CN111208282B - Multi-channel mixed flow injection type perfusion microscope - Google Patents
Multi-channel mixed flow injection type perfusion microscope Download PDFInfo
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- CN111208282B CN111208282B CN202010036138.2A CN202010036138A CN111208282B CN 111208282 B CN111208282 B CN 111208282B CN 202010036138 A CN202010036138 A CN 202010036138A CN 111208282 B CN111208282 B CN 111208282B
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- 230000010412 perfusion Effects 0.000 title claims abstract description 30
- 238000004401 flow injection analysis Methods 0.000 title claims abstract description 19
- 230000005540 biological transmission Effects 0.000 claims abstract description 167
- 238000002347 injection Methods 0.000 claims abstract description 55
- 239000007924 injection Substances 0.000 claims abstract description 55
- 238000000926 separation method Methods 0.000 claims description 11
- 230000007246 mechanism Effects 0.000 claims description 9
- 230000033001 locomotion Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000011550 stock solution Substances 0.000 abstract description 13
- 239000000243 solution Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 230000009977 dual effect Effects 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 230000001771 impaired effect Effects 0.000 description 2
- 239000013618 particulate matter Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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Abstract
The invention provides a multi-channel mixed flow injection type perfusion microscope, which comprises: a flow mixer; the mixer is provided with an output port and a plurality of pairs of input ports, and the output port is used for communicating the perfusion cavity; each pair of input ports is provided with a corresponding double-channel injection structure. In the invention, each pair of input ports of the mixer corresponds to a double-channel injection structure, and stock solution is input to the input ports through two injection pumps in the double-channel injection structure. In the double-channel injection structure, piston rods of two injection pumps are driven by a driving shaft, and when the rotating speed of the driving shaft is constant, the moving speed of the piston rod of each injection pump is determined by a transmission module on a corresponding transmission station. Therefore, the injection speed of the two injection pumps can be controlled by adjusting the transmission modules on the corresponding transmission stations, so that different injection proportions are realized.
Description
Technical Field
The invention relates to the technical field of biological equipment, in particular to a multichannel mixed flow injection type perfusion microscope.
Background
The perfusion microscope mainly comprises a micro perfusion cavity on an objective table and is used for controlling a low-temperature circulating bath of which the solution in the micro perfusion cavity is in a low-temperature state. An injection syringe for injecting the solution into the micro-perfusion chamber and an extraction syringe for extracting the solution from the micro-perfusion chamber. Through the cooperation of the injection injector and the extraction injector, the dynamic balance of the solution in the micro-perfusion cavity can be kept, so that the balance of the solution pressure in the micro-perfusion cavity is ensured. Meanwhile, the perfusion microscope further comprises a device for observing cell changes in the micro-perfusion cavity, generally, the device mainly comprises a condenser, a light hole and an objective lens, light is strengthened under the converging effect of the condenser and is emitted into the micro-perfusion cavity through the light hole, the change conditions of the cells in the micro-perfusion cavity are observed after being amplified by the objective lens, and data recording can be carried out.
The existing perfusion microscope can only realize the switching of the solution from one concentration to another concentration due to only one injection syringe and one extraction syringe, namely, only the volume response of the cells under the two solutions with different concentrations can be measured. To measure the volume response of cells at another solution concentration requires changing the concentration of the solution in the syringe, which is a great inconvenience for continuous testing.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a multi-channel mixed flow injection type perfusion microscope.
