CN111249408A

CN111249408A - Swelling-diminishing gel and preparation method thereof

Info

Publication number: CN111249408A
Application number: CN202010243573.2A
Authority: CN
Inventors: 罗华云; 张京平
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-31
Filing date: 2020-03-31
Publication date: 2020-06-09

Abstract

The invention discloses a swelling-diminishing gel and a preparation method thereof, wherein the swelling-diminishing gel is prepared from the following raw materials in parts by weight: 1000 parts of 500-plus traditional Chinese medicine extract, 50-190 parts of carbomer, 100-plus 2000 parts of glycerin, 5-20 parts of ethyl p-carboxymethylbenzoate and 30-200 parts of triethanolamine; the traditional Chinese medicine extract is extracted from the following components in parts by weight: 5-15 parts of erythrina bark, 8-18 parts of angelica, 3-13 parts of peach kernel, 3-13 parts of safflower, 6-16 parts of frankincense, 6-16 parts of myrrh, 9-19 parts of radix stephaniae tetrandrae, 10-20 parts of coix seed, 3-13 parts of pericarpium zanthoxyli and 5-15 parts of liquorice utilize the effective components of the traditional Chinese medicines, have the effects of promoting blood circulation to remove blood stasis and relieving swelling and pain, the added auxiliary agent is safe and nontoxic, and can not cause skin red swelling, pruritus and the like, and the preparation method is simple to operate.

Description

Swelling-diminishing gel and preparation method thereof

Technical Field

The invention relates to the technical field of external traditional Chinese medicine preparations, in particular to a swelling-diminishing gel and a preparation method thereof.

Background

At present, synovitis and arthralgia are diseases which are not classified by age and sex, the disease condition is complex and continuous, so that the joint of people is swollen, the movement of people is difficult, and the daily life, physical and mental health of people are seriously affected. The original patient mostly uses the decocted liquid medicine to fumigate and wash the affected part; or the paste is smeared on the affected part, which can affect the life of the patient, and the use is inconvenient and the clothes are polluted. In addition, the existing external medicine for promoting blood circulation and removing blood stasis has large irritation to skin, is easy to generate allergic phenomena such as red swelling, pruritus and the like, cannot achieve unobvious treatment effect, and cannot effectively relieve symptoms such as joint swelling, joint pain and the like.

Therefore, the research of a swelling-reducing gel with convenient use, definite curative effect and small toxic and side effects and a preparation method thereof through modern means and related pharmaceutical knowledge is a problem which needs to be solved urgently by the technical personnel in the field.

Disclosure of Invention

In view of the above, the invention provides a swelling-reducing gel and a preparation method thereof, which utilize the effective components of traditional Chinese medicines, have the effects of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, are safe and nontoxic in added auxiliary agents, do not cause skin red swelling, pruritus and the like, and are simple to operate.

In order to achieve the purpose, the invention adopts the following technical scheme:

the swelling-diminishing gel is prepared from the following raw materials in parts by weight: 1000 parts of 500-plus traditional Chinese medicine extract, 50-190 parts of carbomer, 100-plus 2000 parts of glycerin, 5-20 parts of ethyl p-carboxymethylbenzoate and 30-200 parts of triethanolamine; the traditional Chinese medicine extract is obtained by extracting the following components in parts by weight: 5-15 parts of erythrina bark, 8-18 parts of angelica, 3-13 parts of peach kernel, 3-13 parts of safflower, 6-16 parts of frankincense, 6-16 parts of myrrh, 9-19 parts of radix stephaniae tetrandrae, 10-20 parts of coix seed, 3-13 parts of pericarpium zanthoxyli and 5-15 parts of liquorice.

The beneficial effects of the preferred technical scheme are as follows: the Chinese medicinal composition is prepared by extracting medicinal materials such as radix sophorae flavescentis and safflower to obtain important extracts, and plays a role in compatibility among the important materials, so that multiple effects of clearing heat and drying dampness, promoting blood circulation and dredging channels, removing blood stasis and relieving pain and the like are achieved, and pain and symptoms of diseases are effectively relieved; in addition, the swelling-diminishing gel takes carbomer as a gel, glycerin and triethanolamine as moisturizers to play a moisturizing role, and the swelling-diminishing gel is matched with the traditional Chinese medicine extract to obtain the swelling-diminishing gel convenient to use.

