CN111330088A - Preparation method of nanofiber dermis scaffold - Google Patents
Preparation method of nanofiber dermis scaffold Download PDFInfo
- Publication number
- CN111330088A CN111330088A CN202010137502.4A CN202010137502A CN111330088A CN 111330088 A CN111330088 A CN 111330088A CN 202010137502 A CN202010137502 A CN 202010137502A CN 111330088 A CN111330088 A CN 111330088A
- Authority
- CN
- China
- Prior art keywords
- nanofiber
- membrane
- preparing
- dermal scaffold
- dermal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method of a nanofiber dermal scaffold, which comprises the following steps: (1) dissolving 20-25 wt% of human recombinant collagen and 0-5 wt% of auxiliary materials in a solvent to prepare spinning solution; (2) preparing a nanofiber membrane by electrostatic spinning; (3) crosslinking the nanofiber membrane by a crosslinking agent; (4) and loading the crosslinked nanofiber membrane on a silica gel membrane to obtain the nanofiber dermal scaffold. The scaffold prepared by the invention has good fiber appearance and high mechanical strength, meets the requirements of medical dressings, can be well attached to injured tissue parts, effectively helps dermal tissue regeneration, accelerates wound healing, maintains wound surface moistening and reduces the risk of scar formation.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method of a nanofiber dermal scaffold.
Background
Collagen (Collagen), also known as Collagen, is a helical fibrous protein formed by three peptide chains. The collagen is important protein of animal connective tissue, the connective tissue contains 60-70% of water, and the collagen accounts for about 20-30%, and the connective tissue has certain structural and mechanical properties, such as tensile strength, tensile force, elastic force and the like, so as to achieve the functions of supporting and protecting due to the high content of the collagen.
The collagen monomer is long cylindrical protein, the length of the collagen monomer is about 280nm, and the diameter of the collagen monomer is 1.4-1.5 nm. It is made up by winding 3 polypeptide chains in supercoiled form. Collagen is composed of a group of proteins that are slightly different in structure, and 27 different types of collagen have been found, and classified into type I collagen, type II collagen, type IM collagen, and the like in the order of finding, and the most common type is type I collagen.
In order to improve the strength and modulus of collagen fibers, many researchers modify collagen or add other materials to increase the mechanical properties of collagen fibers, such as adding polyethylene oxide into an acidic solution of collagen, and preparing blend nanofibers by electrostatic spinning, wherein the fiber diameter is between 100 nm and 150 nm. Due to the interaction between PEO and collagen, the fibers have better mechanical properties. However, the introduction of other polymer materials has many limitations in application, and the safety of the collagen membrane is greatly reduced.
Disclosure of Invention
The invention mainly provides a preparation method of a nanofiber dermal scaffold, which has good fiber morphology, meets the requirements of medical dressings, can be well attached to a damaged tissue part, effectively helps the regeneration of dermal tissues and accelerates the healing of wounds. The technical scheme is as follows:
a preparation method of a nanofiber dermal scaffold comprises the following steps:
(1) dissolving 20-25 wt% of human recombinant collagen and 0-5 wt% of auxiliary materials in a solvent to prepare spinning solution;
(2) preparing a nanofiber membrane by electrostatic spinning;
(3) crosslinking the nanofiber membrane by a crosslinking agent;
(4) and loading the crosslinked nanofiber membrane on a silica gel membrane to obtain the nanofiber dermal scaffold.
Preferably, the auxiliary materials in the step (1) are selected from one or more of sodium hyaluronate, sodium polyglutamate and chondroitin sulfate.
Preferably, the solvent in step (1) is one or more selected from hexafluoroisopropanol, purified water and ethanol.
Preferably, step (3) is carried out for steam crosslinking for 1-6h or thermal crosslinking for 12-72h by using a glutaraldehyde crosslinking agent.
Preferably, in the step (4), the silica gel is coated on a 50-200 μm silica gel membrane, and the gram weight of the coated silica gel membrane is 50-300g/m2。
Preferably, the nanofiber dermal scaffold has a water vapor transmission rate of 100-2·24h)。
By adopting the scheme, the invention has the following advantages:
the nanofiber dermal scaffold prepared by the invention has good fiber morphology, can be well attached to a damaged tissue part, effectively helps dermal tissue to regenerate, accelerates wound healing, prevents bacteria from invading a wound surface, prevents wound infection, and has a certain inhibiting effect on scar formation.
