CN111088165A - Microorganism asexual propagation fermentation method and device - Google Patents
Microorganism asexual propagation fermentation method and device Download PDFInfo
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- CN111088165A CN111088165A CN201811240309.2A CN201811240309A CN111088165A CN 111088165 A CN111088165 A CN 111088165A CN 201811240309 A CN201811240309 A CN 201811240309A CN 111088165 A CN111088165 A CN 111088165A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 58
- 230000004151 fermentation Effects 0.000 title claims abstract description 58
- 244000005700 microbiome Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 29
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 81
- 239000004576 sand Substances 0.000 claims abstract description 29
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 26
- 238000003756 stirring Methods 0.000 claims abstract description 22
- 230000000813 microbial effect Effects 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 238000010008 shearing Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 98
- 239000007788 liquid Substances 0.000 claims description 45
- 239000011229 interlayer Substances 0.000 claims description 37
- 230000005540 biological transmission Effects 0.000 claims description 17
- 230000007246 mechanism Effects 0.000 claims description 17
- 230000001105 regulatory effect Effects 0.000 claims description 16
- 238000010438 heat treatment Methods 0.000 claims description 15
- 238000007599 discharging Methods 0.000 claims description 12
- 238000005086 pumping Methods 0.000 claims description 12
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- 244000068988 Glycine max Species 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 150000001720 carbohydrates Chemical class 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 2
- 125000000185 sucrose group Chemical group 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 description 13
- 239000001301 oxygen Substances 0.000 description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 108010066057 cabin-1 Proteins 0.000 description 10
- 239000012071 phase Substances 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000010563 solid-state fermentation Methods 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 229960004793 sucrose Drugs 0.000 description 3
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 3
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 230000004103 aerobic respiration Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
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- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- Chemical & Material Sciences (AREA)
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Abstract
The invention provides a microbial asexual propagation fermentation method and a device. The asexual propagation and fermentation method of the microorganisms is characterized in that in the step of pressurizing, dispersing and homogenizing, the microorganisms and a culture medium are added into a cabin body for mixing and stirring, silica sand is added for stirring, microbial colonies are removed through shearing force between the silica sand and the silica sand, the microorganisms return to the logarithmic phase, and the microorganisms do not need to pass through a spore phase again, so that the effect of accelerating the fermentation speed of the microorganisms is achieved.
Description
Technical Field
The invention relates to a microbial asexual propagation fermentation method and a device thereof, in particular to a method capable of accelerating the microbial propagation fermentation speed.
Background
Common wine, fruit vinegar, etc. are obtained by fermentation with microorganisms, wherein the fermentation can be divided into solid fermentation and liquid fermentation. Solid state fermentation generally refers to a biological reaction process with one or more microorganisms in a solid substrate of a certain humidity in the almost absence of free water. Liquid fermentation refers to a biological reaction process with a liquid phase as a continuous phase.
Since the solid fermentation is performed for a long time and the productivity is limited, the solid fermentation is not suitable for use in a large-scale production process. As for liquid fermentation, since fermentation time is short and quality control is easy, it is a form suitable for producing products with high efficiency.
Although the fermentation efficiency of liquid fermentation is higher than that of solid fermentation, the microorganisms must complete fermentation through the life cycle of N spore phases, logarithmic phase, stationary phase and decay phase in the fermentation process, so the required fermentation time cannot be effectively shortened.
Disclosure of Invention
The invention mainly aims to provide a microbial asexual propagation fermentation method and a microbial asexual propagation fermentation device, which can accelerate the fermentation speed of microorganisms and further shorten the manufacturing time of food.
The purpose and the effect of the invention are realized by the following technologies:
a method for vegetative propagation and fermentation of microbes includes such steps as adding the liquid mixture containing silica sand, culture medium, saccharide and microbes to a cabin, heating the liquid mixture in the cabin in water-proof mode, stirring while regulating speed, and removing the microbial colony by the shearing force between silica sand and silica sand.
