CN111235057B - Biological agent for treating polyacrylamide wastewater and preparation method and application thereof - Google Patents
Biological agent for treating polyacrylamide wastewater and preparation method and application thereof Download PDFInfo
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- CN111235057B CN111235057B CN202010077830.XA CN202010077830A CN111235057B CN 111235057 B CN111235057 B CN 111235057B CN 202010077830 A CN202010077830 A CN 202010077830A CN 111235057 B CN111235057 B CN 111235057B
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Abstract
The invention provides a biological agent for treating polyacrylamide wastewater and a preparation method and application thereof, relating to the technical field of sewage treatment; a biological agent for treating polyacrylamide wastewater comprises PAM-De-Yan-1# and PAM-De-Yan-3# which are mixed in a ratio of 1: 1-1: 3. The biological agent can improve the degradation rate of PAM in the existing PAM biological treatment unit and the removal rate of total organic carbon and chemical oxygen demand. After biological strengthening, the PAM degradation rate is 75-80% when the PAM concentration is 100mg/L, the TOC removal rate is 55-60%, and the COD removal rate is 65-75%. When the concentration of PAM is 400mg/L, the degradation rate of PAM is 60-75%, the removal rate of TOC is 45-55%, and the removal rate of COD is 65-75%.
Description
Technical Field
The invention relates to the technical field of sewage treatment, and particularly relates to a biological agent for treating polyacrylamide wastewater, and a preparation method and application thereof.
Background
The waste water containing polyacrylamide mainly comes from the fields of oil field flooding and mining waste water, textile, water treatment and the like, and the foreign main application fields are water treatment, papermaking, mines, metallurgy and the like. At present, the usage amount of polyacrylamide in the fields of water treatment and papermaking is fastest. The waste water containing polyacrylamide has the characteristics of high viscosity, high COD, poor biodegradability and the like, and at present, a plurality of different treatment methods mainly comprise a physical and chemical method and a biological method aiming at the waste water at home and abroad. The utilization of the physiological property of microorganisms to treat refractory substances has become one of the efficient and thorough means for treating pollutants in the environmental field.
Because the produced water of the ASP flooding has the characteristics of high polymer content, high viscosity, high pH value, poor biodegradability and the like, the research on an effective biological treatment method of the wastewater of the ASP flooding is less at present. The main difficulty of the ternary combination flooding wastewater biological treatment is that the wastewater quality is complex, the polymer molecular weight is difficult to biodegrade, and the biological enhancement of a single strain is difficult to achieve the expected treatment effect. At present, most of the research of scholars at home and abroad is limited in the separation and culture of degradation bacteria, and the treatment effect of the polyacrylamide-containing wastewater is hoped to be improved through biological enhancement. However, the screened degrading bacteria are difficult to maintain a long-term effective treatment effect in an actual treatment system, so that an economic and long-term effective composite biological enhancement process for treating different pollutant components of the ASP flooding produced water is urgently needed.
Disclosure of Invention
The first purpose of the invention is to efficiently treat waste water containing polyacrylamide, and the invention provides a biological agent for treating the waste water containing polyacrylamide;
the second purpose of the invention is to provide a preparation method of a biological agent for treating polyacrylamide wastewater;
the third purpose of the invention is to provide the application of the biological agent for treating the polyacrylamide wastewater;
the invention is realized by the following technical scheme:
a biological agent for treating polyacrylamide wastewater comprises PAM-De-Yan-1# and PAM-De-Yan-3#, wherein the PAM-De-Yan 1# is Bacillus (Bacillus sp.), which has been preserved in the China general microbiological culture Collection center with the preservation number of No.16075 and the preservation date of 2018, 7 and 9; PAM-De-Yan 3# is Acinetobacter winogradskyi (Acinetobacter venenianus), and is preserved in the general microbiological center of China Committee for culture Collection of microorganisms with the preservation number of 16076 and the preservation date of 2018, 7 months and 9 days.
