CN111275774A - Method for acquiring image under microscope and electronic equipment - Google Patents
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- CN111275774A CN111275774A CN201911411109.3A CN201911411109A CN111275774A CN 111275774 A CN111275774 A CN 111275774A CN 201911411109 A CN201911411109 A CN 201911411109A CN 111275774 A CN111275774 A CN 111275774A
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Abstract
The invention relates to a method for acquiring an image under a microscope, which is suitable for an acquisition system of the image under the microscope, wherein the system comprises a microscope, an image acquisition device, a processor and a display, the image acquisition device is arranged on a third ocular lens of the microscope and is in communication connection with the processor, and the processor is connected with the display, and the acquisition method comprises the following steps: the method comprises the following steps that an image acquisition device acquires a pathological image of a pathological section under a microscope and transmits the pathological image to a processor; the processor receives a pathological image from the image acquisition device; the processor performs white balance processing on the pathological image and outputs an adjusted pathological image; a display receives and displays the adjusted pathology image from the processor. The embodiment of the application provides a method for acquiring an image under a microscope and electronic equipment, which are used for correcting errors caused by external light, so that the color display of a pathological image is normal, and a doctor can read the image conveniently.
Description
Technical Field
The invention relates to the field of medical image processing, in particular to a method for acquiring an image under a microscope and electronic equipment.
Background
White balance is literally understood to be the balance of white. White balance is an index describing the accuracy of white color generated by mixing three primary colors of red, green and blue in a display. White balance is a very important concept in the field of television photography, by which a series of problems of color reproduction and tone processing can be solved. White balance is generated along with the fact that electronic images reproduce true colors, and the white balance is earlier applied in the field of professional shooting. The white balance adjusting method is widely used in household electronic products (household video cameras and digital cameras), however, the white balance adjusting becomes simpler and easier due to the technical development, but many users do not know the working principle of the white balance well, and a lot of misareas exist in understanding. The method realizes that the camera image can accurately reflect the color condition of the shot object, and has the modes of manual white balance, automatic white balance and the like. Many people have problems when using digital cameras for photography: the image shot in the room of the fluorescent lamp appears green, the scenery shot under the indoor tungsten lamp light is yellowish, and the picture shot in the sunlight shadow is wonderfully bluish, which is caused by the white balance setting. For the medical field, with the wide application of digital reading, the image in a computer must be ensured to be clear without color difference, so that the cell morphology can be more accurately judged. The color temperatures of different microscopes are different, and images projected to a computer screen after being shot by a camera are displayed in different colors, wherein some images are yellow and some images are red, so that the diagnosis of doctors is influenced greatly.
Disclosure of Invention
The present application is proposed to solve the above-mentioned technical problems. The embodiment of the application provides a method for acquiring an image under a microscope and electronic equipment, which are used for correcting errors caused by external light, so that the color display of a pathological image is normal, and a doctor can read the image conveniently.
According to an aspect of the present application, there is provided a method for acquiring an image under a microscope, which is applicable to an acquiring system of an image under a microscope, the system includes a microscope, an image acquisition device, a processor and a display, the image acquisition device is installed on a third eyepiece of the microscope and is in communication connection with the processor, and the processor is connected with the display, the method includes: the method comprises the following steps that an image acquisition device acquires a pathological image of a pathological section under a microscope and transmits the pathological image to a processor; the processor receives a pathological image from the image acquisition device; the processor performs white balance processing on the pathological image and outputs an adjusted pathological image; a display receives and displays the adjusted pathology image from the processor.
Further, the processor performs white balance processing on the pathology image, and outputs an adjusted pathology image, including:
sorting the pixels of the pathological image, and selecting a white reference point; the RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter; r =255/RN；g=255/GN；b=255/BN(ii) a Adjusting the RGB value R of the pathological image according to the adjusting parametery:Gy:By=(Rx*r)(Gx*g)(BxB); outputting the adjusted pathology image.
Further, when the white reference point is selected, pixels of the pathological image under the current visual field of the microscope are sequenced, and after the abnormal bright point is removed, a proper pixel point is selected as the white reference point.
Further, the microscope is a microscope with a light source.
Further, the light source is located between the microscope stage and the camera of the image acquisition device.
Further, the processor performs white balance processing on the pathology image, and outputs an adjusted pathology image, including: taking the pathological section away from a microscope objective table, and directly irradiating a camera of the image acquisition device by a light source; selecting proper pixel points of the image under the microscope visual field as white reference points; the RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter; r =255/RN；g=255/GN；b=255/BN(ii) a Placing the pathological section back to a microscope objective table; adjusting the RGB value R of the pathological image according to the adjusting parametery:Gy:By=(Rx*r)(Gx*g)(BxB); outputting the adjusted pathology image.