The method for asexual propagation and fermentation of microorganisms as described above, wherein the weight ratio (wt%) of silica sand, medium and saccharide is 1:3 to 5:3 to 24.
The method for asexual propagation and fermentation of microorganisms as described above, wherein the medium is soybean powder.
The method for asexual propagation and fermentation of microorganisms as described above, wherein the sugar is sucrose.
The method for asexual propagation and fermentation of microorganisms as described above, wherein the mixed liquid in the cabin is heated to 37 ℃ in a water-proof manner.
The asexual propagation and fermentation method of microorganisms, wherein the speed of the speed-adjusting stirring is 300 rpm.
A microorganism asexual propagation fermentation device comprises: a cabin, a speed regulating motor, a stirring mechanism, a high-pressure air and water liquid whirling mechanism, a heating unit and a water pumping motor; wherein:
the peripheral wall of the lower section of the cabin body is annularly provided with a gap interlayer, the upper end and the bottom end of the gap interlayer are respectively provided with a water inlet and a water outlet, water is injected into the gap interlayer from the water inlet, and the water in the gap interlayer is discharged from the water outlet; the top end face of the cabin body is provided with a vent valve capable of adjusting the pressure in the cabin body, the cabin body is provided with a feeding hole, an upper discharging hole and a lower discharging hole, raw materials are fed through the feeding hole, the upper discharging hole is used for collecting aged fermentation liquor, and the lower discharging hole is used for taking out silica sand, culture medium and microorganisms;
the speed regulating motor is arranged on the outer side of the cabin body and is provided with a power output end;
the stirring mechanism comprises a transmission shaft and blades, the transmission shaft is connected with the power output end of the speed regulating motor, and a plurality of groups of blades are arranged on the transmission shaft in different height groups;
the high-pressure air and water liquid backflow mechanism comprises a venturi and a backflow pipe, the venturi is a three-fork manifold with two inlets and one outlet, high-pressure air is input at a first input end of the venturi, the second input end of the venturi is an outlet end connected with the backflow pipe, the output end of the venturi is connected with the upper end of the cabin body, the inlet end of the backflow pipe is connected with the lower end of the cabin body, and mixed liquid in the cabin body can enter the cabin body again through the venturi from the backflow pipe;
the heating unit is arranged corresponding to the gap interlayer and is used for heating the water liquid in the gap interlayer;
the water pumping motor is provided with a water inlet end and a water outlet end, the water inlet end is connected with the lower part of the gap interlayer, the water outlet end is connected with the higher part of the gap interlayer, the water pumping motor is started, water in the gap interlayer is pumped out from the water inlet end, and then the water is introduced into the gap interlayer from the water outlet end.
The microorganism asexual propagation fermentation device as described above, wherein the radius of the blade arranged at the lowest position on the transmission shaft is the largest.
The microorganism asexual propagation fermentation device as described above, wherein the inlet end of the whirling flow pipe is connected to the lower end of the cabin, and the installation position of the whirling flow pipe is relatively higher than the installation position of the lowest blade.
The invention has the advantages that:
the invention atomizes the water liquid which flows back into the cabin again by inputting high-pressure air to be matched, so as to refine water molecules and improve oxygenation efficiency, thereby being beneficial to the reproduction of microorganisms.
In addition, the invention also further removes the bacterial colony of the microorganism through the shearing force generated between the silica sand and the silica sand in the stirring process, so that the microorganism directly returns to the logarithmic phase without passing through the spore phase, and the effect of shortening the fermentation time is achieved.
Drawings
FIG. 1 is a flow chart of the microbial asexual propagation fermentation method of the present invention.
FIG. 2 is a schematic view showing the structure of a microorganism asexual propagation fermentation apparatus according to the present invention.
FIG. 3 is a schematic view of the microorganism asexual propagation fermentation apparatus of the present invention in a use operation state.
Fig. 4 is a partially enlarged view of fig. 3.
