CN111263587A - Use of isotianil for combating panama disease - Google Patents

Abstract

The present invention relates to the use of isotianil (formula (I)) (I) for controlling panama disease in plantago plants. Furthermore, the invention relates to a method for controlling panama disease by treating plants of the family musaceae with isotianil (I).

Description

Use of isotianil for combating panama disease

The present invention relates to the use of Isotianil (IST) (formula (I)) for the control of panama disease in plantago plants.

Furthermore, the present invention also relates to a method for controlling panama disease by treating isotianil or a formulation comprising isotianil (formula (I)) in plantaaceae plants with them.

Furthermore, the invention relates to the mixture of isotianil with at least one other active ingredient selected from fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, safeners, host defense inducers, soil improvement products or plant stress-alleviating products (e.g. Myconate) for the treatment of panama disease, for example to broaden the spectrum of action or to prevent the development of resistance.

In a preferred embodiment, the present invention relates to a mixture of isotianil with at least one further active ingredient selected from the group consisting of Fosetyl-aluminium (Fosetyl-Al), and mono-and di-sodium phosphites, mono-and di-potassium phosphites, and mono-and di-ammonium phosphites (such as Phostrol) for controlling panama disease in plantago plants.

Compounds of the formula (I) are known in particular from WO 99/024413, WO 2006/098128, JP 2007-84566 and WO 96/29871.

Invention of the invention

Panama disease is an invasive plant disease of banana plant roots caused by the fungal pathogen Fusarium oxysporum (Fusarium oxysporum), particularly Fusarium oxysporum cubense (F0c)) race 1 and race 4. It is the most destructive disease in bananas. For example, in the 50's of the 20 th century, panama disease caused a sharp decrease in banana yield in most commercial mackerel (gross Michel), the major variety of bananas at that time. Today, new strains of panama again threaten the yield of the most popular cultivar today, the Cavendish (Cavendish) cultivar.

Fusarium fungi enter the roots of plants and diffuse through the xylem vessels of the plants. Fungi destroy the vascular system of plants, causing leaves to yellow and wither, eventually leading to plant death.

To date, 4 races of this pathogen affect banana crops worldwide. Its "race 1" has spread to the philippines and indonesia, where the more aggressive "race tropical race 4" is now spreading. "race 1" also spreads in africa and australia. It has not yet arrived in latin america, but it is only a matter of time when panama disease will also destroy banana plantations in the area. This would cause significant economic losses and endanger the survival of the relevant banana growers.

To date, fusarium pathogens are resistant to fungicides and no chemical solution is available.

In WO 2010/037482 isotianil derivatives are described as examples for controlling microbial and animal pathogens in plantago, only melasma (Mycosphaerella fijiensis).

It has now been found that isotianil and mixtures of isotianil with at least one other active ingredient are particularly suitable for controlling panama disease on plantago plants.

Therefore, a first subject of the present invention is the use of isotianil for controlling panama disease in plantaaceae plants.

Therefore, another subject of the present invention is the use of isotianil for controlling panama disease in plantain plants.

Another subject of the invention is a method for controlling panama disease in plantago plants, characterized in that isotianil is used to treat plantago plants.

Another subject of the present invention is the use and the method as described above, wherein isotianil is used in combination with at least one other active ingredient selected from fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, safeners, host defense inducers, soil improvement products or plant stress-alleviating products (e.g. Myconate), for example to broaden the spectrum of action or to prevent the development of resistance.

A preferred embodiment of the present invention is the above use and method wherein Fusarium oxysporum cubeba specialized type No. 1 race or No. 4 race causes Panama disease.

Another preferred embodiment of the present invention is the above use and method, wherein isotianil or a combination of isotianil and at least one further active ingredient is administered, wherein the further active ingredient is preferably selected from the group consisting of fosetyl-aluminum, the mono-and disodium salts of phosphorous acid, the mono-and dipotassium salts of phosphorous acid and the mono-and diammonium salts of phosphorous acid, more preferably fosetyl-aluminum.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation application, preferably 2.5 to 0.5g fosetyl-aluminum/plant and 0.035 to 0.015 IST/plant, more preferably 2.0 to 1.0g fosetyl-aluminum/plant and 0.03 to 0.02g IST/plant, even more preferably 2.0 to 1.0g fosetyl-aluminum/plant and 0.03 to 0.02g IST/plant and most preferably 1.6g fosetyl-aluminum/plant and 0.024g IST/plant are used every 30 days, more preferably every 14 days.

Another preferred embodiment of the present invention is the above use and method, wherein isotianil is used in combination with fosetyl-aluminum in a ratio of from 1 to 60 to 1 to 70, in% by weight.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation application, preferably 0.45 to 0.1g fosetyl-aluminum/plant and 0.04 to 0.015g IST/plant, more preferably 0.4 to 0.15g fosetyl-aluminum/plant and 0.04 to 0.02g IST/plant, even more preferably 0.35 to 0.25g fosetyl-aluminum/plant and 0.035 to 0.025g IST/plant and most preferably 0.28g fosetyl-aluminum/plant and 0.028g IST/plant are used every 30 days, more preferably every 14 days.

Another preferred embodiment of the present invention is the above use and method, wherein isotianil is used in combination with fosetyl-aluminum in a ratio of from 1 to 5 to 1 to 15, in% by weight.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation application, preferably from 0.035 to 0.015g IST per plant, more preferably from 0.03 to 0.02g IST per plant, even more preferably from 0.03 to 0.02g IST per plant and most preferably 0.024g IST per plant per day, more preferably every 30 days, more preferably every 14 days.

Another preferred embodiment of the present invention is the above use and method, wherein the method used is drip irrigation application, preferably 0.04 to 0.015g IST per plant, more preferably 0.04 to 0.02g IST per plant, even more preferably 0.035 to 0.025g IST per plant and most preferably 0.028g IST per plant is used every 30 days, more preferably every 14 days.