The invention also provides a preparation method of the swelling-diminishing gel, which comprises the following steps:

(1) weighing the raw materials according to the swelling-reducing gel for later use;

(2) decocting the Chinese medicinal materials, filtering, and concentrating to obtain Chinese medicinal extract;

(3) dissolving carbomer in water to obtain carbomer water solution; dissolving ethyl p-carboxymethylbenzoate in ethanol to obtain a solution of ethyl p-carboxymethylbenzoate;

(4) heating the Chinese medicinal extract, adding into carbomer water solution, and stirring; then adding glycerol and stirring uniformly; adding the ethyl p-carboxymethylbenzoate solution and stirring uniformly; finally adding triethanolamine and stirring uniformly to obtain the swelling-reducing gel.

The beneficial effects of the preferred technical scheme are as follows: the preparation method disclosed by the invention avoids the complex preparation process of the traditional Chinese medicine, overcomes the defects that the traditional ointment is easy to stain clothes and cause inconvenience when in use, is convenient to use, is not greasy, is easy to absorb, has good spreadability and avoids adverse reaction of oral administration. Accords with various standards and parameters under the item of the nucleus < Chinese pharmacopoeia > gel.

Preferably, the step (2) specifically comprises:

(a) adding water into the raw materials, decocting, and filtering to obtain a first decoction dregs and a first decoction respectively;

(b) adding water into the first decoction dregs, decocting, and filtering to obtain a second decoction dregs and a second liquid medicine respectively;

(c) adding water into the second decoction dregs, decocting, and filtering to obtain a third decoction dregs and a third liquid medicine respectively;

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain the traditional Chinese medicine extract.

The beneficial effects of the preferred technical scheme are as follows: the invention can efficiently extract the effective components in the traditional Chinese medicine raw materials and improve the utilization rate of the raw materials through multiple times of decoction and filtration in the preparation process of the traditional Chinese medicine extract.

Preferably, water is added in the step (a) according to the solid-to-liquid ratio of 228:1600g/mL, and the decocting time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

Preferably, water is added in the step (b) according to the solid-to-liquid ratio of 228:1500g/mL, and the decocting time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

Preferably, water is added in the step (c) according to the solid-to-liquid ratio of 228:1500g/mL, and the decocting time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

Preferably, the filtering in the step (d) adopts a 80-200 mesh sieve, and the concentration adopts a traditional Chinese medicine concentration pot to concentrate until the density of the traditional Chinese medicine extract is 1-1.7g/mL and the crude drug amount is 1-10 g/mL.

Preferably, the mass concentration of the carbomer aqueous solution in the step (3) is 0.0214 g/mL; the mass concentration of the ethanol is 95%, and the concentration of the ethyl p-carboxymethylbenzoate solution is 0.108 g/mL.

Preferably, the stirring in the step (4) is performed by a high-speed stirrer, and the stirring speed is 1000 r/min.

According to the technical scheme, compared with the prior art, the swelling-diminishing gel and the preparation method thereof disclosed by the invention have the following beneficial effects:

(1) the traditional Chinese medicine effective components are utilized, the effects of promoting blood circulation to remove blood stasis and relieving swelling and pain are achieved, the added auxiliary agent is safe and non-toxic, red swelling, pruritus and the like of the skin cannot be caused, and the preparation method is simple to operate;

(2) the swelling-diminishing gel with excellent use performance is obtained by using carbomer as a gel, using glycerin and triethanolamine as moisturizers to play the roles of moisturizing and skin and mutually matching with the traditional Chinese medicine extract;

(3) the preparation method disclosed by the invention is simple to operate and can be industrially popularized, and the prepared swelling-reducing gel has the advantages of no precipitation, fine texture, gel retention, no agglomeration or liquefaction, and excellent spreadability, stability and glossiness.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a thin layer chromatogram of Glycyrrhiza uralensis according to the present invention;

FIG. 2 is a thin layer chromatogram of Sophora flavescens ait provided by the present invention;

FIG. 3 is a thin layer chromatogram of safflower according to the invention;

