BR112020005311A2 - use of isothianil against panama disease - Google Patents

Abstract

The present invention relates to the use of Isothianyl (formula (I)) (I) to control Panama disease in plants of the Musaceae family. In addition, the present invention relates to a method of controlling Panama's disease in plants of the Musaceae family by treating them with Isothianyl (I).

Description

“USE OF ISOTIANILA AGAINST PANAMA DISEASE”

[0001] The present invention relates to the use of Isothianyl (IST) (formula (I)) Cl Cl

N H

S N CN (I) to control Panama disease in plants of the Musaceae family.

[0002] In addition, the present invention relates to a method for controlling Panama disease in plants of the Musaceae family by treating it with Isothianyl or formulations containing Isothianyl (formula (I)).

[0003] Furthermore, the present invention relates to mixtures of Isothianil with at least one additionally active ingredient selected from fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, protective agents, host deficiency inducers, products for improving soil or products to reduce stress on plants, for example, Miconato, in order to broaden the spectrum of action or prevent the development of resistance, for example, for the treatment of Panama disease.

[0004] In a preferred embodiment, the present invention relates to mixtures of Isothianyl with at least one additionally active ingredient selected from Fosetil-Al, and mono- and dibasic sodium, potassium and ammonium phosphites (for example, Phostrol) to control Panama disease in plants of the Musaceae family.

[0005] The compound of formula (I) is known from WO 99/024 413, WO 2006/098128, JP 2007-84566 and WO 96/29871, inter alia. Invention

[0006] Panama disease is an aggressive plant disease of banana roots caused by the fungal pathogen Fusarium oxysporum, in particular Fusarium oxysporum f.sp.cubense (Foc), race 1 and race 4. It is the most common disease destructive effect on bananas. For example, during the 1950s, Panama's disease wiped out most of the Gros Michel banana production, which, at that time, led the banana variety. Nowadays, new strains of Panama disease are once again threatening the most popular crop, the Cavendish type, today.

[0007] The Fusarium fungus enters the plant's roots and spreads through the plant's xylem vessels. The fungus disrupts the plant's vascular system, which causes the leaves to turn yellow and wither, and the plant dies sooner or later.

[0008] The pathogen affects banana crops worldwide with so far 4 breeds. His "Race 1" has spread to the Philippines and Indonesia, where the even more aggressive "Tropical Race 4" is now spreading. Race 1 is also on the rise in Africa and Australia. It has not yet arrived in Latin America, but it is only a matter of time before Panama's disease is also devastating on banana plantations in that region. This causes huge financial losses and jeopardizes the existence of related banana producers.

[0009] Until now, the Fusarium pathogen is resistant to fungicides and there is no chemical solution available.

[0010] In WO 2010/037482, Isothianyl derivatives are described to control microbial and animal pathogens in plants of the Musaceae family, only exemplified by Sigatoka-negra (Mycosphaerella fijiensis).

[0011] It has now been found that Isothianil and mixtures of Isothianil with at least one additionally active ingredient are particularly suitable for the control of Panama disease in plants of the Musaceae family.

[0012] A first subject of the invention therefore consists of using Isothianyl to control Panama disease in plants of the Musaceae family.

[0013] An additional matter of the invention therefore consists in the use of Isothianil to control Panama disease in plants of the genus Musa.

[0014] An additional matter of the invention consists of a method of controlling Panama disease in plants of the Musaceae family, characterized by the fact that the plants of the Musaceae family are treated with Isothianyl.

[0015] Another subject of the present invention consists of the use and methods mentioned above, in which Isothianil is used in combination with at least one additionally active ingredient selected from fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, agents protection agents, host deficiency inducers, soil improvement products or products to reduce stress in plants, for example, Miconato, in order to broaden the spectrum of action or prevent the development of resistance, for example ,.

[0016] A preferred embodiment of the present invention consists of the use and methods mentioned above, in which race 1 or race 4 of Fusarium oxysporum f.sp.cubense causes Panama disease.

[0017] Another preferred embodiment of the present invention is the use and methods mentioned above, in which Isothianyl or Isotanil in a combination with at least one additionally active ingredient is applied, wherein the additional ai is preferably selected from of Fosetil-Al and mono- and dibasic sodium, potassium and ammonium phosphites and, more preferably, Fosetil-Al.

[0018] Another additional preferred embodiment of the present invention consists of the use and methods mentioned above, in which the method of use is by drip application, preferably every 30 days, more preferably, every 14 days, with 2.5 to 0.5 g of Fosetil-Al / plant and 0.035 to 0.015 g of IST / plant, more preferably with 2.0 to 1.0 g of Fosetil-Al / plant and 0.03 to 0, 02 g of IST / plant, even more preferably, with 2.0 to 1.0 g of Fosetil-Al / plant and 0.03 to 0.02 g of IST / plant and, most preferably, with 1.6 g of Fosetil-Al / plant and 0.024 g of IST / plant.

[0019] Another additional preferred embodiment of the present invention is the use and methods mentioned above, in which Isothianyl is used in combination with Fosetil-Al in a weight% ratio of 1 to 60 to 1 to 75.

[0020] Another additional preferred embodiment of the present invention is the use and methods mentioned above, in which the method of use is by drip application, preferably every 30 days, more preferably every 14 days, with 0.45 to 0.1 g of Fosetil-Al / plant and 0.04 to 0.015 g of IST / plant, more preferably, with 0.4 to 0.15 g of

Fosetyl-Al / plant and 0.04 to 0.02 g of IST / plant, even more preferably, with 0.35 to 0.25 g of Fosetil-Al / plant and 0.035 to 0.025 g of IST / plant and, most preferably, with 0.28 g of Fosetil-Al / plant and 0.028 g of IST / plant.

[0021] Another additional preferred embodiment of the present invention is the use and methods mentioned above, in which Isothianyl is used in combination with Fosetil-Al in a weight% ratio of 1 to 5 to 1 to 15.

[0022] Another additional preferred embodiment of the present invention is the use and methods mentioned above, in which the method of use is by drip application, preferably every 30 days, more preferably, every 14 days, with 0.035 to 0.015 g IST / plant, more preferably, with 0.03 to 0.02 g IST / plant, even more preferably, 0.03 to 0.02 g IST / plant and, most preferably , with 0.024 g IST / plant.