According to yet another aspect of the present application, there is provided an electronic device comprising a processor; and a memory having stored therein computer program instructions which, when executed by the processor, cause the processor to perform the method of acquiring an image under a microscope.
According to yet another aspect of the present application, there is provided a computer readable medium having stored thereon computer program instructions which, when executed by a processor, cause the processor to perform the method of acquiring an image under a microscope.
Compared with the prior art, by adopting the method for acquiring the image under the microscope and the electronic equipment, the pathological image of the pathological section under the microscope can be acquired through the image acquisition device, and the pathological image is transmitted to the processor; the processor receives a pathological image from the image acquisition device; the processor performs white balance processing on the pathological image and outputs an adjusted pathological image; a display receives and displays the adjusted pathology image from the processor. Therefore, the pathological images obtained after adjustment can avoid the conditions of yellowing, reddening and the like, so that the color display of the cells is normal, the tone of the pathological images is restored to the primary color state, and the intensity of red, green and blue of the whole image is adjusted according to the image characteristics of the current pathological images so as to correct the error caused by external light, so that the cell display is normal and the doctor can read the images conveniently.
Drawings
The above and other objects, features and advantages of the present application will become more apparent by describing in more detail embodiments of the present application with reference to the attached drawings. The accompanying drawings are included to provide a further understanding of the embodiments of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the principles of the application. In the drawings, like reference numbers generally represent like parts or steps.
FIG. 1 is a flow chart of a method of acquiring an image under a microscope;
FIG. 2 is a processor white balance processing flow diagram;
FIG. 3 is a pathological image before white balance treatment;
FIG. 4 is a pathological image after white balance processing;
fig. 5 is a block diagram of an electronic device of the present application.
Detailed Description
Hereinafter, example embodiments of the present application will be described in detail with reference to the accompanying drawings. It should be understood that the described embodiments are only some embodiments of the present application and not all embodiments of the present application, and that the present application is not limited by the example embodiments described herein.
Summary of the application
The white balance is a calculation method for digital image color processing, which can accurately restore the colors of other objects by restoring the color of a white object (generating a pure white color effect). In order to realize digital reading of pathological sections, the image in a computer must be clear without color difference, so that the cell morphology of the pathological sections can be more accurately judged. When the image acquisition device (can be camera for microscope) puts the current field of vision of microscope into display (can be the computer screen) through the adapter, the cell colour on the pathological section through the dyeing can produce the change because of the projection light colour, and the photo of shooing under the occasion of different light can have different colour temperatures. The color temperatures of different microscopes are different, and the image display projected to a computer screen after being shot by the image acquisition device is also different, and some images are yellow and red, so that the diagnosis of doctors is influenced very much. For the section with higher cell staining requirement, misdiagnosis is easy to cause. For example, a picture taken in an environment illuminated by a tungsten lamp (light bulb) may be yellowish, and a CCD chip in a camera for a microscope has no way to automatically correct for changes in light as the human eye does.
In view of the above technical problems, the present application provides an image acquisition method with a function of adjusting white balance of an image under a microscope, so as to restore the color tone of a pathological image to a primary color state, and adjust the intensity of red, green and blue colors of the whole image according to the image characteristics of the current pathological image, so as to correct the error caused by external light, so that the cell display is normal, and the doctor can read the image conveniently.
Having described the general principles of the present application, various non-limiting embodiments of the present application will now be described with reference to the accompanying drawings.
Exemplary method
A method for acquiring an image under a microscope is suitable for an acquisition system of the image under the microscope. The system for acquiring the images under the microscope comprises the microscope, an image acquisition device, a processor and a display, wherein the image acquisition device can select a camera for the microscope, is arranged on a third ocular of the microscope and is used for acquiring pathological image information of the solid section under the microscope in real time. The processor is in communication connection with the image acquisition device, receives the pathological image from the image acquisition device, performs white balance processing on the pathological image information, and outputs the processed pathological image. A display is coupled to the processor for receiving and displaying the processed pathology image from the processor. The display and the microscope synchronously display the pathological image information of the same part. When the pathological section microscope is used, a doctor places a pathological section on the microscope glass carrying table, adjusts the microscope camera, reads the pathological section under the microscope, processes the image under the microscope by the processor and then displays the processed image on the display, and the doctor can see the analysis result of the same pathological image information from the display.
The method for acquiring an image under a microscope, as shown in fig. 1, specifically includes:
s10: the method comprises the following steps that an image acquisition device acquires a pathological image of a pathological section under a microscope and transmits the pathological image to a processor;
s20: the processor receives a pathological image from the image acquisition device;
s30: the processor performs white balance processing on the pathological image and outputs an adjusted pathological image;
s40: a display receives and displays the adjusted pathology image from the processor.
