WO2022088777A1 - Set-style flavor yogurt and preparation method therefor - Google Patents

Set-style flavor yogurt and preparation method therefor Download PDF

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Publication number
WO2022088777A1
WO2022088777A1 PCT/CN2021/107508 CN2021107508W WO2022088777A1 WO 2022088777 A1 WO2022088777 A1 WO 2022088777A1 CN 2021107508 W CN2021107508 W CN 2021107508W WO 2022088777 A1 WO2022088777 A1 WO 2022088777A1
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WIPO (PCT)
Prior art keywords
preparation
glue solution
temperature
sugar
milk
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PCT/CN2021/107508
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French (fr)
Chinese (zh)
Inventor
冯东阳
巴根纳
付永刚
樊启程
孙远征
刘华
张凯帅
孟蝶
佟晶晶
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内蒙古伊利实业集团股份有限公司
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Publication of WO2022088777A1 publication Critical patent/WO2022088777A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1315Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/137Thickening substances

Definitions

  • the invention relates to a solidified flavored yogurt and a preparation method thereof, belonging to the technical field of fermented dairy products.
  • the current fermentation process of set yogurt is carried out in the packaging container, so that the finished product retains the curd state due to fermentation.
  • the coagulated yogurt needs to be fermented in a packaging container, so that the coagulated yogurt cannot be fermented and demulsified like agitated yogurt, then pasteurized, and then aseptically filled, so that it can be stored at room temperature for more than 5 months.
  • the solidified yogurt needs to be fermented in the packaging container, which also makes the factory need a large area of constant temperature warehouse when producing solidified yogurt. Compared with the stirred yogurt that can be fermented with a 10T-20T or even larger fermentation tank, it is greatly Increased construction costs.
  • the present invention provides a method for preparing coagulated flavored yogurt that can be fermented in a large tank.
  • the prepared coagulated flavored yogurt can be stored at room temperature, and can solve the technical problem that the current coagulated yogurt needs a large area of constant temperature warehouse and cannot be stored at room temperature during fermentation. .
  • the present invention provides a preparation method of a solidified flavored yogurt, comprising:
  • the base material is homogenized and sterilized and then connected to a fermented strain for fermentation, and the demulsification after fermentation is performed to obtain fermented milk;
  • step (5) utilize two or more mixed raw materials in the described RO permeate and agar, gelatin, carrageenan, xanthan gum, locust bean gum, sugar to prepare the second glue solution, the feed solution obtained in step (4) After heating, it is mixed with the second glue solution;
  • the separated RO permeate is 10-40% of the mass of the raw milk.
  • the temperature of raw milk sterilization is 70-89°C, preferably 70-75°C, and the sterilization time is 10-20s.
  • step (2) includes heating 50%-70% of the fermented milk ingredient amount of the membrane filtration RO retentate to 60-65°C, adding sugar, whey protein powder, cheese powder, mono-, diglycerol Fatty acid ester, mix well, then add remaining RO retentate and mix well.
  • the homogenization temperature is 60-65°C
  • the homogenization pressure is 30/160bar
  • the sterilization temperature is 90-100°C
  • the sterilization time is 2-10min
  • the fermentation temperature is 42- 44°C
  • the fermentation endpoint is 110-125°T
  • the pH is 4.2-4.4.
  • the sterilization temperature is 70-80°C
  • the sterilization time is 20-30s
  • the cooling temperature is 50-70°C
  • the mixing mass ratio is 8:1 (that is, the fermented milk and the first glue The mass ratio when the liquid is mixed)
  • the mixing temperature is 40-50 °C.
  • in-line mixing can be performed using dynamic mixers and static mixers.
  • the preparation method of the first glue solution is as follows: after the RO permeate, which accounts for 91-92% of the first glue solution, is heated to 60-80°C, pectin is added. The first glue solution is sterilized at 110-130 DEG C/40-45s and cooled to 40-45 DEG C.
  • step (5) the heating temperature of the feed liquid is 45-60° C., and the mixing mass ratio is 9:1 (that is, when the feed liquid obtained in step (4) is mixed with the second glue solution after heating mass ratio), and the mixing temperature is 45-60 °C.
  • the preparation method of the second glue solution is: after heating the RO permeate to 60-80° C., adding agar, gelatin, carrageenan, xanthan gum, and locust bean gum Two or more kinds of raw materials and sugar accounting for 4-6% of the amount of the second glue solution are mixed evenly.
  • the second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
  • step (6) the filling temperature is 45-60°C, and the cooling temperature is below 30°C.
  • the weight percentage of the raw materials of the solidified flavored yogurt is: raw milk 86%-91% (preferably 86.2%-91%), sugar 6%-10%, whey protein powder 0.1%-0.5 %, cheese powder 0.2%-0.3%, mono, diglycerol fatty acid ester 0.05%-0.1%, pectin 0.3%-0.6%, agar 0%-0.5%, gelatin 0-0.6%, carrageenan 0%-1 %, xanthan gum 0%-0.1% and locust bean gum 0%-0.2%.
  • the raw milk is raw cow's milk or raw goat's milk.
  • the sugar is one or more of white granulated sugar, fructose syrup, sucrose, glucose, glucose syrup, fructose, maltose, maltose syrup, and lactose.
  • the present invention also provides a solidified flavored yogurt prepared by the preparation method.
  • the preparation method of the present invention has the following beneficial effects:
  • the preparation method of the invention is fermented in a large tank after one batch of ingredients, and does not need to be fermented again in a packaging container, so as to solve the problem that coagulated yogurt needs a large-area constant temperature warehouse; the last two batches + online mixing + pasteurization + sterile heat
  • the filling + online cooling process ensures the solidified state of the product, can be stored at room temperature, and is suitable for industrialized continuous production.
  • the invention adopts membrane concentration to increase the protein content of the raw milk, and improves the nutritional value of the final product, and the protein content of the final product can be higher than 4.0%.
  • This product does not add water during the production process, which maximizes the nutritional value of the product.
  • the raw material of the set-type flavored yogurt includes the following components by mass percentage: raw milk 86%-91%, white sugar 6%-10%, whey protein powder 0.1%-0.5%, cheese powder 0.2%-0.4%, Mono, Diglyceride Fatty Acid Esters 0.05%-0.1%, Pectin 0.3%-0.6%, Agar 0%-0.5%, Gelatin 0-0.6%, Carrageenan 0%-1%, Xanthan Gum 0%-0.1% and Locust Bean Gum 0%-0.2%.
  • the preparation method of the present invention comprises the following steps:
  • the raw milk sterilization conditions are: 75 ⁇ 5°C/15s.
  • RO permeate 15wt%-40wt% of RO permeate is separated out using RO membrane equipment.
  • the purpose of the separation is to increase the protein content of the raw milk and to separate the RO permeate required for the preparation of the first glue solution and the second glue solution.
  • the RO retentate which accounts for 50%-70% of the fermented milk ingredients, is heated to 60-65°C, and some raw materials such as white sugar, whey protein powder, cheese powder, mono- and diglycerol fatty acid esters are added into it. , Using the chemical equipment with mixing, dispersing, shearing and emulsifying effects to make the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well. The use of 50%-70% RO to intercept the liquefied feed is to reduce energy consumption.
  • the base material is homogenized and sterilized and then connected to a fermented strain for fermentation, and the demulsification after fermentation is performed to obtain fermented milk;
  • the base material homogenization and sterilization conditions are:
  • the temperature is 60-65°C, and the homogenization pressure is 30/160bar (the pressure of the homogenizer is divided into two stages, 30bar refers to the secondary pressure, and 160bar refers to the primary pressure).
  • the base is cooled to 42-44°C and pumped into the fermenter.
  • the fermenting strain When fermenting, the fermenting strain is connected, and the added amount of strain is 100-200 DCU/ton, and the fermentation is carried out after stirring for 15-30 minutes, and the fermentation temperature is kept at 42-44°C.
  • the preparation of the first glue solution after the RO permeate, which accounts for 91-92% of the first glue solution, is heated to 60-80° C., pectin and 4.5% of the first glue solution are added. Sugar, and mix evenly, the first glue solution is sterilized at 110-130°C/40-45s, and cooled to 40-45°C.
  • the first glue is used to prevent protein denaturation during sterilization and to provide product shelf life stability.
