CN111088150A - Culture medium nitrogen filling method - Google Patents
Culture medium nitrogen filling method Download PDFInfo
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- CN111088150A CN111088150A CN201911394106.3A CN201911394106A CN111088150A CN 111088150 A CN111088150 A CN 111088150A CN 201911394106 A CN201911394106 A CN 201911394106A CN 111088150 A CN111088150 A CN 111088150A
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- needle head
- nitrogen
- nitrogen filling
- culture medium
- exhaust
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 179
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 90
- 239000001963 growth medium Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 13
- 229920001971 elastomer Polymers 0.000 claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 9
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 5
- 229930182555 Penicillin Natural products 0.000 claims abstract description 4
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims abstract description 4
- 229940049954 penicillin Drugs 0.000 claims abstract description 4
- 239000007789 gas Substances 0.000 claims description 3
- CWERGRDVMFNCDR-UHFFFAOYSA-M thioglycolate(1-) Chemical compound [O-]C(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-M 0.000 abstract description 4
- 238000005429 filling process Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 1
- 229940071127 thioglycolate Drugs 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000033116 oxidation-reduction process Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a culture medium nitrogen filling method, which is used for filling nitrogen and sealing after a thioglycolate fluid culture medium is filled into a penicillin bottle, and comprises three steps of bottle arranging, nitrogen filling and sealing, wherein a nitrogen filling needle head and an exhaust needle head are arranged side by side and clamped on one side of a rubber plug during nitrogen filling, the lower end of the exhaust needle head is lower than the lower end of the nitrogen filling needle head, the nitrogen filling needle head is flush with the lower surface of the rubber plug, the nitrogen filling needle head and the exhaust needle head are quickly and vertically pulled out during sealing, and an aluminum cover is added to finish the whole nitrogen filling process.
Description
Technical Field
The invention belongs to the field of culture medium processing, and particularly relates to a nitrogen filling method for a bottled liquid culture medium.
Background
The thioglycollate culture medium is rich in nutrient components, sodium thioglycollate and glucose are added as reducing agents to absorb oxygen in the culture medium, a lower oxidation-reduction potential is provided for anaerobic bacteria growth, and the thioglycollate serving as an oxidation-reduction indicator can indicate the oxygen-containing degree (light red in the presence of oxygen and colorless in the absence of oxygen) in the culture medium so as to facilitate sterile detection. The thioglycollate medium is mainly used for the sterility test of medicines or foods and can also be used for the culture of pathogenic microorganisms. Because of its comprehensive nutrient components, it can culture most of aerobe and anaerobe, and its preparation is simple, so that it can be extensively used in pharmaceutical industry, medical department and scientific research institute. However, the components of the culture medium are very easy to oxidize, and a pink oxidation layer is easy to appear on the upper layer of the culture medium liquid in the preparation process, so that the effect of the aseptic examination is influenced. At present, the common practice at home and abroad is to boil the culture medium to eliminate an oxide layer before inoculation, but the method consumes energy and labor, and nutrient components are destroyed after the culture medium is subjected to high-temperature treatment again, so that the authenticity and the accuracy of sterility inspection or pathogenic bacteria culture can be directly influenced.
Disclosure of Invention
In order to solve the problems of the existing products and technologies, the invention provides a culture medium nitrogen charging method, which comprises the following steps:
s1, bottle placing: placing the penicillin bottles on an ultra-clean workbench in order;
s2, filling nitrogen: the nitrogen filling needle head and the exhaust needle head are arranged side by side and clamped at one side of the rubber plug, the rubber plug is plugged into the rubber plug by one third of the depth, wherein the lower end of the exhaust needle head is lower than the lower end of the nitrogen filling needle head, and the nitrogen filling needle head is flush with the lower surface of the rubber plug, and nitrogen filling is carried out for 2-5 s;
s3, sealing: after the nitrogen filling is finished, the exhaust needle head, the nitrogen filling needle head and the rubber plug are simultaneously and quickly drawn out upwards and vertically, and the aluminum cover is covered.
Preferably, the nitrogen filling needle and the exhaust needle are fixed side by side, and the distance between the lower ends of the nitrogen filling needle and the exhaust needle is 2-5cm, so that the relative positions of the two needles are ensured, and nitrogen filling and exhaust are facilitated.
Preferably, the outer ring of the upper surface of the rubber plug is provided with an inward recess, so that the exhaust needle head and the nitrogen filling needle head can be conveniently pulled out.
The invention also designs a culture medium nitrogen charging device based on the culture medium nitrogen charging method, and the structure of the culture medium nitrogen charging device comprises: a nitrogen filling needle head, an exhaust needle head, a baffle plate and a nitrogen supply device; the nitrogen filling needle head and the exhaust needle head penetrate through one side edge of the baffle plate, two transverse screw holes are formed in the baffle plate, and the nitrogen filling needle head and the exhaust needle head can be fixed to the side edge of the baffle plate through bolts; the upper end of the nitrogen filling needle head is connected with a nitrogen supply device, and the upper end of the exhaust needle head is communicated with the air.
Preferably, the distance between the nitrogen filling needle and the lower end of the exhaust needle is 2-5 cm.
Preferably, the outer surface of the lower part of the nitrogen filling needle head is provided with length scales.
Preferably, the outer surface of the lower part of the exhaust needle head is provided with length scales.
Preferably, the nitrogen supply device comprises a nitrogen storage device and a gas valve.
The processing method of the invention has simple and rapid nitrogen filling process, good nitrogen filling and sealing effects, and during nitrogen filling, the nitrogen filling needle head is connected with the nitrogen supply device, the upper surface of the rubber plug is clung to the side surface of the lower surface of the baffle plate and clung to the nitrogen filling needle head and the exhaust needle head, the rubber plug is plugged into one third of the depth, the positions of the nitrogen filling needle head and the exhaust needle head are adjusted, the lower end of the nitrogen filling needle head is slightly lower than the lower surface of the rubber plug, the lower end of the exhaust needle head is lower than the lower end of the nitrogen filling needle head and slightly higher than the liquid level of the culture medium, during sealing, the nitrogen filling needle head and the exhaust needle head are quickly and vertically pulled out, and the sealing can be completed by adding the aluminum cover, so.
Drawings
FIG. 1 is a schematic structural diagram of a culture medium nitrogen charging device.
Detailed Description
Example 1
The culture medium nitrogen filling method comprises the following steps:
s1, bottle placing: placing the penicillin bottles on an ultra-clean workbench in order;
s2, filling nitrogen: the nitrogen filling needle head and the exhaust needle head are arranged side by side and clamped at one side of the rubber plug, the rubber plug is plugged into the rubber plug by one third of the depth, wherein the lower end of the exhaust needle head is lower than the lower end of the nitrogen filling needle head, and the nitrogen filling needle head is flush with the lower surface of the rubber plug, and nitrogen filling is carried out for 2 s;
s3, sealing: after the nitrogen filling is finished, the exhaust needle head, the nitrogen filling needle head and the rubber plug are simultaneously and quickly drawn out upwards and vertically, and the aluminum cover is covered.
More specifically, the nitrogen filling needle and the exhaust needle are fixed side by side, and the distance between the lower ends of the nitrogen filling needle and the exhaust needle is 2-5 cm.
More specifically, the outer ring of the upper surface of the rubber plug is provided with an inward recess, and the recess shape of the inward recess is the same as that of the nitrogen filling needle and the exhaust needle which are arranged side by side.
Example 2
As shown in FIG. 1, the culture medium nitrogen charging device of the invention comprises: a nitrogen filling needle head 1, an exhaust needle head 2, a baffle plate 3 and a nitrogen supply device; the nitrogen filling needle head 1 and the exhaust needle head 2 penetrate through one side edge of the baffle plate 3, two transverse screw holes are formed in the baffle plate 3, and the nitrogen filling needle head 1 and the exhaust needle head 2 can be respectively fixed on the side edge of the baffle plate 3 through bolts 4; the upper end of the nitrogen filling needle head 1 is connected with a nitrogen supply device, and the upper end of the exhaust needle head 2 is communicated with the air.
More specifically, the distance between the nitrogen filling needle 1 and the lower end of the exhaust needle 2 is 2-5 cm.
More specifically, the outer surface of the lower part of the nitrogen filling needle head 1 is provided with length scales.
More specifically, the outer surface of the lower part of the exhaust needle head 2 is provided with length scales.
More specifically, the nitrogen supply device comprises a nitrogen storage device 5 and a gas valve 6.
More specifically, the baffle is made of polyurethane material, and the bolt is a plastic bolt.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A culture medium nitrogen charging method is characterized in that: the method comprises the following steps:
s1, bottle placing: placing the penicillin bottles on an ultra-clean workbench in order;
s2, filling nitrogen: the nitrogen filling needle head and the exhaust needle head are arranged side by side and clamped at one side of the rubber plug, the rubber plug is plugged into the rubber plug by one third of the depth, wherein the lower end of the exhaust needle head is lower than the lower end of the nitrogen filling needle head, and the nitrogen filling needle head is flush with the lower surface of the rubber plug, and nitrogen filling is carried out for 2-5 s;
s3, sealing: after the nitrogen filling is finished, the exhaust needle head, the nitrogen filling needle head and the rubber plug are simultaneously and quickly drawn out upwards and vertically, and the aluminum cover is covered.
2. The method for nitrogen-charging culture medium according to claim 1, characterized in that: the nitrogen filling needle head and the exhaust needle head are fixed side by side, and the distance between the lower ends of the nitrogen filling needle head and the exhaust needle head is 2-5 cm.
3. The method for nitrogen-charging culture medium according to claim 1, characterized in that: the outer ring of the upper surface of the rubber plug is provided with an inward recess.
4. A culture medium nitrogen-charging device based on the culture medium nitrogen-charging method of claim 1, characterized in that: the structure includes: a nitrogen filling needle head, an exhaust needle head, a baffle plate and a nitrogen supply device; the nitrogen filling needle head and the exhaust needle head penetrate through one side edge of the baffle plate, two transverse screw holes are formed in the baffle plate, and the nitrogen filling needle head and the exhaust needle head can be fixed to the side edge of the baffle plate through bolts; the upper end of the nitrogen filling needle head is connected with a nitrogen supply device, and the upper end of the exhaust needle head is communicated with the air.
5. The culture medium nitrogen-charging device according to claim 4, characterized in that: the distance between the nitrogen filling needle and the lower end of the exhaust needle is 2-5 cm.
6. The culture medium nitrogen-charging device according to claim 5, characterized in that: the outer surface of the lower part of the nitrogen filling needle head is provided with length scales.
7. The culture medium nitrogen-charging device according to claim 6, characterized in that: the outer surface of the lower part of the exhaust needle head is provided with length scales.
8. The culture medium nitrogen-charging device according to claim 7, characterized in that: the nitrogen supply device comprises a nitrogen storage device and a gas valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911394106.3A CN111088150A (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen filling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911394106.3A CN111088150A (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen filling method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111088150A true CN111088150A (en) | 2020-05-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911394106.3A Pending CN111088150A (en) | 2019-12-30 | 2019-12-30 | Culture medium nitrogen filling method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3950227A (en) * | 1970-12-21 | 1976-04-13 | St. John's University | Batch method of establishing and maintaining a controlled aerobic environment for a microbial culture |
| GB8303096D0 (en) * | 1983-02-04 | 1983-03-09 | Oxoid Ltd | Bacterial testing |
| CN205152210U (en) * | 2015-11-27 | 2016-04-13 | 胡雪琦 | Sulphur glycollate culture medium nitrogen charging arrangement |
| CN206799632U (en) * | 2017-06-12 | 2017-12-26 | 毛瑞婷 | A kind of microbial detection device |
| CN211853522U (en) * | 2019-12-30 | 2020-11-03 | 江苏益玛生物科技有限公司 | Culture medium nitrogen filling needle |
| CN211847925U (en) * | 2019-12-30 | 2020-11-03 | 江苏益玛生物科技有限公司 | Culture medium nitrogen charging device |
-
2019
- 2019-12-30 CN CN201911394106.3A patent/CN111088150A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3950227A (en) * | 1970-12-21 | 1976-04-13 | St. John's University | Batch method of establishing and maintaining a controlled aerobic environment for a microbial culture |
| GB8303096D0 (en) * | 1983-02-04 | 1983-03-09 | Oxoid Ltd | Bacterial testing |
| CN205152210U (en) * | 2015-11-27 | 2016-04-13 | 胡雪琦 | Sulphur glycollate culture medium nitrogen charging arrangement |
| CN206799632U (en) * | 2017-06-12 | 2017-12-26 | 毛瑞婷 | A kind of microbial detection device |
| CN211853522U (en) * | 2019-12-30 | 2020-11-03 | 江苏益玛生物科技有限公司 | Culture medium nitrogen filling needle |
| CN211847925U (en) * | 2019-12-30 | 2020-11-03 | 江苏益玛生物科技有限公司 | Culture medium nitrogen charging device |
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