CN111135332A - Method and system for testing disinfection effect of disinfectant - Google Patents

Method and system for testing disinfection effect of disinfectant Download PDF

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CN111135332A
CN111135332A CN201911337893.8A CN201911337893A CN111135332A CN 111135332 A CN111135332 A CN 111135332A CN 201911337893 A CN201911337893 A CN 201911337893A CN 111135332 A CN111135332 A CN 111135332A
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signal
voltage
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electrode
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余江华
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Zhejiang Jiye Biotechnology Co Ltd
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Zhejiang Jiye Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26Accessories or devices or components used for biocidal treatment
    • A61L2/28Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18Liquid substances or solutions comprising solids or dissolved gases
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/22Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
    • G01N27/221Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance by investigating the dielectric properties
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/22Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
    • G01N27/226Construction of measuring vessels; Electrodes therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/22Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating capacitance
    • G01N27/228Circuits therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28Electrolytic cell components
    • G01N27/30Electrodes, e.g. test electrodes; Half-cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10Apparatus features
    • A61L2202/14Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs

Abstract

The invention discloses a method and a system for testing the disinfection effect of a disinfectant, wherein the system comprises the following components: the device comprises a power supply, a first electrode, a second electrode, a voltage sensor and a main control device; the power supply is respectively connected with the first electrode and the voltage sensor; the first electrode and the second electrode are arranged in a first disinfectant solution and are used for electrolyzing the first disinfectant solution to generate hypochlorous acid; the input end of the voltage sensor is connected with the second electrode, and the voltage sensor is used for acquiring a first voltage signal in direct proportion to the hypochlorous acid content; the main control device is connected with the output end of the voltage sensor and used for receiving the first voltage signal, comparing and analyzing the first voltage signal with preset voltage parameters and obtaining an analysis result according to the comparison, so that the problems of low precision and long test time of a disinfectant disinfection effect test are solved.

Description

Method and system for testing disinfection effect of disinfectant
Technical Field
The application relates to the technical field of disinfectant monitoring, in particular to a method and a system for testing the disinfection effect of a disinfectant.
Background
The test of the disinfection effect of the disinfectant is an important link of disinfection work. The existence of the living microorganisms in the disinfected sample or the quantity of the microorganisms contained in the disinfected sample is qualitatively or quantitatively detected through various detection technologies to detect the physicochemical factors, so that the capability of killing the microorganisms by a certain factor is obtained, and the method is the most objective means for testing the disinfection effect. In addition, the detection of the disinfection effect by biological methods is not the only technique, and it is necessary to rationalize the performance test.
In the related technology, the test method for the disinfection effect of the disinfectant is to transfer a sample after disinfection treatment to a culture medium for culture, observe whether bacteria grow or count live bacteria, and evaluate the disinfection effect by qualitative or quantitative data. For example, the activity of microorganisms alone is used to evaluate the disinfection effect of a disinfectant, and the method is tedious and does not have the effect of rapid detection; or, the sterilization degree is indicated by using a pressure steam sterilization chemical indicator, the method is simple and practical, but the testing efficiency is not high, meanwhile, the indicator needs to act for a certain time under saturated steam at a certain temperature, whether the sterilization requirement is met or not is judged through the color change of the indicator indicating color block, and the requirement on the testing environment is higher; or, the sterilization degree is indicated by using an ethylene oxide sterilization chemical indicator card, the indicator card is clamped in an object to be sterilized and is subjected to ethylene oxide sterilization treatment, and the sterilization effect can be indirectly inferred by observing the color change condition of the indicator card after sterilization, but the test precision is not high. Therefore, such methods in the related art are time-consuming and labor-consuming, thereby affecting the development of subsequent work.
Aiming at the problems of lower precision and longer test time of the disinfection effect test of the disinfectant in the related art, no effective solution is provided at present.
Disclosure of Invention
The invention provides a method and a system for testing the disinfection effect of a disinfectant, aiming at the problems of lower precision and longer test time of the disinfection effect test of the disinfectant in the related art and at least solving the problems.
