CN111256663A - Centering calibration method and device - Google Patents
Centering calibration method and device Download PDFInfo
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- CN111256663A CN111256663A CN201811465730.3A CN201811465730A CN111256663A CN 111256663 A CN111256663 A CN 111256663A CN 201811465730 A CN201811465730 A CN 201811465730A CN 111256663 A CN111256663 A CN 111256663A
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Abstract
The method is applied to wide-angle light scattering equipment and comprises the steps of sequentially adjusting a rotating arm to multiple rotation angles α, controlling an image acquisition component to acquire images of a reference object under each rotation angle α, determining a standard center imaging position according to the images of the reference object acquired under multiple rotation angles α, wherein the standard center imaging position is the imaging position of a rotation center in the images acquired by the image acquisition component, and adjusting the position of a sample bin according to the standard center imaging position.
Description
Technical Field
The disclosure relates to the technical field of optical scattering, and in particular to a centering calibration method and device.
Background
Wide angle light scattering is a technique for determining relevant parameters in a sample by measuring the light intensity or changes in light intensity of scattered light at multiple scattering angles. For example, by wide angle static light scattering, the average molecular weight of the particles in the sample can be determined; by wide angle dynamic light scattering, the particle diameter of the particles in the sample, etc. can be determined.
Fig. 1 is a schematic view showing a structure of a wide-angle light scattering device in the related art. As shown in fig. 1, laser emitted from the laser passes through the slit and the converging lens and then irradiates onto the center of the sample chamber, the detection light path and the detector rotate to receive scattered light corresponding to different scattering angles, and the electronic circuit determines the light intensity or the light intensity variation of the received scattered light, thereby determining the related parameters of the sample in the sample chamber.
In wide-angle light scattering measurements, the scattering angle is an important parameter affecting the measurement result, e.g. the light intensity error is 6% for every 1 degree deviation of the scattering angle. In order to ensure the accuracy of the scattering angle, it is necessary to ensure that the rotation center of the detection optical path coincides with the center of the sample chamber (hereinafter referred to as centering).
The existing centering calibration process includes: firstly, adjusting the position of a sample cabin to be basically positioned near the rotation center of a detection light path; secondly, placing a reference object in the sample chamber, wherein the center of the reference object is easy to see through human eyes (for example, a needle point processed by special processing is arranged), and the center of the reference object is superposed with the center of the sample chamber; and finally, further adjusting the position of the sample bin, so that when the center of the reference object is observed at a plurality of angles through the ocular lens, the center of the reference object is coincided with the rotation center of the detection light path.
Therefore, the existing centering calibration method is manual calibration, and the calibration process needs to repeatedly execute the adjustment step, so that the calibration process is time-consuming and long.
Disclosure of Invention
In view of this, the present disclosure provides a centering calibration method and device, which can simplify the operation steps of the centering calibration process and effectively shorten the time consumption of the centering calibration process.
According to a first aspect of the disclosure, a centering calibration method is provided, and the method is applied to a wide-angle light scattering device, the wide-angle light scattering device comprises a sample bin, a rotating arm and an image collecting component, the sample bin is located at one end close to a rotating center of the rotating arm, the image collecting component is mounted at one end, far away from the rotating center, of the rotating arm, a reference object is arranged in the sample bin, and the center of the reference object is overlapped with the center of the sample bin, the method comprises the steps of sequentially adjusting the rotating arm to multiple rotating angles α, controlling the image collecting component to collect images of the reference object at each rotating angle α, determining a standard center imaging position according to the images of the reference object collected at the multiple rotating angles α, and adjusting the position of the sample bin according to the standard center imaging position.
In one possible implementation, the determining of the standard center imaging position according to the images of the reference object acquired at the plurality of rotation angles α comprises determining the reference object center imaging position at the rotation angle α according to the image of the reference object acquired at any rotation angle α, wherein the reference object center imaging position is the imaging position of the center of the reference object in the image acquired by the image acquisition component, and obtaining the standard center imaging position according to the reference object center imaging position at each rotation angle α.
In one possible implementation, adjusting the position of the sample compartment according to the standard center imaging position includes sequentially adjusting the rotating arm to a plurality of rotation angles β, and adjusting the position of the sample compartment in a direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at each rotation angle β until the reference center imaging position coincides with the standard center imaging position.
