CN111264272A - Method for activating small molecules by cold resistance potential of plants - Google Patents
Method for activating small molecules by cold resistance potential of plants Download PDFInfo
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- CN111264272A CN111264272A CN202010117517.4A CN202010117517A CN111264272A CN 111264272 A CN111264272 A CN 111264272A CN 202010117517 A CN202010117517 A CN 202010117517A CN 111264272 A CN111264272 A CN 111264272A
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- plants
- nerolidol
- cold resistance
- plant
- methyl salicylate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention provides a method for activating small molecules by cold resistance potential of plants, which comprises the following steps: the plants are sprayed with nerolidol and methyl salicylate from the exogenous sources. The method adds nerolidol and methyl salicylate to the plants by means of exogenous spraying, and detects the cold resistance of tea trees and model plants; the spraying of nerolidol and methyl salicylate from exogenous sources to improve the cold resistance of plants has the advantages of convenient operation, low cost, no toxicity, no harm and the like.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a method for activating small molecules by cold-resistant potential of plants.
Background
Low temperature is an important environmental factor affecting plant yield and distribution. In the life history of plants, such as rice seedling raising in spring, the threatens of low temperature always occur in a period from sowing to seedling emergence in a dry field and a period after seedling emergence and a lifetime of crops which are growing normally. The effects of low temperature are mainly cold damage and freeze damage. Low temperature stress not only causes a reduction in plant yield but also, in severe cases, causes death of the plant.
After the plants are subjected to cold damage, the cell solution is frozen and the wall membrane is separated and damaged. Overwintering crops such as winter wheat, rape, certain perennial root pasture, feed and the like are frozen and damaged, and large-area dead seedlings are mainly shown. After the fruit trees and the forest trees are frozen, the xylem of the branches turns brown and even dies due to dryness. The frost damage of melon seedlings results in long-term seedling recovery and even seedling hardening, and the heavier plants are frozen to become black and withered. Frost damage freezes solutions in plant tissues into ice, causing injury or death. Late frost is easy to occur after spring sowing crops and fruit trees bloom and overwintering crops turn green, and early frost is easy to occur when autumn crops and fruits are not mature and outdoor vegetables are not harvested.
At present, the frost resistance of plants is improved basically through physical methods such as greenhouse planting and the like, but for a plurality of planting areas, perennial economic crops can greatly increase the cost of products.
Tea is considered the most popular non-alcoholic beverage in the world. While such evergreen perennial plants can grow in a variety of different agricultural climatic regions, tea plants can grow in tropical to subtropical climates. Due to local climate changes, tea plants have to cope with low temperatures in winter. Low temperature is one of the most critical environmental factors that limit its growth, survival and geographical distribution. In early spring every year, fresh leaves and tender shoots of tea leaves can be picked off and used for making tea. Because young leaves and buds have poor tolerance to low temperatures, sudden frost in early spring often causes cold stress, affecting the economic yield of tea.
Tea leaves contain abundant volatile aroma substances, and volatile aroma substance-mediated interaction in plants under low-temperature stress of tea trees is rarely studied and is experimentally challenging.
Disclosure of Invention
In view of the above technical problems, the present invention provides a method for activating small molecules by the cold-resistant potential of plants.
The specific technical scheme is as follows:
a method for activating small molecules against cold potential in plants comprising the steps of: spraying nerolidol to the plants from the exogenous sources. The concentration of nerolidol is 0.5 x 10-9mM～0.5*10-6And mM. The concentration of methyl salicylate is 0.2 x 10-9mM～0.2*10-6mM。
The method for activating the small molecules by the cold resistance potential of the plant provided by the invention has the following beneficial effects:
this patent adds nerolidol and methyl salicylate to the plant through the means of exogenous spraying, detects the anti cold ability of tea tree and mode plant. The spraying of nerolidol and methyl salicylate from exogenous sources to improve the cold resistance of plants has the advantages of convenient operation, low cost, no toxicity, no harm and the like.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
Wherein:
FIG. 1A is a chlorophyll fluorescence diagram of a plant;
FIG. 1B is a box diagram showing the Fv/Fm values of tea leaves after exogenous addition of nerolidol;
FIG. 1C is a box diagram showing the Fv/Fm values of tea leaves after exogenous addition of nerolidol.
FIG. 2A is a chlorophyll fluorescence plot after the addition of nerolidol;
FIG. 2B is a graph of chlorophyll fluorescence after addition of methyl salicylate;
FIG. 2C is the fv/Fm value after addition of nerolidol;
FIG. 2D shows the fv/Fm values after addition of nerolidol.
FIG. 3A shows the Malondialdehyde (MDA) content and osmotic pressure of tea plant after exogenous addition of nerolidol;
FIG. 3B shows the superoxide dismutase (SOD) content of tea plant after nerolidol is added from the external source;
FIG. 3C shows the Peroxidase (POD) content of tea plant plants after exogenous addition of nerolidol;
FIG. 3D shows the Malondialdehyde (MDA) content and osmotic pressure of the tea plant after exogenous addition of methyl salicylate;
FIG. 3E shows the superoxide dismutase (SOD) content of tea plants after exogenous addition of methyl salicylate;
FIG. 3F tea plant Peroxidase (POD) content following exogenous addition of methyl salicylate.
FIG. 4A shows control and hydrogen peroxide (H) in tea plant plants sprayed with nerolidol2O2) Content (c);
FIG. 4B shows control and spray applicationSuperoxide anion (O) in tea plant after nerolidol2) Content (c);
FIG. 4C shows hydrogen peroxide (H) in tea plants after control and methyl salicylate spray application2O2) Content (c);
FIG. 4D shows superoxide anions (O) in control and sprayed with methyl salicylate2) And (4) content.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise. It is to be understood that the scope of the invention is not to be limited to the specific embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.