According to an aspect of the present invention, there is provided a system for testing the disinfection effect of a disinfectant, the system comprising: the device comprises a power supply, a first electrode, a second electrode, a voltage sensor and a main control device;
the power supply is respectively connected with the first electrode and the voltage sensor;
the first electrode and the second electrode are arranged in a first disinfectant solution and are used for electrolyzing the first disinfectant solution to generate hypochlorous acid;
the input end of the voltage sensor is connected with the second electrode, and the voltage sensor is used for acquiring a first voltage signal in direct proportion to the hypochlorous acid content;
the main control device is connected with the output end of the voltage sensor and used for receiving the first voltage signal, comparing and analyzing the first voltage signal with preset voltage parameters and obtaining an analysis result according to the comparison.
In one embodiment, the system further comprises signal processing circuitry; the input end of the signal processing circuit is connected with the output end of the voltage sensor, and the signal processing circuit is used for processing and amplifying the first voltage signal and outputting a second voltage signal;
the output end of the signal processing circuit is connected with the main control device;
and the main control device receives the second voltage signal, compares and analyzes the second voltage signal and the preset voltage parameter, and acquires an analysis result according to the comparison.
In one embodiment, the signal processing circuit includes: a signal amplifying unit and a signal filtering unit;
the input end of the signal amplification unit is connected with the output end of the voltage sensor; the signal amplification unit comprises a differential operational amplifier, and is used for amplifying the received first voltage signal and outputting a noise voltage signal;
the input end of the signal filtering unit is connected with the output end of the signal amplifying unit, and the output end of the signal filtering unit is connected with the main control device; the signal filtering unit comprises an active fourth-order filter and is used for filtering the received noise voltage signal and outputting the second voltage signal to the main control device.
In one embodiment, the system further comprises a PH sensor;
the PH sensor is arranged in the first disinfectant solution and is connected with the main control device; the PH sensor sends the acquired PH detection signal to the master control device;
and the main control device compares and analyzes the PH detection signal with a preset PH parameter and obtains an analysis result according to the comparison.
In one embodiment, the system further comprises a biosensor;
the biosensor is arranged in a second disinfectant solution and is connected with the main control device; the biological sensor sends the collected microorganism detection signal to the main control device; the second disinfectant solution contains microorganisms, and the disinfectant content of the second disinfectant solution is equal to that of the first disinfectant solution;
and the master control device compares and analyzes the microorganism detection signal with a preset microorganism parameter, and obtains an analysis result according to the comparison.
In one embodiment, the system further comprises an alarm;
and the alarm receives a first trigger signal sent by the main control device and sends alarm information according to the first trigger signal under the condition that the analysis result is that the test fails.
In one embodiment, the system further comprises a display device; and the display device receives and displays the analysis result sent by the main control device.
According to another aspect of the present invention, there is provided a method for testing the disinfection effect of a disinfection solution, the method comprising:
receiving a first voltage signal which is sent by a voltage sensor and is in direct proportion to the hypochlorous acid content generated by the electrolysis of the first disinfectant solution; wherein, the power supply is respectively connected with the first electrode and the voltage sensor; the first electrode and the second electrode are arranged in the first disinfectant solution, and the voltage sensor is connected with the second electrode;
and comparing and analyzing the first voltage signal with a preset voltage parameter, and acquiring an analysis result according to the comparison.
In one embodiment, the obtaining the analysis result according to the comparison includes:
receiving a PH detection signal sent by a PH sensor; wherein the pH sensor is disposed in the first disinfectant solution;
and carrying out contrastive analysis on the PH detection signal and a preset PH parameter, carrying out contrastive analysis on the first voltage signal and the preset voltage parameter, and obtaining an analysis result according to the contrastive analysis.
According to another aspect of the present invention, there is provided a computer device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, the processor implementing the steps of any of the methods described above when executing the computer program.
By the invention, a method and a system for testing the disinfection effect of a disinfectant are adopted, and the system comprises the following steps: the device comprises a power supply, a first electrode, a second electrode, a voltage sensor and a main control device; the power supply is respectively connected with the first electrode and the voltage sensor; the first electrode and the second electrode are arranged in a first disinfectant solution and are used for electrolyzing the first disinfectant solution to generate hypochlorous acid; the input end of the voltage sensor is connected with the second electrode, and the voltage sensor is used for acquiring a first voltage signal in direct proportion to the hypochlorous acid content; the main control device is connected with the output end of the voltage sensor and used for receiving the first voltage signal, comparing and analyzing the first voltage signal with preset voltage parameters and obtaining an analysis result according to the comparison, so that the problems of low precision and long test time of a disinfectant disinfection effect test are solved.
