CN111117913A - High-salt-resistance high-COD wastewater degrading bacterial strain, culture method, bacterial liquid and application - Google Patents
High-salt-resistance high-COD wastewater degrading bacterial strain, culture method, bacterial liquid and application Download PDFInfo
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Abstract
The invention discloses a high-salt-resistance high-COD wastewater degrading bacterial strain, a culture method, a bacterial liquid and application. The strain is obtained by separating sludge near a sewage discharge outlet of the wastewater and performing circular domestication. The strain is identified as Rhodococcus erythropolis (R) ((R))Rhodococcus erythropolis). The strain can effectively degrade COD (the highest load of influent COD can reach 12000mg/L) in wastewater under the environment of higher salinity (the highest load of wastewater salt concentration can reach 80 g/L), the removal rate of COD can reach 62%, and effluent reaches the national comprehensive sewage discharge standard and is discharged into town for sewage treatmentAnd (5) the requirement of a factory for collecting the sewage of a pipe network. The treatment process is simple to operate, the effluent quality is good, the treatment cost is low, and engineering application can be carried out on the basis.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a high-salt-resistance high-COD wastewater degrading bacterial strain, a culture method, a bacterial liquid and application.
Background
COD (chemical Oxygen demand), which is the Chemical Oxygen Demand (COD), is also called chemical Oxygen Consumption (COD), and is used for reflecting the amount of reducing substances to be oxidized in the sewage, is an important organic pollution parameter which can be measured quickly in the research of river pollution and industrial sewage properties and the operation management of sewage treatment plants. With the remarkable increase of the modernization process in China, large-scale wastewater with high salinity, such as industrial wastewater, agricultural water, urban sewage and the like, is directly or indirectly discharged into the nature, and causes pollution to the environment such as the atmosphere, soil, water body and the like to different degrees. The COD in these wastewaters is complex and typically includes salts, hydrocarbons, organic heavy metals, and the like. The organic wastewater containing salt is classified into chloride, sulfate, calcium salt, sodium salt, etc. according to the type of salt.
At present, the organic wastewater treatment technology comprises a physical and chemical method, a biological method and the like. The physical and chemical methods mainly include adsorption, membrane separation, thermal separation, coagulation, ion exchange, oxidation, and the like. The biological method is mainly characterized in that under the aerobic or anaerobic condition, the growth and metabolism of microorganisms are utilized to open and decompose organic matters in the wastewater, so that the wastewater is purified.
When the salinity in the environment exceeds a certain critical range, the growth of microorganisms is inhibited and poisoned, the extracellular osmotic pressure can be increased along with the increase of the salt concentration, the intracellular pressure is lower, the water in the cells can flow out, and the plasmolysis is caused to cause the death of the microorganisms; in addition, salting-out occurs in the strains with overhigh salinity, and dehydrogenase in vivo is inhibited; when high salinity wastewater is treated, a plurality of microorganisms cannot survive in the severe environment, so that activated sludge floats upwards, and the purification effect is influenced; if a large amount of fresh water is used for dilution, not only the treatment cost is increased, but also the problem of shortage of fresh water is further aggravated. Therefore, it is very important and urgent to research the microbial strains and COD degradation process capable of treating industrial wastewater under higher salt.
Disclosure of Invention
In view of the disadvantages of the prior art, the first object of the present invention is to provide a wastewater-degrading bacterial strain which has the advantage of being able to tolerate high salinity and high COD concentration.
The second purpose of the invention is to provide a culture method of the high-salt-resistance high-COD wastewater degrading strain, which has the advantages of efficiently and quickly screening the high-salt-resistance high-COD wastewater degrading strain and ensuring the stability of the high-salt-resistance high-COD wastewater degrading strain.
The third purpose of the invention is to provide a bacterial liquid prepared by the high-salt-resistant high-COD wastewater degrading bacterial strain, and the bacterial liquid is applied to degrading COD in high-salt high-COD wastewater.