FIG. 4 is a thin layer chromatogram of Angelica sinensis provided by the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The embodiment of the invention provides a swelling-diminishing gel which is prepared from the following raw materials in parts by weight: 1000 parts of 500-plus traditional Chinese medicine extract, 50-190 parts of carbomer, 100-plus 2000 parts of glycerin, 5-20 parts of ethyl p-carboxymethylbenzoate and 30-200 parts of triethanolamine; the traditional Chinese medicine extract is obtained by extracting the following components in parts by weight: 5-15 parts of erythrina bark, 8-18 parts of angelica, 3-13 parts of peach kernel, 3-13 parts of safflower, 6-16 parts of frankincense, 6-16 parts of myrrh, 9-19 parts of radix stephaniae tetrandrae, 10-20 parts of coix seed, 3-13 parts of pericarpium zanthoxyli and 5-15 parts of liquorice. .

The embodiment of the invention also provides a preparation method of the swelling-diminishing gel, which comprises the following steps:

(2) extracting the Chinese medicinal extract

(a) Adding water into the traditional Chinese medicine raw materials, decocting, and filtering to obtain a first decoction dregs and a first decoction respectively; adding water according to the solid-to-liquid ratio of 228:1600g/mL, and decocting for 0.5-1 h; filtering with 80-200 mesh sieve;

(b) adding water into the first decoction dregs, decocting, and filtering to obtain a second decoction dregs and a second liquid medicine respectively; adding water according to the solid-to-liquid ratio of 228:1500g/mL, and decocting for 0.5-1 h; filtering with 80-200 mesh sieve;

(c) adding water into the second decoction dregs, decocting, and filtering to obtain a third decoction dregs and a third liquid medicine respectively; adding water according to the solid-to-liquid ratio of 228:1500g/mL, and decocting for 0.5-1 h; filtering with 80-200 mesh sieve;

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain a traditional Chinese medicine extract; filtering with 80-200 mesh sieve, concentrating with Chinese medicinal concentrating pan until the density of Chinese medicinal extract is 1-1.7g/mL and the crude drug amount is 1-10 g/mL;

(3) dissolving carbomer in water to obtain carbomer water solution; dissolving ethyl p-carboxymethylbenzoate in ethanol to obtain a solution of ethyl p-carboxymethylbenzoate; the mass concentration of the carbomer aqueous solution is 0.0214 g/mL; the mass concentration of the ethanol is 95 percent, and the concentration of the ethyl p-carboxymethylbenzoate solution is 0.108 g/mL;

(4) heating the Chinese medicinal extract, adding into carbomer water solution, and stirring; then adding glycerol and stirring uniformly; adding the ethyl p-carboxymethylbenzoate solution and stirring uniformly; finally adding triethanolamine and stirring uniformly to obtain the swelling-reducing gel; wherein the stirring is carried out by a high-speed stirrer, and the stirring speed is 1000 r/min.

Example 1

The embodiment 1 of the invention provides a swelling-diminishing gel, which is prepared from the following raw materials in parts by weight: 900g of Chinese medicinal extract, 135g of carbomer, 900g of glycerol, 10.8g of ethyl p-carboxymethylbenzoate and 135g of triethanolamine; the traditional Chinese medicine extract is extracted from the following traditional Chinese medicine raw materials in parts by weight: 15 parts of erythrina bark, 18 parts of angelica, 13 parts of peach kernel, 13 parts of safflower, 16 parts of frankincense, 16 parts of myrrh, 19 parts of radix stephaniae tetrandrae, 20 parts of coix seed, 13 parts of pericarpium zanthoxyli and 15 parts of liquorice.

