not be effectively exerted, therefore, the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome of the aquatic products and the preparation method thereof need to be designed, so as to overcome the problems.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide the traditional Chinese medicine oral liquid for preventing the aquatic hepatobiliary syndrome and the preparation method thereof, so that the problem of complicated operation in clinical practical use is well solved, and the absorption and utilization rate of the medicine in an animal body is improved.
In order to achieve the purpose, the technical scheme of the invention is a traditional Chinese medicine oral liquid for preventing aquatic hepatobiliary syndrome, which comprises the following components in parts by mass: 30-40 parts of extract concentrated solution obtained by mixing and extracting radix curcumae and curcuma, 10-20 parts of acanthopanax extract concentrated solution, 0.3-1 part of volatile oil obtained by mixing and extracting radix curcumae and curcuma, 2.5-3 parts of cosurfactant and 10-25 parts of surfactant.
Optimally, the traditional Chinese medicine oral liquid comprises the following components in parts by mass: 35 parts of extract concentrated solution of mixed extraction of curcuma aromatica and curcuma longa, 15 parts of acanthopanax extract concentrated solution, 0.5 part of volatile oil of mixed extraction of curcuma aromatica and curcuma longa, 2.8 parts of cosurfactant and 20 parts of surfactant.
Further, the extract concentrated solution of the mixed extraction of the radix curcumae and the turmeric adopts the radix curcumae and the turmeric according to the ratio of 1: 1-2, and centrifuging and concentrating the extracted extract.
Further, the cosurfactant is any one of span 80 and glycerol.
Further, the surfactant is any one of RH40, AEO-9 and NP-10.
The preparation method of the traditional Chinese medicine oral liquid for preventing the aquatic liver and gall syndrome comprises the following steps:
1) mixing radix Curcumae and Curcuma rhizome, extracting with 75% ethanol, and collecting volatile oil extracted from radix Curcumae and Curcuma rhizome; centrifuging and concentrating the extracting solution to obtain an extracting concentrated solution obtained by mixing and extracting the curcuma aromatica and the curcuma longa;
2) extracting radix Acanthopanacis Senticosi with 50% ethanol, centrifuging the extractive solution, and concentrating to obtain radix Acanthopanacis Senticosi extract concentrated solution;
3) mixing the concentrated extractive solution of radix Curcumae and Curcuma rhizome with the concentrated extractive solution of radix Acanthopanacis Senticosi to obtain concentrated extractive solution mixture;
4) mixing the volatile oil extracted from the curcuma aromatica and the curcuma longa, the cosurfactant, the surfactant and the extracted concentrated solution mixture, fully stirring and uniformly mixing to obtain the traditional Chinese medicine oral liquid.
Further, in the step 1), the ratio of the sum of the dosages of the curcuma aromatica and the curcuma longa to the dosage of the 75% ethanol is 1: 8-10.
Further, in the step 1), the extraction temperature of the mixed extraction of the curcuma aromatica and the curcuma longa is 55-65 ℃, the extraction times are 3 times, and each time lasts for 1.5 hours.
Further, in the step 2), the ratio of the using amount of the acanthopanax senticosus to the using amount of the 55% ethanol is 1: 8-10.
Further, in the step 2), the extraction temperature of the acanthopanax senticosus is 55-65 ℃, the extraction times are 2 times, and each time lasts for 2 hours.