The fourth purpose of the invention is to provide the application of the high-salt-resistance high-COD wastewater degrading bacterium, the bacterium has simple operation on the treatment process of the high-salt high-COD wastewater, the effluent quality is good, the treatment cost is low, and the effluent can meet the requirements of the national sewage comprehensive discharge standard on sewage discharged into a collecting pipe network of a town sewage treatment plant.
In order to achieve the first object, the invention provides the following technical scheme: a degrading bacterial strain for high-salt-resistance high-COD wastewater is Rhodococcus erythropolis (Rhodococcus erythropolis)Rhodococcus erythropolis) Is named asRhodococcus erythropolisHQC-1, deposited at the China general microbiological culture Collection center (CGMCC), address: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19104 and preserving for the following time: 12 and 6 in 2019.
Furthermore, the bacterial strain shows positive through gram staining, bacterial colonies are round when the bacterial strain is cultured on a flat plate, the surface is smooth and slightly convex, the edge is regular, the color is red, and the bacterial strain is spherical through microscopic examination.
Further, the strain is selected from sludge near a sewage drain outlet.
Further, the screening and domestication of the strain comprises the following steps:
(1) collecting a sludge sample near a wastewater discharge outlet, inoculating the sludge sample on a beef extract liquid culture medium with the sodium sulfate concentration of 20g/L according to the inoculation amount of 2-10%, and carrying out enrichment culture at the temperature of 30 ℃ to obtain an acclimation culture solution;
(2) circularly inoculating the domestication culture solution obtained in the step (1) on a beef extract liquid culture medium with the sodium sulfate concentration increased by 10g/L each time, and circularly domesticating until the salt concentration in the beef extract liquid culture medium reaches 80 g/L;
(3) and separating out the strains in the finally obtained domestication culture solution to obtain the strains of the sulfate-resistant strains.
The high-salt-resistant high-COD strain is screened from sludge near a wastewater discharge outlet, the sludge contains various microorganisms, generally, the wastewater contains high salt and high COD, when the wastewater is discharged from the wastewater discharge outlet for a long time, the microorganisms in the sludge near the wastewater discharge outlet are contacted with the high-salt high-COD wastewater for a long time, part of the microorganisms cannot survive due to the severe environment of high salt and high COD, and the rest of the microorganisms adapt to the environment of high salt and high COD gradually, so that the microorganisms can normally survive in the severe environment. The bacterial strains are screened and domesticated on the basis of sulfate, on one hand, the sulfate is common salt which is difficult to treat in the wastewater, so that the survived microorganisms in the sludge near the sewage discharge outlet of the sampled wastewater have better tolerance to the sulfate to a great extent, the screening efficiency of the microorganisms can be improved, and on the other hand, the screened strains under the condition have better effect on the corresponding treatment in the subsequent sulfate wastewater.
Further, the strain can tolerate high salt in the range of 40-80 g/L, and the strain can tolerate high COD in the range of 4000-12000 mg/L.
After the microorganisms with high salt and high COD are screened, the tolerance range of the microorganisms with high salt and high COD is researched, and the research result shows that the bacteria can tolerate the high salt up to 80 g/L and the tolerant high COD up to 12000 mg/L.
In order to achieve the second object, the invention provides the following technical scheme: a method for culturing a high-salt-resistance high-COD wastewater degrading bacterial strain comprises the following steps of inoculating the bacterial strain into a beef extract liquid culture medium according to the inoculation amount of 2-10%, wherein the components of the culture medium are as follows: 0.1-1.0% of beef extract; peptone 01-3.0%; 0.1 to 1.0 percent of sodium chloride; potassium dihydrogen phosphate 0.01-1.0%; magnesium sulfate 0.01-1.0%, adjusting pH to 6.0-8.0, culturing at 20-45 deg.C for 16-32 hr in constant temperature shaking incubator at 20-45 deg.C, and selecting OD600The bacterial liquid of 0.4-1.8 is bacterial liquid of bacterial strain.