The embodiment 1 of the invention also provides a preparation method of the swelling-diminishing gel, which specifically comprises the following steps:

(1) weighing 135g of carbomer, 900g of glycerol, 10.8g of ethyl p-carboxymethylbenzoate, 135g of triethanolamine and 2212g of total weight of traditional Chinese medicine raw materials for later use;

(2) extracting the Chinese medicinal extract

(a) Adding 16000mL of water into the traditional Chinese medicine raw materials, decocting for 1h, and filtering to obtain a first medicine residue and a first medicine liquid respectively;

(b) adding 15000mL of water into the first decoction dregs, decocting for 1h, and filtering to obtain a second decoction dregs and a second liquid medicine respectively;

(c) adding 15000mL of water into the second medicinal residue, decocting for 1h, and filtering to obtain a third medicinal residue and a third medicinal liquid respectively;

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain a traditional Chinese medicine extract; the density of the traditional Chinese medicine extract is 1.23g/mL, and the crude drug amount is 3.26 g/mL; wherein, the concentration adopts a traditional Chinese medicine concentration pot;

(3) dissolving carbomer in water to obtain 0.0214g/mL carbomer aqueous solution; dissolving ethyl p-carboxymethylbenzoate in ethanol to obtain a solution of ethyl p-carboxymethylbenzoate with the concentration of 0.108 g/mL; the mass concentration of the ethanol is 95 percent;

(4) heating the Chinese medicinal extract, adding into carbomer water solution, and stirring; then adding glycerol and stirring uniformly; adding the ethyl p-carboxymethylbenzoate solution and stirring uniformly; finally adding triethanolamine and stirring uniformly to obtain the swelling-reducing gel; wherein the stirring is carried out by a high-speed stirrer at the stirring speed of 1000 r/min.

Example 2

The embodiment 2 of the invention provides a swelling-diminishing gel, which is prepared from the following raw materials in parts by weight: 500g of traditional Chinese medicine extract, 50g of carbomer, 100g of glycerol, 5g of ethyl p-carboxymethylbenzoate and 30g of triethanolamine; the traditional Chinese medicine extract is extracted from the following traditional Chinese medicine raw materials in parts by weight: 5 parts of erythrina bark, 8 parts of angelica, 3 parts of peach kernel, 3 parts of safflower, 6 parts of frankincense, 6 parts of myrrh, 9 parts of radix stephaniae tetrandrae, 10 parts of coix seed, 3 parts of pericarpium zanthoxyli and 5 parts of liquorice.

The embodiment 2 of the invention also provides a preparation method of the swelling-diminishing gel, which specifically comprises the following steps:

(1) weighing 50g of carbomer, 100g of glycerol, 5g of ethyl p-carboxymethylbenzoate, 30g of triethanolamine and 2320g of traditional Chinese medicine raw materials for later use according to the swelling relieving gel;

(2) extracting the Chinese medicinal extract

(a) Adding 16000mL of water into the traditional Chinese medicine raw materials, decocting for 0.5h, and filtering to obtain a first medicine residue and a first medicine liquid respectively;

(b) adding 15000mL of water into the first medicinal residue, decocting for 0.5h, and filtering to obtain a second medicinal residue and a second medicinal liquid respectively;

(c) adding 15000mL of water into the second medicinal residue, decocting for 0.5h, and filtering to obtain a third medicinal residue and a third medicinal liquid respectively;

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain a traditional Chinese medicine extract; the density of the traditional Chinese medicine extract is 1.5g/mL, and the crude drug amount is 3.50 g/mL; wherein, the concentration adopts a traditional Chinese medicine concentration pot;

Example 3

The embodiment 3 of the invention provides a swelling-diminishing gel, which is prepared from the following raw materials in parts by weight: 700g of Chinese medicinal extract, 60g of carbomer, 1500g of glycerol, 14g of ethyl p-carboxymethylbenzoate and 40g of triethanolamine; the traditional Chinese medicine extract is extracted from the following traditional Chinese medicine raw materials in parts by weight: 10 parts of erythrina bark, 13 parts of angelica, 8 parts of peach kernel, 8 parts of safflower, 11 parts of frankincense, 11 parts of myrrh, 14 parts of radix stephaniae tetrandrae, 15 parts of semen coicis, 8 parts of pericarpium zanthoxyli and 10 parts of liquorice.

