CN111011873A - Modification method of rice bran dietary fiber - Google Patents
Modification method of rice bran dietary fiber Download PDFInfo
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- CN111011873A CN111011873A CN201911387833.7A CN201911387833A CN111011873A CN 111011873 A CN111011873 A CN 111011873A CN 201911387833 A CN201911387833 A CN 201911387833A CN 111011873 A CN111011873 A CN 111011873A
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 78
- 235000009566 rice Nutrition 0.000 title claims abstract description 78
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 47
- 238000002715 modification method Methods 0.000 title claims abstract description 14
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 77
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 22
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 22
- 238000005406 washing Methods 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000000926 separation method Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 11
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- 230000007062 hydrolysis Effects 0.000 claims abstract description 6
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- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 22
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 22
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 20
- 239000000706 filtrate Substances 0.000 claims description 16
- 238000000605 extraction Methods 0.000 claims description 13
- 239000002244 precipitate Substances 0.000 claims description 12
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 claims description 11
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 11
- 150000002972 pentoses Chemical class 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 9
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 108091005658 Basic proteases Proteins 0.000 claims description 5
- 238000007605 air drying Methods 0.000 claims description 5
- 102000004139 alpha-Amylases Human genes 0.000 claims description 5
- 108090000637 alpha-Amylases Proteins 0.000 claims description 5
- 229940024171 alpha-amylase Drugs 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 230000035484 reaction time Effects 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 5
- 239000008158 vegetable oil Substances 0.000 claims description 5
- 238000000967 suction filtration Methods 0.000 claims description 2
- 238000007602 hot air drying Methods 0.000 claims 1
- 238000001291 vacuum drying Methods 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 9
- 239000006227 byproduct Substances 0.000 abstract description 3
- 150000001720 carbohydrates Chemical class 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 description 13
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 7
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 7
- 235000001785 ferulic acid Nutrition 0.000 description 7
- 229940114124 ferulic acid Drugs 0.000 description 7
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 7
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 7
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 6
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 6
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 description 6
- QAIPRVGONGVQAS-DUXPYHPUSA-N trans-caffeic acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-DUXPYHPUSA-N 0.000 description 6
- 239000003921 oil Substances 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 5
- ACEAELOMUCBPJP-UHFFFAOYSA-N (E)-3,4,5-trihydroxycinnamic acid Natural products OC(=O)C=CC1=CC(O)=C(O)C(O)=C1 ACEAELOMUCBPJP-UHFFFAOYSA-N 0.000 description 3
- NGSWKAQJJWESNS-ZZXKWVIFSA-M 4-Hydroxycinnamate Natural products OC1=CC=C(\C=C\C([O-])=O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-M 0.000 description 3
- DFYRUELUNQRZTB-UHFFFAOYSA-N Acetovanillone Natural products COC1=CC(C(C)=O)=CC=C1O DFYRUELUNQRZTB-UHFFFAOYSA-N 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 235000004883 caffeic acid Nutrition 0.000 description 3
- 229940074360 caffeic acid Drugs 0.000 description 3
- QAIPRVGONGVQAS-UHFFFAOYSA-N cis-caffeic acid Natural products OC(=O)C=CC1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-UHFFFAOYSA-N 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 229940074391 gallic acid Drugs 0.000 description 3
- 235000004515 gallic acid Nutrition 0.000 description 3
- PCMORTLOPMLEFB-UHFFFAOYSA-N sinapinic acid Natural products COC1=CC(C=CC(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-UHFFFAOYSA-N 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 2
- 239000000811 xylitol Substances 0.000 description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 2
- 229960002675 xylitol Drugs 0.000 description 2
- 235000010447 xylitol Nutrition 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002530 phenolic antioxidant Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a modification method of rice bran dietary fiber. The method comprises the following steps: pre-treating; carrying out enzymolysis on the defatted rice bran by using amylase and protease; high-temperature liquid water hydrolysis: solid-liquid separation and washing; the residue was dried and pulverized. The rice bran dietary fiber obtained by the method can be used for preparing hemicellulose and polyphenol of saccharides, can realize effective utilization of each component of the rice bran raw material, improves the added value of a byproduct, and improves the comprehensive utilization rate of the rice bran, thereby improving the economic benefit and the social benefit of an enterprise. The method has the characteristics of simple and convenient operation, environmental friendliness, low cost, high rice bran utilization rate, suitability for industrial production and the like.
Description
Technical Field
The invention relates to a modification method of rice bran dietary fiber, in particular to a modification method of rice bran dietary fiber for pre-extracting rice bran hemicellulose and polyphenol, and belongs to the technical field of grain processing and utilization.
Background
The rice bran is the most important byproduct in rice processing, and currently, the rice bran resource in China has the quantity of about 1000 ten thousand tons, so that the rice bran is a renewable resource with large quantity and wide range. According to statistics, most of rice bran in China is used as feed for poultry or discarded, and the utilization rate is very low. The main chemical components of rice bran include oil, starch, protein and dietary fiber. The rice bran is an important dietary fiber source, the dietary fiber has low energy content, has certain oil holding, water holding, oxidation resistance, cholesterol adsorption and other capabilities, has the functions of preventing constipation, adding satiety, reducing blood fat, reducing blood sugar and the like, and is a good resource. The dietary fiber in the rice bran, wherein 90% is insoluble dietary fiber, but the rest 10% is beneficial to human body to a higher extent. Therefore, the ratio of soluble dietary fiber to non-soluble dietary fiber is an important index for measuring the quality of the fiber. The dietary fiber is modified in order to increase the proportion of soluble dietary fiber, and the modification methods are physical methods, chemical methods and enzyme treatment methods. The proportion of soluble dietary fibre can be increased by these methods. In the aspect of fiber modification, separation methods include acid hydrolysis, alkali treatment and high-temperature hot water treatment. Among these methods, the high-temperature hot water treatment is advantageous in that cellulose and lignin are less lost, while hemicellulose, which is present in the form of saccharides, is more highly recovered. Compared with the acid hydrolysis method, the high-temperature hot water treatment enables the fibers to be hydrolyzed automatically, the generated byproducts are less, the operation is simpler and more convenient, and the step of intermediate neutralization treatment is omitted. In the hydrolysate rich in hemicellulose, phenolic antioxidants can be extracted, meanwhile, high value-added products can be generated, and the compounds obtained by hydrolysis can also be applied to the pharmaceutical and cosmetic industries.
The hemicellulose content of the rice bran dietary fiber is 20-25%, and the main component is pentosan which is one of the best raw materials of xylose, xylo-oligosaccharide, xylitol and arabinose. The rice bran dietary fiber is an excellent polyphenol source, and the content of ferulic acid is rich. At present, rice bran is used for preparing dietary fiber or polysaccharide by extracting cellulose. The two production modes have single target product and poor economic benefit. At present, no technical scheme for comprehensively utilizing rice bran for extracting hemicellulose/polyphenol and preparing dietary fiber is available.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a modification method of rice bran dietary fiber, wherein hemicellulose and polyphenol of rice bran are pre-extracted through high-temperature self-hydrolysis, so that a method for co-producing modified rice bran dietary fiber is achieved, the comprehensive utilization rate of the rice bran is improved, and the economic benefit and the social benefit of an enterprise are improved.
The technical scheme of the invention is as follows:
a modification method of rice bran dietary fiber is characterized by comprising the following steps:
(1) pretreatment: screening and removing impurities from the raw materials, crushing, sieving with a 40-60 mesh sieve, placing in a vegetable oil extraction solvent with the volume of 5-10 times of that of the raw materials, stirring and soaking for 1-6h, carrying out centrifugal separation, air-drying the precipitate, and removing the solvent to obtain defatted rice bran;
(2) performing enzymolysis on defatted rice bran with amylase and protease, namely adjusting the pH value to 6-7 with HCl, adding α -amylase of 300-600 u/g, stirring and reacting at 40-70 ℃ for 60-180 min, adding alkaline protease of 20-50 u/g, stirring and reacting at 40-60 ℃ and pH of 8.0-10.0 for 60-120 min, stirring at the speed of 100-120r/min, heating to 100 ℃ for reaction for 20min, performing suction filtration, washing precipitates with water for 3-5 times, and taking lower-layer precipitates;
(3) carrying out high-temperature liquid water hydrolysis on the wet material in a reactor, wherein the hydrolysis reaction temperature is 130-200 ℃, the reaction time is 20-240min, the solid-to-liquid ratio is 1:4-1:8, and the pH value at the end of the reaction is 2.5-5.0;
(4) performing solid-liquid separation and washing, and collecting filtrate to obtain an extracting solution, wherein the extracting solution comprises xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and acetic acid, and the extraction rate of the rice bran pentose is 10-40% for absolutely dry rice bran dietary fiber raw materials;
(5) and drying and crushing the rice bran dietary fiber residue after solid-liquid separation to obtain the rice bran dietary fiber. On the basis of the scheme, in the step (4), the product in the step (3) is subjected to liquid-solid separation in a continuous squeezing mode, filtrate is collected to prepare extracting solution, and residues are collected for later use.
The continuous squeezing mode and the multi-section countercurrent washing technology adopt the known and disclosed technical scheme, and can be spiral extrusion, roller type squeezing or vacuum filtration, etc., the first section is washed and squeezed by the second section of filtrate, the second section is washed and squeezed by the third section of filtrate, and so on, the last section is washed and squeezed by clear water, the first section of concentrated filtrate is collected to prepare extracting solution, and the last section of residue is collected for standby.
The method for modifying the rice bran dietary fiber, which is disclosed by the claims 1-2, is characterized in that: the pentose extraction rate of the rice bran extract obtained by the steps 1 to 4 is 10 to 40 percent, the pH value of the extract is 2.5 to 5.0, and the components comprise xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and acetic acid.
The product obtained by the modification method of the rice bran dietary fiber is rice bran extract obtained by rice bran pretreatment, rice bran enzymolysis and liquid-solid separation in the steps (1) to (4), and has the advantages that the rice bran pentose extraction rate is 20-40%, the pH value of the extract is 2.5-5.0, and the components comprise xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and acetic acid.
The invention has the beneficial effects that:
(1) the hemicellulose extract comprises xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and the like, and can be used for preparing food additives and chemical products such as xylo-oligosaccharide, xylose, xylitol, arabinose, polyphenol and the like.
(2) The modification method of the rice bran dietary fiber provided by the invention greatly improves the performance of the rice bran dietary fiber.
(3) The technical scheme of comprehensive utilization of rice bran for extracting hemicellulose/polyphenol and preparing rice bran dietary fiber provided by the invention improves the utilization rate of rice bran and also improves the economic benefit and social benefit of enterprises.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below. Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention.
Example 1
A modification method of rice bran dietary fiber is characterized by comprising the following steps:
(1) pretreatment: screening and removing impurities from the raw materials, crushing, sieving with a 40-60 mesh sieve, placing in a vegetable oil extraction solvent with the volume of 5 times, stirring and soaking for 1h, performing centrifugal separation, air drying the precipitate, and removing the solvent to obtain defatted rice bran;
(2) performing enzymolysis on defatted rice bran with amylase and protease, adjusting pH to 6.5 with HCl, adding α -amylase 600u/g, stirring at 70 deg.C for 180min, adding alkaline protease 50u/g, stirring at 60 deg.C and pH 10.0 for 120min, stirring at 120r/min, heating to 100 deg.C for 20min, vacuum filtering, washing with water for 5 times, and collecting the lower layer precipitate;
(3) hydrolyzing the wet material in a reactor, wherein the hydrolysis reaction temperature is 130 ℃, the reaction time is 240min, and the solid-to-liquid ratio is 1:4, the reaction end pH is 5.0;
(4) performing liquid-solid separation and countercurrent washing on the hydrolysate by using a three-section vacuum filter, washing and squeezing the hydrolysate by using a second section of filtrate in the first section, washing and squeezing the hydrolysate by using a third section of filtrate in the second section, washing and squeezing the hydrolysate by using water in the third section, and collecting the concentrated filtrate in the first section to prepare an extracting solution, wherein the extraction rate of the pentose of the rice bran is 35 percent, and the components of the pentose of the rice bran comprise xylo-oligosaccharide, xylose, arabinose, glucose, uronic acid, polyphenol and acetic acid; the total phenol content is 5.5%, and the polyphenols include ferulic acid, gallic acid, sinapic acid, p-coumaric acid and caffeic acid, wherein ferulic acid is the main polyphenol species; and collecting the residue in the third stage, wherein the dryness of the residue is not less than 35%.
(5) And (3) drying the collected rice bran dietary fiber residues by hot air at the drying temperature of 50 ℃, and crushing to obtain the modified rice bran dietary fiber, wherein the water holding capacity is 5.84g/g, the expansibility is 0.66mL/g, and the oil holding capacity is 3.58g/g.
Example 2
A modification method of rice bran dietary fiber is characterized by comprising the following steps:
(1) pretreatment: screening and removing impurities from the raw materials, crushing, sieving with a 40-60 mesh sieve, then placing in a vegetable oil extraction solvent with the volume of 10 times, stirring and soaking for 6 hours, carrying out centrifugal separation, air-drying the precipitate, and removing the solvent to obtain defatted rice bran;
(2) performing enzymolysis on defatted rice bran with amylase and protease, adjusting pH to 6.5 with HCl, adding α -amylase 300u/g, stirring at 40 deg.C for 60min, adding alkaline protease 20u/g, stirring at 40 deg.C and pH 8.0 for 60min, stirring at 100r/min, heating to 100 deg.C for 20min, vacuum filtering, washing with water for 3 times, and collecting the lower layer precipitate;
(3) carrying out high-temperature hot water hydrolysis on the wet material in a reactor, wherein the hydrolysis reaction temperature is 200 ℃, the reaction time is 20min, the solid-to-liquid ratio is 1:8, and the pH value at the end of the reaction is 2.5;
(4) performing liquid-solid separation and countercurrent washing on the hydrolysate by using a three-section vacuum filter, washing and squeezing the hydrolysate by using the second section of filtrate in the first section, washing and squeezing the hydrolysate by using the third section of filtrate in the second section, washing and squeezing the hydrolysate by using water in the third section, and collecting the concentrated filtrate in the first section to prepare an extracting solution, wherein the extraction rate of the pentose of the rice bran is 34 percent, and the components of the pentose of the rice bran comprise xylo-oligosaccharide, xylose, arabinose, glucose, uronic acid, polyphenol and acetic acid; the total phenol content is 5.6%, and the polyphenols include ferulic acid, gallic acid, sinapic acid, p-coumaric acid and caffeic acid, wherein ferulic acid is the main polyphenol species; collecting the third stage residue, wherein the dryness of the residue is not lower than 36%;
(5) and (3) drying the collected rice bran dietary fiber residues by hot air at the drying temperature of 55 ℃, and crushing to obtain the rice bran dietary fiber. The water holding capacity is 5.64g/g, the expansibility is 0.63mL/g, and the oil holding capacity is 3.66g/g.
Example 3
A modification method of rice bran dietary fiber is characterized by comprising the following steps:
(1) pretreatment: screening and removing impurities from the raw materials, crushing, sieving with a 40-60 mesh sieve, placing in a vegetable oil extraction solvent with the volume of 6 times, stirring and soaking for 2 hours, carrying out centrifugal separation, air-drying the precipitate, and removing the solvent to obtain defatted rice bran;
(2) performing enzymolysis on defatted rice bran with amylase and protease, adjusting pH to 6.6 with HCl, adding α -amylase 400u/g, stirring at 50 deg.C for 90min, adding alkaline protease 30u/g, stirring at 50 deg.C and pH 8.5 for 80min, stirring at 110r/min, heating to 100 deg.C for 20min, vacuum filtering, washing with water for 4 times, and collecting the lower layer precipitate;
(3) carrying out high-temperature liquid water hydrolysis on the wet material in a reactor, wherein the hydrolysis reaction temperature is 140 ℃, the reaction time is 60min, the solid-liquid ratio is 1:5, and the pH value at the end of the reaction is 3.0;
(4) performing liquid-solid separation and countercurrent washing on the hydrolysate by using a three-section vacuum filter, washing and squeezing the hydrolysate by using the second section of filtrate in the first section, washing and squeezing the hydrolysate by using the third section of filtrate in the second section, washing and squeezing the hydrolysate by using water in the third section, and collecting the concentrated filtrate in the first section to prepare an extracting solution, wherein the extraction rate of the pentose of the rice bran is 36 percent, and the components of the pentose of the rice bran comprise xylo-oligosaccharide, xylose, arabinose, glucose, uronic acid, polyphenol and acetic acid; the total phenol content is 5.8%, and the polyphenols include ferulic acid, gallic acid, sinapic acid, p-coumaric acid and caffeic acid, wherein ferulic acid is the main polyphenol species; collecting the third stage residue, wherein the dryness of the residue is not lower than 37%;
(5) and (3) drying the collected rice bran dietary fiber residues by hot air at the drying temperature of 60 ℃, and crushing to obtain the rice bran dietary fiber. The water holding capacity is 5.58g/g, the expansibility is 0.68mL/g, and the oil holding capacity is 3.59 g/g.
Claims (4)
1. A modification method of rice bran dietary fiber is characterized by sequentially comprising the following steps:
(1) pretreatment: screening and removing impurities from the raw materials, crushing, sieving with a 40-60 mesh sieve, placing in a vegetable oil extraction solvent with the volume of 5-10 times of that of the raw materials, stirring and soaking for 1-6h, carrying out centrifugal separation, air-drying the precipitate, and removing the solvent to obtain defatted rice bran;
(2) performing enzymolysis on defatted rice bran with amylase and protease, namely adjusting the pH value to 6-7 with HCl, adding α -amylase of 300-600 u/g, stirring and reacting at 40-70 ℃ for 60-180 min, adding alkaline protease of 20-50 u/g, stirring and reacting at 40-60 ℃ and pH of 8.0-10.0 for 60-120 min, stirring at the speed of 100-120r/min, heating to 100 ℃ for reaction for 20min, performing suction filtration, washing precipitates with water for 3-5 times, and taking lower-layer precipitates;
(3) carrying out high-temperature liquid water hydrolysis on the wet material in a reactor: the hydrolysis reaction temperature is 130-200 ℃, the reaction time is 20-240min, the solid-to-liquid ratio is 1:4-1:8, and the pH value at the end of the reaction is 2.5-5.0;
(4) performing solid-liquid separation and washing, and collecting filtrate to obtain an extracting solution, wherein the extracting solution comprises xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and acetic acid, and the extraction rate of the rice bran pentose is 10-40% for absolutely dry rice bran dietary fiber raw materials;
(5) and drying and crushing the rice bran dietary fiber residue after solid-liquid separation to obtain the rice bran dietary fiber.
2. The method for modifying rice bran dietary fiber according to claim 1, wherein the method comprises the following steps: and (4) performing liquid-solid separation on the product obtained in the step (2) by adopting a continuous squeezing mode, collecting filtrate to prepare extracting solution, and collecting residues for later use.
3. The method for modifying the rice bran dietary fiber, which is disclosed by the claims 1-2, is characterized in that: the pentose extraction rate of the rice bran extract obtained by the steps 1 to 4 is 10 to 40 percent, the pH value of the extract is 2.5 to 5.0, and the components comprise xylo-oligosaccharide, xylose, arabinose, glucose, polyphenol, uronic acid and acetic acid.
4. The method for modifying rice bran dietary fiber according to claim 1, wherein the method comprises the following steps: and (5) drying in a vacuum drying mode or a hot air drying mode, wherein the drying temperature is lower than 60 ℃.
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| CN101558844A (en) * | 2008-11-27 | 2009-10-21 | 江南大学 | Preparation of dietary fiber from rice bran particles and application of the same in sausage |
| CN101613970A (en) * | 2009-06-09 | 2009-12-30 | 上海士林纤维材料有限公司 | Bagasse dissolving pulp pulping process of preextraction hemicellulose and products thereof |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN107212425A (en) * | 2017-05-09 | 2017-09-29 | 湖北工业大学 | A kind of method for extracting rice bran dietary fiber |
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| CN101558844A (en) * | 2008-11-27 | 2009-10-21 | 江南大学 | Preparation of dietary fiber from rice bran particles and application of the same in sausage |
| CN101613970A (en) * | 2009-06-09 | 2009-12-30 | 上海士林纤维材料有限公司 | Bagasse dissolving pulp pulping process of preextraction hemicellulose and products thereof |
| CN105361187A (en) * | 2015-09-28 | 2016-03-02 | 华中农业大学 | Modified cereal dietary fiber and preparation method thereof |
| CN107212425A (en) * | 2017-05-09 | 2017-09-29 | 湖北工业大学 | A kind of method for extracting rice bran dietary fiber |
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