CN111139180A - Culture protection system of intestinal flora - Google Patents
Culture protection system of intestinal flora Download PDFInfo
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- CN111139180A CN111139180A CN202010014738.9A CN202010014738A CN111139180A CN 111139180 A CN111139180 A CN 111139180A CN 202010014738 A CN202010014738 A CN 202010014738A CN 111139180 A CN111139180 A CN 111139180A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H50/00—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
- G16H50/30—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for calculating health indices; for individual health risk assessment
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H50/00—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
- G16H50/70—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for mining of medical data, e.g. analysing previous cases of other patients
Abstract
The invention belongs to the technical field of culture of intestinal flora, and discloses a culture protection system of intestinal flora, which comprises: the system comprises a culture video acquisition module, a temperature detection module, a bacteria detection module, a main control module, a disinfection module, a sequencing module, a normal-temperature storage module, a flora evaluation module and a display module. According to the invention, an intestinal flora sequencing report can be accurately obtained through a sequencing module; meanwhile, the preservation solution provided by the normal-temperature preservation module can be used for preserving and transporting the intestinal flora sample at normal temperature; immersing the collected intestinal flora sample in a preservation solution, and maintaining the stability of the microbial structure composition in the sample under the normal temperature condition; therefore, the sample collection and storage can be completed by the examinee at any time, and the normal-temperature transportation of the sample is convenient; has great market prospect and economic value.
Description
Technical Field
The invention belongs to the technical field of culture of intestinal flora, and particularly relates to a culture protection system of the intestinal flora.
Background
The intestinal flora and normal microorganisms in the human intestinal tract, such as bifidobacterium, lactobacillus and the like can synthesize various vitamins necessary for the growth and development of human bodies, such as B vitamins (vitamin B1, B2, B6, B12), vitamin K, nicotinic acid, pantothenic acid and the like, can also utilize protein residues to synthesize essential amino acids, such as aspartic acid, phenylalanine, valine, threonine and the like, participate in the metabolism of saccharides and proteins, and can promote the absorption of mineral elements such as iron, magnesium, zinc and the like. These nutrients have an important role in human health and cause various diseases if they are lacking. However, the existing culture protection system for the intestinal flora has inaccurate sequencing on the intestinal flora; meanwhile, the intestinal flora is not beneficial to being preserved at normal temperature.
In summary, the problems of the prior art are as follows: the existing culture protection system of the intestinal flora has inaccurate sequencing on the intestinal flora; meanwhile, the intestinal flora is not beneficial to being preserved at normal temperature.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a culture protection system for intestinal flora.
The invention is realized in this way, and a system for culturing and protecting intestinal flora comprises:
the system comprises a culture video acquisition module, a temperature detection module, a bacteria detection module, a main control module, a disinfection module, a sequencing module, a normal-temperature storage module, a flora evaluation module and a display module;
the culture video acquisition module is connected with the main control module and is used for acquiring culture video data of the intestinal flora through the camera;
the temperature detection module is connected with the main control module and used for detecting the culture environment temperature data of the intestinal flora through a temperature sensor;
the bacteria detection module is connected with the main control module and used for detecting the bacteria information of the culture environment of the intestinal flora through the bacteria detector;
the main control module is connected with the culture video acquisition module, the temperature detection module, the bacteria detection module, the disinfection module, the sequencing module, the normal temperature storage module, the flora evaluation module and the display module and is used for controlling the normal work of each module through a host;
the disinfection module is connected with the main control module and is used for disinfecting the culture environment of the intestinal flora through disinfection equipment;
the sequencing module is connected with the main control module and used for sequencing the intestinal flora;
the normal-temperature preservation module is connected with the main control module and is used for preserving the intestinal flora at normal temperature through preservation liquid;
the flora evaluation module is connected with the main control module and used for evaluating the growth state of the intestinal flora through an evaluation program;
and the display module is connected with the main control module and used for displaying the acquired culture video, temperature, bacteria information and the evaluation result of the growth state of the intestinal flora through a display.