Drawings
FIG. 1 is a diagram illustrating an application scenario of a test system according to an embodiment of the present invention;
FIG. 2 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
FIG. 3 is a block diagram of a second embodiment of a system for testing the disinfection effect of a disinfection solution according to the present invention;
FIG. 4 is a circuit schematic of a signal processing circuit according to an embodiment of the present invention;
FIG. 5 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
fig. 6 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
fig. 7 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
FIG. 8 is a first flowchart of a method for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
FIG. 9 is a flowchart illustrating a method for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention;
fig. 10 is a flow chart of a method for testing the disinfection effect of the disinfectant according to the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.
In this embodiment, an application scenario of a test system for a disinfection effect of a disinfection solution is provided, fig. 1 is a schematic view of an application scenario of a test system according to an embodiment of the present invention, as shown in fig. 1, a 1L of aqueous solution is placed in a test container, a quantitative disinfection solution is added into the test container, a first electrode 14 and a second electrode 16 are inserted into the test container, a PH sensor is disposed at the bottom of the test container, and the first electrode 14, a power supply 12, a voltage sensor 18, and the second electrode 16 are sequentially connected through a lead; the voltage sensor 18 is used for collecting a voltage signal which is in direct proportion to the hypochlorous acid content generated by the electrolysis of the first disinfectant solution, and the PH sensor 110 is used for detecting the PH value of the solution in the test container; a container identical to the test container is additionally provided, and 1L of the aqueous solution is also placed therein, and the same disinfectant as the disinfectant added to the test container is added thereto, while a predetermined amount of microorganisms is injected into the container, and the biosensor is provided in the container for detecting the activity of the microorganisms.
In this embodiment, a system for testing the disinfection effect of a disinfection solution is provided, fig. 2 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 2, the system includes: a power source 12, a first electrode 14, a second electrode 16, a voltage sensor 18, and a master control device 22;
the power source 12 is connected to the first electrode 14 and the voltage sensor 18, respectively; the power supply 12 is used to power the entire test system.
The first electrode 14 and the second electrode 16 are disposed in a first disinfectant solution for electrolyzing the first disinfectant solution to generate hypochlorous acid; wherein the free chlorine is also called free residual chlorine, which refers to hypochlorous acid (HClO) and hypochlorite ion (ClO) in disinfectant-) And dissolved elemental chlorine (Cl)2) (ii) a The bactericidal effect of HClO is that ClO is in the same concentration-About 80 times of the total weight of the product.
The input end of the voltage sensor 18 is electrically connected with the second electrode 16, and the voltage sensor 18 is used for acquiring a first voltage signal which is in direct proportion to the hypochlorous acid content; specifically, a higher voltage value collected by the voltage sensor 18 indicates a higher hypochlorous acid content.
The main control device 22 is connected with the output end of the voltage sensor 18, and the main control device 22 comprises an integrated processing chip and an analysis module; the integrated processing chip transmits the received first voltage signal to the analysis module; the analysis module is used for comparing and analyzing the first voltage signal with a preset voltage parameter, and judging that the disinfection effect of the disinfectant is good and acquiring an analysis result of passing a test under the condition that the first voltage signal is greater than or equal to the preset voltage parameter; otherwise, the obtained analysis result is test failure; in addition, the main control device 22 may also be a single chip, and the received first voltage signal is processed and analyzed by the single chip.
In the related art, a disinfected sample needs to be subjected to long-time microbial culture to detect the disinfection effect, but in the embodiment of the invention, the first electrode 14 and the second electrode 16 are used for electrolyzing the solution containing the disinfectant, and the voltage signal acquired by the voltage sensor 18 is used for detecting the content of free chlorine in the solution, so that the high-efficiency detection of the disinfection effect of the disinfectant is realized, and the problems of low precision and long test time of the disinfection effect test of the disinfectant are solved; meanwhile, the received voltage signal is analyzed and processed through the analysis device, and the automatic acquisition of the detection result of the disinfection effect of the disinfectant is realized.
In an embodiment, a test system for a disinfection effect of a disinfection solution is provided, fig. 3 is a block diagram of a structure of a test system for a disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 3, the test system further includes a signal processing circuit 32; the input end of the signal processing circuit 32 is connected to the output end of the voltage sensor 18, the signal processing circuit 32 may be a differential amplifying circuit or a programmable amplifier, and the signal processing circuit 32 is configured to process and amplify the first voltage signal and output a second voltage signal;
the output end of the signal processing circuit 32 is connected with the main control device 22; the main control device 22 receives the second voltage signal, compares the second voltage signal with the preset voltage parameter, and obtains an analysis result according to the comparison.
