CN111121622A - Biological reagent position identification method, device and system based on image acquisition - Google Patents
Biological reagent position identification method, device and system based on image acquisition Download PDFInfo
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- CN111121622A CN111121622A CN201911368688.8A CN201911368688A CN111121622A CN 111121622 A CN111121622 A CN 111121622A CN 201911368688 A CN201911368688 A CN 201911368688A CN 111121622 A CN111121622 A CN 111121622A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/002—Measuring arrangements characterised by the use of optical techniques for measuring two or more coordinates
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K7/00—Methods or arrangements for sensing record carriers, e.g. for reading patterns
- G06K7/10—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation
- G06K7/10544—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum
- G06K7/10821—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum further details of bar or optical code scanning devices
- G06K7/10861—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum further details of bar or optical code scanning devices sensing of data fields affixed to objects or articles, e.g. coded labels
Abstract
The embodiment of the invention discloses a biological reagent position identification method, a device and a system based on image acquisition, wherein the method comprises the following steps: the method comprises the steps of obtaining product information of a biological reagent to be stored, converting the product information into a two-dimensional code, and matching the two-dimensional code with test tubes of the biological reagent to be stored one by one; collecting image information of a hole plate in which a plurality of test tubes are placed; extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information; and matching and binding the hole site coordinates with the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate. The technical problem that in the prior art, the warehousing interval time of biological reagents is long due to manual input of position information is solved.
Description
Technical Field
The embodiment of the invention relates to the technical field of biological reagent storage, in particular to a method, a device and a system for identifying a biological reagent position based on image acquisition.
Background
With the development of the drug development industry, the use frequency of biological reagents in drug development is gradually increased, and the biological reagents need to be stored at low temperature, so that the operation time is short when the biological reagents are transferred or stored in a warehouse. In addition, since the biological reagents are generally stored in EP tubes or cryopreservation tubes, which have a small volume and cannot stand, they are generally stored in cryopreservation boxes or well plates with circular holes, such as 96-well plates and 384-well plates.
Because the pore plate is used for storing the biological reagents, the coordinates, the number of lines and the number of columns of the stored pores on the pore plate need to be detailed in the positioning of the biological reagents, then the pore plate is stored on the goods space of a warehouse shelf, and a claimant can quickly position and take the reagents according to the information when the reagent is claimed. In the prior art, the position information is usually entered by manual entry. However, since the biological reagents cannot be stored at normal temperature for a long time, the process of storing and positioning the test tubes in the storage is required to be relatively rapid, if the hole sites where the biological reagents are located in each tube are manually recorded, a lot of time is spent for inputting detailed position information, and the quality of the biological reagents is affected after the biological reagents are exposed to the normal temperature environment for a long time.
Disclosure of Invention
Therefore, the embodiment of the invention provides a method, a device and a system for identifying the position of a biological reagent based on image acquisition, which aim to solve the technical problem that the warehousing interval time of the biological reagent is long due to manual input of position information in the prior art.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:
a method of image acquisition-based biological agent location identification, the method comprising:
the method comprises the steps of obtaining product information of a biological reagent to be stored, converting the product information into a two-dimensional code, and matching the two-dimensional code with test tubes of the biological reagent to be stored one by one;
collecting image information of a hole plate in which a plurality of test tubes are placed;
extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information;
and matching and binding the hole site coordinates with the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate.
Further, acquire the product information of waiting to deposit biological reagent, convert the product information into the two-dimensional code to match the two-dimensional code with the test tube one-to-one of waiting to deposit this biological reagent, specifically include:
generating a code uniquely corresponding to each test tube according to the input product information of the biological reagent;
respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm;
and generating an image carrying two-dimensional code information according to the two-dimensional code.
Further, the extracting, according to the image information, the hole site coordinates and the two-dimensional code information where all the test tubes are located specifically includes:
extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm;
setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range;
correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format;
and extracting the text information contained in the two-dimensional code one by one, corresponding the text information to the corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information.
Further, the extracting, according to the image information, the hole site coordinates and the two-dimensional code information where all the test tubes are located specifically includes:
extracting shape features and specification features in the image information;
screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification;
and determining and marking the hole site coordinates.