Detailed Description
The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental reagents and materials involved are conventional biochemical reagents and materials unless otherwise specified.
Example 1
The preparation method of the nanofiber dermal scaffold of the embodiment is as follows:
(1) dissolving 22 wt% of human recombinant collagen and 2 wt% of sodium hyaluronate in hexafluoroisopropanol to prepare spinning solution;
(2) preparing a nanofiber membrane by electrostatic spinning;
(3) carrying out steam crosslinking on the nanofiber membrane for 6h through glutaraldehyde;
(4) loading the crosslinked nanofiber membrane on a 200-micron silica gel membrane, wherein the gram weight of the coated gel is 200g/m2Obtaining the nano-fiber dermal scaffold with the water vapor transmission rate of 540 g/(m)2·24h)。
Example 2
The preparation method of the nanofiber dermal scaffold of the embodiment is as follows:
(1) dissolving 20 wt% of human recombinant collagen, 2 wt% of sodium hyaluronate and 2 wt% of chondroitin sulfate in ethanol to prepare spinning solution;
(2) preparing a nanofiber membrane by electrostatic spinning;
(3) thermally crosslinking the nanofiber membrane by glutaraldehyde;
(4) loading the crosslinked nanofiber membrane on a 50-micron silica gel membrane, wherein the gram weight of the coated gel is 100g/m2Obtaining the nano-fiber dermal scaffold with the water vapor transmission rate of 1000 g/(m)2·24h)。
Example 3
The preparation method of the nanofiber dermal scaffold of the embodiment is as follows:
(1) dissolving 25 wt% of human recombinant collagen and 3 wt% of sodium polyglutamate in a mixed solution of purified water and ethanol to prepare a spinning solution;
(2) preparing a nanofiber membrane by electrostatic spinning;
(3) thermally crosslinking the nanofiber membrane for 72h through glutaraldehyde;
(4) loading the crosslinked nanofiber membrane on a 200-micron silica gel membrane, wherein the gram weight of the coated gel is 300g/m2Obtaining the nano-fiber dermal scaffold with the water vapor transmission rate of 820 g/(m)2·24h)。
Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present invention.
Claims (6)
1. A preparation method of a nanofiber dermal scaffold is characterized by comprising the following steps: the method comprises the following steps:
(1) dissolving 20-25 wt% of human recombinant collagen and 0-5 wt% of auxiliary materials in a solvent to prepare spinning solution;
(2) preparing a nanofiber membrane by electrostatic spinning;
(3) crosslinking the nanofiber membrane by a crosslinking agent;
(4) and loading the crosslinked nanofiber membrane on a silica gel membrane to obtain the nanofiber dermal scaffold.
2. The method for preparing a nanofiber dermal scaffold according to claim 1, characterized in that: the auxiliary materials in the step (1) are selected from one or more of sodium hyaluronate, sodium polyglutamate and chondroitin sulfate.
3. The method for preparing a nanofiber dermal scaffold according to claim 1, characterized in that: in the step (1), the solvent is one or more selected from hexafluoroisopropanol, purified water and ethanol.
4. The method for preparing a nanofiber dermal scaffold according to claim 1, characterized in that: in the step (3), a glutaraldehyde crosslinking agent is used for steam crosslinking for 1-6h or thermal crosslinking for 12-72 h.