Definition of

The musaceae family is composed in particular of the following species: musa acuminata (Musa acuminata), Musa paradisiana (Musa balbisiana), Musa acuminata (Musa acuminata colula) (having the varieties "dwarf Cavendish (DwarfCavendish)", "Giant Cavendish (GiantCavendish)", and "big Michel (Gros Michel)), dwarf banana (Musa cavendishi lamb. ex Paxt.), Musa malaceensis Ridl., Musa angnsignep, Musa aurantiaca, Musa (Musa basisanina), Musa semifia Lour, Musa bakiii F. mu. cell, Musa basjo, Musa sorangii, Musa sibirica, Musa, Musa banana (Musakusanaria), Musakusana, Musakura, Musa red (Rosa Mikania Michelia), Musajou banana.&Drude), Musa alinnsaya, Musa saccarii, Musa boman, Musa

Musabbukensis, Musa campestis, red bananas (Musa coccinea Andrews), red bananas (Musa uranoscopos Lour), Musa exotica Valkyo, Musa fitzalanii, Musa flavida, Musa gracilis, Musahirta Becc, Isaria mussakayama (Musa insularum Hayata)Musa jackeyi, Musa johnsii, plantain (Musa laminariensis), Musa loldensis, Musa macleayi, Musa monicola, Musa muluuensis, canna (Musa paracoccia), Musa peekelii, Musa Pigmaea Hotta, Arjona (Musa rubra), Musa laccensis, Musa spondida A.chev., Musa suratii, Musa abaca (musatexilis): manila bananas (Abac a), Japanese hardy or fibre bananas, bananas (Musatoglytarum), Musa tubericula, Musa violasches, Musa basjonas (Musa ingens), Musa paradisiaca sapientm, Musa paradisiaca norma, and hybrids of these species.

Examples of fungi of the genus Fusarium causing panama disease in plants of the family musaceae are Fusarium (Fusarium spp), such as Fusarium xanthum (Fusarium pallidosum), Fusarium solani (Fusarium solani) anamorph, Fusarium flagellatum (necatria haematococca), Fusarium oxysporum (Fusarium oxysporum), Fusarium moniliforme (Fusarium moniliforme) idiomorph: gibberella fujikuroi (Gibberella fujikuroi), Fusarium oxysporum cubeba speciality (Fusarium oxysporum f.sp. cubense (Foc)), particularly race nos. 1 and 4 of Foc, more particularly race No. 4 of Foc.

According to the invention, the isotianil or the isotianil mixture described above is used in particular against Fusarium fungi causing panama disease in plants of the family musaceae, said fungi being Fusarium (Fusarium spp), such as Fusarium xanthum (Fusarium pallidosum), Fusarium solani (Fusarium solani) anamorph, Fusarium flagellatum (necator), Fusarium oxysporum (Fusarium oxysporum), Fusarium moniliforme (Fusarium moniliforme) idiotype: gibberella fujikuroi (Gibberella fujikuroi), Fusarium oxysporum cubeba speciality (Fusarium oxysporum f.sp. cubense), particularly race nos. 1 and 4 of Foc, more particularly race No. 4 of Foc.

Isotianil may, if appropriate, be present in a mixture of a plurality of possible isomeric forms, in particular stereoisomers, such as optical isomers.

Therefore, isotianil can be used for protecting plants from the above pathogens or for delaying the attack/symptoms of the above pathogens within a certain time after the treatment. The time for providing protection after treatment of the plants with the active substances is generally from 1 to 30 days, preferably from 1 to 14 days. Depending on the application form, the accessibility of the active substance to the plants can be controlled in a targeted manner.

According to the invention, the good plant tolerance of isotianil at the concentrations required for controlling plant diseases allows the treatment of above-and underground plant parts, vegetative propagation material and soil.

According to the present invention, all plants of the family Musaceae can be treated. In the context of the present invention, plantain is understood to mean all plant parts and plant populations, such as desired and undesired wild plants or crop plants (including naturally occurring crop plants). The crop Plant may be a planta Plant obtainable by conventional breeding and optimization methods or by biotechnological and recombinant methods or a combination of these methods, including transgenic planta plants and including Plant varieties which may and may not be protected by the Rights of Plant Breeders (Plant Breeders' Rights), such as mackawa, cavendish, Dwarf chiese, Enano, Caturra, cavendish, graneno, grand Naine, williams hybrid, Valery, Robusta, Poyo, Lacatan, Pisang masak hijau, Monte cro, borond. Plant parts are intended to mean all above-and below-ground parts and organs of plants, such as herbs (herb), pseudostems, buds, leaves, bracts, leaf sheaths, petioles, leaves, flowers and roots, examples which may be mentioned being leaves, needles, stalks, stems, flowers, fruit bodies, fruits, banana clusters (bananas), bunches (bunches) and seeds as well as roots, tubers, rhizomes, lateral branches, trematodes, secondary plants (secondarygrowth). Plant parts also include crop material (crop material) and vegetative and generative propagation material, for example cuttings, tubers, rhizomes, shoots and seeds.

As already mentioned above, all plantain plants can be treated according to the invention. In a preferred embodiment, plant species and plant varieties and parts thereof found in the wild environment or obtained by conventional biological breeding methods (such as crossing, meristem culture, micropropagation, somatic embryogenesis, direct organogenesis or protoplast fusion) are treated. In another preferred embodiment, transgenic plantain plants and plantain plant varieties (genetically modified organisms) obtained by recombinant methods, if appropriate in combination with conventional methods, are treated, for example by particle bombardment and micropropagation transformation of Agrobacterium or embryonic cells. The plantain family includes all plant parts as mentioned below.

It is particularly preferred according to the invention to treat the plantain plants of those plant species which are in each case available or in use. Plant varieties are understood as meaning plants which have novel properties ("traits") which have been obtained by conventional breeding, by mutagenesis or by recombinant DNA techniques. They may be varieties (varieties), lines (fleeds), biotypes and genotypes.

In a preferred embodiment, the above-mentioned musaceae plants treated according to the invention are barley, cavendish and dwarf cavendish varieties, preferably cavendish varieties.

The treatment method according to the invention can be used for treating Genetically Modified Organisms (GMOs), such as plants or seeds. Genetically modified plants (or transgenic plants) are plants in which a heterologous gene has been stably integrated into the genome. Basically, the term "heterologous gene" refers to a gene provided or assembled outside the plant, which confers new or improved agronomic or other characteristics, after introduction into the nuclear, chloroplast or mitochondrial genome of a transformed plant, by expression of a protein or polypeptide of interest, or by down-regulation or switching off another gene or other gene present in the plant (for example by means of antisense, co-suppression or RNAi [ RNA interference ]). The heterologous gene present in the genome is also referred to as a transgene. A transgene defined by its specific presence in the plant genome is referred to as a transformation event or transgenic event.

