CN111123498A - Collecting lens for living cell observation system - Google Patents
Collecting lens for living cell observation system Download PDFInfo
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- CN111123498A CN111123498A CN201911375176.4A CN201911375176A CN111123498A CN 111123498 A CN111123498 A CN 111123498A CN 201911375176 A CN201911375176 A CN 201911375176A CN 111123498 A CN111123498 A CN 111123498A
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- lens
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- single lens
- double
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/14—Condensers affording illumination for phase-contrast observation
Abstract
The invention discloses a condenser lens for a living body cell observation system, which consists of a first component and a second component which are coaxially arranged from a light source side to a sample side in sequence; the first component and the second component form a retrofocus optical system; the first component is a meniscus double-cemented lens with negative focal power; the second component consists of a first single lens and a second single lens which are coaxially arranged in sequence and has positive focal power; the meniscus double-cemented lens is formed by sequentially and coaxially arranging a double-concave single lens with negative focal power and a double-convex single lens with positive focal power, wherein the concave surface faces the light source; the first single lens is a biconvex lens with positive focal power; the second single lens is a plano-convex lens with positive focal power, and the plane faces to the sample side; the invention realizes achromatic design in visible light wave band, provides high NA and has longer working distance than focal length, the system structure is simple and compact, and the invention is especially suitable for living body cell observation system with longer working distance.
Description
Technical Field
The invention belongs to the field of microscopic imaging, and particularly relates to a condenser lens for a living cell observation system.
Background
In order to meet the demands for cell culture in the fields of vaccines, monoclonal antibodies, cell therapy, and the like, it is necessary to observe the growth, morphology, and the like of cells in culture. Ordinary bright field microscopes can only view colored or stained sections, but the stain will "kill" the cells to be viewed. Zernike invented the phase contrast imaging technique according to the fluctuation theory of light and the abbe principle. The phase contrast imaging technique can convert a phase difference, which cannot be observed by human eyes, into an amplitude difference because living transparent cells in culture can be observed. Cell cultures are typically placed in a container (e.g., a culture plate, a petri dish, a culture flask, etc.) of a certain thickness, which requires a certain working distance of the condenser lens in the living cell observation system to ensure a sufficient working space. Increasing the working distance typically employs optical systems with long focal lengths, which results in a large overall dimension of the living cell observation system.
Disclosure of Invention
The invention aims to provide a condenser lens for a living cell observation system, which is used for the living cell observation system and can increase the working distance under the condition of keeping the focal length constant.
The technical solution for realizing the purpose of the invention is as follows:
a condenser lens for a living body cell observation system is composed of a first component and a second component which are coaxially arranged in sequence from a light source side to a sample side; the first component and the second component form a retrofocus optical system;
the first component is a meniscus double-cemented lens with negative focal power;
the second component consists of a first single lens and a second single lens which are coaxially arranged in sequence and has positive focal power;
the meniscus double-cemented lens is formed by sequentially and coaxially arranging a double-concave single lens with negative focal power and a double-convex single lens with positive focal power, wherein the concave surface faces the light source;
the first single lens is a biconvex lens with positive focal power;
the second single lens is a plano-convex lens with positive optical power, and the plane faces the sample side.
Compared with the prior art, the invention has the following remarkable advantages:
(1) the invention adopts the structure of the reverse long-distance optical system, so that the condenser lens has a working distance longer than the focal length under the condition of keeping the focal length constant, namely the focal length is shorter than the working distance, and the structure of the illumination optical system and the whole living cell observation system can be more compact.
(2) All optical lens units forming the condenser are spherical lenses, so that the manufacturing cost is low.
Drawings
Fig. 1 is a schematic diagram of a phase contrast imaging optical system having a long working distance condenser lens according to the related art.
Fig. 2 is a schematic structural diagram of the annular diaphragm.
Fig. 3 is a schematic structural diagram of the phase plate.
Fig. 4 is a schematic view of an optical structure of a condenser lens according to an embodiment of the present invention.
Detailed Description
The invention is further described with reference to the following figures and embodiments.
With reference to fig. 1-3, Phase Contrast imaging (Phase Contrast) can be used for visualizing living cells in a living cell observation system. The optical principle of phase contrast imaging is shown in fig. 1. The light emitted from the light source 1 is collimated by the collimating lens 2 and focused by the collecting lens 3 onto the ring-shaped diaphragm 4 as shown in fig. 2. The plane where the annular diaphragm 4 is located is the front focal plane of the condenser lens 5. The illumination light passes through the annular diaphragm 4 to form annular illumination light. The illumination light emitted from the condenser lens by the annular illumination light is a bundle of parallel light obtained by converging all the light emitting points on the light emitting surface of the light source 1 from all directions, and penetrates through the sample to sufficiently and uniformly illuminate the sample 6. The annular diaphragm 4 passes through the condenser lens 5 and the phase contrast objective lens 7, and then is imaged on an image space focal plane of the phase contrast objective lens. The phase contrast objective 7 is provided with a dedicated phase plate 8 (normally built-in) as shown in fig. 3 on the image focal plane, so that the annular diaphragm 4 is conjugate to the phase plate 8. The background light collected by the phase contrast objective lens 7 and the scattered light scattered by the sample 6 are separated by the phase plate 8 at the back focal plane thereof, and are focused on the imaging plane of the camera 10 through the tube lens 9 to form a final phase contrast image, enabling visual observation of the living cells.