[0023] Another additional preferred embodiment of the present invention is the use and methods mentioned above, in which the method of use is by drip application, preferably every 30 days, more preferably, every 14 days, with 0.04 to 0.015 g IST / plant, more preferably, with 0.04 to 0.02 g IST / plant, even more preferably, 0.035 to 0.025 g IST / plant and, most preferably, with 0.028 g IST / plant. Definitions

[0024] The Musaceae family consists, inter alia, of the following species: Musa acuminata, Musa balbisiana, Musa acuminata Colla with the varieties "Dwarf Cavendish", "Giant Cavendish" and "Gros Michel", Musa cavendishii Lamb. ex Paxt., Musa malaccensis Ridl., Musa angcorensis Gagnep., Musa aurantiaca, Musa balbisiana, Musa seminifera Lour., Musa banksii F. Muell., Musjo basjoo, Musa cheesmanii, Musa flaviflora Simmonds, Musa griersonii, Musa itinerans, later , Musa mannii, Musa nagensium, Musa ochracea, Musa ornata Roxb., Musa siamea, Musa sikkimensis, Musa thomsonii Noltie, Musa velutina Wendl. & Drude, Musa alinsanaya, Musa beccarii, Musa boman, Musa borneënsis, Musa bukensis, Musa campestris, Musa coccinea Andrews, Musa uranoscopos Lour, Musa exotica Valmayor, Musa fitzalanii, Musa flavida, Musa gracilata, Musa hirta Beccular. , Musa jackeyi, Musa johnsii, Musa lawitiensis, Musa lolodensis, Musa maclayi, Musa monticola, Musa muluensis, Musa paracoccinea, Musa peekelii, Musa pigmaea Hotta, Musa rubra, Musa salaccensis, Musa splendida A. Chev., Musa surati, Musa surati : Abaca, Japanese banana or banana fiber, Musa troglodytarum, Musa tuberculata, Musa violascens, Musa ingens, Musa paradisiaca sapientm, Musa paradisiaca normali, and crosses of these species.

[0025] Examples of Fusarium fungi that cause Panama disease in plants of the Musaceae family are Fusarium spp, for example, Fusarium pallidoroseum, Fusarium solani anamorph Nectria haematococca, Fusarium oxysporum, Fusarium monomiform teleomorph: Gibberella fujikuroi, Fusarium oxysporum f. sp. cubense (Foc), in particular, race 1 and 4 of Foc, more particularly, race 4 of Foc.

[0026] According to the present invention, Isothianyl or mixtures of Isothianil as described above are particularly useful in combating fungi of the genus Fusarium that cause Panama disease in plants of the Musaceae family, which are Fusarium spp, for example, Fusarium pallidoroseum,

Fusarium solani, Nectria haematococca anamorph, Fusarium oxysporum, Fusarium monomiform teleomorph: Gibberella fujikuroi, Fusarium oxysporum f. sp. Cuban, in particular, race 1 and 4 of Foc, more particularly, race 4 of Foc.

[0027] Isothianyl may, if appropriate, be present in the form of mixtures of various isomeric forms which are possible, in particular, stereoisomers, such as optical isomers.

[0028] Isothianyl can therefore be used to protect plants against attack or to delay attack / symptoms by the pathogens mentioned above within a certain post-treatment period. The period within which protection is granted generally extends from 1 to 30 days, preferably 1 to 14 days, after the plants have been treated with the active substances. Depending on the form of application, the accessibility of the active substances to the plant can be controlled in a targeted manner.

[0029] The good tolerance of plants to Isothianil in the concentrations necessary for the control of plant diseases according to the present invention allows a treatment of aerial and underground parts of plants, of vegetative propagation material and of the soil.

[0030] According to the invention, all plants of the Musaceae family can be treated. The plants of the Musaceae family are, in the present context, understood in such a way as to mean that all plant parts and plant populations, such as wild plants or desired or unwanted crop plants (including naturally occurring crop plants). Cultivation plants can be plants of the Musaceae family that can be obtained by traditional optimization and reproduction methods or, also, by biotechnological and recombinant methods, or combinations of these methods, including transgenic plants of the Musaceae family and including plant varieties capable whether or not they are protected by the Rights of Plant Breeders, such as Gros Michel, Cavendish, Dwarf Cavendish, Dwarf Chinese, Enano, Caturra, Giant Cavendish, Gran Enano, Grande Naine, Williams Hybrid, Valery, Robusta, Poyo, Lacatan, Pisang masak hijau, Monte cristo, Bout rond. Plant parts are meant to mean that all aerial and underground parts and plant organs, such as grass, pseudostem, bud, leaf, bract, leaf sheaths, petiole, blade, flower and root, examples that can be mentioned being leaves , needles, stems, stems, flowers, fruiting bodies, fruit, banana bunch, bunches and seeds, and also roots, tubers, rhizomes, branching, bud roots, secondary growth. Plant parts also include cultivating material and material for generative or vegetative propagation, for example, cuttings, tubers, rhizomes, seedlings and seeds.

[0031] As already mentioned above, all plants of the Musaceae family can be treated according to the invention. In a preferred embodiment, the plant species and plant varieties, and their parts, that are found in the wild or that are obtained by conventional biological reproduction methods, such as hybridization, meristematic cultures, micropropagation, somatic embryogenesis, direct organogenesis or fusion proroplasts are treated. In an additional preferential modality, transgenic plants of the Musaceae family and plant varieties of the Musaceae family that were obtained by recombinant methods, if appropriate in combination with traditional methods (genetically modified organisms), are treated, such as, for example, transformation through of Agrobacterium or bombardment of embryogenic cell particles and micropropagation. The plants of the Musaceae family include all plant parts as mentioned above.

[0032] It is especially preferable to treat, according to the invention, plants of the Musaceae family of those plant varieties that are, in each case, commercially available or are in use. Plant varieties should be understood as plants with innovative properties ("traits") that have been obtained by conventional breeding, mutagenesis or by recombinant DNA techniques. They can be varieties, strains, biotypes or genotypes.

[0033] In a preferred embodiment, the plants of the Musaceae family treated in accordance with the present invention as described above are varieties of Gros Michel, Cavendish and Dwarf Cavendish, preferably of Cavendish.

[0034] The treatment methods according to the invention can be used for the treatment of genetically modified organisms (GMOs), for example, plants or seeds. Genetically modified plants (or transgenic plants) are plants in which a heterologous gene has been stably integrated into the genome. Essentially, the term "heterologous gene" refers to a gene that is supplied or assembled outside the plant and which, by introducing it into the nuclear genome, the chloroplast genome or the transformed plant's mitochondrial genome, confers improved or innovative agronomic properties or other properties by the expression of a protein or polypeptide of interest, or by down-regulation or switching of another gene, or other genes, present in the plant (for example, by means of antisense technology, co-suppression technology or RNAi technology [interference from RNA]). A heterologous gene that is present in the genome is also referred to as a transgene. A transgene that is defined by its specific presence in the plant genome is called a transformation or transgenic event.

[0035] Depending on the species of the plant or the varieties of the plant, its conditions of location and growth (soils, climate, period of vegetation, nutrition), the treatment according to the invention can also result in superadditive effects ("synergistic" ). For example, the following effects are possible, which extend beyond the effects that should currently be expected: reduced application rates and / or broader spectrum of action and / or an increased effectiveness of the active substances and compositions that can be used accordingly. with the invention, better plant growth, increased tolerance for high or low temperatures, increased tolerance for drought or for salinity of water or soil, increased flowering performance, easier harvesting, accelerated ripening, larger harvest fields, larger fruits, height larger plant, greener leaf color, easier flowering, superior quality and / or a higher nutritional value of cultivated crops, higher concentration of sugar in fruits, better storage capacity and / or processability of cultivated crops.

