CN111100825B - Bacillus and application thereof in industry - Google Patents
Bacillus and application thereof in industry Download PDFInfo
- Publication number
- CN111100825B CN111100825B CN202010122178.9A CN202010122178A CN111100825B CN 111100825 B CN111100825 B CN 111100825B CN 202010122178 A CN202010122178 A CN 202010122178A CN 111100825 B CN111100825 B CN 111100825B
- Authority
- CN
- China
- Prior art keywords
- strain
- alginate
- fermentation
- bacillus
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a bacillus and application thereof in industry, the bacillus screens rotten kelp picked from yellow sea, and the kelp is preserved in China center for type culture collection in 2019, 4 and 28 months, with the preservation number: CCTCC No. M2019315, it can produce alginate lyase, and the produced alginate lyase has wide temperature range of 30-45 deg.C. The enzyme activity of the alginate lyase produced by the strain obtained by screening is wide in temperature range, and has certain advantages in industry, a fermentation culture medium specially developed for the strain with the preservation number of CCTCC No. M2019315 is sufficient in nutrition, and the strain can grow at high density, so that the amount of the alginate lyase produced is large, the alginate degradation capability is strong, and the yield of the product alginate oligosaccharide is high.
Description
Technical Field
The invention relates to a bacillus and an application thereof in industry, in particular to a bacillus capable of producing alginate lyase and having wide enzyme activity adaptive temperature range and an application of the strain in industry, namely a brown alginate oligosaccharide prepared by using the strain, and belongs to the technical field of marine organisms.
Background
Algin is a linear acidic polysaccharide composed of two kinds of mono-uronic acid, alpha-L guluronic acid and beta-D mannuronic acid, and is widely present in cell walls of large-scale brown algae plants such as kelp and undaria pinnatifida. Alginate oligosaccharides, degradation products of algin, have a variety of biological activities, such as: reducing blood fat, resisting virus, resisting tumor, resisting oxidation, etc. Currently, there are two methods for producing brown algae oligosaccharides: one-step method and two-step method. The one-step method is to directly complete the fermentation production of the brown algae oligosaccharide in one step. On one hand, the method has the disadvantages that the optimal conditions for enzyme production and enzymolysis are inconsistent, so that the production efficiency is low; on the other hand, part of the brown algae oligosaccharides produced in the fermentation production process can be utilized by the bacteria, resulting in low product content. The two-step method is to prepare alginate lyase and then carry out enzymolysis, thereby overcoming the defects of the one-step method and being the best choice for producing the alginate oligosaccharide at present.
Disclosure of Invention
The first purpose of the invention is to provide a bacillus capable of producing the alginate lyase and having wide temperature range of enzyme activity adaptation of the produced alginate lyase.
The second purpose of the invention is to provide a method for industrially preparing the brown algae oligosaccharide, which has short fermentation period and high product yield by utilizing the bacillus and combining a specific fermentation culture medium.
In order to achieve the above object, the present invention adopts the following technical solutions:
a bacillus strain, which is named by classificationBacillus sp.The bacillus is characterized in that rotten kelp picked from yellow sea is screened and stored in China center for type culture collection in 2019, 4 and 28 months, and the preservation numbers are as follows: CCTCC number M2019315, which can produce alginate lyase with wide temperature range of 30-45 deg.c.
The application of the bacillus with the preservation number of CCTCC number M2019315 in preparing the brown alginate oligosaccharides is characterized in that the method for preparing the brown alginate oligosaccharides by using the bacillus with the preservation number of CCTCC number M2019315 specifically comprises the following steps:
step 1: culturing the activated strain for 24h at 30 ℃ and 200rpm by using a shake flask, then inoculating the bacterial liquid in the shake flask into a primary fermentation tank, and carrying out amplification culture for 24h at 30 ℃ and 200rpm to obtain seed liquid;
step 2: inoculating the seed solution into a secondary fermentation tank, and fermenting the strain to produce enzyme to obtain fermentation liquor;
and step 3: centrifuging the fermentation liquor at 4 ℃ to remove thalli, and obtaining a crude enzyme solution;
and 4, step 4: adding the crude enzyme solution into the alginate solution, and performing enzymolysis at 30-45 deg.C for 22h to obtain enzymolysis solution, i.e. alginate-oligosaccharide solution;
and 5: sequentially carrying out ultrafiltration, nanofiltration and freeze drying on the enzymolysis liquid to obtain powder, namely the brown algae oligosaccharide.
