CN1112680C - Bacteriopurpurin optical signal storing method and its device - Google Patents

Abstract

The present invention utilizes the photochromic characteristic of bacteriorhodopsin (BR) to store information in different molecular states. The present invention is characterized in that the information is excited by three kinds of lasers with different wavelength to realize the write-in, read-out and erasion of the information. The present invention utilizes a near-field optical method to break through the limitation of optical diffraction limit of a traditional optical system and fully perform the function of the optical storage capability of the bacteriorhodopsin. In the device of the present invention, CCD real-time monitoring and real-time information acquisition treatment are carried out so as to improve the signal-noise ratio of information recording and the speed for reading information treatment.

Description

Bacteriopurpurin optical signal storing method

The present invention relates at a kind of known biological optical memory material---the method for canned data on the bacteriorhodopsin,BR belongs to laser, material and area information storage.

The development of informationized society be unable to do without high performance storage medium, simultaneously memory technology also be always most important in the infotech also be one of part with fastest developing speed.In today of computer technology develop rapidly, to the high-speed high capacity memory technology with the growth requirement of Rapid storage technique is very urgent at random.And existing storage medium (as semiconductor memory, disk, CD, tape etc.), (requirement that has closed on the growth of the limit, silicon single crystal and doping process as the optical semiconductor lithography is also more and more higher owing to the performance of self material is very limited.While, element spacing was constantly dwindled, and interelement interference is on the rise along with integrated level improves constantly, and heat dissipation problem also is difficult to solve).Therefore, people replace present recording materials at the new material of urgent searching.

CD is that grow up after microphotography (being suitable for the forties) and magnetic-based storage media (being suitable for the sixties) a kind of brand-new also is the most successful a kind of information storage system.It by laser beam irradiation to the disc (forming) of rotation by protective seam, recording dielectric layer, reflection horizon and substrate, the physics or the chemical change that utilize recording dielectric layer to take place, thus change reflection of light and see through intensity and carry out the record of binary message.Its feature is the storage density height, information capacity is big, the holding time is long.CD can be divided into the three major types type: CD-ROM (ROM), WORM type CD (WORM) but and erasing type CD (EDRAW).Involved in the present invention is EDRAW type CD.The EDRAW CD is different from CD and WORM CD, and its storage is reversible, i.e. write-read and wiping.Present such CD mainly based on magneto-optic effect, can intend phase transformation and photochromic, what present commercial EDRAW CD adopted all is inorganic material.And the storage means that reaches of the present invention is the super-high density optical information storage of carrying out on a kind of biological optical memory material, can be applied to EDROM super-high density CD.

Since this century the eighties, it is found that some biomolecule has the function of excellent photochromic, high speed optoelectronic response and switch storage and self-assembly and self-organization, can satisfy the demand of high speed, high capacity storage and data message parallel processing from now on, thereby rise the upsurge of biomolecular device and Biotic molecule computer research in the world.Halophiles purple membrane photosensitive protein-bacteria rhodopsin (Bacteriorhodopsin is called for short bacteriorhodopsin,BR or BR) is exactly an outstanding representative wherein.

Bacteria rhodopsin be wild Halophiles (Halobacterium, halobium) the two-dimentional crystallization-purple membrane that forms naturally on the cell membrane (Purple membrane, PM) in unique protein ingredient, molecular weight is about 26000.It is organized in salinity than surviving in the high sabkha more than 6 times of seawater, comprise protein-bacteria rhodopsin and phosphatide matrix (protein: phosphatide=3: 1) in the purple membrane, and formation has the trimer of quasicrystal structures, this trimer has the proton pump function of striding film transportation proton in bacterial cell, by absorbing photon, bacteria rhodopsin can be converted into the photopia state from the dark adatpation state, and cause a light cyclic process that has many intermediate states (bR-K-M-N-O-P-bR), stride membranous sub-gradient thereby produce, finish proton and transmit, luminous energy is converted into chemical energy phosphate and ADP are synthesized energy matter ATP from the film of striding of tenuigenin side (Cytoplasmic) to the cell outside (Extracellular).Bacteria rhodopsin is the polypeptide rod that contains 7 α spirals perpendicular to surface of cell membrane, and the polypeptide key strides across film, and its C end is in film, and N holds outside film, and proton is finished by schiff bases and striden the film transmission.Molecule film by the BR material preparation is unique crystallization biological membrane of finding at present, it has unique optical drive proton pump and suitable anti-switching function, and bistable state and rapid photoelectric response characteristic, particularly its good photochromic properties has become the novel focus material of the optical storage and the optical information processing of common concern in the world gradually in the dark tool Development Prospect of optical storage field.

