CN111235639B - Phage antibody elution device - Google Patents
Phage antibody elution device Download PDFInfo
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- CN111235639B CN111235639B CN202010152629.3A CN202010152629A CN111235639B CN 111235639 B CN111235639 B CN 111235639B CN 202010152629 A CN202010152629 A CN 202010152629A CN 111235639 B CN111235639 B CN 111235639B
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/02—Integrated apparatus specially adapted for creating libraries, screening libraries and for identifying library members
Abstract
The invention discloses a phage antibody elution device which comprises a frame body, wherein a water tank is arranged in the frame body, and two first cross beams are symmetrically arranged on two sides of the top of the frame body. According to the invention, through the arrangement of the upright columns and the guide of the fourth slide rail, the overall structural strength of the rack body is increased, meanwhile, the lifting of the cover plate is more stable, the addition of ice water in the water tank is facilitated, the technical problem that the addition of ice water is inconvenient as the cover plate is fixed on the water tank in the prior art is solved, the phage antibody is eluted by an ultrasonic method, the operation is simple, and the time is saved; no chemical reagent is involved in the elution process; can be used for multi-tube phage antibody elution at the same time; the method is not influenced by chemical reagents, and compared with the existing elution method, the method can greatly shorten the elution time and improve the elution efficiency.
Description
Technical Field
The invention relates to the technical field of phage antibody preparation, in particular to a phage antibody elution device.
Background
The phage antibody library technology is a breakthrough development of antibody library technology, which detects soluble expression products of bacterial clones in screening, but fuses Fab or scFV expressed on the surface of phage particles after phage vectors transform bacteria. Referred to as phage antibodies. Through multiple rounds of antigen adsorption-elution-amplification, the desired antibody clones are finally screened, thereby greatly simplifying the screening process. The screened phage antibody clone can obtain soluble Fab or scFV by using human amber mutant inserted and recipient bacterium which can not read amber mutant.
Patent document (CN 201620005596.9) discloses a phage antibody elution device, which uses an ultrasonic method to elute phage antibodies, and has simple operation and time saving; no chemical reagent is involved in the elution process; can be used for multi-tube phage antibody elution at the same time; the method is not influenced by chemical reagents, and compared with the existing elution method, the method can greatly shorten the elution time and improve the elution efficiency. However, the cover plate of the elution device is fixed on the water tank in the elution process, so that the addition of ice water is very inconvenient, and meanwhile, the elution device needs to be manually inserted when the immune tube and the ultrasonic amplitude transformer are fixed, so that the technical problem of low insertion efficiency is caused.
Disclosure of Invention
The invention aims to provide a phage antibody elution device, which solves the following technical problems: (1) the phage antibody is eluted by an ultrasonic method, the operation is simple, the time is saved, and no chemical reagent is involved in the elution process; can be used for multi-tube phage antibody elution at the same time; the method is not influenced by chemical reagents, compared with the existing elution method, the method can greatly shorten the elution time and improve the elution efficiency, the right-angle block is upwards pushed by a piston rod of the second air cylinder, the right-angle block drives the clamping plate to upwards move, the clamping plate drives the clamped cover plate to ascend through the clamping groove, then ice water is added into the inner cavity of the water tank, the second air cylinder drives the cover plate to descend after the ice water is added, and then the cover plate covers the water tank; (2) the immune tube and the ultrasonic amplitude transformer are effectively prevented from slipping in the transmission process in the guide cylinder by the arrangement of the three transmission belts matched with the supporting wheel, the immune tube and the ultrasonic amplitude transformer are effectively clamped in the transmission process in the guide cylinder, and the first air cylinder, the sliding block, the first rotating rod and the second rotating rod are effectively prevented from slipping in the downward transmission process, the distance between the three transmission belts can be adjusted, so that the transmission of the immunization tube and the ultrasonic amplitude transformer with different diameters is met, and the technical problem that the transmission of the immunization tube and the ultrasonic amplitude transformer is difficult to meet due to the fact that the immunization tube and the ultrasonic amplitude transformer have different diameters in the prior art is solved; (3) the first screw rod is driven to rotate through the first motor output shaft, the first screw rod is matched with the nut to drive the connecting block to move, the connecting block drives the connecting plate to slide on the first slide rail, the second motor output shaft drives the second screw rod to rotate, the second screw rod is matched with the nut to drive the right-angle plate to slide along the second slide rail, the third motor output shaft drives the third screw rod to rotate, the third screw rod is matched with the nut to drive the lifting plate to slide downwards along the third slide rail, the guide cylinder is further adjusted to be above the first through hole and the second through hole in the three-axis direction, the immune tube and the ultrasonic amplitude transformer are inserted into the first through hole and the second through hole through the transmission belt, through the arrangement, the guide cylinder can be adjusted in multiple positions on the cover plate, the immune tube and the ultrasonic amplitude transformer can be inserted into the first through hole and the second through hole on the cover plate, an operator does not need to, the technical problem that in the prior art, an immunization tube and an ultrasonic amplitude transformer need to be manually inserted into a water tank by an operator, and the insertion efficiency is low is solved.
