CN111096197A

CN111096197A - Preparation and application of edible fungus medium-temperature sterilization synergist

Info

Publication number: CN111096197A
Application number: CN201910997294.2A
Authority: CN
Inventors: 关良洲; 张英君; 展张洋
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-10-05
Filing date: 2019-10-05
Publication date: 2020-05-05

Abstract

The preparation and application of the edible fungus medium temperature sterilization synergist are characterized in that: the edible fungus medium-temperature sterilization synergist comprises the following biological agents, by weight, 20-30 parts of natamycin, 25-35 parts of polylysine hydrochloride, 25-35 parts of nisin, 10-20 parts of sodium diacetate and application of the edible fungus medium-temperature sterilization synergist, wherein the edible fungus medium-temperature sterilization synergist is uniformly mixed into a culture material required by edible fungus production according to the proportion of 0.20-0.25% of dry materials, a proper amount of water is added for fully stirring, then the mixture is bagged and sterilized in time, the sterilization is maintained for 6 hours when the effective sterilization temperature reaches 95 ℃ during normal-pressure sterilization, the stewing is carried out for 8 hours, the sterilization process is finished, the effective temperature is maintained for 2 hours when the high-pressure sterilization is carried out, the stewing is carried out for 4 hours, the sterilization process is finished, and the energy consumption and two thirds of labor are saved compared with the traditional sterilization.

Description

Preparation and application of edible fungus medium-temperature sterilization synergist

The technical field is as follows:

the invention belongs to a new technical formula and application of an edible fungus sterilization method in the agricultural industry.

Technical background:

in the planting production of the edible fungus industry at present, the most important technical link in the production process of strains and cultivation bags is sterilization, the sterilization method is mainly divided into normal pressure sterilization and high pressure sterilization, the normal pressure sterilization is maintained for 15-20 hours under the condition that the temperature reaches 100 ℃ (actually only reaches 95-97 ℃), then the sterilization is carried out for 8-10 hours, and some time is even longer, the high pressure sterilization is to place a fungus rod into an autoclave, the temperature reaches 115 ℃ and 120 ℃ for 8-10 hours, then the sterilization is carried out for 4-6 hours, and the longer the sterilization process is, the more fuel and labor are consumed.

Particularly, in the process of sterilization and temperature rise, when the material temperature is in the middle temperature range of 30-50 ℃, a large number of microorganisms such as bacteria and yeasts propagate to cause acidification of culture materials and consumption of nutrient substances, so that the yield and quality of the produced edible fungus bags at the later stage can be influenced, the edible fungus middle-temperature sterilization synergist is added into the culture materials, the propagation of the microorganisms such as the bacteria and the yeasts can be inhibited when the sterilization reaches the middle temperature range, when the temperature of the culture materials is increased to 97-100 ℃, the physical and chemical aspects act together to quickly kill all fungi, bacteria and even bacterial spores. Has no toxic and side effects.

The preparation and application of the edible fungus medium-temperature sterilization synergist can reduce the sterilization time by two thirds, save fuel, reduce labor in the sterilization process and realize energy conservation and emission reduction.

The invention content is as follows:

the invention is that a mixed inhibitor (edible fungus medium temperature sterilization synergist) which can inhibit the growth of microbial bacteria and other miscellaneous fungi in edible fungus culture medium is added, the edible fungus medium temperature sterilization synergist selects biological agents such as natamycin, polylysine hydrochloride, nisin and sodium diacetate which are uniformly mixed in the edible fungus culture medium during mixing, the edible fungus medium temperature sterilization synergist is used for controlling the material temperature to be in a medium temperature range of 30-50 ℃, a large number of microorganisms such as bacteria and saccharomycetes are propagated, the acidification of the culture medium and the consumption of nutrient substances are prevented, four raw materials selected for preparing the edible fungus medium temperature sterilizing agent are all safe substances which are recognized at home and abroad and can be applied to food and medicines, the natamycin, the polylysine hydrochloride and the nisin belong to biological agents, the natamycin is a natural, broad-spectrum, efficient and safe filamentous fungus inhibitor such as saccharomycetes, mycete and the like, it can not only inhibit fungi, but also prevent the generation of mycotoxin, the national ministry of health of 1997 officially approves natamycin as food preservative, polylysine hydrochloride is a homomorphic monomer polymer containing 25-35L-amino acid residues from streptomyces albus controlled fermentation culture solution through ion exchange resin adsorption, desorption and purification, has obvious inhibition effect on gram-positive and gram-negative bacteria, saccharomycete, mould, virus and the like, can be converted into lysine through decomposition and conversion of edible fungus mycelium in the later period, provides nutrient source for edible fungi, nisin is a polypeptide consisting of 34 amino acids, can inhibit the growth and reproduction of heat-resistant bacillus, ensures that the complete sterilization effect is achieved under the condition of moderate temperature, sodium diacetate mainly interferes the interaction of enzyme through effectively permeating into the cell wall of the mould, the sodium diacetate has particularly obvious inhibition effect on aspergillus niger, rhizopus nigricans, aspergillus flavus and trichoderma viride.

