CN1112394A - Preparation method for cow foremilk bio-active products - Google Patents
Preparation method for cow foremilk bio-active products Download PDFInfo
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- CN1112394A CN1112394A CN 95100461 CN95100461A CN1112394A CN 1112394 A CN1112394 A CN 1112394A CN 95100461 CN95100461 CN 95100461 CN 95100461 A CN95100461 A CN 95100461A CN 1112394 A CN1112394 A CN 1112394A
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- casein
- emulsion
- sterilization
- immunoglobulin
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Abstract
The preparation method for bioactive products of colostrum milk includes following steps : removing fat and casein in colostrum milk by centrifugal separation, adding abomasin or pepsin or hydrochloric acid and removing the grume by centrifugal separation, then picking up immunoglobulin and lysozyme or precipitating the immunoglobulin by adding saturated ammonium sulfate, or removing the large molecular substances by hyperfiltration to obtain the product, which has notable effect for prevention and treatment of infant diarrhea and sensile chronic bronchitis.
Description
The present invention relates to a kind of colostrum is processed and handle, prepare the method for bio-active products.
Colostrum is meant secreted milk in the cow birth back 5 days.Colostrum contains extremely rich nutrient contents, and as proteins,vitamins,and minerals etc., especially multiple bioactive substance content such as immunoglobulin (Ig), lysozyme is higher.Colostrum has the effect that strengthens children and the elderly's immunity, can prevent and treat infantile diarrhea and senile chronic bronchitis effectively.
Colostrum has foreign odor, bitter, viscosity big, always is not eaten or is not used for the raw material that dairy products are produced.In order to make colostrum can be prepared into various useful nutriment, now existing many methods are studied out.As " refinement method of colostrum and edible colostrum powder thereof " (publication number 1043861), it is that former breast is checked and accepted, and the colostrum centrifugation is three layers after the preliminary treatment, the fat deposit that promptly condenses, emulsion, casein and tangible solids precipitation thing layer; Get the emulsion dried powderization and get product." colostrum drink production method " (publication number 1085734), it is that the colostrum after checking and accepting is purified colostrum then by required sugaring or other flavouring, nutriment with centrifuge, makes various dairy products just.Last two kinds of methods all adopt centrifugal layering to remove the casein of fat deposit and precipitation, though fat can be removed effectively, casein can only be removed about 10-20%.Caseic content height then is unfavorable for digesting and assimilating of infant and the elderly.
The objective of the invention is effectively to remove caseic present situation according to said method, aim to provide a kind of colostrum processing method that casein more than 90% can be removed, and produce the cow colostrum active nutrition product that helps infant and the elderly's absorption by this method.
The objective of the invention is to realize in the following manner, the cow foremilk bio-active products preparation method, its technological process is that former breast is checked and accepted, is three layers with centrifuge with 3000-4000 rev/min of rotating speed centrifugation, removes upper strata fat deposit and bottom casein particle layer, takes out middle emulsion.In emulsion, add rennin or pepsin or hydrochloric acid effect half an hour, to the visible grumeleuse of naked eyes occurring, be two-layer with centrifuge with 3000-4000 rev/min of rotating speed centrifugation again, remove the casein of precipitation, get supernatant with cobalt-60 radiation sterilization or under 62 ℃ of temperature bacterium 30 minutes, again with the ammonium sulfate method measure its immunoglobulin content (>30mg/ml), measure lysozyme content (>1 μ g/ml) with the reference culture method, be packaged as oral liquid or make the freeze drying product after qualified.
The present invention is after removing upper strata fat and a small amount of casein of lower floor with the colostrum centrifugation, in emulsion, add rennin or pepsin again, or to wherein adding HCl, casein in the emulsion will with the above-mentioned substance effect, being grumeleuse separates out, centrifugation more then can be removed the casein more than 90% wherein, helps digesting and assimilating of infant and the elderly.
Behind the centrifugal removal casein of emulsion, can act on 30 minutes to the saturated ammonium sulfate that wherein adds clear liquid amount 30-50%, the centrifuging and taking precipitation adds a small amount of dissolved in distilled water in precipitation, use the normal saline dialysis deionization again, promptly gets and concentrates the immunoglobulin (Ig) highly finished product.This product is more obvious to infantile diarrhea, senile chronic bronchitis prevention effect.
Behind the centrifugal removal casein of emulsion, also clear liquid can be removed macromolecular substances with the micropore ultrafiltration, get the high product of nutritional labeling.This product can be used for substituting calf serum as the nutrition adding ingredient in the cell culture fluid in the cell cultivation.
By multiple bioactive substance content height such as the product immunoglobulin (Ig) of the inventive method preparation, lysozymes, the casein content pettiness, helping infant and the elderly absorbs, infantile diarrhea, senile chronic bronchitis there are obvious prevention effect, also can be used as the nutritional labeling in the cell culture fluid.
