CN111356916A - 核苷酸定序元件、芯片及定序分析方法 - Google Patents

核苷酸定序元件、芯片及定序分析方法 Download PDF

Info

Publication number
CN111356916A
CN111356916A CN201880074376.3A CN201880074376A CN111356916A CN 111356916 A CN111356916 A CN 111356916A CN 201880074376 A CN201880074376 A CN 201880074376A CN 111356916 A CN111356916 A CN 111356916A
Authority
CN
China
Prior art keywords
electrode
nucleotide sequencing
nucleotide
annular
sequencing element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201880074376.3A
Other languages
English (en)
Other versions
CN111356916B (zh
Inventor
连俊龙
萧正辉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CN111356916A publication Critical patent/CN111356916A/zh
Application granted granted Critical
Publication of CN111356916B publication Critical patent/CN111356916B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

提供一种核苷酸定序元件，包含半导体基板、晶体管、介电层、环状电极组以及导体。另提供一种核苷酸定序芯片，包含前述核苷酸定序元件与感测放大器。另提供一种定序分析的方法，包含提供如前述核苷酸定序芯片，将待测核苷酸序列片段与已知核苷酸序列片段分别置于孔槽内进行聚合反应与电化学反应，将反应所产生的电信号进行分析以获得定序结果。

Description

PCT国内申请，说明书已公开。

Claims (15)

  1. PCT国内申请，权利要求书已公开。
CN201880074376.3A 2018-09-13 2018-09-13 核苷酸定序元件、芯片及定序分析方法 Active CN111356916B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/105428 WO2020051824A1 (zh) 2018-09-13 2018-09-13 核苷酸定序元件、芯片及定序分析方法

Publications (2)

Publication Number Publication Date
CN111356916A true CN111356916A (zh) 2020-06-30
CN111356916B CN111356916B (zh) 2022-05-24

Family

ID=69777243

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880074376.3A Active CN111356916B (zh) 2018-09-13 2018-09-13 核苷酸定序元件、芯片及定序分析方法

Country Status (6)

Country Link
US (1) US20210033563A1 (zh)
EP (1) EP3851843A4 (zh)
JP (1) JP7171901B2 (zh)
KR (1) KR20210089139A (zh)
CN (1) CN111356916B (zh)
WO (1) WO2020051824A1 (zh)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120021918A1 (en) * 2008-09-29 2012-01-26 Purdue Research Foundation DNA Sequencing and Amplification Systems Using Nanoscale Field Effect Sensor Arrays
CN103512940A (zh) * 2012-06-21 2014-01-15 Nxp股份有限公司 具有传感器的集成电路和制造方法
US20140209982A1 (en) * 2013-01-28 2014-07-31 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
CN104884948A (zh) * 2013-01-17 2015-09-02 株式会社日立高新技术 生物分子测量装置
CN105452856A (zh) * 2013-06-10 2016-03-30 生命科技公司 具有每孔多个传感器的化学传感器阵列
CN106244578A (zh) * 2015-06-09 2016-12-21 桑特里莱恩科技控股公司 用于对核酸进行测序的方法
WO2018026830A1 (en) * 2016-08-01 2018-02-08 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
CN107923927A (zh) * 2015-08-28 2018-04-17 夏普生命科学（欧洲）有限公司 液滴微流控设备及感测其中的测定结果的方法
WO2018153919A1 (en) * 2017-02-22 2018-08-30 Roche Diagnostics Gmbh Analyte detector for detecting at least one analyte in at least one fluid sample

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198754B2 (en) * 2001-08-31 2007-04-03 Kabushiki Kaisha Toshiba Biological material detection element, biological material detection method and apparatus, charged material moving apparatus
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20120261274A1 (en) * 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
WO2012152308A1 (en) * 2011-05-06 2012-11-15 X-Fab Semiconductor Foundries Ag Ion sensitive field effect transistor

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120021918A1 (en) * 2008-09-29 2012-01-26 Purdue Research Foundation DNA Sequencing and Amplification Systems Using Nanoscale Field Effect Sensor Arrays
CN103512940A (zh) * 2012-06-21 2014-01-15 Nxp股份有限公司 具有传感器的集成电路和制造方法
CN104884948A (zh) * 2013-01-17 2015-09-02 株式会社日立高新技术 生物分子测量装置
US20140209982A1 (en) * 2013-01-28 2014-07-31 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
CN105452856A (zh) * 2013-06-10 2016-03-30 生命科技公司 具有每孔多个传感器的化学传感器阵列
CN106244578A (zh) * 2015-06-09 2016-12-21 桑特里莱恩科技控股公司 用于对核酸进行测序的方法
CN107923927A (zh) * 2015-08-28 2018-04-17 夏普生命科学（欧洲）有限公司 液滴微流控设备及感测其中的测定结果的方法
WO2018026830A1 (en) * 2016-08-01 2018-02-08 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
WO2018153919A1 (en) * 2017-02-22 2018-08-30 Roche Diagnostics Gmbh Analyte detector for detecting at least one analyte in at least one fluid sample

Also Published As

Publication number Publication date
JP2022500055A (ja) 2022-01-04
CN111356916B (zh) 2022-05-24
EP3851843A1 (en) 2021-07-21
US20210033563A1 (en) 2021-02-04
EP3851843A4 (en) 2022-04-27
JP7171901B2 (ja) 2022-11-15
KR20210089139A (ko) 2021-07-15
WO2020051824A1 (zh) 2020-03-19

Similar Documents

Publication Publication Date Title
JP3903183B2 (ja) 遺伝子検出電界効果デバイスおよびこれを用いた遺伝子多型解析方法
US9322798B2 (en) Diamond electrode nanogap transducers
CN100447563C (zh) 利用生物分子检测元件分析核酸的方法
US10429342B2 (en) Chemically-sensitive field effect transistor
US20180180567A1 (en) Microwell electrode and method for analysis of a chemical substance
CN107407656B (zh) 使用大规模 fet 阵列测量分析物的方法和装置
US8674086B2 (en) Nucleotides and oligonucleotides for nucleic acid sequencing
US20080286767A1 (en) Method of Analyzing Dna Sequence Using Field-Effect Device, and Base Sequence Analyzer
US20190367977A1 (en) High speed molecular sensing with nanopores
WO2006041224A1 (en) Fet-based nucleic acid detecting sensor
CN110873750A (zh) 具有每孔多个传感器的化学传感器阵列
US20190064141A1 (en) Exporting measurements of nanopore arrays
CN111356916B (zh) 核苷酸定序元件、芯片及定序分析方法
US11686701B2 (en) Nanopore device and methods of detecting and classifying charged particles using same
US20190300932A1 (en) Nano-deterministic lateral displacement array for next-generation sequencing sample preparation and high-sensitivity dna detection
JP6367295B2 (ja) ダイヤモンド電極ナノギャップトランスデューサ
CN204097484U (zh) 一种砂梨基因检测芯片

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant