CN111051533B - 消除再生试剂残留的方法 - Google Patents
消除再生试剂残留的方法 Download PDFInfo
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- CN111051533B CN111051533B CN201780094653.2A CN201780094653A CN111051533B CN 111051533 B CN111051533 B CN 111051533B CN 201780094653 A CN201780094653 A CN 201780094653A CN 111051533 B CN111051533 B CN 111051533B
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- 238000006479 redox reaction Methods 0.000 description 1
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- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 229960002317 succinimide Drugs 0.000 description 1
- 125000000446 sulfanediyl group Chemical group *S* 0.000 description 1
- PXQLVRUNWNTZOS-UHFFFAOYSA-N sulfanyl Chemical compound [SH] PXQLVRUNWNTZOS-UHFFFAOYSA-N 0.000 description 1
- 125000004434 sulfur atom Chemical group 0.000 description 1
- XTQHKBHJIVJGKJ-UHFFFAOYSA-N sulfur monoxide Chemical compound S=O XTQHKBHJIVJGKJ-UHFFFAOYSA-N 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 125000004213 tert-butoxy group Chemical group [H]C([H])([H])C(O*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 150000003536 tetrazoles Chemical class 0.000 description 1
- VLLMWSRANPNYQX-UHFFFAOYSA-N thiadiazole Chemical compound C1=CSN=N1.C1=CSN=N1 VLLMWSRANPNYQX-UHFFFAOYSA-N 0.000 description 1
- CBDKQYKMCICBOF-UHFFFAOYSA-N thiazoline Chemical compound C1CN=CS1 CBDKQYKMCICBOF-UHFFFAOYSA-N 0.000 description 1
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- BRNULMACUQOKMR-UHFFFAOYSA-N thiomorpholine Chemical compound C1CSCCN1 BRNULMACUQOKMR-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
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- Biotechnology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (20)
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102140523A (zh) * | 2011-01-28 | 2011-08-03 | 东南大学 | 高通量测序模板的原位复制及其增加阅读长度的测序方法 |
CN102329884A (zh) * | 2011-10-20 | 2012-01-25 | 东南大学 | 两核苷酸同时合成dna测序方法及其应用 |
CN102971335A (zh) * | 2009-03-23 | 2013-03-13 | 史蒂芬·阿尔伯特·本纳 | 可逆终止引物延伸试剂 |
CN103484106A (zh) * | 2013-09-05 | 2014-01-01 | 上海交通大学 | 四色荧光标记可逆终端及其在dna测序中的用途 |
CN106117288A (zh) * | 2015-05-08 | 2016-11-16 | 桑特里莱恩科技控股公司 | 二硫键连接的可逆终止子 |
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US9279149B2 (en) * | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN102971335A (zh) * | 2009-03-23 | 2013-03-13 | 史蒂芬·阿尔伯特·本纳 | 可逆终止引物延伸试剂 |
CN102140523A (zh) * | 2011-01-28 | 2011-08-03 | 东南大学 | 高通量测序模板的原位复制及其增加阅读长度的测序方法 |
CN102329884A (zh) * | 2011-10-20 | 2012-01-25 | 东南大学 | 两核苷酸同时合成dna测序方法及其应用 |
CN103484106A (zh) * | 2013-09-05 | 2014-01-01 | 上海交通大学 | 四色荧光标记可逆终端及其在dna测序中的用途 |
CN106117288A (zh) * | 2015-05-08 | 2016-11-16 | 桑特里莱恩科技控股公司 | 二硫键连接的可逆终止子 |
Non-Patent Citations (1)
Title |
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"A new class of cleavable fluorescent nucleotides: synthesis and optimization as reversible terminators for DNA sequencing by synthesis;Gerardo Turcatti等;《Nucleic Acids Res》;摘要和第3-4页 * |
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