[0036] At certain application rates, Isothianyl can also have a strengthening effect on plants.

Therefore, they are also suitable for mobilizing the plant's defense system against attacks by microbial pathogens and animals. This may be one of the reasons for the increased effectiveness of the combinations according to the invention, for example, against fungi. Plant strengthening substances (resistance induction) in the present context should also be understood as meaning those substances or combinations of substances that are capable of stimulating the plant's defense system so that treated plants, when subsequently inoculated with microbial pathogens and animals, have a considerable degree of resistance to those microbial and animal pathogens. The substances according to the invention can therefore be used to protect plants against attack by the pathogens mentioned above within a certain post-treatment period.

[0037] The plants and plant varieties of the Musaceae family that are preferably treated according to the invention include all plants that contain hereditary material that gives these plants particularly advantageous useful traits (regardless of whether this has been achieved by reproduction and / or biotechnology).

[0038] Plants and plant varieties of the Musaceae family that are also preferably treated according to the invention are resistant to one or more factors of biotic stress, that is, these plants have an enhanced defense against animal and microbial pathogens , such as nematodes, insects, mites, phytopathogenic fungi, bacteria, viruses and / or viroids. Those that should be mentioned preferably in this context are Musaceae which is resistant to fungi or phytopathogenic viruses.

[0039] The plants and plant varieties of the Musaceae family that can also be treated according to the invention are those plants that are resistant to one or more factors of abiotic stress. Abiotic stress conditions can include, for example, drought, low temperature and high temperature conditions, osmotic stress, flooding, increased soil salinity, increased exposure to minerals, ozone conditions, strong light conditions, limited availability of nutrients from nitrogen, limited availability of phosphorus nutrients or avoidance of shade.

[0040] The plants and plant varieties of the Musaceae family that can also be treated according to the invention are those plants in which vaccines or therapeutic proteins are expressed in a heterologous manner. These include, for example, hepatitis B antigen.

[0041] The plants and plant varieties of the Musaceae family that can also be treated according to the invention are those plants that are characterized by characteristics of enhanced productivity. In these plants, enhanced productivity can be caused, for example, by improved plant physiology, improved plant growth and improved plant development, such as water use efficiency, water retention efficiency, improved nitrogen utilization, carbon assimilation enhanced photosynthesis, improved germination vigor and accelerated maturation. Productivity can be further influenced by the improved plant architecture (under stress and non-stress conditions), including early flowering,

flowering control for hybrid seed production, seedling vigor, plant size, internal number and distance, root growth, seed size, fruit size, pod size, number of pod or ear, number of seeds per pod or ear, seed biomass, increased seed filling, reduced seed defoliation, reduced pod abortion and vertical force. Additional productivity-related traits include seed composition, such as carbohydrate content, protein content, oil content and oil composition, nutritional value, reduction in anti-nutritional compounds, improved processability and improved storage stability.

[0042] The plants of the Musaceae family that can be treated according to the invention are hybrid plants that already express the characteristics of heterolysis or hybrid vigor that generally results in greater productivity, vigor, health and resistance to biotic and abiotic stress factors. Such plants are typically made by crossing a male-sterile inbred parent line (female parent) with another male-sterile inbred parent line (male parent). Hybrid seed is typically harvested from male sterile plants and sold to growers. Sterile male plants can sometimes be (for example, in maize) produced by stripping, that is, the mechanical removal of male reproductive organs (or male flowers), but more typically, male sterility is the result of genetic determinants in the plant genome. In that case and especially when the seed is the desired product to be harvested from hybrid plants, it is typically useful to ensure that male fertility in hybrid plants that contain the genetic determinants responsible for male sterility is completely restored. This can be accomplished by ensuring that male parents have appropriate fertility restoring genes that are capable of restoring male fertility in hybrid plants that contain the genetic determinants responsible for male sterility. The genetic determinants for male sterility may be located in the cytoplasm. Examples of cytoplasmic male sterility (CMS) have, for example, been described for Brassica species. However, genetic determinants for male sterility can also be located in the nuclear genome. Sterile plants can also be obtained by methods of plant biotechnology such as genetic engineering. A particularly useful means of obtaining male-sterile plants is described in WO 89/10396, in which, for example, a ribonuclease, such as barnase is selectively expressed in tapetum cells in the stamens. Fertility can then be restored by expression in the tapetum cells of a ribonuclease inhibitor, such as barstar.

[0043] The plants or plant varieties of the Musaceae family (obtained by methods of plant biotechnology, such as genetic engineering) that can be treated according to the invention are herbicide tolerant plants, that is, plants made tolerant to one or more determined herbicides. Such plants can be obtained by genetic transformation, or by selecting plants that contain a mutation that gives such tolerance to herbicide.

[0044] Herbicide tolerant plants are, for example, glyphosate tolerant plants, that is, plants made tolerant to glyphosate herbicide or salts thereof. For example, glyphosate-tolerant plants can be obtained by transforming the plant with a gene encoding the enzyme 5-enolpyruvylchiquimate-3-phosphate synthase (EPSPS). Examples of such EPSPS genes are the AroA gene (mutant CT7) from the bacterium Salmonella typhimurium, the CP4 gene from the bacterium Agrobacterium sp., The genes encoding a Petunia EPSPS, a Tomato EPSPS or an Eleusin EPSPS. They can also be a mutated EPSPS. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate oxidoreductase enzyme. Glyphosate-tolerant plants can also be obtained by expressing a gene that encodes a glyphosate acetyltransferase enzyme. Glyphosate-tolerant plants can also be obtained by selecting plants that contain naturally occurring mutations of the genes mentioned above.

[0045] Other herbicide-resistant plants are, for example, plants that are made tolerant to herbicides that inhibit the enzyme glutamine synthase, such as bialafos, phosphinothricin or glufosinate. Such plants can be obtained by the expression of an enzyme that detoxifies the herbicide or a mutant of the enzyme glutamine synthase that is resistant to inhibition. An enzyme of such an efficient detoxifying enzyme is, for example, an enzyme encoding a phosphinothricin acetyltransferase (such as, for example, the bar or pat protein of the species Streptomyces). Plants that express an exogenous phosphinothricin acetyltransferase have been described.

[0046] Additional herbicide-tolerant plants are also plants that are made tolerant to herbicides that inhibit the hydroxyphenylpyruvate dioxigenase (HPPD) enzyme. Hydroxyphenylpyruvatodioxigenases are enzymes that catalyze the reaction in which parahydroxyphenylpyruvate (HPP) is transformed into homogentisate. Plants tolerant to HPPD inhibitors can be transformed with a gene encoding a naturally occurring resistant HPPD enzyme, or a gene encoding a mutated HPPD enzyme. Tolerance to HPPD inhibitors can also be obtained by transforming plants with genes that encode certain enzymes that allow the formation of homogentisate despite the inhibition of the native HPPD enzyme by the HPPD inhibitor. Plant tolerance to HPPD inhibitors can also be improved by transforming plants with a gene that encodes a prefenate dehydrogenase enzyme in addition to a gene that encodes an HPPD-tolerant enzyme.