Depending on the plant species or plant varieties, their location and their growth conditions (soil, climate, vegetative phase, nutrition), the treatment according to the invention may also produce superadditive ("synergistic") effects. For example, the following effects are possible, which are beyond the actually expected effect: reducing the application rate and/or widening the spectrum of action and/or increasing the efficacy of the active substances and compositions which can be used according to the invention, better plant growth, increasing tolerance to high or low temperatures, increasing tolerance to drought or to water or soil salts, increasing flowering performance, easier harvesting, accelerated ripening, higher yields, larger fruits, higher plant height, deeper leaf greenness, earlier flowering, better quality and/or higher nutritional value of the harvested crops, higher fruit sugar concentration, better storability and/or processability of the harvested crops.

Isotianil also has a strengthening effect on plants at a certain application rate. They are therefore suitable for mobilizing the plant defense system against attack by microorganisms and animal pathogens. This may be one of the reasons for the increased efficacy of the combination according to the invention, for example against fungi. Plant-strengthening (resistance-inducing) substances are also to be understood herein as meaning those substances or substance combinations which are capable of stimulating the defense system of plants in such a way that, when subsequently inoculated with microorganisms and animal pathogens, the treated plants exhibit a large degree of resistance to these microorganisms and animal pathogens. The substances according to the invention can therefore be used for protecting plants against the abovementioned pathogens for a certain period of time after the treatment.

Preferably, the plantago plants and plant varieties treated according to the invention include all plants which contain genetic material which confers particularly advantageous, useful traits to these plants (whether this is achieved by breeding and/or biotechnology).

It is also preferred that the plants and plant varieties of the family Musaceae treated according to the present invention are resistant to one or more biotic stress factors, i.e. that these plants have an improved defense against animal and microbial pathogens, such as nematodes, insects, mites, phytopathogenic fungi, bacteria, viruses and/or viroids. Those which must preferably be mentioned in this context are the Musaceae family which is resistant to phytopathogenic fungi or viruses.

The plantagineae plants and plant species which may also be treated according to the present invention are those plants which are resistant to one or more abiotic stress factors. Abiotic stress conditions can include, for example, drought, low and high temperature conditions, osmotic stress, waterlogging, increased soil salinity, increased mineral exposure, ozone conditions, high light conditions, limited nitrogen nutrient availability, limited phosphorus nutrient availability, or shade avoidance.

The plantaaceae and plant species which may also be treated according to the invention are those in which the vaccine or therapeutic protein is expressed heterologously. They include, for example, hepatitis B antigen.

The plantaaceae and plant species which may also be treated according to the invention are those plants which are characterised by improved yield characteristics. In these plants, increased yield may be caused, for example, by: improved plant physiology, improved plant growth and improved plant development, such as water use efficiency, water retention efficiency, improved nitrogen use, increased carbon assimilation, improved photosynthesis, improved seed vigor and accelerated maturation. Furthermore, yield can be affected by improved plant architecture (under stress and non-stress conditions), including early flowering, control of flowering to produce hybrid seed, seedling vigor, plant size, internode number and distance, root growth, seed size, fruit size, pod or ear number, seed number per pod or ear, seed biomass, increased seed filling, reduced seed abscission, reduced dehiscence of dehiscence horns (pod shatter), and lodging resistance. Other yield-related traits include seed composition, such as carbohydrate content, protein content, oil content and oil composition, nutritional value, reduction in anti-nutritional compounds, improved processability and improved storability.

The plantain plants that can be treated according to the invention are hybrid plants that have expressed heterosis or hybrid vigor, which generally leads to higher yields, higher vigor, healthier plants and higher resistance to biotic and abiotic stress factors. Such plants are typically made by crossing one selfing male sterile parent line (female parent) with another selfing male fertile parent line (male parent). Hybrid seed is typically harvested from male sterile plants and sold to growers. Male sterile plants can sometimes (e.g. in maize) be made by detasseling (i.e. mechanically removing the male reproductive organs or male flowers), but, more commonly, male sterility is caused by genetic determinants in the plant genome. In such cases, and especially when the seed is the desired product to be harvested from the hybrid plant, it is often useful to ensure that the male fertility of the hybrid plant containing the genetic determinant that results in male sterility is fully restored. This can be achieved by ensuring that the male parents have a suitable fertility restorer gene which is capable of restoring male fertility to hybrid plants containing genetic determinants which cause male sterility. Genetic determinants that cause male sterility may be located in the cytoplasm. Examples of Cytoplasmic Male Sterility (CMS) are described, for example, in Brassica species (Brassica species). However, genetic determinants that cause male sterility can also be located in the nuclear genome. Male-sterile plants can also be obtained by plant biotechnology methods such as genetic engineering. A particularly useful method for obtaining male sterile plants is described in WO 89/10396, in which for example a ribonuclease (e.g.Bacillus RNAse) is selectively expressed in tapetum cells in stamens. Fertility can then be restored by expressing a ribonuclease inhibitor (e.g., a barnase inhibitor) in tapetum cells.

The plantain plants or plant species (obtained by plant biotechnology methods such as genetic engineering) which can be treated according to the invention are herbicide-tolerant plants, i.e. plants which are tolerant to one or more given herbicides. These plants can be obtained by genetic transformation or by selecting plants containing mutations conferring tolerance to said herbicides.

Herbicide tolerant plants are, for example, glyphosate tolerant plants, i.e. plants which are tolerant to the herbicide glyphosate or salts thereof. For example, glyphosate tolerant plants may be obtained by transforming plants with a gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes are the AroA gene (mutant CT7) of the bacterium salmonella typhimurium (salmonella sp.), the CP4 gene of Agrobacterium (Agrobacterium sp.), the gene encoding Petunia (Petunia) EPSPS, the gene encoding tomato EPSPS or the gene encoding Eleusine (Eleusine) EPSPS. It may also be a mutated EPSPS. Glyphosate tolerant plants may also be obtained by expressing a gene encoding a glyphosate oxidoreductase. Glyphosate-tolerant plants may also be obtained by expressing a gene encoding a glyphosate acetyltransferase. Glyphosate tolerant plants may also be obtained by selecting plants containing naturally occurring mutations in the above genes.