In one possible implementation, adjusting the position of the sample bin based on the isocenter imaging position includes: adjusting the rotating arm to a rotation angle theta; adjusting the position of the sample bin in a direction which is parallel to the rotation plane of the rotating arm and is vertical to the rotating arm at a rotation angle theta until the central imaging position of the reference object coincides with the standard central imaging position; adjusting the rotating arm to a rotating angle phi perpendicular to the rotating angle theta; and adjusting the position of the sample bin in a direction which is parallel to the rotating plane of the rotating arm and is vertical to the rotating arm under the rotating angle phi until the central imaging position of the reference object coincides with the standard central imaging position.
In a possible implementation manner, the wide-angle light scattering device further includes a laser, a sample to be measured is placed in the center of the sample chamber, and laser emitted by the laser irradiates the sample chamber; the method further comprises the following steps: adjusting the rotating arm within a preset rotating angle range, and controlling the image acquisition component to acquire a plurality of images of the tested sample within the preset angle range, wherein the preset rotating angle range comprises an initial 0 degree; determining a plurality of light signal intensity values corresponding to the standard center imaging position according to a plurality of images of the detected sample collected within the preset rotation angle range; determining a rotation angle corresponding to a maximum light signal intensity value in the plurality of light signal intensity values; and determining a standard zero rotation angle corresponding to the rotary arm according to the rotation angle corresponding to the maximum optical signal intensity value.
In a possible implementation manner, determining a standard zero rotation angle corresponding to the rotary arm according to the rotation angle corresponding to the maximum optical signal strength value includes one of: if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is smaller than a preset threshold value, determining the initial 0 degree as the standard zero rotation angle; and if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is greater than or equal to the preset threshold value, determining the rotation angle corresponding to the maximum optical signal intensity value as the standard zero rotation angle.
According to a second aspect of the disclosure, a centering calibration device is provided, which is applied to a wide-angle light scattering device, the wide-angle light scattering device includes a sample chamber, a rotating arm and an image collecting component, the sample chamber is located at one end close to a rotating center of the rotating arm, the image collecting component is mounted at one end, far away from the rotating center, of the rotating arm, a reference object is arranged in the sample chamber, and a center of the reference object coincides with a center of the sample chamber, the device includes an angle adjusting module for sequentially adjusting the rotating arm to a plurality of rotating angles α, an acquisition module for controlling the image collecting component to collect an image of the reference object at each rotating angle α, a first determining module for determining a standard center imaging position according to the images of the reference object collected at the plurality of rotating angles α, wherein the standard center imaging position is an imaging position of the rotating center in the image collected by the image collecting component, and a position adjusting module for adjusting a position of the sample chamber according to the standard center imaging position.
In a possible implementation manner, the first determining module comprises a first determining submodule and a second determining submodule, wherein the first determining submodule is used for determining a reference object center imaging position under any rotation angle α according to the image of the reference object acquired under any rotation angle α, the reference object center imaging position is the imaging position of the center of the reference object in the image acquired by the image acquisition component, and the second determining submodule is used for obtaining the standard center imaging position according to the reference object center imaging position under each rotation angle α.
In a possible implementation manner, the angle adjusting module is further configured to sequentially adjust the rotating arm to a plurality of rotation angles β, and the position adjusting module is further configured to adjust the position of the sample chamber in a direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at each rotation angle β until the reference object central imaging position coincides with the standard central imaging position.
In a possible implementation manner, the wide-angle light scattering device further includes a laser, a sample to be measured is placed in the center of the sample chamber, and laser emitted by the laser irradiates the sample chamber; the angle adjusting module is also used for adjusting the rotating arm within a preset rotating angle range; the acquisition module is further configured to control the image acquisition component to acquire a plurality of images of the sample to be detected within the preset angle range, where the preset rotation angle range includes an initial 0 degree; the device further comprises: the second determining module is used for determining a plurality of optical signal intensity values corresponding to the standard center imaging position according to a plurality of images of the detected sample acquired within the preset rotation angle range; a third determining module, configured to determine a rotation angle corresponding to a maximum light signal intensity value of the plurality of light signal intensity values; and the fourth determining module is used for determining a standard zero rotation angle corresponding to the rotating arm according to the rotation angle corresponding to the maximum optical signal strength value.