Through the above embodiment, the signal processing circuit 32 is connected to the voltage sensor 18, and the signal processing circuit 32 performs targeted voltage signal processing on the weak voltage signals acquired by the voltage sensor 18 and outputs the voltage signals with higher precision to the main control device 22 for analysis, thereby improving the detection precision of the disinfection effect of the test system.
In one embodiment, the signal processing circuit 32 includes: a signal amplifying unit and a signal filtering unit;
the input end of the signal amplification unit is connected with the output end of the voltage sensor 18; the signal amplification unit comprises a differential operational amplifier, and is used for amplifying the received first voltage signal and outputting a noise voltage signal;
the input end of the signal filtering unit is connected with the output end of the signal amplifying unit, and the output end of the signal filtering unit is connected with the main control device 22; the signal filtering unit includes an active fourth-order filter, and is configured to filter the received noise voltage signal and output the second voltage signal to the main control device 22; the present embodiment may also configure the signal filtering unit to other orders in consideration of filtering effect.
Fig. 4 is a schematic circuit diagram of a signal processing circuit according to an embodiment of the present invention, as shown in fig. 4. The signal amplifying unit includes a first resistor R1, a second resistor R2, a third resistor R3, a first operational amplifier a1, and a second operational amplifier a 2.
The output end of the voltage sensor 18 is connected with the non-inverting input end of A1, the non-inverting end of A2 is grounded, the output end of A2 is connected with one end of R3, the inverting input end of A2 is connected with the other end of R3, the other end of R3 is connected with one end of R2, the other end of R2 is connected with the inverting input end of A1, the other end of R2 is connected with one end of R1, the other end of the resistor R1 is connected with the output end of A1, and the other end of R1 is connected with the input end of the signal filtering unit.
Specifically, the signal amplifying unit is a differential operational amplifier formed by two non-inverting operational amplifiers connected in parallel, V0 is a first voltage signal collected by the voltage sensor 18, the first voltage signal V0 is added to the non-inverting input terminal of the first operational amplifier a1, and the differential input resistance is approximately the sum of the common-mode input resistances of the two operational amplifiers, so as to obtain a higher input resistance, and obtain a noise voltage signal V01 output by the signal amplifying unit, as shown in formula 1:
v01 ═ V0 ═ I (R1+ R2+ R3) formula 1
The closed loop gain a of the signal amplification unit is shown in equation 2:
Figure BDA0002331447660000071
the signal filtering unit comprises a fourth resistor R4, a fifth resistor R5, a sixth resistor R6, a seventh resistor R7, an eighth resistor R8, a ninth resistor R9, a tenth resistor R10, an eleventh resistor R11, a first capacitor C1, a second capacitor C2, a third capacitor C3, a fourth capacitor C4 and a third operational amplifier A3.
Wherein, the output end of the signal amplification unit is connected with one end of R4, one end of R5 is connected with one end of C2, the other end of R4 is connected with the other end of R5, the other end of R5 is further connected with one end of R6, the other end of R6 is connected with one end of C1, the other end of R6 is further connected with one end of R7, the other end of R7 is connected with one end of R8, the other end of R8 is connected with one end of R9, one end of C2 is connected with one end of C4, one end of C2 is connected with one end of R10, one end of C2 is grounded, the other end of C2 is connected with the other end of R7, one end of C1 is connected with one end of R7, one end of C7 is connected with one end of R7, the other end of C7 is connected with the other end of C7, the other end of C7 is connected with the output end of a7, the other end of R7 is connected with the other end of R7, the input end of R7 is connected with the other end of R7, the C7, the input end of the, one end of the R11 is connected with the other end of the R10, the other end of the R11 is connected with the output end of the A3, the other end of the R11 is also connected with the input end of the A3, and the signal filtering unit transmits the voltage signal V1 to the main control device 22.
Specifically, in the signal filtering unit, the resistance value of R4 is 33k Ω, the resistance value of R5 is 10k Ω, the resistance value of R6 is 90k Ω, the resistance value of R7 is 470k Ω, the resistance value of R8 is 470k Ω, the resistance value of R9 is 33k Ω, the resistance value of R10 is 10k Ω, the resistance value of R11 is 8k Ω, and the capacitance values of C1, C2, C3, and C4 are all 1 μ F.
