CN111117888A

CN111117888A - Variable-speed oscillating device for cell culture

Info

Publication number: CN111117888A
Application number: CN202010048608.7A
Authority: CN
Inventors: 范金婷; 王浩怡
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-01-16
Filing date: 2020-01-16
Publication date: 2020-05-08

Abstract

The invention discloses a variable-speed oscillating device for cell culture, which comprises a machine body, wherein an installation cover is rotatably connected above the machine body, the installation cover is of a hollow structure, a plurality of installation grooves are formed in the machine body, fixed grooves are formed in the installation grooves, a large balancing weight and a small balancing weight are symmetrically and fixedly connected to two sides of the bottom of each fixed groove, a culture medium is clamped on the fixed grooves, a driving mechanism is arranged below each installation groove and comprises a constant-speed motor and a driven gear, and a fixed shaft is fixedly connected below each fixed groove. The constant-speed motor is used for driving the sector gear, the fixed groove is designed to be of a structure with the gravity center deviating from the center, so that the sector gear can continue to rotate under the action of inertia after being separated from the driven gear, the effect of variable-speed rotation of the culture medium is achieved, and the effect of variable-speed culture oscillation can be achieved by using the simple structure.

Description

Variable-speed oscillating device for cell culture

Technical Field

The invention relates to the technical field of biological cell culture equipment, in particular to a variable-speed oscillating device for cell culture.

Background

The shaking culture, also called shaking culture, refers to a culture method in which microbial cells are inoculated in a liquid culture medium and placed on a shaking table or a shaker for continuous shaking, the shaking can make the culture medium fully contact with oxygen, the supply of dissolved oxygen is increased, the thallus after shaking culture is uniformly propagated, the culture efficiency is high, the method is widely applied to strain screening and microorganism expanded culture, and is a common culture method in the fields of microbial physiology, biochemistry, fermentation and other life science researches.

Generally, the shaking table used by shaking the culture medium needs to be designed into a variable-speed structure, the variable-speed circuit design cost is higher, and the variable-speed design is relatively complex to the internal structure design, so that the manufacturing cost of the shaking equipment is higher.

Disclosure of Invention

The invention aims to solve the defects in the prior art, such as: the variable speed motor design is relatively complex, which causes the cost of the oscillation device to be higher, and the proposed variable speed oscillation device for cell culture.

In order to achieve the purpose, the invention adopts the following technical scheme:

a variable-speed oscillation device for cell culture comprises a machine body, wherein an installation cover is rotatably connected above the machine body and is of a hollow structure, a plurality of installation grooves are formed in the machine body, fixing grooves are formed in the installation grooves, a large balancing weight and a small balancing weight are symmetrically and fixedly connected to two sides of the bottom of each fixing groove, a culture medium is clamped on each fixing groove, a driving mechanism is arranged below each installation groove and comprises a constant-speed motor and driven gears, a fixing shaft is fixedly connected to the lower portion of each fixing groove, a plurality of fixing blocks corresponding to the fixing shafts are fixedly installed in the machine body, the fixing shafts are rotatably inserted into the fixing blocks, the constant-speed motor is arranged on one side of the fixing blocks, a sector gear is fixedly connected to the driving end of the constant-speed motor, and a plurality of the driven gears are respectively fixedly sleeved on the fixing, the fixed shaft is meshed with the driven gear.

Preferably, a groove is formed in the fixing block, the fixing shaft is inserted into the groove, a bearing is further sleeved on the outer side of the fixing shaft, and the fixing shaft is rotatably connected with the fixing block through the bearing.

Preferably, the inner wall of the fixing groove is provided with a clamping block, and the culture medium is provided with a clamping groove corresponding to the clamping block.

Preferably, the material of big balancing weight is the metal material, the material of little balancing weight is the plastic material.

Compared with the prior art, the invention has the beneficial effects that: use the uniform velocity motor to drive sector gear, design into the structure of focus skew center with the fixed slot for sector gear can continue to rotate under the inertial action after breaking away from with driven gear, thereby realizes the effect to culture medium variable speed pivoted, utilizes simple structure, just can realize the effect of variable speed cultivation oscillation.

Drawings

FIG. 1 is a schematic structural diagram of a variable speed shaking device for cell culture according to the present invention;

FIG. 2 is a schematic structural diagram of a sector gear of a variable speed oscillating device for cell culture according to the present invention.

In the figure: 1 organism, 2 installation covers, 3 culture medium, 4 big balancing weights, 5 little balancing weights, 6 fixed slots, 7 fixed axles, 8 fixed blocks, 9 driven gears, 10 bearings, 11 uniform motors, 12 sector gears.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

Referring to fig. 1-2, a variable speed oscillation device for cell culture comprises a machine body 1, an installation cover 2 is rotatably connected above the machine body 1, the installation cover 2 is a hollow structure, a plurality of installation grooves are formed in the machine body 1, fixing grooves 6 are formed in the installation grooves, a large balancing weight 4 and a small balancing weight 5 are symmetrically and fixedly connected to two sides of the bottom of each fixing groove 6, a culture medium 3 is clamped on each fixing groove 6, a driving mechanism is arranged below each installation groove and comprises a constant speed motor 11 and driven gears 9, a fixing shaft 7 is fixedly connected below each fixing groove 6, a plurality of fixing blocks 8 corresponding to the fixing shafts 7 are fixedly installed in the machine body 1, the fixing shafts 7 are rotatably inserted into the fixing blocks 8, the constant speed motor 11 is arranged on one side of the fixing blocks 8, a sector gear 12 is fixedly connected to a driving end of the constant speed motor 11, and the driven gears 9 are, the fixed shaft 7 is meshed with the driven gear 9;