The application is characterized in that in the step 1, the formula of the seed culture medium used for culturing the strain is as follows:
5g/L of glucose, 5g/L of peptone, 10g/L of yeast extract powder and seawater.
The aforementioned application is characterized in that, in step 2, the formulation of the fermentation medium in the fermenter is:
3g/L of glucose, 3g/L of sodium alginate, 3g/L of peptone, 6g/L of yeast extract powder and tap water.
The use as described above, wherein, in step 2, the strain produces the enzyme by fermentation:
adjusting the ventilation of the fermentation tank to 24m in 0-6h3The rotation speed is 140rpm, and the temperature is 30 ℃;
adjusting the ventilation of the fermentation tank to 36m in the 6 th to 20 th hours of fermentation3The rotation speed is 160rpm, and the temperature is 30 ℃;
adjusting the aeration rate of the fermentation tank to 24m in the 20 th to 24 th hours of the fermentation3H, rotation speed 140rpm, temperature 30 ℃.
The aforementioned use is characterized in that, in step 4, the enzymatic hydrolysis temperature is 35 ℃.
The invention has the advantages that:
(1) the strain with the preservation number of CCTCC number M2019315 obtained by breeding can produce the alginate lyase, and the enzyme activity of the produced alginate lyase has wider temperature range and certain advantages in industry;
(2) the fermentation medium specially developed for the strain with the preservation number of CCTCC number M2019315 is very suitable for the growth of the strain, the growth density of the strain can reach the highest value after fermentation for 22-24 hours, the high-density growth of the strain can shorten the fermentation period, and the whole fermentation period only needs 48 hours;
(3) the specially developed fermentation medium contains peptone and yeast extract powder, so that the nutrition is sufficient, and the strain can grow at high density, so that the quantity of the produced alginate lyase is large, the capacity of degrading the alginate is strong, and the yield of the product alginate oligosaccharide is high.
Drawings
FIG. 1 is a phylogenetic tree of strain 22D-12 we constructed;
FIG. 2 is a flow diagram for the preparation of alginate oligosaccharides;
FIG. 3 is a sheet chromatography profile of an enzymatic product;
FIG. 4 is a graph showing the results of the examination of the effect of temperature on the activity of alginate lyase.
Detailed Description
The invention is described in detail below with reference to the figures and the embodiments.
Bacterial strain screening, identification and phylogenetic tree construction
1. Strain screening
Preparing MAlg medium (selective medium): 3g/L of glucose, 3g/L of sodium alginate, 3g/L of peptone, 6g/L of yeast extract powder, 20g/L of agar and tap water.
Preparing a seed culture medium: 5g/L of glucose, 5g/L of peptone, 10g/L of yeast extract powder and seawater.
In 2018, 3 and 20 months, 4 rotten kelps are collected from the yellow sea, the 4 rotten kelps are put together for homogenization, and clear liquid is taken for gradient dilution (10)-1、10-2、10-3、10-4、10-5、10-6、10-7、10-8) Then, the cells were plated on MALg plate medium, and the corresponding plates were designated as 1P, 2P, 3P, 4P, 5P, 6P, 7P and 8P, respectively, and these 8 plates were subjected to inversion culture at 30 ℃ for 24 hours, after which they were subjected to constant temperature inversion culture22 single colonies are picked out from 8 plates, the 22 single colonies are inoculated into 22 seed culture media respectively, culturing at 30 deg.C and 200rpm for 24h, inoculating into 22 new MALg plate culture medium, the 22 new MALg plate culture media are respectively recorded as 1D, 2D, 3D, 1...., 20D, 21D and 22D, and are subjected to inverted culture at the constant temperature of 30 ℃ for 24h again, where a number of distinct degradation circles appeared on the plate labeled 22D, and the colonies on this plate were numbered sequentially (22D-1, 22D-2, 22D-3,..., 22D-15, 22D-16), then, the colony (number 22D-12) in which the degradation cycle was most evident was isolated and purified, and the strain corresponding to the colony was designated as strain 22D-12.