The method comprises two white balance processing methods, and the two processing methods can be used interactively or in a superposition mode according to the image quality of a display end and the light condition of a use place.
Method one, as shown in fig. 2:
s31: sequencing pixels of pathological images of the pathological section under the microscope acquired by an image acquisition device, and selecting a white reference point;
since there are gaps between cells, the color that emerges from the gaps between cells is considered to be the natural color, which is equal to the color of the light source directly illuminating the photosensitive element, and this color pigment is theoretically brightest. When the actual slice is converted into an image, bright spots, noise spots, abnormal bright spots and the like may exist on the image, so that the bright spots, the noise spots and the abnormal bright spots need to be removed when a white reference point is selected.
S32: the RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter;
r=255/RN；g=255/GN；b=255/BN；
s33: adjusting the RGB value R of the pathological image according to the adjusting parametery:Gy:By=(Rx*r)(Gx*g)(Bx*b)；
The pathological images obtained after adjustment avoid the conditions of yellow, red and the like, so that the cell color is displayed normally and is closer to the primary color state of pathological sections.
S34: outputting the adjusted pathology image.
The second method comprises the following steps:
when a white reference point is selected, the pathological section is taken away from a microscope objective table, light emitted by a light source between the microscope objective table and a camera of an image acquisition device is directly irradiated on the camera, an image transmitted to a processor is displayed as a white image, all pixels of the image are sorted according to brightness, and after bright spots, noise spots, abnormal bright spots and the like on the image are removed, a certain pixel with a proper brightness value is selected as a white reference color.
The RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter;
r=255/RN；g=255/GN；b=255/BN；
then the pathological section is put back to the objective table, the image acquisition device acquires the pathological image again, and the processor adjusts the RGB value R of the pathological image according to the adjustment parametery:Gy:By=(Rx*r)(Gx*g)(BxB), the obtained image is the primary color image after white balance correction;
the processor outputs the adjusted pathological images to be displayed on the display, and the pathological images before and after white balance are shown in fig. 3 and 4.
Exemplary electronic device
Next, an electronic apparatus of an embodiment of the present application is described with reference to fig. 5. The electronic device 10 may be an electronic device 10 integrated with the input means 13 or a stand-alone device separate from said input means, which stand-alone device may communicate with said input means for receiving the acquired input signals from the input means. The input device 13 may be an image acquisition device (which may be a microscope camera) installed on a microscope, and converts the solid section into a pathological image, i.e., an input signal, and transmits the pathological image to the processor 11 and/or the memory 12, or the pathological image may be pre-stored in the processor 11 and directly called. The electronic device 10 may be an electronic device 10 integrated with the output means 14 or a stand-alone device separate from said output means, which stand-alone device may communicate with said output means for outputting the image processed by the processor 11 on the output means 14. The output device 14 may be a display for displaying or presenting the pathology image.
As shown in fig. 5, the electronic device 10 includes one or more processors 11 and memory 12.
The processor 11 may be a Central Processing Unit (CPU) or other form of processing unit having data processing capabilities and/or instruction execution capabilities, and may control other components in the electronic device to perform desired functions.
Memory 12 may include one or more computer program products that may include various forms of computer-readable storage media, such as volatile memory and/or non-volatile memory. The volatile memory may include, for example, Random Access Memory (RAM), cache memory (cache), and/or the like. The non-volatile memory may include, for example, Read Only Memory (ROM), hard disk, flash memory, etc. One or more computer program instructions may be stored on the computer readable storage medium and executed by processor 11 to implement the acquiring of the under microscope image functionality of the embodiments of the present application described above.
Exemplary computer program product and computer-readable storage Medium
In addition to the above-described methods and apparatus, embodiments of the present application may also be a computer program product comprising computer program instructions that, when executed by a processor, cause the processor to perform the steps in the method of acquiring an image under a microscope according to embodiments of the present application described in the "exemplary methods" section of this specification, supra.
The computer program product may be written with program code for performing the operations of embodiments of the present application in any combination of one or more programming languages, including an object oriented programming language such as Java, C + + or the like and conventional procedural programming languages, such as the "C" programming language or similar programming languages. The program code may execute entirely on the user's computing device, partly on the user's device, as a stand-alone software package, partly on the user's computing device and partly on a remote computing device, or entirely on the remote computing device or server.
Furthermore, embodiments of the present application may also be a computer-readable storage medium having stored thereon computer program instructions that, when executed by a processor, cause the processor to perform the steps in the method of acquiring an image under a microscope according to embodiments of the present application described in the "exemplary methods" section above in this specification.
