CN111260099B - Liquid milk shelf life prediction method and prediction system - Google Patents
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- 235000013336 milk Nutrition 0.000 title claims abstract description 67
- 210000004080 milk Anatomy 0.000 title claims abstract description 67
- 239000008267 milk Substances 0.000 title claims abstract description 66
- 239000007788 liquid Substances 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000004519 manufacturing process Methods 0.000 claims abstract description 21
- 238000001514 detection method Methods 0.000 claims description 8
- 238000004364 calculation method Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 description 12
- 235000013361 beverage Nutrition 0.000 description 10
- 238000004659 sterilization and disinfection Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 8
- 238000004590 computer program Methods 0.000 description 7
- 235000020191 long-life milk Nutrition 0.000 description 6
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- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000013124 brewing process Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000020122 reconstituted milk Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The invention relates to a method and a system for predicting the shelf life of liquid milk. The prediction method comprises the following steps: (1) Detecting Zeta potentials Z 1、Z2, Z 3,t1、t2 and t 3 of the liquid milk as positive integers and t 1＜t2＜t3 respectively at t 1、t2 and t 3 days from the production day; (2) Substituting t=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 into the following equation |Z|=kln (h-t) +c, establishing an equation set, and solving an unknown variable h; and (3) taking the h day from the production day as the predicted value of the shelf life of the liquid milk. The method has the advantages of simplicity, rapidness and accuracy.
Description
Technical Field
The invention relates to the field of liquid milk quality control, in particular to a liquid milk shelf life prediction method and a liquid milk shelf life prediction system.
Background
Food shelf life (shell-life) refers to the period of time during which the food is stored under recommended conditions, can remain safe, ensure desirable organoleptic, physicochemical and microbiological properties, and retain any nutritional value declared by the tag.
In recent years, various manufacturers have been devoted to studies for prolonging the shelf life of products, particularly Extended Shelf Life (ESL) milk, which is widely paid attention to at home and abroad, in order to obtain sales benefits.
In the related art, ultra-high temperature (Ultra High Temperature, UHT) sterilized milk can be obtained by ultra-high temperature instantaneous sterilization (e.g., 135-142 ℃ C./4 s). The ultra-high temperature instantaneous sterilization can kill all pathogenic bacteria and spores in milk, passivates all enzyme systems, and can be filled under aseptic conditions, thereby meeting the requirements of commercial sterility.
Some infant liquid milks are obtainable by ultra high temperature flash sterilization (UHT). The infant liquid dairy product can meet the aseptic requirement, and avoids pollution caused by containers and operations in the traditional brewing process. The various nutrient components are stable, uniform and consistent, do not need to be mixed, and are convenient to eat. Reduces the risk of adverse reactions caused by improper feeding,
Some milk-containing beverages can be obtained by ultra high temperature flash sterilization (UHT). Some milk-containing beverages have milk content of above 30% and protein and fat content of above 1%.
The shelf life of the liquid dairy product obtained by the ultra-high temperature sterilization method is generally more than 6 months, but at present, no method for accurately predicting the shelf life of the dairy product exists in both countries and enterprises, and the shelf life of the liquid dairy product is determined mainly by placing the product under normal temperature conditions and periodically observing the product. The result time of this method of determination is long, and it usually takes 6-8 months to verify the accuracy of the determination.
Disclosure of Invention
The present disclosure provides a novel method of predicting the shelf life of liquid milk.
In some aspects, a method of predicting shelf life of liquid milk is provided, comprising the steps of:
(1) Detecting Zeta potentials Z 1、Z2 and Z 3 of the liquid milk at t 1、t2 and t 3 days from the production day, respectively, wherein t 1、t2 and t 3 are positive integers, and t 1＜t2＜t3;
(2) T=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 is substituted into the following equation,
|Z|＝k ln(h-t)+c
And (3) establishing an equation set (such as a ternary cubic equation set), and solving the value of the unknown variable h to obtain the h day from the production day as the predicted value of the shelf life of the liquid milk.
In the equation |z|=kln (h-t) +c, k, c and h are all unknown variables, and therefore, when solving the equation set, the values of k and c can also be found.
In some embodiments, t 1 = 5-110, e.g., 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100.
In some embodiments, t 2-t1 > 10.