Wherein, the distance between the condenser 5 and the sample 6 is the working distance WD of the condenser 5. On the one hand, the cells are usually located in a culture vessel of a certain thickness, which requires a certain working distance of the condenser 5 to ensure a sufficient working space. On the other hand, the condenser 5 not only provides uniform illumination over the entire field of view, but also projects an annular diaphragm onto a dedicated phase plate on the image-wise focal plane of the phase contrast objective with the phase contrast objective constituting an optical system, i.e. the annular diaphragm 4 has an imaging conjugate relationship with the phase plate 8. Diameter D of the annular diaphragm 4ringFocal length F of the condenser 5conFocal length F of the phase contrast objective 7objAnd diameter D of the phase plate 8phThe following relationship is satisfied:
the long focal length condenser is generally selected for increasing the working distance WD, which increases the diameter of the annular diaphragm, resulting in a large size of the illumination optical system and the whole living cell observation system, and thus a long working distance needs to be obtained while keeping the focal length constant.
Referring to fig. 4, a condenser lens for use in an observation system of living cells of the present invention is composed of a first component G1 and a second component G2 coaxially disposed in this order from a light source side to a sample side; the first element G1 and the second element G2 constitute a retrofocus optical system;
the first component G1 is a meniscus double cemented lens L1, and has negative focal power;
the second component G2 is composed of a first single lens L2 and a second single lens L3 which are coaxially arranged in sequence and has positive focal power;
the meniscus double cemented lens L1 is formed by sequentially and coaxially cementing a double-concave single lens L101 with negative focal power and a double-convex single lens L102 with positive focal power, and the concave surface of the meniscus double cemented lens L1 faces the light source.
The first single lens L2 is a double convex lens having a positive optical power;
the second single lens L3 is a plano-convex lens with positive optical power, and the plane faces the sample side.
The distance between the plane of the second single lens L3 and the sample and the focal length of the whole condenser satisfy WD/FconWhere WD is the distance between the plane of the second einzel lens L3 and the sample, i.e. the working distance of the condenser, FconIs the focal length of the collection optic.
In a further embodiment, the meniscus doublet L1 satisfies | ν1-ν2L > 25, where v1Is the Abbe number, v, of the biconcave single lens L1012For the abbe number of the biconvex single lens L102, chromatic aberration generated when the field stop provided in the illumination optical system is imaged on the sample surface can be corrected well, so that the optimal focus position of the field stop can be known and adjusted easily.
In a further embodiment, the focal length of the second single lens L3 and the focal length of the entire condenser lens satisfy 1 < fL3/Fcon< 2.5, wherein fL3Is the focal length of the second single lens L3. Since the meniscus double cemented lens L1 of G1 in the first component has a negative power, the spherical aberration of G2 generated by the second component composed of a plurality of positive power single lenses can be eliminated by the negative power of the first component G1. Therefore, the annular diaphragm can be accurately projected onto the phase plate on the back focal plane of the phase contrast objective lens, and a phase contrast image with good contrast can be obtained. When f isL3/FconAt 1, the refractive power of the second single lens L3 is too strong, and the degree of deflection of light is higher, so that the spherical aberration generated by the second single lens L3 cannot be corrected well by the meniscus double cemented lens in the first element G1. When f isL3/FconAt ≧ 2.5, the refractive power of the second single lens L3 becomes weak, and the refractive power of the first single lens L2 becomes strong, resulting in failure to correct the spherical aberration generated by the first single lens L2 by the meniscus type doublet lens in the first component G1.
Fig. 4 is a schematic structural diagram of a condenser lens in a preferred embodiment of the present invention. Specific optical parameters are shown in the following table, where numbers s1 to s10 are the numbers of the respective optical surfaces of the condenser lens from the light source side to the sample side.
The reference number s9 in the table is an aqueous solution of the sample. Since in the living cell observation system, living cells are usually observed in a culture solution, a partial region between the sample 6 and the condenser lens is covered with water.
Focal length F of condensercon36.43mm, the distance between the plane of the second single lens L3 and the sample, i.e., the working distance WD of the condenser, is 48.98mm, and the WD/F is satisfiedcon1.34 > 1, a longer working distance than the focal length can be achieved.