Compared with powder, the traditional Chinese medicine oral liquid can improve the absorption and utilization rate of the medicine in the animal body, and the main reasons are as follows: 1) except for intravenous administration, dosage form factors of the medicine have great influence on the absorption of the medicine, and different oral dosage forms have different release speeds of the medicine from the preparation and have quite different absorption speeds and degrees; the bioavailability of a drug in oral dosage form is generally considered to be in the order: solution > suspension > powder > capsule > tablet > coated tablet; meanwhile, the Chinese herbal medicine powder is not easy to adhere to the granulated feed, and even after simple adhesion, a lot of Chinese herbal medicine particles are lost into water when the Chinese herbal medicine powder is put into water, so that medicine waste is caused, and the using effect of the medicine is reduced; 2) compared with livestock and poultry, intestinal tracts of fish and shrimp are generally shorter and excreted quickly, so that the dissolution and absorption time of effective components of the traditional Chinese medicine powder in the intestinal tracts of fish and shrimp can be correspondingly reduced, the absorption of the effective components is too little, the loss is too large, and the biological utilization of the medicine in animal bodies is influenced; the traditional Chinese medicine oral liquid is a product prepared by extracting effective components through a proper extraction process by special traditional Chinese medicine extraction equipment, concentrating and preparing a preparation means, and can greatly improve the absorption and utilization of the effective components by aquatic animals, thereby improving the biological utilization of the medicine in the bodies of the animals.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, 75% alcohol extraction is carried out on curcuma aromatica and curcuma, 55% alcohol extraction is carried out on acanthopanax senticosus, volatile oil in mixed extraction of curcuma aromatica and curcuma aromatica is collected by utilizing a condensation reflux technology, then an extracting solution is separated and concentrated to obtain an extraction concentrated solution, and then the volatile oil in mixed extraction of the curcuma aromatica and curcuma aromatica is added into the extraction concentrated solution by adopting an oil-in-water preparation process to prepare the traditional Chinese medicine oral liquid; the prepared oral liquid not only reduces the labor cost of clinical use, but also improves the absorption and utilization of the medicine in the aquatic animal body.
Drawings
FIG. 1 is a photograph comparing liver tissue sections of healthy grass carp (left) and grass carp suspected of having fatty liver (right).
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome, which comprises the following components in parts by mass: 35 parts of extract concentrated solution obtained by mixing and extracting radix curcumae and curcuma, 15 parts of acanthopanax extract concentrated solution, 0.5 part of volatile oil obtained by mixing and extracting radix curcumae and curcuma, 802.8 parts of span and 20 parts of fatty alcohol-polyoxyethylene ether (AEO-9).
The preparation method of the traditional Chinese medicine oral liquid comprises the following steps:
1) mixing radix Curcumae and Curcuma rhizome according to the ratio of 1: 1, extracting with 75% ethanol at 65 deg.C (ratio of medicinal liquid to medicinal liquid is 1: 10) for 3 times (each time for 1.5 hr), and collecting volatile oil extracted from radix Curcumae and Curcuma rhizome; centrifuging and concentrating the extracting solution to obtain an extracting concentrated solution obtained by mixing and extracting the curcuma aromatica and the curcuma longa;
2) extracting radix Acanthopanacis Senticosi with 55% ethanol at 65 deg.C for 2 times (2 hr each time), centrifuging the extractive solution, and concentrating to obtain concentrated radix Acanthopanacis Senticosi extract;
3) mixing the concentrated extractive solution of radix Curcumae and Curcuma rhizome with the concentrated extractive solution of radix Acanthopanacis Senticosi to obtain concentrated extractive solution mixture;
4) mixing the volatile oil extracted from the curcuma aromatica and the curcuma longa, span 80, AEO-9 and the mixture of the extracted concentrated solution, fully stirring and uniformly mixing to obtain the traditional Chinese medicine oral liquid.
Example 2
The embodiment provides a traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome, which comprises the following components in parts by mass: 30 parts of extract concentrated solution obtained by mixing and extracting curcuma aromatica and curcuma longa, 10 parts of acanthopanax root extract concentrated solution, 0.3 part of volatile oil obtained by mixing and extracting curcuma aromatica and curcuma longa, 2.5 parts of glycerol and 10 parts of nonylphenol polyoxyethylene ether (NP-10).
The preparation method of the traditional Chinese medicine oral liquid comprises the following steps:
1) mixing radix Curcumae and Curcuma rhizome according to the ratio of 1: 1 extracting with 75% ethanol at 55 deg.C for 3 times (1.5 hr each time), and collecting volatile oil extracted from radix Curcumae and Curcuma rhizome; centrifuging and concentrating the extracting solution to obtain an extracting concentrated solution obtained by mixing and extracting the curcuma aromatica and the curcuma longa;
2) extracting radix Acanthopanacis Senticosi with 55% ethanol at 55 deg.C for 2 times (2 hr each time), centrifuging the extractive solution, and concentrating to obtain concentrated radix Acanthopanacis Senticosi extract;
3) mixing the concentrated extractive solution of radix Curcumae and Curcuma rhizome with the concentrated extractive solution of radix Acanthopanacis Senticosi to obtain concentrated extractive solution mixture;
4) mixing the volatile oil extracted from the curcuma aromatica and the curcuma longa, the glycerol, the NP-10 and the extracted concentrated solution mixture, fully stirring and uniformly mixing to obtain the traditional Chinese medicine oral liquid.