[0047] Additional herbicide resistant plants are plants that are made tolerant to acetolactate synthase (ALS) inhibitors. Known ALS inhibitors include, for example, sulfonylurea herbicides, imidazolinone, triazolopyrimidines, pyrimidinyloxy (thio) benzoates and / or sulfonylaminocarbonyltriazolinone. Different mutations in the ALS enzyme (also known as acetohydroxy acid synthase, AHAS) are known to confer tolerance to different herbicides and groups of herbicides. The production of sulfonylurea tolerant plants and imidazolinone tolerant plants is described in the international publication WO 1996/033270. Additional imidazolinone and sulfonylurea tolerant plants are also described, for example, in WO 2007/024782.

[0048] Other plants that are tolerant to imidazolinone and / or sulfonylurea can be obtained by induced mutagenesis, selection in cell cultures in the presence of the herbicide or by reproduction by mutation.

[0049] Plants or plant varieties of the Musaceae family (obtained through methods of plant biotechnology, such as genetic engineering) that can also be treated according to the invention are transgenic plants resistant to insects, that is, plants made resistant to attack certain target insects. Such plants can be obtained by genetic transformation or by selecting plants that contain a mutation that confers such resistance to insects.

[0050] A "transgenic insect resistant plant", as used herein, includes any plant that contains at least one transgene that comprises a coding sequence that encodes: 1) an insecticidal crystal protein from Bacillus thuringiensis or an insecticidal portion of same, as the crystal insecticidal proteins described online at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt/, or insecticidal portions thereof, for example, proteins of the Cry Cry1Ab, Cry1Ac protein classes , Cry1F, Cry2Ab, Cry3Ae or Cry3Bb or insecticidal portions thereof; or 2) a Bacillus thuringiensis crystal protein or a portion of it that is insecticidal in the presence of a second Bacillus thuringiensis crystal protein or a portion thereof, such as the binary toxin composed of the crystal proteins Cy34 and Cy35; or 3) a hybrid insecticidal protein comprising parts of two different insecticidal crystal proteins from Bacillus thuringiensis, such as a hybrid of the proteins of 1) above or a hybrid of the proteins of 2) above, for example, the Cry1A.105 protein produced by the event of MON98034 maize (WO 2007/027777); or 4) a protein from any of points 1) to 3) above, where some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain greater insecticidal activity to a target insect species, and / or to expand the range of affected target insect species, and / or due to changes introduced in the coding DNA during cloning or transformation, such as the Cry3Bb1 protein in MON863 or MON88017 corn events, or the Cry3A protein in the MIR 604 corn event; 5) a secreted insecticidal protein from Bacillus thuringiensis or Bacillus cereus, or an insecticidal portion of it, such as the vegetative insecticidal proteins (VIP) listed at: http://www.lifesci.sussex.ac.uk/Home/Neil_Crickmore/Bt / vip.html, for example, proteins of the VIP3Aa protein class; or 6) a secreted protein from Bacillus thuringiensis or Bacillus cereus that is insecticidal in the presence of a second secreted protein from Bacillus thuringiensis or B. cereus, such as the binary toxin composed of the VIP1A and VIP2A proteins; or 7) a hybrid insecticidal protein comprising parts of different proteins secreted from Bacillus thuringiensis or Bacillus cereus, as a hybrid of the proteins in 1) or a hybrid of the proteins in 2) above; or 8) a protein from any one of points 1) to 3) above where some, particularly 1 to 10, amino acids have been replaced by another amino acid to obtain superior insecticidal activity for a target insect species, and / or for expand the range of affected target insect species, and / or because of changes introduced in the coding DNA during cloning or transformation (while still encoding an insecticidal protein), such as the VIP3Aa protein in a COT 102 cotton event.

[0051] Of course, a transgenic insect-resistant plant, as used in this document, also includes any plant that comprises a combination of genes that encode the proteins of any of the classes 1 to 8 above. In one embodiment, an insect-resistant plant contains more than a transgene that encodes a protein of any of the above classes 1 to 8, in order to expand the range of target insect species affected or to delay the development of insect resistance for plants, by using different insecticidal proteins for the same target species of insect, but having a different steel mode, as a link to different receptor binding sites in the insect.

[0052] Plants or plant varieties of the Musaceae family (obtained by plant biotechnology, such as genetic manipulation) that can also be treated according to the invention are tolerant to abiotic stress factors. Such plants can be obtained by genetic transformation or by selecting plants that contain a mutation that confers such resistance to stress. Particularly useful stress-tolerant plants include: a. Plants containing a transgene capable of reducing the expression and / or activity of the poly (ADP-

ribose) polymerase (PARP) in plant or plant cells. B. Plants that contain a stress tolerance-enhancing transgene capable of reducing the expression and / or activity of PARG that encodes plant or cell genes. ç. Plants that contain a transgene that enhances stress tolerance encoding a functional plant enzyme from the nicotinamide-adenine dinucleotide recovery biosynthesis pathway, including nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid adenyltransferase, nicotinamide dinotinamide, nicotinamide dinotinamide dinotinamide. Application forms

[0053] The treatment according to the invention of the plants of the Musaceae family and plant parts and propagation material with Isothianil is performed directly or by acting on its environment, habitat or storage by common treatment methods, for example, by dripping , spraying, atomization, fogging, spreading, painting, injection.

[0054] In an especially preferred embodiment of the present invention, Isothianyl or its formulations are used for application for the treatment of vegetative propagating material, or for rhizome or leaf application or drip application, especially preferred drip application, preferably drip application every 30 days, more preferably, every 14 days, preferably with 2.5 to 0.5 g of Fosetil-Al / plant and

0.035 to 0.015 g of IST / plant, more preferably, with 2.0 to 1.0 g of Fosetil-Al / plant and 0.03 to 0.02 g of IST / plant, even more preferably, with 2, 0 to 1.0 g of Fosetil-Al / plant and 0.03 to 0.02 g of IST / plant, and most preferably with 1.6 g of Fosetil-Al / plant and 0.024 g of IST / plant .

[0055] In another especially preferred embodiment of the present invention, Isothianyl or its formulations is used for application for the treatment of vegetative propagation material or for rhizome or leaf application or drip application, especially preferred drip application, preferably drip application every 30 days, more preferably every 14 days, preferably with 0.45 to 0.1 g of Fosetil-Al / plant and 0.04 to 0.015 g of IST / plant, more preferably , with 0.4 to 0.15 g of Fosetil-Al / plant and 0.04 to 0.02 g of IST / plant, even more preferably, with 0.35 to 0.25 g of Fosetil-Al / plant and 0.035 to 0.025 g of IST / plant and, most preferably, with 0.28 g of Fosetil-Al / plant and 0.028 g of IST / plant.

[0056] In an alternative embodiment of the present invention, Isothianyl or its formulations are used for application in the form of granules (for Fosetil-Al), for soil treatment.

