CN111019878A - Recombinant escherichia coli with improved L-threonine yield as well as construction method and application thereof - Google Patents
Recombinant escherichia coli with improved L-threonine yield as well as construction method and application thereof Download PDFInfo
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- CN111019878A CN111019878A CN202010032418.6A CN202010032418A CN111019878A CN 111019878 A CN111019878 A CN 111019878A CN 202010032418 A CN202010032418 A CN 202010032418A CN 111019878 A CN111019878 A CN 111019878A
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- escherichia coli
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- threonine
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01003—Homoserine dehydrogenase (1.1.1.3)
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01103—L-Threonine 3-dehydrogenase (1.1.1.103)
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- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
- C12Y103/01026—Dihydrodipicolinate reductase (1.3.1.26)
Abstract
Description
Ingredients | Concentration (g/L) |
Sucrose (fen xiao) | 30 |
|
5 |
|
2 |
|
1 |
Corn steep liquor |
3 |
FeSO4·7H2O | 0.1 |
MnSO4·H2O | 0.1 |
Ingredients | Concentration (g/L) |
Sucrose (Sterilization alone) | 30 |
Potassium dihydrogen phosphate | 1.7 |
Betaine | 1.5 |
Potassium chloride | 0.5 |
|
5 |
|
1 |
Corn steep liquor |
3 |
FeSO4·7H2O | 0.1 |
MnSO4·H2O | 0.1 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010032418.6A CN111019878B (en) | 2020-01-13 | 2020-01-13 | Recombinant escherichia coli with improved L-threonine yield as well as construction method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010032418.6A CN111019878B (en) | 2020-01-13 | 2020-01-13 | Recombinant escherichia coli with improved L-threonine yield as well as construction method and application thereof |
Publications (2)
Publication Number | Publication Date |
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CN111019878A true CN111019878A (en) | 2020-04-17 |
CN111019878B CN111019878B (en) | 2021-04-16 |
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CN (1) | CN111019878B (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111471638A (en) * | 2020-05-22 | 2020-07-31 | 江南大学 | Construction and application of corynebacterium glutamicum mutant strain capable of producing L-homoserine |
CN114317389A (en) * | 2021-12-22 | 2022-04-12 | 南京工业大学 | Method for producing L-threonine by co-culture fermentation of recombinant escherichia coli |
WO2022094847A1 (en) * | 2020-11-05 | 2022-05-12 | 中国科学院深圳先进技术研究院 | Cell lysis solution of engineering bacterium and use thereof in tumor therapy |
CN115011620A (en) * | 2022-05-19 | 2022-09-06 | 江南大学 | Escherichia coli recombinant nucleic acid, recombinant escherichia coli, culture method and method for biologically synthesizing L-threonine |
WO2023142848A1 (en) * | 2022-01-26 | 2023-08-03 | 廊坊梅花生物技术开发有限公司 | Promoter, threonine-producing recombinant microorganism and use thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1597974A (en) * | 2003-09-17 | 2005-03-23 | 广东肇庆星湖生物科技股份有限公司 | Method for producing L-threonine by Escherichia coli |
CN101580813A (en) * | 2008-05-12 | 2009-11-18 | 长春大成实业集团有限公司 | Method for producing L-threonine by fermentation |
CN101939412A (en) * | 2007-09-04 | 2011-01-05 | 味之素株式会社 | Amino acid-producing microorganism and method of producing amino acid |
CN105400801A (en) * | 2015-11-18 | 2016-03-16 | 中国科学院微生物研究所 | ThrA gene mutant for relieving feedback inhibition and application thereof |
CN105543156A (en) * | 2016-03-02 | 2016-05-04 | 廊坊梅花生物技术开发有限公司 | Recombinant strain and preparation method and application thereof |
CN106062176A (en) * | 2014-01-02 | 2016-10-26 | 特雷里斯公司 | Compositions and methods for biological production of amino acids in hydrogenotrophic microorganisms |
-
2020
- 2020-01-13 CN CN202010032418.6A patent/CN111019878B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1597974A (en) * | 2003-09-17 | 2005-03-23 | 广东肇庆星湖生物科技股份有限公司 | Method for producing L-threonine by Escherichia coli |
CN101939412A (en) * | 2007-09-04 | 2011-01-05 | 味之素株式会社 | Amino acid-producing microorganism and method of producing amino acid |
CN101580813A (en) * | 2008-05-12 | 2009-11-18 | 长春大成实业集团有限公司 | Method for producing L-threonine by fermentation |
CN106062176A (en) * | 2014-01-02 | 2016-10-26 | 特雷里斯公司 | Compositions and methods for biological production of amino acids in hydrogenotrophic microorganisms |
CN105400801A (en) * | 2015-11-18 | 2016-03-16 | 中国科学院微生物研究所 | ThrA gene mutant for relieving feedback inhibition and application thereof |
CN105543156A (en) * | 2016-03-02 | 2016-05-04 | 廊坊梅花生物技术开发有限公司 | Recombinant strain and preparation method and application thereof |
Non-Patent Citations (1)
Title |
---|
张力等: "dapA基因缺失对大肠杆菌L-苏氨酸产量的影响", 《甘肃农业大学学报》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111471638A (en) * | 2020-05-22 | 2020-07-31 | 江南大学 | Construction and application of corynebacterium glutamicum mutant strain capable of producing L-homoserine |
CN111471638B (en) * | 2020-05-22 | 2021-11-23 | 江南大学 | Construction and application of corynebacterium glutamicum mutant strain capable of producing L-homoserine |
WO2022094847A1 (en) * | 2020-11-05 | 2022-05-12 | 中国科学院深圳先进技术研究院 | Cell lysis solution of engineering bacterium and use thereof in tumor therapy |
CN114317389A (en) * | 2021-12-22 | 2022-04-12 | 南京工业大学 | Method for producing L-threonine by co-culture fermentation of recombinant escherichia coli |
WO2023142848A1 (en) * | 2022-01-26 | 2023-08-03 | 廊坊梅花生物技术开发有限公司 | Promoter, threonine-producing recombinant microorganism and use thereof |
CN115011620A (en) * | 2022-05-19 | 2022-09-06 | 江南大学 | Escherichia coli recombinant nucleic acid, recombinant escherichia coli, culture method and method for biologically synthesizing L-threonine |
CN115011620B (en) * | 2022-05-19 | 2023-11-07 | 江南大学 | Recombinant nucleic acid of escherichia coli, recombinant escherichia coli, culture method and method for biosynthesis of L-threonine |
Also Published As
Publication number | Publication date |
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CN111019878B (en) | 2021-04-16 |
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Inventor after: Liu Long Inventor after: Zhou Renkai Inventor after: Zhai Xiuchao Inventor after: Chen Taichi Inventor after: Lv Xueqin Inventor after: Li Jianghua Inventor after: Du Guocheng Inventor before: Liu Long Inventor before: Zhou Renkai Inventor before: Zhai Xiuchao Inventor before: Chen Taichi Inventor before: Lv Xueqin Inventor before: Li Jianghua Inventor before: Du Guocheng |
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Effective date of registration: 20210401 Address after: No. 1800, Lihu Avenue, Binhu District, Wuxi City, Jiangsu Province, 214100 Applicant after: Jiangnan University Applicant after: COFCO Engineering (Wuxi) International Biochemical Technology Co.,Ltd. Address before: 1800 No. 214122 Jiangsu city of Wuxi Province Li Lake Avenue Applicant before: Jiangnan University Applicant before: COFCO ENGINEERING & TECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20211228 Address after: No. 1800, Lihu Avenue, Binhu District, Wuxi City, Jiangsu Province, 214100 Patentee after: Jiangnan University Patentee after: Wuxi COFCO Engineering Technology Co., Ltd Address before: No. 1800, Lihu Avenue, Binhu District, Wuxi City, Jiangsu Province, 214100 Patentee before: Jiangnan University Patentee before: COFCO Engineering (Wuxi) International Biochemical Technology Co., Ltd |