CN111122882A - Total iron detection kit and preparation method thereof - Google Patents
Total iron detection kit and preparation method thereof Download PDFInfo
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- CN111122882A CN111122882A CN202010002556.XA CN202010002556A CN111122882A CN 111122882 A CN111122882 A CN 111122882A CN 202010002556 A CN202010002556 A CN 202010002556A CN 111122882 A CN111122882 A CN 111122882A
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- detection kit
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 238000001514 detection method Methods 0.000 title claims abstract description 46
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 31
- 239000000243 solution Substances 0.000 claims abstract description 105
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 70
- 239000007983 Tris buffer Substances 0.000 claims abstract description 29
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 29
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 26
- 238000002156 mixing Methods 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 13
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 13
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 13
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 239000008213 purified water Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- BCHZICNRHXRCHY-UHFFFAOYSA-N 2h-oxazine Chemical compound N1OC=CC=C1 BCHZICNRHXRCHY-UHFFFAOYSA-N 0.000 claims abstract description 11
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 11
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 6
- 235000010489 acacia gum Nutrition 0.000 claims abstract description 6
- 239000001785 acacia senegal l. willd gum Substances 0.000 claims abstract description 6
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 6
- 239000000839 emulsion Substances 0.000 claims abstract description 6
- 239000011780 sodium chloride Substances 0.000 claims abstract description 6
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims abstract description 6
- 239000004816 latex Substances 0.000 claims description 32
- 229920000126 latex Polymers 0.000 claims description 32
- 239000007987 MES buffer Substances 0.000 claims description 16
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- 239000006228 supernatant Substances 0.000 claims description 10
- BOXSCYUXSBYGRD-UHFFFAOYSA-N cyclopenta-1,3-diene;iron(3+) Chemical compound [Fe+3].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 BOXSCYUXSBYGRD-UHFFFAOYSA-N 0.000 claims description 9
- -1 polyethylene p-chloromethyl styrene copolymer Polymers 0.000 claims description 6
- 239000004005 microsphere Substances 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 230000001502 supplementing effect Effects 0.000 claims description 2
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract 1
- 239000012982 microporous membrane Substances 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 102000008133 Iron-Binding Proteins Human genes 0.000 description 7
- 108010035210 Iron-Binding Proteins Proteins 0.000 description 7
- 230000001376 precipitating effect Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000008575 Iron Assay Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 208000037157 Azotemia Diseases 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 208000015710 Iron-Deficiency Anemia Diseases 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 208000009852 uremia Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/90—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving iron binding capacity of blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/08—Hepato-biliairy disorders other than hepatitis
- G01N2800/085—Liver diseases, e.g. portal hypertension, fibrosis, cirrhosis, bilirubin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/22—Haematology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/34—Genitourinary disorders
- G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a total iron detection kit, which comprises a reagent R1 and a reagent R2; the components in the reagent R1 are as follows: tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum; the components in the reagent R2 are as follows: tris buffer solution, emulsion coated ferrous oxazine solution and ascorbic acid. The preparation method of the total iron detection kit comprises the following steps: step a), preparation of reagent R1: adding purified water into a preparation tank A, sequentially adding each component of a reagent R1 into the preparation tank, mixing and stirring, adjusting the pH value of the solution, and filtering by using a microporous filter membrane to obtain the product; step B), preparation of reagent R2: adding purified water into the preparation tank B, sequentially adding the components of the reagent R2 into the preparation tank B, mixing and stirring the materials, and adjusting the pH value of the solution; filtering with microporous membrane. By adopting the scheme disclosed by the invention, the detection efficiency is improved, and the cost of clinical disease detection is reduced.
Description
Technical Field
The invention relates to a preparation method of a total iron detection kit, in particular to a total iron detection kit and a preparation method thereof.
Background
In blood detection, total iron binding force detection is a conventional item of blood detection, and some clinical diseases can be judged by detecting the strength of the total iron binding force in blood.
In particular, the existing clinical research shows that diseases such as iron-deficiency anemia, cirrhosis, uremia and the like are easy to be important factors for obviously improving the binding force of total iron in blood.
Therefore, the detection of the strength of the total iron binding force in blood has important clinical diagnosis significance for preventing and diagnosing some diseases clinically.
However, the current major clinical methods for measuring total iron binding capacity still use atomic absorption, and some optical detection methods such as complexation photometry.
The detection means is backward, the detection period is long, the detection cost is high like an atomic absorption method, the cost of clinical detection is increased, and the detection efficiency is reduced.
Disclosure of Invention
The invention aims to solve the technical problem of providing a total iron detection kit and a preparation method thereof.