The preparation of the edible fungus medium temperature sterilization synergist comprises the following components in parts by weight: 25% of natamycin, 30% of polylysine hydrochloride, 30% of nisin and 15% of sodium diacetate.

The Chinese food additive Standard chemical technology Committee formally approved natamycin as a food preservative in 1996. The FAO/WHO combined food additive expert committee stipulates that a human body can unconditionally accept the daily intake of 0.3mg of natamycin per kilogram of body weight, the national standard GB2760 stipulates the application range and the dosage of the natamycin, and the national health family planning Committee No. 5 bulletin about the approval of new 4 food additive varieties such as epsilon-polylysine and the like in 2014 4, 3, 2014 approves polylysine hydrochloride as a new food additive variety. In 1969, the food and agriculture organization/world health organization (FAL/WHO) food additives joint experts Committee of the United nations confirmed that nisin could act as a food preservative.

The document approved by the department of health in the middle of 3 months 1992 states: "Streptococcus lactis can be scientifically considered as safe as a food preservative". The national food safety standard GB 25538-2010 food additive sodium diacetate and other national standards can prove that the raw materials in the formula of the medium-temperature sterilization synergist for the edible fungi conform to the food safety, and the Chinese sterilization synergist for the edible fungi is not directly added into the edible fungi product, but is added into a culture material for planting the edible fungi, and the addition amount is very small, so that the food safety problem does not exist.

The medium temperature sterilization synergist for edible fungi is applied by uniformly mixing the medium temperature sterilization synergist of the edible fungi into culture materials required by edible fungi production according to the proportion of 0.20-0.25% of dry materials, adding a proper amount of water, fully stirring, then bagging, sterilizing in time after bagging, maintaining for 6 hours when the sterilization effective temperature reaches 95 ℃ during normal pressure sterilization, stewing for 8 hours, finishing the sterilization process, maintaining for 2 hours when the sterilization effective temperature reaches 115 ℃ during high pressure sterilization, stewing for 4 hours, and finishing the sterilization process.

The specific implementation mode is as follows:

The medium temperature sterilization synergist for edible fungi is applied by uniformly mixing the medium temperature sterilization synergist of the edible fungi into culture materials required by edible fungi production according to the proportion of 0.20-0.25% of dry materials, adding a proper amount of water, fully stirring, then bagging, sterilizing in time after bagging, maintaining for 6 hours when the sterilization effective temperature reaches 95 ℃ during normal pressure sterilization, stewing for 8 hours, finishing the sterilization process, maintaining for 1 hour when the sterilization effective temperature reaches 115 ℃ during high pressure sterilization, stewing for 4 hours, and finishing the sterilization process.

Claims

1. The preparation of the edible fungus medium temperature sterilization synergist is characterized in that: the composition comprises the following biological agents, by weight, 20-30 parts of natamycin, 25-35 parts of polylysine hydrochloride, 25-35 parts of nisin and 10-20 parts of sodium diacetate.

2. The medium temperature sterilization synergist for edible fungi is applied by uniformly mixing the medium temperature sterilization synergist of the edible fungi into culture materials required by edible fungi production according to the proportion of 0.20-0.25% of dry materials, adding a proper amount of water, fully stirring, then bagging, sterilizing in time after bagging, maintaining for 6 hours when the sterilization effective temperature reaches 95 ℃ during normal pressure sterilization, stewing for 8 hours, finishing the sterilization process, maintaining for 1 hour when the sterilization effective temperature reaches 115 ℃ during high pressure sterilization, stewing for 4 hours, and finishing the sterilization process.