In some embodiments, t 3-t2 > 10.
In some embodiments, t 2-t1 = 20-40, e.g., 30.
In some embodiments, t 3-t2 = 20-40, e.g., 30.
In some embodiments, the detection methods of the present disclosure are performed at room temperature. For example at 10 to 30 ℃.
In some embodiments, the disclosed predictive methods are methods of predicting the shelf life of UHT sterilized liquid milk.
In some aspects, a liquid milk shelf life prediction system is provided, comprising
The Zeta potential detection module is used for respectively detecting Zeta potentials Z 1、Z2 and Z 3 of the liquid milk at the time t 1、t2 and t 3 from the production day, wherein t 1、t2 and t 3 are positive integers, and t 1＜t2＜t3;
A calculation module, substituting t=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 into the following equations,
|Z|＝k ln(h-t)+c
And (3) establishing an equation set, and solving an unknown variable h value, wherein the h day from the production day is a predicted value of the shelf life of the liquid milk.
In some embodiments, the predictive system of the present disclosure is a UHT sterilized liquid milk shelf life predictive system.
In some aspects, there is provided an apparatus for predicting shelf life of liquid milk comprising:
A memory; and
A processor coupled to the memory, the processor configured to perform the method of any of the present disclosure based on instructions stored in the memory.
In some embodiments, the zeta potential is obtained by zeta potential (zemoeid, zetasizer Nano SZE) detection in the present disclosure.
In some embodiments, the liquid milk of the present disclosure is UHT-sterilized liquid milk, e.g., UHT-sterilized fresh milk, e.g., UHT-sterilized milk-containing beverage.
UHT sterilization (Ultra-high temperature instantaneous sterilization), i.e. Ultra-high temperature instantaneous sterilization, is a sterilization process.
In some embodiments, the protein content in the liquid milk of the present disclosure is greater than 1wt%, such as greater than 2wt%, such as from 1 to 5wt%.
In some embodiments, the fat content in the liquid milk of the present disclosure is greater than 1wt%, e.g., > 2wt%, e.g., 1-5 wt%.
In some embodiments, UHT sterilized liquid milk is a liquid product prepared from raw cow (sheep) milk, with or without reconstituted milk, by heating to at least 132℃ (e.g., 135-140℃) in a continuous flow, sterilizing for a short period of time (e.g., 4-10 s), and aseptically filling.
In some embodiments, the UHT sterilized milk-containing beverage is a beverage product obtained by taking milk or a dairy product as a raw material, adding water and a proper amount of auxiliary materials, preparing or fermenting, and then UHT sterilizing.
In some embodiments, the milk-containing beverage of the present disclosure is in accordance with GB/T21032-2008 milk-containing beverage.
In some embodiments, the UHT-sterilized liquid milk of the present disclosure conforms to GB 25190-2010 sterilized milk.
Advantageous effects
The disclosed methods have one or more of the following benefits:
(1) The method is simple;
(2) The speed is high;
(3) The cost is low;
(4) The accuracy of the result is high.
The prediction method and system disclosed by the disclosure can rapidly predict the shelf life of UHT sterilized liquid milk. Based on the method, researchers can preliminarily determine the shelf life of the liquid milk sample without carrying out long experiments for more than 6 months, so that the efficiency of screening the sample can be accelerated, and the experimental process is accelerated. The deviation between the method and the actual experimental result is only 8.5-13.3%, and the predicted result has high reference value.
Drawings
FIG. 1 is a flow chart of a method of predicting the shelf life of liquid milk;
FIG. 2 is a schematic diagram of a system for predicting the shelf life of liquid milk;
fig. 3 is a schematic view of an apparatus for predicting shelf life of liquid milk.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Fig. 1 is a flow chart of a method of predicting shelf life of liquid milk.
As shown in fig. 1, in some aspects, a method of predicting the shelf life of liquid milk is provided, comprising the steps of:
Step 101, detecting Zeta potentials Z 1、Z2, Z 3,t1、t2 and t 3 of liquid milk respectively as positive integers and t 1＜t2＜t3 in t 1、t2 and t 3 days from the production day;
In step 102, t=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 is substituted into the following equation,
|Z|＝k ln(h-t)+c
And (3) establishing an equation set, and solving the value of the unknown variable h, wherein the h day from the production day is the predicted value of the shelf life of the liquid milk.