A preparation method of a biological agent for treating polyacrylamide wastewater comprises the following steps:
respectively inoculating PAM-De-Yan 1# and PAM-De-Yan 3# into an LB liquid culture medium, respectively activating at 30-37 ℃ for 24h, then carrying out centrifugal separation, removing supernatant, respectively diluting the centrifuged precipitate to 1OD by using sterile water, and obtaining activated bacterial liquid of PAM-De-Yan 1# and activated bacterial liquid of PAM-De-Yan 3 #;
step two, respectively carrying out fermentation culture on the two strains by adopting a fermentation tank; firstly, strictly sterilizing a fermentation tank, culturing PAM-De-Yan 1# activated bacterial liquid and PAM-De-Yan 3# activated bacterial liquid by adopting an LB liquid culture medium, controlling the dissolved oxygen to be 30-50%, controlling the pH to be 7.0-7.2, controlling the temperature to be 28-30 ℃, and harvesting the obtained thallus by adopting a centrifugation mode after culturing for 36-48 hours;
step three, drying the two obtained thalli at 40-50 ℃ to obtain dry powder; and mixing the PAM-De-Yan 1# and the dry powder of the PAM-De-Yan 3# to obtain the biological agent for treating the polyacrylamide wastewater.
The application of the biological agent for treating the polyacrylamide wastewater comprises the following steps:
The invention has the beneficial effects that: the invention utilizes the microorganism as the biological degradation agent of the polyacrylamide, and achieves the effects of quick start, high-efficiency treatment and operation cost reduction by adding the exogenous biological agent under the existing wastewater treatment device. Can improve the degradation rate of PAM, the removal rate of Total Organic Carbon (TOC) and Chemical Oxygen Demand (COD) in the existing PAM biological treatment unit. Has effect on PAM concentration of 100mg/L to 400 mg/L. After biological strengthening, the PAM degradation rate is 75-80% when the PAM concentration is 100mg/L, the TOC removal rate is 55-60%, and the COD removal rate is 65-75%. When the concentration of PAM is 400mg/L, the degradation rate of PAM is 60-75%, the removal rate of TOC is 45-55%, and the removal rate of COD is 65-75%.
Drawings
FIG. 1: growth curve diagrams of the bacteria PAM-De-Yan-1#, PAM-De-Yan-3#, and the mixed biological agent PAM-De-Yan-1-3# in a liquid culture medium with the PAM concentration of 300 mg/L;
FIG. 2: after the biological agent PAM-De-Yan-1-3# is strengthened, a graph of the removal effect of the biological contact oxidation reactor COR on the viscosity and COD of PAM (with the molecular weight of 2200 ten thousand) with different concentrations is shown;
FIG. 3: after the biological agent PAM-De-Yan-1-3# is strengthened, a removing effect graph of a biological contact oxidation reactor COR on 100mg/LPAM is shown;
FIG. 4 is a schematic view of: after the biological agent PAM-De-Yan-1-3# is strengthened, a graph of the effect of COR on the removal of Total Organic Carbon (TOC) of PAM wastewater with different concentrations is shown;
FIG. 5: and (3) after the biological agent PAM-De-Yan-1-3# is strengthened, a graph of the effect of COR on the removal of Total Nitrogen (TN) of PAM wastewater with different concentrations is shown.
Detailed Description
The technical solutions of the present invention are further described below with reference to fig. 1 to 5 and the detailed description, but not limited thereto, and all modifications or equivalent substitutions that do not depart from the spirit and scope of the technical solutions of the present invention should be covered by the protection scope of the present invention.
The invention obtains polyacrylamide degradation strains through screening, compounds different microorganism strains into biological microbial inoculum, and utilizes the biological microbial inoculum to strengthen the biodegradation of polyacrylamide-containing wastewater for treating the polyacrylamide-containing wastewater, such as oil field polymer flooding wastewater, wastewater generated in the polyacrylamide production process, and the like.
