CN111012802A - Preparation method and identification method of egg yolk oil - Google Patents
Preparation method and identification method of egg yolk oil Download PDFInfo
- Publication number
- CN111012802A CN111012802A CN201911226599.XA CN201911226599A CN111012802A CN 111012802 A CN111012802 A CN 111012802A CN 201911226599 A CN201911226599 A CN 201911226599A CN 111012802 A CN111012802 A CN 111012802A
- Authority
- CN
- China
- Prior art keywords
- solution
- egg yolk
- yolk oil
- steps
- petroleum ether
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Plasma & Fusion (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention relates to a preparation method and an identification method of egg yolk oil, which comprises the following steps: and (3) putting the prepared egg yolk into a pot, heating, and decocting to obtain egg yolk oil. Further comprising the steps of: taking egg yolk oil, adding petroleum ether, washing the solution with an ethanol solution, taking a petroleum ether solution, evaporating the petroleum ether solution to dryness, pouring the evaporated petroleum ether solution into a trichloromethane solution, adding an alumina column into the solution, eluting the solution with petroleum ether and benzene in sequence, eluting with a benzene-acetone mixed solution, collecting an eluent to dryness, pouring residues into 3ml of the trichloromethane solution, sucking a test solution, spotting the test solution on a silica gel G thin-layer plate, developing with benzene-trichloromethane-acetone, taking out and drying, spraying a perchloric acid solution on the dried silica gel G thin-layer plate, and drying to detect an acid value, a saponification value and an iodine value after comparison by a detector.
Description
Technical Field
The invention relates to the field of food processing equipment, in particular to a preparation method and an identification method of egg yolk oil.
Background
The egg yolk oil is oil extracted from egg yolk of eggs, is also called egg fish, phoenix oil and the like, and is a good medicine for treating mild scalds because the egg yolk oil contains rich vitamin A, D, lecithin and the like, has important effects on skin regeneration and metabolism, and has good effect on treating scalds.
In the prior art, the egg yolk oil has rich medicinal value, but the prior preparation method of the egg yolk oil is relatively backward, the extraction rate is relatively low, and the egg yolk oil cannot be identified.
Disclosure of Invention
The invention aims to provide a preparation method and an identification method of egg yolk oil, which are used for solving the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of egg yolk oil comprises the following steps:
1) boiling fresh egg, removing egg shell, and taking out egg yolk for use;
2) putting the egg yolk prepared in the step 1) into a pot, and heating at 80-90 ℃ for 20-30 min;
3) heating to 90-100 deg.C for 20-30min, and decocting to obtain egg yolk oil.
Preferably, the heating in the pot in the step 2) is carried out by mild fire.
More preferably, when the temperature is increased in the step 3), the slow fire is changed into the big fire.
The identification method of egg yolk oil comprises the following steps:
1) taking 2g of egg yolk oil, adding into an open container, adding 50ml of petroleum ether, and stirring in one direction until the egg yolk oil is dissolved;
2) washing the solution with 15ml ethanol solution;
3) repeating the step 2) twice;
4) discarding the ethanol solution, and separating petroleum ether solution;
5) evaporating the petroleum ether solution obtained in the step 4) to dryness to obtain residue;
6) pouring the residue obtained in the step 5) into 2ml of trichloromethane solution to dissolve the residue;
7) adding an alumina column (150 meshes and 200 meshes, the weight of 10g and the inner diameter of 10-15mm) into the solution obtained in the step 6);
8) eluting the solution in the step 7) by using 20ml of petroleum ether and 20ml of benzene in sequence, and discarding the eluent after the elution;
9) then 20ml of benzene-acetone (4: 1) eluting the mixed solution, collecting the eluent, and evaporating to dryness to obtain residue;
10) pouring the residue into 3ml of chloroform solution, and dissolving the residue to obtain a test solution;
11) pipette 2 μ l of the sample solution from step 10) and spot on a silica gel G thin layer plate, and mix the sample solution with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
12) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min, and displaying colored spots for detection personnel to compare and then detect the acid value, the saponification value and the iodine value.
Preferably, the method further comprises the following steps:
13) taking β -sitosterol solution as a reference substance, and adding chloroform to prepare a solution with the density of 1 mg/ml;
14) mu.l of the solution from step 13) was taken and spotted on a silica gel G thin layer plate at the same position as the sample solution, and the surface of the silica gel G thin layer plate was coated with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
15) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min to display colored spots as a reference for detecting the test solution so as to assist a detector in detecting.
The identification method of egg yolk oil comprises the following steps:
1) 1g of egg oil was added with 30ml of glacial acetic acid-chloroform (6: 1) shaking to dissolve egg oil;
2) adding 1ml of potassium iodide saturated solution into the step 1), and shaking for 1 min;
3) adding 100ml of water and 1ml of starch indicator into the solution in the step 2);
4) continuously carrying out twisting titration on the solution in the step 3) by using sodium thiosulfate titration solution until the blue color disappears, and correcting the titration result by using a blank for a detection person to detect the peroxidation value of the egg yolk oil.
7. The method of claim 6, wherein the identification of egg yolk oil comprises: the consumed sodium thiosulfate titration solution in the step 4) is less than or equal to 1.5 ml.
The identification method of egg yolk oil comprises the following steps:
and (4) carrying out microbial limit inspection according to non-sterile products by using a microbial counting method, a bacteria control detection method and a non-sterile medicine microbial limit standard.
Compared with the prior art, the invention has the beneficial effects that: the egg yolk oil prepared by the preparation method has great medicinal value and higher extraction rate, and three identification methods are provided for comprehensively detecting the acid value, the saponification value, the iodine value and the peroxide value of the egg yolk oil, so that the identification effect is achieved, and the activity of the egg yolk oil is more accurately shown; the egg yolk oil prepared by the preparation method can relieve swelling and remove toxicity, heal sores and promote granulation, and is mainly used for eczema, skin itch, scalds, hand and foot rhagadia and the like.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
The invention provides a technical scheme that: a preparation method of egg yolk oil comprises the following steps:
1) boiling fresh egg, removing egg shell, and taking out egg yolk for use;
2) putting the egg yolk prepared in the step 1) into a pot, and heating at 80-90 ℃ for 20-30 min;
3) heating to 90-100 deg.C for 20-30min, and decocting to obtain egg yolk oil.
As an improvement of the scheme, the step 2) is to heat the pot with slow fire.
As an improvement of the scheme, when the temperature is increased in the step 3), the slow fire is changed into the big fire.
Comparative example 1: the king xx, male, 46 years old, suffered from eczema for 3 years, the egg oil is externally applied for one month, the eczema is obviously improved, the egg oil is continuously used for one year and is completely recovered, and the disease is revisited after three years without relapse.
Comparative example 2: horse xx, female, 52 years old, suffering from tinea manus and pedis for 4 years, the egg oil is externally applied to the affected part, the condition is obviously improved, the egg oil is completely recovered after being continuously used for half a year, and the disease is revisited after three years without relapse.
Comparative example 3: the situation is obviously improved when the egg oil is externally applied to the affected part, the affected part is completely recovered after being continuously used for half a year, and the affected part is visited after three years without relapse.
Example two
The identification method of egg yolk oil comprises the following steps:
1) taking 2g of egg yolk oil, adding into an open container, adding 50ml of petroleum ether, and stirring in one direction until the egg yolk oil is dissolved;
2) washing the solution with 15ml ethanol solution;
3) repeating the step 2) twice;
4) discarding the ethanol solution, and separating petroleum ether solution;
5) evaporating the petroleum ether solution obtained in the step 4) to dryness to obtain residue;
6) pouring the residue obtained in the step 5) into 2ml of trichloromethane solution to dissolve the residue;
7) adding an alumina column (150 meshes and 200 meshes, the weight of 10g and the inner diameter of 10-15mm) into the solution obtained in the step 6);
8) eluting the solution in the step 7) by using 20ml of petroleum ether and 20ml of benzene in sequence, and discarding the eluent after the elution;
9) then 20ml of benzene-acetone (4: 1) eluting the mixed solution, collecting the eluent, and evaporating to dryness to obtain residue;
10) pouring the residue into 3ml of chloroform solution, and dissolving the residue to obtain a test solution;
11) pipette 2 μ l of the sample solution from step 10) and spot on a silica gel G thin layer plate, and mix the sample solution with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
12) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min, and displaying colored spots for detection personnel to compare and then detect the acid value, the saponification value and the iodine value.
As a preferable embodiment of the above aspect, the method further includes the steps of:
13) taking β -sitosterol solution as a reference substance, and adding chloroform to prepare a solution with the density of 1 mg/ml;
14) mu.l of the solution from step 13) was taken and spotted on a silica gel G thin layer plate at the same position as the sample solution, and the surface of the silica gel G thin layer plate was coated with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
15) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min to display colored spots as a reference for detecting the test solution so as to assist a detector in detecting.
EXAMPLE III
The identification method of egg yolk oil comprises the following steps:
1) 1g of egg oil was added with 30ml of glacial acetic acid-chloroform (6: 1) shaking to dissolve egg oil;
2) adding 1ml of potassium iodide saturated solution into the step 1), and shaking for 1 min;
3) adding 100ml of water and 1ml of starch indicator into the solution in the step 2);
4) continuously carrying out twisting titration on the solution in the step 3) by using sodium thiosulfate titration solution until the blue color disappears, and correcting the titration result by using a blank for a detection person to detect the peroxidation value of the egg yolk oil.
As a modification of the above, the consumed sodium thiosulfate titration solution in the step 4) is less than or equal to 1.5 ml.
Example four
The identification method of egg yolk oil comprises the following steps:
and (4) carrying out microbial limit inspection according to non-sterile products by using a microbial counting method, a bacteria control detection method and a non-sterile medicine microbial limit standard.
While there have been shown and described what are at present considered the fundamental principles and essential features of the invention and its advantages, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A preparation method of egg yolk oil is characterized by comprising the following steps: the method comprises the following steps:
1) boiling fresh egg, removing egg shell, and taking out egg yolk for use;
2) putting the egg yolk prepared in the step 1) into a pot, and heating at 80-90 ℃ for 20-30 min;
3) heating to 90-100 deg.C for 20-30min, and decocting to obtain egg yolk oil.
2. The method for preparing egg yolk oil according to claim 1, wherein the method comprises the following steps: in the step 2), the pan is heated by slow fire.
3. The method for preparing egg yolk oil according to claim 1 or 2, wherein: and 3) when the temperature is raised in the step 3), the slow fire is changed into the big fire.
4. The identification method of egg yolk oil is characterized by comprising the following steps: the method comprises the following steps:
1) taking 2g of egg yolk oil, adding into an open container, adding 50ml of petroleum ether, and stirring in one direction until the egg yolk oil is dissolved;
2) washing the solution with 15ml ethanol solution;
3) repeating the step 2) twice;
4) discarding the ethanol solution, and separating petroleum ether solution;
5) evaporating the petroleum ether solution obtained in the step 4) to dryness to obtain residue;
6) pouring the residue obtained in the step 5) into 2ml of trichloromethane solution to dissolve the residue;
7) adding an alumina column (150 meshes and 200 meshes, the weight of 10g and the inner diameter of 10-15mm) into the solution obtained in the step 6);
8) eluting the solution in the step 7) by using 20ml of petroleum ether and 20ml of benzene in sequence, and discarding the eluent after the elution;
9) then 20ml of benzene-acetone (4: 1) eluting the mixed solution, collecting the eluent, and evaporating to dryness to obtain residue;
10) pouring the residue into 3ml of chloroform solution, and dissolving the residue to obtain a test solution;
11) pipette 2 μ l of the sample solution from step 10) and spot on a silica gel G thin layer plate, and mix the sample solution with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
12) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min, and displaying colored spots for detection personnel to compare and then detect the acid value, the saponification value and the iodine value.
5. The method for identifying egg yolk oil according to claim 4, wherein: further comprising the steps of:
13) taking β -sitosterol solution as a reference substance, and adding chloroform to prepare a solution with the density of 1 mg/ml;
14) mu.l of the solution from step 13) was taken and spotted on a silica gel G thin layer plate at the same position as the sample solution, and the surface of the silica gel G thin layer plate was coated with benzene-chloroform-acetone (8: 5: 1) after the developing agent is developed, taking out and airing;
15) spraying a 20% perchloric acid solution on the silica gel G thin-layer plate dried in the step 11), drying at 105 ℃ for 5min to display colored spots as a reference for detecting the test solution so as to assist a detector in detecting.
6. The identification method of egg yolk oil is characterized by comprising the following steps: the method comprises the following steps:
1) 1g of egg oil was added with 30ml of glacial acetic acid-chloroform (6: 1) shaking to dissolve egg oil;
2) adding 1ml of potassium iodide saturated solution into the step 1), and shaking for 1 min;
3) adding 100ml of water and 1ml of starch indicator into the solution in the step 2);
4) continuously carrying out twisting titration on the solution in the step 3) by using sodium thiosulfate titration solution until the blue color disappears, and correcting the titration result by using a blank for a detection person to detect the peroxidation value of the egg yolk oil.
7. The method of claim 6, wherein the identification of egg yolk oil comprises: the consumed sodium thiosulfate titration solution in the step 4) is less than or equal to 1.5 ml.
8. The identification method of egg yolk oil is characterized by comprising the following steps: the method comprises the following steps:
and (4) carrying out microbial limit inspection according to non-sterile products by using a microbial counting method, a bacteria control detection method and a non-sterile medicine microbial limit standard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911226599.XA CN111012802A (en) | 2019-12-04 | 2019-12-04 | Preparation method and identification method of egg yolk oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911226599.XA CN111012802A (en) | 2019-12-04 | 2019-12-04 | Preparation method and identification method of egg yolk oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111012802A true CN111012802A (en) | 2020-04-17 |
Family
ID=70207335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911226599.XA Pending CN111012802A (en) | 2019-12-04 | 2019-12-04 | Preparation method and identification method of egg yolk oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111012802A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113786370A (en) * | 2021-11-10 | 2021-12-14 | 广州华夏职业学院 | Whitening and moisturizing mask and preparation equipment thereof |
-
2019
- 2019-12-04 CN CN201911226599.XA patent/CN111012802A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113786370A (en) * | 2021-11-10 | 2021-12-14 | 广州华夏职业学院 | Whitening and moisturizing mask and preparation equipment thereof |
| CN113786370B (en) * | 2021-11-10 | 2023-09-05 | 广州华夏职业学院 | Whitening and moisturizing facial mask mixing equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bergman et al. | Safety, tolerability, pharmacokinetics, and pharmacodynamics of a liver‐targeting acetyl‐CoA carboxylase inhibitor (PF‐05221304): a three‐part randomized phase 1 study | |
| Oboh et al. | Rutin restores neurobehavioral deficits via alterations in cadmium bioavailability in the brain of rats exposed to cadmium | |
| Ozyel et al. | Anti‐inflammatory effects of quercetin on high‐glucose and pro‐inflammatory cytokine challenged vascular endothelial cell metabolism | |
| Matsuoka et al. | Competitive solubilization of cholesterol and six species of sterol/stanol in bile salt micelles | |
| Tang et al. | A new TLC bioautographic assay for qualitative and quantitative estimation of lipase inhibitors | |
| Kushwaha et al. | Antidiabetic effects of isolated sterols from Ficus racemosa leaves | |
| CN111012802A (en) | Preparation method and identification method of egg yolk oil | |
| CN102980969A (en) | Method for screening lipase inhibitor | |
| Zhou et al. | Overview of aristolochic acid nephropathy: an update | |
| Kandrac et al. | Isolation and determination of bile acids | |
| Idler et al. | Steroid hormones in the plasma of spawned Atlantic salmon (Salmo salar) and a comparison of their determination by chemical and biological assay methods | |
| Ibrahim et al. | Biologically-guided isolation of natural lead antithyroid drug from Medicago sativa L. sprouts and its toxic profile in comparison with propylthiouracil | |
| CN101361869B (en) | Quality control method of tables for treating asthma | |
| US3993756A (en) | Antilipemic agent containing a soybean oil fraction | |
| Ma et al. | Simultaneous determination of estrogens and progestogens in honey using high performance liquid chromatography–tandem mass spectrometry | |
| Wybenga et al. | Quantitative determination of 3-methoxy-4-hydroxy-mandelic acid (VMA) in urine | |
| CN104792920B (en) | The inverse thin layer chromatography discrimination method of bile acids composition in a kind of poultry class gallbladder powder | |
| áEl Darawy | The determination of alkaloids in biological material by compound formation with indicators | |
| Steinetz et al. | Influence of Vehicle of Administration on Intestinal Absorption, Fat Storage, and Biological Activity of Ethynylestradiol (EE) and its 3-Cyclopentyl Ethejr (EEGPE) in Rats | |
| Karawya et al. | Colorimetric assay of quinine and quinidine in raw materials, formulations, and biological fluids | |
| Ponder | Fluorometric determination and thin layer chromatographic identification of diethylstilbestrol in beef liver | |
| CN114965809B (en) | Identification method of effective components of cortex phellodendri capable of being salted easily by Huang Shangzhong | |
| Smielova et al. | Selection of the optimal conditions for analysis of plant active pharmaceutical inulin ingredients by thin-layer chromatography to develop the draft for the national monograph “Inulin” | |
| WO2013044568A1 (en) | Method for testing drug for treating coronary artery disease and angina pectoris | |
| Wang et al. | Vine tea total flavonoids activate the AMPK/mTOR pathway to amelioration hepatic steatosis in mice fed a high‐fat diet |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200417 |