The purpose of the invention can be realized by the following technical scheme:
a phage antibody elution device comprises a frame body, wherein a water tank is arranged in the frame body, two first cross beams are symmetrically arranged on two sides of the top of the frame body, a fixing plate is arranged on one side of each first cross beam, a first motor is arranged on each fixing plate, an output shaft of each first motor penetrates through each fixing plate, a first lead screw is arranged at the end of each first lead screw, the outer peripheral surface of each first lead screw is connected with a connecting block through a nut, and a connecting plate is arranged at the top of each connecting block;
the two connecting plates are respectively fixed on two sides of the bottom of the second cross beam, one side of the top of the second cross beam is provided with a second motor through a motor fixing seat, the end part of an output shaft of the second motor is provided with a second lead screw, the peripheral surface of the second lead screw is connected with a right-angle plate through a nut, the right-angle plate is connected with a second slide rail through a slide block in a sliding way, the right-angle plate is provided with a third motor, the end part of an output shaft of the third motor is provided with a third lead screw, the peripheral surface of the third lead screw is connected with a lifting plate through a nut, the lifting plate is provided with a guide cylinder, the top and the bottom of the guide cylinder are symmetrically provided with two guide ports, the inner wall of the guide cylinder is provided with three strip-shaped plates with equal radians, the strip-shaped plates are provided with slide holes, two first rotating rods are rotatably arranged on two sides of the sliding piece, one side, far away from the sliding piece, of each first rotating rod is rotatably connected with two side plates, four second rotating rods are rotatably arranged on two sides of the strip-shaped plate, one ends, far away from the strip-shaped plate, of the four second rotating rods are respectively rotatably connected with the two side plates, two driving wheels are arranged between the two side plates, and the two driving wheels are in transmission connection with a transmission belt;
the support body bilateral symmetry is provided with two second cylinders, second cylinder bilateral symmetry is provided with two stands, the stand is fixed in on the support body, second cylinder piston rod is connected with the right angle piece, the right angle piece is fixed in cardboard one side, the cardboard is kept away from right angle piece one side and is seted up the draw-in groove, and two draw-in grooves joint respectively in apron both sides, the apron sets up in the basin top, a plurality of first through-holes have been seted up on the apron, the second through-hole has still been seted up on the apron.
Furthermore, the lower surface of the connecting plate is connected with a first slide rail in a sliding mode through a slide block, the first slide rail is fixed to the top of the first cross beam, the two sides of the first lead screw are connected with a first rod seat in a rotating mode, and the first rod seat is fixed to the first cross beam.
Furthermore, both ends of the second screw rod are rotatably connected with second rod seats, and the second rod seats are fixed at the top of the second cross beam.
Furthermore, the number of the second slide rails is two, and the two second slide rails are fixed on the side surface of the second cross beam.
Furthermore, the lifting plate is connected with third slide rails through a slide block in a sliding manner, the number of the third slide rails is two, the two third slide rails are fixed on the right-angle plate, and the two third slide rails are respectively arranged on two sides of the third screw rod.
Furthermore, the transmission band sets up between two curb plates, and one of them drive wheel is connected the output shaft of fourth motor.
Furthermore, the clamping plate is connected with a fourth slide rail in a sliding mode through a sliding block, and the fourth slide rail is installed on the stand column.