2. Identification of Strain 22D-12
Firstly, extracting DNA of the strain 22D-12 by using a kit extraction method, then obtaining 16S rDNA of the strain 22D-12 through PCR amplification, and performing similarity comparison with a bacterial 16S rDNA sequence in a database by using a Blast program in NCBI.
And (3) comparing the results: strain 22D-12 is Bacillus (B.sub.), (B.sub.), (B.sub.) (B.Bacillus sp.) The strain of (1).
3. Construction of phylogenetic Tree of Strain 22D-12
After obtaining 16S rDNA of the strain 22D-12, a phylogenetic tree of the strain was constructed using MEGA 5.1 software (Neighbor-Joining) (see FIG. 1), and the evolutionary relationship of each strain was analyzed.
And (3) analysis results: strain 22D-12 is Bacillus (B.sub.), (B.sub.), (B.sub.) (B.Bacillus sp.) The strain of (1).
Second, strain preservation
The strain 22D-12 obtained by screening is an alginate lyase enzyme-producing strain due to the most obvious degradation cycle generated on a MALg plate culture medium, and the produced alginate lyase has high enzyme activity, so that the strain 22D-12 is preserved in China center for type culture Collection with the preservation addresses as follows: wuhan, China, the preservation date is: 28 th 4 month in 2019, the preservation number is: CCTCC number M2019315, with classification name: bacillus (Bacillus sp.).
Thirdly, utilizing the strain 22D-12 to industrially prepare the brown algae oligosaccharide
We describe the method for industrially preparing alginate-derived oligosaccharides by using the strain 22D-12 in detail below. Referring to fig. 2, the method specifically includes the following steps:
1. preparation of seed liquid
Seed culture medium: 5g/L of glucose, 5g/L of peptone, 10g/L of yeast extract powder, preparing with seawater, and sterilizing for later use.
Culturing the activated strain 22D-12 for 24h by using a shake flask (containing a seed culture medium) under the conditions of the temperature of 30 ℃ and the rotation speed of 200rpm, then inoculating the bacterial liquid in the shake flask into a 200L fermentation tank containing 50L of seed culture medium by 2 percent of inoculum size, and carrying out amplification culture for 24h under the conditions of the temperature of 30 ℃ and the rotation speed of 200rpm to obtain a seed liquid.
2. Enzyme production by fermentation
Fermentation medium: 3g/L of glucose, 3g/L of sodium alginate, 3g/L of peptone, 6g/L of yeast extract powder and tap water, and sterilizing for later use.
Inoculating the seed solution into a 1000L fermentation tank filled with 500L fermentation medium in an inoculation amount of 10%, so that the strain is fermented to produce enzyme, and obtaining fermentation liquor, wherein:
adjusting the ventilation of the fermentation tank to 24m in 0-6h3The rotation speed is 140rpm, and the temperature is 30 ℃;
adjusting the ventilation of the fermentation tank to 36m in the 6 th to 20 th hours of fermentation3The rotation speed is 160rpm, and the temperature is 30 ℃;
adjusting the aeration rate of the fermentation tank to 24m in the 20 th to 24 th hours of the fermentation3H, rotation speed 140rpm, temperature 30 ℃.
3. Preparation of crude enzyme solution
The fermentation broth was centrifuged at 8000rpm at 4 ℃ for 10min to remove the bacteria, and a crude enzyme solution was obtained.
4. Enzymolysis reaction
Preparing a substrate: dissolving sodium alginate to prepare sodium alginate solution with the concentration of 20 g/L.
Adding the crude enzyme solution into 1000L sodium alginate solution (the amount of sodium alginate is 20 kg) at a volume ratio of 1:9, and performing enzymolysis at 35 deg.C for 22h to obtain enzymolysis solution, i.e. brown algae oligosaccharide solution.