The invention provides a multi-channel mixed flow injection type perfusion microscope, which comprises: a flow mixer;
the mixer is provided with an output port and a plurality of pairs of input ports, and the output port is used for communicating the perfusion cavity; each pair of input ports is provided with a corresponding double-channel injection structure;
the inner part of the mixer is provided with an isolation structure which divides the inner part of the mixer into a first chamber, a second chamber, a third chamber and a fourth chamber which are isolated from each other;
the first chamber is communicated with each input port, and the fourth chamber is communicated with the output port; the second chamber is communicated with the first chamber through a first flow guide channel on the isolation structure, the second chamber is communicated with the third chamber through a second flow guide channel on the isolation structure, and the third chamber is communicated with the fourth chamber through a third flow guide channel on the isolation structure;
the second flow guide channel is vertical to the first flow guide channel, and the third flow guide channel is vertical to the second flow guide channel;
the double-channel injection structure comprises two injection pumps and transmission mechanisms which correspond to the injection pumps one by one; the injection pump comprises a cylinder and a piston rod; the transmission mechanism includes: the device comprises a base, a support, a top plate, a driving shaft, a driving gear, a nut, a transmission support and a plurality of transmission modules; the transmission module includes: the first transmission gear, the concentric transmission gear and the second transmission gear; in the transmission module, a first transmission gear drives a concentric transmission gear to rotate at equal angles, and the concentric transmission gear drives a second transmission gear to rotate at equal strokes;
the support is arranged on the base, the top plate is arranged above the base, and the cylinder and the nut are arranged on the support and coaxially arranged; the piston rod is arranged on the top plate in a vertically sliding mode and penetrates through the corresponding nut and is in threaded connection with the nut;
the transmission support is arranged on the top plate in a vertically moving mode, the plurality of transmission modules are all arranged on the transmission support and located below the top plate, a transmission station is arranged on the motion track of the transmission support, and each transmission module sequentially passes through the transmission station in the motion process of the transmission support;
the driving shaft is rotatably arranged on the top plate, and the driving gear is sleeved on the driving shaft and rotates synchronously with the driving shaft; a first transmission gear of the transmission module is meshed with the driving gear, and a second transmission gear of the transmission module is meshed with the corresponding nut and drives the nut to rotate; in the same transmission bracket, the transmission coefficients of any two transmission modules are different.
Preferably, the first chamber, the second chamber, the third chamber and the fourth chamber are arranged in a grid shape.
Preferably, the first chamber is located above the second chamber and the fourth chamber is located above the third chamber.
Preferably, the top plate is horizontally arranged above the base through a bracket; the transmission bracket comprises a support plate and supporting plates which correspond to the transmission modules one by one; the supporting plate is horizontally arranged above the top plate, and the top plate is provided with a driving device for driving the supporting plate to move; each supporting plate is horizontally arranged above the top plate, and the supporting plate are connected through a connecting rod which penetrates through the top plate and is in sliding connection with the top plate; and a first transmission gear, a concentric transmission gear and a second transmission gear in the transmission module are rotatably arranged on the corresponding supporting plate.
Preferably, four transmission modules are arranged in the same transmission mechanism, and the ratio of the transmission coefficients of the four transmission modules is 1:2:3: 4; the transmission coefficient is the ratio of the diameter of the concentric transmission gear in the transmission module to the diameter of the first transmission gear.
Preferably, in the transmission support, the diameters of the concentric transmission gears of the transmission modules are equal.
Preferably, the driving device is a cylinder or an electric telescopic rod.
Preferably, the nut is installed on the support through a nut clamp, and the nut clamp is a notched ring with a U-shaped section and an inner diameter matched with the outer diameter of the nut.
Preferably, the cartridge is removably mounted to the holder.
Preferably, the driving shaft of each double-channel injection structure is connected with a motor for driving the driving shaft to rotate.
According to the multichannel mixed flow injection type perfusion microscope provided by the invention, the flowing direction of mixed liquid in the mixer is continuously and vertically changed in the flowing process of the mixed liquid, so that the raw liquid is gradually mixed in the flowing process. So, when guaranteeing stock solution intensive mixing, still guaranteed the flow of stock solution gently, avoid the particulate matter in the stock solution impaired.
In the invention, each pair of input ports of the mixer corresponds to a double-channel injection structure, and stock solution is input to the input ports through two injection pumps in the double-channel injection structure. In the double-channel injection structure, piston rods of two injection pumps are driven by a driving shaft, and when the rotating speed of the driving shaft is constant, the moving speed of the piston rod of each injection pump is determined by a transmission module on a corresponding transmission station. Therefore, the injection speed of the two injection pumps can be controlled by adjusting the transmission modules on the corresponding transmission stations, so that different injection proportions are realized.
