CN111051514A - 基于多聚蛋白对复杂生物系统的重构方法，及其在高活性超简化固氮体系构建中的应用 - Google Patents

基于多聚蛋白对复杂生物系统的重构方法，及其在高活性超简化固氮体系构建中的应用 Download PDF

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CN111051514A
CN111051514A CN201880038612.6A CN201880038612A CN111051514A CN 111051514 A CN111051514 A CN 111051514A CN 201880038612 A CN201880038612 A CN 201880038612A CN 111051514 A CN111051514 A CN 111051514A
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vector
cleav
genes
protease
pseudomonas
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杨建国
谢夏青
相楠
田哲贤
迪克森·瑞
王忆平
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Peking University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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Abstract

本公开提供了表达方法、载体和载体组合物。具体而言，本公开提供了用于在宿主细胞中外源表达复杂生物学系统的方法，以及用于所述方法的载体和载体组合物。

Description

PCT国内申请，说明书已公开。

Claims (116)

  1. PCT国内申请，权利要求书已公开。
CN201880038612.6A 2018-05-11 2018-05-11 基于多聚蛋白对复杂生物系统的重构方法，及其在高活性超简化固氮体系构建中的应用 Pending CN111051514A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/086490 WO2019213939A1 (zh) 2018-05-11 2018-05-11 基于多聚蛋白对复杂生物系统的重构方法，及其在高活性超简化固氮体系构建中的应用

Publications (1)

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CN111051514A true CN111051514A (zh) 2020-04-21

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CN201880038612.6A Pending CN111051514A (zh) 2018-05-11 2018-05-11 基于多聚蛋白对复杂生物系统的重构方法，及其在高活性超简化固氮体系构建中的应用

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US (1) US20220119822A9 (zh)
CN (1) CN111051514A (zh)
WO (1) WO2019213939A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111321185B (zh) * 2018-12-13 2022-04-05 中国科学院天津工业生物技术研究所 一种用于生产高粘度黄原胶的工程菌及其构建方法与应用
CN111925974B (zh) * 2020-05-21 2022-03-11 中国农业科学院生物技术研究所 泌铵与氮高亲模块偶联的人工联合固氮体系

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690808A (zh) * 2011-03-23 2012-09-26 北京大学 为真核表达的目的构建原核基因表达岛
WO2012174271A2 (en) * 2011-06-16 2012-12-20 The Regents Of The University Of California Synthetic gene clusters
WO2015192383A1 (en) * 2014-06-20 2015-12-23 Peking University Iron only nitrogenase system with minimal genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690808A (zh) * 2011-03-23 2012-09-26 北京大学 为真核表达的目的构建原核基因表达岛
WO2012174271A2 (en) * 2011-06-16 2012-12-20 The Regents Of The University Of California Synthetic gene clusters
WO2015192383A1 (en) * 2014-06-20 2015-12-23 Peking University Iron only nitrogenase system with minimal genes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
KARSTEN TEMME 等: "Refactoring the nitrogen fixation gene cluster from Klebsiella oxytoca", 《PNAS》 *
MARY ELIZABETH WAHL: "A synthetic yeast model for differentiation and division of labor", 《A DISSERTATION FOR THE DEGREE OF DOCTOR OF PHILOSOPHY，HARVARD UNIVERSITY》 *
MICHAEL J SMANSKI 等: "Functional optimization of gene clusters by combinatorial design and assembly", 《NATURE BIOTECHNOLOGY》 *
XIA WANG等: "Using Synthetic Biology to Distinguish and Overcome Regulatory and Functional Barriers Related to Nitrogen Fixation", 《PLOS ONE》 *

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US20220119822A9 (en) 2022-04-21
WO2019213939A1 (zh) 2019-11-14

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