Because the signal transmitted to the signal filtering unit by the signal amplifying circuit includes a noise signal, the signal filtering unit designed by the embodiment of the present invention filters the low-frequency random impulse noise interference voltage of the third voltage signal, and outputs the second voltage signal V1, the cutoff frequency of the signal filtering unit is 8Hz, and the transfer function is as shown in formula 3:
Figure BDA0002331447660000081
when C1 ═ C2 ═ C3 ═ C4 ═ C is substituted into formula 3, the transfer function is obtained as shown in formula 4:
Figure BDA0002331447660000082
wherein, the noise of the signal processing circuit 32 is within 5.25nV, and the drift is 1.15 muV/DEG C; the first operational amplifier a1 and the second operational amplifier a2 are both LT1192 in type, and the third operational amplifier A3 is μ a741 in type.
Through the above embodiment, the signal amplification unit in the signal processing circuit 32 amplifies the voltage signal collected by the voltage sensor 18 and outputs a noise voltage signal, and meanwhile, the signal filtering unit filters the noise signal included in the noise voltage signal, so as to output a second voltage signal with an obvious and noiseless signal to the main control device 22, thereby facilitating the main control device 22 to accurately and quickly analyze the voltage signal, and further improving the accuracy of the test system for the disinfection effect of the disinfectant.
In an embodiment, a system for testing the disinfection effect of a disinfectant is provided, fig. 5 is a block diagram of a system for testing the disinfection effect of a disinfectant according to an embodiment of the present invention, as shown in fig. 5, the system further includes a PH sensor 110;
the PH sensor 110 is disposed in the first disinfectant solution and connected to the main control device 22; the PH sensor 110 sends the collected PH detection signal to the main control device 22; wherein, in the disinfectant, the proportion of hypochlorous acid in free chlorine has a significant relation with the PH value, and when the disinfectant is produced, the PH value of the disinfectant is required to be adjusted to be in the range of 6.0 to 7.0, and the proportion of hypochlorous acid is high and the disinfection capability is strong.
The main control device 22 performs comprehensive processing and analysis on the received PH detection signal and the first/second voltage signal to obtain a disinfection effect of a disinfection solution; specifically, the main control device 22 stores a preset PH parameter and a preset voltage parameter, the main control device 22 compares the PH detection signal with the preset PH parameter and analyzes the PH detection signal, and compares the voltage signal with the preset voltage parameter, and when the PH detection signal is within the range of the preset PH parameter and the voltage signal is greater than or equal to the preset voltage parameter, the analysis module judges that the disinfection effect of the disinfectant is good, and obtains an analysis result that the test is passed; otherwise, the obtained analysis result is test failure.
Through above-mentioned embodiment, through placing PH sensor 110 in first antiseptic solution, this PH sensor 110 sends the PH detected signal who gathers for this main control unit 22, and this main control unit 22 carries out the integrated analysis to this PH detected signal and voltage signal and handles, to making more effective accurate to the analysis of this antiseptic solution disinfection effect, has further improved accuracy and the comprehensiveness of this test system to antiseptic solution disinfection effect.
In an embodiment, a system for testing the disinfection effect of a disinfection solution is provided, fig. 6 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 6, the system further includes a biosensor 62;
the biosensor 62 is disposed in the second disinfectant solution and connected to the main control device 22; the biological sensor 62 sends the collected microorganism detection signal to the main control device 22; wherein the second disinfectant solution contains microorganisms, and the disinfectant content of the second disinfectant solution is equal to that of the first disinfectant solution; the biosensor 62 includes: a molecular recognition element and a signal converter; the molecule recognition element is a bioactive substance (such as tissue, microbial cell, organelle, cell receptor, enzyme, antibody, nucleic acid, etc.) with molecule recognition capability; the signal converter can be an electrochemical electrode, an optical detection element, a gas-sensitive electrode, a thermistor, a field effect transistor, a piezoelectric crystal, a surface plasma resonance device or the like. When the disinfectant solution is specifically bound to the molecular recognition element, the generated complex is converted into an electric signal that can be output, i.e., a microorganism detection signal, by a signal converter, and the microorganism detection signal is transmitted to the main control device 22, thereby achieving the purpose of analysis and detection.