The rotating arm is adjusted to a plurality of rotating angles α in sequence, the image acquisition component is controlled to acquire the image of the reference object under each rotating angle α, and the standard center imaging position is determined according to the image of the reference object acquired under a plurality of rotating angles α, wherein the standard center imaging position is the imaging position of the rotating center in the image acquired by the image acquisition component, so that the position of the sample bin can be adjusted according to the standard center imaging position, blind adjustment of the sample bin can be avoided, the operation steps of the centering calibration process are simplified, and the time consumption of the centering calibration process is effectively reduced.
Other features and aspects of the present disclosure will become apparent from the following detailed description of exemplary embodiments, which proceeds with reference to the accompanying drawings.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate exemplary embodiments, features, and aspects of the disclosure and, together with the description, serve to explain the principles of the disclosure.
Fig. 1 is a schematic view showing a configuration of a wide-angle light scattering device in the related art;
FIG. 2 shows a schematic flow chart of a centering calibration method according to an embodiment of the present disclosure;
FIG. 3 shows a schematic diagram of a wide-angle light scattering device of an embodiment of the present disclosure;
FIG. 4 shows a schematic structural view of a reference of an embodiment of the present disclosure;
fig. 5 shows a schematic flow chart of step S22 of the centering calibration method according to an embodiment of the disclosure;
fig. 6 shows a schematic flow chart of step S23 of the centering calibration method according to an embodiment of the disclosure;
fig. 7 shows a schematic flow chart of step S23 of the centering calibration method according to an embodiment of the disclosure;
FIG. 8 shows a schematic view of adjusting the position of a sample compartment according to an embodiment of the present disclosure;
FIG. 9 shows a schematic flow chart of a centering calibration method according to an embodiment of the present disclosure;
fig. 10 shows a block diagram of a centering calibration apparatus of an embodiment of the present disclosure.
Detailed Description
Various exemplary embodiments, features and aspects of the present disclosure will be described in detail below with reference to the accompanying drawings. In the drawings, like reference numbers can indicate functionally identical or similar elements. While the various aspects of the embodiments are presented in drawings, the drawings are not necessarily drawn to scale unless specifically indicated.
The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present disclosure. It will be understood by those skilled in the art that the present disclosure may be practiced without some of these specific details. In some instances, methods, means, elements and circuits that are well known to those skilled in the art have not been described in detail so as not to obscure the present disclosure.
Fig. 2 shows a schematic flow chart of a centering calibration method according to an embodiment of the present disclosure. The method can be applied to wide-angle light scattering devices.
Fig. 3 shows a schematic diagram of a wide-angle light scattering device of an embodiment of the present disclosure. As shown in fig. 3, the wide-angle light scattering device includes a sample chamber, a rotating arm and an image collecting component, the sample chamber is located at one end close to the rotation center of the rotating arm, the image collecting component is installed at one end of the rotating arm far away from the rotation center, a reference object is arranged in the sample chamber, and the center of the reference object coincides with the center of the sample chamber.
As shown in fig. 2, the method may include:
step S21, sequentially adjusting the rotating arm to a plurality of rotation angles α, and controlling the image capturing part to capture an image of the reference object at each rotation angle α.
Step S22, determining a standard center imaging position from the images of the reference object acquired at the plurality of rotation angles α, wherein the standard center imaging position is an imaging position of the rotation center in the image acquired by the image acquisition component;
and step S23, adjusting the position of the sample bin according to the standard center imaging position.
Fig. 4 shows a schematic structural diagram of a reference object of an embodiment of the present disclosure. In one example, as shown in fig. 4, when the sample chamber is a cylindrical structure, the reference object may be a cylindrical precision metal piece. The outer diameter of the reference object is equal to the inner diameter of the sample chamber, and the reference object can be placed in the sample chamber in a fitting mode, so that the center of the reference object is coincident with the center of the sample chamber. The center of the reference object is provided with an easily recognizable tip (or other easily recognizable marker) so that the imaging position of the center of the reference object can be easily recognized in the image of the reference object captured by the image capturing section.
The mid-calibration process will be described in detail by taking the wide-angle light scattering device shown in fig. 3 as an example.
Sequentially adjusting the rotating arm to a plurality of rotation angles α (α)i1,2, 3.) and α at each rotation angleiAnd the lower control image acquisition component acquires an image of the reference object.
