CN111202755A - Application of tricholoma matsutake extract - Google Patents
Application of tricholoma matsutake extract Download PDFInfo
- Publication number
- CN111202755A CN111202755A CN202010154930.8A CN202010154930A CN111202755A CN 111202755 A CN111202755 A CN 111202755A CN 202010154930 A CN202010154930 A CN 202010154930A CN 111202755 A CN111202755 A CN 111202755A
- Authority
- CN
- China
- Prior art keywords
- extract
- tricholoma matsutake
- antioxidant
- filter membrane
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Toxicology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to the technical field of medicines and cosmetics, and discloses application of a tricholoma matsutake extract. The present invention provides the use of matsutake mushroom extract for improving the capacity of endogenous antioxidant system in vivo, can repair the damage of skin after photodamage/photoaging and treat diseases, tissue aging and tissue function reduction caused by G6PD deficiency, and can be applied to the preparation of G6PD enhancing agent or antioxidant.
Description
Technical Field
The invention relates to the technical field of medicines and cosmetics, in particular to application of tricholoma matsutake extract.
Background
Glucose-6-phosphate dehydrogenase (G6PD) is widely present in animals, distributed in the cytoplasm of all cells of the human body, including skin cells. G6PD is a key enzyme of the pentose phosphate pathway, catalyzes the oxidation of glucose-6-phosphate to gluconolactone-6-phosphate, and simultaneously reduces NADP + to NADPH for biosynthesis and maintenance of the reduced state in the cell. Studies have shown that G6PD plays an important role during oxidative stress. It is activated and catalyzes the production of NADPH, which is mainly used for many biochemical reactions such as reductive biosynthesis, glutathione reduction, detoxification, and oxidase-mediated superoxide production, etc., to maintain the intracellular redox state equilibrium. Therefore, the level of glucose-6-phosphate dehydrogenase can reflect the biosynthesis and antioxidant capacity of organisms to some extent.
In the absence of G6PD, red blood cells are easily destroyed and acute hemolytic reaction occurs when the body contacts certain substances with oxidation or takes such drugs. Fava is a type of G6PD deficiency, manifested by hemolytic anemia arising from fava bean consumption. The specific mechanism of hemolysis is unknown, and only a few people with G6PD deficiency in the same region develop the disease, and the disease does not develop every year when broad beans are eaten. To date, few enhancers having the effect of increasing the expression of G6PD have been reported in the literature.
Disclosure of Invention
In view of the above, an object of the present invention is to provide use of matsutake Mushroom extract for preparing glucose-6-phosphate dehydrogenase (G6PD) enhancer;
another object of the present invention is to provide the use of the matsutake mushroom extract for preparing an antioxidant;
tricholoma Matsutake (Tricholoma Matsutake S.Ito et Imai)]Sing), a scientific name of Tricholoma matsutake, is one of edible fungi, and contains various physiologically active substances, such as protein, crude fat, crude fiber, and vitamin B1Vitamin B2Vitamin C, nicotinic acid, various amino acids, polysaccharide, unsaturated fatty acid, nucleic acid derivatives, peptide substances, trace elements and the like. It has high nutritive value, high medicinal value, and can resist bacteria, fungi, cancer, allergy, oxidation, enhance immunity and prevent premature aging. At present, no report is found about the influence of the tricholoma matsutake on the endogenous antioxidant system of human body such as glucose-6-phosphate dehydrogenase (G6PD) at home and abroad.
In a mouse test, compared with a normal control group, a UVB induced skin damage model group and a vitamin C group, the tricholoma matsutake extract group can remarkably promote the expression of G6PD in skin tissues, has obviously improved content, and can be applied to diseases, tissue aging and tissue function reduction caused by G6PD deficiency; in view of the fact that the tricholoma matsutake extract can remarkably enhance the expression effect of G6PD, the invention further verifies the ROS removing effect, compared with a normal control group, a UVB induced skin damage model group and a vitamin C group, the tricholoma matsutake extract group can remarkably reduce the content of ROS, has obvious antioxidation effect, and can be applied to repair of skin damage caused by light injury/photoaging.
Based on the technical effects, the invention provides the application of the tricholoma matsutake extract in preparing the glucose-6-phosphate dehydrogenase (G6PD) enhancer; and the use of a matsutake mushroom extract for the preparation of an antioxidant, which in an embodiment of the invention is an anti-ROS oxidizing agent.