Specifically, the biosensor 62 is a piezoelectric sensor, which is mainly composed of a piezoelectric quartz crystal and a pair of parallel plate metal electrodes inserted in a solution in series, and has a sensitive frequency shift response to a change in an electrical parameter between the electrodes. The change in frequency is determined primarily by the change in the conductance and dielectric constant of the solution, when other parameters are unchanged. During the process of destroying the microorganisms contained in the second disinfectant solution, the composition of the second disinfectant solution changes, and the electrochemical properties of the solution, such as the solution conductivity, change. The piezoelectric acoustic wave sensor can sensitively detect the change and amplify and output a microorganism detection signal, so that the detection sensitivity is improved.
In particular, the biosensor 62 may also be a microbial sensor that, by integrating a biological recognition element with a physical conductor, converts into a measurement signal that varies proportionally with the microbial concentration of the second antiseptic solution.
Specifically, the biosensor 62 may also be a fluorescent microbial sensor, by first fusing a reporter gene, which is responsible for the generation of a measurable optical signal, with an inducer gene, which is activated when the target analyte is present, causing the reporter gene to be on (activated) or off (inhibited); further, the fluorescent microbial sensor is classified into two types, in vivo and in vitro. In vivo type microbial sensors refer to microorganisms that are capable of producing a fluorescent substrate (e.g., green fluorescent protein) without the addition of exogenous fluorochromes. The action principle of the in-vitro microbial sensor is as follows: the metabolism of the microorganisms changes their surroundings, resulting in a change in the light emission of the foreign fluorescent substance.
Specifically, the biosensor 62 may also be a luminescent microbial sensor capable of measuring changes in the light emitted by living microorganisms, and such changes in light emission are in effect a dose-dependent reaction of luciferase encoded by the lux gene against the target analyte.
The main control device 22 performs comprehensive processing analysis on the received microorganism detection signal, the received PH detection signal and the received first/second voltage signal; specifically, the main control device 22 stores a preset microorganism parameter, a preset PH parameter and a preset voltage parameter, and under the condition that the microorganism detection signal is greater than the preset microorganism parameter, the PH detection signal is within the range of the preset PH parameter, and the voltage signal is greater than or equal to the preset voltage parameter, the analysis module judges that the disinfection effect of the disinfectant is good, and obtains an analysis result that the test is passed; otherwise, the obtained analysis result is test failure.
Through the embodiment, the biosensor 62 is placed in the second disinfectant solution, and the biosensor 62 detects the microbial activity in the second disinfectant solution, so that auxiliary detection of a test system for the disinfection effect of the disinfectant is realized, and the accuracy of the test system is ensured.
In one embodiment, the system further comprises an alarm;
under the conditions that the first/second voltage signal received by the main control device 22 is smaller than the preset voltage parameter, the PH detection signal is not within the preset PH parameter range or the microorganism detection signal is larger than the preset microorganism parameter, the main control device 22 judges that the disinfection effect of the disinfectant is not good, the analysis result of the main control device 22 is a test failure, meanwhile, the main control device 22 sends a first trigger signal to an alarm, and the alarm sends alarm information after receiving the first trigger signal; in addition, if the first/second voltage signal received by the main control device 22 is greater than or equal to the preset voltage parameter, the PH detection signal is within the preset PH parameter range, and the microorganism detection signal is less than or equal to the preset microorganism parameter, the main control device 22 determines that the disinfection effect of the disinfectant is good, meanwhile, the main control device 22 sends a second trigger signal to the alarm, and the alarm does not send alarm information after receiving the second trigger signal.
Specifically, the first trigger signal is a high level signal, and the second trigger signal is a low level signal.
Through the embodiment, the alarm is connected with the main control device 22, receives the trigger signal sent by the main control device 22 according to the analysis result, and gives an alarm under the condition that the trigger signal indicates that the test fails, so that the tester is timely reminded that the disinfection effect of the disinfectant does not reach the standard.
In an embodiment, a system for testing the disinfection effect of a disinfection solution is provided, fig. 7 is a block diagram of a system for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 7, the system further includes a display device 72;
the display device 72 receives and displays the analysis result sent by the main control device 22; meanwhile, the display device 72 can also display the first/second voltage signal, the PH detection signal and the microorganism detection signal sent by the main control device 22, so that the tester can conveniently master the specific result of the disinfection effect test of the disinfection solution.
In this embodiment, a method for testing a disinfection effect of a disinfection solution is provided, and fig. 8 is a flowchart illustrating a method for testing a disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 8, the method includes the following steps:
step S802, receiving a first voltage signal which is sent by the voltage sensor 18 and is in direct proportion to the hypochlorous acid content generated by the electrolysis of the first disinfectant solution; wherein the power source 12 is connected to the first electrode 14 and the voltage sensor 18, respectively; the first electrode 14 and the second electrode 16 are placed in the first disinfectant solution, and the voltage sensor 18 is connected to the second electrode 16;
step S804, comparing and analyzing the first voltage signal with a preset voltage parameter, and obtaining an analysis result according to the comparison.
Through the steps S802 to S804, the first voltage signal which is sent by the voltage sensor 18 and is in direct proportion to the hypochlorous acid content generated by the electrolysis of the first disinfectant solution is received, and the first voltage signal is contrasted and analyzed, so that an analysis result is obtained, the efficient detection of the disinfection effect of the disinfectant is realized, and the problems of low precision and long test time of the disinfection effect test of the disinfectant are solved.
In an embodiment, a method for testing the disinfection effect of a disinfection solution is provided, and fig. 9 is a flowchart illustrating a method for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 9, the method includes the following steps:
step S902, receiving a PH detection signal sent by the PH sensor 110; wherein, the PH sensor 110 is arranged in the first disinfectant solution;
step S904, comparing and analyzing the PH detection signal with a preset PH parameter, comparing and analyzing the first voltage signal with the preset voltage parameter, and obtaining an analysis result according to the comparison.
In an embodiment, a method for testing the disinfection effect of a disinfection solution is provided, and fig. 10 is a flow chart of a method for testing the disinfection effect of a disinfection solution according to an embodiment of the present invention, as shown in fig. 10, the method includes the following steps:
step S1002, receiving a microorganism detection signal transmitted by the biosensor 62; wherein the biosensor 62 is placed in a second antiseptic solution; the second disinfectant solution contains microorganisms, and the disinfectant content of the second disinfectant solution is equal to that of the first disinfectant solution;
step S1004, comparing and analyzing the microorganism detection signal with a preset microorganism parameter, comparing and analyzing the first voltage signal with the preset voltage parameter, and obtaining an analysis result according to the comparison.
It should be understood that, although the respective steps in the flowcharts of fig. 8 to 10 are sequentially shown as indicated by arrows, the steps are not necessarily sequentially performed in the order indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least some of the steps in fig. 8-10 may include multiple sub-steps or multiple stages that are not necessarily performed at the same time, but may be performed at different times, and the order of performing the sub-steps or stages is not necessarily sequential, but may be performed in turn or alternately with other steps or at least some of the sub-steps or stages of other steps.
In one embodiment, a computer device is provided, which may be a server. The computer device includes a processor, a memory, a network interface, and a database connected by a system bus. Wherein the processor of the computer device is configured to provide computing and control capabilities. The memory of the computer device comprises a nonvolatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for the operation of an operating system and computer programs in the non-volatile storage medium. The database of the computer device is used for storing analysis result data. The network interface of the computer device is used for communicating with an external terminal through a network connection. The computer program is executed by the processor to realize a method for testing the disinfection effect of the disinfectant.
In one embodiment, a computer device is provided, which includes a memory, a processor, and a computer program stored in the memory and executable on the processor, and the processor executes the computer program to implement the steps of the disinfection effect test method of disinfectant provided in the above embodiments.
It will be understood by those skilled in the art that all or part of the processes of the methods of the embodiments described above can be implemented by hardware instructions of a computer program, which can be stored in a non-volatile computer-readable storage medium, and when executed, can include the processes of the embodiments of the methods described above. Any reference to memory, storage, database, or other medium used in the embodiments provided herein may include non-volatile and/or volatile memory, among others. Non-volatile memory can include read-only memory (ROM), Programmable ROM (PROM), Electrically Programmable ROM (EPROM), Electrically Erasable Programmable ROM (EEPROM), or flash memory. Volatile memory can include Random Access Memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in a variety of forms such as Static RAM (SRAM), Dynamic RAM (DRAM), Synchronous DRAM (SDRAM), Double Data Rate SDRAM (DDRSDRAM), Enhanced SDRAM (ESDRAM), Synchronous Link DRAM (SLDRAM), Rambus Direct RAM (RDRAM), direct bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM).