Further, the matching and binding the hole site coordinates and the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate specifically includes:
and according to the coordinate range and the hole site coordinates of the two-dimensional code information, correlating the two-dimensional code information with the hole site information corresponding to the hole site coordinate closest to the center of the coordinate range where the two-dimensional code information is located, so as to obtain the position coordinates of each biological reagent on the hole plate.
The invention also provides an image acquisition-based biological reagent position identification device for implementing the method, which comprises the following steps:
the two-dimensional code generating unit is used for acquiring product information of the biological reagent to be stored, converting the product information into a two-dimensional code and matching the two-dimensional code with the test tubes of the biological reagent to be stored one by one;
the image acquisition unit is used for acquiring image information of the pore plate in which the test tubes are placed;
the coordinate positioning unit is used for extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information;
and the information matching unit is used for matching and binding the hole site coordinates and the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate.
Further, the two-dimensional code generating unit is specifically configured to:
generating a code uniquely corresponding to each test tube according to the input product information of the biological reagent;
respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm;
and generating an image carrying two-dimensional code information according to the two-dimensional code.
Further, the coordinate locating unit is specifically configured to:
extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm;
setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range;
correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format;
extracting text information contained in the two-dimensional codes one by one, enabling the text information to correspond to corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information;
extracting shape features and specification features in the image information;
screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification;
determining and marking the hole site coordinates;
the information matching unit is specifically configured to:
and according to the coordinate range and the hole site coordinates of the two-dimensional code information, correlating the two-dimensional code information with the hole site information corresponding to the hole site coordinate closest to the center of the coordinate range where the two-dimensional code information is located, so as to obtain the position coordinates of each biological reagent on the hole plate.
The invention also provides a biological reagent position identification system based on image acquisition, which comprises: a processor and a memory;
the memory is to store one or more program instructions;
the processor, configured to execute one or more program instructions to perform the method of any of claims 1-5.
The present invention also provides a computer storage medium containing one or more program instructions for performing a method as described above by a biological agent location identification system.
According to the image acquisition-based biological reagent position identification method, device and system, the product information of the biological reagent is stored in a two-dimensional code form, and the two-dimensional code with the product information is matched with the hole sites for storing the test tubes by means of image acquisition and feature extraction during storage, so that automatic matching and warehousing of the biological reagent and the storage hole sites are realized. Therefore, in the process of warehousing the biological agents, the time required by the mode of manually placing the biological agents, recording the position information of the biological agents one by one and warehousing and shelving the biological agents is shortened to be within half a minute or even ten seconds, the operation efficiency of warehousing and shelving the biological agents is obviously improved, the time is saved, and the time for warehousing and shelving the biological agents is shortened. Meanwhile, the time for warehousing and shelving the biological reagent is obviously shortened, and the exposure time of the biological reagent at normal temperature in the warehousing and shelving process is shortened, so that the deterioration risk of the biological reagent at normal temperature is reduced, and the quality of the reagent is guaranteed. In addition, the method can reduce the risk of biological reagents going bad in the warehousing racking or inventory transfer process, thereby ensuring the effect of subsequent experiments, and can quickly and accurately find the specific position of the biological reagents regardless of experimenters or inventory management personnel, thereby improving the use efficiency of the reagents, accelerating the scientific research process and reducing the scientific research cost. In general, the method solves the technical problem that the warehousing interval time of the biological reagent is long due to manual input of the position information in the prior art
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a flow chart of one embodiment of a method for identifying a biological reagent location based on image acquisition according to the present invention;
FIG. 2 is a block diagram of a biological reagent position recognition device based on image acquisition according to an embodiment of the present invention;
FIG. 3 is a block diagram of an embodiment of a biological reagent location identification system based on image acquisition according to the present invention.
Description of reference numerals:
100-two-dimensional code generation unit 200-image acquisition unit 300-coordinate positioning unit
400-information matching unit
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In a specific embodiment, the biological reagent position identification method based on image acquisition provided by the invention realizes timely warehousing of biological reagents by methods such as two-dimensional code generation, image acquisition and feature extraction, coordinate matching and the like, so as to solve the technical problem of long warehousing interval time of the biological reagents caused by manual inputting of position information in the prior art.