  • the mixing mass ratio of fermented milk base material A and the first glue liquid is 8:1.
  • Sterilization parameters 70-80°C/20-30s.
  • Cooling temperature 20-30°C.
  • the preparation method of the second glue solution is as follows: after the RO permeate is heated to 60-80° C., adding two or more raw materials of agar, gelatin, carrageenan, xanthan gum and locust bean gum and white granulated sugar, which accounts for 5% of the ingredient amount of the second glue solution, and mix them evenly to obtain the second glue solution.
  • the second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
  • the second glue is used to provide the set texture of the product.
  • Fermented milk base material B heating temperature 45-60 °C.
  • the mixing mass ratio of the fermented milk base material B and the second glue liquid is 9:1.
  • the filling temperature is 45-60°C.
  • the product formula ratio of the coagulated flavored yogurt is: 90% raw milk, 8% white sugar, 0.3% whey protein powder, 0.2% cheese powder, 0.1% mono- and diglycerol fatty acids ester, 0.4% pectin, 0.4% agar, 0.4% gelatin, 0.2% carrageenan.
  • the production process of the solidified flavored yogurt product is as follows:
  • the RO entrapment liquid which accounts for 60% of the fermented milk ingredients, is heated to 63°C, and some raw materials such as white granulated sugar, whey protein powder, cheese powder, mono- and di-glycerol fatty acid esters are added into it, and the mixture is mixed and dispersed. , shearing, emulsifying effect of the material equipment, so that the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well.
  • homogenization temperature 63°C, homogenization pressure 30/160bar.
  • the base was cooled to 43°C and pumped into the fermenter.
  • the fermenting strains When fermenting, the fermenting strains are connected, and the amount of strains added is 100-200 DCU/ton. After stirring for 20 minutes, static fermentation is performed, and the fermentation temperature is kept at 43 °C.
  • Preparation of the first glue solution after heating the RO permeate, which accounts for 91-92% of the first glue solution, to 60-80 °C, add pectin and white granulated sugar, which accounts for 4.5% of the first glue solution. , and mixed evenly to obtain the first glue solution, then the first glue solution is subjected to sterilization treatment at 110°C-130°C/40-45s, and cooled to 40-45°C.
  • Sterilization parameters 70-80°C/20-30s.
  • Cooling temperature 20-30°C.
  • Preparation of the second glue solution after heating the RO permeate that accounts for 83-84% of the second glue solution to 70° C., add agar, gelatin, carrageenan and 5% of the second glue solution. White granulated sugar, and mix well to obtain the second glue.
  • the second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
  • the mixing ratio of the fermented milk base material B and the second glue liquid is 9:1.
  • Packaging sleeve labeling, scooping, covering, packing, coding, etc.
  • the product formula ratio of the solidified flavored yogurt is: raw milk 89.9%, white sugar 8%, whey protein powder 0.3%, cheese powder 0.2%, mono- and diglycerol fatty acid ester 0.05%, pectin 0.5% %, agar 0.1%, carrageenan 0.9%, locust bean gum 0.05%.
  • the production process of the solidified flavored yogurt product is as follows:
  • the RO entrapment liquid which accounts for 60% of the fermented milk ingredients, is heated to 63 ° C, and some raw materials such as white granulated sugar, whey protein powder, cheese powder, mono- and di-glycerol fatty acid esters are added into it, and the mixture is mixed and dispersed. , shearing, emulsifying effect of the material equipment, so that the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well.
  • homogenization temperature 63°C, homogenization pressure 30/160bar.
  • the base was cooled to 43°C and pumped into the fermenter.
  • fermenting strains were added, and the added amount of strains was 200 DCU/ton. After stirring for 20 minutes, static fermentation was performed, and the fermentation temperature was maintained at 43 °C.
  • Preparation of the first glue solution after heating the RO permeate that accounts for 91-92% of the first glue solution to 60-80° C., add pectin and white granulated sugar accounting for 4.5% of the first glue solution , and mixed evenly to obtain the first glue solution, and then the first glue solution is subjected to sterilization treatment at 110°C-130°C/40-45s, and cooled to 40-45°C.
  • Cooling temperature 20-30°C.
  • Preparation of the second glue solution after heating the RO permeate that accounts for 85% of the second glue solution to 70°C, add agar, gelatin, carrageenan and white granulated sugar accounting for 5% of the second glue solution , and mixed evenly to obtain the second glue.
  • the second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
  • the mixing ratio of fermented milk base material B and the second glue is 9:1;
  • Packaging sleeve labeling, scooping, covering, packing, coding, etc.
  • feed liquid B (6) adding 15 kilograms of white granulated sugar, 1.1 kilograms of agar, 0.7 kilograms of gelatin and 0.8 kilograms of konjac gum after heating the feed liquid A to 60-65°C after fermentation, and stirring and dissolving to obtain feed liquid B;
  • Product hardness reflects the force of the teeth to squeeze the sample. According to the sensory test, the yogurt product has a solidified state when the hardness reaches more than 90g. If the hardness is too high, the taste is not good. If the hardness is too low, the texture state of the product is easy to be stored and sold. changes happened.
  • the suitable hardness of solidified yogurt is 100-200g.
  • Example 1, Example 2, and Comparative Example 1 all satisfy the texture state of solidified yogurt.
  • Comparative Example 1 is soft, and Example 1 and Example 2 are more suitable for softness and hardness.
  • Example 1-2 and Comparative Example 1 Take the products of Example 1-2 and Comparative Example 1 as taste test samples, and carry out a taste and flavor evaluation experiment: a total of 200 people (100 men and women aged 18-28), respectively The product and the product of Comparative Example 1 were tasted, and an anonymous scoring system was used to score the overall preference of the product.
  • the scoring criteria are as follows:
  • the sample whose texture state is broken is the sample whose solidification state of the product is changed due to the action of physical external force.
  • Example 1 and Example 2 the breakage rate of Example 1 and Example 2 is significantly lower than that of Comparative Example 1, indicating that the hardness of Example 1 and Example 2 is more suitable for transportation at room temperature, while the hardness of the product of Comparative Example 1 is softer, and when transported at room temperature The texture state of the product is prone to change.
  • Example 1 No obvious whey separation No obvious whey separation
  • Example 2 No obvious whey separation No obvious whey separation Comparative Example 1 A small amount of whey dehydrated more whey
  • Example 1 No obvious whey separation A small amount of whey dehydrated Example 2 No obvious whey separation A small amount of whey dehydrated Comparative Example 1 more whey water more whey
  • microorganisms of the solidified flavored yogurt prepared in the above-described embodiment are detected immediately, and the detection results are as follows:
  • the solidified flavored yogurt prepared in the above embodiment was stored for 6 months under normal temperature (25° C.) conditions, and the microorganisms were detected.
  • the results are as shown in the following table:

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Abstract

A preparation method for set-style flavor yogurt, comprising: (1) sterilizing raw milk and then separating same by a RO membrane filtration device to obtain a RO trapped fluid and a RO penetrating fluid; (2) uniformly mixing a portion of the RO trapped fluid with sugar, whey protein powder, cheese powder, and mono- and di-glycerol fatty acid esters to obtain a base material; (3) homogenizing and sterilizing the base material, then inoculating same into a fermentation strain for fermentation, and performing demulsification to obtain fermented milk; (4) mixing the fermented milk with a first glue solution prepared by using the RO penetrating fluid, pectin, and sugar, and performing sterilization and cooling; (5) preparing a second glue solution by mixing the RO penetrating fluid with two or more raw materials of agar, gelatin, carrageenan, xanthan gum, locust bean gum and sugar, heating feed liquid obtained in step (4), and then mixing the feed liquid with the second glue solution; and (6) performing online cooling after aseptic hot filling to obtain the set-style flavor yogurt. Also provided is the set-style flavor yogurt prepared by using the preparation method.

Description

一种凝固型风味酸奶及其制备方法A kind of solidified flavored yogurt and preparation method thereof 技术领域technical field
本发明涉及一种凝固型风味酸奶及其制备方法,属于发酵乳制品技术领域。The invention relates to a solidified flavored yogurt and a preparation method thereof, belonging to the technical field of fermented dairy products.
