CN110967283A

CN110967283A - Automatic semen leucocyte detection device

Info

Publication number: CN110967283A
Application number: CN201911194994.4A
Authority: CN
Inventors: 刘海儒; 樊兴文; 张海琼; 刘春明; 蓝幸; 唐莹
Original assignee: Liuzhou Maternity and Child Healthcare Hospital
Current assignee: Liuzhou Maternity and Child Healthcare Hospital
Priority date: 2019-11-28
Filing date: 2019-11-28
Publication date: 2020-04-07

Abstract

The invention relates to the technical field of medical instruments, in particular to an automatic semen leucocyte detection device which comprises a specimen tube, a shell, a rotating seat, a driving motor clamping mechanism and a detection mechanism, wherein the specimen tube comprises a tube body, a tube cover and a base, the wall body of the tube body is made of transparent materials, the tube body is provided with a liquid storage bin, and the liquid storage bin is used for detecting the mixing of semen and n-toluidine cell peroxidase liquid; the inner part of the shell is provided with a cavity, the rotating seat is rotatably connected with the inner wall of the shell and provided with a plurality of fixing ports, the fixing ports penetrate through the rotating seat, and the base is fixed at the fixing ports through the clamping mechanism; the driving motor is used for driving the rotating seat; the detection mechanism comprises a camera, a picture identification module and a data processing module and is used for calculating the number of white blood cells in each 1mL of semen in the liquid storage bin. The invention can obtain the information of the concentration of the white blood cells in the semen in batches and improve the detection efficiency.

Description

Automatic semen leucocyte detection device

Technical Field

The invention relates to the technical field of medical machinery, in particular to an automatic semen leucocyte detection device.

Background

In the semen of most people, there are leukocytes, mainly polymorphonuclear leukocytes (PMNs, neutrophils). With a size of about 10-12 microns. The reference low value of peroxidase-positive polymorphonuclear leukocytes in normal fertile men is 0.5X 106/mL-1.0X 106/mL, and leukospermia is defined when the number of leukocytes is more than 1.0X 106/mL.

At present, the method is a primary screening experiment by adopting peroxidase staining of n-toluidine cells as semen leucocytes, the method is quick and cheap, and detection reagents and equipment are easy to purchase. However, in the method, the number of peroxidase positive cells is evaluated and calculated by using a blood cell counting pool, and an experimenter needs to quickly identify and count the positive cells under an optical microscope, so that the method has high requirement on the capability of the experimenter, needs long detection time and is not suitable for large sample size detection.

Disclosure of Invention

In order to solve the above problems, the present invention provides an automatic semen leukocyte detection device, which can obtain the leukocyte concentration information in semen in batch and improve the detection efficiency.

In order to achieve the purpose, the invention adopts the technical scheme that:

an automatic semen leucocyte detection device comprises a specimen tube, a shell, a rotating seat, a driving motor clamping mechanism and a detection mechanism,

the sample tube comprises a tube body, a tube cover and a base, wherein the interior of the tube body is of a hollow structure, the wall body is made of transparent materials, one end of the tube body is in threaded connection with the tube cover, and the other end of the tube body is fixedly connected with the base; a liquid storage bin is arranged in the tube body and comprises a guide part and a detection part, the guide part is of a funnel structure with openings at two ends, the peripheral wall of one end with a large aperture of the guide part is fixedly connected with the inner wall of one end, close to the tube cover, of the tube body, one end with a small aperture of the guide part is in conduction connection with the detection part, one end, far away from the guide part, of the detection part is of a sealing structure, the detection part is of a transparent tubular structure, and the outer wall of the detection part is provided with scales;

a cavity is arranged in the shell, and an opening communicated with the cavity is formed in the top of the shell; the rotating seat is arranged in the shell, a turntable bearing is sleeved on the outer wall of the rotating seat, the rotating seat is rotatably connected with the inner wall of the shell through the rotating bearing, the rotating seat is provided with a plurality of fixing ports which are arranged in a surrounding mode at intervals and penetrate through the rotating seat, and the base is fixed at the fixing ports through the clamping mechanism;

a driving shaft is fixedly arranged on one surface of the rotating seat, which is opposite to the opening of the shell, one end of the driving shaft, which is far away from the rotating seat, is rotatably connected with the bottom in the shell, and the driving motor is fixedly arranged at the inner bottom of the shell and is in transmission connection with the driving shaft through a gear set;

the driving motor is used for driving the sample tube to do centrifugal motion, white blood cells in the detected semen of the liquid storage bin are stained by n-toluidine cell peroxidase liquid and form purple peroxidase positive granulocytes, the density of the peroxidase positive granulocytes is higher than that of the semen, so that staining solution formed by the peroxidase positive granulocytes is deposited at the bottom of the detection part of the liquid storage bin, and the volume of the staining solution is obtained according to the scales;

the detection mechanism comprises a camera, a picture identification module and a data processing module,

the camera is fixedly arranged on the inner wall of the shell (2) and used for shooting each sample tube and sending a shot picture to the picture identification module;

the image identification module is used for acquiring volume reading of the staining solution in the sample tube in the image through a neural convolution network algorithm;

the data processing module is used for calculating the number of white blood cells in each 1mL of semen according to the volume reading of the picture recognition module, the concentration of the peroxidase positive granulocytes in the staining solution and the volume of the detected semen, wherein the concentration of the peroxidase positive granulocytes in the staining solution is a constant value and can be obtained through pre-calculation by a Haliotis diversicolor counting plate.