As shown in fig. 1, the method comprises the steps of:
s1: the method comprises the steps of obtaining product information of a biological reagent to be stored, converting the product information into a two-dimensional code, and matching the two-dimensional code with test tubes of the biological reagent to be stored one by one; it should be understood that a cuvette refers to a conventional container for holding biological reagents, such as an EP tube or a cryopreservation tube, and is collectively referred to herein as a cuvette.
Step S1 specifically includes generating a code uniquely corresponding to each test tube according to the product information of the entered biological reagent; respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm; and generating an image carrying two-dimensional code information according to the two-dimensional code.
That is, before the biological information needs to be acquired through the photo, the information of the biological reagent needs to be input into the system by means of importing or accessing other systems, which is called a pre-warehousing process. In the pre-warehousing process, after product information of the biological reagent is input into the system, the system respectively generates a unique corresponding code for each EP pipe or each cryopreservation pipe; and then generating two-dimensional code information from the corresponding codes through a two-dimensional code generation algorithm. The two-dimensional code information can contain thousands of character information, so that fields related to biological reagents, such as unique codes generated in the pre-warehousing process, and information such as names, specifications, batch numbers, manufacturers and prices can be put into the two-dimensional code information for subsequent systems. And generating a picture in the system by using the two-dimensional code, and printing the stickable label paper by using a bar code printer for sticking on an EP (EP) tube or a freezing tube of the biological reagent.
S2: collecting image information of a hole plate in which a plurality of test tubes are placed; it should be understood that the well plate herein refers to a container for holding test tubes, and may be embodied as a perforated plate or a freezing box. When image acquisition, because biological reagent puts on freezing box or orifice plate after, if the side is shot, the reagent pipe of back row can not shoot, need shoot from the top, just can shoot whole biological reagent pipe, consequently, the two-dimensional code label that the last step generated should paste on the lid of EP pipe or freezing pipe to guarantee that whole two-dimensional code picture is all complete on the lid, the condition of not being folded.
Specifically, the device for image acquisition can be a digital camera or a mobile device, and the like, and the image acquisition device takes a picture of the biological reagent EP pipe or the cryopreservation box or the pore plate of the cryopreservation pipe, and brings the whole cryopreservation box or the pore plate into the picture range, including all the two-dimensional codes, and the brightness requirement of the whole picture is higher, so that the brightness of each part is basically consistent, and the accuracy of the subsequent two-dimensional code identification is ensured. If the photo is taken by the mobile equipment, the photo taken by the equipment can be directly read in the photo album and read in the information identification module.
S3: extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information;
wherein, the extraction of two-dimensional code information specifically includes: extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm; setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range; correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format; and extracting the text information contained in the two-dimensional code one by one, corresponding the text information to the corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information.
Because the shot picture contains a plurality of two-dimensional code pictures and all the two-dimensional code information needs to be read out at one time, the common scanning function cannot be directly used (only one piece of two-dimensional code information can be obtained at one time). That is to say, after a shot picture is obtained, firstly, the two-dimension code positioning algorithm is used for extracting the position information accurate positioning information of all the two-dimension codes in the picture, then, the picture content in the two-dimension code coordinate range is intercepted one by one through the picture processing algorithm for correction and restoration, the picture content is processed into a standard two-dimension code picture format, then, the two-dimension code information identification algorithm is used for extracting the text information contained in the two-dimension codes one by one, and the text information and the corresponding coordinate information are in one-to-one correspondence and are recorded.
In step S3, extracting the hole site coordinates of all the test tubes according to the image information, specifically including: extracting shape features and specification features in the image information; screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification; and determining and marking the hole site coordinates. In the in-service use process, because the test tube is mostly circular structure, consequently, the hole site shape characteristic of discerning is mostly circular. Therefore, after the photographed picture is acquired, all circles in the picture are recognized by using a circle recognition algorithm in image processing, wherein the circles include a circular cover of a cryopreservation tube or an EP tube for storing a biological reagent, a round hole in a cryopreservation box or a well plate for storing the biological reagent, and the like. The circle recognition algorithm in the image processing technology can obtain the circle center coordinate and radius data of the recognized circle, and can remove some wrongly recognized circles according to the circle center coordinate position and radius size, so that only correct hole positions are recognized. After the hole sites are identified, the system records the coordinates and radius information of the circle centers of the hole sites, so that the number of the hole sites contained in the freezing storage box or the hole plate can be calculated and distributed into a plurality of rows and a plurality of columns, and the coordinates of each hole site are marked according to the serial numbers of the row and the column of each hole site.