The objective of the invention is to design a kind of bacteria rhodopsin biomolecule super-high density optical storage method, in order to be implemented in the high capacity on new bio storage medium-bacteria rhodopsin, the information stores of super-high density based on the photochromic mechanism of biomaterial.

The present invention system utilizes the photochromic characteristic of bacteriorhodopsin,BR (BR), and information is stored with the different molecular attitude, it is characterized in that exciting with three kinds of different wavelength of laser, realizes writing of information, reads and wipes.B attitude (ground state) representative " 0 " or " 1 " with the bacteriorhodopsin,BR molecule, with one of them the intermediate state P attitude representative " 1 " or " 0 " in photochromic, laser radiation with wavelength 620～670nm, the molecule that makes bacteriorhodopsin,BR is realized writing of information from " 0 " of B attitude or " 1 " or " 0 " of " 1 " → P attitude; Laser radiation with wavelength 470--500nm, make " 1 " or " 0 " of bacteriorhodopsin,BR branch Ziren P attitude return " 0 " or " 1 " of B attitude, realize wiping of information, with being positioned at outside the P attitude molecular absorption band, the light of wavelength is as reading light in the B attitude molecular absorption band, and reading optical wavelength is 585--610nm.The exciting light optimal wavelength that writes, reads, wipes is respectively 640nm, 594nm, 490nm.Can adopt the near field optic method to break through the restriction that conventional optical systems is subjected to optical diffraction limit, give full play to the optical storage ability of bacteriorhodopsin,BR.The device of Bacteriopurpurin optical signal storing method is characterized in that by the light path pass order write section branch comprises light source, beam expander, collimating mirror, spatial light modulator, image optical device and monitor; Read part and comprise light source, beam expander, collimating apparatus, image optical collimation and figure image reader; Wipe part and comprise light source, beam expander, collimating apparatus, bacteriorhodopsin,BR molecule film memory device places write section to divide imaging optical devices imaging surface place, and this position also reaches the object plane device of reading imaging optical device between image optical device partly and the collimating apparatus after wiping the part collimating apparatus.The light source that writes, reads, wipes three parts is respectively the laser instrument that output wavelength is 620--670nm, 585--610nm, 470--500nm.In the light path that writes, reads, wipes three parts, be provided with activity shading baffle plate respectively.The monitor that writes part is the real-time monitoring device of CCD device, computing machine, display formation, and the figure image reader of reading part is the common device that constitutes of CCD device, image collection card, pictorial data process software and computing machine.

Drawings and Examples:

Fig. 1 is the absorption spectrum curve of BR molecule B, P attitude;

Fig. 2 is the device synoptic diagram of BR molecule optical information storage means;

Fig. 3 is a molecular film optical storage device front view (FV).

Bacteriorhodopsin,BR is as a kind of photochromic material of biomolecule, it is photochromic mainly be utilize in its light circulation ground state and wherein the mutual conversion between some intermediate state realize.Bacteriorhodopsin,BR have many other photochromic materials incomparable advantage: as utilize photochromic characteristic between the different intermediate states of BR molecule, can obtain the optical storage performance of different storage lifes, thereby serve many purposes, particularly the long-life storage characteristics possesses the ideal conditions that is used as optical storage media that can be erasable repeatedly from now on especially; BR light cycle index can reach 107 times, and access times are close to unlimited; Can be assembled into molecular device and realize highdensity information stores; By engineered dna modification and material biology, chemical modification, can change its some photochemical properties according to people's will.In addition, BR draws materials easily, is easy to a large amount of breedings simultaneously and cultivates, and is with low cost; Above advantage makes bacteriorhodopsin,BR be expected to become a kind of important information storage material.The optical storage of BR has utilized its photochromic characteristic exactly, information is stored with the different molecular attitude, as the B attitude as 0 storage, and another one attitude in photochromic as 1 storage, and carry out the erasable of information and read with the light of different colours.