Furthermore, the inner diameter of the second through hole is larger than that of the first through holes, the number of the first through holes is nine, and the nine first through holes are distributed on the cover plate in a rectangular array.
Further, two still be provided with a plurality of supporting wheels between the curb plate, two liang equidistance settings of a plurality of supporting wheels, a plurality of supporting wheels all contact the transmission band, still are provided with the support bar between two curb plates, the support bar contacts the transmission band.
Further, the elution device works as follows:
the method comprises the following steps: a piston rod of the second air cylinder pushes the right-angle block upwards, the right-angle block drives the clamping plate to move upwards, the clamping plate drives the clamped cover plate to ascend through the clamping groove, ice water is added into the inner cavity of the water tank, and the second air cylinder drives the cover plate to descend after the ice water is added, so that the cover plate covers the water tank;
step two: the immune tube is placed from a guide opening at the top of the guide cylinder, a piston rod of a first cylinder drives a sliding sheet to slide on a sliding hole, a first rotating rod on the sliding sheet is matched with a second rotating rod on a strip-shaped plate to rotate, so that three transmission belts in the guide cylinder are driven to clamp the immune tube, an output shaft of a fourth motor drives one of driving wheels to rotate, the driving wheels are matched with the transmission belts to downwards transmit the immune tube, and the supporting wheels ensure the clamping of the immune tube in the transmission process through the transmission belts;
step three: the first motor output shaft drives the first screw rod to rotate while the immune tube is conveyed downwards, the first screw rod is matched with the nut to drive the connecting block to move, the connecting block drives the connecting plate to slide on the first slide rail, the second motor output shaft drives the second screw rod to rotate, the second screw rod is matched with the nut to drive the right-angle plate to slide along the second slide rail, the third motor output shaft drives the third screw rod to rotate, the third screw rod is matched with the nut to drive the lifting plate to slide downwards along the third slide rail, the guide cylinder is adjusted to be above the first through hole in the three-axis direction, the immune tube is inserted into the first through hole by the transmission belt and is immersed into the ice water in the water tank, then the three transmission belts cancel the clamping of the immune tube, the guide cylinder returns to the initial position, the ultrasonic amplitude transformer of the ultrasonic crusher is inserted into the guide port at the top of the guide cylinder, the distance between the three transmission belts is adjusted, the ultrasonic amplitude, the transmission belt inserts the ultrasonic horn into the ice water in the water bath.
The invention has the beneficial effects that:
(1) according to the phage antibody elution device, the phage antibody is eluted by an ultrasonic method, the operation is simple, the time is saved, and no chemical reagent is involved in the elution process; can be used for multi-tube phage antibody elution at the same time; the method is not influenced by chemical reagents, compared with the existing elution method, the method can greatly shorten the elution time and improve the elution efficiency, the right-angle block is upwards pushed by a piston rod of the second air cylinder, the right-angle block drives the clamping plate to upwards move, the clamping plate drives the clamped cover plate to ascend through the clamping groove, then ice water is added into the inner cavity of the water tank, the second air cylinder drives the cover plate to descend after the ice water is added, and then the cover plate covers the water tank;
(2) the immune tube and the ultrasonic amplitude transformer are put into the guide cylinder from a guide opening at the top of the guide cylinder, a first cylinder piston rod drives a slip sheet to slide on a slide hole, a first rotating rod on the slip sheet is matched with a second rotating rod on a strip-shaped plate to rotate, and then three transmission belts in the guide cylinder are driven to clamp the immune tube and the ultrasonic amplitude transformer, a fourth motor output shaft drives one of the driving wheels to rotate, the driving wheel is matched with the transmission belts to transmit the immune tube and the ultrasonic amplitude transformer downwards, the supporting wheels ensure the clamping of the immune tube and the ultrasonic amplitude transformer in the transmission process through the transmission belts, the immune tube and the ultrasonic amplitude transformer can be effectively clamped in the transmission process in the guide cylinder through the matching of the three transmission belts and the arrangement of the supporting wheels, the slipping condition of the immune tube and the ultrasonic amplitude transformer in the downward transmission process is effectively prevented, and the arrangement of the first cylinder, the sliding block, the first rotating rod and the, the distance between the three transmission belts can be adjusted, so that the transmission of the immune tubes and the ultrasonic amplitude transformer with different diameters is met, and the practicability is strong;