Therefore, the control of the injection proportion of the input port on the mixer is realized through the structure, so that the stability and the reliability of the injection proportion of the stock solution are favorably ensured, and the quality of the mixed liquid finally input into the perfusion cavity is ensured.
Drawings
FIG. 1 is a structural diagram of a multi-channel mixed-flow injection type perfusion microscope mixer according to the present invention;
FIG. 2 is a front view of a dual channel injection structure of a multi-channel mixed flow injection perfusion microscope according to the present invention;
FIG. 3 is a partial schematic view of a dual channel injection configuration;
fig. 4 is a perspective view of a dual channel injection configuration.
The figure is as follows:
an output port A1, an input port A2, a separation structure A3, a first chamber A4, a second chamber A5, a third chamber A6, a fourth chamber A7, a first flow guide channel A8, a second flow guide channel A9 and a third flow guide channel A10;
a cylinder B1-1 and a piston rod B1-2;
the device comprises a base B2-1, a bracket B2-2, a top plate B2-3, a driving shaft B2-4, a driving gear B2-5, a nut B2-6, a supporting plate B2-7, a supporting plate B2-8, a connecting rod B2-9, a driving device B2-10, a nut clamp B2-11 and a guide rod B2-12;
a first transfer gear B3-1, a concentric transfer gear B3-2 and a second transfer gear B3-3.
Detailed Description
The invention provides a multi-channel mixed flow injection type perfusion microscope, which comprises: a flow mixer.
Referring to fig. 1, the mixer is provided with an output port a1 and a plurality of pairs of input ports a2, the output port a1 being used to communicate with the perfusion chamber. Each pair of input ports a2 is provided with a corresponding two-pass injection configuration.
The inside of the mixer is provided with a separation structure A3, and the separation structure A3 divides the inside of the mixer into a first chamber A4, a second chamber A5, a third chamber A6 and a fourth chamber A7 which are separated from each other.
The first chamber a4 communicates with each input port a2 and the fourth chamber a7 communicates with the output port a 1. The second chamber a5 is communicated with the first chamber a4 through a first flow guide channel A8 on a separation structure A3, the second chamber a5 is communicated with the third chamber A6 through a second flow guide channel a9 on a separation structure A3, and the third chamber A6 is communicated with the fourth chamber a7 through a third flow guide channel a10 on a separation structure A3.
In this way, the stock solutions input from the input ports sequentially pass through the first chamber a4, the second chamber a5, the third chamber a6 and the fourth chamber a7 and are discharged from the output port, and the stock solutions are mixed in the flowing process.
In this embodiment, the second fluid guiding passage a9 is perpendicular to the first fluid guiding passage A8, and the third fluid guiding passage a10 is perpendicular to the second fluid guiding passage a 9. Thus, the flowing direction of the mixed liquid is constantly and vertically changed during the flowing process of the mixed liquid, so that the raw liquid is gradually mixed during the flowing process. So, when guaranteeing stock solution intensive mixing, still guaranteed the flow of stock solution gently, avoid the particulate matter in the stock solution impaired.
In this embodiment, the first chamber a4, the second chamber a5, the third chamber a6 and the fourth chamber a7 are arranged in a grid shape to save space and reduce the volume of the mixer. In particular embodiments, the first chamber a4 may be positioned above the second chamber a5 and the fourth chamber a7 may be positioned above the third chamber a 6.
Referring to fig. 2, 3 and 4, the dual channel injection structure includes two injection pumps and transmission mechanisms corresponding to the injection pumps one to one. The syringe pump includes a cylinder B1-1 and a piston rod B1-2. In the double-channel injection structure, two injection pumps respectively correspond to one input port on the mixer, and the output port of the cylinder B1-1 is communicated with the corresponding input port, so that the stock solution is injected into the input port by the pushing of the piston rod B1-2.
