CN111013518A - Sound tweezers device and particle control method - Google Patents
Sound tweezers device and particle control method Download PDFInfo
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- CN111013518A CN111013518A CN201911290838.8A CN201911290838A CN111013518A CN 111013518 A CN111013518 A CN 111013518A CN 201911290838 A CN201911290838 A CN 201911290838A CN 111013518 A CN111013518 A CN 111013518A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/08—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
- B01J19/10—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor employing sonic or ultrasonic vibrations
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G—PHYSICS
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- G01S—RADIO DIRECTION-FINDING; RADIO NAVIGATION; DETERMINING DISTANCE OR VELOCITY BY USE OF RADIO WAVES; LOCATING OR PRESENCE-DETECTING BY USE OF THE REFLECTION OR RERADIATION OF RADIO WAVES; ANALOGOUS ARRANGEMENTS USING OTHER WAVES
- G01S15/00—Systems using the reflection or reradiation of acoustic waves, e.g. sonar systems
- G01S15/88—Sonar systems specially adapted for specific applications
- G01S15/89—Sonar systems specially adapted for specific applications for mapping or imaging
- G01S15/8906—Short-range imaging systems; Acoustic microscope systems using pulse-echo techniques
- G01S15/8997—Short-range imaging systems; Acoustic microscope systems using pulse-echo techniques using synthetic aperture techniques
Abstract
The invention provides a sound tweezers device and a method for controlling particles, wherein the method comprises the following steps: a transducer array comprising a plurality of transducers arranged in an array; the control system is connected with the transducer array and used for controlling the transducer array to emit multi-point focused ultrasonic waves in a first period and emit multi-angle plane ultrasonic waves in a second period, the first period and the second period are alternately performed, a stereo ultrasonic image is formed according to the plane ultrasonic waves reflected by the particles received by the transducer array, parameters of the multi-point focused ultrasonic waves emitted by any transducer are adjusted according to the stereo ultrasonic image, the positions of the particles are controlled by changing the positions of a plurality of focuses of the focused ultrasonic waves emitted by the transducer array, and therefore the particle control can be guided and monitored through the stereo ultrasonic image, and the particle control accuracy of the acoustic tweezers device is improved.
Description
Technical Field
The invention relates to the technical field of sound control, in particular to a sound tweezers device and a particle control method thereof.
Background
Similar to optical tweezers, acoustic tweezers can manipulate microparticles by applying acoustic radiation force on the microparticles. Based on the difference between the sound wave and the light wave, the acoustic tweezers have some advantages over the optical tweezers, mainly: 1. the acoustic tweezers have no requirement on the optical transparency of the transmission medium and can be carried out in a non-transparent medium, so that the acoustic tweezers can be applied to various media such as air, water and even a living body theoretically; 2. the trapping force of the acoustic tweezers under unit input energy is far greater than that of the optical tweezers, so that particles with the same size can be trapped by using lower energy, the risk of damaging the particles is reduced, or particles with larger size are trapped under the same energy, such as the manipulation of the particles in centimeter level is realized. These advantages make the acoustic tweezers very suitable for applications in the biomedical field.
When the acoustic tweezers are applied to complex environments such as living bodies, the first problem is that the environments and structures of the living bodies are very complex, and flexible and real-time three-dimensional control of particles is required. Secondly, the medium that propagates in an environment such as a living body is non-transparent, and it is difficult to observe the manipulation of the microparticles with the naked eye or a camera.
At present, acoustic tweezers based on a transducer array have been one of the main methods for particle acoustic manipulation because the sound field manipulated by the acoustic tweezers can dynamically change in real time, can complete complex manipulation behaviors, and the like. However, the manipulation of the particles by the acoustic tweezers cannot be observed and monitored, so that the acoustic manipulation of the particles by the acoustic tweezers is low in accuracy.
Disclosure of Invention
In view of this, the present invention provides an acoustic tweezers device and a method for controlling particles, so as to improve the accuracy of acoustic control of the particles.
In order to achieve the purpose, the invention provides the following technical scheme:
an acoustic tweezers device comprising:
a transducer array comprising a plurality of transducers arranged in an array;
the control system is connected with the transducer array and used for controlling the transducer array to emit multi-point focused ultrasonic waves in a first period and emit multi-angle plane ultrasonic waves in a second period, the first period and the second period are alternately performed, a stereoscopic ultrasonic image is formed according to the plane ultrasonic waves reflected by the particles received by the transducer array, and parameters of the multi-point focused ultrasonic waves emitted by any transducer are adjusted according to the stereoscopic ultrasonic image so as to control the positions of the particles by changing the positions of a plurality of focuses of focused ultrasonic waves emitted by the transducer array.
Optionally, before manipulating the position of the particle, the control system is further configured to obtain a first position of the particle according to an image of a first imaging plane, estimate a second position of the particle on a second imaging plane according to the first position, and move a position of one focus of the focused ultrasound waves emitted by the transducer array to the second position to capture the particle;
wherein the first imaging plane and the second imaging plane are both parallel to the transducer array face and the second imaging plane is located between the first imaging plane and the transducer array.
Optionally, the transducer is 2mm by 2mm to 3mm by 3mm in size; the center distance of the transducers is 2 mm-3 mm.
Optionally, the center frequency of the transducer array is 1MHz to 1.5 MHz.
Optionally, the transducer array is a planar array, a linear array, an annular array, or a curved array.
Optionally, the transducer array is a planar array comprising 16 x 16 transducers, the transducer array having dimensions of 50mm x 50 mm.
Optionally, the control system forms a stereoscopic ultrasound image by using a multi-angle plane wave compounding method, a synthetic aperture imaging method or a divergent-propagation imaging method.
A method for manipulating particles, applied to the acoustic tweezers device as described in any one of the above, comprises:
controlling a transducer array to emit multi-point focused ultrasonic waves in a first time period and multi-angle plane ultrasonic waves in a second time period, wherein the first time period and the second time period are alternately carried out;
forming a stereoscopic ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array;
and adjusting parameters of the multi-point focused ultrasonic waves emitted by any one transducer according to the stereo ultrasonic image so as to control the positions of the particles by changing the positions of a plurality of focuses of the focused ultrasonic waves emitted by the transducer array.
Optionally, before manipulating the position of the particle, the method further comprises:
obtaining a first position of the particle from an image of a first imaging plane;
estimating a second position of the particle on a second imaging plane according to the first position;
moving a position of a focal point of focused ultrasound waves emitted by the transducer array to the second position, capturing the particles;
wherein the first imaging plane and the second imaging plane are both parallel to the transducer array face and the second imaging plane is located between the first imaging plane and the transducer array.
Optionally, forming a stereoscopic ultrasound image from the particle-reflected planar ultrasound waves received by the transducer array comprises:
and forming a three-dimensional ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array by adopting a multi-angle plane wave compounding method, a synthetic aperture imaging method or a divergent broadcasting imaging method.
Compared with the prior art, the technical scheme provided by the invention has the following advantages:
the sound tweezers device and the particle control method provided by the invention divide the time period of ultrasonic wave emission of the transducer array into a first time period and a second time period which are alternately carried out, control the transducer array to emit multi-point focused ultrasonic wave in the first time period and emit multi-angle plane ultrasonic wave in the second time period, form a stereo ultrasonic image according to the plane ultrasonic wave reflected by the particles received by the transducer array, and adjust the parameters of the multi-point focused ultrasonic wave emitted by any transducer according to the stereo ultrasonic image so as to control the position of the particles by changing the position of the focus of the focused ultrasonic wave emitted by the transducer array, so that the particle control can be guided and monitored through the stereo ultrasonic image, and the particle control accuracy of the sound tweezers device is further improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an acoustic tweezers device according to an embodiment of the present invention;
fig. 2 is a distribution diagram of an x-y plane sound field generated by a sound tweezers device according to an embodiment of the present invention;
fig. 3 is a distribution diagram of an x-z plane sound field generated by an acoustic tweezer apparatus according to an embodiment of the present invention;
fig. 4 is a schematic diagram illustrating an experimental result of three PDMS particles captured by a sound field generated by a sound tweezer device according to an embodiment of the present invention;
FIG. 5 is a timing diagram illustrating control of a transducer array according to an embodiment of the present invention;
fig. 6 is a schematic diagram of an acoustic tweezer apparatus for manipulating a single PDMS microparticle according to an embodiment of the present invention;
fig. 7 is a schematic diagram of a sound field generated by the acoustic tweezer apparatus according to the present invention for manipulating a single PDMS particle;
FIG. 8 is a schematic diagram of an ultrasound image of an X-Z plane when a single PDMS particle is manipulated according to an embodiment of the present invention;
FIG. 9 is an ultrasound image of an X-Y plane for manipulating a single PDMS particle according to an embodiment of the present invention;
FIG. 10 is a schematic diagram of an ultrasound image of a Y-Z plane when a single PDMS particle is manipulated according to an embodiment of the present invention;
fig. 11 is a schematic diagram of a PDMS particle captured by an acoustic tweezers device according to an embodiment of the present invention;
fig. 12 is a flowchart of a method for manipulating particles by a sonic tweezer device according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, so that the above is the core idea of the present invention, and the above objects, features and advantages of the present invention can be more clearly understood. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
An embodiment of the present invention provides an acoustic tweezers device, as shown in fig. 1, including a transducer array 1 and a control system (not shown in the figure), where the transducer array 1 includes a plurality of transducers 10 arranged in an array.
The control system is connected with the transducer array 1, specifically, the control system is connected with each transducer 10 in the transducer array 1, the control system is used for controlling the transducer array 1 to emit multi-point focused ultrasonic waves in a first period and multi-angle plane ultrasonic waves in a second period, the first period and the second period are performed alternately, a stereoscopic ultrasonic image is formed according to the plane ultrasonic waves reflected by particles received by the transducer array 1, parameters of the multi-point focused ultrasonic waves emitted by any transducer 10 are adjusted according to the stereoscopic ultrasonic image, and the positions of the particles are controlled by changing the position of a focus of the focused ultrasonic waves emitted by the transducer array 1.
Wherein the control system can adjust the phase, amplitude and waveform of the multi-point focused ultrasound waves emitted by any of the transducers 10 to change the position of the focal point of the focused ultrasound waves emitted by the transducer array 1 by adjusting the phase and/or amplitude of any of the transducers 10.
In the embodiment of the invention, the time interval of transmitting the ultrasonic waves by the transducer array 1 is divided into a first time interval and a second time interval which are alternately performed, the transducer array 1 is controlled to transmit multi-point focused ultrasonic waves in the first time interval and transmit multi-angle plane ultrasonic waves in the second time interval, and a stereo ultrasonic image is formed according to the plane ultrasonic waves reflected by the particles received by the transducer array 1, so that the particles can be guided and monitored through the stereo ultrasonic image, and the particle control accuracy of the acoustic tweezers device is improved.
In the embodiment of the present invention, the transducer array 1 is merely used as a planar array for example, but the present invention is not limited thereto, and in other embodiments, the transducer array 1 may be a linear array, an annular array, an arc array, or the like. As shown in fig. 1, the planar transducer array 1 may comprise 16 x 16, i.e. 256 transducers 10.
Optionally, in the present invention, each transducer 10 has a size of 2mm by 2mm to 3mm by 3mm, preferably 2.6mm by 2.6 mm; the distance between the centers of the transducers 10 is 2mm to 3mm, preferably 2.8mm (about 2 times the wavelength of the emitted ultrasonic waves); the center frequency of the transducer array 1 is 1MHz to 1.5MHz, preferably 1.04 MHz. Based on this, the size of the 16 × 16 planar transducer array 1 is 50mm × 50 mm. Because the transducer array 1 in the embodiment of the present invention has a small size and a high frequency, the acoustic tweezer device in the embodiment of the present invention can manipulate particles in an aqueous environment.
In the embodiment of the present invention, the control system is an improvement on the Verasonics Vantage 256 system, and the control system can independently control the transmission phase, amplitude and waveform of each transducer 10, so as to change the position of the focus of the focused ultrasound transmitted by the transducer array 1 by adjusting the phase and/or amplitude of any transducer 10. The control system may calculate an excitation signal required for the transducer array 1 when a plurality of focuses are realized in a three-dimensional space by using a pseudo-inverse algorithm, and may introduce the excitation signal into the transducer array 1 to form a predetermined multi-focus sound field.
In the embodiment of the present invention, the manipulated particles are PDMS (Polydimethylsiloxane) particles, and the direction of the acoustic radiation force applied to the particles is directed to the strong field position, so that the particles can be captured to the focal position of the multi-focus acoustic field, and three-dimensional manipulation of a plurality of PDMS particles can be realized by changing the multi-focus position in real time. Taking three focuses of different depths as an example, the distances between the three focuses and the surface of the transducer array 1 are respectively 50mm, 60mm and 70mm, the transverse distance between two adjacent focuses is 10mm, the measured transverse, i.e., x-y, plane sound field distribution is shown in fig. 2, the measured axial, i.e., x-z, plane sound field distribution is shown in fig. 3, and the experimental result of capturing three PDMS particles by using the sound field is shown in fig. 4.
In the embodiment of the present invention, as shown in fig. 5, the control system inserts the imaging sequence into the control sequence, that is, the time period in which the transducer array 1 transmits the ultrasonic waves is divided into a first time period and a second time period which are performed alternately, transmits the pulse signal for forming the multi-point focused ultrasonic waves in the control sequence, that is, the first time period, to perform particle control, transmits the pulse signal of the multi-angle planar ultrasonic waves in the imaging sequence, that is, the second time period, and performs imaging by using a multi-angle planar wave composite method according to the echo signal received by the transducer array 1, so as to implement three-dimensional acoustic tweezers under image monitoring.
It should be noted that, the control system in the embodiment of the present invention may form a stereoscopic ultrasound image by using a multi-angle plane wave compounding method, and may also form a stereoscopic ultrasound image by using a synthetic aperture imaging method or a divergent propagation imaging method, which is not described herein any more.
The schematic diagram of the acoustic tweezer device for manipulating a single PDMS particle is shown in fig. 6, the acoustic tweezer experiment for manipulating a single PDMS particle is shown in fig. 7, and the ultrasound images of the X-Z, X-Y and Y-Z planes are shown in fig. 8, 9 and 10, respectively, when manipulating a single PDMS particle.
Since ultrasound imaging does not require the use of optically transparent media, we are assured of seeing the state of motion of the particles as they are manipulated, even in non-transparent media. Meanwhile, the ultrasonic image can be used for guiding the three-dimensional acoustic tweezers. Before the particles are manipulated to reach the designated positions, the control system is further used for obtaining a first position of the particles according to the image of the first imaging plane, estimating a second position of the particles on the second imaging plane according to the first position, and moving the position of one focus of the focused ultrasonic waves emitted by the transducer array 1 to the second position to capture the particles; wherein the first imaging plane and the second imaging plane are both parallel to the surface of the transducer array 1, and the second imaging plane is located between the first imaging plane and the transducer array 1.
As shown in fig. 11, two imaging planes, i.e., X-Y imaging planes (Z ═ Z), are arranged above the transducer array 1 in a direction parallel to the surface of the transducer array 10) And a second, X-Y, imaging plane (Z ═ Z)1). When the next PDMS particle is thrown in any position in the imaging volume, it falls freely, as it falls through the X-Y imaging plane (Z ═ Z)0) The control system may pass through an X-Y imaging plane (Z ═ Z)0) The generated ultrasonic image locks the transverse position of the PDMS particle falling, namely the first position (X) of the PDMS particle at the moment is obtained0，Y0，Z0). The control system can then estimate the position of the PDMS particle in the X-Y imaging plane (Z-Z) based on the first position of the PDMS particle at that time1) Second position (X) on0，Y0，Z1) Immediately thereafter, the focus of the focused ultrasound was moved to the first position (X) at the moment of the PDMS particles0，Y0，Z0) Second right belowPosition (X)0，Y0，Z1)。
When the dropping PDMS particles drop to Z1At height, it will be captured by the focused ultrasound emitted by the control system, thus stopping the fall. After the PDMS particles are captured, the control system can control the PDMS particles to move to any specified position (X) in three-dimensional space by changing the position of the focus1，Y1，Z2) During the movement of the PDMS particles, monitoring by ultrasound images may continue. In the embodiment of the invention, the ultrasonic image guiding sound forceps device is used for guiding and monitoring the particles, so that the problem that the sound forceps start to capture the particles from any initial position is solved, and the usability of the sound forceps is greatly improved.
An embodiment of the present invention further provides a method for manipulating microparticles, which is applied to the acoustic tweezer device provided in any one of the above embodiments, as shown in fig. 12, and the method includes:
s101: controlling the transducer array to emit multi-point focused ultrasonic waves in a first time period and multi-angle plane ultrasonic waves in a second time period, wherein the first time period and the second time period are alternately carried out;
s102: forming a stereoscopic ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array;
s103: parameters of the multi-point focused ultrasound waves emitted by any transducer are adjusted according to the stereo ultrasound image to manipulate the position of the particles by changing the positions of multiple focal points of the focused ultrasound waves emitted by the transducer array.
In the embodiment of the invention, the time period of transmitting ultrasonic waves by the transducer array 1 is divided into a first time period and a second time period which are alternately performed, the transducer array 1 is controlled to transmit multi-point focused ultrasonic waves in the first time period and transmit multi-angle plane ultrasonic waves in the second time period, a three-dimensional ultrasonic image is formed according to the plane ultrasonic waves reflected by particles received by the transducer array 1, the parameters of the multi-point focused ultrasonic waves transmitted by any transducer are adjusted according to the three-dimensional ultrasonic image, so that the positions of the particles are controlled by changing the positions of a plurality of focuses of the focused ultrasonic waves transmitted by the transducer array, the control of the particles can be guided and monitored through the three-dimensional ultrasonic image, and the control accuracy of the acoustic tweezers device on the particles is.
In an embodiment of the present invention, forming a stereoscopic ultrasound image according to planar ultrasonic waves reflected by particles received by a transducer array includes:
and forming a three-dimensional ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array by adopting a multi-angle plane wave compounding method, a synthetic aperture imaging method or a divergent broadcasting imaging method.
Optionally, before manipulating the position of the particle, the method further comprises:
obtaining a first position of the particle from an image of the first imaging plane;
estimating a second position of the particle on a second imaging plane according to the first position;
moving a position of one focus of focused ultrasound waves emitted by the transducer array to a second position to capture particles;
wherein the first imaging plane and the second imaging plane are both parallel to the transducer array face and the second imaging plane is located between the first imaging plane and the transducer array.
As shown in fig. 11, two imaging planes, i.e., X-Y imaging planes (Z ═ Z), are arranged above the transducer array 1 in a direction parallel to the surface of the transducer array 10) And a second, X-Y, imaging plane (Z ═ Z)1). When the next PDMS particle is thrown in any position in the imaging volume, it falls freely, as it falls through the X-Y imaging plane (Z ═ Z)0) The control system may pass through an X-Y imaging plane (Z ═ Z)0) The generated ultrasonic image locks the transverse position of the PDMS particle falling, namely the first position (X) of the PDMS particle at the moment is obtained0，Y0，Z0). The control system can then estimate the position of the PDMS particle in the X-Y imaging plane (Z-Z) based on the first position of the PDMS particle at that time1) Second position (X) on0，Y0，Z1) Immediately thereafter, the focus of the focused ultrasound was moved to the first position (X) at the moment of the PDMS particles0，Y0，Z0) Second position (X) right below0，Y0，Z1). When the dropping PDMS particles drop to Z1At height, it will be captured by the focused ultrasound emitted by the control system, thus stopping the fall. After the PDMS particles are captured, the control system can control the PDMS particles to move to any specified position (X) in three-dimensional space by changing the position of the focus1，Y1，Z2) During the movement of the PDMS particles, monitoring by ultrasound images may continue.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. An acoustic tweezers device, comprising:
a transducer array comprising a plurality of transducers arranged in an array;
the control system is connected with the transducer array and used for controlling the transducer array to emit multi-point focused ultrasonic waves in a first period and emit multi-angle plane ultrasonic waves in a second period, the first period and the second period are alternately performed, a stereoscopic ultrasonic image is formed according to the plane ultrasonic waves reflected by the particles received by the transducer array, and parameters of the multi-point focused ultrasonic waves emitted by any transducer are adjusted according to the stereoscopic ultrasonic image so as to control the positions of the particles by changing the positions of a plurality of focuses of focused ultrasonic waves emitted by the transducer array.
2. The acoustic tweezers device of claim 1, wherein prior to manipulating the position of the particle, the control system is further configured to obtain a first position of the particle based on the image of the first imaging plane, estimate a second position of the particle on the second imaging plane based on the first position, and move a position of a focal point of the focused ultrasound waves emitted by the transducer array to the second position to capture the particle;
wherein the first imaging plane and the second imaging plane are both parallel to the transducer array face and the second imaging plane is located between the first imaging plane and the transducer array.
3. The acoustic tweezers device of claim 1 or 2, wherein the transducers are 2mm by 2mm to 3mm by 3mm in size; the center distance of the transducers is 2 mm-3 mm.
4. The acoustic tweezers device of claim 3, wherein the transducer array has a center frequency of 1MHz to 1.5 MHz.
5. The acoustic tweezers device of claim 1, wherein the transducer array is a planar array, a linear array, an annular array, or a curved array.
6. The acoustic tweezers device of claim 5, wherein the transducer array is a planar array comprising 16 x 16 transducers, the transducer array having dimensions of 50mm x 50 mm.
7. The acoustic tweezers device of claim 1, wherein the control system forms a stereoscopic ultrasound image using a multi-angle plane wave compounding method, a synthetic aperture imaging method, or a divergent-propagation imaging method.
8. A method for manipulating microparticles, applied to the acoustic tweezers device according to any one of claims 1 to 7, comprising:
controlling a transducer array to emit multi-point focused ultrasonic waves in a first time period and multi-angle plane ultrasonic waves in a second time period, wherein the first time period and the second time period are alternately carried out;
forming a stereoscopic ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array;
and adjusting parameters of the multi-point focused ultrasonic waves emitted by any one transducer according to the stereo ultrasonic image so as to control the positions of the particles by changing the positions of a plurality of focuses of the focused ultrasonic waves emitted by the transducer array.
9. The method of claim 8, wherein prior to manipulating the position of the particle, further comprising:
obtaining a first position of the particle from an image of a first imaging plane;
estimating a second position of the particle on a second imaging plane according to the first position;
moving a position of a focal point of focused ultrasound waves emitted by the transducer array to the second position, capturing the particles;
wherein the first imaging plane and the second imaging plane are both parallel to the transducer array face and the second imaging plane is located between the first imaging plane and the transducer array.
10. The method of claim 8, wherein forming a stereoscopic ultrasound image from the particle-reflected planar ultrasound waves received by the transducer array comprises:
and forming a three-dimensional ultrasonic image according to the plane ultrasonic waves reflected by the particles received by the transducer array by adopting a multi-angle plane wave compounding method, a synthetic aperture imaging method or a divergent broadcasting imaging method.
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| CN201911290838.8A CN111013518B (en) | 2019-12-12 | 2019-12-12 | Sound tweezers device and particle control method |
| PCT/CN2020/129485 WO2021115046A1 (en) | 2019-12-12 | 2020-11-17 | Acoustic tweezer device and method for manipulating particles |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112349446A (en) * | 2020-11-03 | 2021-02-09 | 深圳先进技术研究院 | Control method and acoustic tweezers device |
| CN112562631A (en) * | 2020-11-17 | 2021-03-26 | 深圳先进技术研究院 | Method and system for generating acoustic tweezers |
| WO2021115046A1 (en) * | 2019-12-12 | 2021-06-17 | 深圳先进技术研究院 | Acoustic tweezer device and method for manipulating particles |
| CN114146890A (en) * | 2021-12-03 | 2022-03-08 | 深圳先进技术研究院 | Ultrasonic sound control method and sound tweezers device |
| CN114160070A (en) * | 2021-12-02 | 2022-03-11 | 深圳先进技术研究院 | Control method, acoustic tweezers device and microscopic equipment applying acoustic tweezers device |
| CN114426964A (en) * | 2021-12-03 | 2022-05-03 | 深圳先进技术研究院 | Ultrasonic sound control method |
| WO2023102774A1 (en) * | 2021-12-08 | 2023-06-15 | 深圳先进技术研究院 | Acoustic control method and system based on human-machine interaction |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104870077A (en) * | 2012-01-31 | 2015-08-26 | 宾夕法尼亚州立大学研究基金会 | Microfluidic manipulation and sorting of particles using tunable standing surface acoustic wave |
| CN106076450A (en) * | 2016-06-03 | 2016-11-09 | 杭州电子科技大学 | A kind of particle suspension dynamic focusing heating system and heating means thereof |
| CN106154251A (en) * | 2016-06-27 | 2016-11-23 | 中国科学院苏州生物医学工程技术研究所 | Ultrasonic beam synthetic method, ultrasonic imaging method and ultrasonic elastograph imaging method |
| CN106456118A (en) * | 2013-11-19 | 2017-02-22 | 港大科桥有限公司 | Apparatus for ultrasound flow vector imaging and methods thereof |
| CN108025485A (en) * | 2015-10-21 | 2018-05-11 | 神经技术Uab公司 | Contactless commanding apparatus, assemble method and 3D printing |
| US10106397B1 (en) * | 2012-04-23 | 2018-10-23 | University Of Southern California | Acoustic tweezers |
| CN109126918A (en) * | 2018-10-18 | 2019-01-04 | 天津大学 | It is a kind of for generating the device of acoustic streaming body tweezer |
| CN110050218A (en) * | 2016-03-15 | 2019-07-23 | 法国国家科研中心 | Acoustic tweezers |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8316714B2 (en) * | 2009-07-22 | 2012-11-27 | Siemens Medical Solutions Usa, Inc. | Scan patterns for electronically positioned apertures on an array |
| KR102341153B1 (en) * | 2015-05-13 | 2021-12-17 | 가부시키가이샤 에아렉크스 | How to control particles |
| CN105214742B (en) * | 2015-10-10 | 2017-10-31 | 中国科学院深圳先进技术研究院 | The method of microfluid system and manipulation particulate based on artificial structure's sound field |
| CN105233429A (en) * | 2015-11-10 | 2016-01-13 | 中国科学院深圳先进技术研究院 | Method and system for carrying out automatic tracking and positioning as well as real-time intelligent stimulation on target object on the basis of ultrasound imaging |
| CN107050630B (en) * | 2017-04-01 | 2020-12-08 | 深圳先进技术研究院 | System and method for controlling particles based on time reversal technology |
| CN111013518B (en) * | 2019-12-12 | 2020-12-08 | 深圳先进技术研究院 | Sound tweezers device and particle control method |
-
2019
- 2019-12-12 CN CN201911290838.8A patent/CN111013518B/en active Active
-
2020
- 2020-11-17 WO PCT/CN2020/129485 patent/WO2021115046A1/en active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104870077A (en) * | 2012-01-31 | 2015-08-26 | 宾夕法尼亚州立大学研究基金会 | Microfluidic manipulation and sorting of particles using tunable standing surface acoustic wave |
| US10106397B1 (en) * | 2012-04-23 | 2018-10-23 | University Of Southern California | Acoustic tweezers |
| CN106456118A (en) * | 2013-11-19 | 2017-02-22 | 港大科桥有限公司 | Apparatus for ultrasound flow vector imaging and methods thereof |
| CN108025485A (en) * | 2015-10-21 | 2018-05-11 | 神经技术Uab公司 | Contactless commanding apparatus, assemble method and 3D printing |
| CN110050218A (en) * | 2016-03-15 | 2019-07-23 | 法国国家科研中心 | Acoustic tweezers |
| CN106076450A (en) * | 2016-06-03 | 2016-11-09 | 杭州电子科技大学 | A kind of particle suspension dynamic focusing heating system and heating means thereof |
| CN106154251A (en) * | 2016-06-27 | 2016-11-23 | 中国科学院苏州生物医学工程技术研究所 | Ultrasonic beam synthetic method, ultrasonic imaging method and ultrasonic elastograph imaging method |
| CN109126918A (en) * | 2018-10-18 | 2019-01-04 | 天津大学 | It is a kind of for generating the device of acoustic streaming body tweezer |
Non-Patent Citations (1)
| Title |
|---|
| 杨晔等: ""超声图像引导和监控下的三维声镊"", 《声学技术》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021115046A1 (en) * | 2019-12-12 | 2021-06-17 | 深圳先进技术研究院 | Acoustic tweezer device and method for manipulating particles |
| CN112349446A (en) * | 2020-11-03 | 2021-02-09 | 深圳先进技术研究院 | Control method and acoustic tweezers device |
| WO2022095155A1 (en) * | 2020-11-03 | 2022-05-12 | 深圳先进技术研究院 | Control method and acoustic tweezer apparatus |
| CN112562631A (en) * | 2020-11-17 | 2021-03-26 | 深圳先进技术研究院 | Method and system for generating acoustic tweezers |
| CN112562631B (en) * | 2020-11-17 | 2022-04-26 | 深圳先进技术研究院 | Method and system for generating acoustic tweezers |
| CN114160070A (en) * | 2021-12-02 | 2022-03-11 | 深圳先进技术研究院 | Control method, acoustic tweezers device and microscopic equipment applying acoustic tweezers device |
| CN114146890A (en) * | 2021-12-03 | 2022-03-08 | 深圳先进技术研究院 | Ultrasonic sound control method and sound tweezers device |
| CN114426964A (en) * | 2021-12-03 | 2022-05-03 | 深圳先进技术研究院 | Ultrasonic sound control method |
| CN114146890B (en) * | 2021-12-03 | 2022-09-13 | 深圳先进技术研究院 | Ultrasonic sound control method and sound tweezers device |
| WO2023102774A1 (en) * | 2021-12-08 | 2023-06-15 | 深圳先进技术研究院 | Acoustic control method and system based on human-machine interaction |
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| WO2021115046A1 (en) | 2021-06-17 |
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