Can form stable photochromicly between the B attitude (ground state) of bacteriorhodopsin,BR molecule and the new attitude P of the branch attitude, under near the irradiation of ruddiness (the 640nm wavelength), the BR molecule can be excited to P attitude (representing 1).The absorption peak of P attitude is at 490nm, and the P attitude will be returned BR attitude (representing 0) under the irradiation of 490nm light.Use the light of above-mentioned two kinds of wavelength can realize writing (640nm) and wiping (490nm) of information.

Because the BR molecule not optical absorption band between the homomorphism exists certain overlappingly, this has brought big difficulty for reading of writing information.Both needed to read the information that light can extract record reliably, avoided change simultaneously again, promptly do not had reading of damage type writing information.

Fig. 1 is the absorption spectrum curve of BR molecule P attitude.As can be seen its to absorb bandwidth be 450～550nm spectral range, need to adopt light away from bacteriorhodopsin,BR P attitude absorption peak wavelength as reading light as far as possible, be damaged to avoid writing information, thereby cause the losing of information after the repeatedly read operation; Reading light simultaneously also needs to satisfy by the enough signal to noise ratio (S/N ratio)s in different B R molecular state (B attitude and P attitude) back, with reacting record information exactly.The absorption peak of BR attitude is about 570nm, and absorption wide-band is near 540～610nm.The light of absorption spectrum curve in this wavelength coverage of Figure 1B R molecule B attitude and P attitude will be by B attitude molecule absorption, the luminous energy of passing through will be weakened, the BR molecule that is in the P attitude is as absorbing this incident light, by after light intensity will be constant substantially, this difference will be screened by the detection system of back.So, need to adopt the light conduct of wavelength in the absorption band of the outer and B attitude molecule of P attitude molecular absorption band to read light.Consider the contrast of read output signal, select near the wavelength of 594nm, can satisfy the needs of not damaged read operation as reading light.

The optical storage of bacteriorhodopsin,BR is based on that molecular level takes place photochromic the realization, therefore the limit of canned data can reach molecular level on principle, i.e. information that molecule can be stored a bit, and bacteriorhodopsin,BR molecular geometry yardstick is the dust level, promptly 10 ^-10The rice magnitude.Therefore bacteriorhodopsin,BR can reach 10 as storage medium ³～10 ⁴Tb/cm ²Ultrahigh-Density Data Storage (present information storage capability is 10Mb/cm ², the two differs 10 ⁶Doubly).But traditional optical system is subjected to the restriction of optical diffraction limit, and the focus that light beam focuses on can't promptly can't reach nanometer or higher resolution characteristic less than the length of incident wavelength.Therefore also can't give full play to the ability of bacteriorhodopsin,BR molecule storage.The application adopts optical means-near field optic method of a kind of advanced person, can break through the restriction of optical diffraction limit, gives full play to the ability of bacteriorhodopsin,BR optical storage.

Near field optic is that research object is with interior optical phenomena apart from wavelength of body surface along with science and technology advances a novel crossed subject in the optical field that is occurred to small size and lower dimensional space.The near field optic microscopy is a kind of novel ultrahigh resolution optical microscopy.In recent years, along with research, development and the application of Near-field Optical Microscope, the near field optic microscopy has all obtained breakthrough progress in theory with in the practice.Conventional optical microscope is to utilize lens that the amplification of object is made, and enlargement factor can reach 1,000 times, but its detecting element all is arranged in the far field much larger than optical wavelength.Because the diffraction of light effect, the raising of its resolution is subjected to the restriction of Rayleigh limit and optical system numerical aperture, and measuring point generally is not more than half wavelength (δ x 〉=0.6 λ/NA).If will obtain bigger enlargement factor, high resolving power more, there is the difficulty that is difficult to overcome technically.The mechanism of near field optic method is: the outer field distribution of body surface generally is divided into two zones: a part is apart from the body surface near-field region of several wavelength only; Another part be outside near-field region to the infinite distance this far-field region.Existing in near-field region can have again to only limit to wavelength of body surface with interior non radiation field to the radiation field of propagating at a distance, and wherein non radiation field is present in body surface, and intensity is decay rapidly along with the increase of leaving the body surface distance.In the far-field region, only there is radiation field, there is not non radiation field.Compare with conventional optical systems, the maximum with it difference of near-field optical systems is: a) replaced the conventional optical microscope head with nano level optical probe.B) be controlled in wavelength of sample surfaces at the detection process middle probe, promptly probe is positioned at the near-field region of sample surfaces.And there is the non radiation field of belongings surface fine structure information in the near-field region of sample surfaces, so, be in the optical information that the interior probe of near-field region may detect submicron order, like this, just broken through the restriction of Rayleigh diffraction limit, its resolution can be much smaller than the size of lambda1-wavelength, and near-field optical systems can have been accomplished the level of nano-resolution at present.Therefore, the optical resolution in conjunction with the super diffraction limit of the characteristics of bacteriorhodopsin,BR molecule canned data and near field optic just can be implemented in the super-high density optical information storage on the bacteriorhodopsin,BR material.