Detailed description of the invention
A biological agent for treating polyacrylamide wastewater comprises PAM-De-Yan-1# and PAM-De-Yan-3# with a thallus mixing ratio of 1: 1-1: 3, wherein the PAM-De-Yan 1# is Bacillus (Bacillus sp.) which is preserved in China general microbiological culture Collection center with the preservation address of No. 3 institute No.1 West Chen of the sunward area in Beijing, the preservation number is No.16075, and the preservation date is 7 months and 9 days in 2018.
PAM-De-Yan 3# is Acinetobacter vini (Acinetobacter venenianus) which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of Xilu No.1 of Beijing Kogyo, the facing-sunny area, the preservation number is No.16076, and the preservation date is 7 months and 9 days in 2018.
Detailed description of the invention
A preparation method of a biological agent for treating polyacrylamide wastewater comprises the following steps:
respectively inoculating PAM-De-Yan 1# and PAM-De-Yan 3# to an LB liquid culture medium, activating at 30-37 ℃ for 24h, centrifuging at 5000-8000 r/min for 5-10 min after activation, discarding supernatant, and respectively diluting the centrifuged precipitate to 1OD by using sterile water to obtain activated bacterial liquid of PAM-De-Yan 1# and activated bacterial liquid of PAM-De-Yan 3 #;
step two, respectively carrying out fermentation culture on the two strains by adopting a 5L fermentation tank; firstly, strictly sterilizing a fermentation tank, and culturing PAM-De-Yan 1# activated bacterial liquid and PAM-De-Yan 3# activated bacterial liquid by adopting an LB liquid culture medium, wherein the dissolved oxygen is controlled to be 30-50%, the pH is controlled to be 7.0-7.2, and the temperature is controlled to be 28-30 ℃; after culturing for 36-48 h, harvesting the obtained thalli in a centrifugal mode, wherein the centrifugal rotating speed is 16000g/min, and the centrifugal time is 10 min;
step three, drying the obtained thalli at 40-50 ℃ to obtain dry powder; mixing the dried thallus powder of PAM-De-Yan 1# and PAM-De-Yan 3# according to the mass ratio of 1: 1-1: 3 to obtain a biological agent PAM-De-Yan-1-3# for treating polyacrylamide wastewater; the biological agent is stored for a long time at 4 ℃.
Further, screening of amide-group strains in the high-efficiency degradation polyacrylamide: taking soil which is in long-term contact with polymer-containing produced water in Dongying Shengli oil field as a source for microorganism screening, firstly, taking a liquid culture medium taking PAM as a unique nitrogen source as a screening and enrichment culture medium I, weighing 5-10 g of soil, adding the soil into 100mL of sterile water, adding 1g of glass beads, shaking by a shaking table at 120-150 rpm for 1h, taking 1mL of supernatant, adding the supernatant into the culture medium I, and culturing for 5d at 30 ℃; then 1mL of the cultured bacterial solution is added into a new 100mL of culture medium I and cultured for 48h at 30 ℃. Adding agar with the mass percent of 1.5-2.0% into the culture medium I to prepare a solid plate, and obtaining the pure strain after 5-6 times of plate coating and streak separation. Comparing the degradation effects of different strains, determining the strain with the best PAM degradation effect, and identifying to obtain the strain named PAM-De-Yan 1 #. The strain can grow on LB solid culture medium inclined tube, and can be stored for more than 1 year at 4 deg.C after being packaged with sterile paraffin.