Other herbicide-resistant plants are, for example, plants which are tolerant to herbicides which inhibit glutamine synthetase, such as bialaphos (bialaphos), glufosinate (phosphinothricin) or glufosinate (glufosinate). The plants can be obtained by expressing enzymes which detoxify the herbicide or by expressing glutamine synthetase mutants which are resistant to inhibition. One such potent detoxification enzyme is, for example, an enzyme encoding glufosinate acetyltransferase (e.g., the bar or pat protein of a species of the genus Streptomyces). Plants expressing exogenous glufosinate acetyltransferase have been described.

Other herbicide tolerant plants are also plants which are tolerant to herbicides which inhibit hydroxyphenylpyruvate dioxygenase (HPPD). Hydroxyphenylpyruvate dioxygenase is an enzyme which catalyzes the reaction which converts p-Hydroxyphenylpyruvate (HPP) to homogentisate. Plants tolerant to HPPD inhibitors may be transformed with a gene encoding a naturally occurring resistant HPPD enzyme or a gene encoding a mutated HPPD enzyme. Tolerance to HPPD inhibitors can also be obtained by transforming plants with genes encoding certain enzymes that are capable of forming uronigrates even in the presence of HPPD inhibitor inhibition of the native HPPD enzyme. The tolerance of a plant to HPPD inhibitors can also be improved by transforming the plant with a gene encoding a prephenate dehydrogenase in addition to the gene encoding an HPPD-tolerant enzyme.

Other herbicide resistant plants are those resistant to acetolactate synthase (ALS) inhibitors. Known ALS inhibitors include, for example, sulfonylurea, imidazolinone, triazolopyrimidines, pyrimidinyloxy (thio) benzoates and/or sulfonylaminocarbonyltriazolinone herbicides. It is known that different mutations of the ALS enzyme, also known as acetohydroxyacid synthase (AHAS), confer tolerance to different herbicides and herbicide groups. The production of sulfonylurea-tolerant plants and imidazolinone-tolerant plants is described in international publication WO 1996/033270. Other sulfonylurea-and imidazolinone-tolerant plants are also described, for example, in WO 2007/024782.

Other imidazolinone and/or sulfonylurea tolerant plants can be obtained by mutagenesis, selection in cell culture in the presence of herbicides or by mutation breeding.

The plantaaceae plants or plant varieties (obtained by plant biotechnology methods such as genetic engineering) which may also be treated according to the invention are insect-resistant transgenic plants, i.e. plants which are resistant to attack by certain target insects. Such plants may be obtained by genetic transformation or by selection of plants containing mutations conferring resistance to said insects.

As used herein, "insect-resistant transgenic plant" includes any plant containing at least one transgene comprising a coding sequence encoding the following proteins:

1) an insecticidal crystal protein or insecticidal portion thereof from Bacillus thuringiensis (Bacillus thuringiensis), such as the insecticidal crystal proteins described in the following website:httn：//www.lifesci.sussex.ac.uk/Home/Neil Crickmore/Bt/or an insecticidal portion thereof, e.g., a Cry protein of the class Cry1Ab, Cry1Ac, Cry1F, Cry2Ab, Cry3Ae, or Cry3Bb, or an insecticidal portion thereof; or

2) A crystal protein or portion thereof from bacillus thuringiensis having pesticidal activity in the presence of a second crystal protein or portion thereof from bacillus thuringiensis, for example a binary toxin consisting of Cy34 and Cy35 crystal proteins; or

3) A hybrid insecticidal protein comprising portions of two different insecticidal crystal proteins from bacillus thuringiensis, e.g., a mixture of proteins of 1) above or a mixture of proteins of 2) above, e.g., cry1a.105 protein produced by corn event MON98034 (WO 2007/027777); or

4) The protein of any one of the above points 1) to 3), wherein some, in particular 1 to 10 amino acids are replaced by another amino acid, thereby obtaining a higher insecticidal activity against the target insect species and/or expanding the range of the target insect species affected and/or due to introducing changes into the encoding DNA during cloning or transformation, such as the Cry3Bb1 protein in corn event MON863 or MON88017, or the Cry3A protein in corn event MIR 604;

5) an insecticidal secreted protein or insecticidal portion thereof from Bacillus thuringiensis or Bacillus cereus, such as a Vegetative Insecticidal Protein (VIP) as listed in the following website: http: html, e.g. proteins from the VIP3Aa protein class; or

6) A secreted protein from bacillus thuringiensis or bacillus cereus having pesticidal activity in the presence of a second secreted protein from bacillus thuringiensis or bacillus cereus, such as a binary toxin consisting of a VIP1A and a VIP2A protein; or

7) A mixture of pesticidal proteins comprising portions of different secreted proteins from bacillus thuringiensis or bacillus cereus, such as a mixture of proteins of 1) or a mixture of proteins of 2) above; or

8) The protein of any of the above points 1) to 3), wherein some, in particular 1 to 10 amino acids are substituted by another amino acid, thereby obtaining a higher insecticidal activity against the target insect species and/or expanding the range of the target insect species affected and/or due to introducing changes into the encoding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in cotton event COT 102.

Of course, insect-resistant transgenic plants as used herein also include any plant comprising a combination of genes encoding proteins of any of the above categories 1 to 8. In one embodiment, the insect-resistant plant contains more than one transgene encoding a protein of any of classes 1 to 8 above, to expand the range of target insect species affected, or to delay the development of resistance by the insect to the plant by using different proteins that have insecticidal activity against the same target insect species but differ in mode of action (e.g., bind to different receptor binding sites of the insect).

The Musaceae plant or plant variety (obtained by plant biotechnology methods such as genetic engineering) treated according to the present invention may also be tolerant to abiotic stress factors. Such plants may be obtained by genetic transformation or by selecting plants containing mutations conferring said stress resistance. Particularly useful stress tolerant plants include:

a. a plant comprising a transgene capable of reducing the expression and/or activity of a poly (ADP-ribose) polymerase (PARP) gene in a plant cell or plant;

b. a plant comprising a stress tolerance-enhanced transgene capable of reducing the expression and/or activity of a PARP-encoding gene in a plant or plant cell;

c. a plant comprising a transgene with enhanced stress tolerance encoding a plant functional enzyme of the nicotinamide adenine dinucleotide salvage biosynthetic pathway, said plant functional enzyme comprising nicotinamide enzyme, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase, nicotinamide adenine dinucleotide synthetase or nicotinamide phosphoribosyltransferase.