Z represents the absolute value of Z.
Fig. 2 is a schematic diagram of a system for predicting shelf life of liquid milk.
As shown in FIG. 2, a liquid milk shelf life prediction system comprises
The Zeta potential detection module 201 is configured to detect Zeta potentials Z 1、Z2, Z 3,t1、t2, and t 3 of the liquid milk as positive integers, respectively, and t 1＜t2＜t3, at t 1、t2 and t 3 days from the production day;
A calculation module 202 for substituting t=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 into the following equations,
|Z|＝k ln(h-t)+c
And (3) establishing an equation set, and solving the value of the unknown variable h, wherein the h day from the production day is the predicted value of the shelf life of the liquid milk.
Fig. 3 is a schematic view of an apparatus for predicting shelf life of liquid milk. As shown in fig. 3, the monitoring device may include a memory 1001 and a processor 1002. Wherein:
The memory 1001 is for storing instructions, and the processor 1002 is coupled to the memory 1001, the processor 1002 being configured to perform a method according to any of the embodiments of fig. 1 based on the instructions stored by the memory 1001.
The monitoring device further comprises a communication interface 1003 for information interaction with other devices. Meanwhile, the device further comprises a bus 1004, and the processor 1002, the communication interface 1003, and the memory 1001 perform communication with each other through the bus 1004.
The memory 1001 in fig. 3 may include a high-speed RAM memory, and may further include a non-volatile memory (such as at least one disk memory). Memory 1001 may also be a memory array. The memory 1001 may also be partitioned and the blocks may be combined into virtual volumes according to certain rules.
Further, the processor 1002 in FIG. 3 may be a central processing unit CPU, or may be an Application specific integrated Circuit ASIC (Application SPECIFIC INTEGRATED Circuit), or one or more integrated circuits configured to implement embodiments of the present invention.
It will be apparent to those skilled in the art that embodiments of the present invention may be provided as a method, apparatus, or computer program product. Accordingly, the present invention may take the form of an entirely hardware embodiment, an entirely software embodiment or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable non-transitory storage media (including, but not limited to, disk storage, CD-ROM, optical storage, etc.) having computer-usable program code embodied therein.
The present invention is described with reference to flowchart illustrations and/or block diagrams of methods, apparatus, and computer program products according to embodiments of the invention. It will be understood that each flow and/or block of the flowchart illustrations and/or block diagrams, and combinations of flows and/or blocks in the flowchart illustrations and/or block diagrams, can be implemented by computer program instructions. These computer program instructions may be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, create means for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
The description of the present invention has been presented for purposes of illustration and description, and is not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
Example 1: predicting UHT milk shelf life
UHT pure milk with production date of 2017, 12 months and 4 days is prepared.
The Zeta potential values Z 1、Z2 and Z 3 of the UHT pure milk were measured at t 1、t2 and t 3 days, respectively, and the results were as follows:
| Detection time | Potential difference (mV) |
| t1＝7 | Z1＝-25.18 |
| t2＝17 | Z2＝-23.74 |
| t3＝27 | Z2＝-22.20 |
The above-mentioned materials are mixed
t1＝7、Z1＝-25.18；
t2＝17、Z2＝-23.74；
t3＝27、Z3＝-22.20；
The following formulas are substituted respectively:
|Z|＝k ln(h-t)+c
k=21.45, c= -82.85, h 161 days.
Predicted shelf life was 161 days after the date of manufacture. The shelf life actually noted by the manufacturer is 6 months, which corresponds to 180 days. Thus, the predicted shelf life of the present application differs from the actual shelf life by about 10%.
Example 2: predicting shelf life of UHT sterilized infant liquid milk
Infant liquid milk (UHT sterilized infant liquid milk) having a date of production of 2017, 12 and 4 was prepared.
The Zeta potential values Z 1、Z2 and Z 3 of the above mentioned UHT infant liquid milk were measured at t 1、t2 and t 3 days respectively and the results were as follows:
| Detection time | Potential difference (mV) |
| t1＝102 | Z1＝-21.47 |
| t2＝122 | Z2＝-20.68 |
| t3＝142 | Z2＝-19.81 |
The above-mentioned materials are mixed
t1＝102、Z1＝-21.47；
t2＝122、Z2＝-20.68；
t3＝142、Z3＝-19.81；
The following formulas are substituted respectively:
|Z|＝k ln(h-t)+c
k=8.763, c= -26.260, h=334.
The predicted shelf life is on day 334 after the production date. Manufacturers label 12 months, corresponding to 365 days. Thus, the predicted shelf life of the present application differs from the actual shelf life by about 8.5%.
Example 3: predicting shelf life of UHT sterilized milk-containing beverage
A milk-containing beverage sterilized by UHT was prepared on a date of production of 2017, 1 month and 20 days, wherein the protein content was 2% and the fat content was 2%.
The Zeta potential values Z 1、Z2 and Z 3 of the above UHT milk-containing beverage were measured at t 1、t2 and t 3 days, respectively, and the results were as follows:
| Detection time | Potential difference (mV) |
| t1＝12 | Z1＝-24.88 |
| t2＝27 | Z2＝-24.25 |
| t3＝42 | Z2＝-23.57 |
The above-mentioned materials are mixed
t1＝12、Z1＝-24.88；
t2＝27、Z2＝-24.25；
t3＝42、Z3＝-23.57；
The following formulas are substituted respectively:
|Z|＝k ln(h-t)+c
k=7.913, c= -16.885, h=208 were found.
The predicted shelf life is the 208 th day after the production date. The actual manufacturer labels 8 months, which corresponds to 240 days. Thus, the present application predicts a shelf life deviation from the actual shelf life of about 13.3%.
From examples 1 to 3, the accuracy of the method for predicting the shelf life of the liquid milk obtained by the UHT sterilization method is higher, and the deviation is only 8.5 to 13.3 percent compared with the actual shelf life of the manufacturer standard. Moreover, the method of the embodiment is simple and quick.
Although specific embodiments of the invention have been described in detail, those skilled in the art will appreciate that: many modifications and variations of the details are possible in light of the above teachings, and such variations are within the scope of the invention. The full scope of the invention is given by the appended claims and any equivalents thereof.
Claims (9)
1. A method of predicting shelf life of liquid milk comprising the steps of:
(1) Detecting Zeta potentials Z 1、Z2 and Z 3 of the liquid milk at t 1、t2 and t 3 days from the production day, respectively, wherein t 1、t2 and t 3 are positive integers, and t 1＜t2＜t3;
(2) T=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 is substituted into the following equation,
|Z|＝k ln(h-t)+c
And establishing an equation set, wherein h, k and c are all unknown variables, solving and calculating the value of the unknown variable h, and taking the h day from the production day as the predicted value of the shelf life of the liquid milk.
2. The method according to claim 1, characterized by any one of the following:
■t1＝5～110；
■t2-t1＞10；
■t3-t2＞10。
3. the method according to claim 1, characterized by any one of the following:
■t2-t1＝20～40；
■t3-t2＝20～40。
4. The method of claim 1, which is a method of predicting the shelf life of UHT sterilized liquid milk.
5. A liquid milk shelf life prediction system comprises
The Zeta potential detection module is used for respectively detecting Zeta potentials Z 1、Z2 and Z 3 of the liquid milk at the time t 1、t2 and t 3 from the production day, wherein t 1、t2 and t 3 are positive integers, and t 1＜t2＜t3;
A calculation module for substituting t=t 1、Z＝Z1;t＝t2、Z＝Z2;t＝t3、Z＝Z3 into the following equations,
|Z|＝k ln(h-t)+c
And establishing an equation set, wherein h, k and c are all unknown variables, solving the value of the unknown variable h, and taking the h day from the production day as the predicted value of the shelf life of the liquid milk.
6. The system of claim 5, wherein any of the following:
■t1＝5～110；
■t2-t1＞10；
■t3-t2＞10。
7. the system of claim 5, wherein any of the following:
■t2-t1＝20～40；
■t3-t2＝20～40。
8. The system of claim 5, which is a system that predicts the shelf life of UHT sterilized liquid milk.
9. An apparatus for predicting shelf life of liquid milk, comprising:
A memory; and
A processor coupled to the memory, the processor configured to perform the method of any of claims 1-4 based on instructions stored in the memory.
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