背景技术Background technique
目前凝固型酸奶的发酵过程是在包装容器中进行的,从而使成品因发酵而保留了凝乳状态。凝固型酸奶需要在包装容器中发酵的工艺使凝固型酸奶不能像搅拌型酸奶一样发酵破乳后进行巴氏杀菌,再进行无菌灌装,从而达到常温5个月以上的保藏。The current fermentation process of set yogurt is carried out in the packaging container, so that the finished product retains the curd state due to fermentation. The coagulated yogurt needs to be fermented in a packaging container, so that the coagulated yogurt cannot be fermented and demulsified like agitated yogurt, then pasteurized, and then aseptically filled, so that it can be stored at room temperature for more than 5 months.
凝固型酸奶的工艺限制,使目前凝固型酸奶均需要低温保藏、冷链运输、冷链销售,且保质期一般在28天左右。运输及销售成本较高。Due to the technological limitations of solidified yogurt, the current solidified yogurt requires low temperature storage, cold chain transportation, and cold chain sales, and the shelf life is generally about 28 days. Shipping and sales costs are high.
此外,凝固型酸奶需要在包装容器中进行发酵,也使工厂在生产凝固型酸奶时,需要大面积的恒温库房,相比可使用10T-20T乃至更大发酵罐进行发酵的搅拌型酸奶,大大增加了建厂成本。In addition, the solidified yogurt needs to be fermented in the packaging container, which also makes the factory need a large area of constant temperature warehouse when producing solidified yogurt. Compared with the stirred yogurt that can be fermented with a 10T-20T or even larger fermentation tank, it is greatly Increased construction costs.
发明内容SUMMARY OF THE INVENTION
本发明提供一种可采用大罐进行发酵的凝固型风味酸奶的制备方法,制备的凝固型风味酸奶可以常温保藏,可以解决目前凝固型酸奶发酵时需要大面积恒温库房、不能常温存放的技术难题。The present invention provides a method for preparing coagulated flavored yogurt that can be fermented in a large tank. The prepared coagulated flavored yogurt can be stored at room temperature, and can solve the technical problem that the current coagulated yogurt needs a large area of constant temperature warehouse and cannot be stored at room temperature during fermentation. .
为了达到上述目的,本发明提供一种凝固型风味酸奶的制备方法,包括:In order to achieve the above object, the present invention provides a preparation method of a solidified flavored yogurt, comprising:
(1)将原料乳杀菌后经过RO膜过滤设备分离得到RO截留液和RO渗透液;(1) after the raw milk is sterilized, the RO retentate and the RO permeate are obtained through RO membrane filtration equipment separation;
(2)将部分所述RO截留液与糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯混合均匀,得到基料;(2) mixing part of the RO retentate with sugar, whey protein powder, cheese powder, mono- and diglycerol fatty acid esters to obtain base material;
(3)将所述基料均质、杀菌后接入发酵菌种进行发酵,发酵后破乳得到发酵乳;(3) the base material is homogenized and sterilized and then connected to a fermented strain for fermentation, and the demulsification after fermentation is performed to obtain fermented milk;
(4)将所述发酵乳与利用所述RO渗透液、果胶和糖制备的第一胶液混合,均质,杀菌,冷却;(4) mixing the fermented milk with the first glue prepared by utilizing the RO permeate, pectin and sugar, homogenizing, sterilizing, and cooling;
(5)利用所述RO渗透液与琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶、糖中的两种或多种混合原料制备第二胶液,将步骤(4)所得的料液加热后与所述第二胶液混合;(5) utilize two or more mixed raw materials in the described RO permeate and agar, gelatin, carrageenan, xanthan gum, locust bean gum, sugar to prepare the second glue solution, the feed solution obtained in step (4) After heating, it is mixed with the second glue solution;
(6)无菌热灌装后进行在线冷却,得到凝固型风味酸奶。(6) on-line cooling after aseptic hot filling to obtain solidified flavored yogurt.
在一些实施方案中,步骤(1)中,分离出的所述RO渗透液为所述原料乳质量的 10-40%。In some embodiments, in step (1), the separated RO permeate is 10-40% of the mass of the raw milk.
在一些实施方案中,步骤(1)中,原料乳杀菌的温度为70-89℃,优选为70-75℃,杀菌时间为10-20s。In some embodiments, in step (1), the temperature of raw milk sterilization is 70-89°C, preferably 70-75°C, and the sterilization time is 10-20s.
在一些实施方案中,步骤(2)包括将占发酵乳配料量50%-70%的膜过滤RO截留液加热至60-65℃,加入糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯,混合均匀,然后加入剩余的RO截留液,并混合均匀。In some embodiments, step (2) includes heating 50%-70% of the fermented milk ingredient amount of the membrane filtration RO retentate to 60-65°C, adding sugar, whey protein powder, cheese powder, mono-, diglycerol Fatty acid ester, mix well, then add remaining RO retentate and mix well.
在一些实施方案中,步骤(3)中,均质的温度为60-65℃,均质压力为30/160bar,杀菌温度为90-100℃,杀菌时间为2-10min,发酵温度为42-44℃,发酵终点为110-125°T,pH为4.2-4.4。In some embodiments, in step (3), the homogenization temperature is 60-65°C, the homogenization pressure is 30/160bar, the sterilization temperature is 90-100°C, the sterilization time is 2-10min, and the fermentation temperature is 42- 44°C, the fermentation endpoint is 110-125°T, and the pH is 4.2-4.4.
在一些实施方案中,步骤(4)中,杀菌温度为70-80℃,杀菌时间为20-30s,冷却温度为50-70℃,混合质量比为8:1(即发酵乳与第一胶液进行混合时的质量比),混合温度为40-50℃。优选地,可采用动态混合器及静态混合器进行在线混合。In some embodiments, in step (4), the sterilization temperature is 70-80°C, the sterilization time is 20-30s, the cooling temperature is 50-70°C, and the mixing mass ratio is 8:1 (that is, the fermented milk and the first glue The mass ratio when the liquid is mixed), and the mixing temperature is 40-50 °C. Preferably, in-line mixing can be performed using dynamic mixers and static mixers.
在一些实施方案中,步骤(4)中,所述第一胶液的制备方法为:将占第一胶液配料量91-92%的RO渗透液加热至60-80℃后,加入果胶和占第一胶液配料量3-5%的糖,并混合均匀,第一胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到40-45℃。In some embodiments, in step (4), the preparation method of the first glue solution is as follows: after the RO permeate, which accounts for 91-92% of the first glue solution, is heated to 60-80°C, pectin is added. The first glue solution is sterilized at 110-130 DEG C/40-45s and cooled to 40-45 DEG C.
在一些实施方案中,步骤(5)中,料液加热温度为45-60℃,混合质量比为9:1(即步骤(4)所得的料液在加热后与第二胶液进行混合时的质量比),混合温度为45-60℃。In some embodiments, in step (5), the heating temperature of the feed liquid is 45-60° C., and the mixing mass ratio is 9:1 (that is, when the feed liquid obtained in step (4) is mixed with the second glue solution after heating mass ratio), and the mixing temperature is 45-60 °C.
在一些实施方案中,步骤(5)中,所述第二胶液的制备方法为:将RO渗透液加热至60-80℃后,加入琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶中的两种或多种原料及占第二胶液配料量4-6%的糖,并混合均匀。第二胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到50-65℃。In some embodiments, in step (5), the preparation method of the second glue solution is: after heating the RO permeate to 60-80° C., adding agar, gelatin, carrageenan, xanthan gum, and locust bean gum Two or more kinds of raw materials and sugar accounting for 4-6% of the amount of the second glue solution are mixed evenly. The second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
在一些实施方案中,步骤(6)中,灌装温度为45-60℃,冷却温度为30℃以下。In some embodiments, in step (6), the filling temperature is 45-60°C, and the cooling temperature is below 30°C.
在一些实施方案中,所述凝固型风味酸奶的原料的重量百分比为:原料乳86%-91%(优选86.2%-91%)、糖6%-10%、乳清蛋白粉0.1%-0.5%、芝士粉0.2%-0.3%、单,双甘油脂肪酸酯0.05%-0.1%、果胶0.3%-0.6%、琼脂0%-0.5%、明胶0-0.6%、卡拉胶0%-1%、黄原胶0%-0.1%和刺槐豆胶0%-0.2%。In some embodiments, the weight percentage of the raw materials of the solidified flavored yogurt is: raw milk 86%-91% (preferably 86.2%-91%), sugar 6%-10%, whey protein powder 0.1%-0.5 %, cheese powder 0.2%-0.3%, mono, diglycerol fatty acid ester 0.05%-0.1%, pectin 0.3%-0.6%, agar 0%-0.5%, gelatin 0-0.6%, carrageenan 0%-1 %, xanthan gum 0%-0.1% and locust bean gum 0%-0.2%.