The research is carried out on the concentration ratio of each component of a beef extract liquid culture medium for culturing the screened strain, the culture temperature of the strain and the culture pH, so that the strain with high salt resistance and high COD (chemical oxygen demand) resistance can be normally cultured under the culture medium components and the culture conditions, and the content of available viable bacteria obtained after the culture under normal conditions is not less than 2 x 109one/mL. For the subsequent further pairsRhodococcus erythropolis.In the process of degrading COD in wastewater by HQC-1, the effective viable bacteria content of the inoculated strain unit also has great influence on the degradation of COD in the wastewater. Therefore, when the strain is inoculated to high-salt high-COD wastewater for COD degradation, the effective viable cell content per unit of the inoculated strain is larger than or equal to 2X 109one/mL is used as a control index.
In order to achieve the third purpose, the invention provides a bacterial liquid prepared by adopting the high-salt-resistant high-COD wastewater degrading bacterial strain.
Further, the process of preparing the bacterial liquid comprises the following steps of inoculating the bacterial strain into a beef extract liquid culture medium according to the inoculation amount of 2-10%, culturing for 16-32 hours in a constant-temperature shaking incubator at the temperature of 20-45 ℃, and selecting the bacterial liquid with the OD600 of 0.4-1.8 as the bacterial liquid of the bacterial strain.
By adopting the technical scheme, the bacterial liquid of the screened bacterial strains is prepared, and the bacterial liquid can be used for effectively degrading COD in high-salt and high-COD wastewater.
In order to achieve the fourth object, the invention provides the following technical solutions: an application of a high-salt-resistance high-COD wastewater degrading bacterial strain is applied to removal of COD in high-salt high-COD wastewater.
Further, the strain is added into the high-salt and high-COD wastewater according to the inoculation amount of 2-10% to remove COD, the temperature of the strain for removing the COD of the wastewater is 30-40 ℃, and the pH value is 6.0-8.0.
The screened strain is applied to the treatment of high-salt high-COD wastewater, the treatment process is optimized, the strain is added into the high-salt (sodium sulfate 60 g/L) high-COD (COD 7000-12000 mg/L) wastewater according to the inoculation amount of 2-10%, and the inoculated strainRhodococcus erythropolis.The effective viable cell content of HQC-1 is ≧ 2 × 109The removal rate of COD is more than 60 percent per ml.
In conclusion, the invention provides a high-salt-resistance high-COD wastewater degrading bacterial strain, and provides a culture method of the bacterial strain and an optimal treatment process for degrading COD in high-salt high-COD wastewater. The strain is identified as Rhodococcus erythropolis (R) ((R))Rhodococcus erythropolis) HQC-1, the strain can effectively degrade COD in wastewater in a high salinity environment, the removal rate of the COD can reach 62%, and the effluent reaches the requirements of national sewage comprehensive discharge standard on sewage discharged into a town sewage treatment plant collection pipe network. The treatment process is simple to operate, the effluent quality is good, the treatment cost is low, and engineering application can be carried out on the basis.
Drawings
FIG. 1 shows a schematic view of the present inventionRhodococcus erythropolis.Growth curve of HQC-1.
FIG. 2 shows a view of the present inventionRhodococcus erythropolis.The change curve of the COD degradation rate of the HQC-1 added into the wastewater along with the change of the inoculum size.
FIG. 3 shows a view of the present inventionRhodococcus erythropolis.The change curve of the COD degradation rate of the HQC-1 added with the wastewater along with the change of the original salt concentration of the wastewater.
FIG. 4 shows a view of the present inventionRhodococcus erythropolis.The change curve of the COD degradation rate of the HQC-1 added with the wastewater along with the change of the pH value of the wastewater.
FIG. 5 shows a view of the present inventionRhodococcus erythropolis.Change curve of COD degradation rate after HQC-1 is added into the wastewater along with the change of temperature.
FIG. 6 shows a view of the present inventionRhodococcus erythropolis.The change curve of the COD degradation rate of the HQC-1 added with the wastewater along with the change of the original COD concentration.
Detailed Description
The present invention providesA high-salt-resistance high-COD wastewater degrading bacterial strain is provided, and a culture method of the bacterial strain and an optimal treatment process for removing COD in high-salt high-COD wastewater are provided. The strain is obtained by separating sludge near a wastewater discharge outlet of a coal chemical industry group Limited company in the Luqi cities of Shanxi province and performing circular domestication. The strain is identified as Rhodococcus erythropolis by 16S r RNA (R & lt & gt)Rhodococcus erythropolis) Is named asRhodococcus erythropolis.HQC-1, preserved in China general microbiological culture Collection center (CGMCC), with the address: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19104 and preserving for the following time: 12 and 6 in 2019.
Wherein, in a beef extract liquid culture medium (which can be obtained commercially) used for domesticating the strains, the rest are conventional components and conventional proportions except the sulfate concentration.
The present invention will be described in further detail with reference to the following drawings and examples.
Example one
The domestication and screening steps of the high-salt and high-COD resistant wastewater degrading bacterial strain are as follows:
(1) collecting sludge from the vicinity of a wastewater discharge outlet of Tianqian coal chemical group Limited in Luqi cities of Shanxi province, inoculating a sludge sample on a beef extract liquid culture medium with the sulfate concentration of 10g/L in an inoculation amount of 5%, and carrying out enrichment culture on the mixture for 4 days at 30 ℃ on a shaking table to obtain a primary acclimation culture solution;
(2) inoculating the primary acclimation culture solution to a beef extract liquid culture medium with the sodium sulfate concentration of 20g/L according to the inoculation amount of the step (1), and carrying out enrichment culture on the beef extract liquid culture medium for 4 days at the temperature of 30 ℃ on a shaking table to obtain a secondary acclimation culture solution;
(3) inoculating the previous domestication culture solution to a new beef extract liquid culture medium containing sodium sulfate according to the inoculation amount in the step (1), increasing the concentration of sodium sulfate in the new culture medium by 10g/L each time, and carrying out enrichment culture on the new culture medium for 4 days at 30 ℃ on a shaking table to obtain a new first domestication culture solution;
(4) repeating the step (3), and performing circular domestication until the concentration of sodium sulfate in the liquid culture medium of the beef extract reaches 80 g/L;
(5) and separating out the strain in the finally obtained domestication culture solution, inoculating the strain into a beef extract liquid culture medium for culturing the strain, and standing at 30 ℃ for constant-temperature culture for 60 hours to obtain the sodium sulfate-resistant strain.
Example two
Molecular biological characterization of strains
The bacterial strain with sodium sulfate resistance is obtained through screening and domestication, gram staining shows positive, bacterial colony is round when plate culture is carried out, the surface is smooth and has slight bulge, the edge is regular, the color is red, and the bacterial body is spherical through microscopic examination. Identification of Rhodococcus erythropolis by 16S r RNA: (Rhodococcus erythropolis) Is named asRhodococcus erythropolis.HQC-1, the conditions of the survival, growth and reproduction of the strain are 20-55 ℃ and pH 6.0-8.0. The sequence listing in the identification of 16rRNA of the strain of the invention is shown in the appendix.
EXAMPLE III
Rhodococcus erythropolis.HQC-1 culture method
The separated bacterial strain is cultured by adopting a beef extract liquid culture medium, and the composition of the bacterial strain is as follows: 0.3% of beef extract; peptone 1.0%; 0.5 percent of sodium chloride; potassium dihydrogen phosphate 0.1%; 0.05 percent of magnesium sulfate; the culture temperature is 30 ℃; pH 7.2, OD of the Strain600The trend of the change with time (i.e. the growth curve of the strain) is shown in FIG. 1, and during the cultivation for 30h, the growth situation of the strain is found to be better within the period of 6-18h, therefore, the corresponding OD is selected600The bacterial liquid of 0.4-1.8 is bacterial liquid of strain, and has effective viable bacteria content ≧ 2 × 109one/mL.
Example four
Through the screening and domestication of the strains in the sludge near the sewage discharge port of the wastewater, the high-salt-resistant high-COD wastewater degrading bacterium is obtainedRhodococcus erythropolis.HQC-1 and is namedRhodococcus erythropolis.The culture medium concentration and the culture conditions of HQC-1 are further researched to obtain the optimized culture method of the strain. The strain is used for treating high-salt high-COD wastewater, and the strain is found to degrade COD in the high-salt high-COD wastewaterHas obvious effect, so the condition of the strain for degrading COD in the wastewater is further optimized, and the optimization process is further detailed below.
(1) Optimization of strain inoculation amount
Based on the organic wastewater with the original salt concentration of 40 g/L, COD and the concentration of 4000mg/L, the fermentation liquor of the salt-tolerant strain (the effective viable bacteria content is not less than 2 multiplied by 10)9piece/mL) are respectively inoculated into a 500mL conical flask containing 200mL of wastewater according to the inoculation amounts of 0mL (0%), 2mL (1%), 4mL (2%), 6mL (3%), 8mL (4%), 10mL (5%), 15mL (7.5%) and 20mL (10%), the sample inoculated with the strain is placed in a constant temperature shaking box at 30 ℃ and 200 r/min for culture, the degradation condition of COD in the wastewater under each inoculation amount is detected after 24 hours, and a change curve of COD degradation rate along with the change of the inoculation amount is drawn, as shown in FIG. 2. As can be seen from FIG. 2, the COD removal rate increased with the increase of the inoculum size; when the inoculation amount is 6mL (3%), the COD removal rate reaches 45%; as the amount of inoculum was increased, the COD removal rate decreased with increasing amount of inoculum.
(2) Optimization of raw salt concentration of wastewater
Based on organic wastewater with COD concentration of 4000mg/L, fermentation liquor of salt-tolerant strain (effective viable bacteria content ≧ 2 × 10)9And (2) inoculating the strain to 500mL conical flasks which respectively have original salt concentrations of 0g/L (0%), 20g/L (2%), 40 g/L (4%), 60g/L (6%), 80 g/L (8%) and 100 g/L (10%) and contain 200mL of organic wastewater according to the optimal inoculation amount obtained in the step (1), culturing in a constant-temperature shaking box at 30 ℃ and 200 r/min, detecting the degradation condition of COD of the wastewater under various salinity after 24 h, and drawing a change curve of COD degradation rate along with the change of the original salt concentration of the wastewater, wherein the change curve is shown in figure 3. As can be seen from FIG. 3, the COD removal rate increased with the increase of the salt concentration; when the salt concentration is 40 g/L (4%), the COD removal rate reaches 63%; as the salt concentration continues to increase, the COD removal rate decreases with increasing salt concentration.
(3) Optimization of wastewater pH
The organic wastewater with the optimal wastewater salt concentration and COD concentration of 4000mg/L obtained in the step (2) is used asBased on that, the fermentation liquor of the salt-tolerant strain (the effective viable bacteria content is not less than 2 multiplied by 10)9And (2) inoculating the strain to a 500mL conical flask containing 200mL of organic wastewater according to the optimal inoculation amount obtained in the step (1), wherein the pH of the organic wastewater is 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 respectively, culturing at the constant temperature of 30 ℃ in a shaking box at 200 r/min, detecting the degradation condition of COD (chemical oxygen demand) in the wastewater under each pH condition after 24 h, and drawing a change curve of the COD degradation rate along with the change of the pH of the wastewater, as shown in FIG. 4. As can be seen from FIG. 4, the COD removal rate increased with the increase in pH; when the pH value is 7.0, the COD removal rate reaches 69 percent; as pH continues to increase, COD removal rate decreases with increasing pH.
(4) Optimization of process temperature
Based on the organic wastewater with the optimal wastewater salt concentration and COD concentration of 4000mg/L obtained in the step (2), the fermentation liquor of the salt-tolerant strain (the effective viable bacteria content is not less than 2 multiplied by 10)9And (2) inoculating the strain to a 500mL conical flask containing 200mL of organic wastewater according to the optimal inoculation amount obtained in the step (1), adjusting the pH of the organic wastewater to the optimal pH obtained in the step (3), culturing in a constant-temperature shaking box at 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃ and 45 ℃ respectively, wherein the shaking rate of the constant-temperature shaking box is 200 r/min, detecting the degradation condition of COD (chemical oxygen demand) in the wastewater under various temperature conditions after 24 h, and drawing a change curve of the COD degradation rate along with the change of the temperature, as shown in FIG. 5. As can be seen from FIG. 5, the COD removal rate increased with increasing temperature; when the temperature is 35 ℃, the COD removal rate reaches 69.5 percent; as the temperature continues to increase, the COD removal rate decreases with increasing temperature.
(5) Optimization of original COD concentration (influent COD concentration) of wastewater
Based on the organic wastewater with the optimal wastewater salt concentration and COD concentration of 2000mg/L, 4000mg/L, 6000 mg/L, 8000mg/L, 10000 mg/L and 12000mg/L obtained in the step (2), inoculating fermentation liquor (the effective viable bacteria content is not less than 2 multiplied by 109/mL) of a salt-tolerant strain into a 500mL conical flask containing 200mL of organic wastewater according to the optimal inoculation amount obtained in the step (1), adjusting the pH of the organic wastewater to the optimal pH obtained in the step (3), culturing in a constant-temperature shaking box with the optimal treatment temperature and 200 r/min obtained in the step (4), detecting the degradation condition of the wastewater under the condition of the original COD concentration of each wastewater after 24 hours, and drawing a change curve of COD degradation rate along with the change of the original COD concentration, wherein the change curve is shown in figure 6. As can be seen from FIG. 6, the COD removal rate increased with the increase in the influent COD concentration; when the COD concentration of the inlet water is 6000 mg/L, the removal rate of COD reaches 67.5 percent; as the influent COD concentration continues to increase, the COD removal rate decreases with increasing influent COD concentration.
EXAMPLE five
Rhodococcus erythropolis.Application of HQC-1 in wastewater treatment
The beef extract liquid culture medium in the third embodiment is adopted for cultureRhodococcus erythropolis.HQC-1, high salt high COD wastewater: the concentration of sodium sulfate is 60g/L, and the concentration of COD is 7000-12000 mg/L; adjusting the pH value of the high-salt high-COD wastewater to 7, and adjusting the COD concentration of the wastewater to 8000 mg/L; selection of OD according to the cultivation method of example three600Inoculating the cultured bacterial liquid with the volume of 1.0 percent into high-salt high-COD wastewater according to the inoculation amount of 5 percent (V/V), culturing in a constant-temperature shaking box with the temperature of 35 ℃ and the speed of 200 r/min, and detecting the degradation condition of COD after 24 hours, wherein the removal rate of the COD in the wastewater reaches 62 percent.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Sequence listing
<110> Beijing Han Qi environmental technology Co., Ltd
<120> high-salt-resistance high-COD wastewater degrading bacterial strain, culture method, bacterial liquid and application
<130>WZF015PTA01F1910131
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<170>SIPOSequenceListing 1.0
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<213> Rhodococcus erythropolis (Rhodococcus erythropolis.)
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ggcccagact cctacgggag gcagcagtgg ggaatattgc acaatgggcg aaagcctgat 360
gcagcgacgc cgcgtgaggg atgacggcct tcgggttgta aacctctttc agcagggacg 420
aagcgcaagt gacggtacct gcagaagaag caccggctaa ctacgtgcca gcagccgcgg 480
taatacgtag ggtgcaagcg ttgtccggaa ttactgggcg taaagagttc gtaggcggtt 540
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cttgagtact gcaggggaga ctggaattcc tggtgtagcg gtgaaatgcg cagatatcag 660
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gtgggtagcg aacaggatta gataccctgg tagtccacgc cgtaaacggt gggcgctagg 780
tgtgggttcc ttccacggaa tccgtgccgt agctaacgca ttaagcgccc cgcctgggga 840
gtacggccgc aaggctaaaa ctcaaaggaa ttgacggggg cccgcacaag cggcggagca 900
tgtggattaa ttcgatgcaa cgcgaagaac cttacctggg tttgacatat accggaaagc 960
tgcagagatg tggcccccct tgtggtcggt atacaggtgg tgcatggctg tcatcagctc 1020
gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctatctt atgttgccag 1080
cacgttatgg tggggactcg taagagactg ccggggtcaa ctcggaggaa ggtggggacg 1140
acgtcaagtc atcatgcccc ttatgtccag ggcttcacac atgctacaat ggccagtaca 1200
gagggctgcg agaccgtgag gtggaacgaa tcccttaaag ctggtctcag ttcggatcgg 1260
ggtctgcaac tcgaccccgt gaagtcggag tcgctagtaa tcgcagatca gcaacgctgc 1320
ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac gtcatga 1367
Claims (10)
1. A degrading bacterial strain for high-salt-resistant high-COD wastewater is characterized in that: the strain is Rhodococcus erythropolis (A)Rhodococcus erythropolis) Is named asRhodococcus erythropolis.HQC-1, preserved in China general microbiological culture Collection center (CGMCC), with the address: the microbial research institute of western road No. 1, china academy of sciences, north jing, chaoyang district, with the preservation number: 19104 and preserving for the following time: 12 and 6 in 2019.