Furthermore, the fixing opening is of a circular truncated cone structure, one end of the small diameter of the fixing opening faces the opening of the shell, a clamping piece of the circular truncated cone structure is fixedly arranged at one end, away from the tube body, of the base, and the base is placed on the rotating seat so that the clamping piece can be embedded into the fixing opening;

the clamping mechanism is positioned in the shell and comprises clamping pieces, connecting seats, connecting discs and locking rings, the number of the connecting seats is the same as that of the fixing ports, at least three clamping pieces are arranged on each fixing port, the clamping pieces of each fixing port are vertically arranged and circumferentially arranged around the fixing port, one end of each clamping piece penetrates into the corresponding fixing port from one end with a large caliber of the fixing port, and the other end of each clamping piece is in sliding connection with the corresponding connecting seat; one side of the clamping piece, which is far away from the end part of the connecting seat, is provided with an abutting surface matched with the clamping piece, the other side of the clamping piece can be connected with the inner wall of the fixing opening in a sliding mode, and the abutting surface can abut against or leave from the clamping piece by vertically sliding the clamping piece along the inner wall of the fixing opening;

the center of the connecting seat is aligned with the axis of the fixing port, the center of one surface of the connecting seat, which is opposite to the clamping piece, is connected with the connecting disc in a sliding manner, the connecting disc is connected with the driving shaft in a rotating manner, a plurality of locking pieces are arranged on the side wall of the connecting disc in a surrounding manner, the shell is provided with a sliding port in sliding fit with the locking pieces, and the locking pieces penetrate through the shell and are connected with the sliding port in a sliding manner; the locking ring is sleeved on the outer wall of the shell in a threaded manner, and can push the locking piece through rotation on the shell, so that the locking piece is abutted against one end, close to the shell opening, of the sliding opening, the abutting surface of the clamping piece is abutted against the clamping piece, and the clamping piece is fixed in the fixing opening.

Furthermore, each connecting seat is provided with a sliding groove, one end, far away from the abutting surface, of the clamping piece is fixedly provided with a sliding block in sliding fit with the sliding groove, an abutting spring is arranged in the sliding groove, one end of the abutting spring is fixedly connected with the sliding block, the other end of the abutting spring is fixedly connected with the connecting seat, and under the elastic force action of the abutting spring, one side, back to the abutting surface, of the clamping piece is abutted against the inner wall of the fixing port.

Furthermore, one surface of the rotating seat facing the opening of the shell is provided with notches, the number of the notches is the same as that of the fixing ports, the notches are recessed on the peripheral ports of the fixing ports, and the notches are matched with the base.

Furthermore, the connecting plate is fixedly provided with a circular sliding rail, one surface of the connecting seat, which faces away from the clamping piece, is provided with a rolling bearing, the rolling bearing is arranged in the circular sliding rail, and the connecting seat is in sliding connection with the connecting plate through the rolling bearing.

Furthermore, the outer bottom of the shell is at least provided with three suckers, and the suckers are fixedly connected with the shell.

Furthermore, the detection mechanism also comprises an infrared generator, an infrared receiver, a centrifugal control module and a detection control module,

the infrared generator is fixedly arranged on the side surface of the rotating seat, and the back of the infrared generator faces to a certain fixing port; the infrared receiver is fixedly connected with the shell and is positioned in the vertical direction of the camera, and the infrared receiver is used for receiving signals of the infrared generator and calculating the distance between the infrared generator and the infrared receiver;

the centrifugal control module is used for controlling the driving motor so as to enable the sample tube to carry out centrifugal motion;

the detection control module comprises an initial position determining submodule and a shooting control submodule, the initial position determining submodule is used for controlling the driving motor to rotate, the initial position determining submodule can also obtain signals of the infrared receiver, and when the distance between the infrared generator and the infrared receiver is at the minimum value, the initial position determining submodule controls the driving motor to stop so as to enable the rotating seat to be at the initial position;

the shooting control submodule is used for acquiring the initial position determining submodule signal and can control the rotation angle of the driving motor and the shooting of the camera, after the shooting control submodule obtains a signal that the rotating seat in the initial position determining submodule is at the initial position, the shooting control submodule controls the driving motor to rotate by a preset angle after controlling the camera to shoot, so that the rotating seat rotates by a preset angle, the shooting control submodule repeatedly controls the shooting of the camera and the rotation of the driving motor, so that an image obtained by each shooting of the camera is just opposite to a certain sample tube, and when the shooting times of the camera are equal to the number of the fixed ports, the shooting control submodule controls the driving motor to stop rotating and the camera to stop shooting.

Further, the preset angle of the rotating seat is 360 degrees divided by the number of the fixing ports.

Furthermore, a coding frame is arranged on the sample tube and used for recording digital codes corresponding to the patient, and the picture identification module can also identify the digital codes in the pictures of the sample tube shot by the camera; the detection mechanism further comprises a table generation module, wherein the table generation module is used for acquiring the data processing module, matching the digital codes in each picture with the data of the number of the white blood cells and generating a table.

Further, the inclination of the inner wall of the fixing opening, the inclination of the outer wall of the clamping piece and the inclination of the abutting surface of the clamping piece are the same.

The invention has the beneficial effects that:

1. placing mixed liquid of detected semen and n-toluidine cell peroxidase liquid in a liquid storage bin, driving a rotating seat under the action of a driving motor to enable a specimen tube to rotate centrifugally, wherein under the centrifugal action, different particles in the semen have a sedimentation coefficient difference, and all the particles are settled at different speeds, so that zones are formed in different areas of density gradient. The guide part is of a funnel structure, and the peroxidase-positive granulocytes can fall into the detection part conveniently. The method comprises the steps that a sample tube is shot through a camera, a shot picture is sent to a picture recognition module, under the action of the picture recognition module, the liquid level height of a staining solution is recognized, the density of peroxidase positive granulocytes in different staining solutions is the same, the number of the peroxidase positive granulocytes in each 1mL of the staining solution can be calculated through a Bowden counting plate, the number of the peroxidase positive granulocytes in the detected semen is obtained through a data processing module according to the concentration of the peroxidase positive granulocytes in the staining solution and the volume of the staining solution, the number of the oxidase positive granulocytes in each 1mL of the semen is calculated according to the volume of the detected semen, and the number of the oxidase positive granulocytes is the number of the leukocytes because the oxidase positive granulocytes are staining substances of the leukocytes. The automatic detection of the concentration of the white blood cells in the semen is realized, and the working efficiency of inspectors is improved.