For example, four rotation angles α: α are used1、α2、α3And α4When the rotation angle of the rotating arm is adjusted to α1At the time of controlling the image pickup section to pick up the reference object at the rotation angle α1When the rotation angle of the rotary arm is adjusted to α2At the time of controlling the image pickup section to pick up the reference object at the rotation angle α2When the rotation angle of the rotary arm is adjusted to α3At the time of controlling the image pickup section to pick up the reference object at the rotation angle α3When the rotation angle of the rotary arm is adjusted to α4Time, controlThe image acquisition component acquires the reference object at a rotation angle α4The following images.
It should be noted that the number of the rotation angles α is greater than or equal to three, and the specific number may be determined according to actual situations, which is not specifically limited by the present disclosure.
When the rotating arm is adjusted to a plurality of rotating angles α, it is not necessary to ensure that the initial 0 degree corresponding to the rotating arm is the standard zero rotating angle, that is, even if the rotating angle α is the corresponding angle when the standard zero rotating angle has a deviation, the centering calibration process will not be affected.
From the images of the reference acquired by the image acquisition component at the plurality of rotation angles α, a standard center imaging position may be determined, wherein the standard center imaging position is an imaging position of the center of rotation of the rotary arm in the image acquired by the image acquisition component.
Fig. 5 shows a flowchart of step S22 of the centering calibration method according to an embodiment of the disclosure. As shown in fig. 5, step S22 may include:
step S221, determining a reference object center imaging position at any rotation angle α according to the image of the reference object acquired at any rotation angle α, wherein the reference object center imaging position is an imaging position of the center of the reference object in the image acquired by the image acquisition component;
in step S222, a standard central imaging position is obtained from the central imaging position of the reference object at each rotation angle α.
From the images of the reference object acquired at the plurality of rotation angles α, the imaging position of the center of the reference object in each image, that is, the imaging position of the center of the reference object at each rotation angle α, is determined, respectively.
In one example, the coordinate values of the imaging position of the center of the reference object at each rotation angle α in the image may be (z:)1,a1)、(z2,a2)、(z3,a3) … … are provided. Wherein the z direction is parallel to the rotation plane of the rotary arm, and the a direction is perpendicular to the rotation plane of the rotary arm.
From the abscissa value of the reference object center imaging position in the image at each rotation angle α, the abscissa value of the standard center imaging position in the image can be determined using the following formula:
wherein, the expression of the matrix A is:
inv denotes the matrix inversion operation, ATA transposed matrix, z, representing the matrix A0The determined standard center imaging position is an abscissa value in the image.
In one example, when four rotation angles α: α are used10 degree, α2α degree ═ 45 °390 degrees and α4When the standard central imaging position is 135 degrees, the abscissa value of the standard central imaging position in the image determined according to the above formula is:
z0＝0.854·z1+0.354·z2-0.354·z3+0.854·z4。
because the z direction is parallel to the rotation plane of the rotating arm, after the abscissa value of the standard center imaging position is determined, the position of the sample bin can be adjusted according to the determined abscissa value of the standard center imaging position, and finally, the center of the sample bin and the rotation center of the rotating arm can be superposed in the plane parallel to the rotation plane of the rotating arm, so that centering calibration is completed.
Since the a direction is perpendicular to the rotation plane of the rotary arm, regardless of the centering calibration process, it is not necessary to determine the ordinate value of the standard center imaging position.
Fig. 6 shows a flowchart of step S23 of the centering calibration method according to an embodiment of the disclosure. As shown in fig. 6, step S23 may include:
step S231, sequentially adjusts the rotating arm to a plurality of rotation angles β.
Step S232, the position of the sample chamber is adjusted in the direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at each rotation angle β until the central imaging position of the reference coincides with the standard central imaging position.
The above-mentioned determination of the abscissa value z of the standard center imaging position in the image0Then, the z direction is parallel to the rotation plane of the rotating arm, so that the rotating arm can be sequentially adjusted to a plurality of rotation angles β in the rotation plane of the rotating arm, the position of the sample bin is adjusted in the direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm for each rotation angle β, the image collecting component is controlled to collect the image of the reference object after the position of the sample bin is adjusted each time until the imaging position of the standard center in the collected image is coincident with the imaging position of the center of the reference object, at the moment, the center of the sample bin and the rotation center of the rotating arm are coincident in the direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm, and the center of the sample bin and the rotation center of the rotating arm can be coincident in the directions parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm by adjusting the positions of the sample bins under a plurality of rotation angles β, and finally the center of the sample bin and the rotation center of.