The transmission mechanism includes: the device comprises a base B2-1, a support B2-2, a top plate B2-3, a driving shaft B2-4, a driving gear B2-5, a nut B2-6, a transmission support and a plurality of transmission modules. The transmission module includes: a first transfer gear B3-1, a concentric transfer gear B3-2 and a second transfer gear B3-3. In the transmission module, a first transmission gear B3-1 drives a concentric transmission gear B3-2 to rotate at equal angles, and a concentric transmission gear B3-2 drives a second transmission gear B3-3 to rotate at equal strokes.
Support B2-2 is mounted on base B2-1 and top panel B2-3 is mounted above base B2-1. Specifically, in this embodiment, the top panel B2-3 is mounted horizontally above the base B2-1 by a bracket B2-2.
The cylinder B1-1 and the nut B2-6 are both arranged on the bracket B2-2 and are coaxially arranged. The piston rod B1-2 is slidably mounted on the top plate B2-3 up and down, and the piston rod B1-2 passes through the corresponding nut B2-6 and is in threaded connection with the nut B2-6. Thus, when the nut B2-6 rotates, the piston rod B1-2 is driven to slide up and down, so that the piston moves.
Specifically, in the embodiment, the nut B2-6 is mounted on the bracket B2-2 through a nut clip B2-11, and the nut clip B2-11 is a notched ring with a U-shaped section and an inner diameter matched with the outer diameter of the nut B2-6, so that the nut B2-6 can rotate horizontally under the condition that the vertical position is fixed. The piston rod B1-2 can be sleeved with a guide rod B2-12 vertically installed on the top plate B2-3 so as to limit the piston rod B1-2 to rotate along with the nut B2-6 through the guide rod B2-12, and therefore the piston rod B1-2 can only slide along the guide rod B2-12 during the rotation of the nut B2-6 to realize piston movement.
In this embodiment, the cylinder B1-1 is detachably mounted on the bracket B2-2 for easy replacement. In specific implementation, a liquid injection port can be arranged at the upper part of the cylinder B1-1 so as to supplement the stock solution into the cylinder B1-1 through the liquid injection port.
The transmission support is arranged on the top plate B2-3 in a vertically moving mode, the plurality of transmission modules are all arranged on the transmission support and located below the top plate B2-3, a transmission station is arranged on the motion track of the transmission support, and each transmission module sequentially passes through the transmission station in the motion process of the transmission support.
The driving shaft B2-4 is rotatably mounted on the top plate B2-3, and the driving gear B2-5 is sleeved on the driving shaft B2-4 and rotates synchronously with the driving shaft B2-4. And a first transmission gear B3-1 of the transmission module positioned on the transmission station is meshed with a driving gear B2-5, and a second transmission gear B3-3 of the transmission module is meshed with a corresponding nut B2-6 and drives the nut B2-6 to rotate. Specifically, in this embodiment, the concentric drive gear B3-2 meshes with the second drive gear B3-3. The transmission module further comprises a third transmission gear, the third transmission gear is in transmission connection with the first transmission gear B3-1 through a gear, and the concentric transmission gear B3-2 and the third transmission gear are coaxially arranged and synchronously rotate.
In the same transmission bracket, the transmission coefficients of any two transmission modules are different.
Specifically, when the driving shaft B2-4 rotates by an angle alpha, the stroke of the driving gear B2-5 is pi d alpha, and d is the diameter of the driving gear. In the transmission process of the transmission module, the first transmission gear B3-1 has the same stroke as the driving gear B2-5, and is pi d alpha; the concentric transmission gear B3-2 has the same rotation angle as the first transmission gear B3-1, and both are
d1The diameter of the first drive gear B3-1; the stroke of the second transmission gear B3-3 is the same as that of the concentric transmission gear B3-2, and the stroke is the same as that of the concentric transmission gear B3-2
And the nut B2-6 has the same stroke as the second transmission gear B3-3. Thus, the transmission module has a transmission coefficient of
Therefore, when the transmission coefficients of the transmission modules are different, and the rotating speed of the driving shaft B2-4 is constant, different rotation angles can be realized by driving the nut B2-6 through different transmission modules, namely the piston rod B1-2 is driven to realize different movement distances.
So, through adjusting the transmission module on two transmission stations in the same binary channels injection structure, alright make two syringe pumps realize different injection rates to realize the proportion setting of stoste.
For example, in the embodiment shown in FIG. 1, the transmission ratios of the four transmission modules are:
In this example, d0-1＝d0-2＝d0-3＝d0-4，d1-1:d1-2:d1-3:d1-4＝1:2:3:4。
Thus, k1:k2:k3:k4＝4:3:2:1。
So, in this embodiment, through adjusting the transmission module on the transmission station that two syringe pumps correspond, can realize the injection ratio in 6: 1:1, 1:2, 1:3, 1:4, 2:3, 3: 4.
In the embodiment, the transmission bracket comprises a supporting plate B2-7 and supporting plates B2-8 corresponding to the transmission modules one by one. The supporting plate B2-7 is horizontally arranged above the top plate B2-3, and a driving device B2-10 for driving the supporting plate B2-7 to move is arranged on the top plate B2-3. The driving device is a cylinder or an electric telescopic rod.
Each of the pallets B2-8 is mounted horizontally above top plate B2-3, and support plate B2-7 and the pallets are connected by connecting rod B2-9 passing through top plate B2-3 and slidably connected to top plate B2-3. A first transmission gear B3-1, a concentric transmission gear B3-2 and a second transmission gear B3-3 in the transmission module are rotatably arranged on the corresponding supporting plate B2-8.
Therefore, the driving device drives the supporting plate B2-7 to move up and down, and the supporting plate B2-7 can drive the supporting plate B2-8 to move up and down through the connecting rod B2-9, so that the transmission module on the transmission station is switched.
In the embodiment, a motor for driving the driving shaft B2-4 in each double-channel injection structure is connected with each driving shaft B2-4. Therefore, the rotating speed control of the driving shaft in different double-channel injection structures is realized, and more proportion regulation and control are met. In specific implementation, the driving shafts B2-4 of the adjacent double-channel injection structures can be in transmission connection through a gear transmission mechanism, so that all the double-channel injection structures are driven to work through one motor, and stable control of injection proportion is guaranteed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (8)
1. A multi-channel mixed flow injection type perfusion microscope, comprising: a flow mixer;
the mixer is provided with an output port (A1) and a plurality of pairs of input ports (A2), and the output port (A1) is used for communicating the perfusion cavity; each pair of input ports (A2) is provided with a corresponding double-channel injection structure;
the inside of the mixer is provided with a separation structure (A3), and the separation structure (A3) divides the inside of the mixer into a first chamber (A4), a second chamber (A5), a third chamber (A6) and a fourth chamber (A7) which are separated from each other;
the first chamber (a4) communicates with each inlet port (a2), and the fourth chamber (a7) communicates with the outlet port (a 1); the second chamber (A5) is communicated with the first chamber (A4) through a first flow guide channel (A8) on a separation structure (A3), the second chamber (A5) is communicated with the third chamber (A6) through a second flow guide channel (A9) on a separation structure (A3), and the third chamber (A6) is communicated with the fourth chamber (A7) through a third flow guide channel (A10) on the separation structure (A3);
the second flow guide channel (A9) is vertical to the first flow guide channel (A8), and the third flow guide channel (A10) is vertical to the second flow guide channel (A9);
the double-channel injection structure comprises two injection pumps and transmission mechanisms which correspond to the injection pumps one by one; the syringe pump comprises a cylinder body (B1-1) and a piston rod (B1-2); the transmission mechanism includes: the device comprises a base (B2-1), a bracket (B2-2), a top plate (B2-3), a driving shaft (B2-4), a driving gear (B2-5), a nut (B2-6), a transmission bracket and a plurality of transmission modules; the transmission module includes: a first transmission gear (B3-1), a concentric transmission gear (B3-2) and a second transmission gear (B3-3); in the transmission module, a first transmission gear (B3-1) drives a concentric transmission gear (B3-2) to rotate at the same angle, and the concentric transmission gear (B3-2) drives a second transmission gear (B3-3) to rotate at the same stroke;