2. Twist and move the locking ring, it promotes the connection pad towards shell open-ended removal through the locking piece to move the locking ring to move for the connecting seat moves thereupon, the holder is under the elastic force effect of butt spring, the holder moves along fixed mouthful inner wall one side of butt face dorsad, when moving locking and slip mouthful butt, the butt face and the fastener butt of holder, make the fastener fixed in fixed mouthful, guarantee to rotate the seat when rotating, the mark pipe can be stably fixed and carry out centrifugal motion on rotating the seat. When the specimen tube needs to be taken out, the locking ring is screwed to move towards the bottom of the shell, and the locking piece is pressed down, so that the abutting surface of the clamping piece is separated from the clamping piece, and the base can be taken out from the fixing opening. Because the connecting seat is connected for rotating with the connection pad for when the connecting seat was driven by the rotation seat, the connecting seat can carry out circular motion on the connection pad, thereby realizes going on of sample pipe centrifugal motion.

3. Because the infrared receiver is positioned in the vertical direction of the camera and the back of the infrared generator faces a fixed port, when the infrared receiver receives a signal of the shortest distance of the infrared generator, the infrared receiver proves that the fixed port faces the camera, the initial position determining submodule controls the driving motor to stop so that the rotating seat is at the initial position, the shooting control submodule controls the camera to shoot after obtaining the information that the rotating seat is at the initial position so that the camera can shoot a front picture of a first standard pipe, the shooting control submodule controls the driving motor to rotate so that the rotating seat stops after rotating to a preset angle, the shooting control submodule controls the camera to shoot again so that the camera can shoot a front picture of a second standard pipe, and the shooting control submodule controls the shooting of the camera and the rotation of the driving motor repeatedly until the shooting times of the camera are equal to the number of the fixed ports, and ending the specimen tube picture acquisition process. Because the preset angle is 360 degrees and divided by the number of the fixed ports, the preset angle is rotated every time after the rotating seat is positioned at the initial position, the fixed ports on the rotating seat can be aligned with the cameras, the cameras can shoot complete and independently obtain the picture of the specimen tube every time, the recognition difficulty and the recognition time of the picture recognition module are reduced, and the recognition accuracy of the picture recognition module is improved.

Drawings

FIG. 1 is a schematic structural diagram of an automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 2 is a schematic structural diagram of a casing of an automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 3 is a schematic view of a rotating base of an automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 4 is a diagram of a connection disc structure of an automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 5 is a schematic diagram of a circular slide track structure of an automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 6 is a schematic view of a connecting seat structure of an automatic sperm leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 7 is a schematic view of a holder structure of an automatic semen leukocyte detection device according to a preferred embodiment of the invention.

FIG. 8 is a schematic diagram of the structure of the sample tube of the automatic semen leukocyte detection device according to a preferred embodiment of the present invention.

FIG. 9 is a block diagram of the structure of the detecting mechanism of the automatic semen leukocyte detecting device according to a preferred embodiment of the present invention.

In the figure, 1-specimen tube, 11-tube body, 12-tube cover, 13-base, 14-reservoir, 141-guide, 142-detection, 143-scale, 15-clip, 16-coding frame, 2-housing, 21-sliding port, 22-suction cup, 23-housing cover, 3-rotation base, 31-turntable bearing, 32-fixing port, 4-clamp, 401-abutment surface, 402-slider, 41-connection base, 411-sliding groove, 412-abutment spring, 413-rolling bearing, 42-connection plate, 421-locking piece, 422-circular sliding rail, 43-locking ring, 5-driving motor, 61-camera, 62-picture recognition module, 63-data processing module, 64-a table generation module, 71-an infrared generator, 72-an infrared receiver, 73-a centrifugal control module, 74-a detection control module, 741-an initial position determination sub-module, and 742-a shooting control sub-module.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1 to 8, an automatic semen leukocyte detection device according to a preferred embodiment of the present invention includes a sample tube 1, a housing 2, a rotating base 3, a driving motor 5, a clamping mechanism and a detection mechanism.

The specimen tube 1 comprises a tube body 11, a tube cover 12 and a base 13, wherein the tube body 11 is internally hollow and has a wall body made of transparent material, one end of the tube body 11 is in threaded connection with the tube cover 12, and the other end of the tube body is fixedly connected with the base 13.

Be equipped with stock solution storehouse 14 in the body 11, stock solution storehouse 14 includes guide 141 and detection part 142, guide 141 is both ends open-ended funnel structure, the perisporium of the one end of guide 141 large aperture and the inner wall fixed connection that body 11 is close to tube cap 12 one end, the one end and the detection part 142 turn-on connection in guide 141 small aperture, the one end that guide 141 was kept away from to detection part 142 is seal structure, detection part 142 is transparent tubular structure, and the outer wall is equipped with scale 143. The reservoir 14 is used for detecting the mixing of the semen and the n-toluidine cell peroxidase solution.