The optical information reading and writing of BR and erasing apparatus have comprised writing, reading and wipe three parts as shown in Figure 2: write part and be made up of light source 1, optically filtering, beam expander 4, collimating apparatus 5, spatial light modulator 6, image optical device 7, the real-time monitor 14 of CCD.Light source can adopt near the laser instrument of wavelength 640nm, and as the fiber-coupled diode laser instrument of the SDL-07470-P5 type LD pumping of U.S. PRELIMINARY company, its output wavelength is 650nm, and the optical fiber of 400 μ m passes light beam; Optically filtering is mainly used to improve and writes uniformity of light; The CCD monitoring system is used for the accurate adjustment of imaging light path, comprises a CCD device and a display.Information to be written can be provided by spatial light modulator (or having the slide that needs recorded information).Read part and expand bundle and collimating apparatus the 10,11, the 12nd,, expand bundle, collimation the second time of carrying out in order to adjust beam diameter by Yellow light laser (about wavelength 594nm) 2, optically filtering 9 as gold-tinted He-Ne laser instrument.Image optical device 13, figure image reader form 14,15,16.The figure image reader constitutes 15 by CCD device 14 and image collection card, pictorial data process software.Can directly the information that reads be converted to computer graphics metafile by image collection card, again through the pictorial data process software with information via analysis and processing and be stored in the computing machine.Baffle plate 21 is when carrying out a kind of operation among Fig. 2, and the two-way baffle plate inserts any part that can place light path in addition.The signal to noise ratio (S/N ratio) of recorded information mainly from record, read the spatial resolution of optical system and the pixel size of CCD device, particularly latter's influence is in the highest flight; Adopt the low light level to read and to improve signal to noise ratio (S/N ratio), but the sensitivity and the dynamic range of CCD device are had relatively high expectations.

Wiping part is made of laser instrument 3 and beam expander 17, collimating apparatus 18.Wiping its output wavelength of used laser instrument near 490nm, is the Beamlok2085-15 laser instrument of Spectra-Physics as Argon ion laser, adopts the 488nm single line laser of its output.

Recording of information: information at first to be recorded can be by in the computing machine input space photomodulator 6, write light source and pass through spatial light modulator through filtering, expansion bundle with after collimating, through the image optical device with information imaging to be recorded on bacteria rhodopsin molecule film memory device 8, the front view (FV) of this device is (this device can be chosen the finished product bacteriorhodopsin,BR molecule film of having sold both at home and abroad) as shown in Figure 3, at this moment, recording of information is finished; Reading of information: read light through after the filtering, beam-expanding collimation by behind catoptron 19 and the prism 20, becoming a branch of collimation electrical lighting light beam is incident upon on the bacteria rhodopsin molecular device 8, the information that light will carry record of reading by device, and the imaging of process image optical device is on the photosurface of CCD, CCD obtains sense information, by the imgae processing software in image collection card and the computing machine recorded information is converted into computer graphics metafile, the readout of information is so far finished.Read the beam diameter of light and can control its size, thereby be fit to the bacteria rhodopsin device of different size by regulating beam expander and collimating apparatus.Wiping of information: by wiping light that light source sends, be radiated on the bacterial rhodopsin molecular membrane of recorded information, can wipe the information that has write down rapidly through after expanding bundle and collimation.At this moment, the bacterial rhodopsin molecular membrane device can carry out writing next time, read operation again easily.

