CN111004830A - Method for releasing hydrogen influenced by hydrogen on cells in cell experiment - Google Patents
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Abstract
The invention discloses a method for releasing hydrogen influenced by hydrogen on cells in a cell experiment, which comprises the following steps: (1) putting metal into a cell culture plate, adding liquid which reacts with the metal to generate hydrogen, and simultaneously, the liquid level of the added liquid is required to be lower than the bottom surface of the cell embedding dish corresponding to the liquid level; (2) placing the cell embedding dish on a cell culture plate corresponding to the cell embedding dish, and planting the cells to be researched on the cell embedding dish; (3) the cover of the cell culture plate is covered and put into a cell culture box for cell culture, and the influence of hydrogen on the cells to be studied is detected after the cells are cultured for a certain time. The method can continuously and stably release hydrogen in situ around the cells, efficiently acts with the cells, is convenient for calculating the release amount of the hydrogen subsequently, ensures the accuracy of the experiment, and only allows the hydrogen to enter the cell culture solution without causing the change of other components in the culture solution.
Description
Technical Field
The invention relates to a method for releasing hydrogen influenced by hydrogen on cells in a cell experiment, in particular to a method for releasing hydrogen influenced by hydrogen on cells in a cell experiment, and belongs to the technical field of application of hydrogen release methods.
Background
In the current cell experiment, some people adopt a method of introducing hydrogen with corresponding concentration into an incubator. The method can maintain the stability of hydrogen concentration in the incubator, but the experiment needs a separate incubator for cell culture, the amount of hydrogen acting on cells cannot be determined, and the hydrogen utilization rate is low (see a Chinese patent with the application number of 201710932196.1, which discloses the application of hydrogen in preparing a preparation for preventing and treating leucoderma, and a Chinese patent with the application number of 201811396038.X, which discloses a treatment specification for treating dermal microvascular endothelial cell reperfusion injury by hydrogen).
Some people adopt a method of cell culture by adding hydrogen-rich water to a complete culture solution, but the addition of hydrogen-rich water lowers the osmotic pressure value of the culture solution, causes cell damage due to low osmotic pressure when the hydrogen-rich water content reaches a certain value, lowers the experimental accuracy, and releases hydrogen-rich water into the air soon upon pouring out from a storage device (see the journal "MOLECULAR MEDICAL REPORTS" in 6.2018, page 2182-2190, which discloses "efficacy of hydrogen-rich saline and in combination with PI3K inhibitor in non-small cell lung cancer" (hydrogen-rich water alone and efficacy of treating non-small cell lung cancer in combination with PI3K inhibitor) ".
Disclosure of Invention
The present invention is directed to solving the above problems and providing a method for releasing hydrogen gas by the effect of hydrogen gas on cells in a cell experiment.
The purpose of the invention is realized by the following technical scheme:
the invention provides a method for releasing hydrogen influenced by hydrogen on cells in a cell experiment, which comprises the following steps:
(1) metal and liquid put in cell culture plate: putting metal into a cell culture plate, adding liquid which reacts with the metal to generate hydrogen, and simultaneously, the liquid level of the added liquid is required to be lower than the bottom surface of the cell embedding dish corresponding to the liquid level;
(2) cell-embedded dish placement: placing the cell embedding dish on a cell culture plate corresponding to the cell embedding dish, and planting the cells to be researched on the cell embedding dish; planting the cells to be researched, namely adding the cells to be researched and a cell culture solution into a cell embedding dish;
(3) the cover of the cell culture plate is covered and put into a cell culture box for cell culture, and the influence of hydrogen on the cells to be studied is detected after the cells are cultured for a certain time.
Preferably, the liquid and the metal in the step (1) are both required to be sterilized for use.
Preferably, the metal in step (1) is magnesium, zinc, sodium, potassium, calcium or an alloy thereof, and hydrogen can be generated relatively stably. More preferably, the metal is magnesium, the reaction speed of the metal and the solution is moderate, the hydrogen release speed is stable and moderate, and the effect is best.
More preferably, the metal is sheet-shaped, so that the liquid level does not exceed the bottom surface of the embedding dish while the metal is immersed, the liquid level is as low as possible, otherwise, the liquid in the culture plate is easily attached to the bottom surface of the embedding dish in the process of putting the culture plate into the incubator.
Preferably, the liquid in step (1) is a weakly alkaline solution or a neutral solution or an acidic solution.