The inside cavity that is equipped with of shell 2, and the top is equipped with the opening that switches on with the cavity. Rotate seat 3 and locate in shell 2, and outer wall cover is equipped with swivel bearing 31, rotates seat 3 and rotates through the inner wall of rolling bearing 31 with shell 2 and be connected, rotates seat 3 and has seted up the alternate fixed mouthful 32 that encircles the setting and run through rotation seat 3 of a plurality of, and base 13 passes through fixture and fixes at fixed mouthful 32. The opening of the housing 2 is provided with a housing cover 23, and the housing cover 23 is screwed to the housing 2 and closes the opening of the housing 2.

The one side that rotates seat 3 and is dorsad the shell 2 opening sets firmly has drive shaft 51, and the one end that drive shaft 51 is kept away from and is rotated the bottom in seat 3 and the shell 2 and be connected, and driving motor 5 sets firmly in the interior bottom of shell 2, and is connected with drive shaft 51 transmission through gear train 52.

The driving motor 5 is used for driving the sample tube 1 to do centrifugal movement, white blood cells in the detected semen of the liquid storage bin 14 are stained by the n-toluidine cell peroxidase liquid, purple peroxidase positive granulocytes are formed, the density of the peroxidase positive granulocytes is higher than that of the semen, a staining solution formed by the peroxidase positive granulocytes is deposited at the bottom of the detection part 142 of the liquid storage bin 14, and the volume of the staining solution is obtained according to the scale 143.

Under the action of the driving motor 5, the rotating seat 3 is driven to enable the sample tube 1 to rotate centrifugally, under the centrifugal action, different particles in the semen have different sedimentation coefficients, and the particles are settled at different speeds, so that zones are formed on different areas of the density gradient, as white blood cells in the semen are stained by n-toluidine cell peroxidase liquid, purple peroxidase-positive granulocytes are generated, the density of the peroxidase-positive granulocytes is greater than that of the semen, and a staining solution formed by the peroxidase-positive granulocytes is settled at the bottom of the detection part 142 of the liquid storage bin 14. The guide section 141 has a funnel structure, and allows peroxidase-positive granulocytes to fall into the detection section 142.

In this embodiment, the fixing opening 32 is of a circular truncated cone structure, one end of the fixing opening 32 with a small diameter faces the opening of the housing 2, the clamping piece 15 of the circular truncated cone structure is fixedly arranged at one end of the base 13 far away from the tube body 11, and the base 13 is placed on the rotating base 3, so that the clamping piece 15 can be embedded into the fixing opening 32.

The clamping mechanism is located in the housing 2 and includes a clamping member 4, a connecting base 41, a connecting disc 42 and a locking ring 43.

The connecting seats 41 are the same as the fixing ports 32 in number, each fixing port 32 is at least provided with three clamping pieces 4, the clamping pieces 4 of each fixing port 32 are vertically placed and are arranged around the fixing port 32 in a surrounding mode, one end of each clamping piece 4 penetrates into the fixing port 32 from one end of the large caliber of the fixing port 32, and the other end of each clamping piece is connected with the corresponding connecting seat 41 in a sliding mode. One side of the clamping piece 4, which is far away from the end part of the connecting seat 41, is provided with an abutting surface 401 which is matched with the clamping piece 15, the other side of the clamping piece 4 can be in sliding connection with the inner wall of the fixed opening 32, and the abutting surface 401 can abut against the clamping piece 15 or leave the clamping piece 15 through vertically sliding the clamping piece 4 along the inner wall of the fixed opening 32.

The center of the connecting seat 41 is aligned with the axis of the fixing port 32, the center of one surface of the connecting seat 41, which is opposite to the clamping piece 4, is in sliding connection with the connecting disc 42, the connecting disc 42 is in rotating connection with the driving shaft 51, the side wall of the connecting disc 42 is provided with a plurality of locking pieces 421 in a surrounding manner, the shell 2 is provided with a sliding port 21 in sliding fit with the locking pieces 421, and the locking pieces 421 penetrate through the shell 2 to be in sliding connection with the sliding port 21; the locking ring 43 is screwed on the outer wall of the shell 2, the locking ring 43 can push the locking piece 421 by rotating on the shell 2, and the locking piece 421 is abutted against one end of the sliding opening 21 close to the opening of the shell 2, so that the abutting surface 401 of the clamping piece 4 is abutted against the clamping piece 15, and the clamping piece 15 is fixed in the fixed opening 32.

The locking ring 43 is screwed, the locking ring 43 pushes the connecting disc 42 to move towards the opening of the shell through the locking piece 421, so that the connecting seat 41 moves along with the connecting ring, the fixing port 32 is in a circular truncated cone structure, so that the clamping piece 4 can move upwards and enter a gap between the clamping piece 15 and the fixing port 32, when the locking ring 43 is abutted to the sliding port 21, the abutting surface 401 of the clamping piece 4 is abutted to the clamping piece 15, so that the clamping piece 15 is fixed in the fixing port 32, and the standard tube 1 can be stably fixed on the rotating seat 3 to perform centrifugal motion when the rotating seat 3 rotates.

When the sample tube 1 needs to be taken out, the locking ring 43 is screwed to move the locking ring 43 towards the bottom of the shell 2, and the locking piece 421 is pressed to enable the abutting surface 401 of the clamping piece 4 to be separated from the clamping piece 15, so that the base 13 of the sample tube 1 can be taken out from the fixing opening 32.

In this embodiment, each connecting seat 41 is provided with a sliding slot 411, one end of the clamping member 4 away from the abutting surface 401 is fixedly provided with a sliding block 402 in sliding fit with the sliding slot 411, an abutting spring 412 is arranged in the sliding slot 411, one end of the abutting spring 412 is fixedly connected with the sliding block 402, the other end of the abutting spring 412 is fixedly connected with the connecting seat 41, and under the elastic force action of the abutting spring 412, one side of the clamping member 4, which is opposite to the abutting surface 401, abuts against the inner wall of the fixing opening 32.