Fig. 7 shows a flowchart of step S23 of the centering calibration method according to an embodiment of the disclosure. As shown in fig. 7, step S23 may include:
in step S233, the rotation arm is adjusted to the rotation angle θ.
And S234, adjusting the position of the sample bin in the direction which is parallel to the rotation plane of the rotating arm and is vertical to the rotating arm at the rotation angle theta until the central imaging position of the reference object coincides with the standard central imaging position.
Step S235, adjust the rotating arm to a rotation angle phi perpendicular to the rotation angle theta.
And step S236, adjusting the position of the sample bin in the direction which is parallel to the rotation plane of the rotating arm and is vertical to the rotating arm at the rotation angle phi until the central imaging position of the reference object coincides with the standard central imaging position.
In order to simplify the adjustment process of the position of the sample bin, the rotating arm can be sequentially adjusted to two mutually perpendicular rotating angles, the position of the sample bin is adjusted under each rotating angle, and finally the coincidence of the center of the sample bin and the rotating center of the rotating arm is realized.
Fig. 8 shows a schematic diagram of adjusting the position of a sample compartment according to an embodiment of the present disclosure.
As shown in fig. 8, a is the center of the sample chamber and B is the center of rotation of the rotating arm. The X direction is perpendicular to the rotating arm with the rotating angle of 90 degrees, the Y direction is perpendicular to the rotating arm with the rotating angle of 0 degrees, and the plane of the X direction and the plane of the Y direction are parallel to the rotating plane of the rotating arm.
Adjusting the rotation angle of the rotating arm to 0 degree (X direction), further adjusting the position of the sample bin in the Y direction, and after adjusting the position of the sample bin each time, controlling the image acquisition component to acquire the image of the reference object until the standard center imaging position coincides with the reference object center imaging position in the acquired image, at the moment, the center of the sample bin is A ', and the center A' of the sample bin coincides with the rotation center B of the rotating arm in the Y direction;
the rotation angle of the rotating arm is adjusted to 90 degrees (Y direction) again, the position of the sample bin is adjusted in the X direction, and after the position of the sample bin is adjusted each time, the image acquisition component is controlled to acquire the image of the reference object until the standard center imaging position in the acquired image is superposed with the center imaging position of the reference object, at the moment, the center of the sample bin is A ', and the center A' of the sample bin is superposed with the rotation center B of the rotating arm in the X direction; at this point, the center of the sample compartment coincides with the center of the rotating arm.
The standard center imaging position is determined, so that blind adjustment of the sample bin can be avoided, the operation steps of the centering calibration process are simplified, and the time consumption of the centering calibration process is effectively reduced. In addition, because the method does not depend on manual centering calibration, the centering calibration result is objective and reliable and is not influenced by the experience and the capability of an operator.
Since the operation steps of the centering calibration process are simplified and the time consumption of the centering calibration process is reduced, the centering calibration of the wide-angle light-scattering device can be performed before each measurement using the wide-angle light-scattering device, so that the measurement error can be reduced.
In a possible implementation mode, the wide-angle light scattering device further comprises a laser, the sample to be measured is placed in the center of the sample cabin, and laser emitted by the laser irradiates the sample cabin. Before the measurement of the measured sample is performed by using the wide-angle light scattering device, the standard zero rotation angle of the rotary arm needs to be determined (or calibrated) so that the standard zero rotation angle of the rotary arm coincides with the laser incidence direction passing through the center of the sample chamber.
Fig. 9 shows a flowchart of a centering calibration method according to an embodiment of the disclosure. As shown in fig. 9, the method may further include:
and step S24, adjusting the rotating arm within a preset rotating angle range, and controlling the image acquisition component to acquire a plurality of images of the tested sample within the preset angle range, wherein the preset rotating angle range comprises an initial 0 degree.
Step S25, determining a plurality of light signal intensity values corresponding to the standard center imaging position according to a plurality of images of the measured sample collected within the preset rotation angle range.
In step S26, the rotation angle corresponding to the maximum light signal intensity value among the plurality of light signal intensity values is determined.
And step S27, determining a standard zero rotation angle corresponding to the rotary arm according to the rotation angle corresponding to the maximum optical signal intensity value.
For example, the sample to be measured may be placed in the center of the sample chamber, the laser is turned on, and the laser emitted from the laser is focused by the converging lens and then irradiated to the sample to be measured, as shown in fig. 3.