(3) the first motor output shaft drives the first screw rod to rotate, the first screw rod drives the connecting block to move by matching with the nut, the connecting block drives the connecting plate to slide on the first slide rail, the second motor output shaft drives the second screw rod to rotate, the second screw rod drives the right-angle plate to slide along the second slide rail by matching with the nut, the third motor output shaft drives the third screw rod to rotate, the third screw rod drives the lifting plate to slide downwards along the third slide rail by matching with the nut, and further the guide cylinder is adjusted to be above the first through hole and the second through hole in the three-axis direction, the immune tube and the ultrasonic amplitude transformer are inserted into the first through hole and the second through hole on the cover plate by the transmission belt, through the arrangement, the guide cylinder can be adjusted in multiple positions on the cover plate, the immune tube and the ultrasonic amplitude transformer can be inserted into the first through hole and the second through hole on the cover plate, and an operator does not, the efficiency of inserting the immune tube and the ultrasonic amplitude transformer is effectively improved.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view showing the structure of a phage antibody elution apparatus according to the present invention;
FIG. 2 is a schematic structural view of a first cross member of the present invention;
FIG. 3 is a schematic structural view of a second cross member of the present invention;
FIG. 4 is an internal structural view of the guide cylinder of the present invention;
FIG. 5 is a schematic view of a structure of a transfer belt according to the present invention;
FIG. 6 is a schematic view of the mounting of the card of the present invention;
FIG. 7 is a rear elevational view of the card of the present invention;
fig. 8 is a schematic structural view of the cover plate of the present invention.
In the figure: 1. a frame body; 2. a first cross member; 3. a second cross member; 4. a water tank; 5. a column; 6. a fixing plate; 7. a first slide rail; 8. a first motor; 9. a first lead screw; 91. a first lever seat; 10. a connecting plate; 101. connecting blocks; 11. a second motor; 12. a second lead screw; 121. a second rod seat; 13. a second slide rail; 14. a right-angle plate; 15. a third motor; 16. a third screw rod; 17. a third slide rail; 18. a lifting plate; 19. a guide cylinder; 191. a guide port; 20. a strip plate; 201. a slide hole; 21. a first cylinder; 22. sliding blades; 23. a first rotating lever; 24. a second rotating lever; 25. a fourth motor; 26. a side plate; 261. a support wheel; 262. a supporting strip; 27. a drive wheel; 28. a conveyor belt; 29. a second cylinder; 30. a right-angle block; 31. clamping a plate; 32. a card slot; 33. a cover plate; 331. a first through hole; 332. a second through hole; 34. and a fourth slide rail.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, the invention relates to a phage antibody elution device, which comprises a frame body 1, wherein a water tank 4 is arranged in the frame body 1, two first beams 2 are symmetrically arranged on two sides of the top of the frame body 1, a fixing plate 6 is arranged on one side of each first beam 2, a first motor 8 is arranged on each fixing plate 6, an output shaft of each first motor 8 penetrates through each fixing plate 6, a first screw rod 9 is arranged at the end of each first motor 8, the peripheral surface of each first screw rod 9 is connected with a connecting block 101 through a nut, and a connecting plate 10 is arranged at the;
two connecting plates 10 are respectively fixed on two sides of the bottom of a second beam 3, one side of the top of the second beam 3 is provided with a second motor 11 through a motor fixing seat, the end part of an output shaft of the second motor 11 is provided with a second lead screw 12, the peripheral surface of the second lead screw 12 is connected with a right-angle plate 14 through a nut, the right-angle plate 14 is connected with a second slide rail 13 through a slide block in a sliding way, the right-angle plate 14 is provided with a third motor 15, the end part of the output shaft of the third motor 15 is provided with a third lead screw 16, the peripheral surface of the third lead screw 16 is connected with a lifting plate 18 through a nut, the lifting plate 18 is provided with a guide barrel 19, the top and the bottom of the guide barrel 19 are symmetrically provided with two guide ports 191, the inner wall of the guide barrel 19 is provided with three strip-shaped plates 20 with equal radians, the strip-shaped plates 20, two first rotating rods 23 are rotatably arranged on two sides of the sliding piece 22, one side, far away from the sliding piece 22, of each first rotating rod 23 is rotatably connected with two side plates 26, four second rotating rods 24 are rotatably arranged on two sides of the strip-shaped plate 20, one ends, far away from the strip-shaped plate 20, of the four second rotating rods 24 are respectively rotatably connected with the two side plates 26, two driving wheels 27 are arranged between the two side plates 26, and the two driving wheels 27 are in transmission connection with a transmission belt 28;
1 bilateral symmetry of support body is provided with two second cylinders 29, 29 bilateral symmetry of second cylinder is provided with two stands 5, stand 5 is fixed in on the support body 1, 29 piston rods of second cylinder are connected with right angle block 30, right angle block 30 is fixed in cardboard 31 one side, cardboard 31 is kept away from right angle block 30 one side and has been seted up draw-in groove 32, two draw-in grooves 32 joint respectively in apron 33 both sides, apron 33 sets up in basin 4 top, a plurality of first through-holes 331 have been seted up on the apron 33, second through-hole 332 has still been seted up on the apron 33.
Specifically, the lower surface of the connecting plate 10 is connected with a first slide rail 7 in a sliding manner through a slide block, the first slide rail 7 is fixed at the top of the first cross beam 2, the two sides of the first lead screw 9 are both rotatably connected with a first rod seat 91, and the first rod seat 91 is fixed on the first cross beam 2. Both ends of the second screw rod 12 are rotatably connected with a second rod seat 121, and the second rod seat 121 is fixed on the top of the second beam 3. The number of the second slide rails 13 is two, and the two second slide rails 13 are fixed on the side surface of the second beam 3. The lifting plate 18 is connected with the third slide rails 17 in a sliding mode through the slide blocks, the number of the third slide rails 17 is two, the two third slide rails 17 are fixed on the right-angle plate 14, and the two third slide rails 17 are arranged on two sides of the third screw rod 16 respectively. A transmission belt 28 is arranged between the two side plates 26, wherein one driving wheel 27 is connected to the output shaft of the fourth motor 25. The clamping plate 31 is connected with a fourth slide rail 34 in a sliding manner through a sliding block, and the fourth slide rail 34 is installed on the upright post 5. The inner diameter of the second through holes 332 is larger than that of the first through holes 331, the number of the first through holes 331 is nine, and the nine first through holes 331 are distributed on the cover plate 33 in a rectangular array. Still be provided with a plurality of supporting wheel 261 between two curb plates 26, the setting of two liang of equidistance of a plurality of supporting wheel 261, a plurality of supporting wheel 261 all contact transmission band 28, still is provided with support bar 262 between two curb plates 26, and support bar 262 contact transmission band 28.
Referring to FIGS. 1 to 8, the operation of a phage antibody elution apparatus of this embodiment is as follows:
the method comprises the following steps: the piston rod of the second cylinder 29 pushes the right-angle block 30 upwards, the right-angle block 30 drives the clamping plate 31 to move upwards, the clamping plate 31 drives the clamped cover plate 33 to ascend through the clamping groove 32, then ice water is added into the inner cavity of the water tank 4, the second cylinder 29 drives the cover plate 33 to descend after the ice water is added, and then the cover plate 33 covers the water tank 4;
step two: putting the immune tube into the guide cylinder 19 from the top guide port 191, driving the sliding sheet 22 to slide on the sliding hole 201 by the piston rod of the first air cylinder 21, further driving the first rotating rod 23 on the sliding sheet 22 to rotate in cooperation with the second rotating rod 24 on the strip-shaped plate 20, further driving the three transmission belts 28 in the guide cylinder 19 to clamp the immune tube, driving one of the driving wheels 27 to rotate by the output shaft of the fourth motor 25, driving the driving wheel 27 to downwards transmit the immune tube in cooperation with the transmission belts 28, and ensuring the clamping of the immune tube in the transmission process by the supporting wheel 261 through the transmission belts 28;
step three: when the immune tube is conveyed downwards, the output shaft of the first motor 8 drives the first screw rod 9 to rotate, the first screw rod 9 is matched with the nut to drive the connecting block 101 to move, the connecting block 101 drives the connecting plate 10 to slide on the first slide rail 7, the output shaft of the second motor 11 drives the second screw rod 12 to rotate, the second screw rod 12 is matched with the nut to drive the right-angle plate 14 to slide along the second slide rail 13, the output shaft of the third motor 15 drives the third screw rod 16 to rotate, the third screw rod 16 is matched with the nut to drive the lifting plate 18 to slide downwards along the third slide rail 17, the guide cylinder 19 is adjusted to be positioned above the first through hole 331 in the three-axis direction, the immune tube is inserted into the first through hole 331 by the conveying belts 28 and is immersed into ice water in the water tank 4, then the three conveying belts 28 cancel clamping of the immune tube, the guide cylinder 19 returns to the initial position, the ultrasonic amplitude-changing rod of the ultrasonic crusher is inserted into, the ultrasonic horn is clamped, then the guide cylinder 19 is adjusted to be above the second through hole 332 through the three shafts, and the transmission belt 28 inserts the ultrasonic horn into the ice water in the water tank 4.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.
Claims (10)
1. The phage antibody elution device is characterized by comprising a frame body (1), wherein a water tank (4) is arranged in the frame body (1), two first cross beams (2) are symmetrically arranged on two sides of the top of the frame body (1), a fixing plate (6) is installed on one side of each first cross beam (2), a first motor (8) is installed on each fixing plate (6), an output shaft of each first motor (8) penetrates through each fixing plate (6), a first lead screw (9) is installed at the end of each first cross beam (2), the outer peripheral surface of each first lead screw (9) is connected with a connecting block (101) through a nut, and a connecting plate (10) is installed at the top of each connecting block (;
two connecting plates (10) are respectively fixed on two sides of the bottom of a second cross beam (3), one side of the top of the second cross beam (3) is provided with a second motor (11) through a motor fixing seat, the end part of an output shaft of the second motor (11) is provided with a second lead screw (12), the peripheral surface of the second lead screw (12) is connected with a right-angle plate (14) through a nut, the right-angle plate (14) is connected with a second slide rail (13) through a slide block in a sliding way, the right-angle plate (14) is provided with a third motor (15), the end part of the output shaft of the third motor (15) is provided with a third lead screw (16), the peripheral surface of the third lead screw (16) is connected with a lifting plate (18) through a nut, the lifting plate (18) is provided with a guide barrel (19), the top and the bottom of the guide barrel (19) are symmetrically provided with two guide ports (191), the, the sliding hole (201) is formed in the strip-shaped plate (20), a sliding piece (22) is arranged on the sliding hole (201) in a sliding mode, a first air cylinder (21) is installed on the strip-shaped plate (20), a piston of the first air cylinder (21) is connected with the sliding piece (22), two first rotating rods (23) are arranged on two sides of the sliding piece (22) in a rotating mode, one sides, far away from the sliding piece (22), of the two first rotating rods (23) are connected with two side plates (26) in a rotating mode, four second rotating rods (24) are arranged on two sides of the strip-shaped plate (20) in a rotating mode, one ends, far away from the strip-shaped plate (20), of the four second rotating rods (24) are respectively connected with the two side plates (26) in a rotating mode, two driving;
support body (1) bilateral symmetry is provided with two second cylinders (29), second cylinder (29) bilateral symmetry is provided with two stand (5), stand (5) are fixed in on support body (1), second cylinder (29) piston rod is connected with right angle piece (30), right angle piece (30) are fixed in cardboard (31) one side, cardboard (31) are kept away from right angle piece (30) one side and have been seted up draw-in groove (32), and two draw-in grooves (32) joint respectively in apron (33) both sides, apron (33) set up in basin (4) top, a plurality of first through-holes (331) have been seted up on apron (33), second through-hole (332) have still been seted up on apron (33).
2. The phage antibody elution device according to claim 1, wherein the lower surface of the connecting plate (10) is slidably connected with a first sliding rail (7) through a sliding block, the first sliding rail (7) is fixed on the top of the first beam (2), both sides of the first screw rod (9) are rotatably connected with a first rod seat (91), and the first rod seat (91) is fixed on the first beam (2).
3. The phage antibody elution device of claim 1, wherein the second screw (12) is rotatably connected with a second rod seat (121) at both ends, and the second rod seat (121) is fixed on the top of the second beam (3).
4. The phage antibody elution apparatus of claim 1, wherein the number of the second slide rails (13) is two, and two second slide rails (13) are fixed on the side of the second beam (3).
5. The phage antibody elution device according to claim 1, wherein the lifting plate (18) is slidably connected with a third slide rail (17) through a slider, the number of the third slide rails (17) is two, two third slide rails (17) are fixed on the right-angle plate (14), and the two third slide rails (17) are respectively arranged on two sides of the third screw rod (16).
6. A phage antibody elution apparatus according to claim 1, characterized in that the conveyor belt (28) is disposed between two side plates (26), and one of the driving wheels (27) is connected to the output shaft of the fourth motor (25).
7. A phage antibody elution apparatus according to claim 1, wherein the said clamping plate (31) is slidably connected to a fourth slide rail (34) through a sliding block, and the said fourth slide rail (34) is installed on the upright (5).
8. A phage antibody elution apparatus according to claim 1, characterized in that the inner diameter of said second through hole (332) is larger than the inner diameter of said first through hole (331), the number of said first through holes (331) is nine, and nine first through holes (331) are distributed on the cover plate (33) in a rectangular array.
9. The phage antibody elution device of claim 1, wherein a plurality of support wheels (261) are further disposed between the two side plates (26), the support wheels (261) are disposed at equal intervals in pairs, the support wheels (261) are in contact with the conveyor belt (28), and a support strip (262) is further disposed between the two side plates (26), and the support strip (262) is in contact with the conveyor belt (28).
10. The phage antibody elution apparatus of claim 1, wherein the elution apparatus operates as follows:
the method comprises the following steps: a piston rod of the second air cylinder (29) pushes the right-angle block (30) upwards, the right-angle block (30) drives the clamping plate (31) to move upwards, the clamping plate (31) drives the clamped cover plate (33) to ascend through the clamping groove (32), ice water is added into the inner cavity of the water tank (4), the second air cylinder (29) drives the cover plate (33) to descend after the ice water is added, and the cover plate (33) covers the water tank (4);
step two: putting the immune tube into a guide opening (191) in the top of a guide cylinder (19), driving a sliding sheet (22) to slide on a sliding hole (201) by a piston rod of a first air cylinder (21), matching a first rotating rod (23) on the sliding sheet (22) with a second rotating rod (24) on a strip-shaped plate (20) to rotate, driving three transmission belts (28) in the guide cylinder (19) to clamp the immune tube, and transmitting the immune tube downwards by the transmission belts (28);
step three: when the immune tube is conveyed downwards, an output shaft of a first motor (8) drives a first screw rod (9) to rotate, the first screw rod (9) is matched with a nut to drive a connecting block (101) to move, an output shaft of a second motor (11) drives a second screw rod (12) to rotate, the second screw rod (12) is matched with a nut to drive a right-angle plate (14) to move, an output shaft of a third motor (15) drives a third screw rod (16) to rotate, the third screw rod (16) is matched with a nut to drive a lifting plate (18) to move, a guide cylinder (19) is adjusted to be positioned above a first through hole (331) in the three-axis direction, the immune tube is inserted into the first through hole (331) by a transmission belt (28) and is immersed into ice water in a water tank (4), then the three transmission belts (28) cancel clamping of the immune tube, the guide cylinder (19) returns to the initial position, an ultrasonic amplitude-changing rod of an ultrasonic crusher is inserted into a guide, distance adjustment between three transmission bands (28) is carried out, and the centre gripping is carried out to ultrasonic amplitude transformer, and then guide section of thick bamboo (19) are adjusted to second through-hole (332) top through the triaxial, and ultrasonic amplitude transformer is inserted in the ice water of basin (4) with transmission band (28), begins ultrasonic elution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010152629.3A CN111235639B (en) | 2020-03-06 | 2020-03-06 | Phage antibody elution device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010152629.3A CN111235639B (en) | 2020-03-06 | 2020-03-06 | Phage antibody elution device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111235639A CN111235639A (en) | 2020-06-05 |
| CN111235639B true CN111235639B (en) | 2021-04-20 |
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