The embodiment 3 of the invention also provides a preparation method of the swelling-diminishing gel, which specifically comprises the following steps:

(1) weighing 60g of carbomer, 1500g of glycerol, 14g of ethyl p-carboxymethylbenzoate, 40g of triethanolamine and 2268g of traditional Chinese medicine raw materials in total weight for later use according to the swelling diminishing gel;

(2) extracting the Chinese medicinal extract

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain a traditional Chinese medicine extract; the density of the traditional Chinese medicine extract is 1.6g/mL, and the crude drug amount is 3.50 g/mL; wherein, the concentration adopts a traditional Chinese medicine concentration pot;

(4) heating the Chinese medicinal extract, adding into carbomer water solution, and stirring; then adding glycerol and stirring uniformly; adding the ethyl p-carboxymethylbenzoate solution and stirring uniformly; and finally adding triethanolamine and stirring uniformly to obtain the swelling-reducing gel, wherein the stirring is carried out by adopting a high-speed stirrer at the stirring speed of 1000 r/min.

Example 4

The embodiment 4 of the invention provides a swelling-diminishing gel, which is prepared from the following raw materials in parts by weight: 800g of Chinese medicinal extract, 180g of carbomer, 200g of glycerol, 6g of ethyl p-carboxymethylbenzoate and 30g of triethanolamine; the traditional Chinese medicine extract is extracted from the following traditional Chinese medicine raw materials in parts by weight: 6 parts of erythrina bark, 8 parts of angelica, 3 parts of peach kernel, 13 parts of safflower, 16 parts of frankincense, 16 parts of myrrh, 19 parts of radix stephaniae tetrandrae, 20 parts of coix seed, 10 parts of pericarpium zanthoxyli and 15 parts of liquorice.

The embodiment 4 of the invention also provides a preparation method of the swelling-diminishing gel, which specifically comprises the following steps:

(1) weighing 180g of carbomer, 200g of glycerol, 6g of ethyl p-carboxymethylbenzoate, 30g of triethanolamine and 2268g of traditional Chinese medicine raw materials in total weight for later use;

(2) extracting the Chinese medicinal extract

(d) mixing the first liquid medicine, the second liquid medicine and the third liquid medicine, filtering and concentrating to obtain a traditional Chinese medicine extract; the density of the traditional Chinese medicine extract is 1.6g/mL, and the crude drug amount is 3.5 g/mL; wherein, the concentration adopts a traditional Chinese medicine concentration pot;

Effect verification

First, verification standard

According to the specification of 'Chinese pharmacopoeia' 2015 edition, the gel has no precipitation and fine texture at normal temperature, keeps gel state, does not cake or liquefy, and examines the forming condition of the gel by looking up relevant data documents and taking the formability, spreadability, stability, glossiness, water loss rate and viscosity of the gel as indexes.

II, stability

(1) Centrifugal experiment

After the gel prepared by the embodiment of the invention is centrifuged for 30min at 3000r/min, the layering, caking and other appearance changes of the gel are observed, and the results are shown in the following table 1.

(2) Low temperature experiment

The gel prepared in the example of the present invention was placed in a refrigerator at (-20 ℃) for 24 hours, and delamination, caking and other appearance changes of the gel were observed, and the results are shown in table 1 below.

(3) Thermal constant temperature test

The gel prepared in example was placed in a water bath at 55 ℃ for 6 hours, and the delamination, caking and other appearance changes of the gel were observed, and the results are shown in Table 1 below.

TABLE 1

The results in table 1 clearly show that the gel prepared in example 1 of the present invention has no delamination, uniform distribution, no impurities, and no apparent change after centrifugation, low temperature, and constant temperature tests, which indicates that the gel prepared in the present invention has excellent stability. Examples 2, 3, 4 are less stable.

Third, viscosity measurement

The viscosity of the gel prepared in each example was measured at 17 ℃ using a rotational viscometer of type NDJ-1 with a corresponding rotor and rotation speed, and the results are shown in Table 2 below.

Fourth, measuring the water loss rate

Placing appropriate amount of gel into a culture dish, weighing, drying in oven at 55 deg.C, taking out every 1 hr, cooling, and weighing again until the gel mass does not change to the end point. The water loss rate was calculated according to the following formula, and the obtained results are shown in table 2 below.

TABLE 2

	Viscosity of the solution	Water loss rate/%)
			Example 1	100Pa.s	98.27％
Example 2	108Pa.s	89..25％
			Example 3	99Pa.s	98.31％
Example 4	89Pa.s	97.71％

As is apparent from the data in table 2, the viscosity value of the swelling-reducing gel preparation prepared by the present invention is 100pa.s, which meets the regulations; the water loss rate of the swelling-reducing gel prepared by the invention is 98.27%.