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A system for testing the disinfection effect of a disinfection fluid, the system comprising: the device comprises a power supply, a first electrode, a second electrode, a voltage sensor and a main control device;
the power supply is respectively connected with the first electrode and the voltage sensor;
the first electrode and the second electrode are arranged in a first disinfectant solution and are used for electrolyzing the first disinfectant solution to generate hypochlorous acid;
the input end of the voltage sensor is connected with the second electrode, and the voltage sensor is used for acquiring a first voltage signal in direct proportion to the hypochlorous acid content;
the main control device is connected with the output end of the voltage sensor and used for receiving the first voltage signal, comparing and analyzing the first voltage signal with preset voltage parameters and obtaining an analysis result according to the comparison.
2. The test system of claim 1, wherein the system further comprises signal processing circuitry; the input end of the signal processing circuit is connected with the output end of the voltage sensor, and the signal processing circuit is used for processing and amplifying the first voltage signal and outputting a second voltage signal;
the output end of the signal processing circuit is connected with the main control device;
and the main control device receives the second voltage signal, compares and analyzes the second voltage signal and the preset voltage parameter, and acquires an analysis result according to the comparison.
3. The test system of claim 2, wherein the signal processing circuit comprises: a signal amplifying unit and a signal filtering unit;
the input end of the signal amplification unit is connected with the output end of the voltage sensor; the signal amplification unit comprises a differential operational amplifier, and is used for amplifying the received first voltage signal and outputting a noise voltage signal;
the input end of the signal filtering unit is connected with the output end of the signal amplifying unit, and the output end of the signal filtering unit is connected with the main control device; the signal filtering unit comprises an active fourth-order filter and is used for filtering the received noise voltage signal and outputting the second voltage signal to the main control device.
4. The test system of claim 1, wherein the system further comprises a PH sensor;
the PH sensor is arranged in the first disinfectant solution and is connected with the main control device; the PH sensor sends the acquired PH detection signal to the master control device;
and the main control device compares and analyzes the PH detection signal with a preset PH parameter and obtains an analysis result according to the comparison.
5. The test system of claim 1, wherein the system further comprises a biosensor;
the biosensor is arranged in a second disinfectant solution and is connected with the main control device; the biological sensor sends the collected microorganism detection signal to the main control device; the second disinfectant solution contains microorganisms, and the disinfectant content of the second disinfectant solution is equal to that of the first disinfectant solution;
and the master control device compares and analyzes the microorganism detection signal with a preset microorganism parameter, and obtains an analysis result according to the comparison.
6. The test system of claim 1, wherein the system further comprises an alarm;
and the alarm receives a first trigger signal sent by the main control device and sends alarm information according to the first trigger signal under the condition that the analysis result is that the test fails.
7. The test system according to any one of claims 1 to 6, wherein the system further comprises a display device; and the display device receives and displays the analysis result sent by the main control device.
8. A method for testing the disinfection effect of a disinfection solution, which is characterized by comprising the following steps:
receiving a first voltage signal which is sent by a voltage sensor and is in direct proportion to the hypochlorous acid content generated by the electrolysis of the first disinfectant solution; wherein, the power supply is respectively connected with the first electrode and the voltage sensor; the first electrode and the second electrode are arranged in the first disinfectant solution, and the voltage sensor is connected with the second electrode;
and comparing and analyzing the first voltage signal with a preset voltage parameter, and acquiring an analysis result according to the comparison.
9. The method of claim 8, wherein said obtaining analysis results from said comparison comprises:
receiving a PH detection signal sent by a PH sensor; wherein the pH sensor is disposed in the first disinfectant solution;
and carrying out contrastive analysis on the PH detection signal and a preset PH parameter, carrying out contrastive analysis on the first voltage signal and the preset voltage parameter, and obtaining an analysis result according to the contrastive analysis.
10. A computer arrangement comprising a memory, a processor and a computer program stored on the memory and executable on the processor, characterized in that the steps of the method as claimed in claim 8 or 9 are implemented by the processor when executing the computer program.
CN201911337893.8A 2019-12-23 2019-12-23 Method and system for testing disinfection effect of disinfectant Pending CN111135332A (en)

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