The invention solves the technical problems through the following technical scheme:
a total iron detection kit comprising a reagent R1 and a reagent R2;
the components and concentrations in the reagent R1 were as follows:
the components and concentrations in the reagent R2 were as follows:
tris buffer solution 35-60mmol/L
2-4% of emulsion coating ferrioxamine solution
Ascorbic acid 15-30 mmol/L;
preferably, the components and concentrations in the reagent R1 are as follows:
preferably, the components and concentrations in the reagent R1 are as follows:
preferably, the components and concentrations in the reagent R2 are as follows:
tris buffer solution 48mmol/L
Latex-coated ferrioxamine solution 3%
Ascorbic acid 28 mmol/L.
Preferably, the pH of the reagent R1 is between 2.0 and 3.5.
Preferably, the pH of the reagent R2 is between 6.5 and 7.8.
The invention also discloses a preparation method of the total iron detection kit, which comprises the following steps:
step a), preparation of reagent R1:
adding purified water into a preparation tank A, sequentially adding a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum into the preparation tank, mixing and stirring, adjusting the pH value of the solution to 3 in the mixing process, stirring the mixture until the solution is clarified, and filtering by using a microporous filter membrane to obtain a filtrate as a reagent R1;
step B), preparation of reagent R2:
adding purified water into a preparation tank B, sequentially adding a Tris buffer solution, a latex coated ferrocenium solution and ascorbic acid into the preparation tank B, mixing and stirring until the solution is clear, and adjusting the pH value of the solution to 7.5;
and filtering the mixed solution by using a microporous filter membrane to obtain filtrate which is the reagent R2.
Preferably, the preparation method of the latex-coated ferrocazine solution is as follows:
s1, putting latex microspheres with the particle sizes of 65nm and 120nm into MES buffer solution, adding EDAC solution, mixing uniformly, incubating for 1.5-2h at 37 ℃, centrifuging to remove supernatant, supplementing MES buffer solution until the volume is unchanged, incubating for 1.5-2h at 37 ℃, centrifuging again, and removing supernatant to obtain latex solution;
s2, adding the mixture of the ferrous oxazine and the polyethylene p-chloromethyl styrene copolymer to react for 1-3h on the basis of the latex solution obtained in the step S1, carrying out centrifugal precipitation, dispersing the precipitate in MES buffer solution, incubating for 1.5-2h at 37 ℃, centrifuging, dispersing and dissolving the precipitate in a Tris buffer solution which is one half of the total volume of the Tris buffer solution in a reagent R2, and sealing for 36-45h at 2-8 ℃ to obtain the latex coated ferrous oxazine solution.
Compared with the prior art, the invention has the following advantages:
the invention discloses a total iron detection kit and a preparation method thereof, and the total iron detection kit is successfully prepared by the technical scheme disclosed by the invention. The total iron detection kit disclosed by the invention is applied to detection of the total iron binding capacity in blood, not only can be used for quickly and efficiently detecting the total iron binding capacity in blood, but also the detection method disclosed by the invention is simpler, and can be used for quickly testing the total iron binding capacity in blood, so that the detection efficiency is improved, and the cost of clinical disease detection is reduced.
Detailed Description
The following examples are given for the detailed implementation and specific operation of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1 Total iron detection kit
The total iron detection kit comprises a reagent R1 and a reagent R2;
wherein, the components and the concentration in the reagent R1 are as follows:
the components and concentrations in reagent R2 were as follows:
tris buffer solution 48mmol/L
Latex-coated ferrioxamine solution 3%
Ascorbic acid 28 mmol/L.
Example 2 Total iron detection kit
The total iron detection kit comprises a reagent R1 and a reagent R2;
wherein, the components and the concentration in the reagent R1 are as follows:
the components and concentrations in reagent R2 were as follows:
tris buffer solution 60mmol/L
Latex-coated ferrioxamine solution 4%
Ascorbic acid 15 mmol/L.
Example 3 Total iron detection kit
The total iron detection kit comprises a reagent R1 and a reagent R2;
wherein, the components and the concentration in the reagent R1 are as follows:
the components and concentrations in the reagent R2 were as follows:
tris buffer solution 35mmol/L
Latex-coated ferrioxamine solution 2%
Ascorbic acid 15 mmol/L.
Example 4 preparation method of Total iron detection kit
In this example, the reagent R1 and the reagent R2 in example 1 are used as raw materials for preparing a total iron detection kit by the following preparation method:
step a), preparation of reagent R1:
adding purified water into a preparation tank A, sequentially adding a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum into the preparation tank, mixing and stirring, adjusting the pH value of the solution to 3 in the mixing process, stirring the mixture until the solution is clarified, and filtering by using a microporous filter membrane to obtain a filtrate as a reagent R1;
step B), preparation of reagent R2:
adding purified water into a preparation tank B, sequentially adding a Tris buffer solution, a latex coated ferrocenium solution and ascorbic acid into the preparation tank B, mixing and stirring until the solution is clear, and adjusting the pH value of the solution to 7.5;
and filtering the mixed solution by using a microporous filter membrane to obtain filtrate which is the reagent R2.
The preparation method of the emulsion-coated ferrocenium solution comprises the following steps:
s1, putting latex microspheres with the particle sizes of 65nm and 120nm into MES buffer solution, adding EDAC solution, mixing uniformly, incubating for 2h at 37 ℃, centrifuging to remove supernatant, adding MES buffer solution, adding the MES buffer solution until the volume is unchanged, incubating for h at 37 ℃, centrifuging to remove supernatant again, and obtaining latex solution;
s2, adding the mixture of the ferrous oxazine and the polyethylene p-chloromethyl styrene copolymer to react for 3 hours on the basis of the latex solution obtained in the step S1, centrifuging and precipitating, dispersing the precipitate in MES buffer solution, incubating for 2 hours at 37 ℃, centrifuging, dispersing and dissolving the precipitate in Tris buffer solution which is one half of the total volume of the Tris buffer solution in a reagent R2, and sealing for 40 hours at 6 ℃ to obtain the latex coated ferrous oxazine solution.
Example 5 preparation of Total iron assay kit
In this example, the reagent R1 and the reagent R2 in example 2 are used as raw materials for preparing a total iron detection kit by the following preparation method:
step a), preparation of reagent R1:
adding purified water into a preparation tank A, sequentially adding a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum into the preparation tank, mixing and stirring, adjusting the pH value of the solution to 3 in the mixing process, stirring the mixture until the solution is clarified, and filtering by using a microporous filter membrane to obtain a filtrate as a reagent R1;
step B), preparation of reagent R2:
adding purified water into a preparation tank B, sequentially adding a Tris buffer solution, a latex coated ferrocenium solution and ascorbic acid into the preparation tank B, mixing and stirring until the solution is clear, and adjusting the pH value of the solution to 7.5;
and filtering the mixed solution by using a microporous filter membrane to obtain filtrate which is the reagent R2.
The preparation method of the emulsion-coated ferrocenium solution comprises the following steps:
s1, putting latex microspheres with the particle sizes of 65nm and 120nm into MES buffer solution, adding EDAC solution, mixing uniformly, incubating for 1.5h at 37 ℃, centrifuging to remove supernatant, adding MES buffer solution until the volume is unchanged, incubating for 1.5h at 37 ℃, centrifuging again, and removing supernatant to obtain latex solution;
s2, adding the mixture of the ferrous oxazine and the polyethylene p-chloromethyl styrene copolymer to react for 1-3h on the basis of the latex solution obtained in the step S1, centrifuging and precipitating, dispersing the precipitate in MES buffer solution, incubating for 2h at 37 ℃, centrifuging, dispersing and dissolving the precipitate in Tris buffer solution with the volume of one half of the total volume of the Tris buffer solution in a reagent R2, and sealing for 45h at 8 ℃ to obtain the latex coated ferrous oxazine solution.
Example 6 preparation of Total iron assay kit
In this example, the reagent R1 and the reagent R2 in example 3 are used as raw materials for preparing a total iron detection kit by the following preparation method:
step a), preparation of reagent R1:
adding purified water into a preparation tank A, sequentially adding a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum into the preparation tank, mixing and stirring, adjusting the pH value of the solution to 3 in the mixing process, stirring the mixture until the solution is clarified, and filtering by using a microporous filter membrane to obtain a filtrate as a reagent R1;
step B), preparation of reagent R2:
adding purified water into a preparation tank B, sequentially adding a Tris buffer solution, a latex coated ferrocenium solution and ascorbic acid into the preparation tank B, mixing and stirring until the solution is clear, and adjusting the pH value of the solution to 7.5;
and filtering the mixed solution by using a microporous filter membrane to obtain filtrate which is the reagent R2.
The preparation method of the emulsion-coated ferrocenium solution comprises the following steps:
s1, putting latex microspheres with the particle sizes of 65nm and 120nm into MES buffer solution, adding EDAC solution, mixing uniformly, incubating for 1.8h at 37 ℃, centrifuging to remove supernatant, adding MES buffer solution until the volume is unchanged, incubating for 1.8h at 37 ℃, centrifuging again to remove supernatant to obtain latex solution;
s2, adding the mixture of the ferrous oxazine and the polyethylene p-chloromethyl styrene copolymer (the final concentration of the polyethylene p-chloromethyl styrene copolymer in the reagent R2 is 10%, and the same is carried out in the following examples) to react for 1h on the basis of the latex solution obtained in the step S1, centrifuging and precipitating, dispersing the precipitate in MES buffer solution, incubating for 1.5h at 37 ℃, centrifuging, dispersing and dissolving the precipitate in Tris buffer solution which is one-half of the total volume of the Tris buffer solution in the reagent R2, and sealing for 36h at 6 ℃ to obtain the latex coated ferrous oxazine solution.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A total iron detection kit is characterized by comprising a reagent R1 and a reagent R2;
the components and concentrations in the reagent R1 were as follows:
the components and concentrations in the reagent R2 were as follows:
tris buffer solution 35-60mmol/L
2-4% of emulsion coating ferrioxamine solution
Ascorbic acid 15-30 mmol/L.
2. The total iron detection kit according to claim 1, wherein the components and concentrations in the reagent R1 are as follows:
3. the total iron detection kit according to claim 2, wherein the components and concentrations in the reagent R1 are as follows:
4. the total iron detection kit according to claim 1, wherein the components and concentrations in the reagent R2 are as follows:
tris buffer solution 48mmol/L
Latex-coated ferrioxamine solution 3%
Ascorbic acid 28 mmol/L.
5. The total iron detection kit according to claim 1, wherein the pH of the reagent R1 is between 2.0 and 3.5.
6. The total iron detection kit according to claim 1, wherein the pH of the reagent R2 is between 6.5 and 7.8.
7. A method for preparing a total iron detection kit according to any one of claims 1 to 6, comprising the steps of:
step a), preparation of reagent R1:
adding purified water into a preparation tank A, sequentially adding a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum into the preparation tank, mixing and stirring, adjusting the pH value of the solution to 3 in the mixing process, stirring the mixture until the solution is clarified, and filtering by using a microporous filter membrane to obtain a filtrate as a reagent R1;
step B), preparation of reagent R2:
adding purified water into a preparation tank B, sequentially adding a Tris buffer solution, a latex coated ferrocenium solution and ascorbic acid into the preparation tank B, mixing and stirring until the solution is clear, and adjusting the pH value of the solution to 7.5;
and filtering the mixed solution by using a microporous filter membrane to obtain filtrate which is the reagent R2.
8. The method for preparing a total iron detection kit according to claim 7, wherein the method for preparing the latex-coated ferrocenium solution is as follows:
s1, putting latex microspheres with the particle sizes of 65nm and 120nm into MES buffer solution, adding EDAC solution, mixing uniformly, incubating for 1.5-2h at 37 ℃, centrifuging to remove supernatant, supplementing MES buffer solution until the volume is unchanged, incubating for 1.5-2h at 37 ℃, centrifuging again, and removing supernatant to obtain latex solution;
s2, adding the mixture of the ferrous oxazine and the polyethylene p-chloromethyl styrene copolymer to react for 1-3h on the basis of the latex solution obtained in the step S1, carrying out centrifugal precipitation, dispersing the precipitate in MES buffer solution, incubating for 1.5-2h at 37 ℃, centrifuging, dispersing and dissolving the precipitate in a Tris buffer solution which is one half of the total volume of the Tris buffer solution in a reagent R2, and sealing for 36-45h at 2-8 ℃ to obtain the latex coated ferrous oxazine solution.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104483494A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Serum UIBC (unsaturated iron bonding capacity) detection kit |
| US20150192556A1 (en) * | 2012-07-27 | 2015-07-09 | Hach Company | Continuous turbidimetric total iron monitoring |
| CN107741494A (en) * | 2017-09-30 | 2018-02-27 | 安徽伊普诺康生物技术股份有限公司 | A kind of preparation method of CER detection kit |
| US20180099057A1 (en) * | 2015-03-19 | 2018-04-12 | Kansas State University Research Foundation | Nanoplatforms for arginase, indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase detection by posttranslational modification |
| CN110568206A (en) * | 2019-09-12 | 2019-12-13 | 苏州普瑞斯生物科技有限公司 | total iron binding force detection kit and preparation method thereof |
-
2020
- 2020-01-02 CN CN202010002556.XA patent/CN111122882A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150192556A1 (en) * | 2012-07-27 | 2015-07-09 | Hach Company | Continuous turbidimetric total iron monitoring |
| CN104483494A (en) * | 2014-12-22 | 2015-04-01 | 宁波美康生物科技股份有限公司 | Serum UIBC (unsaturated iron bonding capacity) detection kit |
| US20180099057A1 (en) * | 2015-03-19 | 2018-04-12 | Kansas State University Research Foundation | Nanoplatforms for arginase, indoleamine 2,3-dioxygenase and tryptophan 2,3-dioxygenase detection by posttranslational modification |
| CN107741494A (en) * | 2017-09-30 | 2018-02-27 | 安徽伊普诺康生物技术股份有限公司 | A kind of preparation method of CER detection kit |
| CN110568206A (en) * | 2019-09-12 | 2019-12-13 | 苏州普瑞斯生物科技有限公司 | total iron binding force detection kit and preparation method thereof |
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Application publication date: 20200508 |