CN110988159A - Method for measuring contents of multiple components of Jingyaokang capsule - Google Patents
Method for measuring contents of multiple components of Jingyaokang capsule Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
- G01N2030/047—Standards external
Abstract
The invention provides a neck and waist rehabilitation capsule multi-component content determination method which comprises the following steps of S1, preparing a mixed reference substance solution, respectively and precisely weighing strychnine, α -Chamaecyparis obtusa alkali, periplocin, 11-carbonyl- β -boswellic acid, zedoary turmeric bicyclenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone, dissolving the weighed materials in a solvent to obtain a mixed reference substance solution for later use, S2, preparing a test substance solution, taking the neck and waist rehabilitation capsule, adding the solvent for extraction, filtering, taking a filtrate to obtain a test substance solution for later use, and S3, performing content determination, respectively and precisely sucking the reference substance solution and the test substance solution, injecting a liquid chromatograph, and determining to obtain the neck and waist rehabilitation capsule multi-component content determination method, wherein the contents of 10 chemical components such as strychnine, α -Chamaecyparis obtusin, 11-carbonyl- β -boswellic acid and naringin in the neck and waist rehabilitation capsule can be better controlled in quality.
Description
Technical Field
The invention relates to the field of medicine detection, and particularly relates to a method for measuring the content of multiple components of a Jingyaokang capsule.
Background
The Chinese patent medicine neck and waist health-care capsule is composed of ten medicines of prepared nux vomica, lycopodium clavatum, cortex periplocae, frankincense, myrrh, safflower and the like; has the effects of relaxing muscles and tendons, activating collateral flow, promoting blood circulation, removing blood stasis, relieving swelling and pain, and can be used for treating fracture blood stasis, swelling and pain, fracture convalescence, arthralgia due to kidney deficiency and dampness, proliferative spondylitis, prolapse of lumbar intervertebral disc, etc.
The existing quality standard of the Jingyaokang capsule mainly comprises the thin-layer identification of safflower, cortex periplocae, radix stephaniae tetrandrae and radix achyranthis bidentatae and the content measurement of strychnine in the semen strychni preparata, only controls the content measurement of one component of one medicine and the qualitative identification of a few components, and is difficult to reflect the quality of a product on the whole or control the quality of the product on the whole. The invention patent with publication number CN108169386A discloses a method for constructing an HPLC characteristic spectrum of a Jingyaokang capsule, which can only evaluate the quality of the Jingyaokang capsule as a whole. The invention patent with the publication number of CN109270203A discloses a method for constructing a characteristic spectrum of an active ingredient of a Jingyaokang capsule and a quality detection method of the Jingyaokang capsule, which only effectively monitor the active ingredient strychnine and/or naringin, but still detect various ingredients of the Jingyaokang capsule. Therefore, the method for simply and accurately carrying out quantitative analysis on various index components in the Jingyaokang capsule is a technical problem.
Disclosure of Invention
The invention aims to solve the problems, and provides a method for measuring the contents of 10 chemical components such as strychnine, α -Chamaecyparis obtusa alkali, periplocin, 11-carbonyl- β -boswellic acid, naringin and the like in a Jingyaokang capsule, so that the quality of the Jingyaokang capsule can be better controlled.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for measuring the content of multiple components of a Jingyaokang capsule comprises the following steps:
s1, preparation of a mixed reference solution:
accurately weighing strychnine, α -chamaecyparis, periplocin, 11-carbonyl- β -boswellic acid, zedoary dicyclo ketene, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone, respectively, dissolving in solvent to obtain mixed reference solution for use.
S2, preparation of a test solution:
extracting JINGYAOKANG Capsule with solvent, filtering, and collecting filtrate to obtain test solution.
S3, content determination:
precisely sucking the reference solution and the sample solution, respectively, injecting into a liquid chromatograph, and measuring.
Preferably, the solvent is methanol.
Preferably, in step S1, strychnine, α -sabcomene, periplocin, 11-carbonyl- β -boswellic acid, curcumenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline, and β -ecdysterone are precisely weighed, placed in the same 25mL measuring flask, diluted to scale with 70% methanol, and shaken to obtain a mixed reference solution with the mass concentrations of the above 10 components being 85.6 μ g/mL, 339.4 μ g/mL, 56.3 μ g/mL, 285.2 μ g/mL, 48.9 μ g/mL, 73.6 μ g/mL, 24.8 μ g/mL, 36.2 μ g/mL, 59.3 μ g/mL, and 285.6 μ g/mL in sequence.
Preferably, in step S2, precisely weighing 0.5g of the content of the Jingyaokang capsule, adding 70% methanol to a constant volume of 50mL, sealing, weighing, performing ultrasonic treatment for 10min, weighing again, supplementing the lost mass with 70% methanol, shaking up, filtering, and collecting the subsequent filtrate to obtain the test solution.
Preferably, the sonication power is 200W and the frequency is 50 kHz.
Preferably, during the gradient elution:
the volume fraction of the mobile phase A is changed from 10% to 25% in 0-25 min;
changing the volume fraction of the mobile phase A from 25% to 65% in 25-45 min;
and (4) changing the volume fraction of the mobile phase A from 65% to 85% in 45-60 min.
Preferably, in step S3, the detection wavelength is: detection wavelength: 403nm in 0-12 min; 275nm in 12-18 min; 230nm for 18-45 min; 282nm in 45-60 min.
Preferably, in step S3, the detection condition includes: the chromatographic column is Kromasil C18; performing gradient elution by using acetonitrile-methanol aqueous solution with the volume ratio of 2: 1 as a mobile phase A and glacial acetic acid solution with the volume fraction of 0.05% as a mobile phase B, wherein the flow rate of the mobile phase is 1.0 mL/min; the column temperature was 25 ℃.
Preferably, in step S3, the sample size is 10 μ L.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
1. the HPLC method is adopted to carry out quality control research on the Jingyakang capsules, the contents of 10 chemical components such as strychnine, α -hinokitione, periplocin, 11-carbonyl- β -boswellic acid, naringin and the like in the Jingyakang capsules are measured, the composition and proportion change conditions of main effective components in the Jingyakang capsules can be comprehensively inspected, the time is saved, and the detection accuracy is improved.
The method can simultaneously carry out quantitative detection on 10 chemical components such as strychnine, α -sabelline, periplocin, 11-carbonyl- β -boswellic acid, naringin and the like in the effective components of the Jingyaokang capsule, can comprehensively inspect the composition and proportion change conditions of the main effective components in the Jingyaokang capsule, reduces analysis steps and reduces component change and determination errors possibly caused by a sample pretreatment link.
2. The determination method provided by the invention adopts a high performance liquid chromatogram method to perform qualitative and quantitative detection on the Jingyaokang capsule, and acetonitrile-methanol aqueous solution and glacial acetic acid solution are selected as mobile phases according to the characteristics of the effective components of the Jingyaokang capsule, and the proportion of the phases in the mobile phases is continuously changed according to the program to perform gradient elution, so that the separation degree between chromatographic peaks of the components is good, the interference is small, the peak shape is good, the base line is stable, and the acid solution with lower concentration is beneficial to protecting a chromatographic column.
3. The method for detecting the absorption wavelength of the components has large difference of the maximum absorption wavelength of the components and is difficult to show the maximum absorption wavelength of the components at the same wavelength, so that the method for detecting the absorption wavelength of the components adopts a multi-wavelength simultaneous detection mode to carry out detection, and the detection wavelength of the components is optimized on the basis.
4. The determination method provided by the invention has the advantages of simplicity, convenience, stability, high precision, good reproducibility and easiness in mastering.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Materials:
the apparatus used in the examples of the invention is shown in table 1 below.
TABLE 1 Instrument
The embodiment of the invention uses medicines and reagents:
strychnine, α -selagineline, 11-carbonyl- β -boswellic acid, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone reference substances (China institute for food and drug assay, batches: 110525-;
periplocin and curcumenone reference substance (Kyoto Biotech Co., Ltd., lot numbers: 201902114, 201902115, purity: 98%);
the methanol and the acetonitrile are chromatographically pure, and other reagents are analytically pure.
Example 1
A method for measuring the content of multiple components of a Jingyaokang capsule comprises the following steps:
s1, preparation of a mixed reference solution:
accurately weighing strychnine, α -Chamaecyparis obtusa alkali, periplocin, 11-carbonyl- β -boswellic acid, zedoary dicyclenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone as reference substances, placing the reference substances in a same 25mL measuring flask, diluting the reference substances to scale with 70% methanol, and shaking uniformly to obtain mixed reference substance solutions with the mass concentrations of the above 10 components being 85.6 μ g/mL, 339.4 μ g/mL, 56.3 μ g/mL, 285.2 μ g/mL, 48.9 μ g/mL, 73.6 μ g/mL, 24.8 μ g/mL, 36.2 μ g/mL, 59.3 μ g/mL and 285.6 μ g/mL in sequence.
S2, preparation of a test solution:
precisely weighing 0.5g of neck and waist health capsule content, adding 70% methanol to constant volume to 50mL, sealing, weighing, ultrasonically treating for 10min, weighing again, supplementing lost mass with 70% methanol, shaking, filtering, and collecting filtrate to obtain test solution. The ultrasonic treatment power was 200W and the frequency was 50 kHz.
S3, content determination:
precisely sucking the reference solution and the sample solution, respectively, injecting into a liquid chromatograph, and measuring to obtain 10 active ingredient content measurement results shown in Table 2.
The detection conditions include: the chromatographic column was Kromasil C18(200 mm. times.4.6 mm, 5 μm); performing gradient elution by using acetonitrile-methanol aqueous solution with the volume ratio of 2: 1 as a mobile phase A and glacial acetic acid solution with the volume fraction of 0.05% as a mobile phase B, wherein the flow rate of the mobile phase is 1.0 mL/min; the column temperature was 25 ℃. The amount of sample was 10. mu.L.
During the gradient elution:
the volume fraction of the mobile phase A is changed from 10% to 25% in 0-25 min;
changing the volume fraction of the mobile phase A from 25% to 65% in 25-45 min;
and (4) changing the volume fraction of the mobile phase A from 65% to 85% in 45-60 min.
The detection wavelength is as follows: detection wavelength: 403nm in 0-12 min; 275nm in 12-18 min; 230nm for 18-45 min; 282nm in 45-60 min.
Table 2 results of content measurement of 10 active ingredients in the sample (mg/g, n ═ 3)
Experimental example:
first, examination of extraction conditions
1. Investigation of test solution preparation method:
experiments compare the extraction effects of different extraction methods such as water bath reflux, Soxhlet extraction, ultrasonic extraction and the like of different extraction solvents such as acetone, ethanol and methanol, and the result is that the overall effect of the ultrasonic extraction of the methanol is better. Meanwhile, the extraction effect of different concentrations of methanol (50%, 70% and 90%) as extraction solvents and different extraction times (5,10 and 20min) is also considered. The result shows that 70% methanol ultrasonic extraction is completed in 10min, the operation method is simple and convenient, and the method is stable and has good reproducibility. The methanol is adopted to extract a sample by ultrasonic, the obtained map information amount is large, fat-soluble components and water-soluble components are contained, all information contained in the medicinal materials is reflected as far as possible, and the method can be used as a comprehensive quality evaluation method.
2. Selection and optimization of chromatographic conditions:
in the selection of the mobile phase system, isocratic and gradient elution tests were performed on mobile phase systems with different volume fractions and different proportions, such as methanol-water, methanol-0.05% formic acid water, methanol-0.1% formic acid water, methanol-0.3% formic acid water, acetonitrile-0.05% glacial acetic acid water, acetonitrile-0.1% glacial acetic acid water, methanol-0.05% glacial acetic acid water, methanol-0.1% glacial acetic acid water, methanol-acetonitrile-0.05% glacial acetic acid water, methanol-acetonitrile-0.1% glacial acetic acid water, and the like. The result shows that the gradient elution is performed by using methanol-acetonitrile-0.05% glacial acetic acid water, the chromatographic peak detection is relatively comprehensive, the base line is stable, the peak shape is symmetrical, the separation degree is relatively good, the peak area is relatively large, after the elution proportion of the mobile phase at different time is adjusted, the retention time of each peak is moderate, the base line is relatively stable and is not easy to drift, the separation degree of the chromatogram is improved, the tailing phenomenon of the chromatogram is effectively avoided, and the quality analysis is facilitated.
3. Selection of detection wavelength:
performing spectral scanning on 11 components to be detected by adopting a photodiode array detector (PDAD) within the wavelength range of 190-450 nm to obtain the results that the maximum absorption wavelengths of strychnine, α -Chamaecyparis obtusa alkali, periplocin, 11-carbonyl- β -boswellic acid, zedoary bicyclenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone are respectively 258, 246, 220, 270, 210, 403, 283, 285, 282 and 248nm, and finally setting the wavelengths to be 403nm for 0-12 min, 275nm for 12-18 min, 230nm for 18-45 min and 282nm for 45-60 min by combining a liquid chromatogram.
4. Selection of analysis time:
the chromatogram for 1.5h was recorded when the elution time of the profile was chosen. The result shows that no obvious chromatographic peak appears after 55min, and in order to take care of the difference of the batch samples, the characteristic peaks of all the batch samples can be detected, so 60min is selected as the analysis time.
5. Selection of column temperature:
the influence of four different column temperatures (e.g. 25 ℃, 30 ℃, 35 ℃ and 40 ℃) on the fingerprint detection result is tested. The result shows that when the column temperature is 25 ℃, the retention time of chromatographic peaks is appropriate, the base line is stable, the separation degree of each chromatographic peak is good, and the peak shapes are symmetrical, so the column temperature is selected to be 25 ℃.
6. Selection of flow rate:
the effect of four flow rates (0.8ml/min,0.9ml/min,1.0ml/min,1.2ml/min) on the profile test results was tested. The results show that: when the flow rate is 1.0ml/min, the separation effect is optimal, the retention time of each chromatographic peak is proper, the separation degree is good, the base line is stable, and the peak shapes are symmetrical, so that the flow rate is 1.0 ml/min.
Second, system applicability test
Sucking 0.25mL of the mixed control solution in the example 1, and adding 70% methanol to dilute to 5 mL; the sample solution of example 1 was aspirated and filtered through a 0.22 μm microporous membrane. The 2 solutions are respectively injected under the chromatographic conditions of the example 1 for determination, and chromatograms are recorded. As a result, under the chromatographic condition, baseline separation can be achieved among components to be detected and among peaks of the components to be detected and impurities, the separation degree is larger than 1.5, other components do not interfere with the measurement of the components to be detected, and the number of theoretical plates is not lower than 5000 in terms of strychnine peaks.
Three, linear relationship investigation
Precisely sucking 0.25mL, 0.5 mL, 1 mL, 2mL, 4 mL and 5mL of the mixed reference substance solution in example 1, placing the mixed reference substance solution in different 5mL measuring bottles, diluting the mixed reference substance solution to a scale with 70% methanol to prepare 6 series reference substance solutions with different mass concentrations, performing sample injection measurement according to the chromatographic conditions in example 1, drawing a standard curve by taking the mass concentration of the reference substance as a horizontal coordinate (x) and taking a peak area as a vertical coordinate (y), and calculating a regression equation of each component. As a result, the linear relationship between the peak area and each component in each mass concentration range was good, and the results are shown in Table 3.
TABLE 3 results of linear relationship examination
| Composition (I) | Regression equation | Linear Range/. mu.g.mL-1 | r |
| Strychnine | Y＝30.924X-208.21 | 15.53～362.76 | 0.9997 |
| α A medicinal composition containing chamaecyparis obtusa alkali | Y＝158.47X+25.89 | 0.73～13.62 | 0.9998 |
| Periploca forrestii glycoside | Y＝68.216X-27.954 | 2.17～39.73 | 0.9997 |
| 11-carbonyl- β -boswellic acid | Y＝22.874X+17.258 | 2.67～53.69 | 0.9996 |
| Curcumae rhizoma bicycloheterone | Y＝55.792X+39.251 | 4.16～75.28 | 0.9998 |
| Hydroxy safflower yellow A | Y＝17.263X-19.261 | 3.69～81.44 | 0.9998 |
| Naringin | Y＝18.763X+51.625 | 15.93～361.58 | 0.9998 |
| Tetrandrine | Y＝103.54X-26.911 | 2.46～48.78 | 0.9996 |
| Fangchinoline | Y＝135.63X-41.792 | 1.59～31.75 | 0.9997 |
| β -ecdysterone | Y＝3.9672X+105.241 | 61.58～883.29 | 0.9996 |
Fourth, precision test
The appropriate amount of the mixed control solution in example 1 was precisely extracted, samples were continuously injected 6 times under the chromatographic conditions of example 1, and chromatograms were recorded, and RSDs of peak areas of strychnine, α -sabelline, periplocin, 11-carbonyl- β -boswellic acid, zedoary bicyclenone, hydroxysafflor yellow a, naringin, tetrandrine, fangchinoline, and β -ecdysterone were 0.56%, 0.39%, 1.04%, 0.68%, 0.75%, 0.92%, 0.73%, 0.97%, 0.81%, and 0.57% (n ═ 6), respectively, and RSDs of relative retention times were all less than 0.45% (n ═ 6), indicating that the instrument precision was good.
Fifth, repeatability test
The results of taking 6 samples of the same batch, preparing test solution according to the method of example 1, sampling and measuring under the chromatographic conditions of example 1, recording chromatograms, and recording the results, wherein the RSD of the peak areas of strychnine, α -Chamaecyparis obtusine, periplocin, 11-carbonyl- β -boswellic acid, zedoary dicyclenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone is less than 2.00% (n ═ 6), and the RSD of the relative retention time is less than 0.70% (n ═ 6), which indicates that the method has good repeatability.
VI stability test
The same sample solution was allowed to stand at room temperature for 0, 2, 4, 6, 8, 12, and 24 hours, and then subjected to sample injection measurement under the conditions of chromatography in example 1, and chromatogram was recorded, as a result, RSDs of peak areas of strychnine, α -sabelline, periplocin, 11-carbonyl- β -boswellic acid, curcumenone, hydroxysafflor yellow a, naringin, tetrandrine, fangchinoline, and β -ecdysterone were 1.52%, 2.37%, 1.85%, 1.73%, 2.07%, 1.98%, 2.35%, 3.18%, 2.67%, and 2.74% (n-7), respectively, and RSDs of relative retention times were all less than 0.84% (n-7), indicating that the sample was stable at room temperature for 24 hours.
Seventh, sample addition recovery test
Precisely sucking up 6 parts of standard solution with known content, respectively placing 15mL parts of standard solution into 50mL measuring bottles, respectively adding appropriate amount of reference substance of the component to be measured, preparing test solution according to the method of example 1, carrying out sample injection measurement under the chromatographic condition of example 1, recording peak area, and calculating the sample adding recovery rate and RSD value of each component. The results are shown in Table 4.
Table 4 sample recovery rate measurement results (n ═ 6)
As can be seen from Table 4, the average sample recovery rates of strychnine, α -chamaecyparis base, periplocin, 11-carbonyl- β -boswellic acid, zedoary dicyclo ketene, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone are 92.43% -99.81% and RSD is 1.55% -3.44% (n ═ 6), which indicates that the method has good accuracy.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (9)
1. The method for measuring the content of the multiple components of the Jingyaokang capsule is characterized by comprising the following steps:
s1, preparation of a mixed reference solution:
accurately weighing strychnine, α -chamaecyparis pisiferin, periplocin, 11-carbonyl- β -boswellic acid, zedoary dicyclo ketene, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone, respectively, dissolving in solvent to obtain mixed reference solution;
s2, preparation of a test solution:
extracting JINGYAOKANG Capsule with solvent, filtering, and collecting filtrate to obtain test solution;
s3, content determination:
precisely sucking the reference solution and the sample solution, respectively, injecting into a liquid chromatograph, and measuring.
2. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 1, wherein the solvent is methanol.
3. The method for measuring the contents of the multiple components in the Jingyaokang capsule according to claim 1, wherein in step S1, the control substances of strychnine, α -yubaine, periplocin, 11-carbonyl- β -boswellic acid, zedoary bicyclenone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and β -ecdysterone are precisely weighed, placed in a same 25mL measuring flask, diluted to scale with 70% methanol, and shaken to obtain the mixed control solution of the 10 components with the mass concentrations of 85.6 μ g/mL, 339.4 μ g/mL, 56.3 μ g/mL, 285.2 μ g/mL, 48.9 μ g/mL, 73.6 μ g/mL, 24.8 μ g/mL, 36.2 μ g/mL, 59.3 μ g/mL and 285.6 μ g/mL.
4. The method for measuring the contents of the multiple components in the Jingyaokang capsule according to claim 1, wherein in step S2, 0.5g of the contents of the Jingyaokang capsule is precisely weighed, 70% methanol is added to the capsule to achieve a constant volume of 50mL, the capsule is sealed and weighed, ultrasonic treatment is carried out for 10min, the capsule is weighed again, the mass loss is compensated by 70% methanol, the capsule is shaken up and filtered, and a subsequent filtrate is taken, so that a test solution is obtained.
5. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 4, wherein the ultrasonic treatment power is 200W, and the frequency is 50 kHz.
6. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 1, wherein in the gradient elution process:
the volume fraction of the mobile phase A is changed from 10% to 25% in 0-25 min;
changing the volume fraction of the mobile phase A from 25% to 65% in 25-45 min;
and (4) changing the volume fraction of the mobile phase A from 65% to 85% in 45-60 min.
7. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 1, wherein in step S3, the detection wavelengths are as follows: detection wavelength: 403nm in 0-12 min; 275nm in 12-18 min; 230nm for 18-45 min; 282nm in 45-60 min.
8. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 1, wherein in step S3, the detection conditions include: the chromatographic column is Kromasil C18; performing gradient elution by using acetonitrile-methanol aqueous solution with the volume ratio of 2: 1 as a mobile phase A and glacial acetic acid solution with the volume fraction of 0.05% as a mobile phase B, wherein the flow rate of the mobile phase is 1.0 mL/min; the column temperature was 25 ℃.
9. The method for measuring the contents of the components in the Jingyaokang capsule according to claim 1, wherein the sample size is 10 μ L in step S3.
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