The computer-readable storage medium may take any combination of one or more readable media. The readable medium may be a readable signal medium or a readable storage medium. A readable storage medium may include, for example, but not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or a combination of any of the foregoing. More specific examples (a non-exhaustive list) of the readable storage medium include: an electrical connection having one or more wires, a portable disk, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing.
The foregoing describes the general principles of the present application in conjunction with specific embodiments, however, it is noted that the advantages, effects, etc. mentioned in the present application are merely examples and are not limiting, and they should not be considered essential to the various embodiments of the present application. Furthermore, the foregoing disclosure of specific details is for the purpose of illustration and description and is not intended to be limiting, since the foregoing disclosure is not intended to be exhaustive or to limit the disclosure to the precise details disclosed.
The block diagrams of devices, apparatuses, systems referred to in this application are only given as illustrative examples and are not intended to require or imply that the connections, arrangements, configurations, etc. must be made in the manner shown in the block diagrams. These devices, apparatuses, devices, systems may be connected, arranged, configured in any manner, as will be appreciated by those skilled in the art. Words such as "including," "comprising," "having," and the like are open-ended words that mean "including, but not limited to," and are used interchangeably therewith. The words "or" and "as used herein mean, and are used interchangeably with, the word" and/or, "unless the context clearly dictates otherwise. The word "such as" is used herein to mean, and is used interchangeably with, the phrase "such as but not limited to".
It should also be noted that in the devices, apparatuses, and methods of the present application, the components or steps may be decomposed and/or recombined. These decompositions and/or recombinations are to be considered as equivalents of the present application.
The previous description of the disclosed aspects is provided to enable any person skilled in the art to make or use the present application. Various modifications to these aspects will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other aspects without departing from the scope of the application. Thus, the present application is not intended to be limited to the aspects shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
The foregoing description has been presented for purposes of illustration and description. Furthermore, the description is not intended to limit embodiments of the application to the form disclosed herein. While a number of example aspects and embodiments have been discussed above, those of skill in the art will recognize certain variations, modifications, alterations, additions and sub-combinations thereof.
Claims (8)
1. The utility model provides a method for acquireing image under microscope, is applicable to the system for acquireing image under microscope, the system includes microscope, image acquisition device, treater and display, image acquisition device installs on microscope third eyepiece, with processor communication connection, the treater is connected with the display, its characterized in that, the method for acquireing includes:
the method comprises the following steps that an image acquisition device acquires a pathological image of a pathological section under a microscope and transmits the pathological image to a processor;
the processor receives a pathological image from the image acquisition device;
the processor performs white balance processing on the pathological image and outputs an adjusted pathological image;
a display receives and displays the adjusted pathology image from the processor.
2. The method of claim 1, wherein the processor performs white balance processing on the pathology image and outputs the adjusted pathology image, and the method includes:
sorting the pixels of the pathological image, and selecting a white reference point;
the RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter; r =255/RN；g=255/GN；b=255/BN；
Adjusting the RGB value R of the pathological image according to the adjusting parametery:Gy:By=(Rx*r)(Gx*g)(Bx*b)；
Outputting the adjusted pathology image.
3. The method according to claim 2, wherein when the white reference point is selected, the pixels of the pathological image in the current field of the microscope are sorted, and after an abnormal bright point is removed, a suitable pixel point is selected as the white reference point.
4. The method of claim 1, wherein the microscope is a microscope with a light source.
5. The method of claim 4, wherein the light source is located between a microscope stage and the camera of the image capturing device.
6. The method of claim 5, wherein the processor performs white balance processing on the pathology image and outputs the adjusted pathology image, and the method comprises:
taking the pathological section away from a microscope objective table, and directly irradiating a camera of the image acquisition device by a light source;
selecting proper pixel points of the image under the microscope visual field as white reference points;
the RGB value R of the white reference pointN:GN:BNComparing the color with a standard white RGB value of 255:255:255 to obtain an adjusting parameter;
r=255/RN；g=255/GN；b=255/BN；
placing the pathological section back to a microscope objective table;
adjusting the RGB value R of the pathological image according to the adjusting parametery:Gy:By=(Rx*r)(Gx*g)(BxB); outputting the adjusted pathology image.
7. An electronic device comprising a processor;
and a memory in which are stored computer program instructions which, when executed by the processor, cause the processor to perform the method of acquiring an image under a microscope according to any one of claims 1 to 6.
8. A computer readable medium having stored thereon computer program instructions which, when executed by a processor, cause the processor to perform the method of acquiring an image under a microscope according to any one of claims 1 to 6.
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| CN113647914A (en) * | 2021-08-23 | 2021-11-16 | 许江珂 | Medical remote laser shooting display system |
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