Claims (4)
1, the preparation method of cow foremilk bio-active products, its technological process is that former breast is checked and accepted, is three layers with centrifuge with 3000-4000 rev/min of rotating speed centrifugation, remove upper strata fat deposit and bottom casein particle layer, emulsion in the middle of taking out, it is characterized in that in emulsion, adding rennin or pepsin or hydrochloric acid, act on half an hour to the visible grumeleuse of naked eyes occurring, be two-layer with centrifuge with 3000-4000 rev/min of rotating speed centrifugation again, remove the casein of precipitation, get supernatant and sterilized 30 minutes, be packaged as oral liquid or make the freeze drying product with cobalt-60 radiation sterilization or under 62 ℃ of temperature.
2, method according to claim 1, it is characterized in that the available ammonium sulfate method in supernatant sterilization back measure its immunoglobulin content (>30mg/ml), measure lysozyme content (1 μ g/ml), the eligible sterilization with the reference culture method.
3, method according to claim 1, after it is characterized in that casein is removed in the emulsion centrifugation, can be to the saturated ammonium sulfate that wherein adds clear liquid amount 30-50%, act on 30 minutes, the centrifuging and taking precipitation adds a small amount of dissolved in distilled water in precipitation, use the normal saline dialysis deionization again, must concentrate the immunoglobulin (Ig) highly finished product, again sterilization.
4, method according to claim 1, it is characterized in that casein is removed in the emulsion centrifugation after, macromolecular substances is removed in available micropore ultrafiltration, product.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 95100461 CN1112394A (en) | 1995-02-27 | 1995-02-27 | Preparation method for cow foremilk bio-active products |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 95100461 CN1112394A (en) | 1995-02-27 | 1995-02-27 | Preparation method for cow foremilk bio-active products |
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CN1112394A true CN1112394A (en) | 1995-11-29 |
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CN 95100461 Pending CN1112394A (en) | 1995-02-27 | 1995-02-27 | Preparation method for cow foremilk bio-active products |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1088332C (en) * | 1998-12-29 | 2002-07-31 | 黄河 | Extracting method for animal foremilk |
FR2865937A1 (en) * | 2004-02-11 | 2005-08-12 | Paul Vidal | Mammal colostoserum production procedure, especially for feeding to newborn calves, uses colostrum from healthy animal coagulated with rennet to obtain liquid |
CN100415104C (en) * | 2005-06-10 | 2008-09-03 | 黑龙江省完达山乳业股份有限公司 | A quark and preparation method thereof |
CN102429032A (en) * | 2011-11-14 | 2012-05-02 | 江苏星驰生物科技有限公司 | Cow fore milk formula powder for postoperative rehabilitation |
CN106359617A (en) * | 2016-08-30 | 2017-02-01 | 江苏天美健大自然生物工程有限公司 | Method for preparing bovine coloctrum products |
CN110981951A (en) * | 2019-12-26 | 2020-04-10 | 吉林大学 | Preparation method for enriching immunoglobulin and lactoferrin in colostrum |
RU2727504C1 (en) * | 2020-04-17 | 2020-07-22 | Общество с ограниченной ответственностью "Победа-1" (ООО "Победа-1") | Method for two-step membrane production of a hypoallergenic product based on immunoglobulin enriched lactoferrin for preventive dietary nutrition |
KR20210090560A (en) * | 2020-01-09 | 2021-07-20 | 세종대학교산학협력단 | Fetal Bovine Serum substitutes for cell culture |
-
1995
- 1995-02-27 CN CN 95100461 patent/CN1112394A/en active Pending
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1088332C (en) * | 1998-12-29 | 2002-07-31 | 黄河 | Extracting method for animal foremilk |
FR2865937A1 (en) * | 2004-02-11 | 2005-08-12 | Paul Vidal | Mammal colostoserum production procedure, especially for feeding to newborn calves, uses colostrum from healthy animal coagulated with rennet to obtain liquid |
CN100415104C (en) * | 2005-06-10 | 2008-09-03 | 黑龙江省完达山乳业股份有限公司 | A quark and preparation method thereof |
CN102429032A (en) * | 2011-11-14 | 2012-05-02 | 江苏星驰生物科技有限公司 | Cow fore milk formula powder for postoperative rehabilitation |
CN106359617A (en) * | 2016-08-30 | 2017-02-01 | 江苏天美健大自然生物工程有限公司 | Method for preparing bovine coloctrum products |
CN110981951A (en) * | 2019-12-26 | 2020-04-10 | 吉林大学 | Preparation method for enriching immunoglobulin and lactoferrin in colostrum |
CN110981951B (en) * | 2019-12-26 | 2023-03-28 | 吉林大学 | Preparation method for enriching immunoglobulin and lactoferrin in colostrum |
KR20210090560A (en) * | 2020-01-09 | 2021-07-20 | 세종대학교산학협력단 | Fetal Bovine Serum substitutes for cell culture |
KR102576778B1 (en) * | 2020-01-09 | 2023-09-12 | 세종대학교산학협력단 | Fetal Bovine Serum substitutes for cell culture |
RU2727504C1 (en) * | 2020-04-17 | 2020-07-22 | Общество с ограниченной ответственностью "Победа-1" (ООО "Победа-1") | Method for two-step membrane production of a hypoallergenic product based on immunoglobulin enriched lactoferrin for preventive dietary nutrition |
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