[0057] In another especially preferred embodiment of the present invention, Isothianyl only as an ai or its formulations are used for the treatment of vegetative propagating material or for rhizome or foliar application or drip application, especially preferred drip application, preferably every 30 days, more preferably every 14 days, preferably

0.035 to 0.015 g IST / plant, more preferably, with 0.03 to 0.02 g IST / plant, even more preferably, 0.03 to 0.02 g IST / plant and, most preferably , with 0.024 g IST / plant.

[0058] In another especially preferred embodiment of the present invention, Isothianyl only as an ai or its formulations is used for the treatment of vegetative propagating material or for rhizome or leaf application or drip application, especially preferred drip application, preferably every 30 days, more preferably every 14 days, with preferably 0.04 to 0.015 g of STI / plant, more preferably with 0.04 to 0.02 g of STI / plant, still more preferably, with 0.035 to 0.025 g of IST / plant and, most preferably, with 0.028 g of IST / plant

[0059] In a preferential modality, the first treatment is performed 15 days before planting regardless of the following treatment intervals.

[0060] Depending on their respective physical and / or chemical properties, Isothianyl can be converted into common formulations, such as solutions, emulsions, suspensions, powders, foams, ointments, granules, sachets, aerosols, microencapsulations in polymeric substances and blurring formulations hot and cold ULV.

[0061] These formulations are prepared in a known manner, for example, by mixing Isothianil with extenders, that is, solvents, pressurized liquefied gases and / or liquid carriers, optionally, with the use of surfactants, that is, emulsifiers and / or dispersants and / or foaming agents. If water is used as an extender, it is also possible, for example, to use organic solvents as co-solvents.

The liquid solvents that are suitable are mainly: aromatics like xylene, toluene or alkylnaphthalenes, chlorinated aliphatic hydrocarbons or chlorinated aromatics like chlorobenzenes, chloroethylenes or methylene chloride, aliphatic hydrocarbons like cyclohexane or paraffins, for example, mineral oil fractions, alcohols like butanol or glycol and its ethers and esters, ketones like acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents like dimethylformamide and dimethyl sulfoxide, and water, and also mineral, animal and vegetable oils, for example , palm oil or other plant seed oils.

Liquefied gaseous extenders or carriers are understood as those liquids that are gaseous at normal temperature and under normal pressure, for example, aerosol propellants, such as halohydrocarbons and butane, propane, nitrogen and carbon dioxide.

Suitable solid carriers are: for example, natural terrestrial minerals, such as kaolines, clays, talc, chalk, quartz, atapulgite, montmorillonite or diatomaceous earth, and synthetic terrestrial minerals such as highly dispersed silica, alumina and silicates.

The solid carriers suitable for granules are: for example, crushed and fractionated natural rocks, such as calcite, pumice, marble, sepiolite, dolomite, and also synthetic granules of inorganic and organic flours, and granules of organic material such as sawdust, bark of coconut, corn cobs and tobacco stems.

Suitable emulsifiers and / or foams are: for example, nonionic, cationic and anionic emulsifiers, such as polyoxyethylene fatty acid esters,

polyoxyethylene fatty alcohol ethers, for example, alkylaryl polyglycol ethers, alkylsulfonates, alkyl sulfates, arylsulfonates and also protein hydrolysates. Suitable dispersants are: for example, residual lignosulfonate and methylcellulose liquors.

[0062] Adhesives, such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latex, such as gum arabic, polyvinyl alcohol, polyvinyl acetate and natural phospholipids, such as cephalins and lecithins and synthetic phospholipids, can be used in formulations. Additional additives can be mineral and vegetable oils.

[0063] It is possible to use dyes such as inorganic pigments, for example, iron oxide, titanium oxide, Prussian Blue, and organic dyes such as alizarin, azo and metal phthalocyanine dyes, and trace nutrients such as iron, manganese salts , boron, copper, cobalt, molybdenum and zinc.

[0064] In general, formulations contain between 5 and 95% by weight of active substance, preferably between 10 and 70% by weight of active substance, more preferably between 15 and 30% by weight of active substance, and , with maximum preference, 20% by weight of active substance.

[0065] The control of microbial and animal pathogens through the treatment of vegetative propagating material from plants has been known for a long time and is the object of continuous improvements. However, the treatment of vegetative propagation material involves a series of problems that are not always solved in a satisfactory manner. Thus, it is desirable to develop methods to protect material from vegetative propagation and germinating plants that end up with, or at least markedly reduce, the additional application of plant protection products after planting or after plant emergence. It is additionally desirable to optimize the amount of active substance used so that the vegetative propagating material and the germinating plant are protected as best as possible from attack by microbial pathogens, however, damaging the plant itself by the active substance used. In particular, methods for the treatment of vegetative propagation material must also consider the intrinsic properties of transgenic plants in order to achieve optimal protection of the vegetative propagation material and the germinating plant while maintaining the application rate of plant protection products. plant as low as possible.

[0066] Therefore, the present invention also relates, in particular, to a method of protecting vegetative propagation material and plants from germinating attack by microbial pathogens and animals, by treating seeds and vegetative propagation material with a composition according to the invention.

[0067] The invention also relates to the use of the compositions according to the invention for the treatment of vegetative propagation material to protect the vegetative propagation material and the germinating plant from microbial and animal pathogens.

[0068] One of the advantages of the present invention is that, due to the special systematic properties of the compositions according to the invention, the treatment of vegetative propagating material with these compositions protects not only the vegetative propagating material itself, but also the plants that arise after planting microbial pathogens and animals. In this way, immediate treatment of the crop at the time of planting, or soon after, can be dispensed with.

[0069] Another advantage is that the compositions according to the invention can also be used, in particular, in transgenic vegetative propagation material.

[0070] The compositions according to the invention are suitable for protecting vegetative propagating material from any variety of plant that is used in agriculture, greenhouse, forests or horticulture. In particular, this is the vegetative propagation material of Musaceae.

[0071] Within the scope of the present invention, the composition according to the invention is applied to the vegetative propagating material alone in a suitable formulation. Preferably, the vegetative propagating material is treated in a state in which it is sufficiently stable so that no damage occurs during treatment. In general, vegetative propagating material can be treated at any point in time between harvest and planting. Usually, vegetative propagation material that has been separated from the plant free of ears, peels, stems, coatings, hair or fruit pulp is used.

[0072] When treating vegetative propagation material, care should be taken, in general, with the amount of the composition according to the invention, and / or additional additives, applied to the vegetative propagation material that is chosen so that the germination of vegetative propagation material is not adversely affected, or the plant that emerges is not damaged. This should be considered, in particular, in the case of active substances which, at certain rates of application, can have phytotoxic effects.

[0073] The compositions according to the invention can be applied directly, that is, without containing additional components and without having been diluted. As a rule, it is preferable to apply the compositions to the vegetative propagating material in the form of a suitable formulation. Formulations and methods suitable for the treatment of seeds and vegetative propagating material are known by the versed element.

[0074] The compounds that can be used according to the invention and that are selected from the compounds according to formula (I) can be converted into common formulations, such as solutions, emulsions, suspensions, powders, foams and ULV formulations .