5. Separating and purifying enzymolysis product
The enzymolysis liquid is sequentially subjected to ultrafiltration, nanofiltration and freeze drying to obtain 13.34kg of powder, namely the brown algae oligosaccharide, and the yield of the product is 66.7 percent by calculation.
Fourthly, qualitative analysis is carried out on the enzymolysis products
The Thin Layer Chromatography (TLC) is used for carrying out preliminary qualitative analysis on the alginate-derived oligosaccharides as the enzymolysis product.
A Silica Gel 60F 254 plate (German merck) is adopted, the sample loading amount is 2 mu L, a developing agent system is n-butyl alcohol, formic acid and water = 4: 5: 1 (V: V), the Silica Gel plate is placed in a chromatographic cylinder, a chromatographic solution is developed twice, air drying is carried out at room temperature after each development, 10% sulfuric acid-ethanol solution is used as a color developing agent, after air drying is carried out again, thin plate chromatography is carried out on an enzymolysis product.
After the enzymolysis product is subjected to thin plate chromatography, the chromatography is shown in fig. 3, wherein the left side is the alginate oligosaccharide standard sample, and the standard sample sequentially comprises the following components from top to bottom: fucoidan, and fucoidan; degradation products are on the right.
As shown in FIG. 3, the degradation products obtained by degrading algin with the strain 22D-12 contain three fucoidan oligosaccharides, fucoidan trisaccharide, fucoidan tetrasaccharide and fucoidan hexasaccharide.
Fifthly, the optimum temperature of the enzymolysis reaction
The algin lyase forms an unsaturated double bond between a non-reducing end C4 and a C5 through a beta elimination mechanism, the unsaturated double bond has strong absorption at 235nm, and the absorbance value and the amount of the formed unsaturated double bond form a linear relationship in a certain range.
Adding 0.1ml of the crude enzyme solution into 0.9ml of alginate solution, keeping the temperature at 40 ℃ for 10min, then putting the solution in a water bath at 100 ℃ to inactivate the enzyme for 5min to terminate the enzymolysis reaction, and measuring the light absorption value of the reaction system at 235nm by taking the inactivated enzyme as a contrast.
Definition of enzyme activity units: under the above conditions, the amount of enzyme that increases the absorbance by 0.1 per min was one enzyme activity unit (EU).
Mixing the crude enzyme solution with phosphate buffer solution, incubating at different temperatures (30 deg.C, 35 deg.C, 40 deg.C, 45 deg.C, 50 deg.C, 60 deg.C) for 10min, and measuring enzyme activity by ultraviolet spectrophotometry. The highest point of enzyme activity is defined as relative enzyme activity of 100%.
The results of the enzyme activity measurement are shown in FIG. 4.
As can be seen from FIG. 4, the alginate lyase has a wide temperature range, the relative enzyme activity is above 75% between 30 ℃ and 45 ℃, and the optimal enzyme reaction temperature is 35 ℃.
Therefore, the algin lyase produced by the strain 22D-12 obtained by screening has a wider temperature range, and the strain 22D-12 has certain advantages in industry.
It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the protection scope of the present invention.
Claims (6)
1. A bacillus strain, which is named by classificationBacillus sp.The bacillus is characterized in that rotten kelp picked from yellow sea is screened and stored in China center for type culture collection in 2019, 4 and 28 months, and the preservation numbers are as follows: CCTCC number M2019315, which produces alginate lyase with wide temperature range of enzyme activity, relative enzyme activity of over 75% at 30-45 deg.c and optimal enzyme reaction temperature of 35 deg.c.