2. The high-salt and high-COD resistant wastewater degrading bacterial strain of claim 1, which is characterized in that: the bacterial strain shows positive by gram staining, the bacterial colony is round when the bacterial strain is cultured on a flat plate, the surface is smooth and slightly convex, the edge is regular, the color is red, and the bacterial strain is spherical after microscopic examination.
3. The high-salt and high-COD resistant wastewater degrading bacterial strain of claim 1, which is characterized in that: the strain is screened from sludge near a wastewater discharge outlet.
4. The high-salt and high-COD resistant wastewater degrading bacterial strain of claim 1, which is characterized in that:
the screening and domestication of the strain comprises the following steps:
collecting a sludge sample near a wastewater discharge outlet, inoculating the sludge sample on a beef extract liquid culture medium with the sodium sulfate concentration of 20g/L according to the inoculation amount of 2-10%, and carrying out enrichment culture at the temperature of 30 ℃ to obtain an acclimation culture solution;
circularly inoculating the domestication culture solution obtained in the step (1) on a beef extract liquid culture medium with the sodium sulfate concentration increased by 10g/L each time, and circularly domesticating until the salt concentration in the beef extract liquid culture medium reaches 80 g/L;
and separating out the strains in the finally obtained domestication culture solution to obtain the strains of the sulfate-resistant strains.
5. The high-salt and high-COD resistant wastewater degrading bacterial strain of claim 1, which is characterized in that: the range of high salt tolerance of the strain is 40-80 g/L, and the range of high COD tolerance of the strain is 4000-12000 mg/L.
6. The method for culturing a degrading bacterial strain capable of resisting high-salt high-COD wastewater as claimed in any one of claims 1-5, wherein: inoculating the strain into a beef extract liquid culture medium according to the inoculation amount of 2-10%, wherein the components of the culture medium are as follows: 0.1-1.0% of beef extract; peptone 0.1-3.0%; 0.1 to 1.0 percent of sodium chloride; potassium dihydrogen phosphate 0.01-1.0%; magnesium sulfate 0.01-1.0%, adjusting pH to 6.0-8.0, culturing at 20-45 deg.C for 16-32 hr in constant temperature shaking incubator at 20-45 deg.C, and selecting OD600The bacterial liquid of 0.4-1.8 is bacterial liquid of bacterial strain.
7. A bacterial liquid prepared by using the high-salt-resistance high-COD wastewater degrading bacterial strain of any one of claims 1 to 5.
8. The bacterial liquid according to claim 7, wherein: the process for preparing the bacterial liquid comprises the following steps of inoculating the bacterial strain into a beef extract liquid culture medium according to the inoculation amount of 2-10%, culturing for 16-32h in a constant-temperature shaking incubator at the temperature of 20-45 ℃, and selecting OD600The bacterial liquid of 0.4-1.8 is bacterial liquid of bacterial strain.
9. The use of a high salt tolerant high COD wastewater degrading bacterial strain according to any of claims 1 to 5, wherein: the strain is applied to removal of COD in high-salt high-COD wastewater.
10. The use of the high-salt and high-COD resistant wastewater degrading bacterial strain of claim 10, wherein: the strain is added into the high-salt high-COD wastewater according to the inoculation amount of 2-10% to remove COD, the temperature of the strain for removing the COD of the wastewater is 30-40 ℃, and the pH value is 6.0-8.0.
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