[0075] These formulations are prepared in a known manner by mixing the compounds selected from the compounds of formula (I) with common additives, such as, for example, common extenders and also solvents or diluents, dyes, humidifiers, dispersants, emulsifiers, defoamers , preservatives, secondary thickeners, adhesives, gibberellins, mineral and vegetable oils and also water.

[0076] The dyes that can be present in the formulations that can be used according to the invention are all dyes that are common for such purposes. In this context, both pigments, which are sparingly soluble in water, and dyes, which are soluble in water, can be used. Examples that can be mentioned are the dyes known by the names Rhodamine B, C.I. Pigment Red 112 and C.I. Solvent Red 1.

[0077] Humidifiers that may be present in formulations that can be used according to the invention are all substances that are common in the formulation of agrochemical active substances and that promote moistening. Alkylnaphthalenesulfonates, such as diisopropyl- or diisobutylaphthalenesulfonates, can preferably be used.

[0078] Suitable dispersants and / or emulsifiers that may be present in formulations that can be used according to the invention are all nonionic, anionic and cationic dispersants that are conventionally used for the formulation of agrochemical active substances. The following can be used with preference: nonionic or anionic dispersants or mixtures of nonionic or anionic dispersants. Suitable non-ionic dispersants that can be mentioned are, in particular, ethylene oxide / propylene oxide block polymers, polyglycolic alkylphenol ethers and polyglycolic tristyrylphenol ethers and their phosphate or sulfate derivatives. Suitable anionic dispersants are, in particular, lignosulfonates, polyacrylic acid salts and condensed arylsulfonate / formaldehyde.

[0079] The defoamers that may be present in the formulations that can be used according to the invention are all foam inhibiting substances that are conventionally used for the formulation of agrochemical active substances. Silicone defoamers and magnesium stearate can be used with preference.

[0080] The preservatives that can be present in the formulations that can be used according to the invention are all substances that can be used for such purposes in agrochemical compositions. Examples that can be mentioned are dichlorophene and hemiformal benzyl alcohol.

[0081] The secondary thickeners that can be present in the formulations that can be used according to the invention are all substances that can be used for such purposes in agrochemical compositions. Cellulose derivatives, derivatives of acrylic acid, xanthan, modified clays and highly dispersed silicas are preferably suitable.

[0082] Adhesives can be present in formulations that can be used according to the invention are all common binders that can be used in mordants. Polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol and tylose can be mentioned with preference.

[0083] The gibberellins which may be present in the formulations which can be used according to the invention are preferably Gibberellin A1, Gibberellin A3 (gibberellic acid), Gibberellin A4, Gibberellin A7. Especially preferred gibberellin is gibberellic acid.

[0084] Gibberellins are known (see R. Wegler "Chemie der Pflanzenschutz- und Schädlingsbekämpfungsmittel" [Plant protection chemistry and pesticide agents], volume 2, Springer Verlag, Berlin-Heidelberg-New York, 1970, pages 401-412) .

[0085] The formulations that can be used according to the invention can be used for the treatment of various types of seeds, directly or previously after having been diluted with water. Thus, concentrates or preparations obtained from them by dilution with water can be used to coat Musaceae seeds. The formulations that can be used according to the invention or their diluted preparations can also be used to treat the vegetative propagation material of transgenic plants. Here, additional synergistic effects can also occur in combination with substances formed by expression.

[0086] The application rate of the formulations that can be used according to the invention can be varied within a substantial range. It depends on the respective active substance content in the formulations and on the vegetative propagation material. As a rule, application rates of active substance are preferably between 0.001 and 50 g per kilogram of vegetative propagating material, more preferably between 0.01 and 15 g per kilogram of vegetative propagating material. Mixtures

[0087] A compound selected from the compounds according to formula (I) can be used as such or, in the formulations, also in a mixture with fungicides, bactericides, acaricides, nematicides, herbicides, insecticides, protection agents, products for improvement of soil or products to reduce stress in known plants, for example, Miconato, in order to broaden the spectrum of action or prevent the development of resistance, for example. In many cases, this generates synergistic effects, that is, the effectiveness of the mixture exceeds the effectiveness of the individual components.

[0088] In a preferred embodiment, the present invention in a mixture of Isothianyl with at least one additionally active ingredient selected from Fosetil-Al, and mono- and dibasic sodium, potassium and ammonium phosphites (for example, Phostrol), more preferably, Fosetil-Al; wherein the ratio by weight% of Isothianyl to the mixing partner is preferably from 1 to 20 to 1 to 100, more preferably from 1 to 40 to 1 to 80, even more preferably from 1 to 60 to 1 to 75.

[0089] In another preferred embodiment, the present invention is a mixture of Isothianyl with at least one additionally active ingredient selected from Fosetil-Al, and mono- and dibasic sodium, potassium and ammonium phosphites (for example, Phostrol) more preferably, Fosetil-Al; wherein the ratio by weight% of Isothianyl to the mixing partner is preferably 1 to 1 to 1 to 50, more preferably 1 to 3 to 1 to 30, even more preferably 1 to 5 to 1 to 15.

[0090] According to the invention, the term "mixture" means various combinations of at least two of the aforementioned active substances that are possible, such as, for example, ready-mixes, tank mixes (which is meant by spray sprays) prepared from the formulations of the individual active substances by combining and diluting before application) or combinations thereof (for example, a binary prone mixture of two of the aforementioned active substances is made in a tank mixture when using a formulation of the third individual substance) . According to the invention, the individual active substances can also be used sequentially, that is, one after the other, within a reasonable interval of a few hours or days, in the case where the seed treatment, for example, also when applying a plurality of layers containing different active substances. Preferably, it is immaterial in which order the individual active substances can be used.

[0091] The compounds according to the formula (I) can be used as, in the form of their formulations or in the forms of use prepared from them, as ready-to-use solutions, suspensions, wetable powders, ointments, soluble powders, dust and granules. They are applied in the common way, for example, by pouring, spraying, atomizing, spreading, dusting, foaming, painting and the like. It is also possible to apply the compounds according to formula (I) using the ultra low volume method or to inject the active substance preparation or the active substance itself into the soil. The vegetative propagation material of the plants can be treated as well.

[0092] When employing a compound selected from the compounds according to formula (I), application rates can be varied within a substantial range depending on the type of application. In the treatment of plant parts, the application rates of active substance are preferably between 0.1 and 10,000 g / ha, more preferably between 10 and 1000 g / ha. In the treatment of vegetative propagating material, the application rates of active substance are preferably between 0.001 and 50 g per kilogram of vegetative propagating material, more preferably between 0.01 and 10 g per kilogram of propagating material. vegetative. In soil treatment, application rates of active substance are preferably between 0.1 and 10,000 g / ha, more preferably between 1 and 5000 g / ha.

[0093] It is intended that the following examples illustrate the invention, but without imposing any limitations. Examples: Example 1 Effectiveness of Isothianyl and Fosetil AL against Fusarium oxysporum Race 4

[0094] This example illustrates the effectiveness of compositions containing Isothianil against Fusarium oxysporum Race 4 on Cavendish bananas.