As a kind of novel, outstanding optical memory material, BR has the unrivaled advantage of many other storage mediums: (1) can be assembled into molecular device, thereby can realize the information stores needs of super-high density; (2) utilize photochromic characteristic between the different intermediate states of BR molecule, can obtain the optical storage performance of multiple storage life character, thereby serve many purposes, particularly the long-life storage characteristics possesses the ideal conditions that is used as high-density biology CD that can be erasable repeatedly from now on especially.Can reach 10 with the time cycle index ⁷Inferior, the repetitive erasing number of times is close to unlimited.(3) by different biological and chemical group of molecules packing techniques,, the molecules film of different molecular spread pattern can be obtained, the molecule arranging structure of various needs can be obtained as LB membrane technology, electro-deposition techniques etc.(4) by engineered dna modification and material biology, chemical modification, can change its some photochemical properties according to people's will, as prolong some intermediate state life-span, make a certain intermediate state become structure (as 9-cis) of stable state or the like.Make us obtain better material behavior, so that be suitable for the application of different occasions.(5) the BR molecular film can carry out mixing of special process with high molecular polymer, thereby is strengthened greatly at aspects such as optical homogeneity, molecule arranging structure and physical strengths, uses thereby be more suitable for coml.(6) the BR material is taken from nature salt Tanaka's Halophiles, draws materials easily, be easy to a large amount of breedings simultaneously and cultivate, so cost is very cheap, has important commercial and is worth.(7) the BR material has fabulous stability, and for example at 140 ℃ high temperature, desiccation and pH value are a kind of ideal engineering materials near still biologically active and photolytic activity under 0 the environment; (8) the BR material is the natural bacteria bioprotein, and its use can not cause damage and pollute nature and people, is the optical function material of a kind of pure " green ", meets the requirement of human kind sustainable development.Because bacteria rhodopsin possesses above plurality of advantages, therefore, very be suitable as a kind of super-high density, vast capacity and erasable repeatedly number of times and be close to unlimited optical disk medium of new generation.(9) utilize photochemical reaction mechanism different in the circulation of bacteriorhodopsin,BR light and the SPECTRAL DIVERSITY between the bistable state to absorb respectively, realized writing (B (0) → P (1)), wipe (P (1) → B (0)) and reading (B and P attitude absorb in the difference of 594nm) of optical information; This reading method belongs to undamaged information read method; From the bistable absorption Spectrum characteristic of bacteriorhodopsin,BR, both considered the signal to noise ratio (S/N ratio) of sense information, can satisfy simultaneously reading of recorded information (P attitude) no erasure effect; (10) real-time information collection, processing and the monitoring that combine with computing machine of CCD; Adopting high-resolution CCD device in real time sense information to be sent to handles in the computing machine and analyzes; By video output, can monitor in real time the situation of sense information in real time simultaneously by monitor; Can gather, analyze, handle and store sense information according to user's setting by the data acquisition system (DAS) that computing machine, pictorial data process software and image collection card constitute; (11) CCD that guarantees the recorded information signal to noise ratio (S/N ratio) monitors in real time; When recorded information, CCD device and monitor have constituted the real-time monitoring device of digital data recording system, when adjusting writing system, can provide information to write situation in real time, intuitively, be more convenient for practical operation and the best recorded information signal to noise ratio (S/N ratio) of acquisition.The present invention that need illustrate only relates to reading and writing, method for deleting and the device of optical information, and molecular film and device thereof then are not the application's scopes, and it is studied and disclosed by the related personnel of field of biological molecule.

Claims (3)

1, Bacteriopurpurin optical signal storing method, system utilizes the photochromic characteristic of bacteriorhodopsin,BR (BR), information is stored with the different molecular attitude, adopt three kinds of different wavelength of laser to excite, the writing, read and wipe of realization information, it is characterized in that ground state (B attitude) representative " 0 " or " 1 " with the bacteriorhodopsin,BR molecule, with one of them intermediate state in photochromic (P attitude) representative " 1 " or " 0 ", laser radiation with wavelength 620-670nm, the molecule that makes bacteriorhodopsin,BR is realized writing of information from " 0 " of ground state or " 1 " or " 0 " of " 1 " → intermediate state; With the laser radiation of wavelength 470-500nm, make the bacteriorhodopsin,BR molecule return " 0 " or " 1 " of ground state from " 1 " or " 0 " of intermediate state, realize wiping of information, with being positioned at outside the intermediate state molecular absorption band, light is read in the conduct of the light of wavelength in the ground state molecule absorption band.

2, Bacteriopurpurin optical signal storing method according to claim 1, it is characterized in that reading optical wavelength is 585-610nm.

3, Bacteriopurpurin optical signal storing method according to claim 1 and 2 is characterized in that the optical maser wavelength that writes, reads, wipes is respectively 640nm, 594nm, 490nm.

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