Administration forms

According to the invention, the treatment of plantago plants and plant parts and propagation material with isotianil is carried out directly or by treating their environment, habitat or storage space by conventional treatment methods, for example by drip irrigation, spraying, atomizing, spraying, broadcasting, painting on, injection.

In a particularly preferred embodiment of the invention, isotianil or a formulation thereof is used for application for treating vegetative propagation material, or for rhizome or foliar application, or drip application, particularly preferably drip application, preferably once every 30 days, more preferably every 14 days, preferably using from 2.5 to 0.5g fosetyl-aluminum/plant and from 0.035 to 0.015g IST/plant, more preferably from 2.0 to 1.0g fosetyl-aluminum/plant and from 0.03 to 0.02g IST/plant, even more preferably from 2.0 to 1.0g fosetyl-aluminum/plant and from 0.03 to 0.02g IST/plant, most preferably 1.6g fosetyl-aluminum/plant and 0.024g IST/plant.

In another particularly preferred embodiment of the invention, isotianil or a formulation thereof is used for the treatment of vegetative propagation material, or for rhizome or foliar application, or for dip application, particularly preferably drip application, preferably once every 30 days, more preferably every 14 days, preferably using 0.45 to 0.1g fosetyl-aluminum/plant and 0.04 to 0.015g IST/plant, more preferably 0.4 to 0.15g fosetyl-aluminum/plant and 0.04 to 0.02g IST/plant, even more preferably 0.35 to 0.25g fosetyl-aluminum/plant and 0.035 to 0.025g IST/plant and most preferably 0.28g fosetyl-aluminum/plant and 0.028g IST/plant.

In an alternative embodiment of the invention, isotianil or a formulation thereof is applied in granular (for fosetyl-aluminum) form for the treatment of soil.

In another particularly preferred embodiment of the invention, isotianil or a formulation thereof as active ingredient only is used for treating vegetative propagation material, or for rhizome or foliar application, or drip irrigation application, particularly preferably drip irrigation application, preferably once every 30 days, more preferably every 14 days, preferably 0.035 to 0.015g IST per plant, more preferably 0.03 to 0.02g IST per plant, even more preferably 0.03 to 0.02g IST per plant and most preferably 0.024g IST per plant.

In another particularly preferred embodiment of the invention, isotianil or a formulation thereof as active ingredient only is used for treating vegetative propagation material, or for rhizome or foliar application, or drip irrigation application, particularly preferably drip irrigation application, preferably once every 30 days, more preferably every 14 days, preferably 0.04 to 0.015g IST per plant, more preferably 0.04 to 0.02g IST per plant, even more preferably 0.035 to 0.025g IST per plant and most preferably 0.028g IST per plant.

In a preferred embodiment, the first treatment is carried out 15 days before planting, regardless of the subsequent treatment interval.

Isotianil can be converted into conventional formulations, such as solutions, emulsions, suspensions, powders, foams, pastes, granules, sachets, aerosols, microcapsules in polymers, and ULV cold and hot fogging formulations, according to their respective physical and/or chemical properties.

These formulations are prepared in a known manner, for example by mixing isotianil with extenders, i.e. liquid solvents, liquefied gases under pressure and/or solid carriers, optionally with the use of surfactants, i.e. emulsifiers and/or dispersants and/or foaming agents. If water is used as extender, it is also possible, for example, to use organic solvents as cosolvents. Suitable liquid solvents are mainly: aromatic compounds such as xylene, toluene or alkylnaphthalene; chlorinated aromatic compounds or chlorinated aliphatic hydrocarbons, such as chlorobenzene, vinyl chloride or dichloromethane; aliphatic hydrocarbons, such as cyclohexane or paraffins, for example mineral oil fractions; alcohols, such as butanol or glycols, and ethers and esters thereof; ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone; strongly polar solvents such as dimethylformamide and dimethylsulfoxide, and water; and mineral, animal and vegetable oils, such as palm oil or other plant seed oils. Liquefied gaseous extenders or carriers are to be understood as meaning those liquids which are gaseous at normal temperature and pressure, for example aerosol propellants such as halogenated hydrocarbons and butane, propane, nitrogen and carbon dioxide. Suitable solid carriers are: for example ground natural minerals such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or diatomaceous earth; and ground synthetic minerals such as highly dispersible silica, alumina and silicates. Suitable solid carriers for granules are: for example crushed and fractionated natural rocks such as calcite, pumice, marble, sepiolite, dolomite; and synthetic particles of inorganic and organic powders, as well as particles of organic materials such as sawdust, coconut shells, corn cobs, and tobacco stalks. Suitable emulsifiers and/or foaming agents are: for example nonionic, cationic and anionic emulsifiers, such as polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers (e.g. alkylaryl polyglycol ethers), alkylsulfonates, alkyl sulfates, arylsulfonates and protein hydrolysates. Suitable dispersants are: such as lignosulfite waste liquors and methylcellulose.

Adhesives such as carboxymethylcellulose, natural and synthetic polymers in powder, granule or latex form (e.g., gum arabic, polyvinyl alcohol, polyvinyl acetate) and natural phospholipids (e.g., cephalins and lecithins), as well as synthetic phospholipids, can be used in the formulations. Other additives may be mineral and vegetable oils.

Colorants such as inorganic pigments, e.g., iron oxide, titanium oxide, prussian blue; and organic dyes, such as alizarin, azo, and metal phthalocyanine dyes; and micronutrients such as salts of iron, manganese, boron, copper, cobalt, molybdenum and zinc.

Typically, the formulation contains 5 to 95% by weight active, preferably 10 to 70% by weight active, more preferably 15 to 30% by weight active, and most preferably 20% by weight active.

The control of microorganisms and animal pathogens by treating vegetative propagation material of plants has been known for a long time and is the subject of continuous improvement. However, the handling of vegetative propagation material still involves a series of problems which cannot be solved in a satisfactory manner. It is therefore desirable to develop a method for protecting vegetative propagation material and germinating plants which does not require, or at least significantly reduces, the additional application of plant protective products after planting or after the emergence of the plants. Furthermore, it is desirable to optimize the amount of active substance used in order to provide the asexual propagation material and the germinating plant with the best possible protection against microbial pathogens without causing damage to the plant itself by the active substance used. In particular, the method of treating vegetative propagation material should also take into account the inherent properties of the transgenic plants in order to achieve optimum protection of the vegetative propagation material and of the germinating plants, while at the same time keeping the application rate of the plant protective products as low as possible.

The invention therefore also relates in particular to a method for protecting vegetatively propagated materials and germinating plants from attack by microorganisms and animal pathogens by treating seeds and vegetatively propagated materials with a composition according to the invention.

The invention also relates to the use of the composition according to the invention for treating vegetatively propagated material to protect the vegetatively propagated material and the germinating plants from attack by microorganisms and animal pathogens.

One of the advantages of the present invention is that, due to the special systemic properties of the compositions of the present invention, the treatment of the vegetative propagation material with these compositions provides protection not only to the vegetative propagation material itself from microbial and animal pathogens, but also to the plants that grow from it after planting. In this way, it may not be necessary to treat the crop immediately at the time of sowing or shortly thereafter.

A further advantage is that the composition of the invention can also be used, in particular, on transgenic vegetative propagation material.

The compositions of the invention are suitable for protecting vegetative propagation material of any plant species used in agriculture, greenhouse, forestry or horticulture. In particular, it is a vegetative propagation material of the family musaceae.

Within the scope of the present invention, the compositions of the invention are applied to the vegetative propagation material alone or in suitable formulations. Preferably, the vegetative propagation material is treated in a sufficiently stable state so that no damage occurs during the treatment. In general, vegetative propagation material can be processed at any time between harvesting and planting. Typically, vegetative propagation material is used which has been isolated from the plant and has had the cob (cob), husk, stem, pod, hair or pulp removed.

Generally, when dealing with vegetative propagation material, care must be taken to select the amount of the composition of the invention and/or other additives applied to the vegetative propagation material so that the germination of the vegetative propagation material is not adversely affected or the plant grown from the vegetative propagation material is not damaged. This must be taken into account in particular in the case of active substances which can have phytotoxic effects at certain application rates.

The composition of the invention can be applied directly, in other words without further components and without dilution. In general, it is preferred to apply the composition in the form of a suitable formulation to the vegetative propagation material. Suitable formulations and methods for treating seeds and vegetative propagation material are known to the person skilled in the art.

The compounds which can be used according to the invention and are selected from the compounds of the formula (I) can be converted into the customary formulations, such as solutions, emulsions, suspensions, powders, foams and ULV formulations

These formulations are prepared in a known manner by mixing a compound selected from the compounds of the formula (I) with the customary additives, such as the customary extenders as well as solvents or diluents, colorants, wetting agents, dispersants, emulsifiers, antifoams, preservatives, secondary thickeners, adhesives, gibberellins, mineral and vegetable oils and water.

Colorants which may be present in the formulations usable according to the invention are all colorants conventionally used for this purpose. In this context, pigments which are sparingly soluble in water and dyes which are soluble in water can be used. Examples which may be mentioned are colorants known under the names rhodamine b (rhodamine b), c.i. pigment red 112 and c.i. solvent red 1.

Wetting agents which may be present in the formulations which can be used according to the invention are all substances which are conventionally used for formulating agrochemical active substances and promote wetting. Alkyl naphthalene sulfonates such as diisopropyl naphthalene sulfonate or diisobutyl naphthalene sulfonate can be preferably used.

Suitable dispersants and/or emulsifiers which may be present in the formulations which can be used according to the invention are all nonionic, anionic and cationic dispersants conventionally used for formulating agrochemical active substances. The following substances can preferably be used: a non-ionic or anionic dispersant, or a mixture of non-ionic or anionic dispersants. Suitable nonionic dispersants which may be mentioned are, in particular, ethylene oxide/propylene oxide block polymers, alkylphenol polyglycol ethers and tristyrylphenol polyglycol ethers, and also their phosphorylated or sulfated derivatives. Suitable anionic dispersants are, in particular, lignosulfonates, polyacrylates and aryl-sulphonate/formaldehyde condensates.

The antifoams which may be present in the formulations which can be used according to the invention are all foam-suppressor substances conventionally used for the formulation of agrochemical active substances. Silicone antifoam agents and magnesium stearate can preferably be used.

Preservatives which may be present in the formulations which can be used according to the invention are all substances which can be used for this purpose in agrochemical compositions. Examples which may be mentioned include dichlorophenol and benzyl alcohol hemiformal.

Secondary thickeners which may be present in the formulations which can be used according to the invention are all substances which can be used for this purpose in agrochemical compositions. Preferably, suitable secondary thickeners are cellulose derivatives, acrylic acid derivatives, xanthan gum, modified clays and highly dispersed silica.

The adhesives which may be present in the formulations which can be used according to the invention are all conventional binders which can be used in mordants. Preference is given to polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose sodium.

Gibberellins which may be present in the formulations which may be used according to the present invention are preferably gibberellin a1, gibberellin A3 (gibberellic acid), gibberellin a4 and gibberellin a 7. Gibberellic acids are particularly preferred.

Gibberellins are known (see R.Wegler "Chemie der Pfalanzenschutz-und

″[Chemistry of plant protection and pesticideagents]Vol.2, Springer Verlag, Berlin-Heidelberg-New York, 1970, p.401-412).

The preparations which can be used according to the invention can be used for the treatment of various types of seed either directly or after prior dilution with water. Thus, the concentrate or the preparation obtainable therefrom by dilution with water can be used for dressing seeds of the family musaceae. The preparations which can be used according to the invention or their diluted preparations can also be used for the treatment of vegetative propagation material of transgenic plants. Here, an additional synergistic effect can be produced in combination with the substance formed by expression.

The application rate of the preparations which can be used according to the invention can vary within wide limits. The application rate depends on the content of the respective active substance in the preparation and on the vegetative propagation material. In general, the application rate of the active substance is preferably from 0.001 to 50g/kg of vegetative propagation material, more preferably from 0.01 to 15g/kg of vegetative propagation material.

Mixture of

The compounds selected from the compounds of formula (I) can be used as such, in the form of formulations or also in the form of mixtures with known fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, safeners, soil improvement products or products for relieving plant stress (e.g. Myconate), for example in order to broaden the spectrum of action or to prevent the development of resistance. In many cases, this results in a synergistic effect, that is to say the efficacy of the mixture exceeds that of the individual components.

In a preferred embodiment, the invention is a mixture of isotianil with at least one other active ingredient selected from the group consisting of fosetyl-aluminum, the mono-and disodium salts of phosphorous acid, the mono-and dipotassium salts of phosphorous acid and the mono-and diammonium salts of phosphorous acid (such as Phostrol), more preferably fosetyl-aluminum; wherein the ratio of isotianil to the mixing partner in% by weight is preferably from 1 to 20 to 1 to 100, more preferably from 1 to 40 to 1 to 80, even more preferably from 1 to 60 to 1 to 75.

In another preferred embodiment, the invention is a mixture of isotianil with at least one other active ingredient selected from the group consisting of fosetyl-aluminum, mono-and disodium phosphites, mono-and dipotassium phosphites, and mono-and diammonium phosphites (such as Phostrol), more preferably fosetyl-aluminum; wherein the ratio of isotianil to the mixing partner in% by weight is preferably from 1 to 50, more preferably from 1 to 3 to 1 to 30, even more preferably from 1 to 5 to 1 to 15.

According to the invention, the term "mixture" means possible different combinations of at least two of the above-mentioned active substances, for example a ready-to-use mixture, a tank mix (which is understood to mean a spray slurry prepared by mixing and diluting a preparation of the individual active substances before application) or a combination thereof (for example, a binary ready-to-use mixture of two of the above-mentioned active substances is made into a tank mix by using a preparation of a third individual substance). According to the invention, it is also possible to use the individual active substances sequentially, i.e. one after the other, at reasonable intervals of hours or days, in the case of seed treatment, for example also by applying a plurality of layers containing different active substances. Preferably, the order in which the individual actives may be used is not critical.

The compounds of the formula (I) can be used as such, in the form of their formulations or in the use forms prepared therefrom, such as ready-to-use solutions, suspensions, wettable powders, pastes, soluble powders, powders and granules. They are applied in the customary manner, for example by spraying, atomizing, scattering, dusting, foaming, painting or the like. Furthermore, the compounds of the formula (I) can be applied by the ultra-low-volume method or the active substance preparation or the active substance itself can be injected into the soil. Vegetative propagation material of plants may also be treated.

When compounds selected from the compounds of formula (I) are used, the application rate can vary within wide limits, depending on the type of application. The application rate of the active substance in the treatment of the plant parts is preferably from 0.1 to 10000g/ha, more preferably from 10 to 1000 g/ha. In the treatment of the vegetative propagation material, the application rate of the active substance is preferably from 0.001 to 50g/kg of vegetative propagation material, more preferably from 0.01 to 10g/kg of vegetative propagation material. In the treatment of soils, the application rate of the active substance is preferably from 0.1 to 10000g/ha, more preferably from 1 to 5000 g/ha.

The following examples are intended to illustrate the invention without imposing any limitation.

Example (b):

example 1

Efficacy of isotianil and aluminum triphosphate against Fusarium oxysporum No. 4 race

This example illustrates the use of a composition containing isotianil against bananas of the cavendish typeFusarium oxysporum No. 4 Seed of small speciesThe efficacy of (1).

The trial was performed using a Random Complete Block Design (RCBD) with 4 treatments (10 plants/treatment) as follows:

a) the raw materials are not treated, and the raw materials are not treated,

b) aliette80WG 2g per plant (FEA 1.6g active ingredient per plant),

c) isotianil SC200 g/L0.12 mL/plant (IST 0.024g active ingredient/plant), and

d) aliette80WG + Isotianil SC200-2g +0.12 mL/plant (1.6+0.024g active ingredient/plant).

The application was carried out monthly with soil saturation.

Results

Fusarium infection is affected by the application of different fungicides

Test 1: preliminary test results show that 90% protection against fusarium infection can be provided monthly by preventive application of 2 grams of Aliette +0.12mL of isotianil per plant in a soil drenching manner. Infection was controlled at 30% and 50% when 0.12mL isotianil and 2 grams of Aliette per plant were applied alone. In this case, the synergy between the two compounds can be determined (Colby formula-efficacy calculated by Abbott: 65%). Furthermore, symptom development was delayed on the treated plants by 22 days (Aliette and isotianil alone) and 155 days (Aliette + isotianil) compared to the untreated control group (UTC).

Example 2

Isotianil and Isotianil + fosetyl-aluminum efficacy against Fusarium oxysporum number 1 race

This example illustrates that compositions comprising isotianil are effective against the banana variety MicheliaFusarium oxysporum No. 1 Seed of small speciesThe efficacy of (1).

The trial was performed using a Random Complete Block Design (RCBD) with 3 blocks, each block repeated 8 times-all treatments were as follows:

e) the raw materials are not treated, and the raw materials are not treated,

f) aliette80WG 2g per plant (FEA 1.6g active ingredient per plant),

g) isotianil SC200 g/L0.12 mL/plant (IST 0.024g active ingredient/plant). And

h) aliette80WG + isotianil SC200-2g +0.12 mL/plant (1.8+0.024g active ingredient/plant).

Application was carried out in the following manner: soil saturation was started in the nursery 15 days before planting and continued for the time of planting and then once a month (application at 40cm base and periphery of the plant). The evaluation tests were the percentage of infected plants and the severity of the trunk infection at the end of the test period (severity scale 0 ═ asymptomatic to 4 ═ plant death).

The results of a trial at costa rica in 2015/2016

Test 2: the final test results show that prophylactic application of 2 grams of Aliette +0.12mL of isotianil per plant once a month in a soil-drenched manner provides 100% protection from fusarium infection 90 days after planting. Infection was controlled at 43% and 27% when 0.12mL isotianil and 2 grams of Aliette per plant were applied alone. In this case, the synergy between the two compounds can be determined (Colby formula-efficacy calculated by Abbott: 58%). Furthermore, despite the lower persistence of the individual compounds, the mixtures showed a higher level of control 180 days after planting and reduced the severity of infection at the culmination.

Example 3

Room temperature test-efficacy of Isotianil + fosetyl-aluminum against Fusarium oxysporum No. 4 race

This example illustrates the use of a composition containing isotianil against the banana plant, the variety GrandenNaineFusarium oxysporum No. 4 microspeciesThe efficacy of (1).

The experiment was carried out in a greenhouse with 30 plants treated each time (10 plants were replicated 3 times each). Two months old banana plants were transplanted into the infected soil. Compounds were used for saturation 6 days prior to transplantation (protective application). A second administration was performed 4 weeks after the first administration. Application was carried out in the following manner:

j) aliette80WG 2g per plant (FEA 1.6g active ingredient per plant), and

k) aliette80WG + isotianil SC200-2g +0.12mL per plant (1.8+0.024g active ingredient per plant), compared to i) untreated contaminated plants.

