CN111214519A - Extraction method of divaricate saposhnikovia root volatile oil - Google Patents
Extraction method of divaricate saposhnikovia root volatile oil Download PDFInfo
- Publication number
- CN111214519A CN111214519A CN201811404313.8A CN201811404313A CN111214519A CN 111214519 A CN111214519 A CN 111214519A CN 201811404313 A CN201811404313 A CN 201811404313A CN 111214519 A CN111214519 A CN 111214519A
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- Prior art keywords
- kettle
- extraction
- separation
- separation kettle
- divaricate saposhnikovia
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/238—Saposhnikovia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses an extraction method of divaricate saposhnikovia root volatile oil, which comprises the following steps: drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material with a 18-mesh sieve. Drying at 60 deg.C for 4 hr, precisely weighing 100g (to 0.0001g), and placing into extraction kettle. Heating the extraction kettle, the separation kettle I and the separation kettle II, and pressurizing the system by a high-pressure pump when the temperature of the extraction kettle, the separation kettle I and the separation kettle II reaches 35-40 ℃. When the pressure of the extraction kettle, the separation kettle I and the separation kettle II reaches 15-35MPa, the flow of CO2 is adjusted, and the constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from discharge ports of the separation kettle I and the separation kettle II, and respectively collecting and storing the extracts. Separating the extract with chromatography column. The extraction method of the invention is simple and easy to implement, has high yield, does not need a large amount of organic solvents, is safe and nontoxic, and is suitable for industrial production.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a method for extracting divaricate saposhnikovia root volatile oil.
Background
Radix Saposhnikoviae is root of radix Saposhnikoviae belonging to Umbelliferae and having perennial herb, and is also called GUANGFENG and DONGFENGFENG. Mainly grows in hilly areas, hillside and grass clusters, or on the sides of fields and roads, and in the middle and lower parts of high mountains. Mainly distributed in northeast, Henan, Guizhou, Hunan, Shandong, etc. Has the effects of relieving exterior syndrome, dispelling pathogenic wind, eliminating dampness, and relieving spasm, and can be used for treating common cold, headache, rheumatalgia, rubella pruritus, and tetanus. Radix Saposhnikoviae mainly contains volatile oil, polysaccharide, chromone, etc. At present, relevant reports on the extraction process of some chemical components of divaricate saposhnikovia root at home and abroad are provided, but few reports on the extraction process of the volatile oil of the divaricate saposhnikovia root are provided.
Disclosure of Invention
The invention aims to provide an extraction method of divaricate saposhnikovia root volatile oil, which has the advantages of safety, no toxicity, short time consumption, high extraction rate, simple process and continuous operation.
The invention comprises the following steps:
a. drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material with a 18-mesh sieve.
b.60 ℃ drying for 4h, accurately weighing 100g (to the accuracy of 0.0001g), and placing into an extraction kettle.
c. And (3) extraction: heating the extraction kettle, the separation kettle I and the separation kettle II, and pressurizing the system by a high-pressure pump when the temperature of the extraction kettle, the separation kettle I and the separation kettle II reaches 35-40 ℃. When the pressure of the extraction kettle, the separation kettle I and the separation kettle II reaches 15-35MPa, the flow of CO2 is adjusted, and the constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing.
d. Extraction and separation: separating the extract with chromatography column.
The extraction method of the invention is simple and easy, does not need a large amount of organic solvent, is safe and nontoxic, and is suitable for industrial production.
The specific implementation mode is as follows:
the invention is illustrated below by way of example, without the protection of the invention being restricted thereto.
Example 1:
drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material by a 18-mesh sieve; drying at 60 deg.C for 4 hr, precisely weighing 100g (to 0.0001g), placing into extraction kettle, heating extraction kettle, separation kettle I and separation kettle II, and pressurizing the system with high pressure pump when the temperature reaches 35 deg.C. When the pressures of the extraction kettle, the separation kettle I and the separation kettle II respectively reach 15MPa, adjusting CO2The constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing. Passing the obtained extract through chromatography column to obtain extract with extraction rate of 2.65%
Example 2:
drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material by a 18-mesh sieve; drying at 60 deg.C for 4 hr, precisely weighing 100g (to 0.0001g), placing into extraction kettle, heating extraction kettle, separation kettle I and separation kettle II, and pressurizing the system with high pressure pump when the temperature reaches 35 deg.C. When the pressures of the extraction kettle, the separation kettle I and the separation kettle II respectively reach 2At a pressure of 5MPa, adjusting CO2The constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing. Passing the obtained extract through a chromatographic column to obtain the extract with the extraction rate of 2.25%.
Example 3:
drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material by a 18-mesh sieve; drying at 60 deg.C for 4 hr, precisely weighing 100g (to 0.0001g), placing into an extraction kettle, heating the extraction kettle, separation kettle I and separation kettle II, and pressurizing the system with a high-pressure pump when the temperature reaches 45 deg.C. When the pressures of the extraction kettle, the separation kettle I and the separation kettle II respectively reach 25MPa, adjusting CO2The constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing. Passing the obtained extract through chromatographic column to obtain the final product with extraction rate of 2.44%.
Example 4:
drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material by a 18-mesh sieve; drying at 60 deg.C for 4 hr, precisely weighing 100g (to 0.0001g), placing into an extraction kettle, heating the extraction kettle, separation kettle I and separation kettle II, and pressurizing the system with a high-pressure pump when the temperature reaches 45 deg.C. When the pressures of the extraction kettle, the separation kettle I and the separation kettle II respectively reach 35MPa, adjusting CO2The constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing. Passing the obtained extract through a chromatographic column to obtain the extract with the extraction rate of 1.15%.
Claims (2)
1. The extraction method of the divaricate saposhnikovia root volatile oil is characterized by comprising the following steps of:
a. drying and crushing the divaricate saposhnikovia root medicinal material, and sieving the divaricate saposhnikovia root medicinal material with a 18-mesh sieve.
b.60 ℃ drying for 4h, accurately weighing 100g (to the accuracy of 0.0001g), and placing into an extraction kettle.
c. And (3) extraction: heating the extraction kettle, the separation kettle I and the separation kettle II, and pressurizing the system by a high-pressure pump when the temperature of the extraction kettle, the separation kettle I and the separation kettle II reaches 35-40 ℃. When the pressure of the extraction kettle, the separation kettle I and the separation kettle II reaches 15-35MPa, the flow of CO2 is adjusted, and the constant temperature and the constant pressure are kept. And after the selected extraction time is reached, discharging from the discharge holes of the separation kettle I and the separation kettle II, and respectively collecting and storing.
d. Extraction and separation: separating the extract with chromatography column.
2. The method for extracting ledebouriella seseloides volatile oil as claimed in claim 1, wherein the extraction and separation device in step d is a 20mm x 600mm chromatographic column with 100-200 mesh sand, and the developing agent is methylcyclohexane: ethyl acetate (5: 1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811404313.8A CN111214519A (en) | 2018-11-23 | 2018-11-23 | Extraction method of divaricate saposhnikovia root volatile oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811404313.8A CN111214519A (en) | 2018-11-23 | 2018-11-23 | Extraction method of divaricate saposhnikovia root volatile oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111214519A true CN111214519A (en) | 2020-06-02 |
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ID=70828602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811404313.8A Pending CN111214519A (en) | 2018-11-23 | 2018-11-23 | Extraction method of divaricate saposhnikovia root volatile oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111214519A (en) |
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2018
- 2018-11-23 CN CN201811404313.8A patent/CN111214519A/en active Pending
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Application publication date: 20200602 |