Meanwhile, according to the application, the invention also provides a G6PD enhancer or antioxidant, which comprises tricholoma matsutake extract, wherein the content of the tricholoma matsutake extract is at least 0.1% w/w, more preferably at least 0.42% w/w, and further preferably 0.42% w/w-20% w/w; in a specific embodiment of the invention, the content of the matsutake mushroom extract is 0.42% w/w or 4.2% w/w.
The G6PD enhancer or antioxidant is preferably in the form of paste or liquid, and can also contain other suitable active ingredients and/or carrier auxiliary materials besides the tricholoma matsutake extract as the active ingredient, wherein the carrier auxiliary materials are pharmaceutically, cosmetically or cosmetically acceptable carrier auxiliary materials, including but not limited to diluents, excipients, fillers, binders, wetting agents, disintegrants, absorption promoters, adsorption carriers, surfactants and lubricants, and any one or more of the above materials can be selected. In a specific embodiment of the present invention, the carrier vehicle is glycerol and water; or stearic acid, potassium hydroxide, glycerol and deionized water. The other suitable active ingredients are other natural products and chemicals which do not have antagonistic reaction with the matsutake mushroom extract.
Preferably, the tricholoma matsutake extract is prepared by the following method:
pulverizing Tricholoma matsutake, extracting with ethanol water solution by soaking and heating, filtering conventionally, and collecting primary filtrate; after cooling, filtering with a first filter membrane to obtain a secondary filtrate; filtering the secondary filtrate with a second filter membrane to obtain a third filtrate; concentrating the filtrate, and drying to obtain Tricholoma matsutake extract. Wherein the aperture of the filter membrane filtered by the second filter membrane is smaller than that of the filter membrane filtered by the first filter membrane.
Wherein the pulverization is to pulverize Tricholoma matsutake by a pulverizer, and the preferable fineness is required to be 10 meshes or less.
The pulverized tricholoma matsutake is preferably extracted by adding 70% ethanol aqueous solution according to the weight ratio of 1: 5-1: 20, namely, 5 g-20 g of 70% ethanol aqueous solution is added into 1g of pulverized tricholoma matsutake.
In some embodiments, the Tricholoma matsutake may be pulverized and extracted with 70% ethanol aqueous solution at a weight ratio of 1: 10.
The water in the ethanol aqueous solution is preferably distilled water. The extraction time is preferably at least 30min, more preferably 120-360min, and in the embodiment of the present invention 240 min.
The preparation method of the tricholoma matsutake extract comprises the steps of extracting and filtering through ethanol to obtain primary filtrate, namely tricholoma matsutake extract, and then filtering through a filter membrane twice. Preferably, filtering the mixed solution by a filter membrane of 1-5 mu m to obtain secondary filtrate; and filtering the secondary filtrate by a filter membrane of 0.1-0.65 mu m to obtain a tertiary filtrate.
In some embodiments, the matsutake Mushroom extract is first filtered through a 5 μm filter to obtain a secondary filtrate; the second filtrate was then filtered through a 0.25 μm filter to obtain a third filtrate.
In the preparation method of the tricholoma matsutake extract, the tricholoma matsutake extract is obtained by concentrating and drying the three filtrates. Preferably, the drying is vacuum freeze drying.
According to the above technical scheme, the present invention provides the use of matsutake mushroom extract for improving the capacity of endogenous antioxidant system, repairing the damage of skin after photodamage/photoaging and treating diseases, tissue aging and tissue function reduction caused by G6PD deficiency, and can be applied to the preparation of G6PD enhancing agent or antioxidant.
Drawings
FIG. 1 shows the result of quantitative determination of the expression level of G6PD in skin tissue by glucose 6 phosphate dehydrogenase (G6PD) kit; wherein, the difference comparison among the groups is performed by One-Way analysis of variance (One-Way ANOVA), compared with a blank group: # p < 0.05; compared to the model set: p < 0.05, p < 0.01;
FIG. 2 shows the quantitative determination of ROS levels in skin tissues by the ROS kit; wherein, the difference comparison among the groups is performed by One-Way analysis of variance (One-Way ANOVA), compared with a blank group: # p < 0.05; compared to the model set: p < 0.05, p < 0.01.
Detailed Description
The invention discloses application of tricholoma matsutake extracts, and the technical parameters can be properly improved by taking the contents of the tricholoma matsutake extracts as reference by the technical personnel in the field. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the invention has been described in terms of preferred embodiments, it will be apparent to those skilled in the art that the techniques of the invention can be implemented and applied by modifying or appropriately combining the applications described herein without departing from the spirit, scope and spirit of the invention.