Further, the sequencing module comprises the following sequencing method:
(1) constructing one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model through a database program; constructing an intestinal flora functional gene database;
(2) detecting the intestinal flora structure in the sample, and comparing the intestinal flora structure in the sample with one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model;
(3) detecting functional genes of intestinal flora in the sample, and comparing the functional genes with the functional gene database data of the flora; and generating an intestinal flora sequencing report.
Further, the construction method of the flora age database comprises the following steps: acquiring a certain number of intestinal flora samples of healthy people of 0-100 years of age in each age group, testing the total flora amount, the total lactobacillus amount, the total bifidobacterium amount and the total escherichia coli amount of each sample, and constructing the flora structure related information of each age group to form the related relation between the age and the flora structure.
Further, the construction method of the flora trend model comprises the following steps: obtaining a certain number of intestinal flora samples of people of different health conditions of 0-100 years of age, classifying the flora structures of the age and the health conditions into a plurality of flora trends according to the healthy trend.
Further, the flora trend includes a very healthy trend, a high healthy trend, a relatively healthy trend, an aging trend, a sub-healthy trend, a disease trend of grade 1, a disease trend of grade 2, a disease trend of grade 3.
Further, the construction method of the risk prediction trend model comprises the following steps: obtaining intestinal flora samples of a certain number of people with different diseases and different degrees of the same disease, typing the flora structures of the age group and the disease condition to form a correlation between the diseases and the disease degree, and classifying the disease degree into low, medium, high and high degrees, wherein the diseases comprise diabetes, colorectal cancer, ulcerative colitis, obesity and cardiovascular diseases.
Further, the construction method of the nutrient absorption level model comprises the following steps: acquiring a certain number of intestinal flora samples of crowds with different food nutrient absorption levels, classifying the flora structure of each food with different nutrient absorption levels to form a correlation between the flora structure and each food nutrient absorption level, and classifying the nutrient absorption levels into low, medium, high and high degrees; the different foods comprise sugar, grains, rice, flour foods, meat, egg and milk foods and fat foods.
Further, the component method of the antibiotic resistance model comprises the following steps: the method comprises the steps of obtaining intestinal flora samples of healthy people eating antibiotics for a certain time, typing flora structures to form a correlation between the flora structures and the healthy people eating the antibiotics for a certain time, and classifying antibiotic resistance into low, medium, high and high degrees.
Further, the preparation method of the storage solution in the normal-temperature storage module is as follows:
1) selecting a cason preservative, disodium ethylene diamine tetraacetate, Tris-HCl buffer solution, lithium chloride, ethanol and sterile water; the kasong preservative accounts for 0.5 per mill of the total mass of the preservation solution; the molar fraction of the ethylene diamine tetraacetic acid disodium is 10 mM; the molar volume fraction of the Tris-HCl buffer solution is 10 mM; the molar volume fraction of the lithium chloride is 2 mol/L; the volume part of the ethanol is 95 percent; the volume fraction of the sterile water is 2%;
2) respectively weighing corresponding parts by mass of a cason preservative, and corresponding mole fractions of disodium ethylenediaminetetraacetate and lithium chloride in TrisHCl buffer solution, dissolving in corresponding volumes of ethanol, uniformly mixing, adding sterile water, and storing at normal temperature to obtain the preservation solution.
The invention has the advantages and positive effects that: according to the invention, an intestinal flora sequencing report can be accurately obtained through a sequencing module; meanwhile, the preservation solution provided by the normal-temperature preservation module can be used for preserving and transporting the intestinal flora sample at normal temperature; immersing the collected intestinal flora sample in a preservation solution, and maintaining the stability of the microbial structure composition in the sample under the normal temperature condition; therefore, the sample collection and storage can be completed by the examinee at any time, and the normal-temperature transportation of the sample is convenient; has great market prospect and economic value.
Drawings
Fig. 1 is a block diagram of a system for culturing and protecting intestinal flora according to an embodiment of the present invention.