在一些实施方案中,所述原料乳为生牛乳或生羊乳。所述糖为白砂糖、果葡糖浆、蔗糖、葡萄糖、葡萄糖浆、果糖、麦芽糖、麦芽糖浆、乳糖中的一种或多种。In some embodiments, the raw milk is raw cow's milk or raw goat's milk. The sugar is one or more of white granulated sugar, fructose syrup, sucrose, glucose, glucose syrup, fructose, maltose, maltose syrup, and lactose.
本发明还提供一种利用所述制备方法制备的凝固型风味酸奶。The present invention also provides a solidified flavored yogurt prepared by the preparation method.
与现有技术相比,本发明的制备方法具有以下有益效果:Compared with the prior art, the preparation method of the present invention has the following beneficial effects:
本发明的制备方法于一次配料后在大罐中发酵,无需在包装容器中再发酵,解决凝固型酸奶需要大面积恒温库房的问题;最后两次配料+在线混合+巴氏杀菌+无菌热灌装+在线冷却工艺保证产品凝固状态、可常温存放、适宜工业化连续生产。The preparation method of the invention is fermented in a large tank after one batch of ingredients, and does not need to be fermented again in a packaging container, so as to solve the problem that coagulated yogurt needs a large-area constant temperature warehouse; the last two batches + online mixing + pasteurization + sterile heat The filling + online cooling process ensures the solidified state of the product, can be stored at room temperature, and is suitable for industrialized continuous production.
本发明采用膜浓缩提高原料奶的蛋白质含量,提高了终产品的营养价值,终产品蛋白质含量可高于4.0%。The invention adopts membrane concentration to increase the protein content of the raw milk, and improves the nutritional value of the final product, and the protein content of the final product can be higher than 4.0%.
本产品在生产过程中不会添加水,最大限度保证了产品的营养价值。This product does not add water during the production process, which maximizes the nutritional value of the product.
具体实施方式Detailed ways
为使本发明的目的、技术方案和优点更加清楚明白,以下结合具体实施例对本发明作进一步的详细说明。In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to specific embodiments.
本发明提供了一种凝固型风味酸奶的制备方法,可以解决目前凝固型酸奶发酵时需要大面积恒温库房、不能常温存放的技术难题。在一些是实施方案中,凝固型风味酸奶的原料包括下述质量百分比的组分:原料乳86%-91%、白砂糖6%-10%、乳清蛋白粉0.1%-0.5%、芝士粉0.2%-0.4%、单,双甘油脂肪酸酯0.05%-0.1%、果胶0.3%-0.6%、琼脂0%-0.5%、明胶0-0.6%、卡拉胶0%-1%、黄原胶0%-0.1%和刺槐豆胶0%-0.2%。本发明所述的制备方法包括如下步骤:The invention provides a preparation method of solidified flavored yogurt, which can solve the technical problem that the current solidified yogurt needs a large-area constant temperature warehouse during fermentation and cannot be stored at normal temperature. In some embodiments, the raw material of the set-type flavored yogurt includes the following components by mass percentage: raw milk 86%-91%, white sugar 6%-10%, whey protein powder 0.1%-0.5%, cheese powder 0.2%-0.4%, Mono, Diglyceride Fatty Acid Esters 0.05%-0.1%, Pectin 0.3%-0.6%, Agar 0%-0.5%, Gelatin 0-0.6%, Carrageenan 0%-1%, Xanthan Gum 0%-0.1% and Locust Bean Gum 0%-0.2%. The preparation method of the present invention comprises the following steps:
(1)将原料乳杀菌后经过反渗透装置得到RO截留液和RO渗透液;(1) obtain RO retentate and RO permeate through reverse osmosis device after raw material milk is sterilized;
该步骤中,原料乳杀菌的条件为:75±5℃/15s。In this step, the raw milk sterilization conditions are: 75±5°C/15s.
使用RO膜设备分离出15wt%-40wt%的RO渗透液。分离的目的为提高原料奶的蛋白质含量及分离出制备第一胶液和第二胶液所需的RO渗透液。15wt%-40wt% of RO permeate is separated out using RO membrane equipment. The purpose of the separation is to increase the protein content of the raw milk and to separate the RO permeate required for the preparation of the first glue solution and the second glue solution.
(2)将部分所述RO截留液与部分白砂糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯等原料混合均匀,得到基料;(2) mixing part of the RO retentate with part of white granulated sugar, whey protein powder, cheese powder, mono-, diglyceride fatty acid esters and other raw materials to obtain base material;
该步骤中,将占发酵乳配料量50%-70%的RO截留液加热至60-65℃,将部分白砂糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯等原料加入其中,采用具有混合、分散、剪切、乳化效果的化料设备,使料液成为均匀、分散混合物。将剩余的RO截留液打入其中混合均匀。使用50%-70%的RO截留液化料是为了减少能源的消耗。In this step, the RO retentate, which accounts for 50%-70% of the fermented milk ingredients, is heated to 60-65°C, and some raw materials such as white sugar, whey protein powder, cheese powder, mono- and diglycerol fatty acid esters are added into it. , Using the chemical equipment with mixing, dispersing, shearing and emulsifying effects to make the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well. The use of 50%-70% RO to intercept the liquefied feed is to reduce energy consumption.
(3)将所述基料均质、杀菌后接入发酵菌种进行发酵,发酵后破乳得到发酵乳;(3) the base material is homogenized and sterilized and then connected to a fermented strain for fermentation, and the demulsification after fermentation is performed to obtain fermented milk;
该步骤中,基料均质、杀菌条件为:In this step, the base material homogenization and sterilization conditions are:
均质:温度60-65℃,均质压力30/160bar(均质机压力分为两级,30bar指的二级压力,160bar指的一级压力)。Homogenization: The temperature is 60-65°C, and the homogenization pressure is 30/160bar (the pressure of the homogenizer is divided into two stages, 30bar refers to the secondary pressure, and 160bar refers to the primary pressure).
杀菌:95℃/5min。Sterilization: 95℃/5min.
基料冷却至42-44℃后泵入发酵罐。The base is cooled to 42-44°C and pumped into the fermenter.
发酵时,接入发酵菌种,菌种添加量100-200DCU/吨,搅拌15-30min后进行静置发酵,发酵温度保持在42-44℃。When fermenting, the fermenting strain is connected, and the added amount of strain is 100-200 DCU/ton, and the fermentation is carried out after stirring for 15-30 minutes, and the fermentation temperature is kept at 42-44°C.
待料液酸度110-125°T,pH4.2-4.4进行破乳,得到发酵乳基料A。When the acidity of the feed liquid is 110-125°T and the pH is 4.2-4.4, demulsification is carried out to obtain the fermented milk base material A.
(4)将所述发酵乳基料A与利用所述RO渗透液、果胶和部分白砂糖制备的第一胶液进行在线混合,均质,杀菌,冷却,得到发酵乳基料B;(4) on-line mixing of described fermented milk base material A with the first glue prepared by utilizing described RO permeate, pectin and part of white granulated sugar, homogenization, sterilization, and cooling to obtain fermented milk base material B;
该步骤中,第一胶液的制备:将占第一胶液配料量91-92%的RO渗透液加热至60-80℃后,加入果胶和占第一胶液配料量4.5%的白砂糖,并混合均匀,第一胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到40-45℃。第一胶液用于防止蛋白在杀菌过程中变性,并提供产品货架期稳定性。In this step, the preparation of the first glue solution: after the RO permeate, which accounts for 91-92% of the first glue solution, is heated to 60-80° C., pectin and 4.5% of the first glue solution are added. Sugar, and mix evenly, the first glue solution is sterilized at 110-130°C/40-45s, and cooled to 40-45°C. The first glue is used to prevent protein denaturation during sterilization and to provide product shelf life stability.