2. The method for preparing brown algae oligosaccharide by using the bacillus with the preservation number of CCTCC number M2019315 as claimed in claim 1, is characterized by comprising the following steps:
step 1: culturing the activated strain for 24h at 30 ℃ and 200rpm by using a shake flask, then inoculating the bacterial liquid in the shake flask into a primary fermentation tank, and carrying out amplification culture for 24h at 30 ℃ and 200rpm to obtain seed liquid;
step 2: inoculating the seed solution into a secondary fermentation tank, and fermenting the strain to produce enzyme to obtain fermentation liquor;
and step 3: centrifuging the fermentation liquor at 4 ℃ to remove thalli, and obtaining a crude enzyme solution;
and 4, step 4: adding the crude enzyme solution into the alginate solution, and performing enzymolysis at 30-45 deg.C for 22h to obtain enzymolysis solution, i.e. alginate-oligosaccharide solution;
and 5: sequentially carrying out ultrafiltration, nanofiltration and freeze drying on the enzymolysis liquid to obtain powder, namely the brown algae oligosaccharide.
3. The method according to claim 2, wherein in step 1, the formulation of the seed medium used for culturing the strain is:
5g/L of glucose, 5g/L of peptone, 10g/L of yeast extract powder and seawater.
4. The method of claim 3, wherein in step 2, the fermentation medium in the fermentor is formulated as:
3g/L of glucose, 3g/L of sodium alginate, 3g/L of peptone, 6g/L of yeast extract powder and tap water.
5. The method according to claim 2, wherein in step 2, when the strain ferments to produce the enzyme:
adjusting the ventilation of the fermentation tank to 24m in 0-6h3The rotation speed is 140rpm, and the temperature is 30 ℃;
adjusting the ventilation of the fermentation tank to 36m in the 6 th to 20 th hours of fermentation3The rotation speed is 160rpm, and the temperature is 30 ℃;
adjusting the aeration rate of the fermentation tank to 24m in the 20 th to 24 th hours of the fermentation3H, rotation speed 140rpm, temperature 30 ℃.
6. The method of claim 2, wherein in step 4, the enzymatic hydrolysis temperature is 35 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010122178.9A CN111100825B (en) | 2020-02-27 | 2020-02-27 | Bacillus and application thereof in industry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010122178.9A CN111100825B (en) | 2020-02-27 | 2020-02-27 | Bacillus and application thereof in industry |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111100825A CN111100825A (en) | 2020-05-05 |
| CN111100825B true CN111100825B (en) | 2021-05-11 |
Family
ID=70427803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010122178.9A Active CN111100825B (en) | 2020-02-27 | 2020-02-27 | Bacillus and application thereof in industry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111100825B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113278549A (en) * | 2021-05-19 | 2021-08-20 | 广西大学 | Bacillus cereus and application thereof |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733118B (en) * | 2020-08-17 | 2020-12-04 | 中国科学院烟台海岸带研究所 | Bacillus PL-2 and application thereof in aquaculture |
| CN112694995B (en) * | 2021-01-11 | 2022-07-26 | 山东省科学院生物研究所 | Algin degrading bacterium Bacillus HZ-1 and application thereof in holothurian culture |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586150A (en) * | 2012-03-03 | 2012-07-18 | 浙江大学舟山海洋研究中心 | Bacterial strain capable of generating alginate lyase and fermentation method thereof |
| CN103695341A (en) * | 2013-12-16 | 2014-04-02 | 江南大学 | Alginate lyase secreted from marine bacterium and preparation method thereof |
| CN104195080A (en) * | 2014-08-23 | 2014-12-10 | 中国科学院天津工业生物技术研究所 | Bacillus sp capable of producing alginate lyase and application thereof |
| CN108277184A (en) * | 2018-03-30 | 2018-07-13 | 中国科学院烟台海岸带研究所 | Produce the bacillus and its preparation method and application of algin catenase |
| CN108929859A (en) * | 2018-08-03 | 2018-12-04 | 中国热带农业科学院热带生物技术研究所 | One type bacterial strain of bacillus HB172198 and its application |
| CN110387367A (en) * | 2019-09-03 | 2019-10-29 | 中国海洋大学 | A kind of novel algin catenase |