Further, screening of strains for efficiently degrading carbon-containing parts in polyacrylamide: a biological membrane on the surface of a filler in a biological contact oxidation reactor for treating polyacrylamide PAM wastewater in a long-term contact manner is used as a source for screening microorganisms, and a liquid culture medium taking sodium polyacrylate as a unique carbon source is used as a screening and enriching culture medium II (the sodium polyacrylate is a part of polyacrylamide after amide removal and is soluble in water). 5g of biological membrane in the biological contact oxidation reactor is scraped and weighed, the biological membrane is added into 100mL of sterile water, 1g of glass beads are added, and the shaking table with 120rpm shakes for 1 h. Adding 1mL of supernatant into the culture medium II, and culturing at 30 ℃ for 5 d; then 1mL of the cultured bacterial solution is added into a new 100mL of culture medium II and cultured for 48h at 30 ℃. Adding agar with the mass percent of 1.5-2.0% into the culture medium II to prepare a solid plate, and obtaining the pure strain after 5-6 times of plate coating and streak separation. Comparing TOC degradation effects of different strains, determining the strain with the best polyacrylic acid degradation effect, and identifying to obtain the strain named PAM-De-Yan 3 #. The strain can grow on an LB solid culture medium inclined tube, is packaged by sterile paraffin, and can be stored for more than 1 year at the temperature of 4 ℃.
Further, the composition of the medium I (calculated by 1L): 2mL of liquid paraffin, 0.3g of PAM, 0.2g of KH2PO4,0.1g CaCl2,0.1g MgCl2，1g NaCl，0.1g FeCl3，0.1g MgSO4And 1mL of trace element liquid (solution prepared by various trace elements), and adding deionized water to a constant volume of 1L. Wherein the formula of the trace element liquid is (counted by L): 1.5g N (CH)2COOH)3，3.0g MgSO4，1.0gNaCl，0.5g MnSO4，0.1g FeSO4，0.1g CaCl2，0.1gCoCl2，0.1gZnSO4，0.01g H3BO4，0.01g KAl(SO4)2，0.01g CuSO4，0.01g NaMoO4And adding deionized water to the solution to make the volume of the solution constant to 1L.
Further, the composition of the medium II (calculated as 1L): the components of the culture medium are as follows: 1g of sodium polyacrylate, 0.1g of NH4Cl，0.2g KH2PO4,0.1g CaCl2,0.1g MgCl2，1gNaCl，0.1g FeCl3，0.1g MgSO4And 1mL of trace element liquid (solution prepared by various trace elements), and adding deionized water to a constant volume of 1L. Wherein the formula of the trace element liquid is (counted by L): 1.5gN (CH)2COOH)3，3.0g MgSO4，1.0g NaCl，0.5g MnSO4，0.1g FeSO4，0.1g CaCl2，0.1gCoCl2，0.1gZnSO4，0.01g H3BO4，0.01g KAl(SO4)2，0.01g CuSO4，0.01g NaMoO4And adding deionized water to the solution to make the volume of the solution constant to 1L.
Further, the LB liquid medium comprises (1L) 10g of tryptone, 5g of yeast extract and 10g of NaCl, the pH value is adjusted to 7.0, and the LB solid medium is prepared by adding 15-20 g of agar to the LB liquid medium.
Detailed description of the invention
The application of the biological agent for treating the polyacrylamide wastewater comprises the following steps:
After the microbial inoculum is inoculated, water can be fed normally and continuously, and the removal effect of the reactor for 5d on PAM can be monitored continuously. If the ideal effect is not achieved, the inoculation can be carried out again.
Further, in the step 1, the volume of the COR reactor is 75L, aeration is carried out in the whole process, the dissolved oxygen of the reactor is kept at 3-4 mg/L, the operation period is 24h, wherein the adding amount of the filler in the COR reactor accounts for 40% of the volume of the whole reactor, and the filler is a disc (phi 150) -dendritic (60 cm in length) combined type hydroformylation fiber yarn polypropylene filler.
Claims (6)
1. A biological agent for treating polyacrylamide wastewater is characterized in that: consists of PAM-De-Yan-1# and PAM-De-Yan-3#, wherein, PAM-De-Yan 1# is bacillus (Bacillus: (Bacillus sp.) The culture medium has been preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.16075 and the preservation date of 2018, 7 months and 9 days; PAM-De-Yan 3# is Acinetobacter Veneti: (Acinetobacter venetianus)，Has been preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation number is CGMCC No.16076, and the preservation date is 7 months and 9 days in 2018.