The main reference numbers illustrate:
the method comprises the following steps of 1, a cabin body, 11 gap isolation layers, 111 water inlets, 112 water outlets, 12 vent valves, 13 material inlet holes, 14 material outlet holes, 15 material outlet holes, 2 speed regulation motors, 3 stirring mechanisms, 31 transmission shafts, 32 blades, 4 high-pressure air and water liquid circulation mechanisms, 41 venturi tubes, 411 first input ends, 412 second input ends, 413 output ends, 42 circulation tubes, 421 output ends, 422 inlet ends, 5 heating units, 6 water pumping motors, 61 water inlet ends, 62 water outlet ends, S1 step one, S2 step two, S3 step three and S4 step four.
Detailed Description
For a more complete and clear disclosure of the technical content, objects and advantages achieved by the present invention, reference is now made to the following detailed description, taken in conjunction with the accompanying drawings and in which:
referring to fig. 1 and 2, a fermentation apparatus capable of performing asexual propagation of microorganisms is disclosed, which comprises: a cabin body 1, a speed regulating motor 2, a stirring mechanism 3, a high-pressure air and water liquid backflow mechanism 4, a heating unit 5 and a water pumping motor 6; wherein:
the peripheral wall of the lower section of the cabin body 1 is provided with a gap interlayer 11, the upper end and the bottom end corresponding to the gap interlayer 11 are respectively provided with a water inlet 111 and a water outlet 112, water is injected into the gap interlayer 11 from the water inlet 111, and the water in the gap interlayer 11 is discharged from the water outlet 112; the top end face of the cabin body 1 is provided with a vent valve 12 which can adjust the cabin pressure in the cabin body 1, the cabin body 1 is provided with a feeding hole 13, an upper discharging hole 14 and a lower discharging hole 15, the feeding hole 13 is used for feeding raw materials, the upper discharging hole 14 is used for collecting mature fermentation liquor, and the lower discharging hole 15 is used for taking out silica sand, culture medium and microorganism.
The speed regulating motor 2 is arranged above the outer side of the cabin body 1 and is provided with a power output end.
The transmission shaft 31 of the stirring mechanism 3 is connected with the power output end of the speed regulating motor 2, so that the speed regulating motor drives the transmission shaft 31 to rotate, a plurality of groups of blades 32 which are arranged in groups with different heights are arranged on the transmission shaft 31 in series, and the radius of the blade 32 at the bottom is the largest.
The high-pressure air and water liquid circulating mechanism 4 comprises a venturi tube 41 and a circulating pipe 42, the venturi tube is a three-fork manifold with two inlets and one outlet, the high-pressure air is input at a first input end 411 of the venturi tube 41, the second input end 412 is an outlet end 421 connected with the circulating pipe 42, an output end 413 of the venturi tube 41 is connected with the upper end of the cabin body 1, an inlet end 422 of the circulating pipe 42 is connected with the lower end of the cabin body 1, so that the mixed liquid in the cabin body 1 enters the circulating pipe 42 from the inlet end 422, and then enters the venturi tube 41 from the outlet end 421 of the circulating pipe 42 through the second input end 412 of the venturi tube 41.
The heating unit 5 is arranged corresponding to the gap interlayer 11 of the cabin body 1, heats the water liquid of the gap interlayer 11, and further heats the contents in the cabin body 1 in a water-proof manner.
The water pumping motor 6 is provided with a water inlet end 61 and a water outlet end 62, the water inlet end 61 is connected with the lower part of the gap interlayer 11, the water outlet end 62 is connected with the higher part of the gap interlayer 11, water in the gap interlayer 11 is pumped out from the water inlet end 61 by starting the water pumping motor 6 and then is led into the gap interlayer 11 from the water outlet end 62, and the circulation is carried out, so that the water temperature in the gap interlayer 11 can be uniformly kept in a set temperature range.
When the asexual propagation fermentation of microorganisms is started by the equipment (please refer to fig. 3 and fig. 4), firstly, the silica sand, the culture medium and the saccharides are put into the cabin body 1 according to the weight ratio (wt%) of 1: 3-5: 3-24, and the microorganisms are put into the cabin body 1 through the material inlet 13, water is added to ensure that the water level is flush with the blade 32 at the top of the transmission shaft 31 to form mixed liquid, meanwhile, the water is injected into the gap interlayer 11 through the water inlet 111, the heating unit 5 starts to heat to insulate the mixed liquid in the cabin body 1, the speed regulating motor 2 is started to default temperature, and the speed regulating motor drives the blade 32 to stir the mixed liquid through the transmission shaft 31 of the stirring mechanism 3; the water in the gap interlayer 11 is pumped out by the water pumping motor 6 from the water inlet end 61 and then is led into the gap interlayer 11 from the water outlet end 62, and the circulation is performed in such a way, so that the water temperature in the gap interlayer 11 can be uniformly kept in a set temperature range; meanwhile, the mixed liquid at the bottom of the cabin 1 is led out from the inlet end 422 by the venturi principle to enter the return pipe 42 and enter the venturi 41 from the outlet end 421 of the return pipe 42, and the high-pressure air input from the first input end 411 of the venturi 41 of the high-pressure air and water liquid return mechanism 4 accelerates the mixed liquid entering the venturi 41 to be sent into the cabin 1 through the output end 413 of the venturi 41, so that the returned mixed liquid enters the cabin 1 to be atomized instantly and combined with oxygen to increase the oxygen content in water molecules, and the propagation speed of microorganisms is accelerated through the action of the water molecules with high oxygen content and the microorganisms, particularly through the stirring disturbance of the blades 32, a strong shearing force channel is generated between silica sand and silica sand, the colony of the microorganisms is removed through the shearing force, so that the microorganisms directly return to the logarithmic growth phase without returning to the spore phase, to shorten the fermentation time of the microorganisms.
Namely, a microorganism asexual propagation fermentation method (refer to fig. 1 and 3) is performed by the above-mentioned apparatus, and the method comprises the following steps:
step S1, adding a mixed liquid containing silica sand, a culture medium, saccharides and microorganisms into a cabin 1, wherein the weight ratio (wt%) of the silica sand, the culture medium and the saccharides is 1: 3-5: 3-24;
step two S2, heating the mixed liquid in the cabin body 1 in a waterproof way, simultaneously stirring the mixed liquid at a speed regulation way, and removing the microbial colonies by utilizing the shearing force between the silica sand and the silica sand through stirring, so that the microorganisms return to the logarithmic phase without passing through the spore phase;
step three S3, draining the mixed liquid at the bottom of the cabin body 1;
step S4, the recirculated mixed liquid is introduced into the cabin 1 by high pressure air, so that when entering the cabin 1, water mist is formed and oxygen is mixed in to form high oxygen micro-molecular water, thereby improving the high oxygen environment and facilitating the rapid proliferation of aerobic respiratory microorganisms.
The following is further illustrated with actual operational data:
examples
10 kg of silica sand, 30 kg of soybean powder and 30 kg of cane sugar.
Description of the operation:
1. 10 kg of silica sand is washed and then added into the cabin body 1, 30 kg of soybean powder and 30 kg of cane sugar are placed into the cabin body 1, then water is added into the cabin body 1, the water level is enabled to be flush with the blades 32 on the upper section of the transmission shaft 31, and then strains are added.
2. The gap interlayer 11 is filled with clean water to about 9 minutes.
3. The heating unit 5 and the water pumping motor 6 are started to heat the clear water in the gap interlayer 11 by the heating unit 5, and then the mixed liquid in the cabin 1 is heated up to 37 ℃ in a water-proof way and kept, and meanwhile, the mixed liquid in the cabin 1 is led out to the high-pressure air and water liquid reflux mechanism 4 and is sent into the cabin 1 again.
4. The speed regulating motor 2 is started and the rotating speed reaches 300rpm, and the speed regulating motor 2 drives the blades 32 to rotate through the transmission shaft 31 to stir the mixed liquid.
5. The bacterial strain is attached to the soybean powder to start fermentation and gradually form bacterial colonies, the silicon sand, the soybean powder and the bacterial strain are stirred by stirring through the blade 32 at the lower section of the transmission shaft 31, and the bacterial colonies gathered by the bacterial strain are sheared to return to the logarithmic phase by utilizing the shearing force between the silicon sand and the silicon sand, so that the effect of rapid fermentation is achieved.
6. Meanwhile, the pure high-pressure air input through the venturi 41 jets the mixed liquid introduced into the venturi 41 through the return pipe 42 into the cabin 1, the high-pressure air atomizes the water, the water can be mixed with oxygen under the atomizing condition, the oxygen content in the water can quickly reach more than 20ppm, and the water can be prevented from aggregating, so that the high-oxygen small molecular water is formed.
7. After the fermentation is finished and the stirring is stopped, because the soybean powder has a specific gravity larger than that of water and is precipitated at the bottom of the chamber body 1, as for the derivative of the strain, the derivative is mostly dissolved in the water and floats in the upper water, the mature fermentation liquid is collected by the upper discharge hole 14, and the silica sand, the soybean powder and the strain are taken out from the lower discharge hole 15.
Comparative example
Taking wine preparation as an example, the traditional solid state fermentation generally needs about one month for wine maturation, but the method of the invention only needs three days, the maturation liquid can reach 14-degree alcohol concentration, the distilled spirit has the alcohol head of 66 degrees, the spirit core of 45 degrees and the spirit tail of 35 degrees, 120 liters of spirit can be distilled from one ton of fermentation liquid, and the alcohol degree is about 52 degrees; the liquor prepared by the method of the invention contains high unsaturated fatty acid of about 1900ppm, the liquor prepared by the traditional solid state fermentation contains only unsaturated fatty acid of about 900ppm, and the unsaturated fatty acid and alcohol can form esters, namely the source of bouquet, under the low temperature condition, so the liquor prepared by the method of the invention has better quality than the liquor prepared by the traditional solid state fermentation.
The method creates a microenvironment with an average value, and the microorganisms are positioned in the positive pressure high oxygen chamber, so that the outside bad bacteria cannot be infected inwards; in addition, the high oxygen environment enables the fungus group which carries out aerobic respiration to rapidly proliferate, the mitochondria-containing strain can carry out aerobic respiration to generate more ATP, and the ATP can easily form saponin and terpenes under the high oxygen environment, so the fermentation liquor can create more valuable derivatives.
In addition, the supply of high-pressure oxygen can be closed, so that the mixed liquid forms anaerobic conditions, and the oxygen-like and anaerobic microbial flora can be controlled to grow and grow in the cabin body according to the length of the supplied air.
Claims (10)
1. A method for asexual propagation and fermentation of microorganisms includes such steps as adding the liquid mixture containing silica sand, culture medium, saccharide and microbes into a cabin, heating the liquid mixture in the cabin in water-proof mode, stirring while regulating speed, and removing the microbial colony by the shearing force between silica sand and silica sand.
2. The method of claim 1, wherein the weight ratio of silica sand, culture medium and saccharide is 1: 3-5: 3-24.
3. The microbial asexual propagation and fermentation method according to claim 2, wherein the medium is soybean powder.
4. The microbial asexual propagation and fermentation method according to claim 3, wherein the sugar is sucrose.
5. The microbial asexual propagation fermentation method according to claim 4, wherein the mixed liquid in the tank is heated to 37 ℃ against water.
6. The microbial asexual propagation fermentation method according to claim 5, wherein the speed of the speed-adjusting stirring is 300 rpm.