S4: and matching and binding the hole site coordinates with the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate. Specifically, according to the coordinate range and the hole site coordinates of the two-dimensional code information, the two-dimensional code information is associated with the hole site information corresponding to the hole site coordinate closest to the center of the coordinate range where the two-dimensional code information is located, so as to obtain the position coordinates of each biological reagent on the hole plate.
In the actual use process, after coordinate positioning information of the biological reagent cryopreservation tube or the EP tube on the cryopreservation box or the pore plate is obtained by the method, the information can be returned to the inventory management system, the system records the information, and the warehousing and shelving process of the biological reagent is completed. After the process is finished, when a user inquires and receives the biological reagents, the user can immediately check the accurate position information of each cryopreservation tube or EP tube for storing the biological reagents on the shelf and in the cryopreservation box or the pore plate, so that the biological information can be quickly acquired to carry out the subsequent working process. The method comprises the steps of taking a picture of a cryopreservation box or a pore plate in which a cryopreservation tube or an EP tube is placed once, and then finishing accurate positioning of the cryopreservation tube or the EP tube on the cryopreservation box or the pore plate through an image processing technology (a two-dimensional code recognition technology and a circular recognition technology). The whole positioning process is automatically completed by the system background without manual intervention, and the whole process can be completed in 1 to 2 seconds and the final information is transmitted to the inventory management system, so that the time for storing the biological reagent stored in the way into a warehouse and putting the biological reagent on a shelf is greatly shortened.
In the above embodiment, the image-acquisition-based biological reagent position identification method, device and system provided by the invention have the advantages that the product information of the biological reagent is stored in the form of the two-dimensional code, and the two-dimensional code with the product information is matched with the hole sites for storing the test tubes by using the image acquisition and feature extraction means during storage, so that the biological reagent and the storage hole sites are automatically matched and stored in a warehouse. Therefore, in the process of warehousing the biological agents, the time required by the mode of manually placing the biological agents, recording the position information of the biological agents one by one and warehousing and shelving the biological agents is shortened to be within half a minute or even ten seconds, the operation efficiency of warehousing and shelving the biological agents is obviously improved, the time is saved, and the time for warehousing and shelving the biological agents is shortened. Meanwhile, the time for warehousing and shelving the biological reagent is obviously shortened, and the exposure time of the biological reagent at normal temperature in the warehousing and shelving process is shortened, so that the deterioration risk of the biological reagent at normal temperature is reduced, and the quality of the reagent is guaranteed. In addition, the method can reduce the risk of biological reagents going bad in the warehousing racking or inventory transfer process, thereby ensuring the effect of subsequent experiments, and can quickly and accurately find the specific position of the biological reagents regardless of experimenters or inventory management personnel, thereby improving the use efficiency of the reagents, accelerating the scientific research process and reducing the scientific research cost.
In addition to the above method, the present invention also provides an image-capturing-based biological reagent location identification apparatus for implementing the method as described above, as shown in fig. 2, the apparatus comprising:
the two-dimensional code generating unit 100 is configured to obtain product information of a biological reagent to be stored, convert the product information into a two-dimensional code, and match the two-dimensional code with test tubes in which the biological reagent is to be stored one by one; it should be understood that a cuvette refers to a conventional container for holding biological reagents, such as an EP tube or a cryopreservation tube, and is collectively referred to herein as a cuvette.
The two-dimensional code generating unit converts the codes into two-dimensional codes respectively through a two-dimensional code generating algorithm; and generating an image carrying two-dimensional code information according to the two-dimensional code. That is, before the biological information needs to be acquired through the photo, the information of the biological reagent needs to be input into the system by means of importing or accessing other systems, which is called a pre-warehousing process. In the pre-warehousing process, after product information of the biological reagent is input into the system, the system respectively generates a unique corresponding code for each EP pipe or each cryopreservation pipe; and then generating two-dimensional code information from the corresponding codes through a two-dimensional code generation algorithm. The two-dimensional code information can contain thousands of character information, so that fields related to biological reagents, such as unique codes generated in the pre-warehousing process, and information such as names, specifications, batch numbers, manufacturers and prices can be put into the two-dimensional code information for subsequent systems. And generating a picture in the system by using the two-dimensional code, and printing the stickable label paper by using a bar code printer for sticking on an EP (EP) tube or a freezing tube of the biological reagent.
An image collecting unit 200 for collecting image information of the well plate in which the plurality of test tubes are placed; it should be understood that the well plate herein refers to a container for holding test tubes, and may be embodied as a perforated plate or a freezing box. When image acquisition, because biological reagent puts on freezing box or orifice plate after, if the side is shot, the reagent pipe of back row can not shoot, need shoot from the top, just can shoot whole biological reagent pipe, consequently, the two-dimensional code label that the last step generated should paste on the lid of EP pipe or freezing pipe to guarantee that whole two-dimensional code picture is all complete on the lid, the condition of not being folded.
Specifically, the device for image acquisition can be a digital camera or a mobile device, and the like, and the image acquisition device takes a picture of the biological reagent EP pipe or the cryopreservation box or the pore plate of the cryopreservation pipe, and brings the whole cryopreservation box or the pore plate into the picture range, including all the two-dimensional codes, and the brightness requirement of the whole picture is higher, so that the brightness of each part is basically consistent, and the accuracy of the subsequent two-dimensional code identification is ensured. If the photo is taken by the mobile equipment, the photo taken by the equipment can be directly read in the photo album and read in the information identification module.
The coordinate positioning unit 300 is used for extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information; wherein, the extraction of two-dimensional code information specifically includes: extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm; setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range; correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format; and extracting the text information contained in the two-dimensional code one by one, corresponding the text information to the corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information.
Because the shot picture contains a plurality of two-dimensional code pictures and all the two-dimensional code information needs to be read out at one time, the common scanning function cannot be directly used (only one piece of two-dimensional code information can be obtained at one time). That is to say, after a shot picture is obtained, firstly, the two-dimension code positioning algorithm is used for extracting the position information accurate positioning information of all the two-dimension codes in the picture, then, the picture content in the two-dimension code coordinate range is intercepted one by one through the picture processing algorithm for correction and restoration, the picture content is processed into a standard two-dimension code picture format, then, the two-dimension code information identification algorithm is used for extracting the text information contained in the two-dimension codes one by one, and the text information and the corresponding coordinate information are in one-to-one correspondence and are recorded.
The coordinate locating unit 300 specifically includes, when extracting the hole site coordinate: extracting shape features and specification features in the image information; screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification; and determining and marking the hole site coordinates. In the in-service use process, because the test tube is mostly circular structure, consequently, the hole site shape characteristic of discerning is mostly circular. Therefore, after the photographed picture is acquired, all circles in the picture are recognized by using a circle recognition algorithm in image processing, wherein the circles include a circular cover of a cryopreservation tube or an EP tube for storing a biological reagent, a round hole in a cryopreservation box or a well plate for storing the biological reagent, and the like. The circle recognition algorithm in the image processing technology can obtain the circle center coordinate and radius data of the recognized circle, and can remove some wrongly recognized circles according to the circle center coordinate position and radius size, so that only correct hole positions are recognized. After the hole sites are identified, the system records the coordinates and radius information of the circle centers of the hole sites, so that the number of the hole sites contained in the freezing storage box or the hole plate can be calculated and distributed into a plurality of rows and a plurality of columns, and the coordinates of each hole site are marked according to the serial numbers of the row and the column of each hole site.
And the information matching unit 400 is used for matching and binding the hole site coordinates and the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate. Specifically, the information matching unit is used for generating codes uniquely corresponding to the test tubes according to the input product information of the biological reagent; respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm; and generating an image carrying two-dimensional code information according to the two-dimensional code.
In the above embodiment, the image-acquisition-based biological reagent position recognition device provided by the invention is used for matching the two-dimensional code with the product information with the hole site for storing the test tube by transferring the product information of the biological reagent into the form of the two-dimensional code and by using image acquisition and feature extraction means during storage, thereby realizing automatic matching and warehousing of the biological agent and the storage hole site. Therefore, in the process of warehousing the biological agents, the time required by the mode of manually placing the biological agents, recording the position information of the biological agents one by one and warehousing and shelving the biological agents is shortened to be within half a minute or even ten seconds, the operation efficiency of warehousing and shelving the biological agents is obviously improved, the time is saved, and the time for warehousing and shelving the biological agents is shortened. Meanwhile, the time for warehousing and shelving the biological reagent is obviously shortened, and the exposure time of the biological reagent at normal temperature in the warehousing and shelving process is shortened, so that the deterioration risk of the biological reagent at normal temperature is reduced, and the quality of the reagent is guaranteed. In addition, the method can reduce the risk of biological reagents going bad in the warehousing racking or inventory transfer process, thereby ensuring the effect of subsequent experiments, and can quickly and accurately find the specific position of the biological reagents regardless of experimenters or inventory management personnel, thereby improving the use efficiency of the reagents, accelerating the scientific research process and reducing the scientific research cost.
According to a third aspect of the embodiments of the present invention, the present invention further provides an image-capturing-based biological agent location identification system, as shown in fig. 3, the system comprising: a processor 201 and a memory 202;
the memory is to store one or more program instructions;
the processor is configured to execute one or more program instructions to perform the method as described above.
In correspondence with the above embodiments, embodiments of the present invention also provide a computer storage medium containing one or more program instructions therein. Wherein the one or more program instructions are for execution by a biological agent location identification system to perform the method as described above.
In an embodiment of the invention, the processor may be an integrated circuit chip having signal processing capability. The Processor may be a general purpose Processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA) or other programmable logic device, discrete Gate or transistor logic device, discrete hardware component.
The various methods, steps and logic blocks disclosed in the embodiments of the present invention may be implemented or performed. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. The steps of the method disclosed in connection with the embodiments of the present invention may be directly implemented by a hardware decoding processor, or implemented by a combination of hardware and software modules in the decoding processor. The software module may be located in ram, flash memory, rom, prom, or eprom, registers, etc. storage media as is well known in the art. The processor reads the information in the storage medium and completes the steps of the method in combination with the hardware.
The storage medium may be a memory, for example, which may be volatile memory or nonvolatile memory, or which may include both volatile and nonvolatile memory.
The nonvolatile Memory may be a Read-Only Memory (ROM), a Programmable ROM (PROM), an Erasable PROM (EPROM), an Electrically Erasable PROM (EEPROM), or a flash Memory.
The volatile Memory may be a Random Access Memory (RAM) which serves as an external cache. By way of example, and not limitation, many forms of RAM are available, such as Static Random Access Memory (SRAM), Dynamic RAM (DRAM), Synchronous DRAM (SDRAM), Double Data Rate SDRAM (DDRSDRAM), Enhanced SDRAM (ESDRAM), SLDRAM (SLDRAM), and Direct Rambus RAM (DRRAM).
The storage media described in connection with the embodiments of the invention are intended to comprise, without being limited to, these and any other suitable types of memory.
Those skilled in the art will appreciate that the functionality described in the present invention may be implemented in a combination of hardware and software in one or more of the examples described above. When software is applied, the corresponding functionality may be stored on or transmitted over as one or more instructions or code on a computer-readable medium. Computer-readable media includes both computer storage media and communication media including any medium that facilitates transfer of a computer program from one place to another. A storage media may be any available media that can be accessed by a general purpose or special purpose computer.
The above embodiments are only for illustrating the embodiments of the present invention and are not to be construed as limiting the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made on the basis of the embodiments of the present invention shall be included in the scope of the present invention.
Claims (10)
1. A method for identifying a biological reagent position based on image acquisition, the method comprising:
the method comprises the steps of obtaining product information of a biological reagent to be stored, converting the product information into a two-dimensional code, and matching the two-dimensional code with test tubes of the biological reagent to be stored one by one;
collecting image information of a hole plate in which a plurality of test tubes are placed;
extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information;
and matching and binding the hole site coordinates with the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate.
2. The method for identifying the position of the biological reagent according to claim 1, wherein the step of obtaining the product information of the biological reagent to be stored, converting the product information into the two-dimensional code, and matching the two-dimensional code with the test tubes of the biological reagent to be stored one by one specifically comprises the steps of:
generating a code uniquely corresponding to each test tube according to the input product information of the biological reagent;
respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm;
and generating an image carrying two-dimensional code information according to the two-dimensional code.
3. The method for identifying the position of a biological reagent according to claim 1, wherein the extracting the hole site coordinates and the two-dimensional code information of all the test tubes according to the image information specifically comprises:
extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm;
setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range;
correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format;
and extracting the text information contained in the two-dimensional code one by one, corresponding the text information to the corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information.
4. The method for identifying the position of a biological reagent according to claim 3, wherein the extracting the hole site coordinates and the two-dimensional code information of all the test tubes according to the image information specifically comprises:
extracting shape features and specification features in the image information;
screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification;
and determining and marking the hole site coordinates.
5. The method for identifying the positions of biological reagents according to claim 4, wherein the step of matching and binding the hole position coordinates with the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate specifically comprises the steps of:
and according to the coordinate range and the hole site coordinates of the two-dimensional code information, correlating the two-dimensional code information with the hole site information corresponding to the hole site coordinate closest to the center of the coordinate range where the two-dimensional code information is located, and obtaining the position coordinates of each biological reagent on the hole plate.
6. An image acquisition-based biological agent location identification device for implementing the method according to any one of claims 1 to 5, wherein the device comprises:
the two-dimensional code generating unit is used for acquiring product information of the biological reagent to be stored, converting the product information into a two-dimensional code and matching the two-dimensional code with the test tubes of the biological reagent to be stored one by one;
the image acquisition unit is used for acquiring image information of the pore plate in which the test tubes are placed;
the coordinate positioning unit is used for extracting hole site coordinates and two-dimensional code information of all the test tubes according to the image information;
and the information matching unit is used for matching and binding the hole site coordinates and the two-dimensional code information one by one to obtain the position coordinates of each biological reagent on the pore plate.
7. The biological reagent site recognition device of claim 6, wherein the two-dimensional code generation unit is specifically configured to:
generating a code uniquely corresponding to each test tube according to the input product information of the biological reagent;
respectively converting each code into a two-dimensional code through a two-dimensional code generation algorithm;
and generating an image carrying two-dimensional code information according to the two-dimensional code.
8. The bioreagent location identification device of claim 6, wherein the coordinate positioning unit is specifically configured to:
extracting the position information of all the two-dimension codes in the image information through a two-dimension code positioning algorithm;
setting a two-dimensional code coordinate, and intercepting picture content in the two-dimensional code coordinate range;
correcting and restoring the intercepted picture content to obtain a standard two-dimensional code picture format;
extracting text information contained in the two-dimensional codes one by one, enabling the text information to correspond to corresponding coordinate information one by one, and determining the coordinate range of the two-dimensional code information;
extracting shape features and specification features in the image information;
screening to obtain hole site coordinates corresponding to the test tube hole sites through comparison of the shape characteristics and the preset shape and comparison of the specification characteristics and the preset specification;
determining and marking the hole site coordinates;
the information matching unit is specifically configured to:
and according to the coordinate range and the hole site coordinates of the two-dimensional code information, correlating the two-dimensional code information with the hole site information corresponding to the hole site coordinate closest to the center of the coordinate range where the two-dimensional code information is located, so as to obtain the position coordinates of each biological reagent on the hole plate.
9. An image acquisition-based biological agent location identification system, the system comprising: a processor and a memory;
the memory is to store one or more program instructions;
the processor, configured to execute one or more program instructions to perform the method of any of claims 1-5.
10. A computer storage medium comprising one or more program instructions for use by a biological agent location identification system in performing the method of any of claims 1-5.
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