发酵乳基料A与第一胶液混合质量比为8:1。The mixing mass ratio of fermented milk base material A and the first glue liquid is 8:1.
混合温度:40-45℃。Mixing temperature: 40-45℃.
均质:20/100±5bar。Homogenization: 20/100±5bar.
杀菌参数:70-80℃/20-30s。Sterilization parameters: 70-80℃/20-30s.
冷却温度:20-30℃。Cooling temperature: 20-30℃.
(5)将上述发酵乳基料B加热后与利用所述RO渗透液、琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶、部分白砂糖中的两种或多种混合原料制备的第二胶液混合、均质;(5) after the above-mentioned fermented milk base material B is heated and utilize two or more mixed raw materials in the described RO permeate, agar, gelatin, carrageenan, xanthan gum, locust bean gum, part white granulated sugar to prepare the first The two glues are mixed and homogenized;
该步骤中,所述第二胶液的制备方法为:将RO渗透液加热至60-80℃后,加入琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶中的两种或多种原料和占第二胶液配料量5%的白砂糖,并混合均匀,得到第二胶液。第二胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到50-65℃。第二胶液用于提供产品凝固型质构。In this step, the preparation method of the second glue solution is as follows: after the RO permeate is heated to 60-80° C., adding two or more raw materials of agar, gelatin, carrageenan, xanthan gum and locust bean gum and white granulated sugar, which accounts for 5% of the ingredient amount of the second glue solution, and mix them evenly to obtain the second glue solution. The second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C. The second glue is used to provide the set texture of the product.
发酵乳基料B加热温度:45-60℃。Fermented milk base material B heating temperature: 45-60 ℃.
发酵乳基料B与第二胶液的混合质量比为9:1。The mixing mass ratio of the fermented milk base material B and the second glue liquid is 9:1.
均质参数:20/100±5bar。Homogenization parameters: 20/100±5bar.
(6)无菌热灌装后进行在线冷却,得到凝固型风味酸奶。(6) on-line cooling after aseptic hot filling to obtain solidified flavored yogurt.
该步骤中,灌装温度45-60℃。In this step, the filling temperature is 45-60°C.
无菌冷却至30℃以下。Aseptically cool to below 30°C.
包装、出厂。Packed and shipped from the factory.
实施例1Example 1
该实施例中,凝固型风味酸奶的产品配方比例为:90%的生牛乳、8%的白砂糖、0.3% 的乳清蛋白粉、0.2%的芝士粉、0.1%的单,双甘油脂肪酸酯、0.4%的果胶、0.4%的琼脂、0.4%的明胶、0.2%的卡拉胶。In this embodiment, the product formula ratio of the coagulated flavored yogurt is: 90% raw milk, 8% white sugar, 0.3% whey protein powder, 0.2% cheese powder, 0.1% mono- and diglycerol fatty acids ester, 0.4% pectin, 0.4% agar, 0.4% gelatin, 0.2% carrageenan.
凝固型风味酸奶产品的生产工艺如下:The production process of the solidified flavored yogurt product is as follows:
(1)原奶预杀菌:75±5℃/15s。(1) Pre-sterilization of raw milk: 75±5℃/15s.
(2)使用RO膜设备分离出占预杀菌牛奶量25wt%的RO渗透液。(2) Using RO membrane equipment to separate the RO permeate which accounts for 25wt% of the pre-sterilized milk.
(3)将占发酵乳配料量60%的RO截留液加热至63℃,将部分白砂糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯等原料加入其中,采用具有混合、分散、剪切、乳化效果的化料设备,使料液成为均匀、分散混合物。将剩余的RO截留液打入其中混合均匀。(3) The RO entrapment liquid, which accounts for 60% of the fermented milk ingredients, is heated to 63°C, and some raw materials such as white granulated sugar, whey protein powder, cheese powder, mono- and di-glycerol fatty acid esters are added into it, and the mixture is mixed and dispersed. , shearing, emulsifying effect of the material equipment, so that the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well.
均质:温度63℃,均质压力30/160bar。Homogenization: temperature 63℃, homogenization pressure 30/160bar.
杀菌:95℃/5min。Sterilization: 95℃/5min.
基料冷却至43℃后泵入发酵罐。The base was cooled to 43°C and pumped into the fermenter.
发酵时接入发酵菌种,菌种添加量100-200DCU/吨,搅拌20min后进行静置发酵,发酵温度保持在43℃。When fermenting, the fermenting strains are connected, and the amount of strains added is 100-200 DCU/ton. After stirring for 20 minutes, static fermentation is performed, and the fermentation temperature is kept at 43 °C.
待料液酸度110°T,pH4.4时进行破乳,得到发酵乳基料A。When the acidity of the feed liquid is 110°T and pH 4.4, demulsification is carried out to obtain fermented milk base material A.
(4)第一胶液的制备:将占第一胶液配料量91-92%的RO渗透液加热至60-80℃后,加入果胶和占第一胶液配料量4.5%的白砂糖,并混合均匀,得到第一胶液,然后对第一胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到40-45℃。(4) Preparation of the first glue solution: after heating the RO permeate, which accounts for 91-92% of the first glue solution, to 60-80 °C, add pectin and white granulated sugar, which accounts for 4.5% of the first glue solution. , and mixed evenly to obtain the first glue solution, then the first glue solution is subjected to sterilization treatment at 110°C-130°C/40-45s, and cooled to 40-45°C.
(5)发酵乳基料A与第一胶液进行定量混合,得到发酵乳基料B。(5) Quantitatively mixing the fermented milk base A and the first glue solution to obtain the fermented milk base B.
混合比例:8:1。Mixing ratio: 8:1.
混合温度:40-45℃。Mixing temperature: 40-45℃.
均质:20/100±5bar。Homogenization: 20/100±5bar.
(6)发酵乳基料B进行杀菌(6) Sterilize the fermented milk base material B
杀菌参数:70-80℃/20-30s。Sterilization parameters: 70-80℃/20-30s.
冷却温度:20-30℃。Cooling temperature: 20-30℃.
(7)第二胶液的制备:将占第二胶液配料量83-84%的RO渗透液加热至70℃后,加入琼脂、明胶、卡拉胶和占第二胶液配料量5%的白砂糖,并混合均匀,得到第二胶液。第二胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到50-65℃。(7) Preparation of the second glue solution: after heating the RO permeate that accounts for 83-84% of the second glue solution to 70° C., add agar, gelatin, carrageenan and 5% of the second glue solution. White granulated sugar, and mix well to obtain the second glue. The second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
(8)发酵乳基料B加热至45-60℃后与第二胶液进行定量混合、均质。(8) After heating the fermented milk base material B to 45-60° C., quantitatively mix and homogenize it with the second glue solution.
发酵乳基料B与第二胶液的混合比例为9:1。The mixing ratio of the fermented milk base material B and the second glue liquid is 9:1.
均质参数:20/100±5bar。Homogenization parameters: 20/100±5bar.
(9)灌装:灌装温度45-60℃。(9) Filling: Filling temperature is 45-60℃.
(10)冷却凝固:冷却至30℃以下(凝固需要30min)。(10) Cooling and solidification: cooling to below 30°C (30min is required for solidification).
(11)包装:套标、加勺、套盖、装箱、喷码等。(11) Packaging: sleeve labeling, scooping, covering, packing, coding, etc.
(12)出厂。(12) Factory.
实施例2Example 2
该实施例中,凝固型风味酸奶的产品配方比例为:生牛乳89.9%、白砂糖8%、乳清蛋白粉0.3%、芝士粉0.2%、单,双甘油脂肪酸酯0.05%、果胶0.5%、琼脂0.1%、卡拉胶0.9%、刺槐豆胶0.05%。In this embodiment, the product formula ratio of the solidified flavored yogurt is: raw milk 89.9%, white sugar 8%, whey protein powder 0.3%, cheese powder 0.2%, mono- and diglycerol fatty acid ester 0.05%, pectin 0.5% %, agar 0.1%, carrageenan 0.9%, locust bean gum 0.05%.
凝固型风味酸奶产品的生产工艺如下:The production process of the solidified flavored yogurt product is as follows:
(1)原奶预杀菌:75±5℃/15s。(1) Pre-sterilization of raw milk: 75±5℃/15s.