In the figure: 1. a culture video acquisition module; 2. a temperature detection module; 3. a bacteria detection module; 4. a main control module; 5. a sterilization module; 6. a sequencing module; 7. a normal temperature preservation module; 8. a flora evaluation module; 9. and a display module.
Detailed Description
In order to further understand the contents, features and effects of the present invention, the following embodiments are illustrated and described in detail with reference to the accompanying drawings.
The structure of the present invention will be described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the system for protecting and culturing intestinal flora according to the embodiment of the present invention includes: the system comprises a culture video acquisition module 1, a temperature detection module 2, a bacteria detection module 3, a main control module 4, a disinfection module 5, a sequencing module 6, a normal-temperature storage module 7, a flora evaluation module 8 and a display module 9.
The culture video acquisition module 1 is connected with the main control module 4 and is used for acquiring culture video data of intestinal flora through a camera;
the temperature detection module 2 is connected with the main control module 4 and used for detecting the culture environment temperature data of the intestinal flora through a temperature sensor;
the bacteria detection module 3 is connected with the main control module 4 and is used for detecting the bacteria information of the culture environment of the intestinal flora through a bacteria detector;
the main control module 4 is connected with the culture video acquisition module 1, the temperature detection module 2, the bacteria detection module 3, the disinfection module 5, the sequencing module 6, the normal temperature storage module 7, the flora evaluation module 8 and the display module 9 and is used for controlling the normal work of each module through a host;
the disinfection module 5 is connected with the main control module 4 and is used for disinfecting the culture environment of the intestinal flora through disinfection equipment;
the sequencing module 6 is connected with the main control module 4 and is used for sequencing the intestinal flora;
the normal temperature preservation module 7 is connected with the main control module 4 and is used for preserving the intestinal flora at normal temperature through preservation liquid;
the flora evaluation module 8 is connected with the main control module 4 and used for evaluating the growth state of the intestinal flora through an evaluation program;
and the display module 9 is connected with the main control module 4 and used for displaying the acquired culture video, temperature, bacteria information and the evaluation result of the growth state of the intestinal flora through a display.
The sequencing module 6 provided by the invention has the following sequencing method:
(1) constructing one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model through a database program; constructing an intestinal flora functional gene database;
(2) detecting the intestinal flora structure in the sample, and comparing the intestinal flora structure in the sample with one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model;
(3) detecting functional genes of intestinal flora in the sample, and comparing the functional genes with the functional gene database data of the flora; and generating an intestinal flora sequencing report.
The construction method of the flora age database provided by the invention comprises the following steps: acquiring a certain number of intestinal flora samples of healthy people of 0-100 years of age in each age group, testing the total flora amount, the total lactobacillus amount, the total bifidobacterium amount and the total escherichia coli amount of each sample, and constructing the flora structure related information of each age group to form the related relation between the age and the flora structure.
The construction method of the flora trend model provided by the invention comprises the following steps: obtaining a certain number of intestinal flora samples of people of different health conditions of 0-100 years of age, classifying the flora structures of the age and the health conditions into a plurality of flora trends according to the healthy trend.
The flora trends provided by the invention comprise extremely healthy trends, high healthy trends, relatively healthy trends, aging trends, sub-healthy trends, disease trends of grade 1, disease trends of grade 2 and disease trends of grade 3.
The construction method of the risk prediction trend model provided by the invention comprises the following steps: obtaining intestinal flora samples of a certain number of people with different diseases and different degrees of the same disease, typing the flora structures of the age group and the disease condition to form a correlation between the diseases and the disease degree, and classifying the disease degree into low, medium, high and high degrees, wherein the diseases comprise diabetes, colorectal cancer, ulcerative colitis, obesity and cardiovascular diseases.
The construction method of the nutrition absorption level model provided by the invention comprises the following steps: acquiring a certain number of intestinal flora samples of crowds with different food nutrient absorption levels, classifying the flora structure of each food with different nutrient absorption levels to form a correlation between the flora structure and each food nutrient absorption level, and classifying the nutrient absorption levels into low, medium, high and high degrees; the different foods comprise sugar, grains, rice, flour foods, meat, egg and milk foods and fat foods.