wherein, set up flutedly on the fixed block 8, fixed axle 7 is inserted and is established in the recess, bearing 10 has still been cup jointed in the outside of fixed axle 7, fixed axle 7 passes through bearing 10 and is connected with the rotation of fixed block 8, install bearing 10 between the bottom of fixed axle 7 and fixed block 8, bearing 10 makes the rotation between fixed axle 7 and the fixed block 8 be connected, reduce frictional force between the two, make fixed axle 7 can be in the same direction as smooth at the pivoted in-process, be provided with the fixture block on the inner wall of fixed slot 6, set up the draw-in groove that corresponds with the fixture block on the culture medium 3, fixture block and draw-in groove joint, realize the fixed of culture medium 3 in fixed slot 6, the material of big balancing weight 4 is the metal material, the material of.

In the present invention, when the user uses the apparatus, the user opens the installation cover 2, and the culture medium 3 containing cells and culture solution is respectively clamped on each fixing groove 6, then the mounting cover 2 is closed, the mounting cover 2 has the function of isolation protection, the non-working personnel can be prevented from directly touching the culture medium 3 in the process of oscillation, then start each uniform velocity motor 11, the drive end of uniform velocity motor 11 can drive sector gear 12 and rotate, sector gear 12 is sectorial structure, so only part can be with driven gear 9 intermeshing, sector gear 12 can realize with driven gear 9 meshing part that sector gear 12's rotation drives driven gear 9 and rotates, driven gear 9's rotation can drive fixed axle 7 and fixed slot 6 in step and rotate, the rotation of fixed slot 6 can drive culture medium 3 synchronous rotation, the oscillation effect to the cultured cell is realized in the rotation of culture medium 3.

The sector gear 12 of the sector structure rotates within a circle, only the part meshed with the driven gear 9 can realize the synchronous rotation of the driven gear 9 along with the sector gear 12, the part not meshed with the driven gear 9 can idle, the part meshed with the driven gear 9 and the sector gear 12 drives the driven gear 9 to rotate, an initial speed for rotating the fixed groove 6 is given, a bearing 10 is arranged between the fixed shaft 7 and the fixed block 8, the initial speed enables the fixed groove 6 to continue to rotate, wherein the bottom of the fixed groove 6 is provided with a large balancing weight 4 and a small balancing weight 5, the mass of the large balancing weight 4 is different from that of the small balancing weight 5, the gravity centers of the fixed groove 6, the large balancing weight 4 and the small balancing weight 5 are not in the central position, when the sector gear 12 rotates to the position separated from the driven gear 9, the fixed groove 6 can continue to rotate under the inertia effect, the fixed groove 6 with the center of gravity deviated from the center continues to rotate under the action of inertia, the rotating process is a gradual speed reduction process, the speed reduction realizes the effect of variable-speed rotation of the fixed groove 6, the fixed groove 6 freely rotates until the sector gear 12 rotates until the sector gear is meshed with the driven gear 9 again, and then the initial speed of rotation of the fixed groove 6 is given again.

The drive end of the uniform speed motor 11 can only realize the uniform speed rotation of the sector gear 12, but after the rotation of the sector gear 12 drives the driven gear 9, the fixed shaft 7 and the fixed groove 6 to rotate, the fixed groove 6 will do the deceleration rotation motion under the inertia effect, and the inertia effect is utilized to enable the culture medium 3 to continuously rotate in a variable speed manner, thereby achieving the oscillation effect on the cultured cells.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A variable-speed oscillation device for cell culture comprises a machine body (1) and is characterized in that an installation cover (2) is rotatably connected above the machine body (1), the installation cover (2) is of a hollow structure, a plurality of installation grooves are formed in the machine body (1), fixed grooves (6) are formed in the installation grooves, large balancing weights (4) and small balancing weights (5) are symmetrically and fixedly connected to two sides of the bottom of each fixed groove (6), a culture medium (3) is clamped on the fixed grooves (6), a driving mechanism is arranged below each installation groove and comprises a constant-speed motor (11) and a driven gear (9), a fixed shaft (7) is fixedly connected below each fixed groove (6), a plurality of fixed blocks (8) corresponding to the fixed shafts (7) are fixedly installed in the machine body (1), and the fixed shafts (7) are rotatably inserted in the fixed blocks (8), the uniform-speed motor (11) is arranged on one side of the fixed block (8), a sector gear (12) is fixedly connected to the driving end of the uniform-speed motor (11), the driven gears (9) are fixedly sleeved on the fixed shaft (7) respectively, and the fixed shaft (7) is meshed with the driven gears (9).

2. The variable-speed oscillating device for cell culture according to claim 1, wherein the fixing block (8) is provided with a groove, the fixing shaft (7) is inserted into the groove, the outer side of the fixing shaft (7) is further sleeved with a bearing (10), and the fixing shaft (7) is rotatably connected with the fixing block (8) through the bearing (10).

3. The variable-speed shaking device for cell culture according to claim 1, wherein a clamping block is disposed on an inner wall of the fixing groove (6), and a clamping groove corresponding to the clamping block is disposed on the culture medium (3).

4. The variable-speed oscillating device for cell culture according to claim 1, wherein the large weight block (4) is made of metal, and the small weight block (5) is made of plastic.