An initial 0 degree is set for the rotary arm and a plurality of images of the sample under test are acquired within a preset range of rotational angles including the initial 0 degree. For example, the rotation angle of the rotary arm is adjusted stepwise within a range of-3 degrees to +3 degrees, and an image of the sample to be measured is acquired at each rotation angle. The present disclosure does not limit the specific values of the predetermined rotation angle range.
Since the preset angle range is near the initial 0 degree, which is equivalent to direct light collection, in order to avoid overexposure of the image collected by the image collecting component caused by too large light signal intensity, the laser can be adjusted to the minimum output power, or, as shown in fig. 3, an electrically controlled adjustable attenuation sheet can be further arranged and adjusted to the maximum attenuation position.
According to a plurality of images of the detected sample collected within a preset rotation angle range, respectively determining the light signal intensity corresponding to the standard center imaging position in each image, namely determining the scattered light signal intensity corresponding to the detected sample in each image; further, a rotation angle corresponding to the maximum optical signal intensity among the plurality of optical signal intensities determined is determined.
Because the intensity of the optical signal corresponding to the laser passing through the center of the sample bin is the maximum, the standard zero rotation angle corresponding to the rotating arm is determined according to the rotation angle corresponding to the maximum optical signal intensity.
In a possible embodiment, the determining a standard zero rotation angle corresponding to the rotary arm according to the rotation angle corresponding to the maximum optical signal strength value includes one of: if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is smaller than a preset threshold value, determining the initial 0 degree as a standard zero rotation angle; and if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is greater than or equal to a preset threshold value, determining the rotation angle corresponding to the maximum optical signal intensity value as a standard zero rotation angle. The present disclosure is not limited to specific values of the preset threshold.
For example, the preset threshold is set to 0.1 degree, when the rotation angle corresponding to the maximum optical signal intensity is-0.05 degree, the angle difference between the rotation angle corresponding to the maximum optical signal intensity and the initial 0 degree is smaller than the preset threshold of 0.1 degree, at this time, it indicates that the zero rotation angle position of the rotating arm is not shifted, and the initial 0 degree is determined as the standard zero rotation angle after calibration;
when the rotation angle corresponding to the maximum optical signal intensity is 2 degrees, the angle difference between the rotation angle corresponding to the maximum optical signal intensity and the initial 0 degree is greater than a preset threshold value of 0.1 degree, at this time, it indicates that the zero-angle position of the rotating arm is deviated, the zero-rotation angle of the rotating arm needs to be calibrated, and 2 degrees are determined as the standard zero-rotation angle after calibration.
After the standard zero rotation angle of the rotating arm is determined (or calibrated), the rotation angle of the rotating arm is the scattering angle corresponding to the measured sample, and then the rotating arm can be adjusted to multiple rotation angles, so that the intensity of the scattering light signals corresponding to the measured sample under different scattering angles is obtained, and the wide-angle light scattering measurement is completed.
The standard zero rotation angle of the rotating arm in the prior art is typically artificially defined, so that small changes in the mechanical position may introduce additional errors. Before the measured sample is measured, the standard zero rotation angle of the rotating arm is determined (or calibrated) by adopting the method, so that the accuracy of the measurement result can be effectively improved.
Fig. 10 shows a block diagram of a centering calibration apparatus according to an embodiment of the present disclosure. The device can be applied to wide angle light scattering equipment, including sample storehouse, swinging boom and image acquisition part in the wide angle light scattering equipment, the sample storehouse is located the one end of being close to the rotation center of swinging boom, and the image acquisition part is installed and is kept away from the one end of rotation center on the swinging boom, is provided with the reference in the sample storehouse, and the center of reference coincides with the center in sample storehouse. As shown in fig. 10, the apparatus 100 includes:
an angle adjusting module 101, configured to sequentially adjust the rotating arm to a plurality of rotation angles α;
an acquisition module 102 for controlling the image acquisition component to acquire an image of the reference at each rotation angle α;
a first determination module 103, configured to determine a standard center imaging position according to the images of the reference object acquired at the plurality of rotation angles α, where the standard center imaging position is an imaging position of a rotation center in the image acquired by the image acquisition component;
and the position adjusting module 104 is used for adjusting the position of the sample bin according to the standard central imaging position.