the bracket (B2-2) is arranged on the base (B2-1), the top plate (B2-3) is arranged above the base (B2-1), and the cylinder (B1-1) and the nut (B2-6) are both arranged on the bracket (B2-2) and are coaxially arranged; the piston rod (B1-2) is installed on the top plate (B2-3) in a vertically sliding mode, and the piston rod (B1-2) penetrates through the corresponding nut (B2-6) and is in threaded connection with the nut;
the transmission support is arranged on the top plate (B2-3) in a vertically moving mode, the plurality of transmission modules are all arranged on the transmission support and located below the top plate (B2-3), a transmission station is arranged on the motion trail of the transmission support, and each transmission module sequentially passes through the transmission station in the motion process of the transmission support;
the driving shaft (B2-4) is rotatably arranged on the top plate (B2-3), and the driving gear (B2-5) is sleeved on the driving shaft (B2-4) and rotates synchronously with the driving shaft (B2-4); a first transmission gear (B3-1) of the transmission module positioned on the transmission station is meshed with a driving gear (B2-5), and a second transmission gear (B3-3) of the transmission module is meshed with a corresponding nut (B2-6) and drives the nut (B2-6) to rotate; in the same transmission bracket, the transmission coefficients of any two transmission modules are different;
the multichannel mixed flow injection type perfusion microscope is characterized in that a first chamber (A4), a second chamber (A5), a third chamber (A6) and a fourth chamber (A7) are arrayed in a Chinese character tian shape;
the multichannel mixed flow injection perfusion microscope is characterized in that a first chamber (A4) is positioned above a second chamber (A5), and a fourth chamber (A7) is positioned above a third chamber (A6).
2. The multichannel mixed flow injection type perfusion microscope according to claim 1, wherein the top plate (B2-3) is horizontally mounted above the base (B2-1) by a bracket (B2-2); the transmission bracket comprises a supporting plate (B2-7) and supporting plates (B2-8) which correspond to the transmission modules one by one; the supporting plate (B2-7) is horizontally arranged above the top plate (B2-3), and the top plate (B2-3) is provided with a driving device (B2-10) for driving the supporting plate (B2-7) to move; each supporting plate (B2-8) is horizontally arranged above the top plate (B2-3), and the supporting plate (B2-7) and the supporting plate are connected through a connecting rod (B2-9) which penetrates through the top plate (B2-3) and is in sliding connection with the top plate (B2-3); a first transmission gear (B3-1), a concentric transmission gear (B3-2) and a second transmission gear (B3-3) in the transmission module are rotatably installed on corresponding supporting plates (B2-8).
3. The multichannel mixed-flow injection perfusion microscope of claim 2, wherein four transmission modules are provided in the same transmission mechanism, and the ratio of the transmission coefficients of the four transmission modules is 1:2:3: 4; the transmission coefficient is the ratio of the diameter of the concentric transmission gear (B3-2) in the transmission module to the diameter of the first transmission gear (B3-1).
4. A multichannel mixed flow injection perfusion microscope according to claim 3, characterised in that the diameters of the concentric transmission gears (B3-2) of the transmission modules in the transmission frame are all equal.
5. The multichannel mixed flow injection perfusion microscope of claim 2, wherein the driving device is a pneumatic cylinder or an electric telescopic rod.
6. A multichannel mixed flow injection type perfusion microscope according to claim 1, characterized in that the nut (B2-6) is mounted on the stent (B2-2) by a nut clip (B2-11), the nut clip (B2-11) being a notched ring with a U-shaped cross section and an inner diameter matching the outer diameter of the nut (B2-6).
7. A multichannel mixed flow injection type perfusion microscope according to claim 1, wherein the cartridge (B1-1) is detachably mounted on the support (B2-2).
8. A multichannel francis injection type perfusion microscope according to claim 1, wherein the drive shaft (B2-4) of each two-channel injection configuration is connected to a motor for driving it in rotation.
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