(2)使用RO膜设备分离出占预杀菌牛奶量30wt%的RO渗透液。(2) Using RO membrane equipment to separate the RO permeate which accounts for 30wt% of the pre-sterilized milk.
(3)将占发酵乳配料量60%的RO截留液加热至63℃,将部分白砂糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯等原料加入其中,采用具有混合、分散、剪切、乳化效果的化料设备,使料液成为均匀、分散混合物。将剩余的RO截留液打入其中混合均匀。(3) The RO entrapment liquid, which accounts for 60% of the fermented milk ingredients, is heated to 63 ° C, and some raw materials such as white granulated sugar, whey protein powder, cheese powder, mono- and di-glycerol fatty acid esters are added into it, and the mixture is mixed and dispersed. , shearing, emulsifying effect of the material equipment, so that the material liquid into a uniform and dispersed mixture. Pour the remaining RO retentate into it and mix well.
均质:温度63℃,均质压力30/160bar。Homogenization: temperature 63℃, homogenization pressure 30/160bar.
杀菌:95℃/5min。Sterilization: 95℃/5min.
基料冷却至43℃后泵入发酵罐。The base was cooled to 43°C and pumped into the fermenter.
发酵时接入发酵菌种,菌种添加量200DCU/吨,搅拌20min后进行静置发酵,发酵温度保持在43℃。During fermentation, fermenting strains were added, and the added amount of strains was 200 DCU/ton. After stirring for 20 minutes, static fermentation was performed, and the fermentation temperature was maintained at 43 °C.
待料液酸度110°T,pH4.4以下时进行破乳,得到发酵乳基料A。When the acidity of the feed liquid is 110°T and pH is below 4.4, demulsification is carried out to obtain fermented milk base material A.
(4)第一胶液的制备:将占第一胶液配料量91-92%的RO渗透液加热至60-80℃后,加入果胶和占第一胶液配料量4.5%的白砂糖,并混合均匀,得到第一胶液,然后对第一胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到40-45℃。(4) Preparation of the first glue solution: after heating the RO permeate that accounts for 91-92% of the first glue solution to 60-80° C., add pectin and white granulated sugar accounting for 4.5% of the first glue solution , and mixed evenly to obtain the first glue solution, and then the first glue solution is subjected to sterilization treatment at 110°C-130°C/40-45s, and cooled to 40-45°C.
(5)发酵乳基料A与第一胶液进行定量混合,得到发酵乳基料B。(5) Quantitatively mixing the fermented milk base material A and the first glue solution to obtain the fermented milk base material B.
混合比例:8:1;Mixing ratio: 8:1;
混合温度:40-45℃;Mixing temperature: 40-45℃;
均质:20/100±5bar。Homogenization: 20/100±5bar.
(6)发酵乳基料B进行杀菌(6) Sterilize the fermented milk base material B
杀菌参数:70-80℃/20-30s;Sterilization parameters: 70-80℃/20-30s;
冷却温度:20-30℃。Cooling temperature: 20-30℃.
(7)第二胶液的制备:将占第二胶液配料量85%的RO渗透液加热至70℃后,加入琼脂、明胶、卡拉胶和占第二胶液配料量5%的白砂糖,并混合均匀,得到第二胶液。第二胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到50-65℃。(7) Preparation of the second glue solution: after heating the RO permeate that accounts for 85% of the second glue solution to 70°C, add agar, gelatin, carrageenan and white granulated sugar accounting for 5% of the second glue solution , and mixed evenly to obtain the second glue. The second glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 50-65°C.
(8)发酵乳基料B加热至45-60℃后与第二胶液进行定量混合、均质。(8) After heating the fermented milk base material B to 45-60° C., quantitatively mix and homogenize it with the second glue solution.
发酵乳基料B与第二胶液的混合比例为9:1;The mixing ratio of fermented milk base material B and the second glue is 9:1;
均质参数:20/100±5barHomogenization parameters: 20/100±5bar
(9)灌装:灌装温度45-60℃。(9) Filling: Filling temperature is 45-60℃.
(10)冷却凝固:冷却至30℃以下(凝固需要1h)。(10) Cooling and solidification: cooling to below 30°C (1h required for solidification).
(11)包装:套标、加勺、套盖、装箱、喷码等。(11) Packaging: sleeve labeling, scooping, covering, packing, coding, etc.
(12)出厂。(12) Factory.
对比例1Comparative Example 1
(1)将1000千克牛奶杀菌,杀菌温度130℃,时长3秒;(1) Sterilize 1000 kg of milk at a sterilization temperature of 130°C for 3 seconds;
(2)将55千克的白砂糖、9.2千克的羟丙基二淀粉磷酸酯和0.1千克的果胶混合;混合后与0.2千克稀奶油、3千克乳清蛋白粉一起加入杀菌后的牛奶中混合溶解,得到料液A;(2) Mix 55 kg of white sugar, 9.2 kg of hydroxypropyl distarch phosphate and 0.1 kg of pectin; after mixing, add 0.2 kg of cream and 3 kg of whey protein powder to the sterilized milk and mix Dissolve to obtain feed liquid A;
(3)将料液A均质,均质温度55℃,压力120Pa,得到均质后料液A;(3) Homogenize the feed liquid A, the homogenization temperature is 55 ℃, the pressure is 120Pa, and the feed liquid A after the homogenization is obtained;
(4)将均质后料液A巴氏杀菌,温度85℃,时长300秒,得到杀菌后料液A;(4) pasteurize the feed solution A after the homogenization, the temperature is 85 ° C, and the duration is 300 seconds, and the feed solution A after the sterilization is obtained;
(5)将杀菌后料液A冷却至40±3℃,添加菌种并发酵,发酵终点pH4.4,破乳后得到发酵后料液A;(5) cooling the feed liquid A after sterilization to 40±3° C., adding bacterial species and fermenting, the fermentation end point pH 4.4, and obtaining the post-fermentation feed liquid A after demulsification;
(6)将发酵后料液A加热至60-65℃后加入白砂糖15千克,琼脂1.1千克,明胶0.7千克和魔芋胶0.8千克,并搅拌溶解,得到料液B;(6) adding 15 kilograms of white granulated sugar, 1.1 kilograms of agar, 0.7 kilograms of gelatin and 0.8 kilograms of konjac gum after heating the feed liquid A to 60-65°C after fermentation, and stirring and dissolving to obtain feed liquid B;
(7)将料液B杀菌,杀菌温度72℃,时间30秒;(7) sterilize the feed liquid B, the sterilization temperature is 72°C, and the time is 30 seconds;
(8)灌装,灌装时料液的温度为45℃,灌装后于2-6℃下静置至料液凝固(凝固需6小时)。(8) Filling, the temperature of the material liquid during filling is 45°C, and after filling, let it stand at 2-6°C until the material liquid solidifies (it takes 6 hours to solidify).
产品质构特性检测Product texture characteristic testing
方法:method:
检测设备Testing Equipment Brookfield CT3质构仪Brookfield CT3 Texture Analyzer 样品温度sample temperature 25℃25℃
测试类型test type TPA质构分析TPA Texture Analysis 可恢复时间recovery time 0s0s
目标Target 30.0mm30.0mm 同一触发点same trigger point real
等待时间waiting time 0s0s 预测试速度Pre-test speed 2mm/s2mm/s
触发点负载Trigger point load 3.0g3.0g 数据频率data frequency 10points/sec10points/sec
测试速度Test speed 1.00mm/s1.00mm/s 探头probe TA11/1000TA11/1000
返回速度return speed 1.00mm/s1.00mm/s 夹具Fixture TA-RT-KITTA-RT-KIT
循环次数Cycles 2.02.0 负载单元load cell 4500g4500g
对上述实施例制得的凝固型风味酸奶的硬度立即进行检测,结果如下表所示:The hardness of the solidified flavored yogurt prepared in the above-mentioned embodiment is detected immediately, and the results are shown in the following table:
   硬度/gHardness/g
实施例1Example 1 160160
实施例2Example 2 155155
对比例1Comparative Example 1 115115
25℃条件下存放5个月后,对上述实施例制得的凝固型风味酸奶的硬度进行检测,结果如下表所示:After being stored at 25°C for 5 months, the hardness of the solidified flavored yogurt prepared in the above embodiment was tested, and the results are shown in the following table:
   硬度/gHardness/g
实施例1Example 1 161161
实施例2Example 2 151151
对比例1Comparative Example 1 112112
产品硬度反映的是牙齿挤压样品的力量,根据感官测试,酸奶产品硬度达到90g以上才具有凝固型状态,硬度过高口感不好,硬度过低产品在储运及销售过程中质构状态易发生改变。凝固型酸奶适宜的硬度为100-200g。Product hardness reflects the force of the teeth to squeeze the sample. According to the sensory test, the yogurt product has a solidified state when the hardness reaches more than 90g. If the hardness is too high, the taste is not good. If the hardness is too low, the texture state of the product is easy to be stored and sold. changes happened. The suitable hardness of solidified yogurt is 100-200g.
根据上述检测结果,实施例1、实施例2、对比例1均满足凝固型酸奶的质构状态。其中对比例1偏软,实施例1和实施例2软硬较合适。According to the above test results, Example 1, Example 2, and Comparative Example 1 all satisfy the texture state of solidified yogurt. Among them, Comparative Example 1 is soft, and Example 1 and Example 2 are more suitable for softness and hardness.
凝固型风味酸奶的口感风味评价Evaluation of texture and flavor of solidified flavored yogurt
取实施例1-2、对比例1的产品作为口味测试样品,进行口感风味评价实验:品尝人数共200人(18-28岁的男性和女性各100人),分别对实施例1-2的产品以及对比例1的产品进行品尝,采用不记名打分制,对产品进行整体喜好度评分。Take the products of Example 1-2 and Comparative Example 1 as taste test samples, and carry out a taste and flavor evaluation experiment: a total of 200 people (100 men and women aged 18-28), respectively The product and the product of Comparative Example 1 were tasted, and an anonymous scoring system was used to score the overall preference of the product.
评分标准如下:The scoring criteria are as follows:
细则Rules 分值Score
非常喜欢like it very much 55
比较喜欢Quite like it 44
一般,谈不上喜欢不喜欢In general, I can't say whether I like it or not. 33
不太喜欢don't like 22
一点也不喜欢don't like it at all 11
计算方法:Calculation method:
结果=[(非常喜欢)人数+(比较喜欢)人数]/总人数。Result=[(very like) number of people + (more like) number of people]/total number of people.
评估结果如下:The evaluation results are as follows:
项目project 实施例1Example 1 实施例2Example 2 对比例1Comparative Example 1
分数Fraction 9292 8989 8080
从评估结果可以看出,本发明实施例1-2所提供的常温酸奶的口感优于对比例1提供的凝固型风味酸奶。It can be seen from the evaluation results that the taste of the room temperature yogurt provided by Example 1-2 of the present invention is better than that of the solidified flavored yogurt provided by Comparative Example 1.
产品储运过程中质构状态发生改变情况测试Test for changes in texture state during product storage and transportation
方法:装箱5000杯产品,使用常温物流车进行运输,从宁夏吴忠运输到伊利集团液态奶事业部南京分仓(运输距离约1600公里,汽车运输时间大于20小时),产品储运过程中避免跌落和剧烈摇动,避免产品横放、倾斜或倒置,严禁暴力装运。在分仓随机抽取1000杯产品进行状态检测,抽检结果如下表。Method: Pack 5,000 cups of products, use normal temperature logistics vehicles for transportation, and transport them from Wuzhong, Ningxia to Nanjing branch of Yili Group's liquid milk business department (transportation distance is about 1,600 kilometers, and vehicle transportation time is more than 20 hours). Avoid product storage and transportation. Drop and shake violently, avoid placing the product horizontally, tilted or upside down, and violent shipment is strictly prohibited. 1000 cups of products are randomly selected from the sub-warehouses for state testing. The sampling results are shown in the table below.
   产品质构状态破碎率/%Product texture state breakage rate/%
实施例1Example 1 0.20.2
实施例2Example 2 0.30.3
对比例1Comparative Example 1 23twenty three
产品质构状态破碎的样品为产品的凝固型状态因物理外力作用发生改变的样品。The sample whose texture state is broken is the sample whose solidification state of the product is changed due to the action of physical external force.
根据上述检测结果,实施例1和实施例2的破损率明显低于对比例1,说明实施例1和实施例2的硬度更适宜常温进行运输,对比例1的产品硬度偏软,常温运输时产品质构状态易发生改变。According to the above test results, the breakage rate of Example 1 and Example 2 is significantly lower than that of Comparative Example 1, indicating that the hardness of Example 1 and Example 2 is more suitable for transportation at room temperature, while the hardness of the product of Comparative Example 1 is softer, and when transported at room temperature The texture state of the product is prone to change.
产品货架期稳定性检测Product shelf life stability testing
方法:将产品分别在常温(25℃)、37℃、45℃条件下进行存放,观察产品乳清析 出情况,观察结果如下:Method: Store the product at normal temperature (25°C), 37°C, and 45°C, observe the whey precipitation of the product, and observe the results as follows:
   37℃放置1个月1 month at 37°C 37℃放置2个月2 months at 37°C
实施例1Example 1 无明显乳清析水No obvious whey separation 无明显乳清析水No obvious whey separation
实施例2Example 2 无明显乳清析水No obvious whey separation 无明显乳清析水No obvious whey separation
对比例1Comparative Example 1 少量乳清析水A small amount of whey dehydrated 较多乳清析出more whey
   45℃放置1个月45℃ for 1 month 45℃放置2个月2 months at 45°C
实施例1Example 1 无明显乳清析水No obvious whey separation 少量乳清析水A small amount of whey dehydrated
实施例2Example 2 无明显乳清析水No obvious whey separation 少量乳清析水A small amount of whey dehydrated
对比例1Comparative Example 1 较多乳清析水more whey water 较多乳清析出more whey
Figure PCTCN2021107508-appb-000001
Figure PCTCN2021107508-appb-000001
产品微生物检测Product Microbiological Testing
方法:按GB4789.1《食品微生物学检验总则》和GB4789.18《食品微生物学检验乳与乳制品检验》执行Method: According to GB4789.1 "Food Microbiological Inspection General Rules" and GB4789.18 "Food Microbiological Inspection Milk and Dairy Product Inspection"
对上述实施例制得的凝固型风味酸奶的微生物立即进行检测,检测结果如下:The microorganisms of the solidified flavored yogurt prepared in the above-described embodiment are detected immediately, and the detection results are as follows:
Figure PCTCN2021107508-appb-000002
Figure PCTCN2021107508-appb-000002
将上述实施例制得的凝固型风味酸奶在常温(25℃)条件下储存6个月,进行微生物检测,结果如下表所示:The solidified flavored yogurt prepared in the above embodiment was stored for 6 months under normal temperature (25° C.) conditions, and the microorganisms were detected. The results are as shown in the following table:
Figure PCTCN2021107508-appb-000003
Figure PCTCN2021107508-appb-000003
Figure PCTCN2021107508-appb-000004
Figure PCTCN2021107508-appb-000004
以上所述的具体实施例,对本发明的目的、技术方案和有益效果进行了进一步详细说明,应理解的是,以上所述仅为本发明的具体实施例而已,并不用于限制本发明,凡在本发明的精神和原则之内,所做的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The specific embodiments described above further describe the purpose, technical solutions and beneficial effects of the present invention in further detail. It should be understood that the above are only specific embodiments of the present invention, and are not intended to limit the present invention. Within the spirit and principle of the present invention, any modifications, equivalent replacements, improvements, etc. made should be included within the protection scope of the present invention.

Claims (20)

  1. 一种凝固型风味酸奶的制备方法,其特征在于,包括:A preparation method of solidified flavored yogurt, characterized in that, comprising:
    (1)将原料乳杀菌后经过RO膜过滤设备分离得到RO截留液和RO渗透液;(1) after the raw milk is sterilized, the RO retentate and the RO permeate are obtained through RO membrane filtration equipment separation;
    (2)将部分所述RO截留液与糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯混合均匀,得到基料;(2) mixing part of the RO retentate with sugar, whey protein powder, cheese powder, mono- and diglycerol fatty acid esters to obtain base material;
    (3)将所述基料均质、杀菌后接入发酵菌种进行发酵,发酵后破乳得到发酵乳;(3) the base material is homogenized and sterilized and then connected to a fermented strain for fermentation, and the demulsification after fermentation is performed to obtain fermented milk;
    (4)将所述发酵乳与利用所述RO渗透液、果胶和糖制备的第一胶液混合,杀菌,冷却;(4) mixing the fermented milk with the first glue prepared by utilizing the RO permeate, pectin and sugar, sterilizing, and cooling;
    (5)利用所述RO渗透液与琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶、糖中的两种或多种原料混合制备第二胶液,将步骤(4)所得的料液加热后与所述第二胶液混合;(5) utilize described RO permeate to mix with two or more raw materials in agar, gelatin, carrageenan, xanthan gum, locust bean gum, sugar to prepare the second glue solution, the feed solution obtained in step (4) After heating, it is mixed with the second glue solution;
    (6)无菌热灌装后进行在线冷却,得到凝固型风味酸奶。(6) on-line cooling after aseptic hot filling to obtain solidified flavored yogurt.