Unless otherwise indicated, the experimental procedures for the specific conditions in the following detailed description are generally in accordance with conventional procedures and conditions within the skill of the art, which are fully explained in the literature.
A method for activating small molecules against cold potential in plants comprising the steps of: spraying nerolidol to the plants from the exogenous sources.
Culturing whole plant (tea seedling, Arabidopsis thaliana, tobacco) in 10L transparent jar, placing a small ball of absorbent cotton in the jar, adding nerolidol standard samples (0.5 × 10) with different concentrations onto the absorbent cotton-9mM、0.5*10-8mM、0.5*10- 7mM、0.5*10-6mM) and methyl salicylate standard (0.2 x 10)-9mM、0.2*10-8mM、 0.2*10-7mM、0.2*10-6mM)。
The control group was a solvent that dissolved the same volume of dissolved standard. The opening of the tank is sealed, and the air vent is sealed by active carbon.
Culturing at room temperature for 9 hr, taking out the plant, freezing in ultralow temperature refrigerator (-5 deg.C) for 2 hr, and taking chlorophyll fluorescence image by chlorophyll imaging system.
The blue color of the leaf represents normal leaf, and the damage degree gradually deepens from green to yellow to black, as shown in the following chart color card. The larger the Fv/Fm value, the smaller the damage degree and the stronger the cold resistance.
FIGS. 1A-1C show that the cold resistance of tea tree can be improved by adding nerolidol and methyl salicylate with different concentrations from external sources, and CK is a control; t1 is added at a concentration of 0.5 x 10-9mM/0.2*10-9Plants after mM; t2 is added at a concentration of 0.5 x 10-8mM/0.2*10-8Plants after mM; t3 is added at a concentration of 0.5 x 10-7mM/0.2*10-7Plants after mM. FIG. 1A is chlorophyll fluorescence diagram of plant, and FIGS. 1B and 1C box type diagram is Fv/Fm value.
2A-2D show exogenous addition of 0.5 x 10-8mM/0.2*10-8mM nerolidol and methyl salicylateThe ester can improve the frost resistance comparison chart of arabidopsis and tobacco; FIG. 2A is a chlorophyll fluorescence plot after the addition of nerolidol; FIG. 2B is a graph of chlorophyll fluorescence after addition of methyl salicylate; FIG. 2C is the fv/Fm value after addition of nerolidol; FIG. 2D shows the fv/Fm values after addition of nerolidol.
FIGS. 3A-3F show the exogenously added tea plant Malondialdehyde (MDA) content (in FIGS. 3A/3D), osmotic pressure (in FIGS. 3A/3D), SOD (in FIGS. 3B/3E), and POD (in FIGS. 3C/3F). MDA is one of the most important products of membrane lipid peroxidation, and the generation of MDA can also aggravate the damage of a membrane, so the content of MDA can represent the damage degree of the membrane under low temperature, and the MDA content of a plant sprayed with nerolidol is obviously lower than that of a control group. Osmotic pressure (osmotic pressure) is also a physiological index reflecting plant resistance, and the higher the osmotic pressure value is, the lower the cold resistance is. SOD and POD contents are also important physiological indexes for embodying the cold resistance of plants, and the higher the concentration of enzyme is, the stronger the cold resistance is. According to the indexes, the exogenous spraying of nerolidol and methyl salicylate can reduce the content of malondialdehyde, reduce osmotic pressure, and improve the activity of SOD POD enzyme, thereby improving the cold resistance of plants.
Fig. 4A-4D show the contents of hydrogen peroxide (fig. 4A/4C) and superoxide anion (fig. 4B/4D) in the tea plant body after nerolidol and methyl salicylate were sprayed and compared, which are the main active oxygen substances causing plant damage, and the contents of hydrogen peroxide and superoxide anion in the plant body after nerolidol was sprayed are dramatically increased when the plant is damaged by low temperature, while the contents of hydrogen peroxide and superoxide anion in the plant body after nerolidol was sprayed are significantly lower than those in the control group, which indicates that nerolidol can improve the cold resistance of the plant by eliminating the excessive active oxygen in the body.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present application, and shall be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Claims (2)
1. A method for activating small molecules against cold potential in plants comprising the steps of: the plants are sprayed with nerolidol and methyl salicylate from the exogenous sources.
2. The method of claim 1, wherein the concentration of nerolidol is 0.5 x 10-9mM～0.5*10-6mM; the concentration of methyl salicylate is 0.2 x 10-9mM～0.2*10-6mM。
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010117517.4A CN111264272A (en) | 2020-02-25 | 2020-02-25 | Method for activating small molecules by cold resistance potential of plants |
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| CN202010117517.4A CN111264272A (en) | 2020-02-25 | 2020-02-25 | Method for activating small molecules by cold resistance potential of plants |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906201A (en) * | 2017-04-10 | 2017-06-30 | 武汉大学 | A kind of Terpene synthase for producing nerolidol and its application |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906201A (en) * | 2017-04-10 | 2017-06-30 | 武汉大学 | A kind of Terpene synthase for producing nerolidol and its application |
Non-Patent Citations (2)
| Title |
|---|
| 冯斗: "水杨酸甲酯对香蕉苗抗寒生理特性的影响", 《中国南方果树》 * |
| 无: ""我学者首次发现香气有助植物抗寒"", 《中国食品学报》 * |
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