More preferably, the weak alkaline solution comprises simulated body fluid, cell culture fluid; the neutral solution comprises water and a sodium chloride solution; the acid solution comprises 4-hydroxyethyl piperazine ethanesulfonic acid and hydrochloric acid solution.
The steps (1) and (2) need to be operated in a sterile super clean bench, all operations are in a sterile environment, and the related reagents and instruments are sterilized.
And (2) after the liquid added in the step (1) is sterilized, placing the sterilized liquid in a sterilized centrifugal tube, and transferring the liquid by using a sterilized liquid transfer device and a liquid transfer device gun head.
In the step (2), the cell embedding dish and the culture plate need workers to wear sterile protective gloves, so that the cells embedded in the dish are prevented from being polluted.
The cell embedding dishes in the step (2) are independent dishes with different sizes, and the cell embedding dishes can be matched with 6-well, 12-well and 24-well cell culture plates and the like, and can be selected according to needs.
And (4) the cover in the step (3) is an original cover matched with the corresponding cell culture plate.
And (4) in the step (3), each independent space in the cell culture box is a clean sterile environment.
Compared with the prior art, the invention has the following beneficial effects:
the method can continuously and stably release hydrogen in situ around the cells, efficiently acts with the cells, is convenient for calculating the release amount of the hydrogen subsequently, ensures the accuracy of the experiment, only allows the hydrogen to enter the cell culture solution, does not cause the change of other components in the culture solution, can carry out gradient experiments of different hydrogen concentrations in the same incubator, does not need a plurality of incubators with different hydrogen concentrations to carry out cell culture independently, saves manpower and material resources, and is suitable for popularization and use.
In the method of the present invention, only hydrogen gas enters the cell-embedding dish, and the liquid in the culture plate does not flow back into the cell-embedding dish, so that the osmotic pressure of the culture solution is not reduced, and cells can be cultured in the normal culture solution.
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Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a flow chart of the operational steps of the present invention;
FIG. 2 shows SKOV after culturing in example 13The OD value of the cells;
FIG. 3 is an OD value of U2-OS cells after culturing in example 1;
FIG. 4 shows SKOV after culturing in example 13OD value of the cells.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be obvious to those skilled in the art that various changes and modifications can be made without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1:
a method for releasing hydrogen gas in a cell experiment by hydrogen gas influence on cells, as shown in fig. 1, the hydrogen gas releasing method comprises the following steps:
(1) metal and liquid put in cell culture plate: six high-purity magnesium sheets with the length of 12 mm, the width of 10 mm and the thickness of 0.3 mm are averagely placed into three holes of a 6-hole cell culture plate, 0.5 ml of simulated body fluid is added, the liquid level of the added simulated body fluid is ensured to be lower than the bottom surface of a cell embedding dish corresponding to the liquid level, and 0.5 ml of simulated body fluid is also respectively added into the other three holes of the 6-hole cell culture plate without the magnesium sheets to serve as a control group.
(2) Cell-embedded dish placement: cell-embedded dishes matched to 6-well cell culture plates were placed on 6-well cell culture plates, and 2 x 10 cells were plated on each of the cell-embedded dishes5SKOV3A cell.
(3) Covering the cover of the cell culture plate and placing the cell culture plate into a cell culture box, wherein the cell culture box adopts a plurality of same independent spaces which are placed in layers, and the culture plate covered with the cover is placed under the condition of 5% CO2Cells were cultured in a cell incubator at 37 ℃ and hydrogen vs. SKOV was detected by cck8 assay after 48 hours of culture3The effect of cell proliferation.
And (2) sterilizing the liquid and the metal in the step (1) for later use.
The steps (1) and (2) need to be operated in a sterile super clean bench, all operations are in a sterile environment, and the related reagents and instruments are sterilized.
And (2) after the liquid added in the step (1) is sterilized, placing the sterilized liquid in a sterilized centrifugal tube, and transferring the liquid by using a sterilized liquid transfer device and a liquid transfer device gun head.
In the step (2), the cell embedding dish and the culture plate need workers to wear sterile protective gloves, so that the cells embedded in the dish are prevented from being polluted.
And (4) the cover in the step (3) is an original cover matched with the corresponding cell culture plate.
And (4) in the step (3), each independent space in the cell culture box is a clean sterile environment.
Experimental results as shown in fig. 2, it can be seen from the results that the addition of hydrogen significantly inhibited the proliferation of SKOV3 cells.
Example 2:
a method for hydrogen release in a cell experiment for hydrogen influencing cells, the hydrogen release method comprising the steps of:
(1) metal and liquid put in cell culture plate: six high-purity magnesium sheets with the length of 12 mm, the width of 10 mm and the thickness of 0.3 mm are averagely placed into three holes of a 6-hole cell culture plate, 0.5 ml of simulated body fluid is added, the liquid level of the added simulated body fluid is ensured to be lower than the bottom surface of a cell embedding dish corresponding to the liquid level, and 0.5 ml of simulated body fluid is also respectively added into the other three holes of the 6-hole cell culture plate without the magnesium sheets to serve as a control group.
(2) Cell-embedded dish placement: cell-embedded dishes matched to 6-well cell culture plates were placed on 6-well cell culture plates, and 2 x 10 cells were plated on each of the cell-embedded dishes5U2-OS cells.
(3) Covering the cover of the cell culture plate and placing the cell culture plate into a cell culture box, wherein the cell culture box adopts a plurality of same independent spaces which are placed in layers, and the culture plate covered with the cover is placed under the condition of 5% CO2Cells were cultured in a cell incubator at 37 ℃ and the effect of hydrogen on proliferation of U2-OS cells was examined by cck8 assay after 48 hours of culture.
And (2) sterilizing the liquid and the metal in the step (1) for later use.
The steps (1) and (2) need to be operated in a sterile super clean bench, all operations are in a sterile environment, and the related reagents and instruments are sterilized.
And (2) after the liquid added in the step (1) is sterilized, placing the sterilized liquid in a sterilized centrifugal tube, and transferring the liquid by using a sterilized liquid transfer device and a liquid transfer device gun head.
In the step (2), the cell embedding dish and the culture plate need workers to wear sterile protective gloves, so that the cells embedded in the dish are prevented from being polluted.
And (4) the cover in the step (3) is an original cover matched with the corresponding cell culture plate.
And (4) in the step (3), each independent space in the cell culture box is a clean sterile environment.
The experimental results are shown in fig. 3, from which it can be seen that the addition of hydrogen significantly inhibited the proliferation of U2-OS cells.
Example 3:
a method for hydrogen release in a cell experiment for hydrogen influencing cells, the hydrogen release method comprising the steps of:
(1) metal and liquid put in cell culture plate: putting six high-purity magnesium sheets with the length of 12 mm, the width of 10 mm and the thickness of 0.3 mm into three holes of a 6-hole cell culture plate on average, adding 0.5 ml of 4-hydroxyethylpiperazine ethanesulfonic acid to ensure that the liquid level of the added 4-hydroxyethylpiperazine ethanesulfonic acid is lower than the bottom surface of a corresponding cell embedding dish, and respectively adding 0.5 ml of 4-hydroxyethylpiperazine ethanesulfonic acid into the other three holes of the 6-hole cell culture plate without the magnesium sheets as a control group.
(2) Cell-embedded dish placement: cell-embedded dishes matched to 6-well cell culture plates were placed on 6-well cell culture plates, and 2 x 10 cells were plated on each of the cell-embedded dishes5SKOV3A cell.
(3) Covering the cover of the cell culture plate and placing the cell culture plate into a cell culture box, wherein the cell culture box adopts a plurality of same independent spaces which are placed in layers, and the culture plate covered with the cover is placed under the condition of 5% CO2Cells were cultured in a cell incubator at 37 ℃ and hydrogen vs. SKOV was detected by cck8 assay after 48 hours of culture3The effect of cell proliferation.
And (2) sterilizing the liquid and the metal in the step (1) for later use.
Preferably, the step (1) and the step (2) need to be operated in a sterile super clean bench, all operations are in a sterile environment, and the related reagents and instruments are sterilized.
And (2) after the liquid added in the step (1) is sterilized, placing the sterilized liquid in a sterilized centrifugal tube, and transferring the liquid by using a sterilized liquid transfer device and a liquid transfer device gun head.
In the step (2), the cell embedding dish and the culture plate need workers to wear sterile protective gloves, so that the cells embedded in the dish are prevented from being polluted.
And (4) the cover in the step (3) is an original cover matched with the corresponding cell culture plate.
And (4) in the step (3), each independent space in the cell culture box is a clean sterile environment.
Experimental results as shown in fig. 4, it can be seen from the results that the addition of hydrogen significantly inhibited the proliferation of SKOV3 cells.
Example 4
A method of hydrogen evolution in a cell experiment, the hydrogen evolution method being substantially the same as in example 1 except that: the metal adopted in the embodiment is pure zinc sheet, and the liquid is 4-hydroxyethyl piperazine ethanesulfonic acid.
The experimental results show that hydrogen can be stably released, so that the proliferation of the research cell SKOV3 is obviously lower than that of a control group without hydrogen release.
Example 5
A method of hydrogen evolution in a cell experiment, the hydrogen evolution method being substantially the same as in example 2 except that: the metal used in this example is magnesium-zinc alloy, and the liquid is a cell culture solution of the same formulation as the cultured cells.
The experimental result shows that hydrogen can be stably released, and the proliferation of U2-OS cells is inhibited.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (5)
1. A method for hydrogen release in a cell assay for the effect of hydrogen on cells, comprising: the method comprises the following steps:
(1) metal and liquid put in cell culture plate: putting metal into a cell culture plate, adding liquid which reacts with the metal to generate hydrogen, and simultaneously, the liquid level of the added liquid is required to be lower than the bottom surface of the cell embedding dish corresponding to the liquid level;
(2) cell-embedded dish placement: placing the cell embedding dish on a cell culture plate corresponding to the cell embedding dish, and planting the cells to be researched on the cell embedding dish;
(3) the cover of the cell culture plate is covered and put into a cell culture box for cell culture, and the influence of hydrogen on the cells to be studied is detected after the cells are cultured for a certain time.
2. Method of hydrogen release in a cell experiment according to claim 1, characterized in that: and (2) sterilizing the liquid and the metal in the step (1) for later use.
3. Method of hydrogen release in a cell experiment according to claim 1, characterized in that: in the step (1), the metal is magnesium, zinc, sodium, potassium, calcium or alloy thereof.
4. Method of hydrogen release in a cell experiment according to claim 1, characterized in that: the liquid in the step (1) is a weak alkaline solution, a neutral solution or an acidic solution.
5. Method of hydrogen release in cellular experiments with hydrogen influence on cells according to claim 4, characterized in that: the weak alkaline solution comprises simulated body fluid and cell culture solution; the neutral solution comprises water and a sodium chloride solution; the acid solution comprises 4-hydroxyethyl piperazine ethanesulfonic acid and hydrochloric acid solution.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113444637A (en) * | 2021-01-28 | 2021-09-28 | 潍坊医学院 | Hydrogen culture experiment system and method for researching endothelial progenitor cell damage repair |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2732750Y (en) * | 2004-10-27 | 2005-10-12 | 贝瑞特生物技术（郑州）有限责任公司 | Culture utensil for anaerobic culture |
| CN204803324U (en) * | 2015-07-02 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture board of area embedding ware |
| CN206538417U (en) * | 2017-03-10 | 2017-10-03 | 甘肃农业大学 | A kind of easy culture dish for anaerobe |
| CN107281214A (en) * | 2017-06-06 | 2017-10-24 | 北京工业大学 | Hydrogen molecule liquid/gas is preparing the application in suppressing tumor stem cell medicine |
-
2019
- 2019-12-12 CN CN201911271113.4A patent/CN111004830A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2732750Y (en) * | 2004-10-27 | 2005-10-12 | 贝瑞特生物技术（郑州）有限责任公司 | Culture utensil for anaerobic culture |
| CN204803324U (en) * | 2015-07-02 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture board of area embedding ware |
| CN206538417U (en) * | 2017-03-10 | 2017-10-03 | 甘肃农业大学 | A kind of easy culture dish for anaerobe |
| CN107281214A (en) * | 2017-06-06 | 2017-10-24 | 北京工业大学 | Hydrogen molecule liquid/gas is preparing the application in suppressing tumor stem cell medicine |
Non-Patent Citations (3)
| Title |
|---|
| 吴茂江等: "《走进化学探究室》", 30 November 2011 * |
| 毛丽贺等: "镁钙锌合金在人体模拟体液中的腐蚀行为", 《材料导报》 * |
| 王新印等: "镁及其合金腐蚀析氢的研究现状", 《装备环境工程》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113444637A (en) * | 2021-01-28 | 2021-09-28 | 潍坊医学院 | Hydrogen culture experiment system and method for researching endothelial progenitor cell damage repair |
| CN113444637B (en) * | 2021-01-28 | 2022-08-05 | 潍坊医学院 | Hydrogen culture experiment system and method for researching endothelial progenitor cell damage repair |
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