In the meniscus double cemented lens L1 in the first element G1, the Abbe number v of the double concave single lens L1011Abbe number v of biconvex single lens L1022Satisfy | v1-ν228.7 > 25, so that the field stop in the illumination optical system is imaged on the sample surface without generating chromatic aberration, and the focus position of the field stop image can be easily adjusted.
Focal length f of second single lens L3L3Focal length F from the condenser 5conRatio f ofL3/Fcon2.1, satisfies 1 < fL3/Fcon< 2.5, the spherical aberration of the condenser lens 5 can be corrected well.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions are also considered to be within the scope of the present invention.
Those skilled in the art will recognize that the invention may be practiced without these specific details.
Claims (4)
1. A condenser lens for use in a living cell observation system, characterized in that: composed of a first component (G1) and a second component (G2) coaxially arranged in sequence from the light source side to the sample side; the first component (G1) and the second component (G2) form a retrofocus optical system;
the first component (G1) is a meniscus double cemented lens (L1) with negative power;
the second component (G2) is composed of a first single lens (L2) and a second single lens (L3) which are coaxially arranged in sequence and have positive focal power;
the meniscus double cemented lens (L1) is formed by sequentially and coaxially gluing a double-concave single lens (L101) with negative focal power and a double-convex single lens (L102) with positive focal power, and the concave surface faces the light source;
the first single lens (L2) is a double convex lens with positive focal power;
the second single lens (L3) is a plano-convex lens with positive optical power, and the plane faces the sample side.
2. A condenser lens for use in a living cell observation system according to claim 1, wherein: satisfy WD/FconWhere WD is the distance of the plane of the second einzel lens (L3) from the sample, i.e. the working distance of the condenser, FconIs the focal length of the collection optic.
3. A condenser lens for use in a living cell observation system according to claim 1, wherein: the meniscus double cemented lens (L1) satisfies | ν1-ν2L > 25, where v1Is the Abbe number, v, of the biconcave single lens (L101)2Is the abbe number of the biconvex single lens (L102).
4. A condenser lens for use in a living cell observation system according to claim 1, wherein: satisfies 1 < fL3/Fcon< 2.5, wherein fL3Is the focal length, F, of the second singlet lens (L3)conIs the focal length of the collection optic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911375176.4A CN111123498A (en) | 2019-12-27 | 2019-12-27 | Collecting lens for living cell observation system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911375176.4A CN111123498A (en) | 2019-12-27 | 2019-12-27 | Collecting lens for living cell observation system |
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| CN111123498A true CN111123498A (en) | 2020-05-08 |
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| CN201911375176.4A Pending CN111123498A (en) | 2019-12-27 | 2019-12-27 | Collecting lens for living cell observation system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114911045A (en) * | 2022-05-31 | 2022-08-16 | 苏州秉理科技有限公司 | Microscope optical imaging system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084285A (en) * | 1992-09-18 | 1994-03-23 | 莱卡显微及系统有限公司 | Be used for microscopical reflecting light illuminating system |
| CN1123058A (en) * | 1994-02-11 | 1996-05-22 | 莱卡显微及系统有限公司 | Condenser system for a microscope |
| EP0841584A2 (en) * | 1996-11-11 | 1998-05-13 | Nikon Corporation | Condenser lens system and microscope illuminating optical system |
| JP2003156691A (en) * | 2001-11-21 | 2003-05-30 | Olympus Optical Co Ltd | Condenser lens for microscope |
| WO2006104184A1 (en) * | 2005-03-29 | 2006-10-05 | National University Corporation, Hamamatsu University School Of Medicine | Dlp type slit scanning microscope |
-
2019
- 2019-12-27 CN CN201911375176.4A patent/CN111123498A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1084285A (en) * | 1992-09-18 | 1994-03-23 | 莱卡显微及系统有限公司 | Be used for microscopical reflecting light illuminating system |
| CN1123058A (en) * | 1994-02-11 | 1996-05-22 | 莱卡显微及系统有限公司 | Condenser system for a microscope |
| EP0841584A2 (en) * | 1996-11-11 | 1998-05-13 | Nikon Corporation | Condenser lens system and microscope illuminating optical system |
| JP2003156691A (en) * | 2001-11-21 | 2003-05-30 | Olympus Optical Co Ltd | Condenser lens for microscope |
| WO2006104184A1 (en) * | 2005-03-29 | 2006-10-05 | National University Corporation, Hamamatsu University School Of Medicine | Dlp type slit scanning microscope |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114911045A (en) * | 2022-05-31 | 2022-08-16 | 苏州秉理科技有限公司 | Microscope optical imaging system |
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Application publication date: 20200508 |