Under the elastic force of the abutting spring 412, when the clamping member 4 moves up and down, the side of the clamping member 4 opposite to the abutting surface 401 moves along the inner wall of the fixing opening 32, so that the clamping member 4 can be accurately embedded into the gap between the clamping piece 15 and the fixing opening 32 when the clamping member 4 moves up.

In this embodiment, the surface of the rotating base 3 facing the opening of the housing 2 is provided with notches 321, the number of the notches 321 is the same as the number of the fixing ports 32, the notches 321 are recessed on the peripheral port of the fixing ports 32, and the notches 321 are engaged with the base 13. Under the action of the notch 321, the card 15 can be aligned with the axis of the fixing opening 32, and the abutting surface 401 can abut against the card 15 when the clamping member 4 moves upwards.

Preferably, the inclination of the inner wall of the fixing opening 32, the inclination of the outer wall of the clamping piece 15 and the inclination of the abutting surface 401 of the clamping piece 4 are the same, so that two sides of the clamping piece 4 can be respectively connected with the clamping piece 15 and the fixing opening 32 in a sliding manner, and the clamping piece 4 can smoothly enter or leave the gap between the clamping piece 15 and the fixing opening 32.

Preferably, the connecting disc 42 is fixedly provided with a circular sliding rail 422, a rolling bearing 413 is arranged on one surface of the connecting seat 41, which faces away from the clamping piece 4, the rolling bearing 413 is arranged in the circular sliding rail 422, and the connecting seat 41 is slidably connected with the connecting disc 42 through the rolling bearing 413.

In this embodiment, the outer ring of the rolling bearing 413 is slidably connected to the ring sliding rail 422, the inner ring of the rolling bearing 413 is fixedly provided with a shaft body, and one end of the shaft body, which is far away from the rolling bearing 413, is fixedly connected to the connecting seat 41, so that the connecting seat 41 can perform circular motion on the connecting disc 42, and thereby the centrifugal motion of the sample tube 1 is performed.

Preferably, at least three suction cups 22 are disposed at the outer bottom of the housing 2, and the suction cups 22 are fixedly connected with the housing 2. Under the action of the suction cup 22, the stability of the housing 2 is improved, and the vibration caused by the rotation of the rotating base 3 is reduced.

The detection mechanism comprises a camera 61, a picture recognition module 62, a data processing module 63, an infrared generator 71, an infrared receiver 72, a centrifugal control module 73 and a detection control module 74.

The camera 61 is fixedly arranged on the inner wall of the shell (2) and used for shooting each sample tube 1 and sending the shot picture to the picture identification module 62.

The image recognition module 62 is used for obtaining the volume reading of the staining solution in the specimen tube 1 in the image through the neural convolution network algorithm.

The data processing module 63 is configured to calculate the number of leukocytes in each 1mL of semen according to the volume reading of the image recognition module 62, the concentration of peroxidase-positive granulocytes in the staining solution, and the volume of the detected semen, wherein the concentration of peroxidase-positive granulocytes in the staining solution is a constant value, and can be obtained by pre-calculation through a Haliotis diversicolor counting plate.

The sample tube is shot through the camera 61, the shot picture is sent to the picture recognition module 62, the volume of the staining solution is recognized under the action of the picture recognition module 62, and the number of peroxidase-positive granulocytes in each 1mL of staining solution can be calculated through the Bowden counting plate due to the fact that the density of peroxidase-positive granulocytes in different staining solutions is the same. The data processing module 63 obtains the number of the peroxidase-positive granulocytes in the detected semen according to the concentration of the peroxidase-positive granulocytes in the staining solution and the volume of the staining solution, and calculates the number of the peroxidase-positive granulocytes in each 1mL of the detected semen according to the volume of the detected semen, because the peroxidase-positive granulocytes are a staining substance of leukocytes, the number of the peroxidase-positive granulocytes is the number of the leukocytes. The automatic detection of the concentration of the white blood cells in the semen is realized, and the working efficiency of inspectors is improved.

The infrared generator 71 is fixedly arranged on the side surface of the rotating seat 3, and the back of the infrared generator 71 faces to a certain fixing port 32; the infrared receiver 72 is fixedly connected with the housing 2 and is located in the vertical direction of the camera 61, and the infrared receiver 72 is used for receiving the signal of the infrared generator 71 and calculating the distance between the infrared generator 71 and the infrared receiver 72.

The centrifugal control module 73 is used for control of the driving motor 5 to make the specimen tube 1 perform centrifugal motion.

The detection control module 74 includes an initial position determining sub-module 741 and a shooting control sub-module 742, the initial position determining sub-module 741 is configured to control the driving motor 5 to rotate, the initial position determining sub-module 741 is further configured to obtain a signal of the infrared receiver 72, and when the distance between the infrared generator 71 and the infrared receiver 72 is at a minimum value, the initial position determining sub-module 741 controls the driving motor 5 to stop so that the rotary base 3 is at an initial position.

The shooting control submodule 742 is configured to obtain a signal of the initial position determining submodule 741, and is capable of controlling a rotation angle of the driving motor 5 and shooting of the camera 61, after the shooting control submodule 742 obtains the signal that the rotating base 3 is at the initial position in the initial position determining submodule 741, the shooting control submodule 742 controls the camera 61 to shoot, and then controls the driving motor 5 to rotate by a preset angle, so that the rotating base 3 rotates by the preset angle, and the shooting control submodule 742 repeatedly controls shooting of the camera 61 and rotation of the driving motor 5, so that an image obtained by each shooting of the camera 61 is directly opposite to a certain sample tube 1, and when the number of times of shooting by the camera 61 is equal to the number of the fixed ports 32, the shooting control submodule 742 controls the driving motor 5 to stop rotating and the camera 61 to stop shooting.

In this embodiment, the preset angle of the rotating base 3 is 360 ° divided by the number of the fixing openings 32. The number of the fixing ports 32 is 5 in the present embodiment, and thus the preset angle of the rotary holder 3 is 72 °.

Since the infrared receiver 72 is located in the vertical direction of the camera 61 and the back of the infrared generator 71 is facing to a certain fixed port 32, when the infrared receiver 72 is in a signal of the shortest distance to the infrared generator 71, it is proved that a certain fixed port 32 is facing to the camera 61, the initial position determining submodule 741 controls the driving motor to stop so that the rotary seat 3 is at the initial position, the photographing control submodule 742 controls the camera 61 to photograph after obtaining the information that the rotary seat 3 is at the initial position so that the camera 61 can photograph the front picture of the first sample tube 1, the photographing control submodule 742 controls the driving motor 5 to rotate so that the rotary seat 3 stops after rotating to a preset angle, the photographing control submodule 742 controls the camera 61 to photograph again so that the camera 61 can photograph the front picture of the second sample tube 1, the photographing control submodule 742 repeatedly controls the photographing of the camera 61 and the driving motor 5 to rotate, and ending the process of acquiring the picture of the sample tube 1 until the shooting times of the camera 61 are equal to the number of the fixed ports 32.

Because the preset angle is 360 degrees and is divided by the number of the fixed ports, the rotating seat 3 rotates the preset angle every time after being positioned at the initial position, the fixed ports 32 on the rotating seat 3 can be aligned to the cameras 61, the cameras 61 can shoot a complete picture of the specimen tube 1 independently every time, the recognition difficulty and the recognition time of the picture recognition module 62 are reduced, and the recognition accuracy of the picture recognition module 62 is improved.

In this embodiment, the sample tube 1 is provided with the encoding frame 16, the encoding frame 16 is used for recording the digital code corresponding to the patient, and the picture identification module 62 is further capable of identifying the digital code in the picture of the sample tube 1 taken by the camera 61. The detection mechanism further comprises a table generation module 64, and the table generation module 64 is used for acquiring the data processing module 63, matching the digital code in each picture with the concentration data of the white blood cells, and generating a table. The number of the leucocytes in the semen of the patient can be rapidly obtained by the tester through the table generating module 64, so that whether the patient belongs to the leucocytozoonosis or not can be rapidly judged.

In this embodiment, the image recognition module 62, the data processing module 63, and the table generation module 64 are all computer modules, and the image recognition module 62 obtains image data of the camera 61 through a data line and performs volume reading processing of the dyeing solution on the computer. The data processing module 63 processes the data of the picture recognition module 62 through a computer. The table generating module 64 obtains the data of the picture identifying module 62 by the computer.

Centrifugal control module 73 installs on the outer wall of shell 2, and is connected with driving motor 5 electricity, and centrifugal control module 73 is equipped with centrifugal control button, through pressing centrifugal control button for driving motor 5 is controlled by detection control module 74, and detection control module 74 passes through single chip microcomputer control driving motor 5.

Detect control module 74 and install on the outer wall of shell 2, and be connected with driving motor 5 and camera 61 electricity, detect control module 74 and be equipped with and detect control button for driving motor 5 and camera 61 are controlled by detection control module 74 through pressing detection control button. The detection control module 74 controls the driving motor 5 and the camera 6 through a single chip microcomputer,

the steps of detecting the number of the semen leukocytes by using the automatic semen leukocyte detection device in a preferred embodiment of the present embodiment are as follows:

s1, taking 1mL of semen of the patient and 0.9mL of n-toluidine cell peroxidase liquid, putting the semen and the n-toluidine cell peroxidase liquid into a liquid storage bin 14, recording the digital code of the patient on a coding frame 16, covering a tube cover 12 on a tube body 11, shaking uniformly for 10 seconds, and incubating for 20-30 minutes at room temperature.

S2, the locking ring 43 is screwed to move the locking ring 43 toward the bottom of the housing 2, and the abutting surface 401 is lowered to the end of the fixing port 32 near the bottom of the housing 2 by pressing down the locking member 421, and the specimen tube 1 in the step S1 is fitted into the fixing port 32 with the base 13 aligned with the notch 321.

S3, screwing the locking ring 43, the locking ring 43 pushes the connecting disc 42 to move towards the opening of the shell through the locking piece 421, under the action of the abutting spring 412, the side of the clamping piece 4 opposite to the abutting surface 401 moves upwards along the inner wall of the fixed opening 32, so that the clamping piece 4 can move upwards and enter the gap between the clamping piece 15 and the fixed opening 32, screwing the locking ring 43 until the locking ring 43 abuts against the sliding opening 21, so that the abutting surface 401 of the clamping piece 4 abuts against the clamping piece 15, and the clamping piece 15 is fixed in the fixed opening 32.

And S4, controlling the rotation of the driving motor 5 through the centrifugal control module 73 so as to enable the sample tube 1 to carry out centrifugal motion. After the centrifugal movement of the sample tube 1 is finished, the white blood cells in the detection semen of the liquid storage bin 14 are stained by the n-toluidine cell peroxidase liquid, and form purple peroxidase-positive granulocytes, and meanwhile, the staining solution formed by the peroxidase-positive granulocytes sinks to the bottom of the detection part 142 of the liquid storage bin 14.

S5, the initial position determination sub-module 741 controls the driving motor 5 to rotate, and when the distance between the infrared generator 71 and the infrared receiver 72 is at a minimum value, the initial position determination sub-module 741 controls the driving motor 5 to stop so that the rotary table 3 is at the initial position.

S6, the shooting control submodule 742 controls the camera 61 to shoot after obtaining the information that the rotating seat 3 is at the initial position, firstly the camera 61 shoots the front picture of the first sample tube 1, the shooting control submodule 742 controls the driving motor 5 to rotate, so that the rotating seat 3 stops rotating to a preset angle, the shooting control submodule 742 controls the camera 61 to shoot again, the camera 61 shoots the front picture of the second sample tube 1, the shooting control submodule 742 controls the shooting of the camera 61 and the rotation of the driving motor 5 repeatedly, and the picture obtaining process of the sample tube 1 is finished until the shooting times of the camera 61 are equal to the number of the fixed ports 32.

S7, the picture recognition module 62 obtains the volume reading of the staining solution in the sperm tube 1 and the digital code of the coding frame 16 in the picture through a neural convolution network algorithm, and sends the volume reading of the staining solution and the data of the digital code of the coding frame 16 to the data processing module 63, and the data processing module 63 calculates the number of the white blood cells in each 1mL of the semen according to the volume reading of the picture recognition module 62, the concentration of the peroxidase positive granulocytes in the staining solution and the volume of the detected semen.

S8, the table generating module 64 acquires the data processing module 63, matches the digital code in each picture with the data of the number of white blood cells, and generates the table.

Claims

1. An automatic semen leucocyte detection device is characterized by comprising a specimen tube (1), a shell (2), a rotating seat (3), a driving motor (5), a clamping mechanism and a detection mechanism,

the sample tube (1) comprises a tube body (11), a tube cover (12) and a base (13), wherein the interior of the tube body (11) is of a hollow structure, the wall body of the tube body is made of transparent materials, one end of the tube body (11) is in threaded connection with the tube cover (12), and the other end of the tube body is fixedly connected with the base (13); a liquid storage bin (14) is arranged in the tube body (11), the liquid storage bin (14) comprises a guide part (141) and a detection part (142), the guide part (141) is of a funnel structure with openings at two ends, the peripheral wall of one end with a large aperture of the guide part (141) is fixedly connected with the inner wall of one end, close to the tube cover (12), of the tube body (11), one end with a small aperture of the guide part (141) is in conduction connection with the detection part (142), one end, far away from the guide part (141), of the detection part (142) is of a sealing structure, the detection part (142) is of a transparent tubular structure, and scales (143) are arranged on the outer wall of the detection part (142);

a cavity is arranged in the shell (2), and an opening communicated with the cavity is formed in the top of the shell; the rotating seat (3) is arranged in the shell (2), a turntable bearing (31) is sleeved on the outer wall of the rotating seat, the rotating seat (3) is rotatably connected with the inner wall of the shell (2) through the rotating bearing (31), the rotating seat (3) is provided with a plurality of fixing ports (32) which are alternately arranged in a surrounding mode and penetrate through the rotating seat (3), and the base (13) is fixed on the fixing ports (32) through the clamping mechanism;

a driving shaft (51) is fixedly arranged on one surface, back to the opening of the shell (2), of the rotating seat (3), one end, far away from the rotating seat (3), of the driving shaft (51) is rotatably connected with the bottom in the shell (2), and the driving motor (5) is fixedly arranged at the inner bottom of the shell (2) and is in transmission connection with the driving shaft (51) through a gear set (52);

the driving motor (5) is used for driving the sample tube (1) to do centrifugal motion, white blood cells in the detected semen of the liquid storage bin (14) are stained by n-toluidine cell peroxidase liquid, purple peroxidase positive granulocytes are formed, the density of the peroxidase positive granulocytes is higher than that of the semen, a staining solution formed by the peroxidase positive granulocytes is deposited at the bottom of a detection part (142) of the liquid storage bin (14), and the volume of the staining solution is obtained according to the scales (143);

the detection mechanism comprises a camera (61), a picture identification module (62) and a data processing module (63),

the camera (61) is fixedly arranged on the inner wall of the shell (2) and is used for shooting each sample tube (1) and sending a shot picture to the picture identification module (62);

the picture identification module (62) is used for acquiring volume reading of the staining solution in the sample tube (1) in the picture through a neural convolution network algorithm;

the data processing module (63) is used for calculating the number of white blood cells in each 1mL of semen according to the volume reading of the picture recognition module (62), the concentration of the peroxidase positive granulocytes in the staining solution and the volume of the detected semen, wherein the concentration of the peroxidase positive granulocytes in the staining solution is a constant value and can be obtained through pre-calculation by a Haliotis diversicolor counting plate.

2. The semen leukocyte detection device of claim 1, wherein: the fixing opening (32) is of a circular truncated cone structure, one end of the small diameter of the fixing opening (32) faces the opening of the shell (2), a clamping piece (15) of the circular truncated cone structure is fixedly arranged at one end, away from the pipe body (11), of the base (13), and the base (13) is placed on the rotating seat (3) so that the clamping piece (15) can be embedded into the fixing opening (32);

the clamping mechanism is positioned in the shell (2) and comprises clamping pieces (4), connecting seats (41), connecting discs (42) and locking rings (43), the number of the connecting seats (41) is the same as that of the fixing ports (32), at least three clamping pieces (4) are arranged on each fixing port (32), the clamping pieces (4) of each fixing port (32) are vertically placed and arranged around the fixing port (32) in a surrounding mode, one end of each clamping piece (4) penetrates into the fixing port (32) from one end with a large caliber of the fixing port (32), and the other end of each clamping piece is connected with the corresponding connecting seat (41) in a sliding mode; one side, away from the end part of the connecting seat (41), of the clamping piece (4) is provided with an abutting surface (401) matched with the clamping piece (15), the other side of the clamping piece can be in sliding connection with the inner wall of the fixing opening (32), and the abutting surface (401) can abut against the clamping piece (15) or leave the clamping piece (15) by vertically sliding the clamping piece (4) along the inner wall of the fixing opening (32);