[0095] The test was carried out in Randomized Complete Block Design (RCBD) with four treatments (10 plants / treatment) with one) Untreated, b) Aliette 80WG 2 g / plant (FEA 1.6 g ai / plant), c ) Isothianyl SC200 g / l 0.12 ml / plant (IST 0.024 g ai / plant) and d) Aliette 80WG + Isothianil SC200 - 2 g + 0.12 ml / plant (1.6 + 0.024 g ai / plant).

[0096] The applications were carried out in soil baths at monthly intervals Results Infection by Fusarium disease affected by the application of different fungicides Treatment Rate / plant Method / number of Days in which% of frequency of plants the symptoms of infection Control of application infected b) 2 grams drainage of 7 112 70 30 soil / monthly c) 0.12 ml drainage of 5 112 50 50 soil / monthly d) 2 grams + drainage of 1 245 10 90 0.12 ml soil / monthly a) - - 10 90 100 0

[0097] Test 1: The result of the initial test shows that Aliette at 2 grams + Isothianyl 0.12 ml / plant applied as a preservative as soil drainage at monthly intervals generated 90% protection from fusarium infection. While Isothianyl in 0.12 ml and Aliette at 2 grams / plant applied as soil controls infection by 30% and 50% respectively. Under these conditions, a synergy could be measured between the two compounds (Colby formula - Efficacy calculated by Abbott: 65%). In addition, the occurrence of symptoms in treated plants was delayed by 22 days (soil with Aliette and Isotianila) and 155 days (Aliette + Isotianila) in relation to UTC. Example 2 Effectiveness of Isothianyl and Isothianyl + Fosetil AL against Fusarium oxysporum Race 1

[0098] This example illustrates the efficacy of compositions containing Isothianil against Fusarium oxysporum Race 1 in the Variety Gros Michel banana.

[0099] The test was carried out in Randomized Complete Block Design (RCBD) with three blocks and eight replicates per block - Total treatments with e) Untreated, f) Aliette 80WG 2 g / plant (FEA 1.6 g ai / plant) , g) Isothianyl SC200 g / l 0.12 ml / plant (IST 0.024 g ai / plant) eh) Aliette 80WG + Isothianil SC200 - 2 g + 0.12 ml / plant (1.8 + 0.024 g ai / plant) .

[0100] The applications were carried out in soil drainage, starting in the nursery 15 days before planting and continuing at the time of planting followed by monthly intervals (applications at the base of the plant and around 40 cm). The evaluations performed were% of infected plants and Severity of infection in trunks at the end of the test period (Severity Scale Levels 0 = no symptoms at 4 = dead plant). Results of a test in Costa Rica 2015/2016 Treatment Rate by% Incidence Rate% Incidence% Severity plant ai / pla 90 days after 180 days after 3 plants in planting (% planting (% of cut efficiency effectiveness) from (tall trunk) Abbott) Abbott) e) 81.7 100 2.3 f) 2 g / Plant 1.6 g 60 (27) 93.3 (6.7) 3.1 g) 0.12 ml / plant 0.024 g 46.7 (43) 70 (30) 2.2 ah) 2 g + 0.12 ml / p 1.6 g + 0 (100) 13.3 (87.7) 0.7 lanta 0.024 g

[0101] Test 2: The result of the final test shows that Aliette at 2 grams + Isothianyl 0.12 ml / plant applied as a preservative as soil drainage at monthly intervals generated 100% protection from fusarium infection 90 days after planting. While Isothianyl in 0.12 ml and aliette at 2 grams / plant applied as soil controls the infection by 43% and 27% respectively. Under these conditions, a synergy could be measured between the two compounds (Colby formula - Abbott's calculated effectiveness: 58%). In addition, despite the lower persistence of single compounds, the mixture shows a high level of control 180 days after planting and reduces the severity of infection at the highest stem levels. Example 3 Greenhouse test - efficacy of Isothianil + Fosetil AL against Fusarium oxysporum Race 4

[0102] This example illustrates the effectiveness of a composition counting Isothianyl against Fusarium oxysporum Race 4 in banana trees Cavendish Variety Grande Naine.

[0103] The test in the Greenhouse with 30 plants per treatment (3 replicates of 10 plants each). The two-month banana trees were transplanted into the infested soil. The compounds were drained 6 days before replanting (protective application). A second application was performed 4 weeks after the first application. The applications were performed with j) Aliette 80WG 2 g / plant (FEA 1.6 g ai / plant) and k) Aliette80WG + Isothianyl SC200 - 2 g + 0.12 ml / plant (1.8 + 0.024 g ai / plant) compared to i) untreated contaminated plants.

[0104] Scoring was performed six weeks after inoculation according to an internal plant discoloration scale (typical symptom for Fusarium oxysporum). Scale levels 0 = no symptoms at 6 = total necrosis of the plant) Results Treatment Rate per plant Score rate Score ai / plant of replicates Average (0-6) 3 (0-6) i) 6-6-6 6 j) 2 g / Plant 1.6 g 5.6-5.3-5.1 5.3 k) 2 g + 0.12 ml / plant 1.6 g + 1.5-1.7-1.8 1.7 0.024 g

[0105] Test 3: The final test result 6 weeks after inoculation shows significant differences in the protective protection between i) Untreated contaminated (Average of 3 replicates = 6), j) Aliette 2 g / plant (T1C1 average of 3 replicates = 5.3)

and k) Aliette + Isothianyl 2 g + 0.12 ml / plant (Trt T4C1 average of 3 replicates = Score 1.7). The mixture of Fosetil AL + Isothianil provides good protection against Foc in these conditions of very intense infection when Aliette was not so active, showing the interest of mixing it with Isothianil. Example 4 - Application of soil - application of ratio 2 tested Effectiveness of Isothianyl and Fosetil AL (ratio 60: 1 and ratio 10: 1) against Fusarium oxysporum Race 4 applied in drainage

[0106] This example illustrates the effectiveness of the two compositions containing fosetil AL + Isotianila applied in the drainage against Fusarium oxysporum Race 4 in the banana plantation.

[0107] The tests were performed in the plantation (3 replicates / treatment - 20 plants evaluated / Plotting) in Randomized Complete Block Design (RCBD) in the Cavendish variety, with untreated Fosetil AL + Isothianyl (SP102000028595 WG77% - Reason 10: 1) - 0.4 g / Plant (0.3 + 0.03 g ai / plant), Fosetil + Isothianyl (SP102000033663 WG76.5% - 60: 1 ratio) - 0.4 g / Plant (0.3+ 0.005 g / plant). The rates of formulation 2 were calculated to bring the amount of Fosetil AL and the variable amount of Isothianyl. All applications of FEA + IST were performed in the drainage application at a monthly interval with 500 ml of water volume per plant.

