Disclosure of Invention
The invention aims to provide a production process of bovine skin collagen peptide, which is a production method of bovine skin collagen peptide with high yield, good sensory effect and strong antioxidant activity.
The technical purpose of the invention is realized by the following technical scheme:
A production process of cow hide collagen peptide comprises the following steps:
s1 cleaning: cleaning 100 parts of cow leather by using clear water, soaking the cleaned cow leather in 2000 parts of water by weight, adding an alkaline regulator to regulate the pH value to be strong alkaline, adding 0.5-2 parts of a bactericide, and soaking for 48-240 hours;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 to neutral pH, cutting and grinding the cleaned cow leather into cow leather slurry, adding water with the weight part of 4-6 times that of the cow leather slurry into the cow leather slurry, and preserving heat for a certain time to obtain modified cow leather protein slurry;
s3 enzymolysis: adding 2-5 parts by mass of alkaline protease into the denatured cow leather protein slurry obtained in the step S2, keeping a certain temperature and pH, reacting for a period of time, and performing enzymolysis with ultrasonic waves in an auxiliary manner in the enzymolysis process to finally obtain an enzymolysis solution;
s4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, and taking supernate;
s5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 at constant temperature by adopting a ceramic membrane to obtain a product dilute liquid; concentrating the dilute liquid product to solid content over 20% by using membrane equipment, and removing microorganisms from the concentrated liquid by using a filter element to obtain concentrated liquid;
S7 finished product: and (5) spray-drying the concentrated solution obtained in the step S6 to obtain a solid, and inspecting and packaging the solid product to obtain a finished product.
By adopting the technical scheme, the production conditions of bovine hide collagen decomposition are optimized, particularly bactericide and denaturation treatment are carried out, strong basicity and bactericide are used for one-time denaturation in the S1 cleaning process, so that the removal of unnecessary impurity components is facilitated, the toughness of the bovine hide is adjusted, the bovine hide is conveniently milled into slurry in the follow-up process, and the process can be quickly adjusted by carrying out small-scale pre-experiments according to different batches of bovine hides in different production places; in the denaturation process of S2, secondary denaturation is carried out, and the cow leather slurry is treated at a proper temperature for a period of time in advance for denaturation, so that the subsequent enzymolysis efficiency is greatly improved, and the enzymolysis requirement is reduced; thus milder conditions may be used in the S3 enzymatic step.
The invention is further provided with: the alkaline regulator in the step S1 comprises NaOH and Na2CO3、NaHCO3、K2CO3、KHCO3、CaCO3And one or more of CaO, and the pH value is adjusted to 10-14.
By adopting the technical scheme, the alkaline regulators have low cost on one hand, and even a small amount of ion residues do no harm to human bodies on the other hand.
The invention is further configured as follows: the bactericide in the step S1 is H2O2One or more of lysozyme, stable chlorine dioxide and sodium hypochlorite.
Through adopting above-mentioned technical scheme, the majority is strong oxidant, uses under the not good condition of cow hide quality, supplementary regulation cow hide toughness when disinfecting, makes things convenient for the homogenate, when the cow hide quality is better, can adopt comparatively mild lysozyme, furthest's protection collagen.
The invention is further configured as follows: and the heat preservation temperature in the S2 denaturation is set within 60-80 ℃, and the heat preservation time is set within 4-6 h.
By adopting the technical scheme, the denaturation treatment is performed in advance, and the subsequent enzymolysis is facilitated.
The invention is further provided with: in the step S3, the reaction temperature is set to be 50-65 ℃, the reaction pH is set to be 7.5-9.5, the reaction time is set to be 5-6h, and the power of the ultrasonic wave is 200-900 kW.
By adopting the technical scheme, the enzymolysis process is mild, ultrasonic enzymolysis is adopted in an auxiliary way, the enzymolysis efficiency is improved, and meanwhile, the content of ultra-small molecules is reduced by inhibiting the enzyme activity through the environment, so that the content of amino terminal residues is reduced, and the uncomfortable taste of small molecular peptides generated by decomposing bovine collagen is reduced.
The invention is further configured as follows: in the step S4 of centrifugation, a tubular centrifuge is adopted, the centrifugation speed is more than 10000r/min, and the temperature is kept between 40 and 60 ℃ during centrifugation.
The invention is further configured as follows: in the step S6, in the impurity removal, the aperture of the used ceramic membrane is set at 8000-14000Dal, and the feed liquid and the ambient temperature are controlled to be 60-80 ℃ during the filtration; the aperture of the membrane equipment is set at 500-600 Dal; the aperture of the filter element is set to be 0.22 μm.