7. A microorganism asexual propagation fermentation device comprises: a cabin, a speed regulating motor, a stirring mechanism, a high-pressure air and water liquid whirling mechanism, a heating unit and a water pumping motor; wherein:
the peripheral wall of the lower section of the cabin body is annularly provided with a gap interlayer, the upper end and the bottom end of the gap interlayer are respectively provided with a water inlet and a water outlet, water is injected into the gap interlayer from the water inlet, and the water in the gap interlayer is discharged from the water outlet; the top end face of the cabin body is provided with a vent valve capable of adjusting the pressure in the cabin body, the cabin body is provided with a feeding hole, an upper discharging hole and a lower discharging hole, raw materials are fed through the feeding hole, the upper discharging hole is used for collecting aged fermentation liquor, and the lower discharging hole is used for taking out silica sand, culture medium and microorganisms;
the speed regulating motor is arranged on the outer side of the cabin body and is provided with a power output end;
the stirring mechanism comprises a transmission shaft and blades, the transmission shaft is connected with the power output end of the speed regulating motor, and a plurality of groups of blades are arranged on the transmission shaft in different height groups;
the high-pressure air and water liquid backflow mechanism comprises a venturi and a backflow pipe, the venturi is a three-fork manifold with two inlets and one outlet, high-pressure air is input at a first input end of the venturi, the second input end of the venturi is an outlet end connected with the backflow pipe, the output end of the venturi is connected with the upper end of the cabin body, the inlet end of the backflow pipe is connected with the lower end of the cabin body, and mixed liquid in the cabin body can enter the cabin body again through the venturi from the backflow pipe;
the heating unit is arranged corresponding to the gap interlayer and is used for heating the water liquid in the gap interlayer;
the water pumping motor is provided with a water inlet end and a water outlet end, the water inlet end is connected with the lower part of the gap interlayer, the water outlet end is connected with the higher part of the gap interlayer, the water pumping motor is started, water in the gap interlayer is pumped out from the water inlet end, and then the water is introduced into the gap interlayer from the water outlet end.
8. The microbial asexual propagation fermentation device according to claim 7, wherein the radius of the blade arranged at the lowest position on the transmission shaft is the largest.
9. The apparatus according to claim 8, wherein the inlet end of the convection tube is connected to the lower end of the chamber and is located at a position higher than the lowest position of the blades.
10. The apparatus according to claim 7, wherein the inlet end of the convection tube is connected to the lower end of the chamber and is located at a position higher than the lowest position of the blades.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811240309.2A CN111088165A (en) | 2018-10-24 | 2018-10-24 | Microorganism asexual propagation fermentation method and device |
Applications Claiming Priority (1)
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| US20090098619A1 (en) * | 2007-10-16 | 2009-04-16 | Shiu Nan Chen | Method of producing liquid and powered mushroom beta-glucan |
| CN104560716A (en) * | 2013-10-15 | 2015-04-29 | 吕胜一 | Logarithmic proliferation fermentation method and device |
| CN104651156A (en) * | 2015-03-10 | 2015-05-27 | 袁青 | Logarithmic proliferous fermentation method and equipment |
| CN209508291U (en) * | 2018-10-24 | 2019-10-18 | 孔东宁 | Microorganism vegetative propagation installation for fermenting |
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| JPS60164478A (en) * | 1984-02-08 | 1985-08-27 | Ajinomoto Co Inc | Method for cultivating mold |
| US4625001A (en) * | 1984-09-25 | 1986-11-25 | Nippon Shokubai Kagaku Kogyo Co., Ltd. | Method for continuous production of cross-linked polymer |
| US20090098619A1 (en) * | 2007-10-16 | 2009-04-16 | Shiu Nan Chen | Method of producing liquid and powered mushroom beta-glucan |
| CN104560716A (en) * | 2013-10-15 | 2015-04-29 | 吕胜一 | Logarithmic proliferation fermentation method and device |
| CN104651156A (en) * | 2015-03-10 | 2015-05-27 | 袁青 | Logarithmic proliferous fermentation method and equipment |
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