The component method of the antibiotic resistance model provided by the invention comprises the following steps: the method comprises the steps of obtaining intestinal flora samples of healthy people eating antibiotics for a certain time, typing flora structures to form a correlation between the flora structures and the healthy people eating the antibiotics for a certain time, and classifying antibiotic resistance into low, medium, high and high degrees.
The preparation method of the preservation solution in the normal-temperature preservation module 7 provided by the invention comprises the following steps:
1) selecting a cason preservative, disodium ethylene diamine tetraacetate, Tris-HCl buffer solution, lithium chloride, ethanol and sterile water; the kasong preservative accounts for 0.5 per mill of the total mass of the preservation solution; the molar fraction of the ethylene diamine tetraacetic acid disodium is 10 mM; the molar volume fraction of the Tris-HCl buffer solution is 10 mM; the molar volume fraction of the lithium chloride is 2 mol/L; the volume part of the ethanol is 95 percent; the volume fraction of the sterile water is 2%;
2) respectively weighing corresponding parts by mass of a cason preservative, and corresponding mole fractions of disodium ethylenediaminetetraacetate and lithium chloride in TrisHCl buffer solution, dissolving in corresponding volumes of ethanol, uniformly mixing, adding sterile water, and storing at normal temperature to obtain the preservation solution.
When the system works, firstly, the culture video data of the intestinal flora is collected by the culture video collecting module 1 through the camera; detecting the culture environment temperature data of the intestinal flora by using a temperature sensor through a temperature detection module 2; detecting the bacteria information of the culture environment of the intestinal flora by using a bacteria detector through a bacteria detection module 3; secondly, the main control module 4 sterilizes the culture environment of the intestinal flora by using a sterilization device through the sterilization module 5; sequencing the intestinal flora through a sequencing module 6; the intestinal flora is preserved at normal temperature by using a preservation solution through a normal-temperature preservation module 7; then, evaluating the growth state of the intestinal flora by using an evaluation program through a flora evaluation module 8; and finally, the display module 9 is used for displaying the acquired culture video, temperature, bacteria information and the evaluation result of the growth state of the intestinal flora.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (9)
1. A system for protecting the culture of intestinal flora, comprising:
the system comprises a culture video acquisition module, a temperature detection module, a bacteria detection module, a main control module, a disinfection module, a sequencing module, a normal-temperature storage module, a flora evaluation module and a display module;
the culture video acquisition module is connected with the main control module and is used for acquiring culture video data of the intestinal flora through the camera;
the temperature detection module is connected with the main control module and used for detecting the culture environment temperature data of the intestinal flora through a temperature sensor;
the bacteria detection module is connected with the main control module and used for detecting the bacteria information of the culture environment of the intestinal flora through the bacteria detector;
the main control module is connected with the culture video acquisition module, the temperature detection module, the bacteria detection module, the disinfection module, the sequencing module, the normal temperature storage module, the flora evaluation module and the display module and is used for controlling the normal work of each module through a host;
the disinfection module is connected with the main control module and is used for disinfecting the culture environment of the intestinal flora through disinfection equipment;
the sequencing module is connected with the main control module and used for sequencing the intestinal flora;
the normal-temperature preservation module is connected with the main control module and is used for preserving the intestinal flora at normal temperature through preservation liquid;
the flora evaluation module is connected with the main control module and used for evaluating the growth state of the intestinal flora through an evaluation program;
and the display module is connected with the main control module and used for displaying the acquired culture video, temperature, bacteria information and the evaluation result of the growth state of the intestinal flora through a display.
2. The system for culture protection of intestinal flora according to claim 1, wherein the sequencing module performs the following sequencing method:
(1) constructing one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model through a database program; constructing an intestinal flora functional gene database;
(2) detecting the intestinal flora structure in the sample, and comparing the intestinal flora structure in the sample with one or more of a flora age database, a flora trend model, a risk prediction trend model, a nutrition absorption level model and an antibiotic resistance model;
(3) detecting functional genes of intestinal flora in the sample, and comparing the functional genes with the functional gene database data of the flora; and generating an intestinal flora sequencing report.