[0108] The results of the initial test at 2 sites show that the use of FEA + IST in the drainage application method, whatever the 10: 1 or 60: 1 ratio, significantly delays the evolution of Fusarium oxysporum cubensis breed 4. This delay in the evolution of the disease is reflected in the preservation of the production potential in the treated plots. Results of the two tests: Treatment Rate by% Incidence Rate% Incidence of plant ai / plant of plants infected plants of test 1 after test 2 after last application last 54DAT3 (M) application 29DAT3 (M) 1-No treated 30 (a) 65.9 (a) 10-Ratio 10: 1 0.4 g / Plant 0.28 + 0.028 g 12.1 (bc) 41.7 (b)

ALIETTE + Isothianyl WG77% 13-Ratio 60: 1 0.4 g / Plant 0.3 + 0.005 g 12.9 (bc) 28.9 (b)

ALIETTE + Isotianila WG76.25% Treatment Rate by Yield Rate plant ai / plant Test 1 in% of Test 2 UTC (UTC = in% of UTC 12.4T / Ha) 182DAT3 (UTC = (M) 5, 3T / Ha) 178DAT3 (M) 1-Untreated 100 100 10-Ratio 10: 1 0.4 g / Plant 0.28 + 0.028 178.1 164.2 ALIETTE ag + Isothianyl WG77% 13-Ratio 60: 1 0 , 4 g / Plant 0,3 + 0,005 g 168 209,4 ALIETTE a + Isothianil WG76,25% Example 5 - Leaf application - Ratio 2 Tested Isotianil and Fosetil AL efficacy (60: 1 ratio and 10: 1 ratio)

against Fusarium oxysporum Race 4 applied in leaf sprinkling

[0109] This example illustrates the effectiveness of the two compositions containing fosetil AL + Isothianil applied in leaf sprinkling against Fusarium oxysporum Race 4 in the banana plantation.

[0110] The tests were carried out on the plantation (3 replicates / treatment - 20 plants evaluated / Plotting) in Randomized Complete Block Design (RCBD) in the Cavendish variety, with untreated Fosetil AL + Isotianil (SP102000028595 WG77% - Reason 10: 1) - 2 g / Plant (1.4 + 0.14 g ai / plant), Fosetil + Isothianyl (SP102000033663 WG76.5% - 60: 1 ratio) - 2 g / Plant (1.5 + 0.025 g / plant ). All applications of FEA + IST were performed in foliar application at a monthly interval with 50 ml of water volume per plant.

[0111] The results of the initial test at the 2 sites show that the use of FEA + IST by leaf sprinkling, whatever the 10: 1 or 60: 1 ratio, significantly delays the evolution of Fusarium oxysporum cubensis race 4. This delay of evolution of the disease is reflected in the preservation of the production potential in the treated plots. Results of the two tests: Treatment Rate by% Incidence Rate of% Incidence of plant ai / plant infected plants infected plants from test 1 after test 2 after last application last application 54DAT6 (M) 29DAT6 (M) 1-No treated 30 (a) 65.9 (a) 2-Ratio 10: 1 2 g / Plant 1.4 + 0.14 g 9.6 (b) 16.7 (b)

ALIETTE + Isothianyl WG77%

Treatment Rate by% Incidence Rate% Incidence of plant ai / plant infected plants infected plants from test 1 after test 2 after last application last application 54DAT6 (M) 29DAT6 (M) 3-Ratio 60: 1 2 g / Plant 1.5 + 0.025 g 11.7 (b) 32.3 (b)

ALIETTE + Isotianila WG76.25% Treatment Rate by Yield Rate of plant ai / plant Test 1 in% of Test 2 in% of UTC (UTC = UTC (UTC = 12.4T / Ha) 5.3T / Ha) 178DAT6 182DAT6 (M) (M) 1-Untreated 100 100 2-Ratio 10: 1 2 g / Plant 1.4 + 0.14 g 157.7 282.5

ALIETTE + Isothianyl WG77% 3-Ratio 60: 1 2 g / Plant 1.5 + 0.025 g 190.1 174.4

ALIETTE + Isotianila WG76.25% Conclusion for the protection of banana plantations against Fusarium oxysporum responsible for Fusarium wilt

[0112] The five reported examples confirm the interest in using Isothianyl-based compounds to limit the development of Fusarium oxysporum Race 4 in banana trees. The mixture between Isotianil and Fosetil AL shows even better protection in field plantations. Ratio 2 tested in the application of drainage and leaf sprinkling shows a significant reduction in disease infestation and will allow, under certain conditions, to delay the development of Panama disease in the banana plantation. The advantage for the producer is translated into a better survival of the plantation plantation with less infected plants and an increased yield.

Claims (17)

1. Use characterized by being of Isothianyl (formula (I)) Cl Cl N H S N CN (I) to control Panama disease in plants of the Musaceae family. 2. Use of Isothianil, according to claim 1, characterized for being to control fungi of the Fusarium oxysporum spp family. in plants of the Musaceae family. Use of Isothianyl, according to claim 1 or 2, characterized in that it is for controlling fungi of Fusarium oxysporum f. sp. cubense in plants of the Musaceae family. 4. Use of Isothianyl according to any one of the preceding claims, characterized in that it controls Fusarium oxysporum f. sp. cubense in plants of the Musaceae family. 5. Use of Isothianyl, according to any one of the preceding claims, characterized in that it is used to control fungi of the Fusarium oxysporum f. sp. cubense in plants of the Musaceae family. 6. Use of Isothianil, according to any of the previous claims, characterized by the fact that the plant of the Musaceae family is selected from the variety of Cavendish or Gros Michel. 7. Use of Isothianyl, according to any of the preceding claims, characterized by the fact that Isothianyl is used in combination with Fosetil-Al. 8. Use of Isothianyl according to any one of the preceding claims, characterized by the fact that Isothianyl is used in combination with Fosetil-Al in a weight% ratio of 1 to 60 to 1 to 75. 9. Use of Isothianyl according to any one of the preceding claims, characterized by the fact that Isothianyl is used in combination with Fosetil-Al in a weight% ratio of 1 to 5 to 1 to 15. 10. Panama disease control method in plants of the Musaceae family characterized by the fact that the plants of the Musaceae family are treated with Isothianyl (I). 11. Method, according to claim 10, characterized by the fact that the plants of the Musaceae family are treated with Isothianyl (I) in combination with Fosetil-Al. 12. Method, according to claims 10 and 11, characterized by the fact that the first treatment is carried out 15 days before planting. 13. Method according to claims 10 and 11, characterized by the fact that the treatments are carried out in an interval of 30 days, preferably in an interval of 14 days. 14. Method according to claims 10 to 13, characterized in that the amount of ai per plant per treatment is 2.5 to 0.5 g of Fosetil-Al and 0.035 to 0.015 g Isothianyl or a combination of both . 15. Method according to claims 10 to 13, characterized in that the amount of ai per plant per treatment is 0.45 to 0.0.1 g of Fosetil-Al and 0.04 to 0.015 g Isothianyl or a combination of both. 16. Method according to claim 11, characterized in that the ratio in weight% between Isothianyl and Fosetil-Al is 1 to 60 to 1 to 75. 17. Method according to claims 10 to 16, characterized by the fact that the application is a drip application.