In one possible implementation, the first determining module 103 includes:
a first determination submodule for determining a reference center imaging position at any rotation angle α from an image of the reference acquired at α, wherein the reference center imaging position is an imaging position of the center of the reference in the image acquired by the image acquisition component;
and the second determination submodule is used for obtaining a standard central imaging position according to the central imaging position of the reference object under each rotation angle α.
In a possible implementation manner, the angle adjusting module 101 is further configured to sequentially adjust the rotating arm to a plurality of rotation angles β;
the position adjustment module 104 is further configured to adjust the position of the sample chamber in a direction parallel to the rotation plane of the rotary arm and perpendicular to the rotary arm at each rotation angle β until the reference center imaging position coincides with the standard center imaging position.
In a possible implementation manner, the angle adjusting module 101 is further configured to adjust the rotating arm to a rotation angle θ;
the position adjusting module 104 is further configured to adjust the position of the sample bin in a direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at the rotation angle θ until the imaging position of the center of the reference object coincides with the imaging position of the standard center;
the angle adjusting module 101 is further configured to adjust the rotating arm to a rotation angle phi perpendicular to the rotation angle theta;
the position adjusting module 104 is further configured to adjust the position of the sample chamber in a direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at the rotation angle phi until the imaging position of the center of the reference object coincides with the imaging position of the standard center.
In a possible implementation manner, the wide-angle light scattering device further comprises a laser, a sample to be detected is placed in the center of the sample bin, and laser emitted by the laser irradiates the sample bin;
the angle adjusting module 101 is further configured to adjust the rotating arm within a preset rotating angle range;
the acquisition module 102 is further configured to control the image acquisition component to acquire a plurality of images of the measured sample within a preset angle range, where the preset rotation angle range includes an initial 0 degree;
the apparatus 100 further comprises:
the second determining module is used for determining a plurality of optical signal intensity values corresponding to the standard center imaging position according to a plurality of images of the detected sample collected within a preset rotation angle range;
the third determining module is used for determining a rotation angle corresponding to the maximum optical signal intensity value in the plurality of optical signal intensity values;
and the fourth determining module is used for determining the standard zero rotation angle corresponding to the rotating arm according to the rotation angle corresponding to the maximum optical signal strength value.
In one possible implementation, the fourth determining module includes:
the third determining submodule is used for determining the initial 0 degree as a standard zero rotation angle if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is smaller than a preset threshold value;
and the fourth determining submodule is used for determining the rotation angle corresponding to the maximum optical signal intensity value as the standard zero rotation angle if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is greater than or equal to a preset threshold value.
Having described embodiments of the present disclosure, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The terms used herein were chosen in order to best explain the principles of the embodiments, the practical application, or technical improvements to the techniques in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A centering calibration method is applied to a wide-angle light scattering device, the wide-angle light scattering device comprises a sample bin, a rotating arm and an image acquisition component, the sample bin is located at one end close to the rotating center of the rotating arm, the image acquisition component is installed at one end, far away from the rotating center, of the rotating arm, a reference object is arranged in the sample bin, and the center of the reference object is coincident with the center of the sample bin;
the method comprises the following steps:
sequentially adjusting the rotating arm to a plurality of rotation angles α, and controlling the image acquisition component to acquire the image of the reference object at each rotation angle α;
determining a standard center imaging position from the images of the reference object acquired at the plurality of rotation angles α, wherein the standard center imaging position is an imaging position of the rotation center in the images acquired by the image acquisition component;
and adjusting the position of the sample bin according to the standard center imaging position.
2. The method of claim 1, wherein determining a standard center imaging position from the images of the reference acquired at the plurality of rotation angles α comprises:
determining a reference center imaging position at the rotation angle α according to the image of the reference acquired at any rotation angle α, wherein the reference center imaging position is the imaging position of the center of the reference in the image acquired by the image acquisition component;
and obtaining the standard central imaging position according to the central imaging position of the reference object at each rotation angle α.
3. The method of claim 2, wherein adjusting the position of the sample bin based on the isocenter imaging position comprises:
sequentially adjusting the rotating arm to a plurality of rotation angles β;
adjusting the position of the sample compartment in a direction parallel to the plane of rotation of the rotary arm and perpendicular to the rotary arm at each rotation angle β until the reference center imaging position coincides with the standard center imaging position.