Six weeks after inoculation, the plants were scored according to the isometric scale of discoloration (typical symptoms of fusarium oxysporum). Grade 0 ═ asymptomatic to 6 ═ total plant necrosis

Results

Test 3: the final test results at 6 weeks post inoculation showed a significant difference in protective protection as follows:

i) untreated contaminated (average of 3 replicates-6),

j) aliette 2g per plant (average of T1C13 replicates ═ 5.3), and

k) aliette + isotianil 2g +0.12 mL/plant (Trt T4C13 replicates with an average score of 1.7). When Aliette is less effective, the fosetyl-al + isotianil mixture provides good protection against Foc under these very severe infection conditions, indicating that it is advantageous to mix it with isotianil.

Example 4-soil application-2 ratio of test applications

Isotianil and fosetyl-aluminium (ratio 60: 1 and ratio 10: 1) applied in a drench against fusarium oxysporum Efficacy of fungus No. 4 microspecies

This example illustrates the two compositions comprising fosetyl-aluminum and isotianil applied in a drenched manner against banana plantsFusarium oxysporum No. 4 microspeciesThe efficacy of (1).

The test was carried out in plantations of a Randomized Complete Block Design (RCBD) of the cavendish variety (3 replicates/treatments-a total of 20 plants/plot evaluated), comprising untreated, Fosetyl-aluminum + isotianil (SP102000028595WG 77% -ratio 10: 1) -0.4 g/plant (0.3+0.03g active ingredient/plant), Fosetyl-aluminum (Fosetyl) + isotianil (SP102000033663 WG 76.5% -ratio 60: 1) -0.4 g/plant (0.3+0.005 g/plant). The ratio of 2 formulations was calculated to produce a similar amount of fosetyl-aluminum and a variable amount of isotianil. All FEA + IST applications were performed as drench applications, once a month with 500ml water per plant.

Preliminary test results at site 2 showed that the use of FEA + IST in the drench application method significantly delayed the development of Fusarium oxysporum # 4 race, both at a 10: 1 and 60: 1 ratio. This delay in disease development translates into a retention of production potential in the treated cells.

Results of two experiments:

example 5 foliar application-test of 2 ratios

Isotianil and fosetyl-aluminium (ratio 60: 1 and ratio 10: 1) applied as foliar spray against fusarium oxysporum Efficacy of fungus No. 4 microspecies

This example illustrates two compositions containing fosetyl-aluminum and isotianil applied as foliar sprays to combat bananas in plantsFusarium oxysporum No. 4 microspeciesThe efficacy of (1).

The trial was carried out in plantations of a Randomized Complete Block Design (RCBD) of the cavendish variety (3 replicates/treatments-a total of 20 plants/plot evaluated), comprising untreated, Fosetyl-aluminum + isotianil (SP102000028595WG 77% -ratio 10: 1) -2 g/plant (1.4+0.14g active ingredient/plant), Fosetyl-aluminum (Fosetyl) + isotianil (SP102000033663 WG 76.5% -ratio 60: 1) -2 g/plant (1.5+0.025 g/plant). All FEA + IST applications were carried out as foliar applications, once a month with 50ml of water per plant.

Preliminary test results at site 2 showed that the use of FEA + IST as a foliar spray significantly delayed the development of Fusarium oxysporum # 4 race, both at a 10: 1 and 60: 1 ratio. This delay in disease development means a reservation of productive potential in the treated cells.

Results of two experiments:

conclusions regarding the protection of banana plantations from fusarium oxysporum causing wilt

The five examples recorded demonstrate that it is advantageous to use isotianil-based compounds to limit the development of fusarium oxysporum sp.4 in banana plants. The mixture of isotianil and fosetyl-aluminum showed even better protection in field plantations. The 2 ratios tested in drench application and foliar spray showed a significant reduction in disease infection and in some conditions would delay the development of barnacle disease in banana plantations. For the producer, this advantage means better plantation survival, with reduced infected plants and increased yield.

Claims (17)

1. Use of isotianil (formula (I)) for controlling panama disease in plants of the Musaceae family

2. Use of isotianil according to claim 1 for controlling fungi of the genus fusarium in plantaaceae.

3. Use of isotianil according to claim 1 or 2 for controlling fusarium oxysporum cubeba obligate type fungi in plantaaceae.

4. Use of isotianil according to any one of the preceding claims for controlling fungi of fusarium oxysporum cubeba specialized type 1 race in plantaaceae plants.

5. Use of isotianil according to any one of the preceding claims for controlling the fungus fusarium oxysporum cubeba obligate type 4 race in plantaaceae plants.

6. Use of isotianil according to any preceding claim characterized in that the plants of the family musaceae are selected from the cabnadish or horech varieties.

7. Use of isotianil according to any preceding claim, characterized in that isotianil is used in combination with fosetyl-aluminum.

8. Use of isotianil according to any preceding claim, characterized in that isotianil is used in combination with fosetyl-aluminum in a proportion of from 1 to 60 to 1 to 75, in% by weight.

9. Use of isotianil according to any preceding claim, characterized in that isotianil is used in combination with fosetyl-aluminum in a proportion of from 1 to 5 to 1 to 15, in% by weight.

10. Method for controlling panama disease in plantago plants, characterized in that isotianil (I) is used for treating plantago plants.

11. The method according to claim 10, characterized in that isotianil (I) is used in combination with fosetyl-aluminum for the treatment of plantago plants.

12. The method according to claims 10 and 11, characterized in that the first treatment is carried out 15 days before planting.

13. Method according to claims 10 and 11, characterized in that the treatment is carried out at intervals of 30 days, preferably at intervals of 14 days.

14. Method according to claims 10 to 13, characterized in that the amount of active ingredient per treatment per plant is between 2.5 and 0.5g of fosetyl-aluminium and between 0.035 and 0.015g of isotianil, or a combination of both.

15. The method according to claims 10 to 13, characterized in that the amount of active ingredient per treatment per plant is between 0.45 and 0.0.1g of fosetyl-aluminium and between 0.04 and 0.015g of isotianil, or a combination of both.

16. The method according to claim 11, characterized in that the ratio of isotianil to fosetyl-aluminum is from 1 to 60 to 1 to 75 in weight%.

17. The method of claims 10 to 16, wherein the application is drip irrigation application.