In a specific embodiment of the present invention, the tricholoma matsutake extract is extracted according to the following method:
pulverizing the tricholoma matsutake, adding 70% ethanol water solution according to the weight ratio of 1: 5-1: 20, extracting for 240 minutes at 20-60 ℃ by adopting a soaking heating method, filtering conventionally and collecting primary filtrate; cooling, and filtering by a filter membrane of 1-5 mu m to obtain secondary filtrate; filtering the secondary filtrate by a filter membrane of 0.1-0.65 mu m to obtain a tertiary filtrate; concentrating the filtrate, and drying to obtain Tricholoma matsutake extract.
The application of the matsutake mushroom extract provided by the invention is further explained below.
Example 1: extracting Tricholoma matsutake extract
Pulverizing Tricholoma matsutake, weighing 50g of powder, adding 70% ethanol water solution according to the weight ratio of 1:10, extracting for 240min at 20-60 deg.C by soaking heating method, filtering conventionally, and collecting primary filtrate; cooling, and filtering by a filter membrane of 1-5 mu m to obtain secondary filtrate; filtering the secondary filtrate by a filter membrane of 0.1-0.65 mu m to obtain a tertiary filtrate; concentrating the filtrate, and freeze drying to obtain 3.68g Tricholoma matsutake extract lyophilized powder.
Example 2: g6PD enhancement test
Weighing 1.25g of tricholoma matsutake extract, adding the tricholoma matsutake extract into 28.25g of cream matrix (comprising the following components, by mass, 20.0% of stearic acid, 1.25% of potassium hydroxide (80% w/w), 10.0% of glycerol and 68.75% of deionized water), uniformly mixing to prepare 4.2% w/w tricholoma matsutake extract cream, and preparing a paste with the content of the tricholoma matsutake extract of 0.42% w/w by using the cream matrix; vitamin C125mg was weighed and added to 29.38g cream base to make up 0.42% w/w ready for use.
SPF-grade Kunming mice, 24 female mice with the age of 6-7 weeks (18-22 g, Guangdong university of traditional Chinese medicine), and 5 female mice are divided into a normal group, a UVB irradiation group, a vitamin C group, a tricholoma matsutake extract high-dose protection group and a tricholoma matsutake extract low-dose protection group, wherein the groups are 5 groups. The back skin of each group of mice was dehaired and the fuzz was removed with 6% w/w aqueous sodium sulfide solution, the back skin was smeared with vaseline alone daily in the normal group, and UVB (302nm) irradiation was performed daily in each of the other groups except the normal group to establish a UVB-induced skin damage model. The initial intensity of the first week ultraviolet light is the Minimum Erythema Dose (MED), and the irradiation dose is 100mj/cm2Light source about 10cm high for week 4, 4 Minimal Erythemal Doses (MED), 5 times per week (except Saturday, day), 400mj/cm for the remaining few cycles2Once per time. The whole radiation dose is about 13J/cm2The whole experimental period is 8 weeks; 2H before UVB irradiation, 118mg/1 time/day is coated on the back according to groups, the concentration of the tricholoma matsutake extract is 4.2% w/w and 0.42% w/w respectively, and 118mg of cream matrix is coated on a normal control group and a model group.
G6PD enzyme activity test:
taking a proper amount of skin tissue which is placed at minus 80 ℃, and freezing and thawing on ice. 1mL of pre-cooled phosphate buffer solution was added per 100mg of skin tissue, the tissue was ground using a tissue grinder and centrifuged at 10000g for 10 minutes at 4 ℃ and all samples were calibrated for protein using the bicinchoninic acid (BCA) method.
A part of the total supernatant of the above samples was used for the assay of mouse skin glucose-6-phosphate dehydrogenase (G6PD) kit (Beijing Solaibao).
The determination result is shown in fig. 1, and the tricholoma matsutake extract can remarkably promote the expression of G6PD, and the content of the tricholoma matsutake extract is remarkably higher than that of a normal control group, a model group and a vitamin C group.
Example 3: ROS scavenging assay
Weighing 1.25g of matsutake extract, adding 12.5mL of glycerol and 12.5mL of water, mixing well to prepare a glycerol aqueous solution of 4.2% w/w matsutake extract, taking part of the solution, diluting with the glycerol aqueous solution to 0.42% w/w, weighing vitamin C125mg, adding 12.5mL of glycerol and 12.5mL of water, mixing well to prepare 0.42% w/w for later use.
SPF-grade Kunming mice, 25 female mice with the age of 6-7 weeks (18-22 g, Guangdong university of traditional Chinese medicine), and 5 female mice are divided into a normal group, a UVB group, a vitamin C group, a tricholoma matsutake extract high-dose protection group and a tricholoma matsutake extract low-dose protection group, wherein the groups are 5 groups. The back skin of each group of mice was dehaired and the villi removed with 6% w/w aqueous sodium sulfide solution, the normal group was applied to the back skin only daily with aqueous glycerol solution, and the rest groups except the normal group were irradiated with UVB (302nm) daily to establish a UVB-induced skin damage model. The initial intensity of the first week ultraviolet light is the Minimum Erythema Dose (MED), and the irradiation dose is 100mj/cm2Light source about 10cm high for week 4, 4 Minimal Erythemal Doses (MED), 5 times per week (except Saturday, day), 400mj/cm for the remaining few cycles2Once per time. The whole radiation dose is about 13J/cm2The whole experimental period is 8 weeks; 2H before UVB irradiation, the back is coated with 100L/1 times/day according to groups, the dosage concentration of the tricholoma matsutake extract is 164mM (or 4.2% w/w) and 16.4mM (or 0.42% w/w), the dosage concentration of the vitamin C group is 15mM (or 0.42% w/w), and the normal control group and the model group are coated with 100L of the same glycerin and water solvent.
ROS enzyme linked immunosorbent assay (Elisa) assay:
taking a proper amount of skin tissue which is placed at minus 80 ℃, and freezing and thawing on ice. Adding 1mL of precooled phosphate buffer solution into every 100mg of skin tissue, performing tissue grinding treatment by using a tissue grinder, centrifuging at the temperature of 4 ℃ for 10 minutes at 10000g, and using partial sample total supernatant for measuring the content of mouse ROS factor enzyme linked immunosorbent assay kit (Jiangsu enzyme label).
The determination result is shown in fig. 2, and the tricholoma matsutake extract can obviously reduce the content of ROS, and is superior to a normal control group, a model group and a vitamin C group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (9)
1. Use of Tricholoma matsutake extract in preparing glucose-6-phosphate dehydrogenase enhancer is provided.
2. Use of Tricholoma matsutake extract in preparing antioxidant is provided.
3. The use of claim 2, wherein the antioxidant is an anti-ROS oxidant.
4. The use according to claim 1 or 2, wherein the extract of tricholoma matsutake is an ethanol extract of tricholoma matsutake.
5. The use according to claim 1 or 2, wherein the tricholoma matsutake extract is prepared according to the following method:
pulverizing Tricholoma matsutake, extracting with ethanol water solution by soaking and heating, filtering conventionally, and collecting primary filtrate; after cooling, filtering with a first filter membrane to obtain a secondary filtrate; filtering the secondary filtrate with a second filter membrane to obtain a third filtrate; concentrating the filtrate, and drying to obtain Tricholoma matsutake extract; wherein the aperture of the filter membrane filtered by the second filter membrane is smaller than that of the filter membrane filtered by the first filter membrane.
6. A glucose-6-phosphate dehydrogenase enhancer or antioxidant comprises an extract of Tricholoma matsutake.
7. An enhancer or antioxidant as claimed in claim 6 wherein the matsutake mushroom extract is present in an amount of at least 0.1% w/w.
8. The enhancer or antioxidant as claimed in claim 6 or 7, further comprising other suitable active ingredients and/or carrier excipients, wherein the carrier excipients are pharmaceutically, cosmetically or cosmetically acceptable carrier excipients.