4. The method of claim 2, wherein adjusting the position of the sample bin based on the isocenter imaging position comprises:
adjusting the rotating arm to a rotation angle theta;
adjusting the position of the sample bin in a direction which is parallel to the rotation plane of the rotating arm and is vertical to the rotating arm at a rotation angle theta until the central imaging position of the reference object coincides with the standard central imaging position;
adjusting the rotating arm to a rotating angle phi perpendicular to the rotating angle theta;
and adjusting the position of the sample bin in a direction which is parallel to the rotating plane of the rotating arm and is vertical to the rotating arm under the rotating angle phi until the central imaging position of the reference object coincides with the standard central imaging position.
5. The method according to claim 1, wherein the wide-angle light scattering device further comprises a laser, the sample to be measured is placed in the center of the sample chamber, and laser emitted by the laser irradiates the sample chamber;
the method further comprises the following steps:
adjusting the rotating arm within a preset rotating angle range, and controlling the image acquisition component to acquire a plurality of images of the tested sample within the preset angle range, wherein the preset rotating angle range comprises an initial 0 degree;
determining a plurality of light signal intensity values corresponding to the standard center imaging position according to a plurality of images of the detected sample collected within the preset rotation angle range;
determining a rotation angle corresponding to a maximum light signal intensity value in the plurality of light signal intensity values;
and determining a standard zero rotation angle corresponding to the rotary arm according to the rotation angle corresponding to the maximum optical signal intensity value.
6. The method according to claim 5, wherein determining a standard zero rotation angle for the rotary arm according to the rotation angle corresponding to the maximum optical signal strength value comprises one of:
if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is smaller than a preset threshold value, determining the initial 0 degree as the standard zero rotation angle;
and if the angle difference between the rotation angle corresponding to the maximum optical signal intensity value and the initial 0 degree is greater than or equal to the preset threshold value, determining the rotation angle corresponding to the maximum optical signal intensity value as the standard zero rotation angle.
7. A centering calibration device is characterized in that the device is applied to wide-angle light scattering equipment, the wide-angle light scattering equipment comprises a sample bin, a rotating arm and an image acquisition component, the sample bin is located at one end close to the rotating center of the rotating arm, the image acquisition component is installed at one end, far away from the rotating center, of the rotating arm, a reference object is arranged in the sample bin, and the center of the reference object is coincided with the center of the sample bin;
the device comprises:
an angle adjusting module, configured to sequentially adjust the rotating arm to a plurality of rotation angles α;
an acquisition module for controlling the image acquisition component to acquire an image of the reference object at each rotation angle α;
a first determination module that determines a standard center imaging position from the images of the reference object acquired at the plurality of rotation angles α, wherein the standard center imaging position is an imaging position of the rotation center in the image acquired by the image acquisition component;
and the position adjusting module is used for adjusting the position of the sample bin according to the standard central imaging position.
8. The apparatus of claim 7, wherein the first determining module comprises:
a first determining submodule, configured to determine a reference object center imaging position at any rotation angle α from the image of the reference object acquired at any rotation angle α, wherein the reference object center imaging position is an imaging position of the center of the reference object in the image acquired by the image acquisition component;
and the second determining submodule is used for obtaining the standard central imaging position according to the central imaging position of the reference object under each rotation angle α.
9. The apparatus of claim 8,
the angle adjusting module is further configured to sequentially adjust the rotating arm to a plurality of rotation angles β;
the position adjusting module is further configured to adjust the position of the sample chamber in a direction parallel to the rotation plane of the rotating arm and perpendicular to the rotating arm at each rotation angle β until the reference center imaging position coincides with the standard center imaging position.
10. The apparatus according to claim 7, further comprising a laser in the wide-angle light scattering device, wherein the sample to be measured is placed in the center of the sample chamber, and the laser emitted by the laser irradiates the sample chamber;
the angle adjusting module is also used for adjusting the rotating arm within a preset rotating angle range;
the acquisition module is further configured to control the image acquisition component to acquire a plurality of images of the sample to be detected within the preset angle range, where the preset rotation angle range includes an initial 0 degree;
the device further comprises:
the second determining module is used for determining a plurality of optical signal intensity values corresponding to the standard center imaging position according to a plurality of images of the detected sample acquired within the preset rotation angle range;
a third determining module, configured to determine a rotation angle corresponding to a maximum light signal intensity value of the plurality of light signal intensity values;
and the fourth determining module is used for determining a standard zero rotation angle corresponding to the rotating arm according to the rotation angle corresponding to the maximum optical signal strength value.
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