CN110974257A - Internal jugular vein indwelling blood sample collecting device and method for tree shrews - Google Patents
Internal jugular vein indwelling blood sample collecting device and method for tree shrews Download PDFInfo
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- CN110974257A CN110974257A CN201911401468.0A CN201911401468A CN110974257A CN 110974257 A CN110974257 A CN 110974257A CN 201911401468 A CN201911401468 A CN 201911401468A CN 110974257 A CN110974257 A CN 110974257A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/155—Devices specially adapted for continuous or multiple sampling, e.g. at predetermined intervals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/153—Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
- A61B5/154—Devices using pre-evacuated means
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
Abstract
The invention discloses a collecting device and a collecting method for an internal jugular vein indwelling blood sample of a tree shrew, and mainly relates to the technical field of tree shrew experimental blood collection. Comprises a puncture needle, an indwelling blood sampling hose and an indwelling box; the puncture needle comprises a detachable part and a needle handle; the detachable part comprises two detachable needle body parts and two detachable needle body fixing shells; the indwelling box comprises a seat body, a pipe body pressing and fixing rubber plug, a clamping seat, a sealing plug and a protective cap; the collection method is a using method step of the blood sample collection device. The invention has the beneficial effects that: the tree shrew blood sampling hose can be used for blood sampling for many times by one-time vascular puncture, is suitable for continuous blood sampling or blood sampling reserved in different time periods, has good fixing effect, is not easy to fall off, does not influence the remaining blood sampling hose reserved in the internal jugular vein by the movement of the neck of the tree shrew, and does not damage the wall of the venous blood vessel by the remaining blood sampling hose reserved in the internal jugular vein. Meanwhile, the indwelling blood sampling device can also be used for drug administration.
Description
Technical Field
The invention relates to the technical field of tree shrew experiment blood collection, in particular to a device and a method for collecting internal jugular vein indwelling blood samples of tree shrews.
Background
The tree shrew is a small-sized climbing mammal. The tree shrew whole genome sequencing analysis and a plurality of research results at home and abroad show that the genetic relationship of the tree shrew is closest to that of a primate (about 93.4 percent), is anatomically and physiologically closer to a human than common rodent experimental animals such as mice, rats and the like, can suffer from a plurality of diseases similar to the human, has unique application advantages in the research of animal models of human diseases, and is mainly used for the research in the fields of tumors, nerves, incretion, immunity, viruses, bacteria, parasites and the like. The tree shrew is often required to be subjected to blood sampling and drug administration for multiple times in the research. Because internal jugular vein blood sampling can obtain a larger amount of blood samples than heart blood sampling, retroorbital venous plexus blood sampling and other modes, the internal jugular vein blood sampling becomes the main blood sampling mode in the tree shrew experiment at present, and certainly, because the lower limbs are easy to press, hold and fix, the femoral vein at the groin of the lower limbs is also more generally used for puncture blood sampling.
The current internal jugular vein blood sampling method generally comprises the following steps: the tree shrew is fixed on the experiment table in a supine position, the head and the lower limbs of the tree shrew are fixed by hands to assist an experimenter to see the convex blood vessels after the blood vessels are full, the needle is inserted by an injector at an included angle of 45 degrees, and the blood is sucked out, wherein the blood taking amount is less than 2ml each time. In practice, a 1ml or 2.5ml syringe is usually used to take the internal jugular vein blood sample. However, there are many disadvantages and inconveniences in this method.
In the experimental process, the tree shrew is generally required to be subjected to blood sampling or drug administration for multiple times within a certain time, for example, the position of the internal jugular vein shown in the attached drawing of the specification and fig. 6 is that the internal jugular vein of the tree shrew is only about 3mm, the internal jugular vein protrudes unobviously during actual operation, the puncture has certain failure risk, meanwhile, the neck of the tree shrew can move in a stretching mode during operation, the blood vessel is easy to be punctured by a needle, the excessive damage to the blood vessel of the tree shrew is caused, the blood infection risk is increased by multiple punctures within a short time, and the experimental result is influenced. For operators, the tree shrew blood vessel needs to be punctured every time of blood sampling, the operation is more complicated, and valuable scientific research time is wasted.
Disclosure of Invention
The invention aims to provide a collecting device and a collecting method for an internal jugular vein indwelling blood sample of a tree shrew, which are simple, convenient and efficient to use, can be used for collecting blood for multiple times by one-time vascular puncture, are suitable for continuous blood collection or indwelling blood collection in different time periods, have good fixing effect, are not easy to fall off, cannot influence an indwelling blood collecting hose indwelling in the internal jugular vein by the movement of the neck of the tree shrew, and cannot damage the wall of the venous blood vessel by the indwelling blood collecting hose indwelling in the internal jugular vein. Meanwhile, the indwelling blood sampling device can also be used for drug administration. Furthermore, the device is not only suitable for tree shrews, but also suitable for all small experimental animals.
In order to achieve the purpose, the invention is realized by the following technical scheme:
1. a internal jugular vein indwelling blood collection system for tree shrew, its characterized in that: comprises a puncture needle, an indwelling blood sampling hose and an indwelling box;
puncture needle:
the puncture needle comprises a detachable part and a needle handle; the detachable part comprises two detachable needle body parts and two detachable needle body fixing shells, the tail ends of the two needle body parts are respectively connected with the two needle body fixing shells, the two needle body parts and the two needle body fixing shells are symmetrically folded, a branch pipe is arranged on the side part of the needle body fixing shell, the head end of the indwelling blood sampling hose is inserted into a needle cavity enclosed by the two needle body parts through the pipe orifice of the branch pipe, the indwelling box is arranged at the tail end of the indwelling blood sampling hose, and the tail end of the folded needle body fixing shell is detachably connected to the needle handle;
a retention box:
the indwelling box comprises a seat body, a pipe body pressing and fixing rubber plug, a clamping seat, a sealing plug and a protective cap; the top of the seat body is provided with a convex seat, the top of the convex seat is provided with a glue plug hole, a hose passage extending to the glue plug hole is arranged in the seat body, an inner side port of the indwelling blood sampling hose is indwelling in an internal jugular vein along the blood flow direction, and an outer side port of the indwelling blood sampling hose extends into the glue plug hole; the middle part of the tube body pressing and fixing rubber plug is provided with a central hole, the outer side port of the indwelling blood sampling hose is inserted into the central hole, and the tube body pressing and fixing rubber plug is inserted into the rubber plug hole; the clamping seat comprises a seat shell, a central insertion pipe and a central cushion groove; the seat shell is clamped with the convex seat, and after the seat shell is clamped with the convex seat, the central cannula is inserted into the outer side port of the indwelling blood sampling hose and can support the outer side port of the indwelling blood sampling hose tightly; the sealing plug is arranged in the central pad groove; the protective cap is detachably covered above the sealing plug.
The detachable part also comprises an elastic hoop sleeve, after the two needle body parts and the two needle body fixing shells are symmetrically folded, the elastic hoop sleeve is sleeved on the folded needle body parts, so that no gap exists at the folding part of the two needle body parts.
The needle handle is provided with a shell connector, and the tail end of the folded needle body fixing shell can be detachably clamped and fixed on the shell connector.
The center of the upper surface of the sealing plug is provided with a bulge.
5. A collection method of an internal jugular vein indwelling blood sample collection device for a tree shrew comprises the following steps:
s1, assembling the detachable part and the needle handle of the puncture needle to symmetrically fold the two needle body parts and the two needle body fixing shells, sleeving the folded needle body parts with the elastic hoop after folding, detachably clamping and fixing the tail ends of the folded needle body fixing shells on a shell connector, inserting the head end of an indwelling blood sampling hose into a needle cavity enclosed by the two needle body parts through a pipe orifice of a branch pipe, and connecting the tail end of the indwelling blood sampling hose with an indwelling box;
s2, using an animal shaver to remove the body hair at the position of the internal jugular vein of the tree shrew, and exposing the body surface at the position of the neck;
s3, fixing the tree shrew on a test bench in a supine position, and assisting an experimenter to fix the head and the lower limbs of the tree shrew by hands;
s4, cleaning and disinfecting the exposed part of the neck by using alcohol cotton;
s5, aligning the needle body to the internal jugular vein, inserting the needle towards the blood flow direction with an included angle of 45 degrees, and inserting the tail end of the indwelling blood collection hose in the needle body after the puncture needle enters the internal jugular vein into the internal jugular vein;
s6, pushing a remaining blood collection hose connected with a remaining box outside the branch pipe, wherein one end of the remaining blood collection hose located in the internal jugular vein extends out of the needle head end of the needle body part into the internal jugular vein;
s7, pulling out the puncture needle from the internal jugular vein after the indwelling blood collection hose is pushed for a certain distance; after the puncture needle is pulled out of the internal jugular vein, a part of pipe section of the indwelling blood sampling hose is indwelling in the internal jugular vein;
s8, after the puncture needle is pulled out of the internal jugular vein, the elastic hoop sleeve is cut off by the blade, then the detachable part is detached from the shell joint on the needle handle, the two detachable needle body parts and the two detachable needle body fixing shells are detached, the indwelling blood sampling hose in the detachable part is taken out, and at the moment, only one section of indwelling blood sampling hose and the indwelling box are exposed;
s9, because the length of the exposed section of the indwelling blood sampling hose is longer, the section of the indwelling blood sampling hose is coiled for a circle and is attached to the skin surface layer of the neck, and after the bottom of the indwelling box is also attached to the skin surface layer, the section of the indwelling blood sampling hose is stably attached and fixed to the skin surface layer of the neck by using a medical adhesive tape;
s10, when blood is collected, the protective cap on the retention box is taken down, then the upper surface of the sealing plug is disinfected by alcohol cotton, and the blood collection needle on the negative pressure blood collection test tube is aligned with the bulge to pierce the sealing plug for blood collection;
s11, after blood collection is finished, the upper surface of the sealing plug is disinfected by alcohol cotton, and then a protective cap is covered to prevent the upper surface of the sealing plug from being polluted by bacteria, fungi, viruses and other microorganisms in the outside air; waiting for the next blood sampling;
s12, repeating the steps S10 and S11 when blood is collected next time.
Compared with the prior art, the invention has the beneficial effects that:
by using the device according to the using method, one-time blood vessel puncture and multiple blood sampling can be really realized. Is suitable for continuous blood sampling or indwelling blood sampling at different time intervals. The tree shrew blood sampling hose is simple and convenient in design and efficient to use, the fixing effect of the design is good, the tree shrew neck is not easy to fall off, the remaining blood sampling hose remaining in the internal jugular vein cannot be influenced by the movement of the tree shrew neck, and the remaining blood sampling hose remaining in the internal jugular vein cannot damage the wall of the venous blood vessel. Meanwhile, the indwelling blood sampling device can also be used for drug administration. The device is not only suitable for the tree shrews, but also suitable for all small experimental animals.
Drawings
FIG. 1 is a state diagram of the present invention in use.
FIG. 2 is an enlarged view of portion I of FIG. 1 according to the present invention.
FIG. 3 is a schematic structural view of the indwelling box of the present invention.
Figure 4 is an exploded view of the retention box of the present invention.
Figure 5 is a schematic view of the structure of the puncture needle of the present invention.
Fig. 6 is an illustration of the internal jugular vein position. .
FIG. 7 is a schematic view showing the structure of the puncture device according to the present invention.
Fig. 8 is a femoral vein diameter measurement view.
Fig. 9 is a drawing showing a blood sampling operation by femoral vein puncture.
Reference numerals shown in the drawings:
1. puncturing needle; 2. keeping the blood sampling hose; 3. a retention box; 4. a detachable portion; 5. a needle handle; 6. a needle body portion; 7. a needle body fixing shell; 8. a branch pipe; 9. a base body; 10. the tube body presses and fixes the rubber plug; 11. a card holder; 12. a sealing plug; 13. a protective cap; 14. a boss; 15. plugging holes with glue; 16. a hose passage; 17. a central bore; 18. a seat shell; 19. a central cannula; 20. a central pad slot; 21. an elastic cuff; 22. a housing joint; 23. a protrusion; 24. a medical adhesive tape.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.
Because the tree shrew is easy to press and fix the lower limbs, the femoral vein at the groin of the lower limbs is more commonly used for puncture blood sampling. As the diameter measurement chart of the femoral vein shown in figure 8 of the attached drawings of the specification, the diameter of the femoral vein is only 1mm, and is far smaller than the internal jugular vein with the diameter of 3 mm. Therefore, as shown in the attached figure 9 of the specification, when the femoral vein is used for blood sampling, only a 0.45mm needle can be used for puncture blood sampling, so that the blood sampling speed is slow, and because the diameter of the femoral vein is only 1mm, if the tree shrew lower limb moves in the blood sampling process, the blood vessel is easy to be punctured by the needle, so that the tree shrew blood vessel is excessively damaged. And the blood vessel is punctured by the needle head and then tissue fluid is pumped to cause blood pollution, and the blood sampling can not be used any more. In addition, because the tree shrew has large four-limb movement amount and small diameter of femoral vein, the placement of an indwelling blood sampling device at the femoral vein is impractical, and the falling off or the injury of the blood vessel wall is easily caused.
Because of the large diameter of the internal jugular vein, the internal jugular vein blood sampling can obtain a larger amount of blood samples compared with other modes, and the blood sampling speed is higher. However, the internal jugular vein blood sampling method needs to fix the tree shrew on the experiment table in a supine position, and assists experimenters to fix the head and the lower limbs of the tree shrew by hands, and the only defect is that the supine tree shrew cannot be pressed and fixed conveniently, but the defect is only that blood is sampled by multiple times of puncture. Because the neck of the tree shrew is exposed, no other limbs are arranged around the tree shrew, and the neck movement range is small, if the internal jugular vein can be applied for design, a device capable of keeping blood collection is designed, and the internal jugular vein is an excellent application part. Although the supine tree shrew is pressed and fixed with certain difficulty during puncture, one-time vascular puncture can be used for blood sampling for a plurality of times for indwelling blood sampling.
The following contents are a technical principle for indwelling blood sampling at the internal jugular vein, the design is simple and convenient, the use is efficient, and one-time blood vessel puncture and multiple blood sampling are realized. Is suitable for continuous blood sampling or indwelling blood sampling at different time intervals. And the fixing effect of the design is good, the tree shrew is not easy to fall off, the movement of the neck of the tree shrew cannot influence the indwelling blood sampling hose indwelling in the internal jugular vein, and the indwelling blood sampling hose indwelling in the internal jugular vein cannot damage the vein wall. Meanwhile, the indwelling blood sampling device can also be used for drug administration. The device is not only suitable for the tree shrews, but also suitable for all small experimental animals.
The indwelling blood sampling device has the following technical structure, principle and using method:
the invention relates to a device and a method for collecting internal jugular vein indwelling blood samples for tree shrews, wherein the main structure of the device for collecting the internal jugular vein indwelling blood samples is as follows:
1. a puncture needle 1:
as shown in figure 5 of the accompanying drawings. The puncture needle 1 structurally comprises: a detachable portion 4 and a needle handle 5; the detachable part 4 comprises two detachable needle body parts 6, two detachable needle body fixing shells 7 and an elastic hoop sleeve 21, and the tail ends of the two needle body parts 6 are respectively connected with the two needle body fixing shells 7. After the two needle body parts 6 and the two needle body fixing shells 7 are symmetrically folded, the elastic hoop sleeve 21 is sleeved on the folded needle body parts 6, so that no gap exists at the folding position of the two needle body parts 6. The lateral part of the needle body fixing shell 7 is provided with a branch pipe 8, the head end of the indwelling blood sampling hose 2 is inserted into a needle cavity enclosed by the two needle body parts 6 through the pipe orifice of the branch pipe 8, and the tail end of the indwelling blood sampling hose 2 is provided with an indwelling box 3. The needle handle 5 is provided with a shell joint 22, and the tail end of the folded needle body fixing shell 7 is detachably clamped and fixed on the shell joint 22.
2. The indwelling box 3:
as shown in figures 2, 3 and 4 of the drawings attached to the specification. The whole indwelling box 3 is a box body assembly consisting of a plurality of parts, has the functions of being used for keeping the stability of the indwelling blood sampling hose 2 to treat blood sampling and drug administration, has good fixing effect and is not easy to fall off, the movement of the neck of the tree shrew cannot influence the indwelling blood sampling hose 2 kept in the internal jugular vein, and the indwelling blood sampling hose 2 kept in the internal jugular vein cannot damage the vein wall.
The structure of indwelling case 3 includes: the base body 9, the pipe body press-hold fixed rubber plug 10, the clamping base 11, the sealing plug 12 and the protective cap 13;
a seat body 9: the top of the seat body 9 is provided with a convex seat 14, the top of the convex seat 14 is provided with a glue plug hole 15, the seat body 9 is internally provided with a hose channel 16, and the hose channel 16 extends to the glue plug hole 15. The port at the inner side of the indwelling blood sampling hose 2 is indwelling in the internal jugular vein along the blood flow direction, and the port at the outer side of the indwelling blood sampling hose 2 extends into the glue plug hole 15.
The tube body presses and fixes the rubber plug 10: the middle part of the tube body is pressed and held with the fixed rubber plug 10 and is provided with a center hole 17, the outer side port of the indwelling blood sampling hose 2 is inserted into the center hole 17, the tube body is pressed and held with the fixed rubber plug 10 and is inserted into the rubber plug hole 15, the tube body is pressed and held with the fixed rubber plug 10 and is extruded by the inner wall of the rubber plug hole 15 to reduce the aperture of the center hole 17, thereby the reduced center hole 17 slightly deforms to the indwelling blood sampling hose 2, the pipe diameter of the indwelling blood sampling hose 2 is slightly pressed and held and is reduced, the friction between the wall of the center hole 17 and the indwelling blood sampling hose 2 is increased, and the indwelling blood sampling.
The clamping seat 11: the clamping seat 11 comprises a seat shell 18, a central insertion pipe 19 and a central cushion groove 20; seat shell 18 and boss 14 can the joint, and behind seat shell 18 and the boss 14 joint, central intubate 19 is inserted to keeping somewhere in blood sampling hose 2 outside port to can prop tightly keep somewhere blood sampling hose 2 outside port, so, keep somewhere blood sampling hose 2 outside port inside and outside receive central intubate 19 and the two-way pressure of central hole 17 wall simultaneously and hold the extrusion, thereby further effectively avoided keeping somewhere blood sampling hose 2 from central hole 17 landing.
Sealing plug 12: the sealing plug 12 is arranged in the central pad groove 20, the sealing plug 12 can realize the sealing of the top pipe opening of the central insertion pipe 19, the upper surface of the sealing plug 12 is a concave arc surface which is convenient for the disinfection of alcohol cotton before blood sampling, a bulge 23 is arranged at the central position of the upper surface of the sealing plug 12, and a blood sampling needle is aligned to the bulge 23 to pierce the sealing plug 12 for blood sampling during the blood sampling.
Protective cap 13: after the blood collection is completed, the upper surface of the sealing plug 12 is disinfected by alcohol cotton, and then the protective cap 13 is covered, so that the upper surface of the sealing plug 12 is prevented from being polluted by bacteria, fungi, viruses and other microorganisms in the outside air.
The using method of the internal jugular vein indwelling blood sample collecting device is as follows:
preparation before puncture: comprises steps S1, S2, S3 and S4;
s1, first, the detachable portion 4 and the needle shaft 5 of the puncture needle 1 shown in FIG. 5 of the specification are assembled. The two needle body parts 6 and the two needle body fixing shells 7 are symmetrically folded, and the folded needle body parts 6 are sleeved with the elastic hoop sleeves 21, so that no gap exists at the folding position of the two needle body parts 6. The closed needle body fixing shell 7 is detachably clamped and fixed on the shell connector 22 at the tail end. The head end of the indwelling blood collection hose 2 is inserted into a needle cavity enclosed by the two needle body parts 6 through the pipe orifice of the branch pipe 8, and the tail end of the indwelling blood collection hose 2 is connected with the indwelling box 3. Selecting a model: the needle body part 6 adopts a 16G type needle (the outer diameter of the needle is 1.61mm, the inner diameter of the needle is 1.25mm, and the wall thickness is 0.18mm), and the indwelling blood collection hose 2 adopts a hose with the outer diameter of 1.15mm, the inner diameter of 0.9mm and the wall thickness of 0.125 mm.
S2, next, the tree shrew was debrided of the body hair at the location of the internal jugular vein using an animal shaver, leaving the body surface exposed at the neck. Therefore, the puncture needle is convenient for searching and puncturing the internal jugular vein, and is also beneficial to fixing the indwelling box 3 after puncturing by adopting the medical adhesive tape 24.
And S3, fixing the tree shrew on a laboratory bench in a supine position, and assisting an experimenter to fix the head and the lower limbs of the tree shrew by hands.
And S4, cleaning and disinfecting the exposed part of the neck by using alcohol cotton.
The steps during puncture are as follows: comprising steps S5, S6;
s5, as shown in the figure 6 of the attached drawings, the needle handle 5 of the puncture needle 1 is held by hand, the needle body 6 is aligned with the internal jugular vein, and the needle is inserted with an included angle of 45 degrees towards the blood flow direction (namely the head → the trunk direction). The puncture needle 1 enters into the internal jugular vein and the end of the indwelling blood sampling hose 2 in the needle body part 6 also can be inserted into the internal jugular vein, then the branch pipe 8 is pushed again and the outside is connected with the indwelling blood sampling hose 2 of the indwelling box 3, and the end of the indwelling blood sampling hose 2 located in the internal jugular vein can stretch out to the internal jugular vein from the end of the needle head of the needle body part 6. After the pushing distance of the indwelling blood collection hose 2 is about 1.5cm, the puncture needle 1 is pulled out from the internal jugular vein, and because the tail end of the indwelling blood collection hose 2 extends out to about 1.5cm in the internal jugular vein from the needle head end of the needle body part 6, after the puncture needle 1 is pulled out from the internal jugular vein, part of the indwelling blood collection hose 2 with the length of 1.5cm can be remained in the internal jugular vein.
S6, after the puncture needle 1 is pulled out from the internal jugular vein, the elastic hoop 21 is cut off by the blade, then the detachable part 4 and the shell connector 22 on the needle handle 5 are detached, then the two detachable needle body parts 6 and the two detachable needle body fixing shells 7 are detached, and the indwelling blood collection hose 2 in the detachable part 4 is taken out. At this time, only one section of the indwelling blood collection hose 2 and the indwelling case 3 is exposed.
A post-puncture step: includes step S7;
s7, because the length of the exposed section of the indwelling blood collection hose 2 is longer, the section of the indwelling blood collection hose 2 is coiled for a circle and is attached to the surface layer of the neck skin, and after the bottom of the indwelling box 3 is also attached to the surface layer of the skin, the section of the indwelling blood collection hose 2 and the indwelling box 3 are stably attached and fixed to the surface layer of the neck skin by the medical adhesive tape 24.
A blood collection step: comprising steps S8, S9, S10;
s8, when blood sampling is carried out, the protective cap 13 on the retention box 3 is taken down, then the upper surface of the sealing plug 12 is disinfected by alcohol cotton, and the blood sampling needle on the negative pressure blood sampling test tube is aligned with the bulge 23 to pierce the sealing plug 12 for blood sampling.
S9, after blood collection is completed, the upper surface of the sealing plug 12 is disinfected by alcohol cotton, and then the protective cap 13 is covered to prevent the upper surface of the sealing plug 12 from being polluted by bacteria, fungi, viruses and other microorganisms in the outside air. Waiting for the next blood sampling.
S10, repeating the steps S8 and S9 when blood is collected next time.
In summary, the following steps:
by using the device according to the using method, one-time blood vessel puncture and multiple blood sampling can be really realized. Is suitable for continuous blood sampling or indwelling blood sampling at different time intervals. The technology for indwelling blood sampling at the internal jugular vein has the advantages of simple and convenient design, high use efficiency, good fixing effect and difficult falling-off, the movement of the neck of the tree shrew cannot influence the indwelling blood sampling hose 2 indwelling in the internal jugular vein, and the indwelling blood sampling hose 2 indwelling in the internal jugular vein cannot damage the wall of the venous blood vessel. Meanwhile, the indwelling blood sampling device can also be used for drug administration. The device is not only suitable for the tree shrews, but also suitable for all small experimental animals.
Claims (5)
1. A internal jugular vein indwelling blood collection system for tree shrew, its characterized in that: comprises a puncture needle (1), an indwelling blood sampling hose (2) and an indwelling box (3);
puncture needle (1):
the puncture needle (1) comprises a detachable part (4) and a needle handle (5); the detachable part (4) comprises two detachable needle body parts (6) and two detachable needle body fixing shells (7), the tail ends of the two needle body parts (6) are respectively connected with the two needle body fixing shells (7), the two needle body parts (6) and the two needle body fixing shells (7) are symmetrically folded, a branch pipe (8) is arranged on the side part of the needle body fixing shell (7), the head end of the indwelling blood sampling hose (2) is inserted into a needle cavity enclosed by the two needle body parts (6) through a pipe opening of the branch pipe (8), the indwelling box (3) is arranged at the tail end of the indwelling blood sampling hose (2), and the tail end of the folded needle body fixing shell (7) is detachably connected to the needle handle (5);
indwelling case (3):
the indwelling box (3) comprises a seat body (9), a pipe body pressing and fixing rubber plug (10), a clamping seat (11), a sealing plug (12) and a protective cap (13);
a convex seat (14) is arranged at the top of the seat body (9), a glue plug hole (15) is arranged at the top of the convex seat (14), a hose passage (16) extending to the glue plug hole (15) is arranged in the seat body (9), an inner side port of the indwelling blood sampling hose (2) is indwelling in an internal jugular vein along the blood flow direction, and an outer side port of the indwelling blood sampling hose (2) extends into the glue plug hole (15);
the middle part of the tube body pressing and fixing rubber plug (10) is provided with a central hole (17), the outer side port of the indwelling blood sampling hose (2) is inserted into the central hole (17), and the tube body pressing and fixing rubber plug (10) is inserted into the rubber plug hole (15);
the clamping seat (11) comprises a seat shell (18), a central insertion pipe (19) and a central cushion groove (20); the seat shell (18) is clamped with the convex seat (14), and after the seat shell (18) is clamped with the convex seat (14), the central insertion tube (19) is inserted into an outer side port of the indwelling blood sampling hose (2) and can tightly support the outer side port of the indwelling blood sampling hose (2);
the sealing plug (12) is arranged in the central pad groove (20);
the protective cap (13) can be detachably covered above the sealing plug (12).
2. The internal jugular vein indwelling blood sample collection device for a tree shrew according to claim 1, wherein: the detachable part (4) further comprises an elastic hoop sleeve (21), after the two needle body parts (6) and the two needle body fixing shells (7) are symmetrically folded, the elastic hoop sleeve (21) is sleeved on the folded needle body parts (6), and therefore no gap exists at the folding position of the two needle body parts (6).
3. The internal jugular vein indwelling blood sample collection device for a tree shrew according to claim 1, wherein: the needle handle (5) is provided with a shell connector (22), and the tail end of the folded needle body fixing shell (7) can be detachably clamped and fixed on the shell connector (22).
4. The internal jugular vein indwelling blood sample collection device for a tree shrew according to claim 1, wherein: and a bulge (23) is arranged at the center of the upper surface of the sealing plug (12).
5. A collection method for the internal jugular vein indwelling blood sample collection device for tree shrew according to any one of claims 1 to 4, comprising: the method comprises the following steps:
s1, assembling the detachable part (4) and the needle handle (5) of the puncture needle (1), symmetrically folding the two needle body parts (6) and the two needle body fixing shells (7), sleeving the folded needle body parts (6) with the elastic hoop sleeves (21), detachably clamping and fixing the tail ends of the folded needle body fixing shells (7) on a shell connector (22), inserting the head end of the remaining blood collection hose (2) into a needle cavity enclosed by the two needle body parts (6) through a pipe opening of a branch pipe (8), and connecting the tail end of the remaining blood collection hose (2) with the remaining box (3);
s2, using an animal shaver to remove the body hair at the position of the internal jugular vein of the tree shrew, and exposing the body surface at the position of the neck;
s3, fixing the tree shrew on a test bench in a supine position, and assisting an experimenter to fix the head and the lower limbs of the tree shrew by hands;
s4, cleaning and disinfecting the exposed part of the neck by using alcohol cotton;
s5, aligning the needle body (6) to the internal jugular vein, inserting the needle towards the blood flow direction by adopting an included angle of 45 degrees, and inserting the tail end of the indwelling blood sampling hose (2) in the needle body (6) into the internal jugular vein after the puncture needle (1) enters the internal jugular vein;
s6, then pushing the indwelling blood collection hose (2) of the branch pipe (8) to which the indwelling box (3) is connected, wherein one end of the indwelling blood collection hose (2) located in the internal jugular vein extends out of the needle head end of the needle body part (6) into the internal jugular vein;
s7, pulling out the puncture needle (1) from the internal jugular vein after the indwelling blood collection hose (2) is pushed for a certain distance; after the puncture needle (1) is pulled out of the internal jugular vein, a part of pipe section of the indwelling blood sampling hose (2) is indwelling in the internal jugular vein;
s8, after the puncture needle (1) is pulled out of the internal jugular vein, the elastic hoop sleeve (21) is cut off by a blade, then the detachable part (4) and the shell joint (22) on the needle handle (5) are detached, then the two detachable needle body parts (6) and the two detachable needle body fixing shells (7) are detached, the indwelling blood collection hose (2) in the detachable part (4) is taken out, and only one section of the indwelling blood collection hose (2) and the indwelling box (3) are exposed at this time;
s9, because the length of the exposed section of the indwelling blood sampling hose (2) is longer, the section of the indwelling blood sampling hose (2) is coiled for a circle and is adhered to the surface layer of the skin of the neck, and after the bottom of the indwelling box (3) is also adhered to the surface layer of the skin, the section of the indwelling blood sampling hose (2) and the indwelling box (3) are stably adhered and fixed to the surface layer of the skin of the neck by using the medical adhesive tape (24);
s10, when blood sampling is carried out, the protective cap (13) on the retention box (3) is taken down, then the upper surface of the sealing plug (12) is disinfected by alcohol cotton, and the blood sampling needle on the negative pressure blood sampling test tube pierces the sealing plug (12) to carry out blood sampling aiming at the bulge (23);
s11, after blood collection is finished, disinfecting the upper surface of the sealing plug (12) by alcohol cotton, and then covering a protective cap (13) to prevent the upper surface of the sealing plug (12) from being polluted by bacteria, fungi, viruses and other microorganisms in the outside air; waiting for the next blood sampling;
s12, repeating the steps S10 and S11 when blood is collected next time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911401468.0A CN110974257A (en) | 2019-12-31 | 2019-12-31 | Internal jugular vein indwelling blood sample collecting device and method for tree shrews |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911401468.0A CN110974257A (en) | 2019-12-31 | 2019-12-31 | Internal jugular vein indwelling blood sample collecting device and method for tree shrews |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113180658A (en) * | 2021-04-26 | 2021-07-30 | 山西医科大学 | Large animal percutaneous puncture femoral artery catheterization blood sampling device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113180658A (en) * | 2021-04-26 | 2021-07-30 | 山西医科大学 | Large animal percutaneous puncture femoral artery catheterization blood sampling device |
| CN113180658B (en) * | 2021-04-26 | 2022-07-01 | 山西医科大学 | Large animal percutaneous puncture femoral artery catheterization blood sampling device |
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