9. The enhancer or antioxidant according to claim 8, wherein the carrier vehicle is selected from one or more of diluents, excipients, fillers, binders, wetting agents, disintegrants, absorption enhancers, adsorptive carriers, surfactants and lubricants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010154930.8A CN111202755A (en) | 2020-03-06 | 2020-03-06 | Application of tricholoma matsutake extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010154930.8A CN111202755A (en) | 2020-03-06 | 2020-03-06 | Application of tricholoma matsutake extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111202755A true CN111202755A (en) | 2020-05-29 |
Family
ID=70781719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010154930.8A Pending CN111202755A (en) | 2020-03-06 | 2020-03-06 | Application of tricholoma matsutake extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111202755A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516183A (en) * | 2020-12-22 | 2021-03-19 | 长春理工大学 | Preparation method and application of extract with repair effect on trauma |
| CN116421482A (en) * | 2023-04-20 | 2023-07-14 | 稀物集(广州)生物科技有限公司 | Composition containing tricholoma matsutake extract with anti-aging effect and application thereof |
| CN116531280A (en) * | 2023-05-06 | 2023-08-04 | 稀物集(广州)生物科技有限公司 | Tricholoma matsutake extract with anti-aging effect and preparation method thereof |
-
2020
- 2020-03-06 CN CN202010154930.8A patent/CN111202755A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 尹秀莲等: "松茸不同溶剂提取物及其抗氧化活性", 《食品与发酵工程》 * |
| 王楠等: "松茸化妆品的美白功效评价", 《日用化学工业》 * |
| 黄俊丽等: "松茸、黑牛肝菌、双孢白蘑菇提取物体外抗氧化性试验研究", 《食品与生物技术学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516183A (en) * | 2020-12-22 | 2021-03-19 | 长春理工大学 | Preparation method and application of extract with repair effect on trauma |
| CN116421482A (en) * | 2023-04-20 | 2023-07-14 | 稀物集(广州)生物科技有限公司 | Composition containing tricholoma matsutake extract with anti-aging effect and application thereof |
| CN116421482B (en) * | 2023-04-20 | 2023-11-21 | 稀物集(广州)生物科技有限公司 | Composition containing tricholoma matsutake extract with anti-aging effect and application thereof |
| CN116531280A (en) * | 2023-05-06 | 2023-08-04 | 稀物集(广州)生物科技有限公司 | Tricholoma matsutake extract with anti-aging effect and preparation method thereof |
| CN116531280B (en) * | 2023-05-06 | 2024-01-09 | 稀物集(广州)生物科技有限公司 | Tricholoma matsutake extract with anti-aging effect and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Andallu et al. | Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats | |
| CN111202755A (en) | Application of tricholoma matsutake extract | |
| EP2123252B1 (en) | Method for increasing hair growth | |
| KR101275971B1 (en) | Composition for antioxidation effect comprising sparassis crispa extract | |
| JPH09227398A (en) | Antiobese agent | |
| KR101667284B1 (en) | Cosmetics composition containing a fermented extract of black ginseng and herbal mixture | |
| JP7213307B2 (en) | Composition for activating protein L-isoaspartate methyltransferase | |
| TWI311469B (en) | Legume products | |
| KR20130088997A (en) | Cosmetic composition for hair growth and restoration from caviar extracts and its fermentation | |
| CN115887518A (en) | Anti-glycation application of sweet wormwood herb extract | |
| KR100549057B1 (en) | Cosmetic composition containing mixture extract of OUGIDANPaecilomyces japonica, Acanthopanax senticosus, Ganoderam Lucidum, Deer antlers and Ginseng, and the method of manufacturing thereof with supercritical fluids extraction | |
| KR20190116837A (en) | Composition Comprising Radish Extract for Improving Skin Wrinkle and Elasticity | |
| US7713557B2 (en) | Natural oriental medicinal composition for the promotion of hair growth and method of preparing the same | |
| JP2955126B2 (en) | Pharmaceutical oleaginous preparation, food oleaginous preparation and production method thereof | |
| KR101991926B1 (en) | Composition for improving skin moisturization comprising royal jelly | |
| Mathur et al. | Therapeutic use of wheat grass juice for the treatment of anemia in young women of Ajmer city (Rajasthan, India) | |
| KR101601024B1 (en) | Composition for skin external application containing the extract of red pine roots | |
| US6908614B2 (en) | Anti-aging/menopause symptoms relief using ganoderma lucidum spores | |
| KR100550165B1 (en) | Manufacturing method of water-soluble propolis | |
| CA2240997A1 (en) | Use of ginkgo biloba extracts for the preparation of pharmaceutical compositions, and method of cosmetic treatment using a ginkgo biloba extract | |
| KR101558184B1 (en) | Anti-aging composition containing red-grape-leaf extract berrylike mixtures and selenium | |
| CN107362245A (en) | It is a kind of that there is the anti-oxidant and composition of reducing homosysteine | |
| Kim et al. | Preventive effect of fermented Gelidium amansii and Cirsium japonicum extract mixture against UVB-induced skin photoaging in hairless mice | |
| KR20210157987A (en) | Food composition for relieving hangover and improving hepatic function | |
| CN105287275A (en) | Cosmetic composition containing songaria cynomorium herb |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200529 |
|
| RJ01 | Rejection of invention patent application after publication |