Fifth, experiment on antiseptics

After the swelling-reducing gel prepared in the example is placed for one month, the swelling-reducing gel is observed to have no mildew phenomenon.

Sixth, detection standard

1. Traits

The swelling-reducing gel prepared in each example was applied to a petri dish, which was white and transparent, and the appearance thereof was observed as shown in table 3 below.

TABLE 3

From the above results it is evident that: example 1 three batches of samples were randomly sampled and tested to have the appearance shape and character meeting the specification.

2. pH value

Adding 2g of gel into 40ml of distilled water, performing ultrasonic treatment for 10min, and measuring the pH value by using a pH acidity meter after the gel is dissolved. The pH values are as follows:

TABLE 4

From the results of table 4 above, it can be seen that: the pH value measured by a pH acidity meter is in the range of 4.5-6.5; and the pH value of the skin surface is met.

Seventh, qualitative detection

1. Qualitative identification of licorice

Dissolving in 100ml of methanol10g of the gel prepared in example 1 is subjected to ultrasonic treatment for 30min, then filtered, the filtrate is evaporated to dryness, and then dissolved in 30ml of water, the extraction is carried out twice by using ethyl acetate, the evaporation is carried out to dryness, 1ml of methanol is added into the residue to obtain a sample solution, a negative control solution is prepared by the same method, 2g of a licorice control drug is taken and put into 100ml of water, the decoction is carried out for 30min, then the filtration is carried out, and the concentration is carried out until the concentration is 30ml^[13](ii) a Sucking three solutions of 5 μ l each, dropping on silica gel G thin layer plate prepared from 1% sodium hydroxide solution, developing with ethyl acetate-formic acid-glacial acetic acid-water (17: 1: 1: 1.5) as developing agent, taking out, air drying, spraying 10% sulfuric acid ethanol solution, heating to 105 deg.C, allowing spots to develop clearly, viewing under ultraviolet lamp, and allowing the negative control medicinal material solution to have no fluorescent spot at the position corresponding to the control medicinal material, as shown in FIG. 1.

2. Qualitative identification of Sophora flavescens

Dissolving 10g of the gel prepared in the embodiment 1 in 100ml of methanol, performing ultrasonic filtration for 30min, evaporating the filtrate to dryness, dissolving in 50ml of water, adding concentrated ammonia solution to adjust the pH value to be 9-10, extracting twice with 30ml of chloroform, evaporating to dryness, adding 1ml of methanol to obtain a sample solution, and preparing a negative control solution by the same method; collecting matrine, adding methanol to obtain 1mg/1ml reference solution^[14](ii) a Sucking three solutions of 5 μ l each, dropping on silica gel G thin layer plate of 2% sodium hydroxide solution, developing with toluene-ethyl acetate-acetone-concentrated ammonia solution (2: 4: 3: 0.2) as developing agent, taking out, air drying, spraying with improved bismuth potassium iodide solution, observing the position corresponding to control medicinal material, wherein the negative control medicinal material solution has no fluorescent spot, as shown in FIG. 2.

3. Qualitative identification of safflower

Dissolving 10g of the gel prepared in example 1 in 100ml of methanol, carrying out ultrasonic treatment for 30min, filtering, evaporating the filtrate, adding 30ml of water, extracting twice with ethyl acetate, shaking and extracting for 2 times with 20ml of 1% sodium hydroxide solution, adjusting the pH value to 5 with dilute hydrochloric acid, shaking and extracting twice with 30ml of ethyl acetate, dehydrating with anhydrous sodium sulfate, evaporating to dryness, adding 1ml of ethyl acetate to dissolve to obtain a sample solution, preparing a negative control solution by the same method, taking 1g of a safflower control medicinal material, adding a proper amount of water, decocting for 1h, cooling, filtering, concentrating to about 20ml^[15](ii) a Pipette 5. mu.l of each solution, and spot on each solutionSpreading on the same silica gel G thin layer plate with cyclohexane-ethyl acetate-glacial acetic acid (7:4:1) as developing agent, taking out, air drying, fumigating with strong ammonia bottle for 30min, and inspecting under ultraviolet lamp. Spots of the same color appear at the positions corresponding to the chromatogram of the control drug and the sample, as shown in FIG. 3.