2. The biological agent for treating polyacrylamide waste water as claimed in claim 1, wherein: the mass ratio of the dry thallus powder of the PAM-De-Yan-1# to the dry thallus powder of the PAM-De-Yan-3# is 1: 1-1: 3.
3. The preparation method of the biological agent for treating the polyacrylamide wastewater as claimed in claim 1 or 2, characterized by comprising the following steps:
respectively inoculating PAM-De-Yan 1# and PAM-De-Yan 3# into an LB liquid culture medium, respectively activating at 30-37 ℃ for 24h, then carrying out centrifugal separation, removing supernatant, respectively diluting the centrifuged precipitate to 1OD by using sterile water, and obtaining activated bacterial liquid of PAM-De-Yan 1# and activated bacterial liquid of PAM-De-Yan 3 #;
step two, respectively carrying out fermentation culture on the two strains by adopting a fermentation tank; firstly, strictly sterilizing a fermentation tank, culturing PAM-De-Yan 1# activated bacterial liquid and PAM-De-Yan 3# activated bacterial liquid by adopting an LB liquid culture medium, controlling the dissolved oxygen to be 30-50%, controlling the pH to be 7.0-7.2, controlling the temperature to be 28-30 ℃, and harvesting the obtained thallus by adopting a centrifugation mode after culturing for 36-48 hours;
step three, drying the two obtained thalli at 40-50 ℃ to obtain dry powder; and mixing the PAM-De-Yan 1# and the dry powder of the PAM-De-Yan 3# to obtain the biological agent for treating the polyacrylamide wastewater.
4. The application of the biological agent for treating the polyacrylamide wastewater as claimed in claim 1 or 2, which is characterized by comprising the following steps:
step 1, adding activated sludge into a biological contact oxidation reactor, wherein the sludge concentration is 4000mg/L, the influent water is domestic sewage, the PAM concentration is added into the domestic sewage to 80mg/L, and the COD is 300-400 mg/L; when the biological contact oxidation reactor operates for 24 hours every time, the concentration of PAM is adjusted up to 20mg/L until the concentration of PAM is increased to 200mg/L, and then the biological contact oxidation reactor continuously operates for 15-30 days to finish domestication;
step 2, treating the biological agent for the polyacrylamide wastewater according to the ratio of 20-50 g/m3Is added into a biological contact oxidation reactor for treating polyacrylamide wastewater.
5. The application of the biological agent for treating polyacrylamide wastewater according to claim 4, wherein in the step 1, the volume of a biological contact oxidation reactor is 75L, aeration is performed in the whole process, the dissolved oxygen of the biological contact oxidation reactor is kept at 3-4 mg/L, and the operation period is 24 h; wherein the adding amount of the packing in the biological contact oxidation reactor accounts for 40 percent of the volume of the whole reactor.
6. The application of the biological agent for treating polyacrylamide wastewater as claimed in claim 4, wherein the biological agent comprises: in the step 2, the times of adding the biological agent into the biological contact oxidation reactor are two, and the adding mode is as follows: firstly, emptying the existing liquid in a biological contact oxidation reactor, and adding the biological agent into the biological contact oxidation reactor; closing a water outlet and feeding water to the maximum volume of the reactor; thirdly, operating for 24 hours by adopting a continuous batch mode and aerating to maintain the dissolved oxygen at 2-3 mg/L; fourthly, the steps from the first step to the third step are repeated, and the second biological agent is added.
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| WO2014038216A1 (en) * | 2012-09-10 | 2014-03-13 | 三菱レイヨン株式会社 | Method for producing methacrylic acid and/or ester thereof |
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| CN101054567A (en) * | 2007-04-06 | 2007-10-17 | 哈尔滨工业大学 | Clostridium Daqing clostridium and application thereof |
| WO2014038216A1 (en) * | 2012-09-10 | 2014-03-13 | 三菱レイヨン株式会社 | Method for producing methacrylic acid and/or ester thereof |
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