CN111253594B - 一种用于蛋白酶酶谱法的原位凝胶制备方法 - Google Patents
一种用于蛋白酶酶谱法的原位凝胶制备方法 Download PDFInfo
- Publication number
- CN111253594B CN111253594B CN202010197858.7A CN202010197858A CN111253594B CN 111253594 B CN111253594 B CN 111253594B CN 202010197858 A CN202010197858 A CN 202010197858A CN 111253594 B CN111253594 B CN 111253594B
- Authority
- CN
- China
- Prior art keywords
- gel
- prepared
- solution
- soil
- protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/16—Cyclodextrin; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2489/00—Characterised by the use of proteins; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Polymers & Plastics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
明胶浓度(w/v) | 灰度值 | |
实施例1 | 0.08% | 39 |
对比例1 | 0.06% | 47 |
对比例2 | 0.04% | 56 |
对比例3 | 0.02% | 92 |
对比例4 | 0% | 176 |
对比例5 | 0% | 33 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010197858.7A CN111253594B (zh) | 2020-03-19 | 2020-03-19 | 一种用于蛋白酶酶谱法的原位凝胶制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010197858.7A CN111253594B (zh) | 2020-03-19 | 2020-03-19 | 一种用于蛋白酶酶谱法的原位凝胶制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111253594A CN111253594A (zh) | 2020-06-09 |
CN111253594B true CN111253594B (zh) | 2021-03-02 |
Family
ID=70941952
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010197858.7A Active CN111253594B (zh) | 2020-03-19 | 2020-03-19 | 一种用于蛋白酶酶谱法的原位凝胶制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111253594B (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62297A (ja) * | 1985-06-25 | 1987-01-06 | Fuji Photo Film Co Ltd | 多層分析要素 |
CN102174641A (zh) * | 2010-12-30 | 2011-09-07 | 中国科学院南京土壤研究所 | 一种测定碱性蛋白酶活力的酶谱方法 |
CN108467498A (zh) * | 2018-06-12 | 2018-08-31 | 华中科技大学 | 一种壳聚糖和明胶的超分子水凝胶及其制备方法与应用 |
CN108693149A (zh) * | 2017-04-06 | 2018-10-23 | 中国科学院地理科学与资源研究所 | 一种用于测定野外根际土壤原位酶谱的设备及方法 |
CN109748636A (zh) * | 2017-11-02 | 2019-05-14 | 单维民 | 一种提高营养元素利用率的有机蛋白酶 |
-
2020
- 2020-03-19 CN CN202010197858.7A patent/CN111253594B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS62297A (ja) * | 1985-06-25 | 1987-01-06 | Fuji Photo Film Co Ltd | 多層分析要素 |
CN102174641A (zh) * | 2010-12-30 | 2011-09-07 | 中国科学院南京土壤研究所 | 一种测定碱性蛋白酶活力的酶谱方法 |
CN108693149A (zh) * | 2017-04-06 | 2018-10-23 | 中国科学院地理科学与资源研究所 | 一种用于测定野外根际土壤原位酶谱的设备及方法 |
CN109748636A (zh) * | 2017-11-02 | 2019-05-14 | 单维民 | 一种提高营养元素利用率的有机蛋白酶 |
CN108467498A (zh) * | 2018-06-12 | 2018-08-31 | 华中科技大学 | 一种壳聚糖和明胶的超分子水凝胶及其制备方法与应用 |
Non-Patent Citations (1)
Title |
---|
Incorporation of fluorescent enzyme substrates in agarose gel for in situ zymography;Chin-Feng Yi et al.;《ANALYTICAL BIOCHEMISTRY》;20010226;第291卷(第1期);第27-33页 * |
Also Published As
Publication number | Publication date |
---|---|
CN111253594A (zh) | 2020-06-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Demura et al. | Immobilization of glucose oxidase with Bombyx mori silk fibroin by only stretching treatment and its application to glucose sensor | |
Hodgson et al. | Biosensors for characterizing the dynamics of rho family GTPases in living cells | |
Biró et al. | A substance effecting differentiation in Streptomyces griseus: purification and properties | |
CN111253594B (zh) | 一种用于蛋白酶酶谱法的原位凝胶制备方法 | |
Yasui et al. | Invasive growth of Aspergillus oryzae in rice koji and increase of nuclear number | |
Wisman et al. | Mapping haze-komi on rice koji grains using β-glucuronidase expressing Aspergillus oryzae and mass spectrometry imaging | |
Devkota et al. | FRAP: a powerful method to evaluate membrane fluidity in Caenorhabditis elegans | |
CN103621308B (zh) | 细脚棒束孢子实体生产的培养基及工业化培养方法 | |
Liu et al. | Scale high-cell-density fermentation of Pichia pastoris | |
CN112710644B (zh) | 测定水生植物根际磷酸酶活性二维空间分布的酶谱方法 | |
Ito et al. | Quantitative evaluation of haze formation of koji and progression of internal haze by drying of koji during koji making | |
Newton et al. | Process adapted calibration improves fluorometric pH sensor precision in sophisticated fermentation processes | |
TWI700371B (zh) | 含玻尿酸生物纖維材料的製備方法 | |
Tanino et al. | Visualization of polypeptides including fragmented α-amylase in rice koji grains using mass spectrometry imaging | |
Wurm et al. | Efficient development of a mixed feed process for Pichia pastoris | |
Atha et al. | Cellular reference materials for DNA damage using electrochemical oxidation | |
CN117126874B (zh) | 大规模制备164.88°三螺旋结构胶原蛋白生物方法 | |
CN109996805A (zh) | 蛋白质组合物、其制造方法和热稳定性提高方法 | |
CN115109743B (zh) | 一种复合丝素蛋白仿生类肝小叶支架及其制备方法与应用 | |
CN117551113A (zh) | 一种1,2,3-三氮唑-生物素分子探针及其制备与应用 | |
CN110760466B (zh) | 一株产耐高温中性蛋白酶的枯草芽孢杆菌及其应用 | |
CN113150334B (zh) | 一种硝酸纤维素膜制备工艺 | |
Tee et al. | A cryostat method for the extraction of soluble tissue components | |
Gmeiner et al. | Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode | |
SU542503A1 (ru) | Способ получени гидролизатов из протеинсодержащего сырь |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Qian Jin Inventor after: Liu Yin Inventor after: Lu Bianhe Inventor after: Zhao Yuanyuan Inventor after: Qian Xinyuan Inventor after: Shen Junwei Inventor after: Zhu Xinze Inventor before: Liu Yin Inventor before: Qian Jin Inventor before: Lu Bianhe Inventor before: Zhao Yuanyuan Inventor before: Qian Xinyuan Inventor before: Shen Junwei Inventor before: Zhu Xinze |
|
GR01 | Patent grant | ||
GR01 | Patent grant |