CN111076787B - Measuring device and measuring method for measuring food intake of bees - Google Patents
Measuring device and measuring method for measuring food intake of bees Download PDFInfo
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- CN111076787B CN111076787B CN201911360326.4A CN201911360326A CN111076787B CN 111076787 B CN111076787 B CN 111076787B CN 201911360326 A CN201911360326 A CN 201911360326A CN 111076787 B CN111076787 B CN 111076787B
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- 241000257303 Hymenoptera Species 0.000 title claims abstract description 34
- 235000012631 food intake Nutrition 0.000 title claims abstract description 34
- 230000037406 food intake Effects 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims description 11
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 235000015097 nutrients Nutrition 0.000 claims abstract description 40
- 230000009471 action Effects 0.000 claims abstract description 4
- 239000011358 absorbing material Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims 1
- 239000006188 syrup Substances 0.000 claims 1
- 235000020357 syrup Nutrition 0.000 claims 1
- 238000005259 measurement Methods 0.000 abstract description 9
- 238000000691 measurement method Methods 0.000 abstract description 4
- 241000256844 Apis mellifera Species 0.000 description 11
- 238000009423 ventilation Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000007605 air drying Methods 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F23/00—Indicating or measuring liquid level or level of fluent solid material, e.g. indicating in terms of volume or indicating by means of an alarm
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- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- General Physics & Mathematics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to the technical field of experimental measurement devices, and discloses a measurement device and a measurement method for measuring food intake of bees, wherein the measurement device comprises a cup body, a cup cover, a conical head, a capillary tube and a nutrient solution bottle; the cup cover is detachably connected with the cup body, the cup cover is provided with a vent groove, the vent groove is provided with a plurality of vent holes, the cup cover is provided with at least one conical through hole, the small diameter end of the conical through hole faces the inner cavity of the cup body, the conical head is arranged in the conical through hole, a gap is arranged between the conical head and the conical through hole, and a capillary tube is clamped and fixed through the adjustment gap; extending the capillary tube into the nutrient solution bottle, and sucking the nutrient solution by utilizing siphon action; taking the capillary vertically from the nutrient solution bottle, and recording the liquid level height by using marks when the liquid level of the capillary is stable; the capillary is vertically inserted into the tapered through hole, fixed by using the tapered head, cultured for a prescribed time, and vertically taken out, and the food consumption of bees is calculated based on the difference between the front and rear of the liquid in the capillary measured.
Description
Technical Field
The invention relates to the technical field of experimental measurement devices, in particular to a measurement device and a measurement method for measuring food intake of bees.
Background
In some experiments, more accurate measurement of bee food intake is needed, but the prior art does not have a measuring device for measuring bee intake, and the measuring device is formed by a beaker and the like, so that the measuring error is large, and more accurate experimental data cannot be obtained.
Accordingly, there is a need in the art for a measuring device and measuring method for measuring bee food intake to solve the above problems.
Disclosure of Invention
In order to solve the problems that the measuring device for measuring the bee intake is not available in the prior art, and the measuring device is formed by a beaker and the like, the measuring error of the method is large, and more accurate experimental data cannot be obtained, the invention provides a measuring device for measuring the bee food intake, which comprises a cup body, a cup cover, a conical head, a capillary tube and a nutrient solution bottle; comprises a cup body, a cup cover, a conical head, a capillary tube and a nutrient solution bottle; the cup cover is detachably connected with the cup body, the cup cover is provided with a vent groove, the vent groove is provided with a plurality of vent holes, the cup cover is provided with at least one conical through hole, the small diameter end of the conical through hole faces the inner cavity of the cup body, the conical head is arranged in the conical through hole, a gap is arranged between the conical head and the conical through hole, and a capillary tube is clamped and fixed through the adjustment gap; extending the capillary tube into the nutrient solution bottle, and sucking the nutrient solution by utilizing siphon action; taking the capillary vertically from the nutrient solution bottle, and recording the liquid level height by using marks when the liquid level of the capillary is stable; the capillary is vertically inserted into the tapered through hole, fixed by using the tapered head, cultured for a prescribed time, and vertically taken out, and the food consumption of bees is calculated based on the difference between the front and rear of the liquid in the capillary measured.
In a preferred embodiment of the above measuring device for measuring food intake of bees, the nutrient solution is sugar water.
In the above preferred embodiment of the measuring device for measuring food intake of bees, the inside of the ventilation groove is provided with a water-absorbing material stained with pure water, so that the air in the cup body is kept moist.
In a preferred embodiment of the above measuring device for measuring food intake of bees, the water absorbing material is cotton.
In the preferred embodiment of the measuring device for measuring food intake of bees, the tube section of the capillary tube extending into the cup body is perpendicular to the bottom of the cup body.
In the above preferred technical solution of the measuring device for measuring bee food intake, scales are provided on the side wall of the nutrient solution bottle.
In a preferred embodiment of the above measuring device for measuring food intake of bees, the capillary has an inner diameter of 0.3 mm to 0.5 mm.
In another aspect, the present invention also provides a measuring method applied to a measuring device for measuring food intake of bees, comprising the steps of:
S1, measuring the liquid level height of a nutrient solution bottle to be a first liquid level value;
s2, placing bees in the cup body, covering the cover body, and feeding, wherein the liquid level of the nutrient solution is a second liquid level value;
s3, obtaining the liquid level height variation according to the difference value between the first liquid level value and the second liquid level value.
In a preferred embodiment of the above measurement method, step S3 further comprises calculating the total mass of food intake of bees according to the level height variation value.
As can be appreciated by those skilled in the art, in the preferred technical scheme of the measuring device for measuring the food intake of bees, as the measuring device for measuring the food intake of bees is not more accurate in the prior art, the experiment is carried out through temporary splicing, no special experimental equipment is available, the mixed use of the experimental equipment can be infected with bacteria and the like, the bees which are raised possibly die, the obtained experimental result is inaccurate, the liquid level change of the nutrient solution bottle can be intuitively observed through the measuring device, secondary measurement is not needed, errors are reduced, and the measuring result is more accurate; through setting up water-absorbing material in the ventilation groove, make the air in the cup keep suitable humidity, reduce because of the air drying leads to the honeybee too much drinking water and leads to the inaccurate influence of experimental result, and then improve the accuracy of experiment. The measuring device can be used once or used for multiple times after disinfection.
Drawings
Preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:
FIG. 1 is a schematic structural view of an embodiment of a measuring device for measuring food intake of bees of the present invention;
FIG. 2 is a top view of the cover of the present invention;
FIG. 3 is a schematic illustration of a capillary marker of the present invention;
FIG. 4 is a flow chart of the measurement method for measuring bee food intake of the present invention;
reference numerals: 1. a cup body; 2. a cup cover; 21. a tapered through hole; 22. a vent groove; 3. a conical head; 4. capillary tube.
Detailed Description
Other advantages and advantages of the present invention will become apparent to those skilled in the art from the following detailed description, which, by way of illustration, is to be read in connection with certain specific embodiments, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, in the description of the present invention, terms such as "medium," "upper," "lower," "inner," and the like indicate directions or positional relationships based on the directions or positional relationships shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the apparatus or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected" and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; may be a mechanical connection; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to the specific circumstances.
The measuring device for measuring the intake of the bees is not available in the prior art based on the background technology, the measuring device is formed by a beaker and the like, the measuring error of the method is large, the problem that more accurate experimental data cannot be obtained is solved, the liquid level change of the nutrient solution bottle can be intuitively observed through the measuring device for the intake of the bees, secondary measurement is not needed, the error is reduced, and the measuring result is more accurate; through setting up water-absorbing material in the ventilation groove, make the air in the cup keep suitable humidity, reduce because of the air drying leads to the honeybee too much drinking water and leads to the inaccurate influence of experimental result, and then improve experimental measurement result's accuracy.
Specifically, as shown in fig. 1 to 3, the measuring device for bee food intake of the invention comprises a cup body 1, a cup cover 2, a conical head 3, a capillary tube 4 and a nutrient solution bottle; comprises a cup body 1, a cup cover 2, a conical head 3, a capillary 4 and a nutrient solution bottle; the cup cover 2 is detachably connected with the cup body 1, the cup cover 2 is provided with a ventilation groove 22, the ventilation groove 22 is provided with a plurality of ventilation holes, the cup cover 2 is at least provided with a conical through hole 21, the small diameter end of the conical through hole 21 faces the inner cavity of the cup body 1, the conical head 3 is arranged in the conical through hole 21, a gap is formed between the conical head 3 and the conical through hole 21, and the capillary 4 is clamped and fixed through the adjustment gap; extending the capillary tube 4 into the nutrient solution bottle, and sucking the nutrient solution by utilizing siphon action; after a certain time, the capillary tube 4 is vertically taken out from the nutrient solution bottle, and when the liquid level of the capillary tube 4 is stable, the height of the liquid level is recorded by a mark; the capillary 4 is vertically inserted into the tapered through hole 21, fixed with the tapered head 3, cultured for a predetermined time, and the capillary 4 is vertically taken out, and the food consumption of bees is calculated from the difference between the front and rear of the liquid in the capillary 4 measured.
In the invention, the cup body 1 and the cup cover 2 are connected in a detachable mode, and the detachable mode can be buckle connection or thread connection; if a buckle connection mode is adopted, a first buckle is arranged on the inner side wall of the cup cover 2, a second buckle is arranged on the outer side wall of the cup body 1, the cup body 1 and the cup cover 2 are detachably connected through the first buckle and the second buckle, and the first buckle and the second buckle adopt a clamping hole structure in the prior art; if a threaded connection mode is adopted, an external thread is arranged on the outer side wall of the cup body 1, an internal thread is arranged on the inner side wall of the cup cover 2, and the cup body 1 and the cup cover 2 are detachably connected through the internal thread and the external thread. Of course, other detachable connection modes can be adopted between the cup body 1 and the cover body. The vent slot 22 may be circular, square, or any other shape, as long as the vent slot 22 has a porous structure, and any changes in the shape of the vent slot 22 by those skilled in the art should be limited within the scope of the present invention.
Preferably, the nutrient solution is sugar water, and the sugar water concentration can be adjusted according to experimental requirements.
Preferably, the air vent groove 22 is provided with a water-absorbing material (not shown) which is adhered with pure water, so that the air in the cup body 1 can be kept at a proper humidity to meet the requirement of experiments on the air humidity, the water-absorbing material can be cotton, gauze or the like, and the type of the water-absorbing material can be changed by a person skilled in the art, and the water-absorbing material is limited in the protection scope of the invention.
Preferably, the water-absorbing material is cotton, has strong water absorption and low cost, and meets the experimental requirements.
Preferably, as shown in fig. 1, the pipe section of the capillary tube 4 extending into the cup body 1 is perpendicular to the bottom of the cup body 1, and the nutrient solution directly flows to the bottom of the cup body 1 through the capillary tube 4, so that the flow resistance of the capillary tube 4 is increased due to the fact that the capillary tube 4 is excessively bent, and the stable flow of the nutrient solution is affected.
Preferably, a scale (not shown in the figure) is arranged on the side wall of the nutrient solution bottle, and the change of the liquid level value is reflected from the scale, so that the change of the liquid level is convenient to check.
Preferably, the inner diameter of the capillary 4 is 0.3 mm to 0.5mm, the intake of bees is not higher than the inner diameter range, which can meet the experimental requirements, and if the inner diameter of the capillary 4 is too large, the cup body 1 can be excessively fed, so that the experimental purpose cannot be achieved.
On the other hand, as shown in fig. 4, the present invention also provides a measuring method applied to a measuring device for measuring food intake of bees, comprising the steps of:
S1, measuring the liquid level height of a nutrient solution bottle to be a first liquid level value;
s2, after bees are placed in the cup body 1 and the cover body is covered for feeding, the liquid level of the nutrient solution is a second liquid level value;
s3, obtaining the liquid level height variation according to the difference value between the first liquid level value and the second liquid level value.
Preferably, step S3 further comprises calculating the total mass of bee food intake from the level height variation value.
The experimental process is as follows: placing a certain number (generally about 20) of bees into the cup body 1, covering the cover body, fixing the length of the capillary tube 4 into the cup body 1 by adjusting the gap between the conical hole and the conical head 3, enabling the part of the tube section of the first end of the capillary tube 4 entering the cup body 1 to be perpendicular to the cup body 1, connecting the second end of the capillary tube 4 with a nutrient solution bottle, enabling the nutrient solution to enter the cup body 1 through the capillary tube 4 for bees to drink, recording the liquid level height in the nutrient solution bottle as a first liquid level value h 1 after the measuring device is connected, placing the measuring device into a constant temperature box, feeding for a period of time (generally 3 hours) under constant temperature conditions, recording the liquid level height in the nutrient solution bottle as a second liquid level value h 2 for achieving a better experimental effect, calculating the difference between the first liquid level value h 1 and the second liquid level value h 2, obtaining the total ingested sugar mass of bees according to the concentration of the nutrient solution, and obtaining an average ingested sugar mass of bees according to the number of bees.
Thus far, the technical solution of the present invention has been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of protection of the present invention is not limited to these specific embodiments. Equivalent modifications and substitutions for related technical features may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and substitutions will be within the scope of the present invention.
Claims (7)
1. The measuring device for measuring the food intake of bees is characterized by comprising a cup body, a cup cover, a conical head, a capillary tube and a nutrient solution bottle;
The cup cover is detachably connected with the cup body, the cup cover is provided with a vent groove, the vent groove is provided with a plurality of vent holes, the cup cover is provided with at least one conical through hole, the small diameter end of the conical through hole faces the inner cavity of the cup body, the conical head is arranged in the conical through hole, a gap is arranged between the conical head and the conical through hole, and a capillary tube is clamped and fixed through the adjustment gap; extending the capillary tube into the nutrient solution bottle, and sucking the nutrient solution by utilizing siphon action; taking the capillary vertically from the nutrient solution bottle, and recording the liquid level height by using marks when the liquid level of the capillary is stable; vertically inserting the capillary into the conical through hole, fixing the capillary by using a conical head, culturing for a specified time, vertically taking out the capillary, and calculating the food consumption of bees according to the front-back difference of the liquid of the measured capillary;
wherein the nutrient solution is syrup;
Wherein, the air channel is internally provided with a water absorbing material which is adhered with pure water, so that the air in the cup body is kept in a moist state.
2. The measuring device for measuring bee food intake according to claim 1, wherein the water absorbing material is cotton.
3. The measuring device for bee food intake according to claim 1, wherein the length of the capillary tube extending into the cup is perpendicular to the bottom of the cup.
4. The measuring device for bee food intake according to claim 1, wherein a scale is provided on the side wall of the nutrient solution bottle.
5. The measuring device for measuring food intake of bees according to claim 1, wherein the capillary has an inner diameter of 0.3 to 0.5 mm.
6. A measuring method using the measuring device for measuring food intake of bees according to any one of the preceding claims 1 to 5, characterized in that the measuring method comprises the steps of:
s1, measuring the liquid level height of the nutrient solution in a nutrient solution bottle to be a first liquid level value;
s2, after bees are placed in the cup body and the cover body is covered for feeding, the liquid level of the nutrient solution is a second liquid level value;
And S3, obtaining the liquid level height variation according to the difference value between the first liquid level value and the second liquid level value.
7. The method of measuring a bee food intake according to claim 6, wherein step S3 further comprises calculating the total mass of bee food intake based on the level height variation value.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911360326.4A CN111076787B (en) | 2019-12-25 | 2019-12-25 | Measuring device and measuring method for measuring food intake of bees |
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| CN201911360326.4A CN111076787B (en) | 2019-12-25 | 2019-12-25 | Measuring device and measuring method for measuring food intake of bees |
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| CN111076787A CN111076787A (en) | 2020-04-28 |
| CN111076787B true CN111076787B (en) | 2024-06-11 |
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| CN112557256B (en) * | 2020-12-23 | 2024-05-28 | 青岛大学 | Method for testing moisture retention of solution |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN211042388U (en) * | 2019-12-25 | 2020-07-17 | 中国农业科学院蜜蜂研究所 | Measuring device for measuring bee food intake |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1420069A (en) * | 1972-02-15 | 1976-01-07 | Boc International Ltd | Liquid level detector |
| CN2798084Y (en) * | 2004-11-30 | 2006-07-19 | 万华普曼生物工程有限公司 | Sampling detecting cup for liquid sample |
| EP2333509A1 (en) * | 2009-12-08 | 2011-06-15 | Dingens BG bvba | Precision Anaeroid barometer with a capillary tube as a pressure indicator. |
| CN205470562U (en) * | 2016-03-24 | 2016-08-17 | 中国农业科学院蜜蜂研究所 | Honeybee sample receiving flask |
| CN207461874U (en) * | 2017-03-31 | 2018-06-08 | 上海榛果家居用品有限公司 | A kind of water glass |
| CN207355239U (en) * | 2017-09-30 | 2018-05-15 | 福建省农业科学院植物保护研究所 | A kind of easy honeybee indoor toxicity experimental rig |
| CN207569413U (en) * | 2017-12-11 | 2018-07-03 | 张宗炜 | Capillary liquid body feeding |
| CN110313420A (en) * | 2019-07-24 | 2019-10-11 | 贵州省畜牧兽医研究所 | A kind of bees feeder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN211042388U (en) * | 2019-12-25 | 2020-07-17 | 中国农业科学院蜜蜂研究所 | Measuring device for measuring bee food intake |
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