By adopting the technical scheme, the peptide with ultra-small molecular weight can be removed, the average molecular weight is further improved, and the unpleasant odor is reduced; meanwhile, various impurities such as pigment and the like are removed, and the product quality is improved.
The invention is further provided with: the air inlet temperature of the spray drying is set to be 150-180 ℃, and the air outlet temperature is set to be 70-80 ℃.
The invention has the beneficial effects that:
1. the application provides a denaturation technology for handling cow hide in advance, reduces its difference in quality, has reduced follow-up cow hide processing degree of difficulty for even the cow hide raw materials of many sources also can be the processing of high efficiency low cost become the stable bovine collagen peptide product of quality homogeneity.
2. The method adopts the combined action of an ultrasonic process, a denaturation process and an enzymolysis process, and the denaturation process actively destroys the connection structure between proteins, so that the bovine skin collagen is more dispersed; ultrasonic waves destroy the space structure of collagen, expose more enzymolysis sites and increase the enzymolysis efficiency; under the combined action of the two, the reaction condition of enzymolysis can be milder, the energy consumption is reduced, the environment deviates from the optimal environment of protease, the enzyme digestion efficiency of the protease is inhibited, the product is more inclined to the peptide with larger molecular weight, the generation amount of the ultra-small molecular peptide is greatly reduced, and the ultra-small molecular peptide is the root cause of generating unpleasant temperature.
3. According to the method, the processes of membrane filtration and filter element filtration are adopted for impurity removal and sterilization, so that on one hand, the influence of high temperature on the activity of the small molecular peptide is avoided, and the small molecular peptide in the product has higher biological oxidation resistance activity; on the other hand, the Maillard reaction of the product is avoided without adopting high-temperature sterilization liquid, the generation of pigment is reduced, and the requirement on color removal is reduced, so that the requirement on color removal of the product can be met by membrane filtration, and the loss of protein peptide caused by adopting substances such as activated carbon is also avoided.
Detailed Description
The technical solutions in the examples will be clearly and completely described below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments.
Example 1
A production process of bovine skin collagen peptide comprises the following steps:
s1 cleaning: cleaning 100 parts of cow leather with clear water, soaking the cleaned cow leather in 1400 parts of water, adding NaOH to adjust the pH value to 13, and adding 1.35 parts of H2O2Soaking for 78 h;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 until the pH value is neutral, then cutting and grinding the clean cow leather to prepare cow leather slurry, adding 5 parts by weight of water into the cow leather slurry, setting the heat preservation temperature in denaturation to 72 ℃ and the heat preservation time to 5.5 hours to obtain denatured cow leather protein slurry;
s3 enzymolysis: adding 3.75 parts of alkaline protease into the denatured cow leather protein slurry obtained in the step S2, keeping the temperature at 57 ℃, keeping the pH at 8.2, reacting for 5.5 hours, and performing enzymolysis with ultrasonic waves in an auxiliary manner in the enzymolysis process, wherein the power of the ultrasonic waves is 700kW, so as to finally obtain an enzymolysis liquid;
s4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, centrifuging at 20000r/min in a tubular centrifuge at 55 deg.C, and collecting supernatant;
s5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 at constant temperature by adopting a ceramic membrane with the aperture of 10000Dal, and controlling the temperature of the liquid and the environment to be 72 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 550Dal until the solid content is more than 20%, and removing microorganisms from the concentrated liquid by a filter element with the aperture of 0.22 mu m to obtain concentrated liquid;
S7 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 170 ℃ and the air outlet temperature to be 75 ℃, and inspecting and packaging the solid product to obtain a finished product.
Example 2
A production process of bovine skin collagen peptide comprises the following steps:
s1 cleaning: cleaning 100 parts of cow leather with clear water, immersing the cleaned cow leather in 1000 parts of water, adding NaOH and Na2CO3Adjusting the pH value to 10, adding 2 parts of stable chlorine dioxide, and soaking for 50 h;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 until the pH value is neutral, then cutting and grinding the clean cow leather to prepare cow leather slurry, adding 4 parts by weight of water into the cow leather slurry, setting the heat preservation temperature in denaturation to be 60-80 ℃, and setting the heat preservation time to be 6h to obtain denatured cow leather protein slurry;
s3 enzymolysis: adding 2 parts of alkaline protease into the denatured cow leather protein slurry obtained in the step S2, keeping the temperature at 65 ℃, keeping the pH at 7.5, reacting for 6 hours, and performing enzymolysis with ultrasonic waves assisted in the enzymolysis process, wherein the power of the ultrasonic waves is 300kW, so as to finally obtain an enzymolysis liquid;
s4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, centrifuging by a tubular centrifuge at the rotating speed of 22000r/min, keeping the temperature at 40 ℃ during centrifugation, and taking supernatant;
S5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 at constant temperature by adopting a ceramic membrane with the aperture of 14000Dal, and controlling the temperature of the liquid and the environment to be 60 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 600Dal until the solid content is more than 20%, and removing microorganisms from the concentrated liquid by a filter element with the aperture of 0.22 mu m to obtain concentrated liquid;
s7 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 150 ℃ and the air outlet temperature to be 80 ℃, and inspecting and packaging the solid product to obtain a finished product.
Example 3
A production process of cow hide collagen peptide comprises the following steps:
s1 cleaning: cleaning 100 parts of cowhide by using clear water, soaking the cleaned cowhide in 2000 parts of water, adding NaOH and CaO, adjusting the pH value to 14, adding 0.5 part of sodium hypochlorite, and soaking for 200 hours;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 until the pH value is neutral, then cutting and grinding the clean cow leather to prepare cow leather slurry, adding 6 parts by weight of water into the cow leather slurry, setting the heat preservation temperature in denaturation to be 80 ℃, and setting the heat preservation time to be 4 hours to obtain denatured cow leather protein slurry;
S3 enzymolysis: adding 5 parts of alkaline protease into the denatured cow leather protein slurry obtained in the denaturation step S2, keeping the temperature at 50 ℃, keeping the pH at 9.5, reacting for 5 hours, and carrying out enzymolysis with ultrasonic waves in an auxiliary manner in the enzymolysis process, wherein the power of the ultrasonic waves is 850kW, so as to finally obtain an enzymolysis solution;
s4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, performing tubular centrifuge at a rotation speed of 12000r/min, keeping the temperature at 60 ℃ during centrifugation, and taking supernate;
s5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 at constant temperature by adopting a ceramic membrane with the aperture of 8000Dal, and controlling the temperature of the liquid and the environment to be 80 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 500Dal until the solid content is more than 20%, and then removing microorganisms from the concentrated liquid by a filter element with the aperture of 0.22 mu m to obtain concentrated liquid;
s7 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 180 ℃ and the air outlet temperature to be 70 ℃, and inspecting and packaging the solid product to obtain a finished product.
Comparative example 1
Comparative example 1 is different from example 1 in that only the denaturation of S2 is not performed, and the specific scheme is as follows:
s1 cleaning: cleaning 100 parts of cow leather with clear water, soaking the cleaned cow leather in 2000 parts of water, adding NaOH to adjust the pH value to 13, and adding 1.35 parts of H2O2Soaking for 78h, and cleaning the cowhide to be neutral;
s2 enzymolysis: adding 3.75 parts of alkaline protease into the clean cowhide obtained in the step S1, keeping the temperature at 57 ℃, keeping the pH at 8.2, reacting for 5.5h, and performing enzymolysis by using ultrasonic waves with the ultrasonic wave power of 700kW in the enzymolysis process to finally obtain an enzymolysis liquid;
s3 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, centrifuging at 20000r/min in a tubular centrifuge at 55 deg.C, and collecting supernatant;
s4 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s5 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 at constant temperature by adopting a ceramic membrane with the aperture of 10000Dal, and controlling the temperature of the liquid and the environment to be 72 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 550Dal until the solid content is more than 20%, and removing microorganisms from the concentrated liquid by a filter element with the aperture of 0.22 mu m to obtain concentrated liquid;
S6 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 170 ℃ and the air outlet temperature to be 75 ℃, and inspecting and packaging the solid product to obtain a finished product.
Comparative example 2
Compared with example 1, the difference between comparative example 1 and example 1 is that no ultrasonic wave is used only in the enzymolysis of S3, and the specific scheme is as follows:
s1 cleaning: cleaning 100 parts of cow leather with clear water, soaking the cleaned cow leather in 1400 parts of water, adding NaOH to adjust the pH value to 13, and adding 1.35 parts of H2O2Soaking for 78 h;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 until the pH value is neutral, then cutting and grinding the clean cow leather to prepare cow leather slurry, adding 5 parts by weight of water into the cow leather slurry, setting the heat preservation temperature in denaturation to 72 ℃ and the heat preservation time to 5.5 hours to obtain denatured cow leather protein slurry;
s3 enzymolysis: to the denatured bovine hide protein slurry obtained in the denaturation in step S2, 3.75 parts of alkaline protease was added, the temperature was maintained at 57 ℃, the pH was maintained at 8.2, and the reaction was carried out for 5.5 hours.
S4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, centrifuging at 20000r/min in a tubular centrifuge at 55 deg.C, and collecting supernatant;
S5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 by adopting a ceramic membrane with the aperture of 10000Dal at constant temperature, and controlling the temperature of the liquid and the environment to be 72 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 550Dal until the solid content is more than 20%, and then removing microorganisms from the concentrated liquid by a filter element with the aperture of 0.22 μm to obtain concentrated liquid;
s7 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 170 ℃ and the air outlet temperature to be 75 ℃, and inspecting and packaging the solid product to obtain a finished product.
Comparative example 3
Compared with the example 1, the difference of the comparative example 1 is that the filter element sterilization is not used when the S6 is subjected to impurity removal, and the ultrahigh temperature sterilization with the temperature of 180 ℃ lasting for 30S is adopted, and the specific scheme is as follows:
s1 cleaning: cleaning 100 parts of cow leather with clear water, soaking the cleaned cow leather in 1400 parts of water, adding NaOH to adjust the pH value to 13, and adding 1.35 parts of H2O2Soaking for 78 h;
s2 denaturation: cleaning the clean cow leather obtained in the step S1 until the pH value is neutral, then cutting and grinding the clean cow leather to prepare cow leather slurry, adding 5 parts by weight of water into the cow leather slurry, setting the heat preservation temperature in denaturation to 72 ℃ and the heat preservation time to 5.5 hours to obtain denatured cow leather protein slurry;
S3 enzymolysis: adding 3.75 parts of alkaline protease into the denatured cow leather protein slurry obtained in the denaturation step S2, keeping the temperature at 57 ℃, keeping the pH at 8.2, reacting for 5.5 hours, and carrying out enzymolysis with ultrasonic waves in an auxiliary manner in the enzymolysis process, wherein the power of the ultrasonic waves is 700kW, so as to finally obtain an enzymolysis solution;
s4 centrifugation: centrifuging the enzymolysis liquid obtained in the step S3, centrifuging at 20000r/min in a tubular centrifuge at 55 ℃, and taking supernatant;
s5 enzyme deactivation: heating the supernatant obtained in the centrifugation in the step S4 to 95 ℃, and inactivating the enzyme for 10min to obtain an enzyme inactivation solution;
s6 impurity removal: filtering the enzyme deactivation liquid obtained in the step S5 by adopting a ceramic membrane with the aperture of 10000Dal at constant temperature, and controlling the temperature of the liquid and the environment to be 72 ℃ during filtering to obtain a dilute liquid product; concentrating the dilute feed liquid product by membrane equipment with the aperture of 550Dal until the solid content is more than 20%, and then sterilizing at the ultra-high temperature of 180 ℃ for 30s to obtain concentrated solution;
s7 finished product: and (5) carrying out spray drying on the concentrated solution obtained in the step (S6) to obtain a solid, setting the air inlet temperature of a spray dryer to be 170 ℃ and the air outlet temperature to be 75 ℃, and inspecting and packaging the solid product to obtain a finished product.
After all production is finished, the final finished product is taken, the oxidation resistance, the product molecular weight distribution, the product yield and the product sensory contrast are detected, and the detection method and the result are as follows:
1. oxidation resistance assay
The currently generally used in-vitro antioxidant activity index generally adopts a spectrophotometry method, and a spectrophotometer is used for measuring the change of the content of various color components. The spectrophotometry method is simple and convenient to operate, and does not need special detection equipment.
DPPH shows more stable radical scavenging ability in purple color in methanol or ethanol solution, and has stronger ultraviolet absorption peak at 517 nm. When an antioxidant is present in DPPH.solution, lone pair electrons in DPPH.are paired, the color of the solution changes from purple to yellow, the absorption value at 517nm decreases,
first, 1.5mL of sample was added to 1.5mL of 0.1mmol/L DPPH (95% ethanol), mixed well and incubated at 25 ℃ for 30 min. The absorbance was measured at 517 nm. The Vc solution was used as a control. DPPH-scavenging capacity W (%) was calculated as follows:
wherein A0 is the absorbance value for 1.5mL of distilled water and 1.5mL of 95% ethanol containing 0.1mmol/L DPPH, A1 is the absorbance value for 1.5mL of hydrolysate containing 0.1mmol/L DPPH, and A2 is the absorbance value for 1.5mL of hydrolysate and 1.5mL of 95% ethanol.
The detection results are shown in fig. 1:
as shown in the figure, the DPPH removing capacity of the collagen increases greatly along with the increase of the concentration of the bovine collagen, and finally the DPPH removing capacity approaches 80% at 126.4mg/mL, which indicates that the bovine collagen has stronger antioxidant capacity, and examples 1, 2 and 3 all approach to the upper removing limit at about 63 mg/mL.
In contrast, comparative example 1 has no denaturation treatment and has fewer enzyme cutting sites, so that the active components are fewer and the antioxidant capacity is lower; in the comparative example 2, the enzymolysis is not assisted by ultrasonic waves, and the tertiary quaternary structure of the protein still exists, so that enzyme cutting sites are fewer, and the method is similar to the comparative example 1; the use of ultra-high temperature flash sterilization in comparative example 3 resulted in a significant reduction in product activity.
Compared with similar products, the bovine collagen peptide DPPH produced by the process has stronger removing capability, higher activity and better oxidation resistance.
2. Determination of product molecular weight distribution and product yield
The obtained product was sampled and analyzed by chromatography, the analysis method refers to appendix A in GB/T22729-2008, the ratio of the protein hydrolysate with the relative molecular mass less than 1000u (high performance gel filtration chromatography) was obtained, the molecular weight range and the number average molecular weight were obtained, the yield was calculated from the yield and the input, and the final results are shown in Table 1
TABLE 1 Table of the results of the molecular weight and yield measurements
The method can easily see that the product yield is improved to a certain extent by simultaneously using ultrasonic-assisted enzymolysis and denaturation processes, and the products with ultra-small molecular weight in the blended production process are few, so that the yield is greatly improved; although comparative example 3 only changes the subsequent treatment into ultrahigh-temperature instantaneous sterilization, and the extraction process is not changed too much, the yield is about the same as that of examples 1, 2 and 3, but the yield is always slightly lower than that of other methods according to the application, and comparative example 3 also has a serious browning phenomenon in the actual production, and the results of data in a table and the actual production phenomenon show that the extracting solution contains reducing sugar, comparative example 3 has serious browning after being subjected to ultrahigh-temperature sterilization, and excessive peptides and reducing sugar undergo Maillard reaction.
3. Product sensory comparison
Taking the off-flavor of clear water as 0 and the off-flavor of a 10% solution of commercially available bovine collagen peptide as 10, 10 professional evaluators were found for off-flavor evaluation, and the results are shown in table 2, after removing one highest score and one lowest score, the average value was taken:
TABLE 2 sense organ examination table for products
|
Example 1
|
Example 2
|
Example 3
|
Comparative example 1
|
Comparative example 2
|
Comparative example 3
|
Evaluator 1
|
7
|
7
|
6
|
9
|
9
|
9
|
Evaluator 2
|
6
|
7
|
8
|
10
|
9
|
7
|
Evaluator 3
|
7
|
7
|
7
|
9
|
9
|
8
|
Evaluator 4
|
6
|
7
|
7
|
9
|
9
|
8
|
Evaluator 5
|
6
|
7
|
8
|
9
|
8
|
7
|
Evaluator 6
|
8
|
7
|
7
|
9
|
10
|
8
|
Evaluator 7
|
7
|
7
|
7
|
9
|
10
|
8
|
Evaluator 8
|
6
|
7
|
8
|
9
|
9
|
8
|
Evaluator 9
|
7
|
7
|
7
|
10
|
8
|
8
|
Evaluator 10
|
7
|
7
|
7
|
8
|
9
|
8
|
Mean value
|
6.6
|
7.0
|
7.3
|
9.1
|
9.0
|
7.9 |
It can be seen from the table that the odor is more obvious in comparative example 1 and comparative example 2 without adjusting the production conditions, because the production conditions are not adjusted, the enzyme cutting sites are less, and the small molecular peptides extracted by the prior art scheme are more biased, while the scorched odor is more obvious in comparative example 3, and the product taste is lower. The new process adopts a denaturation process and ultrasonic waves to expose more enzyme cutting sites and simultaneously improve the enzymolysis speed, and the reaction environment inhibits the enzyme activity and reduces excessive cutting, so that the obtained product has proper length distribution, the ultra-small molecular peptides generating odor and peculiar smell are greatly reduced, the uncomfortable taste of the product is reduced, and the product quality is improved.
In conclusion, after the novel method provided by the application is adopted for production, the extracted bovine collagen peptide has high yield, good sense, high oxidation resistance activity, extremely low browning rate and high product quality.
All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.