Example 3
The embodiment provides a traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome, which comprises the following components in parts by mass: 40 parts of extract concentrated solution of mixed extraction of radix curcumae and curcuma, 20 parts of acanthopanax extract concentrated solution, 1 part of volatile oil of mixed extraction of radix curcumae and curcuma, 803 parts of span and 25 parts of polyoxyethylene hydrogenated castor oil (RH 40).
The preparation method of the traditional Chinese medicine oral liquid comprises the following steps:
1) mixing radix Curcumae and Curcuma rhizome according to the ratio of 1: 2 extracting with 75% ethanol at 60 deg.C (ratio of medicinal liquid to medicinal liquid is 1: 10) for 3 times (each time for 1.5 hr), and collecting volatile oil extracted from radix Curcumae and Curcuma rhizome; centrifuging and concentrating the extracting solution to obtain an extracting concentrated solution obtained by mixing and extracting the curcuma aromatica and the curcuma longa;
2) extracting radix Acanthopanacis Senticosi with 55% ethanol at 60 deg.C for 2 times (2 hr each time), centrifuging the extractive solution, and concentrating to obtain concentrated radix Acanthopanacis Senticosi extract;
3) mixing the concentrated extractive solution of radix Curcumae and Curcuma rhizome with the concentrated extractive solution of radix Acanthopanacis Senticosi to obtain concentrated extractive solution mixture;
4) mixing the volatile oil extracted from the curcuma aromatica and the curcuma longa, span 80, RH40 and the mixture of the extracted concentrated solution, fully stirring and uniformly mixing to obtain the traditional Chinese medicine oral liquid.
The following clinical application effect tests were conducted on the Chinese medicinal oral liquid of the present invention
1. Test protocol and test materials
1.1 test site and test Fish
The test site comprises: the Wuhan city new continent district Yang logical new Tokyo bay, Wei surname raiser; ponds with 4 mouths and similar sizes to the culture varieties are selected as test ponds, and the pond sizes are respectively about 8 mu (pond A), 10 mu (pond B), 8 mu (pond C) and 6 mu (pond D). Breeding 700-900 grass carps per mu, and polyculturing a small amount of silver carps and bighead carps; the grass carp is 250-650 g, and most of the grass carp is about 400 g. No obvious phenomena of diseased and dead fish before and during the test are observed.
1.2 test drugs and feeds
Test drugs: the traditional Chinese medicine oral liquid provided by the embodiment 1 of the invention is produced and trial-produced by Wuhan Huayang animal pharmaceutical industry Limited liability company;
positive control drug 1: golden yellow liver-soothing stress powder (prepared by baking and crushing curcuma aromatica, curcuma longa, radix paeoniae alba, acanthopanax senticosus and schisandra chinensis respectively and then mixing), produced by Wuhan Huayang animal pharmaceutical industry Limited liability company, with the production batch number of 20190125 and the specification of 150 g/bag;
positive control drug 2: gandanlikang powder, Wuhanzhongbo aquatic product biotechnology Limited, production batch number: 20190303, the specification is 500 g/bag;
the basic feed is a grass carp feed which is produced by Hubei sea feed Limited and is not added with any pharmaceutical additive.
1.3 method of administering drugs
Feeding the pond A with 0.05ml of traditional Chinese medicine oral liquid (provided by the embodiment 1 of the invention) per kilogram of fish weight per day as a traditional Chinese medicine oral liquid group; feeding a positive control medicament 1 (golden liver-soothing stress powder) to the pond B, and feeding 0.1g of the positive control medicament per kilogram of fish weight per day to serve as a golden liver-soothing stress powder group; feeding a positive control medicament 2 (Gandanlikang powder) to the pond C according to the specification requirement to serve as a Gandanlikang powder group; pond D was fed with conventional bait only as a blank group. The medicine is mixed with bait in certain amount every day, and the powder is mixed with bait adhesive dissolved in water and stirred.
The feeding of the test medicine and the positive control medicine is only arranged to be fed in the morning and commercial baits without medicines are fed in the afternoon every day, and the feeding is continuously carried out for 7 days.
1.4 test time and Pond conditions
The test time is 7-9 months in 2019, the pond water depth is 1.6-1.8 m, the water quality condition is general, and the transparency is about 30 cm; during the test, the water temperature is 30 +/-2 ℃, an aerator is arranged in the pond, but no new water is added, no obvious precipitation is generated, and the pH value of the pond water is about 6.5.
1.5 sample Collection
Stopping feeding for 1d after the test is finished, randomly catching 30 fishes from each pool, anesthetizing by using 200mg/L MS-222, weighing the fish body mass, collecting blood from tail veins by using a disposable syringe, and subpackaging the blood into common EDTA-K2 blood collecting tubes and EDTA-K2 blood collecting tubes added with anticoagulant, wherein the blood is respectively used for preparing serum and whole blood. Centrifuging at 4000rpm at 4 deg.C for 10min to prepare serum; dissecting to separate liver, pancreas and kidney, flushing with cold physiological saline, and removing blood from tissue; all the above operations were performed on ice and all tissue samples were stored in a-80 ℃ freezer for later use.
1.6 Main evaluation index and detection method
The disease condition and the survival rate of the test fish are observed during the test period, samples are collected on the 2 nd day after the feeding is finished, about 30 samples are taken from each test group, the relevant indexes of the grass carp are measured, and the liver protection effect of the traditional Chinese medicine oral liquid on the fish is evaluated mainly from the following two aspects.
1.6.1 measurement of hematological indices
Hematology and blood biochemistry indices include Red Blood Cells (RBC), White Blood Cells (WBC), hemoglobin (Hb), hematocrit, platelet count (PLT), alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST), Total Bilirubin (TBIL), Direct Bilirubin (DBIL), Total Protein (TP), Albumin (ALB), Globulin (GLB), urea nitrogen (BUN), blood Glucose (GLU), Cholinesterase (CHE), serum Creatinine (CR), serum Uric Acid (UA), alkaline phosphatase (AKP). Taking a whole blood sample and a serum sample, sending the whole blood sample and the serum sample to a general military Hospital in Wuhan army in Guangzhou military, wherein the whole blood sample adopts an American Abboty full-automatic hematology analyzer, and the serum sample adopts an American Beckman AU5400 full-automatic biochemical analyzer.
The test fish after blood collection was dissected to see if any abnormalities were present in their internal organs and if food was present in the gastrointestinal tract.
1.6.2 measurement of Malondialdehyde (MDA) content and superoxide dismutase (SOD) Activity in liver tissue
MDA is determined by a thiobarbituric acid (TBA) method, and the content of MDA is expressed in nmol/mg protein.
SOD activity was measured by a modified xanthine oxidase method (hydroxylamine method). The SOD activity unit is defined as: the amount of SOD corresponding to 1 activity unit (U/mg protein) when the SOD inhibition rate reaches 50% in 1ml of reaction solution per mg of tissue protein.
Two kits, namely a Malondialdehyde (MDA) determination kit (TBA method) and a total superoxide dismutase (T-SOD) test kit (hydroxylamine method), are purchased from Nanjing institute of bioengineering, and the specific determination method basically refers to the kit specification.
1.7 statistical analysis method
The experimental data were analyzed for variance, significance and correlation using the SPSS16.0 software and the excell data analysis function, and the results were expressed as mean ± standard deviation (mean ± SD).
2. Test results and discussion
2.1 general Condition observations
No significant mortality occurred in the test fish of each group during the test period; moreover, the food intake behavior is normal, the body color is normal, the physique is strong and the activity is powerful. The gastrointestinal tract is anatomically found to have food fullness of different degrees, which indicates that the drug bait is ingested by different degrees. Individual death among groups, gram-negative bacteria by bacteriology, small parasitic amount, negligible total mortality and no difference among groups.
After 2 weeks of sample collection, all test ponds were revisited and no obvious disease or death was observed, and fish grew well.
The proportion of hepatobiliary syndrome fish present among the groups was examined by dissection. The following symptoms were defined as hepatobiliary syndrome: swollen, yellow, whitish, brittle liver or partly green block-shaped liver becomes a flower liver.
2.2 examination of the apparent status of liver and pancreas
TABLE 1 proportions of test fish with different degrees of hepatobiliary syndrome in each test group
As can be seen from Table 1, the ratio of hepatobiliary syndrome (6.7%) in the test group taking the oral liquid of the present invention is lower than that in the blank group (33.3%), and the positive control drug 1 (16.7%) and 2 (20%).
2.3 results of hematological and biochemical tests on blood
TABLE 2 hematological and biochemical test results for each test group
Note: in the same row data, it indicates that the group differed significantly from the blank group (P < 0.05); if no mark, the difference is not significant.
As shown in Table 2, the white blood cell number blank group is higher than that of the traditional Chinese medicine oral liquid group, but the difference is not statistically significant (P is more than 0.05); the average aspartate aminotransferase activity and triglyceride level of the traditional Chinese medicine oral liquid group are lower than those of a blank group (P is less than 0.05), which shows that the traditional Chinese medicine oral liquid has the function of protecting liver cells and reduces liver cell damage; and total cholesterol levels were also slightly lower than in the blank. The other indicators did not significantly differ among the groups, or the average values, although largely different, were statistically not significantly different due to the large variation among individuals.
2.4 Effect of test drugs on the MDA content and SOD Activity level in the liver, pancreas and Kidney tissues
TABLE 3 comparison of SOD and MDA contents in liver and pancreas of grass carp of each test group after one week of drug administration
Note: in the same row data, it indicates that the group differed significantly from the blank group (P < 0.05); if no mark, the difference is not significant.
As can be seen from Table 3, the traditional Chinese medicine oral liquid can significantly improve the SOD activity in liver, pancreas and kidney of fish, wherein the total SOD activity in liver and pancreas is significantly increased (P is less than 0.05). In particular, each experimental group can obviously reduce the level of lipid peroxidation products (MDA) in liver and pancreas (P is less than 0.05), and the level of MDA in kidney is also reduced to a certain extent. Therefore, the effect of the traditional Chinese medicine oral liquid on preventing cell membranes from being damaged by oxidation is better than that of the golden yellow liver-soothing stress powder group and the liver and gallbladder-benefiting powder group.
2.5 histological examination results
The liver, kidney and spleen of each group of test fish are histologically sliced, 10 fish in each group are supplemented by randomly selecting fish from each group except for suspected hepatobiliary syndrome fish, namely, 5 fish in each group are randomly selected from a blank group, 9 fish in a traditional Chinese medicine oral liquid group are selected, and 8 fish in a golden yellow liver-soothing stress-relieving liver-gallbladder-benefiting powder group are selected. Except for the liver, there were no obvious abnormalities in the histological structures of other tissues and organs. The left panel in fig. 1 is liver histology of healthy fish, showing normal morphology of hepatocytes, centered nuclei, cytoplasmic homogenous red staining (left); the right panel in fig. 1 is a liver tissue section of a liver abnormal fish, and shows that liver cells are shaped like fat cells, cell nuclei move, and large lipid droplets vacuoles (right) appear in cytoplasm, which are typical characteristics of fatty liver. The number of fish with right pattern pathological changes is different in each group, 6 (60) blank groups, 1 (10) Chinese medicinal oral liquid group, 3 (30) golden yellow liver-soothing stress powder group and liver and gallbladder-benefiting powder group, and X2The number of the fishes with tissue lesions in the blank group is obviously higher than that in the traditional Chinese medicine oral liquid group (P is less than 0.05), which shows that the traditional Chinese medicine oral liquid has stronger effect of protecting the lipid degeneration of the liver cells of the fish body.
3. Summary and conclusions
The field feeding test result of the traditional Chinese medicine oral liquid group shows that the traditional Chinese medicine oral liquid group continuously feeds grass carp for 1 week according to the recommended dose (0.05ml/kg), so that the liver function condition can be effectively improved, and the proportion of 'diseased fish' with liver and gall system appearance abnormality judged as hepatogall syndrome is lower than that of a blank group without medicine; the blood index does not show any toxic or side effect of the medicine, and the medicine can obviously reduce the activity of liver transaminase, improve liver lipid metabolism and reduce the level of triglyceride in serum; in addition, the traditional Chinese medicine oral liquid obviously reduces the level of lipid peroxidation product MDA in the liver, improves the activity of antioxidant enzyme (T-SOD) in the liver, prompts the protective action and mechanism of the traditional Chinese medicine oral liquid on the liver and has a certain oxidation protective action on the kidney. Therefore, compared with the golden yellow liver-soothing stress-relieving powder and the liver and gallbladder-benefiting powder, the traditional Chinese medicine oral liquid group has stronger liver-protecting and liver-protecting effects.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome is characterized by comprising the following components in parts by mass: 30-40 parts of extract concentrated solution obtained by mixing and extracting radix curcumae and curcuma, 10-20 parts of acanthopanax extract concentrated solution, 0.3-1 part of volatile oil obtained by mixing and extracting radix curcumae and curcuma, 2.5-3 parts of cosurfactant and 10-25 parts of surfactant.
2. The traditional Chinese medicine oral liquid for preventing the aquatic hepatobiliary syndrome according to claim 1, which comprises the following components in parts by mass: 35 parts of extract concentrated solution of mixed extraction of curcuma aromatica and curcuma longa, 15 parts of acanthopanax extract concentrated solution, 0.5 part of volatile oil of mixed extraction of curcuma aromatica and curcuma longa, 2.8 parts of cosurfactant and 20 parts of surfactant.
3. The traditional Chinese medicine oral liquid for preventing the aquatic hepatobiliary syndrome according to claim 1, which is prepared by mixing the following raw materials: the concentrated extract of the mixed extraction of the radix curcumae and the turmeric is prepared by mixing the radix curcumae and the turmeric according to the weight ratio of 1: 1-2, and centrifuging and concentrating the extracted extract.
4. The traditional Chinese medicine oral liquid for preventing the aquatic hepatobiliary syndrome according to claim 1, which is prepared by mixing the following raw materials: the cosurfactant is any one of span 80 and glycerol.
5. The traditional Chinese medicine oral liquid for preventing the aquatic hepatobiliary syndrome according to claim 1, which is prepared by mixing the following raw materials: the surfactant is any one of RH40, AEO-9 and NP-10.
6. A preparation method of the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome in aquatic products according to any one of claims 1 to 5, comprising the following steps:
1) mixing radix Curcumae and Curcuma rhizome, extracting with 75% ethanol, and collecting volatile oil extracted from radix Curcumae and Curcuma rhizome; centrifuging and concentrating the extracting solution to obtain an extracting concentrated solution obtained by mixing and extracting the curcuma aromatica and the curcuma longa;
2) extracting radix Acanthopanacis Senticosi with 50% ethanol, centrifuging the extractive solution, and concentrating to obtain radix Acanthopanacis Senticosi extract concentrated solution;
3) mixing the concentrated extractive solution of radix Curcumae and Curcuma rhizome with the concentrated extractive solution of radix Acanthopanacis Senticosi to obtain concentrated extractive solution mixture;
4) mixing the volatile oil extracted from the curcuma aromatica and the curcuma longa, the cosurfactant, the surfactant and the extracted concentrated solution mixture, fully stirring and uniformly mixing to obtain the traditional Chinese medicine oral liquid.
7. The preparation method of the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome in aquatic products according to claim 6, wherein the preparation method comprises the following steps: in the step 1), the ratio of the sum of the dosages of the curcuma aromatica and the curcuma longa to the dosage of the 75% ethanol is 1: 8-10.
8. The preparation method of the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome in aquatic products according to claim 6, wherein the preparation method comprises the following steps: in the step 1), the extraction temperature of the mixed extraction of the curcuma aromatica and the curcuma longa is 55-65 ℃, the extraction times are 3 times, and each time lasts for 1.5 hours.
9. The preparation method of the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome in aquatic products according to claim 6, wherein the preparation method comprises the following steps: in the step 2), the ratio of the using amount of the acanthopanax senticosus to the using amount of the 55% ethanol is 1: 8-10.
10. The preparation method of the traditional Chinese medicine oral liquid for preventing the hepatobiliary syndrome in aquatic products according to claim 6, wherein the preparation method comprises the following steps: in the step 2), the extraction temperature of the acanthopanax senticosus is 55-65 ℃, the extraction times are 2 times, and each time lasts for 2 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010401169.3A CN111358918A (en) | 2020-05-13 | 2020-05-13 | Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010401169.3A CN111358918A (en) | 2020-05-13 | 2020-05-13 | Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111358918A true CN111358918A (en) | 2020-07-03 |
Family
ID=71200002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010401169.3A Pending CN111358918A (en) | 2020-05-13 | 2020-05-13 | Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111358918A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664540A (en) * | 2009-10-16 | 2010-03-10 | 武汉华扬动物药业有限责任公司 | Orally-taken traditional Chinese medicine preparation used for aquiculture and preparation method thereof |
-
2020
- 2020-05-13 CN CN202010401169.3A patent/CN111358918A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664540A (en) * | 2009-10-16 | 2010-03-10 | 武汉华扬动物药业有限责任公司 | Orally-taken traditional Chinese medicine preparation used for aquiculture and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zou et al. | Dietary Radix Bupleuri extracts improves hepatic lipid accumulation and immune response of hybrid grouper (Epinephelus lanceolatus♂× Epinephelus fuscoguttatus♀) | |
| Sun et al. | Effects of dietary Astragalus membranaceus (Fisch.) Bge. root extract on growth performance, plasma biochemical parameters, fish composition, liver and intestinal morphology, and genes expression in head kidney of hybrid grouper (Epinephelus lanceolatus♂× Epinephelus fuscoguttatus♀) | |
| CN105995249A (en) | Banana prawn feed additive and preparation method thereof | |
| CN104544130A (en) | Soft capsule for improving alimentary anemia and preparation method of soft capsule | |
| CN106924449A (en) | One kind prevents and treats vibriosis Chinese medicine composition and its preparation method and application | |
| CA3022247C (en) | Composition for treating diabetic disease | |
| CN103977014B (en) | A kind of medicine being applied to treat metabolic syndrome | |
| CN111358918A (en) | Traditional Chinese medicine oral liquid for preventing aquatic liver and gall syndrome and preparation method thereof | |
| JP2018070568A (en) | Nonalcoholic fatty liver disease treating or preventing agent and food for preventing nonalcoholic fatty liver disease | |
| CN113041263B (en) | Application of mussel functional lipid in preparation of products for treating type 2 diabetes | |
| Amornsakun et al. | Some aspects in early life stage of sand goby, Oxyeleotris marmoratus larvae | |
| CN115177702A (en) | Liver-protecting composition and preparation method and application thereof | |
| CN105250247A (en) | Application of astaxanthin in preparation of drugs controlling nonalcohalic fatty liver disease drugs | |
| Fang et al. | Further studies on the hepatoprotective effects of Anoectochilus formosanus | |
| CN113209062A (en) | Study on carp liver injury protection effect of curcumin through activation of activity of Nrf2 | |
| Wongtha et al. | Dietary supplementation with Limnophila aromatica extract on growth performance and physiological responses in Rana rugulosa | |
| CN110038090A (en) | A kind of compound celery oil self-emulsifying soft capsule and preparation method thereof with antigout effect | |
| Jankham et al. | Effects of nevadensin from rice paddy herb (Limnophila aromatica) on growth and physiological parameters of hybrid Catfish (Clarias macrocephalus× C. gariepinus) | |
| CN104001156A (en) | Application of eukaryotic peptide chain release factor 3b fragment 36 (eRF3b-36) in treatment of liver injury | |
| CN116874553B (en) | Preparation method and application of cockroach glycoprotein | |
| CN117338838B (en) | Traditional Chinese medicine composition and application thereof in aquatic animal antiviral drugs | |
| Abukhomra et al. | Effect of Different Doses of Sorbitol on Hematological Parameters and Blood Glucose of Rats | |
| CN107961247B (en) | Application of badger oil in preparing medicine for treating liver injury | |
| CN113171424B (en) | Traditional Chinese veterinary medicine preparation for treating liver lipid deposition of dogs and cats and preparation method thereof | |
| CN105748669B (en) | Traditional Chinese medicine composition for preventing and treating alcoholic fatty liver |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200703 |
|
| RJ01 | Rejection of invention patent application after publication |