CN113655165B - Fingerprint detection method of postpartum recovery ointment - Google Patents
Fingerprint detection method of postpartum recovery ointment Download PDFInfo
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Abstract
A fingerprint detection method of puerperal recovery ointment comprises the following steps: step 1, preparing a post-natal recovery paste test sample solution: putting a proper amount of postpartum recovery paste into a conical bottle with a plug, adding water, oscillating for dissolution, adding ethyl acetate, oscillating for extraction, standing, layering clearly up and down, taking an upper layer extract, extracting repeatedly for two times, fixing the volume of residues with methanol, centrifuging, filtering with an organic microporous filter membrane, and taking a subsequent filtrate to obtain a solution of a sample to be tested; step 2, preparing a postpartum recovery ointment reference substance solution; step 3, injecting the reference substance solution into a high performance liquid chromatograph, and recording a chromatogram; step 4, deriving the fingerprint, and importing the fingerprint into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system 2012A; the method can control the quality stability of the postpartum recovery ointment, ensure the safety and the effectiveness of the postpartum recovery ointment, and perfect the optimization of the related pharmaceutical process.
Description
Technical Field
The invention belongs to the technical field of detection of Chinese patent medicines, and particularly relates to a fingerprint detection method and a fingerprint of postpartum recovery ointment.
Background
The postpartum recovery ointment is a compound preparation composed of 20 medicinal materials such as astragalus, dangshen (stir-fried), angelica (stir-fried) and the like, has the effects of tonifying qi and nourishing blood, nourishing kidney and liver, soothing nerves and arresting vomiting, strengthening spleen and stomach and the like, and is used for postpartum anemia and abortion anemia, lochia, dizziness, palpitation and hyperhidrosis, insomnia and listlessness, inappetence and the like.
According to statistics, 2500 ten thousands of women in the national childbearing age become pregnant and give birth each year, and tens of thousands of women receive abortion operation, so that the health care and rehabilitation work of postpartum (including induced labor and abortion) is well done, and the health care and rehabilitation work has important significance for guaranteeing the physical health of women and children and improving the quality of human bodies.
The postpartum recovery ointment is taken as a pure traditional Chinese medicine preparation with the effects of tonifying qi and nourishing blood, soothing nerves and strengthening spleen, nourishing yin and tonifying liver, and can effectively treat postpartum anemia, lochia, dizziness and other gynecological postpartum symptoms.
Puerperal health contains various flavonoids, phenolic acids and alkaloids, and the existing content determination method cannot comprehensively and accurately evaluate the content of each effective component.
Therefore, the fingerprint detection method of the postpartum recovery ointment is established by adopting the high performance liquid chromatography on the basis of the prior art, and the method has important significance for component identification, quality evaluation and quality standard formulation of the postpartum recovery ointment.
Disclosure of Invention
The invention aims to: the invention aims to overcome the defects of the prior art, and develop a fingerprint detection method for postpartum recovery paste, which can control the quality stability of the postpartum recovery paste, ensure the safety and the effectiveness of the postpartum recovery paste and perfect the optimization of related pharmaceutical processes.
The technical scheme is as follows: in order to achieve the above purpose, the invention adopts the following technical scheme:
a fingerprint detection method of puerperal recovery ointment comprises the following steps:
placing a proper amount of puerperal recovery paste into a conical bottle with a plug, adding purified water, oscillating for dissolution, adding ethyl acetate, oscillating for extraction, standing, layering clearly, taking an upper layer extract, extracting repeatedly for two times, combining the extracts, evaporating in a water bath, fixing the volume of residues by methanol, centrifuging, taking a supernatant, filtering by using an organic microporous filter membrane with the size of 0.45 mu m, and taking a subsequent filtrate to obtain a solution of a sample to be tested;
respectively weighing appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substances, respectively placing into volumetric flasks, and adding methanol to prepare reference substance stock solutions respectively; then, respectively and precisely absorbing a proper amount of reference substance stock solution of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin, adding methanol to a certain volume, and preparing a mixed reference substance solution of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin;
As a preferable scheme, the fingerprint detection method of the postpartum recovery ointment is characterized in that,
the preparation method of the sample solution of the postpartum recovery ointment comprises the following steps:
placing 3ml of the postpartum recovery paste into a conical flask with a plug, adding 10ml of purified water, oscillating for dissolution, adding 20ml of ethyl acetate, oscillating for extraction, standing, layering clearly, taking an upper layer extract, extracting repeatedly for two times, combining the extracts, evaporating in a water bath to dryness, metering the volume of residues with 2ml of methanol, centrifuging, taking a supernatant, filtering with a 0.45 mu m organic microporous filter membrane, and taking a subsequent filtrate to obtain a solution of a sample to be tested;
as a preferable scheme, the fingerprint detection method of the postpartum recovery ointment is characterized in that,
respectively taking appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substances, precisely weighing, placing into a10 ml volumetric flask, and adding methanol to prepare reference substance stock solutions with the concentrations of 457 mug/ml, 591 mug/ml, 519 mug/ml, 456.5 mug/ml, 750 mug/ml, 423 mug/ml and 624 mug/ml respectively; and respectively precisely measuring appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substance stock solution, adding methanol to a volume of 20ml, and preparing a mixed reference substance solution containing 45.7 mug of vanillic acid, 59.1 mug of syringic acid, 51.9 mug of leonurine hydrochloride, 45.65 mug of hesperidin, 75 mug of glycyrrhizin, 42.3 mug of salvianolic acid B and 62.4 mug of quercetin per 1 ml.
As a preferred scheme, the fingerprint detection method of the postpartum recovery ointment comprises the following steps: octadecylsilane chemically bonded silica is used as a filler, formic acid water is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is carried out, the flow rate is 0.5-1.5 ml.min < -1 >, the column temperature is 25-40 ℃, and the detection wavelength is 200-360 nm;
as a preferred scheme, the fingerprint detection method of the postpartum recovery ointment is characterized in that the chromatographic conditions detected by the high performance liquid chromatograph in the step 3 are as follows: octadecylsilane chemically bonded silica is used as a filler, 0.1% formic acid water is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is carried out, the flow rate is 1.0 ml.min < -1 >, the column temperature is 30 ℃, and the detection wavelength is 254nm.
As a preferred scheme, the fingerprint detection method of the postpartum recovery ointment is characterized in that gradient elution is preferably as follows:
| time/min | Mobile phase a volume percent | Mobile phase B volume percentPercentage of |
| 0 | 95 | 5 |
| 15 | 95 | 5 |
| 55 | 90 | 10 |
| 80 | 85 | 15 |
| 160 | 70 | 30 |
| 170 | 65 | 35 |
| 175 | 95 | 5 |
| 180 | 95 | 5 |
。
As se:Sup>A preferred scheme, the fingerprint detection method of the postpartum recovery paste is characterized in that the chromatographic column is YMC-Pack ODS-A chromatographic column (250 mm multiplied by 4.6mm,5 μm).
As a preferred scheme, in the fingerprint detection method of the postpartum recovery ointment, 13 common peaks exist in the obtained fingerprint of the postpartum recovery ointment, and the retention time of each common peak is respectively as follows: peak 1 was shared with retention time 23.162min; peak 2 was shared with retention time 26.381min; peak 3 was shared with retention time 37.807min; peak 4 was shared with retention time 47.379min; peak 5 was shared with retention time 76.069min; peak 6 was shared with retention time 88.859min; peak 7 was shared with retention time 100.243min; peak 8 was shared with retention time 109.256min; peak 9 was shared with retention time 117.862min; peak 10 was shared with retention time 125.050min; peak 11 was shared with retention time 131.556min; peak 12 was shared with a retention time of 135.199min; peak 13 was shared and retention time was 168.875min.
Wherein, the No. 3 peak is vanillic acid, the No. 4 peak is syringic acid, the No. 5 peak is leonurine hydrochloride, the No. 6 peak is hesperidin, the No. 8 peak is glycyrrhizin, the No. 10 peak is salvianolic acid B, and the No. 11 peak is quercetin.
And (3) optimizing an experimental method and fingerprint detection conditions:
(1) Selection of different mobile phases:
according to the invention, a plurality of experiments are carried out to screen a mobile phase system such as pure water-acetonitrile, pure water-methanol, 0.1% formic acid water-acetonitrile, 0.1% formic acid water-methanol, 0.1% phosphoric acid water-acetonitrile, 0.1% phosphoric acid water-methanol and the like for detection, and the detection result shows that the mobile phase system contains 0.1% formic acid water and acetonitrile by volume, and the system has stable base line, peak shape and best condition of separation degree of each peak. Therefore, acetic acid containing 0.1% by volume of formic acid was selected as mobile phase A, and acetonitrile was selected as mobile phase B.
Because the effective components of the postpartum recovery ointment contain not only flavone and phenolic acid acidic components but also alkaloid components, the separation difficulty on the same chromatographic column is high. On the basis of determining a mobile phase, in order to achieve better separation degree, different gradient elution modes are screened, and experimental results show that the different gradient elution modes have great influence on the separation degree of each compound, and the optimal gradient elution modes are preferably obtained by adjusting the separation degree and the chromatographic peak number in different time periods: the ratio of mobile phase B varies as: 0-15 min,5% -5% acetonitrile; 15-55 min, 5-10% acetonitrile; 55-80 min, 10-15% acetonitrile, 80-160 min, 15-30% acetonitrile; 160-170 min, 30-35% acetonitrile; 170-175 min, 35-5% acetonitrile; 175-180 min,5% -5% acetonitrile;
(2) Selection of different detection wavelengths:
according to the invention, chromatographic detection results at different wavelengths of 230nm, 254nm, 280nm and 350nm are screened through a large number of experiments, and the detection results show that the intensity of each characteristic peak at 254nm is good, so that 254nm is selected as the detection wavelength, as shown in figure 1.
The beneficial effects of the invention are as follows:
(1) According to the structural property characteristics of active ingredients contained in the postpartum recovery ointment, the optimal mobile phase composition, the gradient elution program, the flow velocity, the detection wavelength, the chromatographic column, the column temperature and other analysis conditions are screened out through a large number of experiments, and the experimental verification shows that the ginseng and poria cocos wine fingerprint detection method provided by the invention can comprehensively, objectively and accurately detect and evaluate the quality of the postpartum recovery ointment and has important significance for ensuring the curative effect of the clinical postpartum recovery ointment.
(2) The fingerprint established by the invention can pay attention to the front-back sequence and the interrelation of the fingerprint characteristic peaks and pay attention to the overall face characteristics, thereby avoiding the unilateral performance of judging the quality of the postpartum recovery ointment by measuring individual chemical components and reducing the possibility of artificial treatment for reaching the quality standard.
(3) The method has the advantages of simplicity, convenience, stability, high precision and good repeatability.
Drawings
FIG. 1 is a chromatogram of a test sample of the postpartum recovery cream at 254nm.
FIG. 2 is a chromatogram of the control solution at 254nm.
Fig. 3 is a fingerprint spectrum of 10 batches of postpartum recovery cream test sample solution matching.
Detailed Description
Embodiments of the present invention will be described in detail with reference to examples, which are not to be construed as specific conditions, either as normal conditions or as recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
The apparatus used in the following examples
LC-20AT high performance liquid chromatograph (SPD-M20A diode array detector) (Shimadzu corporation), BP211D precision analytical balance (Beijing Sidoris instrument systems Co., ltd.), FA1004N precision electronic analytical balance (Beijing Sidoris instrument systems Co., ltd.), HH-6 digital display constant temperature water bath (Hengzhou Jiangnan laboratory, inc.), SHZ-D (III) circulating water vacuum pump (Xinshi instrument Co., ltd.), KQ5200DE type digital controlled ultrasonic cleaner (frequency: 40KHz, kunshan ultrasonic Co., ltd.).
The reagents used in the examples below were as follows: the vanillic acid analysis standard (lot number 110776-201503, purity 99.8%), the glycyrrhizin analysis standard (lot number 111610-201607, purity 93.1%), the salvianolic acid B analysis standard (lot number 111562-201917, purity 96.6%), the quercetin analysis standard (lot number 100081-201610, purity 99.1%), the leonurine hydrochloride analysis standard (lot number 111823-202005, purity 95.7%) were all purchased from the institute of food and drug assay, the syringic acid analysis standard (purity 98.0%) was purchased from Shanghai Shimadzude standard technical service, and the hesperidin analysis standard (lot number 161231, purity 98.0%) was purchased from the biological technology company of Nanson Bei Ga. Acetonitrile is chromatographic pure, water is ultrapure water, and formic acid is analytical pure. Postpartum recovery cream (Jiangsu He Sheng pharmaceutical Co., ltd., lot numbers 190301, 190401, 190402, 190501, 190502, 200101, 200102, 200301, 200401, 200601) other reagents (analytical purity).
Example 1
A fingerprint detection method of postpartum recovery ointment comprises the following steps:
(1) Preparation of test solution:
10 batches of post-partum health cream (Jiangsu sea pharmaceutical industry Co., ltd., batch numbers 190301, 190401, 190402, 190501, 190502, 200101, 200102, 200301, 200401, 200601) are taken, 3ml of post-partum health cream is placed in a conical flask with a plug, 10ml of purified water is added, shaking and dissolving are carried out, 20ml of ethyl acetate is added, shaking and extracting are carried out, standing is carried out, upper layer extract is taken after upper and lower layers are separated clearly, the extraction is repeated twice, the extract is combined, water bath is evaporated to dryness, residues are subjected to constant volume by 2ml of methanol, after centrifugation, supernatant is taken and filtered by an organic microporous filter membrane with the size of 0.45 mu m, and subsequent filtrate is taken, thus obtaining the post-partum health cream sample solution.
(2) Preparation of a control solution:
respectively taking appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substances, precisely weighing, placing into a10 ml volumetric flask, and adding methanol to prepare reference substance stock solutions with the concentrations of 457 mug/ml, 591 mug/ml, 519 mug/ml, 456.5 mug/ml, 750 mug/ml, 423 mug/ml and 624 mug/ml respectively; and respectively precisely measuring appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substance stock solution, adding methanol to a volume of 20ml, and preparing a mixed reference substance solution containing 45.7 mug of vanillic acid, 59.1 mug of syringic acid, 51.9 mug of leonurine hydrochloride, 45.65 mug of hesperidin, 75 mug of glycyrrhizin, 42.3 mug of salvianolic acid B and 62.4 mug of quercetin per 1 ml.
(3) Precisely sucking the 10 batches of post-production kang paste test sample solutions in the step 1 and the mixed reference substance solution in the step 2 respectively, injecting into a high performance liquid chromatograph, and recording chromatograms;
(4) The fingerprint of the 10 batches of postpartum recovery ointment test sample solutions obtained in the step 3 is led out and is led into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system 2012A; selecting chromatographic peaks existing in chromatograms of 10 batches of postpartum recovery paste as common peaks; the average value calculation method is used for generating a control fingerprint of the postpartum recovery ointment, as shown in figure 3, and the similarity evaluation result is shown in table 2. Calculating the relative retention time and the relative peak area of each common peak; and labeling chemical components of peaks in the control fingerprint according to a mixed control solution chromatogram (shown in fig. 2, peak 3 is vanillic acid, retention time is 37.803 min, peak 4 is syringic acid, retention time is 47.379min, peak 5 is leonurine hydrochloride, retention time is 76.069min, peak 6 is hesperidin, retention time is 88.859min, peak 8 is glycyrrhizin, retention time is 117.862min, peak 10 is salvianolic acid B, retention time is 131.554 min, peak 11 is quercetin, and retention time is 135.199 min).
Chromatographic conditions and System applicability
Chromatographic column: YMC-Pack ODS-A column (250 mm. Times.4.6 mm,5 μm).
Mobile phase: phase A: 0.1% by volume of formic acid water; and B phase: acetonitrile; detection wavelength: 254nm, flow rate: 1.0 ml/min -1 Column temperature: 30 ℃; gradient elution procedure is as follows table 1:
TABLE 1 gradient elution procedure
| Time/min | Mobile phase a volume percent | Mobile phase |
| 0 | 95 | 5 |
| 15 | 95 | 5 |
| 55 | 90 | 10 |
| 80 | 85 | 15 |
| 160 | 70 | 30 |
| 170 | 65 | 35 |
| 175 | 95 | 5 |
| 180 | 95 | 5 |
。
TABLE 2 evaluation results of similarity of solutions of samples of the post-partum Kanggao test
13 common peaks exist in the obtained postpartum recovery ointment fingerprint, and the retention time of each common peak is respectively as follows: peak 1 was shared with retention time 23.162min; peak 2 was shared with retention time 26.381min; peak 3 was shared with retention time 37.807min; peak 4 was shared with retention time 47.379min; peak 5 was shared with retention time 76.069min; peak 6 was shared with retention time 88.859min; peak 7 was shared with retention time 100.243min; peak 8 was shared with retention time 109.256min; peak 9 was shared with retention time 117.862min; peak 10 was shared with retention time 125.050min; peak 11 was shared with retention time 131.556min; peak 12 was shared with a retention time of 135.199min; peak 13 was shared and retention time was 168.875min.
Wherein, the No. 3 peak is vanillic acid, the No. 4 peak is syringic acid, the No. 5 peak is leonurine hydrochloride, the No. 6 peak is hesperidin, the No. 8 peak is glycyrrhizin, the No. 10 peak is salvianolic acid B, and the No. 11 peak is quercetin.
Example 2 methodology study of fingerprint detection:
1. methodology investigation
1.1 precision investigation
Taking a postpartum recovery paste sample with a lot number of 200601, preparing a test sample solution according to the test sample preparation method of the embodiment 1, continuously injecting sample for 6 times, detecting 10 mu L of sample injection amount each time according to the chromatographic conditions of the embodiment 1, measuring an HPLC chromatogram, and examining 10 common fingerprint peaks in the chromatogram, wherein the result shows that the retention time RSD of the common fingerprint peaks is less than 3.42 percent, the common peak area RSD is less than 1.89 percent, and the instrument precision is better.
1.2 stability investigation
Taking a postpartum recovery paste sample with a lot number of 200601, preparing a test sample solution according to the test sample preparation method of the embodiment 1, respectively carrying out sample injection at 0,4,8, 12, 16 and 24 hours according to the chromatographic conditions in the embodiment 1, recording a chromatogram, and examining 10 common fingerprint peaks in the chromatogram, wherein the result shows that the retention time RSD of the common fingerprint peaks is less than 1.22%, the common peak area RSD is less than 2.76%, and the test sample solution has good stability in 24 hours.
1.3 repeatability investigation
6 parts of postpartum recovery ointment sample with the batch number of 200601 are taken, sample solutions are prepared according to the sample preparation method of the example 1, the sample solutions are measured respectively, chromatograms are recorded, 6 common fingerprint peaks in the chromatograms are inspected, the result shows that the retention time RSD of the common fingerprint peaks is less than 0.59%, the common peak area RSD is less than 2.56%, and the method has good repeatability.
The experimental results show that the fingerprint detection method for the postpartum recovery ointment has good precision, stability and repeatability, can effectively represent the quality of the postpartum recovery ointment, and is beneficial to comprehensively monitoring the quality of the postpartum recovery ointment.
Claims (5)
1. The fingerprint detection method of the postpartum recovery ointment is characterized by comprising the following steps of:
step 1, preparing a sample solution of postpartum recovery paste:
putting a proper amount of postpartum recovery paste into a conical bottle with a plug, adding water, oscillating for dissolution, adding ethyl acetate, oscillating for extraction, standing, layering clearly up and down, taking an upper layer extract, extracting repeatedly for two times, combining the extracts, evaporating in a water bath, fixing the volume of residues by methanol, centrifuging, taking a supernatant, filtering by using an organic microporous filter membrane with the size of 0.45 mu m, and taking a subsequent filtrate to obtain a solution of a sample to be tested;
step 2, preparing a postpartum recovery ointment reference substance solution:
respectively weighing appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substances, respectively placing into volumetric flasks, and adding methanol to prepare reference substance stock solutions respectively; then, respectively and precisely absorbing a proper amount of reference substance stock solutions of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin, adding methanol to fix the volume, and preparing a mixed reference substance solution of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin;
step 3, respectively precisely sucking the postpartum recovery paste test sample solution in the step 1 and the mixed reference substance solution in the step 2, injecting into a high performance liquid chromatograph, and recording a chromatogram;
step 4, deriving the fingerprint of the postpartum recovery ointment test sample solution obtained in the step 3, and introducing the fingerprint into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system 2012A; selecting chromatographic peaks existing in chromatograms of different batches of postpartum recovery paste as common peaks; generating a control fingerprint of the postpartum recovery ointment by an average value calculation method, and calculating the relative retention time and the relative peak area of each common peak; labeling chemical components of peaks in the reference fingerprint according to the retention time of the mixed reference solution chromatogram;
the chromatographic conditions detected by the high performance liquid chromatograph in the step 3 are as follows: the chromatographic column is YMC-Pack ODS-A chromatographic column with the specification of 50mm multiplied by 4.6mm and 5 mu m, 0.1% formic acid water is used as se:Sup>A mobile phase A, acetonitrile is used as se:Sup>A mobile phase B, gradient elution is carried out, the flow rate is 1ml min < -1 >, the column temperature is 30 ℃, and the detection wavelength is 254nm;
gradient elution is as follows:
。
2. The fingerprint detection method of the postpartum recovery cream according to claim 1, which is characterized in that,
the preparation method of the sample solution of the postpartum recovery ointment comprises the following steps:
putting 3ml of the postpartum recovery paste into a conical flask with a plug, adding 10ml of purified water, oscillating for dissolution, adding 20ml of ethyl acetate, oscillating for extraction, standing, layering clearly, taking an upper layer extract, extracting repeatedly twice, combining the extracts, evaporating in a water bath to dryness, fixing the volume of residues with 2ml of methanol, centrifuging, taking supernatant, filtering with a 0.45 mu m organic microporous filter membrane, and taking subsequent filtrate to obtain a solution of a sample to be tested.
3. The fingerprint detection method of the postpartum recovery cream according to claim 1, which is characterized in that,
step 2, preparing a mixed reference substance solution:
respectively taking appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substances, precisely weighing, placing into a10 ml volumetric flask, and adding methanol to prepare reference substance stock solutions with the concentrations of 457 mug/ml, 591 mug/ml, 519 mug/ml, 456.5 mug/ml, 750 mug/ml, 423 mug/ml and 624 mug/ml respectively; and respectively precisely measuring appropriate amounts of vanillic acid, syringic acid, leonurine hydrochloride, hesperidin, glycyrrhizin, salvianolic acid B and quercetin reference substance stock solution, adding methanol to a volume of 20ml, and preparing a mixed reference substance solution containing 45.7 mug of vanillic acid, 59.1 mug of syringic acid, 51.9 mug of leonurine hydrochloride, 45.65 mug of hesperidin, 75 mug of glycyrrhizin, 42.3 mug of salvianolic acid B and 62.4 mug of quercetin per 1 ml.
4. The fingerprint detection method of the postpartum recovery cream according to claim 1, wherein 13 common peaks exist in the obtained fingerprint of the postpartum recovery cream, and the retention time of each common peak is respectively as follows: peak 1 was shared with retention time 23.162min; peak 2 was shared with retention time 26.381min; peak 3 was shared with retention time 37.807min; peak 4 was shared with retention time 47.379min; peak 5 was shared with retention time 76.069min; peak 6 was shared with retention time 88.859min; peak 7 was shared with retention time 100.243min; peak 8 was shared with retention time 109.256min; peak 9 was shared with retention time 117.862min; peak 10 was shared with retention time 125.050min; peak 11 was shared with retention time 131.556min; peak 12 was shared with a retention time of 135.199min; peak 13 was shared and retention time was 168.875min.
5. The method for detecting the fingerprint of the postpartum recovery cream according to claim 4, wherein the peak 3 is vanillic acid, the peak 4 is syringic acid, the peak 5 is leonurine hydrochloride, the peak 6 is hesperidin, the peak 8 is glycyrrhizin, the peak 10 is salvianolic acid B, and the peak 11 is quercetin.
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| CN103323563A (en) * | 2013-06-26 | 2013-09-25 | 江苏海昇药业有限公司 | Quality control method for postpartum rehabilitation ointment |
| CN109655565A (en) * | 2019-01-07 | 2019-04-19 | 南京海昌中药集团有限公司 | The fingerprint atlas detection method of yimucao paste |
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| CN103323563A (en) * | 2013-06-26 | 2013-09-25 | 江苏海昇药业有限公司 | Quality control method for postpartum rehabilitation ointment |
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