  2. 根据权利要求1所述的制备方法,其中,步骤(1)中,分离出的所述RO渗透液为所述原料乳质量的10-40%。The preparation method according to claim 1, wherein, in step (1), the separated RO permeate is 10-40% of the mass of the raw milk.
  3. 根据权利要求2所述的制备方法,其中,步骤(1)中,原料乳杀菌的温度为70-89℃。The preparation method according to claim 2, wherein, in step (1), the temperature of raw milk sterilization is 70-89°C.
  4. 根据权利要求3所述的制备方法,其中,步骤(1)中,原料乳杀菌的温度为70-75℃。The preparation method according to claim 3, wherein, in step (1), the temperature for sterilizing the raw milk is 70-75°C.
  5. 根据权利要求2所述的制备方法,其中,步骤(1)中,原料乳杀菌的杀菌时间为10-20s。The preparation method according to claim 2, wherein, in step (1), the sterilization time of the raw milk sterilization is 10-20s.
  6. 根据权利要求1所述的制备方法,其中,步骤(2)包括将占发酵乳配料量50%-70%的膜过滤RO截留液加热至60-65℃,加入糖、乳清蛋白粉、芝士粉、单,双甘油脂肪酸酯,混合均匀,然后加入剩余的RO截留液,并混合均匀。The preparation method according to claim 1, wherein step (2) comprises heating the membrane filtration RO retentate which accounts for 50%-70% of the fermented milk ingredients to 60-65°C, adding sugar, whey protein powder, cheese Powder, mono- and diglycerol fatty acid esters, mix well, then add the remaining RO retentate, and mix well.
  7. 根据权利要求1所述的制备方法,其中,步骤(3)中,均质的温度为60-65℃,均质压力为30/160bar。The preparation method according to claim 1, wherein, in step (3), the homogenizing temperature is 60-65° C., and the homogenizing pressure is 30/160 bar.
  8. 根据权利要求1所述的制备方法,其中,步骤(3)中,杀菌温度为90-100℃,杀菌时间为2-10min。The preparation method according to claim 1, wherein, in step (3), the sterilization temperature is 90-100°C, and the sterilization time is 2-10min.
  9. 根据权利要求1所述的制备方法,其中,步骤(3)中,发酵温度为42-44℃,发酵终点为110-125°T,pH为4.2-4.4。The preparation method according to claim 1, wherein, in step (3), the fermentation temperature is 42-44° C., the fermentation end point is 110-125° C., and the pH is 4.2-4.4.
  10. 根据权利要求1所述的制备方法,其中,步骤(4)中,所述第一胶液的制备方法为:将占第一胶液配料量91-92%的RO渗透液加热至60-80℃后,加入果胶和占第一 胶液配料量3-5%的糖,并混合均匀,第一胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到40-45℃。The preparation method according to claim 1, wherein, in step (4), the preparation method of the first glue solution is as follows: the RO permeate, which accounts for 91-92% of the first glue solution, is heated to 60-80% After ℃, add pectin and sugar that accounts for 3-5% of the first glue solution, and mix them evenly. The first glue solution is sterilized at 110°C-130°C/40-45s, and cooled to 40-45°C .
  11. 根据权利要求1所述的制备方法,其中,步骤(4)中,杀菌温度为70-80℃,杀菌时间为20-30s,冷却温度为50-70℃。The preparation method according to claim 1, wherein, in step (4), the sterilization temperature is 70-80°C, the sterilization time is 20-30s, and the cooling temperature is 50-70°C.
  12. 根据权利要求1所述的制备方法,其中,步骤(4)中,所述发酵乳与所述第一胶液混合的混合质量比为8:1,混合温度为40-50℃。The preparation method according to claim 1, wherein, in step (4), the mixing mass ratio of the fermented milk and the first glue solution is 8:1, and the mixing temperature is 40-50°C.
  13. 根据权利要求1所述的制备方法,其中,步骤(5)中,所述第二胶液的制备方法为:将RO渗透液加热至60-80℃后,加入琼脂、明胶、卡拉胶、黄原胶、刺槐豆胶中的两种或多种原料及占第二胶液配料量4-6%的糖,并混合均匀,第二胶液进行110℃-130℃/40-45s的杀菌处理,并冷却到50-65℃。The preparation method according to claim 1, wherein, in step (5), the preparation method of the second glue solution is: after the RO permeate is heated to 60-80 DEG C, add agar, gelatin, carrageenan, yellow Two or more raw materials in the original gum and locust bean gum and sugar accounting for 4-6% of the second glue solution are mixed evenly, and the second glue solution is sterilized at 110°C-130°C/40-45s , and cooled to 50-65°C.
  14. 根据权利要求1所述的制备方法,其中,步骤(5)中,步骤(4)所得的料液加热温度为45-60℃。The preparation method according to claim 1, wherein, in step (5), the heating temperature of the feed liquid obtained in step (4) is 45-60°C.
  15. 根据权利要求1所述的制备方法,其中,步骤(5)中,步骤(4)所得的料液加热后与所述第二胶液混合的混合质量比为9:1,混合温度为45-60℃。The preparation method according to claim 1, wherein, in step (5), the mixing mass ratio of the material liquid obtained in step (4) after heating with the second glue liquid is 9:1, and the mixing temperature is 45- 60°C.
  16. 根据权利要求1所述的制备方法,其中,步骤(6)中,灌装温度为45-60℃,冷却温度为30℃以下。The preparation method according to claim 1, wherein, in step (6), the filling temperature is 45-60°C, and the cooling temperature is below 30°C.
  17. 根据权利要求1所述的制备方法,其中,所述凝固型风味酸奶的原料的重量百分比为:原料乳86.2%-91%、糖6%-10%、乳清蛋白粉0.1%-0.5%、芝士粉0.2%-0.3%、单,双甘油脂肪酸酯0.05%-0.1%、果胶0.3%-0.6%、琼脂0%-0.5%、明胶0-0.6%、卡拉胶0%-1%、黄原胶0%-0.1%和刺槐豆胶0%-0.2%。The preparation method according to claim 1, wherein the weight percentage of the raw materials of the solidified flavored yogurt is: 86.2%-91% of raw milk, 6%-10% of sugar, 0.1%-0.5% of whey protein powder, Cheese powder 0.2%-0.3%, mono, diglyceride fatty acid ester 0.05%-0.1%, pectin 0.3%-0.6%, agar 0%-0.5%, gelatin 0-0.6%, carrageenan 0%-1%, Xanthan gum 0%-0.1% and locust bean gum 0%-0.2%.
  18. 根据权利要求17所述的制备方法,其中,所述原料乳为生牛乳或生羊乳。The preparation method according to claim 17, wherein the raw milk is raw cow milk or raw goat milk.
  19. 根据权利要求17所述的制备方法,其中,所述糖为白砂糖、果葡糖浆、蔗糖、葡萄糖、葡萄糖浆、果糖、麦芽糖、麦芽糖浆、乳糖中的一种或多种。The preparation method according to claim 17, wherein the sugar is one or more of white granulated sugar, fructose syrup, sucrose, glucose, glucose syrup, fructose, maltose, maltose syrup, and lactose.
  20. 一种利用权利要求1-19任一项所述制备方法制备的凝固型风味酸奶。A solidified flavored yogurt prepared by the preparation method of any one of claims 1-19.
PCT/CN2021/107508 2020-10-29 2021-07-21 Set-style flavor yogurt and preparation method therefor WO2022088777A1 (en)

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