5. The method for preparing a nanofiber dermal scaffold according to claim 1, characterized in that: in the step (4), silica gel is coated on a 50-200 mu m silica gel membrane, and the gram weight of the coated silica gel membrane is 50-300g/m2。
6. The method for preparing a nanofiber dermal scaffold according to claim 1, characterized in that: the water vapor transmission rate of the nano-fiber dermal scaffold is 100-1000 g/(m)2·24h)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010137502.4A CN111330088A (en) | 2020-03-03 | 2020-03-03 | Preparation method of nanofiber dermis scaffold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010137502.4A CN111330088A (en) | 2020-03-03 | 2020-03-03 | Preparation method of nanofiber dermis scaffold |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111330088A true CN111330088A (en) | 2020-06-26 |
Family
ID=71174019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010137502.4A Pending CN111330088A (en) | 2020-03-03 | 2020-03-03 | Preparation method of nanofiber dermis scaffold |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111330088A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111821515A (en) * | 2020-08-10 | 2020-10-27 | 青海创铭医疗器械有限公司 | Chitosan-human-derived recombinant collagen electrostatic spinning nanofiber scaffold and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104047115A (en) * | 2014-07-04 | 2014-09-17 | 东华大学 | Electrostatic spinning preparation method of collagen nanofiber membrane capable of releasing vitamin E |
| CN107050521A (en) * | 2017-04-27 | 2017-08-18 | 天新福（北京）医疗器材股份有限公司 | A kind of double-layer collagen dermis scaffold and preparation method thereof |
| WO2018124887A1 (en) * | 2016-12-28 | 2018-07-05 | Auckland Uniservices Ltd | Electrospun matrix and method |
| CN110025830A (en) * | 2019-04-29 | 2019-07-19 | 振德医疗用品股份有限公司 | A kind of sustained release antibacterial type artificial dermis model and its construction method |
-
2020
- 2020-03-03 CN CN202010137502.4A patent/CN111330088A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104047115A (en) * | 2014-07-04 | 2014-09-17 | 东华大学 | Electrostatic spinning preparation method of collagen nanofiber membrane capable of releasing vitamin E |
| WO2018124887A1 (en) * | 2016-12-28 | 2018-07-05 | Auckland Uniservices Ltd | Electrospun matrix and method |
| CN107050521A (en) * | 2017-04-27 | 2017-08-18 | 天新福（北京）医疗器材股份有限公司 | A kind of double-layer collagen dermis scaffold and preparation method thereof |
| CN110025830A (en) * | 2019-04-29 | 2019-07-19 | 振德医疗用品股份有限公司 | A kind of sustained release antibacterial type artificial dermis model and its construction method |
Non-Patent Citations (1)
| Title |
|---|
| 盛嘉隽: ""重组人胶原纳米纤维支架的制备及其生物相容性检测"", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111821515A (en) * | 2020-08-10 | 2020-10-27 | 青海创铭医疗器械有限公司 | Chitosan-human-derived recombinant collagen electrostatic spinning nanofiber scaffold and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cui et al. | Electrospun chitosan nanofibers for wound healing application | |
| Liang et al. | Preparation of self‐regulating/anti‐adhesive hydrogels and their ability to promote healing in burn wounds | |
| JP3901215B2 (en) | fiber | |
| CN104096262B (en) | Collagen/chitosan/mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof | |
| CN111518289A (en) | Injectable self-healing hydrogel with adjustable mechanical properties and preparation method and application thereof | |
| WO2020116924A1 (en) | Pyrogallol group functionalized polysaccharide-based hemostatic tissue adhesive and composition | |
| CN102580166A (en) | Medical bionic transparent film implanting material, and preparation method and application of material | |
| CN110195294B (en) | Nanofiber membrane with double-load core/shell structure and preparation method thereof | |
| Sandri et al. | Electrospinning technologies in wound dressing applications | |
| CN112957521B (en) | Preparation method of alginate-silk fibroin composite hydrogel carrying artemisinin liposome | |
| CN104711702A (en) | Collagen aggregate compound medical fiber with antibacterial/bacteriostatic function | |
| CN109680350B (en) | Preparation method of collagen fiber | |
| CN111330088A (en) | Preparation method of nanofiber dermis scaffold | |
| CN111188194B (en) | Preparation method of silk fibroin/gelatin fiber reinforced chitosan composite hemostatic material | |
| CN110507842B (en) | Bacterial cellulose/hyaluronic acid/epsilon-polylysine functional dressing and preparation method thereof | |
| JP3790720B2 (en) | Composite fiber | |
| CN116688211A (en) | Regenerated cellulose non-woven fabric based on chitosan modification and preparation method and application thereof | |
| CN112891062B (en) | Multifunctional nanofiber-based composite hemostatic patch and preparation method thereof | |
| KR101182417B1 (en) | Artificial Nanofiber Amnion Membranes and Method of Making The Same | |
| JP4085113B2 (en) | Composite fiber | |
| CN113604958A (en) | Preparation method and application of anti-infection composite layer medical dressing | |
| CN110615913B (en) | Silk protein porous sponge and preparation method thereof | |
| CN114522275A (en) | Three-dimensional porous composite scaffold of oriented conductive fibers and preparation method thereof | |
| CN115154648B (en) | Preparation method of composite sponge hemostatic material based on modified chitosan fibers | |
| CN1176141C (en) | Prepn of dissolved liquid crystal of chitosan grafted polylactic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200626 |
|
| RJ01 | Rejection of invention patent application after publication |