the center of the connecting seat (41) is aligned with the axis of the fixing port (32), the center of one surface, back to the clamping piece (4), of the connecting seat (41) is connected with the connecting disc (42) in a sliding mode, the connecting disc (42) is connected with the driving shaft (51) in a rotating mode, a plurality of locking pieces (421) are arranged on the side wall of the connecting disc (42) in a surrounding mode, the shell (2) is provided with a sliding port (21) in sliding fit with the locking pieces (421), and the locking pieces (421) penetrate through the shell (2) to be connected with the sliding port (21) in a sliding mode; the locking ring (43) is sleeved on the outer wall of the shell (2) in a threaded mode, the locking ring (43) can push the locking piece (421) through rotating on the shell (2), and the locking piece (421) is abutted to one end, close to the opening of the shell (2), of the sliding opening (21), so that the abutting surface (401) of the clamping piece (4) is abutted to the clamping piece (15), and the clamping piece (15) is fixed in the fixing opening (32).

3. The semen leukocyte detection device of claim 2, wherein: each connecting seat (41) is equipped with sliding tray (411), holder (4) are kept away from the one end of butt face (401) set firmly with sliding tray (411) sliding fit's slider (402), be equipped with butt spring (412) in sliding tray (411), butt spring (412) one end slider (402) fixed connection, the other end with connecting seat (41) fixed connection under the spring action of butt spring (412), holder (4) dorsad the one side of butt face (401) with fixed mouthful (32) inner wall butt.

4. The device for the automatic detection of semen leucocytes according to claim 2, characterized in that: one surface of the rotating seat (3) facing the opening of the shell (2) is provided with notches (321), the number of the notches (321) is the same as that of the fixing ports (32), the notches are recessed on the peripheral ports of the fixing ports (32), and the notches (321) are matched with the base (13).

5. The device for the automatic detection of semen leucocytes according to claim 2, characterized in that: the connecting disc (42) is fixedly provided with a circular ring sliding rail (422), the connecting seat (41) faces away from one surface of the clamping piece (4) and is provided with a rolling bearing (413), the rolling bearing (413) is arranged in the circular ring sliding rail (422), and the connecting seat (41) is connected with the connecting disc (42) in a sliding mode through the rolling bearing (413).

6. The device for the automatic detection of semen leucocytes according to claim 1, characterized in that: the outer bottom of shell (2) is equipped with three sucking disc (22) at least, sucking disc (22) with shell (2) fixed connection.

7. The device for the automatic detection of semen leucocytes according to claim 1, characterized in that: the detection mechanism also comprises an infrared generator (71), an infrared receiver (72), a centrifugal control module (73) and a detection control module (74),

the infrared generator (71) is fixedly arranged on the side surface of the rotating seat (3), and the back of the infrared generator (71) faces to one fixing port (32); the infrared receiver (72) is fixedly connected with the shell (2) and is positioned in the vertical direction of the camera (61), and the infrared receiver (72) is used for receiving signals of the infrared generator (71) and calculating the distance between the infrared generator (71) and the infrared receiver (72);

the centrifugal control module (73) is used for controlling the driving motor (5) so as to enable the sample tube (1) to carry out centrifugal motion;

the detection control module (74) comprises an initial position determining submodule (741) and a shooting control submodule (742), the initial position determining submodule (741) is used for controlling the driving motor (5) to rotate, the initial position determining submodule (741) can also acquire signals of the infrared receiver (72), and when the distance between the infrared generator (71) and the infrared receiver (72) is at a minimum value, the initial position determining submodule (741) controls the driving motor (5) to stop so that the rotating seat (3) is at an initial position;

the shooting control submodule (742) is configured to obtain a signal of the initial position determining submodule (741), and is capable of controlling a rotation angle of the driving motor (5) and shooting of the camera (61), when the shooting control submodule (742) obtains a signal that the rotating seat (3) in the initial position determining submodule (741) is at an initial position, the shooting control submodule (742) controls the camera (61) to shoot, and then controls the driving motor (5) to rotate by a preset angle, so that the rotating seat (3) rotates by a preset angle, the shooting control submodule (742) repeatedly controls shooting of the camera (61) and rotation of the driving motor (5), so that an image obtained by each shooting of the camera (61) is just opposite to a certain sample tube (1), and when the shooting frequency of the camera (61) is equal to the number of the fixed ports (32), the shooting control submodule (742) controls the driving motor (5) to stop rotating and the camera (61) to stop shooting.

8. The device for the automatic detection of semen leucocytes according to claim 7, characterized in that: the preset angle of the rotating seat (3) is 360 degrees divided by the number of the fixing ports (32).

9. The device for the automatic detection of semen leucocytes according to claim 1, characterized in that: the sample tube (1) is provided with a coding frame (16), the coding frame (16) is used for recording digital codes corresponding to patients, and the picture identification module (62) can also identify the digital codes in the pictures of the sample tube (1) shot by the camera (61); the detection mechanism further comprises a table generation module (64), wherein the table generation module (64) is used for acquiring the data processing module (63), matching the digital codes in each picture with the data of the number of the white blood cells and generating a table.

10. The device for the automatic detection of semen leucocytes according to claim 1, characterized in that: the inclination of the inner wall of the fixing opening (32), the inclination of the outer wall of the clamping piece (15) and the inclination of the abutting surface (401) of the clamping piece (4) are the same.