4. Qualitative identification of Angelica sinensis

Dissolving 10g of the gel prepared in the embodiment 1 in 100Ml of methanol, carrying out ultrasonic treatment for 30min, filtering, evaporating filtrate, dissolving in 30Ml of water, shaking and extracting with diethyl ether for 2 times, evaporating, adding 1Ml of ethyl acetate into residues to obtain a sample solution, preparing a negative control solution by the same method, adding 1g of a Chinese angelica control medicinal material into 30Ml of ethyl ether solution, carrying out ultrasonic treatment for 10min, filtering, evaporating, adding 1Ml of ethyl acetate, dissolving to obtain a control medicinal material solution [16 ]; sucking three solutions of 5 μ l each, dropping on the same silica gel G thin layer plate, spreading with n-hexane-ethyl acetate (9:1) as developing agent, taking out, air drying, viewing under ultraviolet lamp, and displaying no clearly distinguished spots at the corresponding positions of the reference medicinal material and the sample, with the result as shown in FIG. 4.

As is evident from the results of the above-mentioned FIGS. 1 to 4, the purple spots in FIG. 1 are clearly distinguished, and the negative control is not interfered; FIG. 2 shows a clear yellow main spot as a distinction, with no interference in the negative control; FIG. 3 shows a clear spot as a distinction, with no interference in the negative control; FIG. 4 shows no obvious spots, and the specificity is not strong, so it is not suitable for qualitative herbs.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The swelling-diminishing gel is characterized by comprising the following raw materials in parts by weight: 1000 parts of 500-plus traditional Chinese medicine extract, 50-190 parts of carbomer, 100-plus 2000 parts of glycerin, 5-20 parts of ethyl p-carboxymethylbenzoate and 30-200 parts of triethanolamine; the traditional Chinese medicine extract is obtained by extracting the following components in parts by weight: 5-15 parts of erythrina bark, 8-18 parts of angelica, 3-13 parts of peach kernel, 3-13 parts of safflower, 6-16 parts of frankincense, 6-16 parts of myrrh, 9-19 parts of radix stephaniae tetrandrae, 10-20 parts of coix seed, 3-13 parts of pericarpium zanthoxyli and 5-15 parts of liquorice.

2. The preparation method of the swelling-diminishing gel is characterized by comprising the following steps:

(1) the swelling-reducing gel according to claim 1 is prepared by weighing raw materials for later use;

3. The preparation method of the swelling-diminishing gel according to claim 1, wherein the step (2) specifically comprises the following steps:

4. The preparation method of the swelling-reducing gel as claimed in claim 3, wherein water is added in the step (a) according to the solid-to-liquid ratio of 228:1600g/mL, and the decoction time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

5. The preparation method of the swelling-reducing gel as claimed in claim 3, wherein water is added in the step (b) according to the solid-to-liquid ratio of 228:1500g/mL, and the decoction time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

6. The preparation method of the swelling-diminishing gel as claimed in claim 3, wherein water is added in the step (c) according to the solid-to-liquid ratio of 228:1500g/mL, and the decoction time is 0.5-1 h; the filtration adopts a 80-200 mesh sieve.

7. The preparation method of the swelling-diminishing gel as claimed in claim 3, wherein the filtering in the step (d) is performed by using a 80-200 mesh sieve, and the concentration is performed by using a traditional Chinese medicine concentration pot until the density of the traditional Chinese medicine extract is 1-1.7g/mL and the crude drug amount is 1-10 g/mL.

8. The preparation method of the swelling-diminishing gel according to claim 2, wherein the mass concentration of the carbomer aqueous solution in the step (3) is 0.0214 g/mL; the mass concentration of the ethanol is 95%, and the concentration of the ethyl p-carboxymethylbenzoate solution is 0.108 g/mL.

9. The method for preparing the swelling-reducing gel agent according to claim 3, wherein the stirring in the step (4) is performed by a high-speed stirrer, and the stirring speed is 1000 r/min.