3. The system for culturing and protecting intestinal flora according to claim 2, wherein the flora age database is constructed by: acquiring a certain number of intestinal flora samples of healthy people of 0-100 years of age in each age group, testing the total flora amount, the total lactobacillus amount, the total bifidobacterium amount and the total escherichia coli amount of each sample, and constructing the flora structure related information of each age group to form the related relation between the age and the flora structure.
4. The system for protecting and culturing intestinal flora according to claim 2, wherein the flora trend model is constructed by a method comprising: obtaining a certain number of intestinal flora samples of people of different health conditions of 0-100 years of age, classifying the flora structures of the age and the health conditions into a plurality of flora trends according to the healthy trend.
5. The system for culture protection of gut flora as claimed in claim 4, wherein said flora trend comprises an extreme health trend, a high health trend, a relative health trend, an aging trend, a sub-health trend, a disease trend of grade 1, a disease trend of grade 2, a disease trend of grade 3.
6. The system for culturing and protecting intestinal flora according to claim 2, wherein the risk prediction trend model is constructed by: obtaining intestinal flora samples of a certain number of people with different diseases and different degrees of the same disease, typing the flora structures of the age group and the disease condition to form a correlation between the diseases and the disease degree, and classifying the disease degree into low, medium, high and high degrees, wherein the diseases comprise diabetes, colorectal cancer, ulcerative colitis, obesity and cardiovascular diseases.
7. The system for culturing and protecting intestinal flora according to claim 2, wherein the nutrient absorption level model is constructed by: acquiring a certain number of intestinal flora samples of crowds with different food nutrient absorption levels, classifying the flora structure of each food with different nutrient absorption levels to form a correlation between the flora structure and each food nutrient absorption level, and classifying the nutrient absorption levels into low, medium, high and high degrees; the different foods comprise sugar, grains, rice, flour foods, meat, egg and milk foods and fat foods.
8. The system for culture protection of the intestinal flora according to claim 2, wherein the antibiotic resistance model comprises the following components: the method comprises the steps of obtaining intestinal flora samples of healthy people eating antibiotics for a certain time, typing flora structures to form a correlation between the flora structures and the healthy people eating the antibiotics for a certain time, and classifying antibiotic resistance into low, medium, high and high degrees.
9. The system for culturing and protecting intestinal flora according to claim 1, wherein the preparation method of the preservation solution in the ambient temperature preservation module comprises the following steps:
1) selecting a cason preservative, disodium ethylene diamine tetraacetate, Tris-HCl buffer solution, lithium chloride, ethanol and sterile water; the kasong preservative accounts for 0.5 per mill of the total mass of the preservation solution; the molar fraction of the ethylene diamine tetraacetic acid disodium is 10 mM; the molar volume fraction of the Tris-HCl buffer solution is 10 mM; the molar volume fraction of the lithium chloride is 2 mol/L; the volume part of the ethanol is 95 percent; the volume fraction of the sterile water is 2%;
2) respectively weighing corresponding parts by mass of a cason preservative, and corresponding mole fractions of disodium ethylenediaminetetraacetate and lithium chloride in TrisHCl buffer solution, dissolving in corresponding volumes of ethanol, uniformly mixing, adding sterile water, and storing at normal temperature to obtain the preservation solution.
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| CN111631758A (en) * | 2020-06-09 | 2020-09-08 | 温州芳植生物科技有限公司 | Structure and method for preserving sampling material in gastrointestinal tract |
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| CN110196251A (en) * | 2019-06-13 | 2019-09-03 | 六盘水市食品药品检验检测所 | A kind of sampling system and method for food inspection |
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| CN111631758A (en) * | 2020-06-09 | 2020-09-08 | 温州芳植生物科技有限公司 | Structure and method for preserving sampling material in gastrointestinal tract |
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