| CN110438111A (en) * | 2019-07-24 | 2019-11-12 | 山东海之宝海洋科技有限公司 | A kind of algin catenase and its application |
-
2020
- 2020-02-27 CN CN202010122178.9A patent/CN111100825B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102586150A (en) * | 2012-03-03 | 2012-07-18 | 浙江大学舟山海洋研究中心 | Bacterial strain capable of generating alginate lyase and fermentation method thereof |
| CN103695341A (en) * | 2013-12-16 | 2014-04-02 | 江南大学 | Alginate lyase secreted from marine bacterium and preparation method thereof |
| CN104195080A (en) * | 2014-08-23 | 2014-12-10 | 中国科学院天津工业生物技术研究所 | Bacillus sp capable of producing alginate lyase and application thereof |
| CN108277184A (en) * | 2018-03-30 | 2018-07-13 | 中国科学院烟台海岸带研究所 | Produce the bacillus and its preparation method and application of algin catenase |
| CN108929859A (en) * | 2018-08-03 | 2018-12-04 | 中国热带农业科学院热带生物技术研究所 | One type bacterial strain of bacillus HB172198 and its application |
| CN110438111A (en) * | 2019-07-24 | 2019-11-12 | 山东海之宝海洋科技有限公司 | A kind of algin catenase and its application |
| CN110387367A (en) * | 2019-09-03 | 2019-10-29 | 中国海洋大学 | A kind of novel algin catenase |
Non-Patent Citations (2)
| Title |
|---|
| 利用芽孢杆菌发酵生产褐藻胶裂解酶的研究;李悦明等;《中国酿造》;20101231(第4期);79-81 * |
| 海藻工具酶——褐藻胶裂解酶研究进展;李丽妍等;《生物工程学报》;20110625;第27卷(第6期);838-845 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113278549A (en) * | 2021-05-19 | 2021-08-20 | 广西大学 | Bacillus cereus and application thereof |
| CN113278549B (en) * | 2021-05-19 | 2022-07-05 | 广西大学 | Bacillus cereus and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111100825A (en) | 2020-05-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111100825B (en) | Bacillus and application thereof in industry | |
| CN113186121B (en) | Caproic acid bacteria capable of utilizing various substrates and application thereof | |
| CN102533605B (en) | Strain capable of producing levansucrase and method for producing levan by using levansucrase | |
| CN111100827B (en) | Bacillus capable of producing high-activity alginate lyase and application thereof | |
| CN111484954B (en) | Pseudomonas nigricans for producing alginate lyase | |
| CN108753642B (en) | Flavobacterium johnsonii strain for producing alginate lyase | |
| CN109943617A (en) | The method for producing butyric acid and/or its esters | |
| CN117229979B (en) | Extended microbubble strain for producing algin lyase and application thereof | |
| CN111826308B (en) | Marine sediment-derived chitin efficient degrading bacterium and application thereof | |
| CN111548959B (en) | Klebsiella pneumoniae and application thereof | |
| CN104342372B (en) | Method for producing yeast autolysate by probiotic fermentation | |
| CN113930368A (en) | Bacillus vallismortis and application thereof in vinegar brewing | |
| CN111019995B (en) | Method for producing vanillin by fermentation with eugenol as substrate | |
| CN106754486B (en) | Pseudomonas for high-yield trehalose synthase and fermentation enzyme production method thereof | |
| CN111100829B (en) | Bacterial strain capable of degrading specific fragment brown algae polysaccharide | |
| CN113151061B (en) | Glucose-inhibited oxytoca | |
| CN112442453B (en) | Ormokodak yeast for degrading biogenic amine and application of same in white spirit brewing | |
| CN112094762B (en) | Corynebacteria vinifera strain and application thereof | |
| CN111100826B (en) | Lexella capable of producing alginate lyase and application thereof | |
| CN110592047B (en) | Novel method for producing feruloyl esterase by fermenting straws with Verbena pyricularis and application | |
| CN113801815A (en) | Strain for producing beta-mannase and application thereof | |
| CN106754829A (en) | A kind of method of utilization bacillus HS17 fermenting and producing chitosan enzymes and its application | |
| JP2004344084A (en) | Alcohol fermentative yeast | |
| CN114437985B (en) | Enterobacter aerogenes and application thereof in synthesis of microbial polysaccharide | |
| CN113621518B (en) | Method for breeding spore bacteria for alcohol decomposition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |